Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth.
Perez-Leal O, Nixon-Abell J, Barrero CA, Gordon JC, Oesterling J, Rico MC
CRISPR J. 2021 Nov 30. doi: 10.1089/crispr.2021.0041. PubMed Article
Perez-Leal O, Nixon-Abell J, Barrero CA, Gordon JC, Oesterling J, Rico MC
CRISPR J. 2021 Nov 30. doi: 10.1089/crispr.2021.0041. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167205 | FASTHDR-Cterm-mClover3-Puromycin | Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin |
| 167206 | FASTHDR-Cterm-mRuby3-Zeo | Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin |
| 167207 | FASTHDR-Cterm-mTagBFP2-Blasticidin | Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin |
| 167208 | FASTHDR-Cterm-HaloTag-Puromycin | Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin |
| 167209 | FASTHDR-Cterm-NanoLuc-Puromycin | Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin |
| 167210 | FASTHDR-tRNApromo-sgRNA-espCas91_1 | Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1) |