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A set of fluorescent protein-based markers expressed from constitutive and arbuscular mycorrhiza-inducible promoters to label organelles, membranes and cytoskeletal elements in Medicago truncatula.
Ivanov S, Harrison MJ
Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706. PubMed Article

Plasmids from Article

ID Plasmid Purpose
61168pCMU-NUCrFluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter
61169pCMB-NUCrFluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter
61170pCMU-ERrFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under AtUBQ10 promoter
61171pCMB-ERrFluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under MtBCP1 promoter
61172pCMU-GArFluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter
61173pCMB-GArFluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under MtBCP1 promoter
61175pCMB-TGN61rFluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter
61176pCMU-TGN41rFluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoter
61177pCMB-TGN41rFluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under MtBCP1 promoter
61178pCMU-TGNVHArFluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter
61179pCMB-TGNVHArFluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under MtBCP1 promoter
61180pCMU-PMrFluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter
61181pCMB-PMrFluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter
61182pCMU-APrFluorescent reporter for apoplast MtBCP1sp-mCherry expressed under AtUBQ10 promoter
61183pCMB-APrFluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtBCP1 promoter
61184pCMP-APrFluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtPT4 promoter
61185pCMU-LErFluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoter
61186pCMB-LErFluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoter
61187pCMU-TLErFluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter
61188pCMB-TLErFluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter
61189pCMU-TPrFluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter
61190pCMB-TPrFluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter
61191pCMU-ACTFrFluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoter
61192pCMB-ACTFrFluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter
61193pCMU-ACTLrFluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter
61194pCMB-ACTLrFluorescent reporter for F-actin LifeAct-mCherry expressed under MtBCP1 promoter
61195pCMB-ACTLgFluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter
61196pCMU-MTUBrFluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoter
61197pCMB-MTUBrFluorescent reporter for microtubules mCherry-MAP4-MBD expressed under MtBCP1 promoter
61198pCMU-MITrFluorescent reporter for motochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter
61199pCMB-MITrFluorescent reporter for motochondria ScCOX4sp-mCherry expressed under MtBCP1 promoter
61200pCMU-PLArFluorescent reporter for plastids MtRubisco1sp-mCherry expressed under AtUBQ10 promoter
61201pCMB-PLArFluorescent reporter for plastids MtRubisco1sp-mCherry expressed under MtBCP1 promoter
61202pCMU-PERrFluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter
61203pCMB-PERrFluorescent reporter for peroxisomes mCherry-SKL expressed under MtBCP1 promoter
61204pCMU-AUTrFluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter
61205pCMB-AUTrFluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter
61206pCMU-PDESrFluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter
61207pCMB-PDESrFluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter
61208pCMB-TMErFluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter
61209pCMB-TMEgFluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

Antibodies from Article