Directed evolution of APEX2 for electron microscopy and proximity labeling.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY
Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. PubMed Article
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY
Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49385 | pcDNA3 Connexin43-GFP-APEX2 | Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells |
| 49386 | pcDNA3 APEX2-NES | Expresses APEX2 in the cytosol of mammalian cells |
| 66170 | Vimentin - APEX2 in pECFP | APEX2 fused to the C-terminus human vimentin |
| 66171 | APEX2 - Tubulin in pEGFP | APEX2 fused to the N-terminus of alpha tubulin |
| 66172 | APEX2 - Actin in pEGFP | APEX2 fused to the N-terminus of human beta actin |
| 72480 | mito-V5-APEX2 | Expresses APEX2 in mitochondria |
| 72558 | pTRC-APEX2 | Expression of cytosolic APEX2 in bacteria |
| 79055 | ERM-APEX2 | Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1. |
| 79056 | APEX2-OMM | Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein |
| 79057 | MICU-APEX2 | A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1 |