CRISPR/Cas9 Plasmids and Resources

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Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use the CRISPR/Cas9 technology in your own lab. Browse plasmids below or check out our CRISPR resources on how to start using CRISPR in your lab.

Addgene CRISPR Resources

• CRISPR Guide: Essential background information on CRISPR/Cas9 and the basics for planning your first CRISPR experiment.
• CRISPR Resources: Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and more.
• CRISPR History: Learn how CRISPR/Cas9 was modified from a bacterial immune system to a revolutionary tool for genome engineering.
• Blog Posts: Want the latest news on CRISPR? Experts cover CRISPR/Cas9 topics on Addgene's blog.

Browse CRISPR Plasmids by Function

	Cut	Wild type Cas9 efficiently generates double strand breaks (DSBs) at sequences homologous to co-expressed gRNA.
	Nick	A mutated "nickase" version of the Cas9 enzyme generates a single-strand DNA break (Nick), instead of a double-strand DNA break (Cut).
	Interfere	A catalytically inactive Cas9 (dCas9) can knockdown gene expression by interfering with transcription. The dCas9 can sometimes be fused to an additional repressor peptide.
	Activate	A catalytically inactive Cas9 (dCas9) fused to an activator peptide can activate or increase gene expression.
	dCas9-FokI	A catalytically inactive Cas9 (dCas9) fused to FokI nuclease to generate double strand breaks (DSBs, Cut) at sequences homologous to two co-expressed gRNA.
	Purify	Isolate specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused with an epitope tag(s).
	Visualize	Visualize specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused to a fluorescent protein.
	Screen	Use pooled CRISPR libraries to screen for genes involved in specific biological processes.
	Tag	Find the tools for tagging your endogenous protein of interest.
	Validated gRNAs	Experimentally validated, pre-made gRNA plasmids to specific genes.
	Empty gRNA Vectors	Select a gRNA plasmid based on a variety of factors, such as presence or absence of Cas9 and number of gRNAs (multiplex vectors).

Browse Plasmids by Model Organism

Mammalian	Bacteria	Drosophila	Plant
C. elegans	Yeast	Zebrafish	Xenopus