An optimized toolbox for the optogenetic control of intracellular transport.
Nijenhuis W, van Grinsven MMP, Kapitein LC
J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149. PubMed Article
Nijenhuis W, van Grinsven MMP, Kapitein LC
J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174621 | pB80-empty | Empty backbone |
| 174622 | pB80-FKBP-mCherry-RAB11 | Rapalog-inducible coupling to recycling endosomes (RAB11) via FKBP |
| 174623 | pB80-iLID-mCherry-RAB11 | Optogenetic coupling to recycling endosomes (RAB11) via iLID |
| 174624 | pB80-iLID-mCherry-RAB5 | Optogenetic coupling to early endosomes (RAB5) via iLID |
| 174625 | pB80-LAMP1-mCherry-iLID | Optogenetic coupling to lysosomes (LAMP1) via iLID |
| 174626 | pB80-KIF1A(1-383)-GFP-SSPB(milli) | Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli) |
| 174627 | pB80-KIF1A(1-383)-GFP-SSPB(micro) | Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174628 | pB80-KIF1A(1-383)-GFP-SSPB(nano) | Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano) |
| 174629 | pB80-KIF1A(1-365)-GFP-SSPB(micro) | Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174630 | pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro) | Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174631 | pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro) | Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174632 | pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro) | Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174633 | pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) | Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174634 | pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) | Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174635 | pB80-KIF1A(1-365)-mVenus-SSPB(micro) | Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174636 | pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) | Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174637 | pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) | Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro) |
| 174638 | pB80-FRB-GFP-ppKin14VIb(861-1321) | Rapalog-inducible coupling to dimeric moss kinesin-14 for retrograde transport via FRB |
| 174639 | pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321) | Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro) |
| 174640 | pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321) | Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport |
| 174641 | pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321) | Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro) |
| 174642 | pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321) | Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport |
| 174643 | pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321) | Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport |
| 174644 | pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11 | Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes |
| 174645 | pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11 | Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes |
| 174646 | pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11 | Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system. |
| 174647 | pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11 | Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system. |
| 174648 | HA-BICD2(1-500)-FRB | Rapalog-inducible coupling to dynein adaptor BICD2 |