A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi.
Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C
Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. PubMed Article
Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C
Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118229 | pBSV2H | Empty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant |
| 149557 | pKFSS1_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149558 | pBSV2B_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149559 | pBSV2H_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149560 | pBSV2G_Psyn-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149561 | pBSV2H_Psyn-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149562 | pBSV2G_Psyn-sgRNAftsI | ftsI gRNA expression in B. burgdorferi |
| 149563 | pBSV2H_Psyn-sgRNAftsI | ftsI gRNA expression in B. burgdorferi |
| 149566 | pBSV2G_Psyn-sgRNAmreB | mreB gRNA expression in B. burgdorferi |
| 149567 | pBSV2H_Psyn-sgRNAmreB | mreB gRNA expression in B. burgdorferi |
| 149568 | pBSV2G_Psyn-sgRNArodA | rodA gRNA expression in B. burgdorferi |
| 149569 | pBSV2H_Psyn-sgRNArodA | rodA gRNA expression in B. burgdorferi |
| 149570 | pBbdCas9K_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149571 | pBbdCas9G_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149572 | pBbdCas9B_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149573 | pBbdCas9H_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149574 | pBbdCas9S(RBSmut) | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149575 | pBbdCas9S(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149576 | pBbdCas9S(RBSmut)_Psyn-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149577 | pBbdCas9K(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149578 | pBbdCas9G(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149579 | pBbdCas9B(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149580 | pBbdCas9H(RBSmut)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149581 | pBbdCas9S(-10TC) | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149582 | pBbdCas9S(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149583 | pBbdCas9S(-10TC)_Psyn-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149584 | pBbdCas9K(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149585 | pBbdCas9G(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149586 | pBbdCas9B(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149587 | pBbdCas9H(-10TC)_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149588 | pBbdCas9S(RBSmut)_Psyn-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149589 | pBbdCas9S(-10TC)_Psyn-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149590 | pBbdCas9S(RBSmut)_Psyn-sgRNAftsI | all-in-one CRISPRi vector for targeting B. burgdorferi ftsI |
| 149591 | pBbdCas9S(-10TC)_Psyn-sgRNAftsI | all-in-all CRISPRi vector for targeting B. burgdorferi ftsI |
| 149594 | pBbdCas9S(RBSmut)_Psyn-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
| 149595 | pBbdCas9S(-10TC)_Psyn-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
| 149613 | pBSV2_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149614 | pBSV2G_Psyn-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149615 | pBSV2G_PflaBS-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149616 | pBSV2G_PresTS-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149617 | pBSV2G_PresTL-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149618 | pBSV2G_P0826S-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149619 | pBSV2G_P0826L-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149620 | pBSV2G_P0026-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149621 | pBSV2G_P0526-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149622 | pBSV2G_P0031-sgRNA500 | gRNA expression in B. burgdorferi, parental vector |
| 149623 | pBSV2G_PflaBS-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149624 | pBSV2G_PresTS-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149625 | pBSV2G_PresTL-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149626 | pBSV2G_P0826S-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149627 | pBSV2G_P0826L-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149628 | pBSV2G_P0026-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149629 | pBSV2G_P0526-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149630 | pBSV2G_P0031-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149631 | pBSV2_Psyn-sgRNAflaB | flaB gRNA expression in B. burgdorferi |
| 149632 | pBSV2_Psyn-sgRNAftsI | ftsI gRNA expression in B. burgdorferi |
| 149634 | pBSV2_Psyn-sgRNAmreB | mreB gRNA expression in B. burgdorferi |
| 149635 | pBSV2_Psyn-sgRNArodA | rodA gRNA expression in B. burgdorferi |
| 149636 | pBSV2_Psyn-mCherryBb | Psyn-mCherry promoter strength reporter for use in B. burgdorferi |
| 149637 | pKIKan_idCas9_Chr_center | suicide vector for knock in into the B. burgdorferi chromosome |
| 149638 | pBbdCas9S | parental, all-in-one CRISPRi vector for B. burgdorferi, empty |
| 149639 | pBbdCas9S_arr2 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149640 | pBbdCas9S_Psyn-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149641 | pBbdCas9S_P0526-sgRNA500 | parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning |
| 149642 | pBbdCas9S_Psyn-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149643 | pBbdCas9S_PflaBS-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149644 | pBbdCas9S_PresTS-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149645 | pBbdCas9S_PresTL-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149646 | pBbdCas9S_P0026-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149647 | pBbdCas9S_P0826S-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149648 | pBbdCas9S_P0826L-sgRNAflaB | all-in-one CRISPRi vector for targeting B. burgdorferi flaB |
| 149649 | pBbdCas9S_Psyn-sgRNAftsI | all-in-one CRISPRi vector for targeting B. burgdorferi ftsI |
| 149650 | pBbdCas9S_P0526-sgRNAftsI | all-in-one CRISPRi vector for targeting B. burgdorferi ftsI |
| 149653 | pBbdCas9S_Psyn-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
| 149654 | pBbdCas9S_P0526-sgRNAmreB | all-in-one CRISPRi vector for targeting B. burgdorferi mreB |
| 149655 | pBbdCas9S_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
| 149656 | pBbdCas9S(RBSmut)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
| 149657 | pBbdCas9S(-10TC)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
| 149658 | pBbdCas9S_P0526-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
| 149659 | pBbdCas9S(-10AC1)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
| 149660 | pBbdCas9S(-10AC2)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |
| 149661 | pBbdCas9S(-10AC12)_Psyn-sgRNArodA | all-in-one CRISPRi vector for targeting B. burgdorferi rodA |