A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis.
Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19. PubMed Article
Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158713 | pNHEJ-Cas12a | CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum |
| 158714 | pNHEJ-Cas12a-recX | CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX |
| 158715 | pNHEJ-Cas9-recX | CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX |
| 158717 | pNHEJ-recAmu-sacB | CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele. |
| 158718 | pNHEJ-recX-sacB | CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX. |
| 158719 | pYC1640 | CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker. |
| 158720 | pYC1446 | Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA |
| 158721 | pYC2085 | CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker. |
| 158722 | pYC1876 | Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA |