Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase.
Wilson C, Chen PJ, Miao Z, Liu DR
Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6. PubMed Article
Wilson C, Chen PJ, Miao Z, Liu DR
Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155366 | pCMV-dCas13-M3nls | Targeted m6A RNA methylation in mammalian cells |
| 155367 | pCMV-dCas13-M3M14nes | Targeted m6A RNA methylation in mammalian cells |
| 155368 | pU6-PspCas13b-gRNA-Actb1216 | PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation |
| 157854 | pCMV-dCas13-inactive M3nls | Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant |
| 157855 | pCMV-dCas13-inactive M3M14nes | Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant |