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Generation of stable Drosophila cell lines using multicistronic vectors.
Gonzalez M, Martin-Ruiz I, Jimenez S, Pirone L, Barrio R, Sutherland JD
Sci Rep. 2011;1:75. doi: 10.1038/srep00075. Epub 2011 Aug 31. PubMed Article

Plasmids from Article

ID Plasmid Purpose
32425Ac5-STABLE1-neo
32426Ac5-STABLE2-neo
208031Ac5-STABLE1-puroEnables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.
208032Ac5-STABLE2-puroEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.
208033Ac5-STABLE1-blastEnables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.
208034Ac5-STABLE2-blastEnables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

Antibodies from Article