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Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC
Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51129pST1374-N-NLS-flag-linker-Cas9-H840ACas9 nickase optimized for nuclear import.
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51130pST1374-N-NLS-flag-linker-Cas9-D10ACas9 nickase optimized for nuclear import.
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51132pUC57-sgRNA expression vectorFor in vitro transcription of sgRNA from the T7 promoter.
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51133pGL3-U6-sgRNA-PGK-puromycinexpresses sgRNA from the U6 promoter.
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Antibodies from Article