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Protein ligation in living cells using sortase.
Strijbis K, Spooner E, Ploegh HL
Traffic. 2012 Jun;13(6):780-9. doi: 10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
50029pRS303-pGAL-HA-SrtAstrep (pKS78)Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast
50030pcDNA3.1(-)-HA-SrtAstrep (pKS77)Expression of cytosolic S. pyogenes SrtA in mammalian cells
50031pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells
50032pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast
50033pRS303-pGAL-HA-TMSec66-SrtA (pKS105)Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast

Antibodies from Article