CRTC1/MAML2 directs a PGC-1alpha-IGF-1 circuit that confers vulnerability to PPARgamma inhibition.
Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL
Cell Rep. 2021 Feb 23;34(8):108768. doi: 10.1016/j.celrep.2021.108768. PubMed Article
Musicant AM, Parag-Sharma K, Gong W, Sengupta M, Chatterjee A, Henry EC, Tsai YH, Hayward MC, Sheth S, Betancourt R, Hackman TG, Padilla RJ, Parker JS, Giudice J, Flaveny CA, Hayes DN, Amelio AL
Cell Rep. 2021 Feb 23;34(8):108768. doi: 10.1016/j.celrep.2021.108768. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154259 | pPGC1a_Proximal Promoter-FLuc2P | Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52. |
| 154260 | pPGC1a_Distal Promoter-FLuc2P | Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52. |
| 154261 | pIGF1_P1 Promoter-FLuc2P | Destabilized firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter. |
| 154262 | pIGF1_P2 Promoter-FLuc2P | Destabilized firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter. |
| 154263 | pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro | Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells. |
| 154264 | pRetroX-Tight_A-CREB_PGK-tdTomato | Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells. |
| 154265 | pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc | Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells. |
| 154266 | pIGF1_P1 Promoter-FLuc | Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter. |
| 154267 | pIGF1_P2 Promoter-FLuc | Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter. |
| 165425 | pSico_U6-PGC1a1 sgRNA | Expression of gRNA against human PGC-1a variant 1 |
| 165426 | pSico_U6-Pan-PGC1a sgRNA | Expression of gRNA against human Total PGC-1a variants |