High-yield intra- and extracellular protein production using Bacillus megaterium.
Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara E, Jahn D
Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30. PubMed Article
Stammen S, Muller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara E, Jahn D
Appl Environ Microbiol. 2010 Jun;76(12):4037-46. doi: 10.1128/AEM.00431-10. Epub 2010 Apr 30. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48118 | pSPYngK-hp | Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter |
| 48119 | pSPVpr-hp | Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoter |
| 48120 | pSPNprM-hp | Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoter |
| 48121 | pSPYngK+-hp | Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter |
| 48122 | pSPAsp-hp | Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter |
| 48123 | pSPPac-hp | Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Pac under control of the xylose inducible promoter |