Self-Cutting and Integrating CRISPR Plasmids Enable Targeted Genomic Integration of Genetic Payloads for Rapid Cell Engineering.
Bloemberg D, Sosa-Miranda CD, Nguyen T, Weeratna RD, McComb S
CRISPR J. 2021 Feb;4(1):104-119. doi: 10.1089/crispr.2020.0090. PubMed Article
Bloemberg D, Sosa-Miranda CD, Nguyen T, Weeratna RD, McComb S
CRISPR J. 2021 Feb;4(1):104-119. doi: 10.1089/crispr.2020.0090. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154090 | pQCi-mB2m-sgRNA | pQCi backbone with sgRNA targeting murine Beta-2-microglobulin |
| 154091 | pSCIP-mB2M | Self-Cutting and Integrating CRISPR backbone targeting murine B2M |
| 154093 | pQCi-hTRAC-sgRNA | pQCi backbone with sgRNA targeting human TCR-alpha common chain |
| 154094 | pSCIP-hTRAC | Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain |
| 154095 | pSCIP-hCD69-TdTomato | Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene |
| 154096 | pSCIPpay-BFP reporter | Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter |
| 154345 | pQCi | Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites) |