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Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors.
Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
Curr Biol. 2003 Jan 8;13(1):41-6. PubMed Article

Plasmids from Article

ID Plasmid Purpose
50353pBluescript-mouse eIF2C1Mouse eIF2C1 cDNA clone
50354pBluescript-mouse eIF2C2Mouse eIF2C2 cDNA clone
50355pBluescript-mouse eIF2C3Mouse eIF2C3 cDNA clone
50356pBluescript-mouse eIF2C4Mouse eIF2C4 cDNA clone
50357pBluescript-mouse DicerMouse Dicer cDNA clone
50360pcDNAM-human eIF2C1-WT-MycExpression of human eIF2C1 in mamalian cells
50361pcDNAM-human eIF2C1-A-MycExpression of human eIF2C1 mutant in mamalian cells
50362pcDNAM-human eIF2C1-B-MycExpression of human eIF2C1 mutant in mamalian cells

Antibodies from Article