Diego Orzaez Lab:
Orzaez lab plasmids
Unpublished
Unpublished
Plasmids from Article
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160553 | Tobacco Etch Virus NIa protease (GB0420) | pUPD_NIaPro(TEV) |
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160554 | pUPD P35S (GB0552) | 35s promoter for N-terminal fusions |
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160555 | pUPD Pnos (GB0555) | Nos promoter for N-terminal fusions |
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160556 | pUPD DsRed-CT (GB1183) | DsRed for C-terminal fusions |
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160561 | pVD1 tRNA-gRNA position E2 (GB2239) | tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA |
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160562 | pVD1 tRNA-gRNA position E3 (GB2240) | tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter |
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160563 | pVD1 Multiplexing Edit E4 (GB2241) | tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA. |
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160564 | pVD1 Multiplexing Edit En-1 (GB2242) | tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter. |
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160565 | pVD1 tRNA-gRNA E4-En-1 (GB2243) | tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter. |
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160566 | pVD1 Multiplexing Edit E3-E4-En-1 (GB2244) | GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter. |
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160567 | pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245) | tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA |
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160569 | pUPD2 hpIntron (GB1281) | Intron sequence from S. lycopersicum |
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160584 | pUPD2 AlcA (GB1549) | Ethanol inducible operator |
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160585 | pUPD2 AlcR (GB1550) | Ethanol dependent transcrition factor |
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160587 | pUPD2 nickase D10A Cas9 (GB1690) | Nickase D10A Cas9 for Nt and Ct fusion. |
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160588 | pUPD2 nickase D10A Cas9 (GB1691) | Nickase D10A Cas9 for Nt fusion. |
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160589 | pUPD2 NtSPrbcS (GB2213) | B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain |
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160590 | pUPD2 GFP-NT (GB2236) | GFP for N-terminal fusions |
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160591 | pUPD2 GFP (GB2237) | GFP coding sequence. |
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160592 | pUPD2 GFP-CT (GB2238) | GFP for C-terminal fusions. |
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160595 | pUPD2_miniDFR (GB2566) | Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region |
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160596 | pUPD2 dCas9 (GB2664) | CDS of dCas9 with B3 nomenclature |
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160597 | pUPD2 MS2 (GB2665) | CDS of MS2 protein with B3 nomenclature |
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160598 | pUPD2 ER-ER (GB2666) | two repeats of ER (Estradiol receptor) |
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160599 | pUPD2 CUP2 (GB2716) | Copper dependent transcription factor |
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160600 | pUPD2 VP16 (GB2717) | Transcriptional activator |
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160601 | pUPD2 CBS4 (GB2718) | Copper dependent CUP2 binding DNA sequence |
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160602 | pUPD2 Etr4 (GB2719) |
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160603 | pUPD2 PhyB (GB2721) | chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch. |
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160605 | pUPD2 SV40-AcrIIA4 (GB3333) | SV40-AcrIIA4 codon optimized for N.benthamiana |
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160606 | pEGB3 alpha1 35s:PIF6:tNos (GB0170) | TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch |
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160607 | pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039) | TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription. |
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160608 | pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188) | TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools) |
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160612 | GB_PVX (GB1464) | PVX vector adapted to GB |
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160618 | pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656) | Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII). |
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160628 | pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234) | Module for the constitutive expression of the nptII, Cas9 and DsRed genes. |
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160629 | pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479) | TU for the expression of LbCas12a |
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160630 | pEGB3_alpha2 35:PhyB:VP16 (GB2745) | TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch |
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160631 | pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759) | Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter. |
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160632 | pEGB3_alpha1 Pnos:P19:Tnos (GB2762) | TU for expression of P19 driven by Nos promoter. |
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160634 | pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980) | TU for wtGFP expression driven by 2x35s promoter. |
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160635 | pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344) | TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator |
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160637 | pDGB3 alpha1 P35S:BFP:T35S (GB3416) | TU for the constitutive expression of Blue Fluorescent Protein |
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160638 | AtS/MAR10 insulator sequence (GB3458) | pEGB3a1 AtS/MAR10 |
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160639 | AtS/MAR10 insulator sequence (GB3459) | pEGB3a2 AtS/MAR10 |
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160640 | pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657) | Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII). |
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160646 | pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235) | Module for the constitutive expression of the nptII, Cas9 and DsRed genes. |
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160647 | pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469) | module containing the human codon optimized Cas9 and the BASTA selection marker |
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