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"Measuring fast gene dynamics in single cells with timelapse luminescence microscopy"
Mazo-Vargas A, Park H, Aydin M, Buchler NE
Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60114pNB774Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)
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60115pNB775Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)
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60117pNB776Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)
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60119pNB687Yellow beetle luciferase
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60133pNB689Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
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60134pNB690Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
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60135pNB691Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
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60136pNB649Firefly luciferase (Promega) driven by MET17 promoter.
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60138pNB769Red click beetle luciferase from Promega driven by MET17 promoter.
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60139pNB768Green click beetle luciferase from Promega driven by MET17 promoter.
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60140pNB648NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.
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60142pNB655Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.
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60143pNB682Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.
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60144pNB695Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
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60145pNB693Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
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60146pNB697Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
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60147pNB330Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)
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60148pNB753Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.
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60149pNB756Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.
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60150pNB762Firefly luciferase (Promega) driven by LEU1 promoter.
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60151pNB786Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.
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60152pNB787Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
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60153pNB777Red click beetle luciferase (Promega) driven by LEU1 promoter.
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60154pNB778Green click beetle luciferase (Promega) driven by LEU1 promoter.
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60155pNB752Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.
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60156pNB761Firefly luciferase (Promega) driven by ADE17 promoter.
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60157pNB754Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.
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60158pNB763Firefly luciferase (Promega) driven by LYS9 promoter.
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60159pNB767Firefly luciferase (Promega) driven by SIC1 promoter.
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60160pNB812Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.
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60161pNB813Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.
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60162pNB783Firefly luciferase (Promega) driven by RNR1 promoter.
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60163pNB784Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.
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60164pNB785Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
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60165pNB788Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.
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60166pNB789Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
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Antibodies from Article