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A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila.
Zhang X, Koolhaas WH, Schnorrer F
G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. PubMed Article

Plasmids from Article

ID Plasmid Purpose
60944pJET1.2-STOP-dsRedDrosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.
60945pBS-GGAC-TTCTDrosophila, backbone for donor plasmid assembly. CRISPR knock-in.
60948p3xP3-EGFP.vas-int.NLSDrosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.
60949pBS-GGAC-ATGCDrosophila, backbone for donor plasmid assembly. CRISPR knock-in.
60950pBS-CGGA-GTGCDrosophila, backbone for donor plasmid assembly. CRISPR knock-in.
61255pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette
61256pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette
61257pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

Antibodies from Article