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Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson's disease-linked DNAJC6 mutations.
Wulansari N, Darsono WHW, Woo HJ, Chang MY, Kim J, Bae EJ, Sun W, Lee JH, Cho IJ, Shin H, Lee SJ, Lee SH
Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb1540. doi: 10.1126/sciadv.abb1540. Print 2021 Feb.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
170443pCDH-EF1a-hDNAJC6-T2A-copGFPLentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter
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170444pCDH-EF1a-hLMX1A-T2A-copGFPLentiviral vector expressing human LMX1A (with silent mutation) with copGFP reporter gene, under control of EF1alpha promoter.
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170445pCDH-EF1a-hSNCALentiviral vector expressing human SNCA, under control of EF1alpha promoter.
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170446pCDH-EF1a-mCherry-EGFP-LC3BLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.
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Antibodies from Article