Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or Neurog2 activity.
Kaufman ML, Goodson NB, Park KU, Schwanke M, Office E, Schneider SR, Abraham J, Hensley A, Jones KL, Brzezinski JA
Development. 2021 Jun 15;148(12). pii: 269198. doi: 10.1242/dev.199399. Epub 2021 Jun 18. PubMed Article
Please see Addgene's curated Brzezinski Lab CRISPR Collection page for additional information about this set of plasmids.
Kaufman ML, Goodson NB, Park KU, Schwanke M, Office E, Schneider SR, Abraham J, Hensley A, Jones KL, Brzezinski JA
Development. 2021 Jun 15;148(12). pii: 269198. doi: 10.1242/dev.199399. Epub 2021 Jun 18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159653 | pMin-DHS-4D-GFP | Reporter plasmid for the DHS-4D Otx2 enhancer element. |
| 159654 | pX458-Ef1a-Cas9-H2B-GFP | Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter. |
| 159655 | pX458-Ef1a-Cas9-H2B-mCherry | Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter. |
| 159656 | pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1. |
| 159657 | pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1. |
| 159658 | pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1. |
| 159659 | pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2. |
| 159660 | pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2. |
| 159661 | pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2. |
| 159662 | pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2. |
| 159663 | pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2. |
| 159664 | pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3) | CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2. |
| 171098 | pX458-Dual-Guide-Donor-ShuttleOnly | Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI. |
| 171099 | pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2) | CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1. |
| 171100 | pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2) | CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2. |
| 171101 | pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2) | CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2. |
| 171102 | pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2) | CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2. |