Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and protein biosynthesis.
Huot G, Vernier M, Bourdeau V, Doucet L, Saint-Germain E, Gaumont-Leclerc MF, Moro A, Ferbeyre G
Mol Biol Cell. 2014 Mar;25(5):554-65. doi: 10.1091/mbc.E13-02-0110. Epub 2014 Jan 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
51836pLPC-Flag-CHES1Lencodes the long isoform of human CHES1 and a Flag tag
51837pLPC-Flag-CHES11L-dFCFlag-CHES1L mutant lacking the forkhead binding site and the C-terminal domains
51838pLPC-Flag-CHES1LdFC-NLSFlag-CHES1L mutant lacking the forkhead binding site and C-terminal domain, with remaining NLS
51839pLPC-Flag-CHES1L-dCFlag-CHES1L mutant lacking the C-terminal and NLS domains
51840pLPC-Flag-CHES1L-dC-NLSFlag-CHES1L mutant lacking the C-terminal, with remaining NLS
51841pLPC-Flag-CHES1L-DBD-NLSFlag-CHES1L mutant encoding the DNA binding and NLS domains only
51842pLPC-Flag-CHES1L-dFNFlag-CHES1L mutant lacking the forkhead binding site and N-terminal domains
51843pLPC-Flag-CHES1L-dNFlag-CHES1L mutant lacking the N-terminal domain
51844pLPC-Flag-CHES1H164AFlag-CHES1L mutant with ponctual mutation on histidine 164
51845mlp-hCHES1shshRNA against CHES1
51846pBabe-CHES1-ERfusion protein between CHES1 and the ligand binding domain of estrogen receptor
51847pWZL-rCHES1encodes a CHES1 mRNA that is resistant to shRNA against CHES1
51848pGL3-PIM2promoter region (-550;0) of PIM2
51849pGL3-PIM2mut1promoter region of PIM2 lacking the forkhead binding site #2
51850pGL3-PIM2mut2promoter region of PIM2 lacking the forkhead binding site #1
53742pGL3-PIM2mut3promoter region of PIM2 lacking the 2 forkhead binding sites
53743pLPC-CHES1Llong isoform of human CHES1

Antibodies from Article