Reconstitution of purified membrane protein dimers in lipid nanodiscs with defined stoichiometry and orientation using a split GFP tether.
Bruguera ES, Mahoney JP, Weis WI
J Biol Chem. 2022 Jan 21:101628. doi: 10.1016/j.jbc.2022.101628. PubMed Article
Bruguera ES, Mahoney JP, Weis WI
J Biol Chem. 2022 Jan 21:101628. doi: 10.1016/j.jbc.2022.101628. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182931 | FLAG-3C-sGFP1-10_pcDNA3.1(-) | For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment |
| 182932 | FLAG-3C-sGFP11_pcDNA3.1(-) | For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment |