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mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin.
Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, Turk BE, Yaffe MB, Sabatini DM
Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48799pLJM60 mUlk1 (wt) barcodedexpresses wt Ulk1
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48800pLJM60-S6K1(T389S) barcodedexpresses mutant S6K1
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48801pLJM60-S6K1(wt) barcodedexpresses wt S6K1
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48805pRK5 HA-GST-Presc-mAkt1 (wt)expresses wt Akt1
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48806pRK5 HA-GST-Presc-mAkt1 (S473T)expresses mutant Akt1
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Antibodies from Article