Efficient C*G-to-G*C base editors developed using CRISPRi screens, target-library analysis, and machine learning.
Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR
Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 10.1038/s41587-021-00938-z. PubMed Article
Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR
Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 10.1038/s41587-021-00938-z. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163526 | pCMV_Anc689-nCas9 | Mammalian CG-to-GC base editing |
| 163527 | pCMV_Anc689-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163531 | pCMV_APOBEC1-UdgX-nCas9 | Mammalian CG-to-GC base editing |
| 163533 | pCMV_APOBEC1-UdgX-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163535 | pCMV_eA3A-nCas9 | Mammalian CG-to-GC base editing |
| 163536 | pCMV_eA3A-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163537 | pCMV_eA3A T31A-dCas9 | Mammalian CG-to-GC base editing |
| 163538 | pCMV_eA3A T31A-nCas9 | Mammalian CG-to-GC base editing |
| 163539 | pCMV_eA3A T31A-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163541 | pCMV_EE-nCas9 | Mammalian CG-to-GC base editing |
| 163542 | pCMV_EE-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163543 | pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX | Mammalian CG-to-GC base editing |
| 163545 | pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX | Mammalian CG-to-GC base editing |
| 163546 | pCMV_RBMX-eA3A-UdgX-nCas9 | Mammalian CG-to-GC base editing |
| 163548 | pCMV_RBMX-eA3A-UdgX-HF-nCas9 | Mammalian CG-to-GC base editing |
| 163550 | pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163551 | pCMV_RBMX-eA3A-UdgX-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163552 | pCMV_UdgX-Anc689-UdgX-nCas9-RBMX | Mammalian CG-to-GC base editing |
| 163554 | pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX | Mammalian CG-to-GC base editing |
| 163555 | pCMV_UdgX-APOBEC1-UdgX-nCas9 | Mammalian CG-to-GC base editing |
| 163559 | pCMV_UdgX-APOBEC1-UdgX-HF-nCas9 | Mammalian CG-to-GC base editing |
| 163561 | pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163562 | pCMV_UdgX-APOBEC1-UdgX-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163564 | pCMV_UdgX-EE-UdgX-nCas9 | Mammalian CG-to-GC base editing |
| 163565 | pCMV_UdgX-EE-UdgX-nCas9-RBMX | Mammalian CG-to-GC base editing |
| 163566 | pCMV_UdgX-EE-UdgX-nCas9-UdgX | Mammalian CG-to-GC base editing |
| 163570 | pCMV_UdgX-EE-UdgX-NG-nCas9 | Mammalian CG-to-GC base editing |
| 163571 | pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX | Mammalian CG-to-GC base editing |
| 163572 | pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX | Mammalian CG-to-GC base editing |