PAM-less plant genome editing using a CRISPR-SpRY toolbox.
Ren Q, Sretenovic S, Liu S, Tang X, Huang L, He Y, Liu L, Guo Y, Zhong Z, Liu G, Cheng Y, Zheng X, Pan C, Yin D, Zhang Y, Li W, Qi L, Li C, Qi Y, Zhang Y
Nat Plants. 2021 Jan 4. pii: 10.1038/s41477-020-00827-4. doi: 10.1038/s41477-020-00827-4. PubMed Article
Ren Q, Sretenovic S, Liu S, Tang X, Huang L, He Y, Liu L, Guo Y, Zhong Z, Liu G, Cheng Y, Zheng X, Pan C, Yin D, Zhang Y, Li W, Qi L, Li C, Qi Y, Zhang Y
Nat Plants. 2021 Jan 4. pii: 10.1038/s41477-020-00827-4. doi: 10.1038/s41477-020-00827-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161520 | pYPQ166-SpRY | Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter |
| 161521 | pYPQ266E | Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing |
| 161522 | pYPQ262m | Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing |
| 161523 | pYPQ262-ABE8e | Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing |
| 161524 | pYPQ262B-ABE8e | Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing |