Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Cost-benefit tradeoffs in engineered lac operons.
Eames M, Kortemme T
Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52695delta-ZYAMG1655 with Lac Operon removed from native locus
Loading...
52696KIlacMG1655 with Lac Operon removed from native locus and re-integrated at the attTn7 locus
Loading...
52697OpLac1KIlac with a codon optimized lacZ using preferred codon at every position
Loading...
52698OpLac1-delta-6OpLac1 without the first 6 nucleotides of lacZ
Loading...
52699OpLac2KIlac with a codon optimized lacZ using codons for a subset of amino acids read by transfer RNAs that are the most highly charged during amino acid starvation
Loading...
52700OpLac2-delta-6OpLac2 without the first 6 nucleotides of lacZ
Loading...
52701Oplac1 (37)-KIKIlac with first 37 bases from Oplac1
Loading...
52702Oplac2 (37)-KIKIlac with first 37 bases from Oplac2
Loading...
52703KI (37)-Oplac1Oplac1 with first 37 bases from KIlac
Loading...
52704KI (37)-Oplac2Oplac2 with first 37 bases from KIlac
Loading...
52705degtagKIlac with lacZ C-terminal sssA degradation tag
Loading...
52706delta-ZKIlac with lacZ deletion
Loading...
52707delta-ZAKIlac with lacZ & lacA deletion
Loading...
52708delta-YAKIlac with lacY and lacA deletion
Loading...
52709S56LKIlac with S56L mutation in lacY (impairs function)
Loading...
52710G794DKIlac with G794D mutation in lacZ (impairs function)
Loading...
52711G794AKIlac with G794A mutation in lacZ (impairs function)
Loading...
52712W999FKIlac with W999F mutation in lacZ (impairs function)
Loading...
52713W999LKIlac with W999L mutation in lacZ (impairs function)
Loading...
52714E537QKIlac with E537Q mutation in lacZ (impairs function)
Loading...

Antibodies from Article