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A Bicistronic MAVS Transcript Highlights a Class of Truncated Variants in Antiviral Immunity.
Brubaker SW, Gauthier AE, Mills EW, Ingolia NT, Kagan JC
Cell. 2014 Feb 13;156(4):800-11. doi: 10.1016/j.cell.2014.01.021.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51999pcDNA3-HA-MAVS-M142A(KaganF61)Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"
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52000pcDNA3-MAVS(KaganF57)Expresses the human MAVS CDS containing "weak translational context"
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52001pcDNA3-MAVS-M1A(KaganF58)Expresses the human MAVS CDS containing the point mutation M1A
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52002pcDNA3-MAVS-M1A,M142A(KaganF62)Expresses the human MAVS CDS containing two point mutations M1A and M142A
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52003pcDNA3-MAVS-M1A500(KaganF60)Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain
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52004pMSCV-HA-shift-IRES-GFP(KaganE21)Expresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP marker
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52005pcDNA3-5'UTR-MAVS(KaganD88)Expresses the human MAVS CDS and contains the 5'UTR sequense
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52006pcDNA3-5'UTR-MAVS-ORF1(KaganE49)Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon
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52007pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon
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52008pMSCV-MAVS(KaganE17)Expresses the human MAVS CDS
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52009pMSCV-MAVS-M1A(KaganE18)Expresses the human MAVS CDS containing the point mutation M1A
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52010pMSCV-MAVS-M142A(KaganE19)Expresses the human MAVS CDS containing the point mutation M142A
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52011pMSCV-MAVS-M1A,M142A(KaganE20)Expresses the human MAVS CDS containing the point mutations M1A and M142A
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52012pcDNA3-MAVS-invitro(KaganD86)Used for in vitro expression of the human MAVS CDS
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52013pcDNA3-MAVS-L62M-invitro(KaganD98)Used for in vitro expression of the human MAVS CDS containing a point mutation L62M
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52014pcDNA3-MAVS-G67M-invitro(KaganD100)Used for in vitro expression of the human MAVS CDS containing a point mutation G67M
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52015pcDNA3-MAVS-E80M-invitro(KaganD99)Used for in vitro expression of the human MAVS CDS containing a point mutation E80M
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52016pcDNA3-MAVS-M1A-invitro(KaganE12)Used for in vitro expression of the human MAVS CDS containing a point mutation M1A
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52017pcDNA3-MAVS-M142A-invitro(E13)Used for in vitro expression of the human MAVS CDS containing a point mutation M142A
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52018pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A
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52019p3XFlag-CMV7-miniMAVS(KaganE48)Expresses miniMAVS (the sequence starting from M142 of the MAVS CDS) with an N-terminal 3X flag tag
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52062pcDNA3-MAVS-M142A(KaganF59)Expresses the human MAVS CDS containing the point mutation M142A "weak translational context"
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Antibodies from Article