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Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing.
Gao Y, Zhao Y
J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51055pACT2-CAS9cExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
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51056pRS316-RGR-GFPExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
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51057pRS316-RGR-GFP-mHHSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.
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51058pRS316-RGR-GFP-mHDVSame as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.
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51059pRS316-RGR-GFP-mmSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.
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Antibodies from Article