Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism.
Aregger M, Lawson KA, Billmann M, Costanzo M, Tong AHY, Chan K, Rahman M, Brown KR, Ross C, Usaj M, Nedyalkova L, Sizova O, Habsid A, Pawling J, Lin ZY, Abdouni H, Wong CJ, Weiss A, Mero P, Dennis JW, Gingras AC, Myers CL, Andrews BJ, Boone C, Moffat J
Nat Metab. 2020 Jun;2(6):499-513. doi: 10.1038/s42255-020-0211-z. Epub 2020 Jun 1. PubMed Article
Aregger M, Lawson KA, Billmann M, Costanzo M, Tong AHY, Chan K, Rahman M, Brown KR, Ross C, Usaj M, Nedyalkova L, Sizova O, Habsid A, Pawling J, Lin ZY, Abdouni H, Wong CJ, Weiss A, Mero P, Dennis JW, Gingras AC, Myers CL, Andrews BJ, Boone C, Moffat J
Nat Metab. 2020 Jun;2(6):499-513. doi: 10.1038/s42255-020-0211-z. Epub 2020 Jun 1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155085 | pLCKO_AAVS1_sgRNA_2 | lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus |
| 155086 | pLCKO_AAVS1_sgRNA_3 | lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus |
| 155087 | pLCKO_AAVS1_sgRNA_4 | lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus |
| 155088 | LCV2_AAVS1_sgRNA_2 | lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus |
| 155089 | LCV2_AAVS1_sgRNA_3 | lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus |
| 155090 | LCV2_AAVS1_sgRNA_4 | lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus |
| 155091 | LCV2_PSMD1_sgRNA_1 | lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene) |
| 155092 | LCV2_PSMB2_sgRNA_1 | lentiviral plasmid expressing Cas9 and gRNA targeting PSMB2 (core essential gene) |
| 155093 | LCV2_LacZ_sgRNA_2 | lentiviral plasmid expressing Cas9 and gRNA targeting LacZ |
| 155094 | LCV2_Luciferase_sgRNA | lentiviral plasmid expressing Cas9 and gRNA targeting Luciferase |
| 155095 | LCV2_mClover3 | Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression |
| 155096 | LCV2_mCherry | Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression |
| 155097 | LCV2_BFP2 | Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression |
| 155098 | LCV2_EGFP | Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression |
| 155099 | LCV2_mClover3_AAVS1_sgRNA_2 | lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus |
| 155101 | LCV2_mClover3_AAVS1_sgRNA_4 | lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus |
| 155102 | LCV2_mClover3_PSMD1_sgRNA_1 | lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene) |
| 155103 | LCV2_mClover3_LacZ | lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ |
| 155105 | LCV2_mCherry_AAVS1_sgRNA_2 | lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus |
| 155106 | LCV2_mCherry_AAVS1_sgRNA_4 | lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus |
| 155107 | LCV2_mCherry_PSMD1_sgRNA_1 | lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene) |
| 155108 | LCV2_mCherry_LacZ_sgRNA | lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ |
| 155109 | LCV2_mCherry_Luciferase_sgRNA | lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase |
| 155110 | pLX304_C12orf49-V5 | lentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49 |
| 155111 | pCDNA5_miniTurbo-C12orf49 | transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID |
| 155112 | pCDNA5_C12orf49-miniTurbo | transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID |