Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a Genome Integrity Liability that Can Be Exploited by Targeting DNA Polymerase epsilon.
Sviderskiy VO, Blumenberg L, Gorodetsky E, Karakousi TR, Hirsh N, Alvarez SW, Terzi EM, Kaparos E, Whiten GC, Ssebyala S, Tonzi P, Mir H, Neel BG, Huang TT, Adams S, Ruggles KV, Possemato R
Mol Cell. 2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 10.1016/j.molcel.2020.10.016. PubMed Article
Sviderskiy VO, Blumenberg L, Gorodetsky E, Karakousi TR, Hirsh N, Alvarez SW, Terzi EM, Kaparos E, Whiten GC, Ssebyala S, Tonzi P, Mir H, Neel BG, Huang TT, Adams S, Ruggles KV, Possemato R
Mol Cell. 2020 Oct 28. pii: S1097-2765(20)30723-1. doi: 10.1016/j.molcel.2020.10.016. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160762 | pLKO.1P POLE1_1 | Suppress POLE1 |
| 160763 | pLKO.1B POLE1_1 | Suppress POLE1 |
| 160764 | pLKO.1P POLE1_2 | Suppress POLE1 |
| 160765 | pLKO.1B POLE1_2 | Suppress POLE1 |
| 160766 | pLKO.1P POLE2 | Suppress POLE2 |
| 160767 | pLKO.1B CDC25A_1 | Suppress CDC25A |
| 160768 | pLKO.1B CDC25A_2 | Suppress CDC25A |
| 160769 | pLKO.1B CDC25C_1 | Suppress CDC25C |
| 160770 | pLKO.1B CDC25C_2 | Suppress CDC25C |
| 160771 | pLKO.1P BRIP1_1 | Suppress BRIP1 |
| 160772 | pLKO.1P BRIP1_2 | Suppress BRIP1 |
| 160773 | pLKO.1P CISD1_1 | Suppress CISD1 |
| 160774 | pLKO.1P CISD1_2 | Suppress CISD1 |
| 160775 | pLKO.1P CISD2_1 | Suppress CISD2 |
| 160776 | pLKO.1P CISD2_2 | Suppress CISD2 |
| 160777 | pLKO.1P DDX11_1 | Suppress DDX11 |
| 160778 | pLKO.1P DDX11_2 | Suppress DDX11 |
| 160779 | pLKO.1P DNA2_1 | Suppress DNA2 |
| 160780 | pLKO.1P DNA2_2 | Suppress DNA2 |
| 160781 | pLKO.1P ERCC2_1 | Suppress ERCC2 |
| 160782 | pLKO.1P ERCC2_2 | Suppress ERCC2 |
| 160783 | pLKO.1P EXO5_1 | Suppress EXO5 |
| 160784 | pLKO.1P EXO5_2 | Suppress EXO5 |
| 160785 | pLKO.1P MUTYH_1 | Suppress MUTYH |
| 160786 | pLKO.1P MUTYH_2 | Suppress MUTYH |
| 160787 | pLKO.1P NTHL1_1 | Suppress NTHL1 |
| 160788 | pLKO.1P NTHL1_2 | Suppress NTHL1 |
| 160789 | pLKO.1P POLA1_1 | Suppress POLA1 |
| 160790 | pLKO.1P POLA1_2 | Suppress POLA1 |
| 160791 | pLKO.1P POLD1_1 | Suppress POLD1 |
| 160792 | pLKO.1P POLD1_2 | Suppress POLD1 |
| 160793 | pLKO.1P PPAT_1 | Suppress PPAT |
| 160794 | pLKO.1P PPAT_2 | Suppress PPAT |
| 160795 | pLKO.1P PRIM2_1 | Suppress PRIM2 |
| 160796 | pLKO.1P PRIM2_2 | Suppress PRIM2 |
| 160797 | pLKO.1P REV3L_1 | Suppress REV3L |
| 160798 | pLKO.1P REV3L_2 | Suppress REV3L |
| 160799 | pLKO.1P RTEL1_1 | Suppress RTEL1 |
| 160800 | pLKO.1P RTEL1_2 | Suppress RTEL1 |
| 160801 | pCW57.1 DOX off blast NFS1 | Express Dox repressible NFS1 |
| 160802 | pMXS-IRES-Blast POLE1 | Express POLE1 |
| 160803 | pMXS-IRES-Blast POLE1 N-ISCmut | Express N-terminal ISC mutant POLE1 |
| 160804 | pMXS-IRES-Blast POLE1 C-ISCmut | Express C-terminal ISC mutant POLE1 |
| 160805 | pMXS-IRES-Blast POLD1 | Express POLD1 |
| 160806 | pMXS-IRES-Blast POLD1 ISCmut | Express ISC mutant POLD1 |
| 160807 | pMXS-IRES-Blast POLA1 | Express POLA1 |
| 160808 | pMXS-IRES-Blast POLA1 ISCmut | Express ISC mutant POLA1 |
| 160809 | pMXS-IRES-Blast HA-POLE2 | Express HA-tagged POLE2 |
| 160810 | Tet-pLKO-puro shPOLE1_1 | Express Dox repressible shPOLE1 |