A versatile and efficient high-throughput cloning tool for structural biology.
Geertsma ER, Dutzler R
Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. PubMed Article
Geertsma ER, Dutzler R
Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 46858 | pINIT_cat | FX sequencing vector for subcloning, chloramphenicol marker |
| 46973 | pINIT_kan | FX sequencing vector for subcloning, kanamycin marker |
| 46974 | pINIT_tet | FX sequencing vector for subcloning, tetracycline marker |
| 47064 | p7XNH3 | FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site |
| 47065 | p7XC3H | FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag |
| 47066 | p7XC3GH | FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags |
| 47067 | pBXNH3 | FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site |
| 47068 | pBXC3H | FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag |
| 47069 | pBXNH3CA | FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag |
| 47070 | pBXC3GH | FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags |
| 47071 | pBXCA3GH | FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags |
| 47072 | pBXCA3H | FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag |
| 47073 | pBXNAC3GH | FX cloning E.coli expression vector with araBAD promoter, N-terminal Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags |
| 47074 | pBXNAC3H | FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag |
| 47075 | pBXNH3A | FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tag |
| 47077 | pREXC3GH | FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags |
| 47078 | pREXC3H | FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tags |
| 47079 | pREXNH3 | FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage site |
| 49020 | pICXNH3 | FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site |
| 49021 | pICXNHG3 | FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site |
| 49022 | pICXC3H | FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag |
| 49023 | pICXC3GH | FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag |
| 49024 | pYEXNH3 | FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site |
| 49025 | pYEXNHG3 | FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site |
| 49026 | pYEXC3H | FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag |
| 49027 | pYEXC3GH | FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag |
| 49028 | pcDXNSM3 | FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site |
| 49029 | pcDXNSMG3 | FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site |
| 49030 | pcDXC3MS | FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide |
| 49031 | pcDXC3GMS | FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide |
| 49032 | pTLNX | FX cloning Xenopus oocyte expression vector with SP6 promoter |
| 49033 | pTLNXA7 | FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence |