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Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance to PD-1 blockade.
Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 10.1158/2159-8290.CD-19-1416.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
160090LentiGuide-hygroExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker
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160091LentiGuide-zeoExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker
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160092pLX304_zeoLentiviral vector for mammalian gene expression
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160093pLX304_zeo_emptyControl lentiviral vector for mammalian gene expression
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160094pDONR221_eGFP (open)Expresses eGFP in mammalian cells
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160095pLX304_zeo_eGFPExpresses eGFP in mammalian cells
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160096pLX304_zeo_eGFP_V5Expresses eGFP in mammalian cells
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160097pDONR221_mmIrf1 (closed)Expresses murine Irf1 in mammalian cells
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160098pLX304_zeo_mmIrf1Expresses murine Irf1 in mammalian cells
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160099pENTR1A_Mavs (open)Expresses murine Mavs in mammalian cells
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160100pLX304_zeo_mmMavs_V5 Expresses murine Mavs in mammalian cells
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160101pENTR1A_mmFbxw7a (closed)Expresses murine Fbxw7 alpha in mammalian cells
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160102pLX304_zeo_mmFbxw7aExpresses murine Fbxw7 alpha in mammalian cells
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160103pENTR1A_mmFbxw7_R505C (closed)Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells
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160104pLX304_zeo_mmFbxw7_R505CExpresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells
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160105pENTR1A_mmFbxw7(res) (closed)Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA
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160106pLX304_zeo_mmFbxw7(res)Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA
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160107pENTR223.1_mmDdx58 (closed)Expresses murine Ddx58 in mammalian cells
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160108pLenti6.2_3xFLAG_V5_ mmDdx58_V5Expresses murine Ddx58 in mammalian cells
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160110pLenti6.2_3xFLAG_V5_ mmIfih1_V5Expresses murine Ifih1 in mammalian cells
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160111pLenti6.2_3xFLAG_V5_ eGFP_V5Expresses eGFP in mammalian cells
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Antibodies from Article