A new method to customize protein expression vectors for fast, efficient and background free parallel cloning.
Scholz J, Besir H, Strasser C, Suppmann S
BMC Biotechnol. 2013 Feb 14;13(1):12. PubMed Article
Scholz J, Besir H, Strasser C, Suppmann S
BMC Biotechnol. 2013 Feb 14;13(1):12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 43974 | pCoofy1 | |
| 43975 | pCoofy18 | |
| 43976 | pCoofy7 | |
| 43977 | pCoofy12 | |
| 43978 | pCoofy34 | |
| 43979 | pCoofy21 | |
| 43980 | pCoofy19 | |
| 43981 | pCoofy2 | |
| 43982 | pCoofy38 | |
| 43983 | pCoofy3 | |
| 43984 | pCoofy8 | |
| 43985 | pCoofy35 | |
| 43986 | pCoofy4 | |
| 43987 | pCoofy15 | |
| 43988 | pCoofy16 | |
| 43989 | pCoofy5 | |
| 43990 | pCoofy6 | |
| 43991 | pCoofy17 | |
| 43992 | pCoofy22 | |
| 43993 | pCoofy31 | |
| 43994 | pCoofy36 | |
| 43995 | pCoofy24 | |
| 43996 | pCoofy23 | |
| 43997 | pCoofy25 | |
| 43998 | pCoofy37 | |
| 44000 | pCoofy26 | |
| 44001 | pCoofy33 | |
| 44002 | pCoofy11 | |
| 44003 | pCoofy27 | |
| 44004 | pCoofy28 | |
| 44005 | pCoofy29 | |
| 44006 | pCoofy40 | |
| 55184 | pCoofy41 | Insect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC. |
| 55185 | pCoofy44 | Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC. |
| 55186 | pCoofy48 | Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC. |
| 55187 | pCoofy49 | Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and MBP tags. C-terminal tags can be added during SLIC. |
| 55188 | pCoofy47 | Mammalian expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC. |
| 55189 | pCoofy50 | Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC. |
| 55190 | pCoofy42 | Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC. |
| 122004 | pCoofy9 | E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site |
| 122005 | pCoofy51 | Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site |
| 122006 | pCoofy52 | E.coli vector for parallel SLIC cloning containing N-terminal His6-3xAvi with PreScission Cleavage site |
| 122007 | pCoofy55 | Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options |
| 122008 | pCoofy56 | Mammalian vector for parallel SLIC cloning containing N-terminal TwinStrep and PreScission Cleavage site |
| 122009 | pCoofy57 | Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options |
| 122011 | pCoofy59 | Baculovirus vector for parallel SLIC cloning containing N-terminal TrxA-His6 with PreScission Cleavage site |
| 122012 | pCoofy60 | Baculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site |
| 122013 | pCoofy61 | E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site |
| 122014 | pCoofy62 | Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options |
| 122015 | pCoofy63 | Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options |
| 122016 | pCoofy64 | Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options |
| 125176 | pCoofy65 | Mammalian vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options |