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Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences.
Sretenovic S, Yin D, Levav A, Selengut JD, Mount SM, Qi Y
Plant Commun. 2020 Jul 22;2(2):100101. doi: 10.1016/j.xplc.2020.100101. eCollection 2021 Mar 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
149553pYPQ166-iSpyMacGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
149554pYPQ265HGateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs
149555pYPQ266BGateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs
149556pYPQ262m3Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

Antibodies from Article