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Sema4D localizes to synapses and regulates GABAergic synapse development as a membrane-bound molecule in the mammalian hippocampus.
Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S
Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51597pSuper 44-1shRNA 1 against rat and mouse Sema4D
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51598pSuper 44-2shRNA 2 against rat Sema4D
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51599pCMV-myc-Sema4Dexpresses mouse Sema4D with N-terminal myc tag
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51600pCMV-myc-Sema4D RNAiRexpresses RNAi-resistant mouse Sema4D with N-terminal myc tag
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51601pCMV-myc-Sema4D-CD4-FLAGexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4
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51602pCMV-myc-Sema4DdeltaCexpresses Sema4D lacking C terminus
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51603pCMV-myc-CD4-Sema4Dexpresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4
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51604pCMV-CD4-FLAGexpresses CD4 with C-terminal FLAG tag
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51606FRIG-myc-Sema4D-CD4expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
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51607FRIG-myc-Sema4DdeltaCexpresses myc-tagged Sema4D lacking C terminus in lentiviral backbone
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Antibodies from Article