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Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR.
Fujita T, Fujii H
PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
51128pSIR-neoSelf-inactivating retrovirus vector containing a neomycin-resistance gene
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51134pSIR-GFPSelf-inactivating retrovirus vector containing the GFP gene
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51135pSIR-DsRed-Express2Self-inactivating retrovirus vector containing the DsRed-Express2 gene
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51143pSIR-hCD2Self-inactivating retrovirus vector containing the human CD2 gene
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512403xFLAG-dCas9/pMXs-puroExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
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512583xFLAG-dCas9/pMXs-IGExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
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512593xFLAG-dCas9/pMXs-I2Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
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512603xFLAG-dCas9/pMXs-neoExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
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61080gRNA-hIRF-1 #12/pSIRExpresses a guide RNA (gRNA) to target human IRF-1 promoter
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