Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.
Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F
Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31. PubMed Article
Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F
Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61051 | DR274-eGFP sgRNA | for generation of a eGFP specific sgRNA from a T7 promoter |
| 61069 | eGFPbait-E2A-KalTA4-pA donor vector | for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051) |