Mario Capecchi Lab:
Additional gene targeting vector construction plasmids
Nam HS, Capecchi MR
Unpublished
Nam HS, Capecchi MR
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128845 | pDTA-5 | Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number |
| 134322 | rox Neo* rox | rox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette |
| 138412 | attB Neo* attP | attB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassette |
| 138413 | PB Neo* PB | Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette |
| 140235 | F3 Neo* F3 | F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette |
| 168769 | lox2272 Neo* lox2272 | lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette |
| 176504 | pBS-2xpA | 2x SV40 polyA sequences |
| 176505 | pBS-3xpA | 3x SV40 polyA sequences |
| 176506 | pBS-4xpA | 4x SV40 polyA sequences |
| 178546 | H2B-sfGFP-T2A-iCre | pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT |
| 182338 | pMOD4-mT2A-mutated_rpsL-BSD-F | Template plasmid for PCR amplification of initial recombineering cassette |
| 182339 | mT2A-td-sfGFP-FNF | Recombineering cassette plasmid for T2A-td-sfGFP-FNF |
| 182340 | mT2A-tdTomato-FNF | Recombineering cassette plasmid for T2A-tdTomato-FNF |
| 182341 | mT2A-sfGFP-linker-iCre-ERT2-FNF | Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF |
| 182342 | mT2A-TagRFP-T-linker-dre-PBD*-FNF | Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF |
| 182363 | miR-124 | Plasmid with 4x miR-124 target sites |
| 188230 | IRES-creERT2-FNF | EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette |