| hCas9 | Cas9 | CMV | |
Church
| RNA-Guided Human Genome Engineering via Cas9.
Science. 2013 Jan 3.
|
Expresses human codon optimized Cas9 nuclease for genome engineering
pcDNA3.3-TOPO
|
| pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro | humanized S. pyogenes Cas9 | | Puromycin |
Zhang
| Multiplex Genome Engineering Using CRISPR/Cas Systems.
Science. 2013 Jan 3.
|
This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.
pUC ori vector
|
| pX330-U6-Chimeric_BB-CBh-hSpCas9 | humanized S. pyogenes Cas9 | CBh | |
Zhang
| Multiplex Genome Engineering Using CRISPR/Cas Systems.
Science. 2013 Jan 3.
|
A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.
pUC ori vector
Tag
/ Fusion Protein- 3xFLAG (N terminal on insert)
|
| pMJ920 | Cas9 (Synthetic) | CMV | |
Doudna
| RNA-programmed genome editing in human cells.
elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29.
|
MacroLab 6D
|
| JDS246 | mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag | CMV | |
Joung
|
Joung lab unpublished CRISPR plasmids
(unpublished)
|
Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
unknown
|
| p3s-Cas9HC | Cas9 | CMV | |
Kim
| Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507.
|
pCDNA3.1
|
| pCas9_GFP | Cas9-2A-GFP (Synthetic) | CAG | |
Musunuru
| Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs.
Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 10.1016/j.stem.2013.03.006.
|
Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
pCAG
|
| pST1374-NLS-flag-linker-Cas9 | Human codon optimized Cas9 | CMV | Blasticidin |
Huang
| Generation of gene-modified mice via Cas9/RNA-mediated gene targeting.
Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46.
|
pST1374 (Addgene #13426)
|
| pSimpleII-NLS-NmCas9-HA-NLS(s) | NmCas9 (Other) | EF1a | |
Thomson
| Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis.
Proc Natl Acad Sci U S A. 2013 Aug 12.
|
This plasmid encode NmCas9 with two NLS and a HA tag
pSimpleII
|
| pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) | NmCas9 (Other), U6pr-tracrRNA (Other) | EF1a, U6 | |
Thomson
| Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis.
Proc Natl Acad Sci U S A. 2013 Aug 12.
|
This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
pSimpleII
|
| pSimpleII-NmCas9-FLAG | NmCas9 (Other) | | |
Thomson
| Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis.
Proc Natl Acad Sci U S A. 2013 Aug 12.
|
FLAG tagged NmCas9 with no NLS.
pSimpleII
|
| pSpCas9 (PX165) | hSpCas9 (Synthetic) | Cbh | |
Zhang
| Genome engineering using the CRISPR-Cas9 system.
Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
|
Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)
PX165
|
| pSpCas9(BB)-2A-GFP (PX458) | hSpCas9 (Synthetic) | Cbh | |
Zhang
| Genome engineering using the CRISPR-Cas9 system.
Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
|
Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA
PX458
|
| pSpCas9(BB)-2A-Puro (PX459) | hSpCas9-2A-Puro (Synthetic) | Cbh | Puromycin |
Zhang
| Genome engineering using the CRISPR-Cas9 system.
Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
|
NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.
PX459
|
| pCAG-T3-hCAS-pA | Codon optimized Cas9 (Synthetic) | CAG promoter | |
Fujii
| Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease.
Nucleic Acids Res. 2013 Aug 30.
|
Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys
pCAGGS
|
| M-SPcas | Cas9 (Other) | CMV | Neomycin (select with G418) |
Church
| Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
|
Mammalian S. pyogenes Cas9 expression, human optimized
pcDNA3.3 TOPO
|
| M-ST1cas | Cas9 (Other) | CMV | Neomycin (select with G418) |
Church
| Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
|
Mammalian S. thermophilus #1 Cas9 expression, human optimized
pcDNA3.3 TOPO
|
| M-NMcas | Cas9 (Other) | CMV | Neomycin (select with G418) |
Church
| Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
|
Mammalian N. meningitidis Cas9 expression, human optimized
pcDNA3.3 hygro
|
| pCW-Cas9 | humanized S. pyogenes Cas9 | Tet ON | Puromycin |
Sabatini
| Genetic screens in human cells using the CRISPR-Cas9 system.
Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
|
Doxycycline-inducible lentiviral expression of SpCas9
pCW57.1
|
| pCAG-hCas9 | hCas9 | CAG | Neomycin (select with G418) |
Hatada
| Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system.
Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 10.3390/ijms141019774.
|
Expresses hCas9 under the CAG promoter for CRISPR
pEGFP-N1
|
| lentiCRISPR v2 | Cas9 (Synthetic), Puromycin resistance | EFS-NS, EFS-NS | Puromycin |
Zhang
| Improved vectors and genome-wide libraries for CRISPR screening.
Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
|
Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
Custom
|
| lentiCas9-Blast | Cas9 (Synthetic), Blasticidin resistance | EFS-NS, EFS-NS | Blasticidin |
Zhang
| Improved vectors and genome-wide libraries for CRISPR screening.
Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
|
Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
pFUGW
|
| pLenti-OC-IRES-BSD | OCT1 (Homo sapiens), Cas9 (Other) | CMV | Blasticidin |
Wei
| High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells.
Nature. 2014 Apr 9. doi: 10.1038/nature13166.
|
To create stable cell clones with high-level expression of Cas9 and OCT1
pLenti-CMV-BSD
POU2F1 OCT1, OTF1, oct-1B
|
| pLV hUbC-Cas9-T2A-GFP | humanized Cas9 T2A GFP (Other) | hUbC | |
Gersbach
| Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Nucleic Acids Res. 2014 Aug 13. pii: gku749.
|
3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP
FUGW
|
| pSQT834 | Csy4-T2A-Cas9-NLS (Homo sapiens) | CAG | |
Joung
| Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.
Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
|
Csy4 and Cas9 nuclease expression plasmid
pCAG-CFP
|
| pSQT817 | Cas9 | CAG | |
Joung
| Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.
Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
|
wildtype Cas9 expression plasmid
pCAG-CFP
|
| pL-CRISPR.EFS.GFP | SpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-eGFP (Synthetic) | EFS, hU6 | |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven
pLKO.005
|
| pL-CRISPR.EFS.tRFP | SpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-tRFP (Synthetic) | | |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven
pLKO.005
|
| pLKO5d.EFS.SpCas9.P2A.BSD | SpCas9 (Other), EFS (Homo sapiens), P2A-BSD (Synthetic) | | Blasticidin |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven
pLKO.005
|
| pL-CRISPR.SFFV.tRFP | SpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-tRFP (Synthetic) | | |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven
pLKO.005
|
| pL-CRISPR.SFFV.GFP | SpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-eGFP (Synthetic) | | |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven
pLKO.005
|
| pL-CRISPR.EFS.PAC | SpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-PAC (Synthetic) | | Puromycin |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven
pLKO.005
|
| pL-CRISPR.SFFV.PAC | SpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-PAC (Synthetic) | | Puromycin |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven
pLKO.005
|
| pAdSh.PGK.Cas9 | Human codon-optimized S. pyogenes Cas9 (Synthetic) | | Neomycin (select with G418) |
Goncalves
| Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells.
Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
|
Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter
pBR322-based pAdEasy backbone
|
| pLKO5d.EFS.SpCas9.P2A.PAC | SpCas9 (Other), EFS (Homo sapiens), P2A-PAC (Synthetic) | | Puromycin |
Ebert
| Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing.
Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
|
Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven
pLKO.005
|
| Puro-Cas9 donor | hSpCas9 (Other) | | Puromycin |
Huangfu
| An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells.
Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018.
|
Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus
N/A
|
| pX330A-1x2 | humanized S. pyogenes Cas9 nuclease (Other) | CBh | |
Yamamoto
| Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system.
Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
|
Expresses Cas9 nuclease and gRNA
pUC ori vector
|
| piCRg Entry | hSpCas9 | | |
Huangfu
| An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells.
Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018.
|
Cas9/gRNA Entry expression plasmid
Low copy
|
| pCAG-T3-hCASeGFP-pA | CAS9 (Synthetic) | CAG | |
Fujii
| Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease.
Nucleic Acids Res. 2013 Aug 30.
|
Expresses CAS9-eGFP fusion protein in mammalian cells.
pCAGGS
|
| Eef1a-1955/-1>nls::Cas9::nls | nls::Cas9::nls (Other) | Ciinte.Eef1a (EF1alpha) -1955/-1 | |
Christiaen
| Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
|
Eef1a (EF1alpha) promoter driving nls::Cas9::nls
pCESAx
|
| Mesp-1916/-1>nls::Cas9::nls | nls::Cas9::nls (Other) | Ciinte.Mesp -1916/-1 | |
Christiaen
| Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
|
Mesp driver driving nls::Cas9::nls
pCESAx
|
| Sox1/2/3-2373/+3>nls::Cas9::nls | nls::Cas9::nls (Other) | Ciinte.Sox1/2/3 (SoxB1) -2373/+3 | |
Christiaen
| Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
|
Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls
pCESAx
|
| pHL-EF1a-SphcCas9-iP-A | CRISPR Cas9 (Other) | | Puromycin |
Hotta
| Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9.
Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013.
|
Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
pHL-EF1a-GW-iP-A
|
| pSECC | Cas9 (Other), Cre | EFS, EFS (after Cas9-2A) | |
Jacks
| Rapid modelling of cooperating genetic events in cancer through somatic genome editing.
Nature. 2014 Oct 22. doi: 10.1038/nature13906.
|
3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
pLL3.3
|
| PX551 | SpCas9 (Synthetic) | pMecp2 | |
Zhang
| In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9.
Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
|
pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
pAAV
|
| LSL-Cas9-Rosa26TV | Cas9 (Synthetic), EGFP | CAG, CAG | Neomycin (select with G418) ; DTA |
Zhang
| CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling.
Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
|
Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
Ai9
|
| pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA | hSaCas9 (Other), Chimeric guide for SaCas9 (Other) | | |
Zhang
| In vivo genome editing using Staphylococcus aureus Cas9.
Nature. 2015 Apr 1. doi: 10.1038/nature14299.
|
A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
pAAV
|
| pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA | hSaCas9 (Other) | | |
Zhang
| In vivo genome editing using Staphylococcus aureus Cas9.
Nature. 2015 Apr 1. doi: 10.1038/nature14299.
|
A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).
pAAV
|
| pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA | hSaCas9 (Other), Chimeric guide for SaCas9 (Other) | | |
Zhang
| In vivo genome editing using Staphylococcus aureus Cas9.
Nature. 2015 Apr 1. doi: 10.1038/nature14299.
|
A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
pAAV
|
| pMuLE ENTR CMV-hCas9 R4-R3 | human codon optimized Cas9 (Synthetic) | CMV | |
Frew
| A versatile modular vector system for rapid combinatorial mammalian genetics.
J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
|
MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning
N/A
|
| pMuLE ENTR SV40-hCas9 L3-L2 | human codon optimized Cas9 (Synthetic) | SV40 | |
Frew
| A versatile modular vector system for rapid combinatorial mammalian genetics.
J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
|
MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
N/A
|
| pMuLE ENTR SV40-hCas9 L5-L2 | human codon optimized Cas9 (Synthetic) | SV40 | |
Frew
| A versatile modular vector system for rapid combinatorial mammalian genetics.
J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
|
MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
N/A
|
| c3GIC9 | humanized S. Pyogenes Cas9 (Other) | TRE3G | |
Dow
| Inducible in vivo genome editing with CRISPR-Cas9.
Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 2015 Feb 18.
|
col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
col1a1 Flp-in targeting construct
|
| ptreCas9-mKate2ps-T1gRNA | Cas9–mKate2ps (Synthetic) | | |
Bleris
| CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells.
Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
|
Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA
pTag-CFP
|
| pCas9–mKate2ps–EgRNA | Cas9–mKate2ps (Synthetic) | | |
Bleris
| CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells.
Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
|
Empty; no U6 gRNA cassette
pTag-CFP
|
| pCas9–mKate2ps–T1gRNA | Cas9–mKate2ps (Synthetic) | | |
Bleris
| CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells.
Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
|
t1 gRNA (ATGAGAATCAAGGCGGTCGA)
pTag-CFP
|
| PX851 | SpCas9 (aa 2-535) | CBh | |
Zhang
| A split-Cas9 architecture for inducible genome editing and transcription modulation.
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
|
N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).
PX330
|
| PX852 | SpCas9 (aa536-1368) | CBh | |
Zhang
| A split-Cas9 architecture for inducible genome editing and transcription modulation.
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
|
C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
PX330
|
| PX853 | SpCas9 (aa 2-573) | CBh | |
Zhang
| A split-Cas9 architecture for inducible genome editing and transcription modulation.
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
|
N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).
PX330
|
| PX854 | SpCas9 (aa573-1368) | CBh | |
Zhang
| A split-Cas9 architecture for inducible genome editing and transcription modulation.
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
|
C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
PX330
|
| pLSC-5 | SpCas9(574-1368), SpCas9(2-573) | EFS, EFS | Puromycin |
Zhang
| A split-Cas9 architecture for inducible genome editing and transcription modulation.
Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
|
Lenti vector for expression of inducible split Cas9
lentiCRISPR
|
| pSpCas9(BB)-2A-Puro (PX459) V2.0 | hSpCas9-2A-Puro V2.0 (Synthetic) | Cbh | Puromycin |
Zhang
| Genome engineering using the CRISPR-Cas9 system.
Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
|
Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
PX459
|
| lentiCas9-EGFP | Cas9 (Synthetic), EGFP (Other) | EFS-NS, EFS-NS | |
Zhang
| Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis.
Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5.
|
Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.
pFUGW
|
| pX330S-2-PITCh | humanized S. pyogenes Cas9 (Other) | CBh | |
Yamamoto
| MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17.
|
Expresses Cas9 nuclease and the PITCh-gRNA
pUC ori vector
|
| pX330A-FBL/PITCh | humanized S. pyogenes Cas9 (Other) | CBh | |
Yamamoto
| MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17.
|
Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA
pUC ori vector
|
| Adeno Cas9 | | U6 and CBh | |
Ventura
| In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.
Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22.
|
Adenoviral vector for the expression of the Cas9
pacAd5
Tag
/ Fusion Protein |
| pX333 | | CBh; U6 | |
Ventura
| In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.
Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22.
|
Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.
pX333
Tag
/ Fusion Protein- 3XFLAG-Cas9 (N terminal on backbone)
|
| pKMD106e - intein-Cas9(S219) | mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG (Other) | CMV | |
Liu
| Small molecule-triggered Cas9 protein with improved genome-editing specificity.
Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6.
|
Expresses intein-Cas9(S219) in mammalian cells
pJDS246
|
| pKMD106h - intein-Cas9(C574) | mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG (Other) | CMV | |
Liu
| Small molecule-triggered Cas9 protein with improved genome-editing specificity.
Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6.
|
Expresses intein-Cas9(C574) in mammalian cells
pJDS246
|
| pKMD111e - intein-Cas9(S219-G521R) | mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG (Other) | CMV | |
Liu
| Small molecule-triggered Cas9 protein with improved genome-editing specificity.
Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6.
|
Expresses intein-Cas9(S219-G521R) in mammalian cells
pJDS246
|
| pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI) | Cas9 (Other), hU6 promoter; BbsI sites for sgRNA, H1 promoter; BamHI site for shRNA | CBh, U6, H1 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
pU6-(BbsI)_CBh-Cas9-T2A-BFP
|
| pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B | Cas9 (Other), sgRNA cassette | CBh, U6 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A
pU6-(BbsI)_CBh-Cas9-T2A-BFP
|
| pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B | Cas9 (Other), sgRNA targeting ROSA26-1 | CBh, U6 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
|
| pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6 | Cas9 (Other), sgRNA cassette | CBh, U6 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A
pU6-(BbsI)_CBh-Cas9-T2A-BFP
|
| pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B | Cas9 (Other), sgRNA cassette | CBh, U6 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
|
| pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6 | Cas9 (Other), sgRNA cassette | CBh, U6 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
|
| pU6-(BbsI)_CBh-Cas9-T2A-BFP | Cas9 (Other), sgRNA cassette | CBh, U6 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide
pX330
|
| pU6-(BbsI)_CBh-Cas9-T2A-mCherry | Cas9 (Other), sgRNA cassette | CBh, U6 | |
Kuehn
| Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
|
Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide
pX330
|
| pMCS-rybozyme-IRES-CAS9 | CAS9 (Synthetic), HDV ribozyme (Other) | | |
Fujii
| Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells.
Sci Rep. 2015 Dec 16;5:18341. doi: 10.1038/srep18341.
|
Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.
pCAGGS
|
| pSaCas9_GFP | SaCas9-2A-GFP (Synthetic) | CAG | |
Musunuru
|
Staphylococcus aureus Cas9
(unpublished)
|
Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
pCAG
|
| pCas9–mKate2ps–CgRNA | Cas9–mKate2ps | | |
Bleris
| CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells.
Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
|
Control gRNA (GTCAAGGCACTCTTGCCTA)
pTag-CFP
|
| MSCV_Cas9_puro | Cas9 (Synthetic) | MSSV_LTR | Puromycin |
Vakoc
| Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains.
Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235.
|
Retroviral introduction of Cas9 into mammalian cell line.
MSCV_puro
|
| MSP469 | mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag (Other) | CMV & T7 | |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
JDS246
Tags
/ Fusion Proteins- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
|
| MSP680 | mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag (Other) | CMV & T7 | |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
JDS246
Tags
/ Fusion Proteins- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
|
| MSP1101 | mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag (Other) | CMV & T7 | |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
JDS246
Tags
/ Fusion Proteins- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
|
| MSP977 | mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag (Other) | CMV & T7 | |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
JDS246
Tags
/ Fusion Proteins- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
|
| MSP1594 | mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS (Other) | CAG | |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS
pCAG-CFP
Tag
/ Fusion Protein- NLS (C terminal on insert)
|
| BPK2139 | mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag (Other) | CAG | |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Human expression vector for S .aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG
pCAG-CFP
Tags
/ Fusion Proteins- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
|
| pBGK | Cas9 | | |
Ohtsuka
| Mouse Genome Editing Using the CRISPR/Cas System.
Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83.
|
Vector for Cas9 mRNA synthesis
pcDNA3.1
|
| pCAS9-mCherry-Frame +0 | gRNA frame +0 | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
CRISPaint frame selector +0
pCAS9-mCherry
|
| pCAS9-mCherry-Frame +1 | gRNA frame +1 | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
CRISPaint frame selector +1
pCAS9-mCherry
|
| pCAS9-mCherry-Frame +2 | gRNA frame +2 | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
CRISPaint frame selector +2
pCAS9-mCherry
|
| pCAS9-mCherry-TUBB | gRNA TUBB | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
CRISPaint target selector TUBB
pCAS9-mCherry
|
| pCAS9-mCherry-ACTG1 | gRNA ACTG1 (Homo sapiens) | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
CRISPaint target selector ACTG1
pCAS9-mCherry
|
| pCAS9-mCherry-HIST1H4C | gRNA HIST1H4C (Homo sapiens) | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
CRISPaint target selector HIST1H4C
pCAS9-mCherry
|
| pKLV2-EF1a-BsdCas9-W | EF1a-Cas9 cassette, WPRE (Homo sapiens) | EF1a | |
Yusa
| A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
|
Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus
pKLV2 lentiviral vector
|
| pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W | U6gRNA cassette, PGKGFP2ABFP cassette, WPRE (Homo sapiens) | | |
Yusa
| A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
|
Cas9 activity reporter (control) with GFP and BFP
pKLV2 lentiviral vector
|
| pRosa26-EF1a-hCas9IRESneo | EF1a-hCas9IRESneopA (Synthetic) | | |
Yusa
| A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
|
Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette
pBluescriptII
|
| phumanCas9 (GB0575) | Cas9 coding region (human codon optimised) (Other) | | |
Orzaez
| GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.
Plant Physiol. 2013 Jul;162(3):1618-31.
|
Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part
pUPD
|
| PX377 Corynebacter diphtheriae Cas9 | CdCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Corynebacter diphtheriae Cas9
pDREAM
|
| PX378 Sutterella wadsworthensis Cas9 | SwCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Sutterella wadsworthensis Cas9
pDREAM
|
| PX379 Legionella pneumophila str. Paris Cas9 | LpCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Legionella pneumophila str. Paris Cas9
pDREAM
|
| PX380 Treponema denticola Cas9 | TdCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Treponema denticola Cas9
pDREAM
|
| PX381 Filifactor alocis Cas9 | FaCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Filifactor alocis Cas9
pDREAM
|
| PX382 Staphylococcus pseudintermedius Cas9 | SpsCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Staphylococcus pseudintermedius Cas9
pDREAM
|
| PX383 Lactobacillus johnsonii Cas9 | LjCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Lactobacillus johnsonii Cas9
pDREAM
|
| PX385 Streptococcus pasteurianus Cas9 | SpaCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Streptococcus pasteurianus Cas9
pDREAM
|
| PX386 Lactobacillus farciminis Cas9 | LfCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Lactobacillus farciminis Cas9
pDREAM
|
| PX387 Mycoplasma mobile Cas9 | MmCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Mycoplasma mobile Cas9
pDREAM
|
| PX388 Bacteroides coprophilus Cas9 | BcCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Bacteroides coprophilus Cas9
pDREAM
|
| PX389 Fluviicola taffensis Cas9 | FtCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Fluviicola taffensis Cas9
pDREAM
|
| PX390 Flavobacterium columnare Cas9 | FcCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Flavobacterium columnare Cas9
pDREAM
|
| PX391 Sphaerochaeta globus str. Buddy Cas9 | SgCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Sphaerochaeta globus str. Buddy Cas9
pDREAM
|
| PX392 Azospirillum B510 Cas9 | AzoCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Azospirillum B510 Cas9
pDREAM
|
| PX393 Gluconacetobacter diazotrophicus Cas9 | GdCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Gluconacetobacter diazotrophicus Cas9
pDREAM
|
| PX394 Neisseria cinerea Cas9 | NcCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Neisseria cinerea Cas9
pDREAM
|
| PX395 Roseburia intestinalis Cas9 | RiCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Roseburia intestinalis Cas9
pDREAM
|
| PX396 Parvibaculum lavamentivorans Cas9 | PlCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Parvibaculum lavamentivorans Cas9
pDREAM
|
| PX398 Nitratifractor salsuginis str DSM 16511 Cas9 | NsCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Nitratifractor salsuginis str DSM 16511 Cas9
pDREAM
|
| PX399 Mycoplasma gallisepticum str. F Cas9 | MgCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Mycoplasma gallisepticum str. F Cas9
pDREAM
|
| PX400 Campylobacter lari CF89-12 Cas9 | ClCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Campylobacter lari CF89-12 Cas9
pDREAM
|
| PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9 | St3Cas9 (Synthetic) | CBh | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Streptococcus thermophilus LMD-9 CRISPR 3 Cas9
Unknown
|
| PX404 Campylobacter jejuni Cas9 | CjCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Campylobacter jejuni Cas9
Unknown
|
| pKLV2-EF1a-Cas9Bsd-W | EF1a-Cas9 cassette, WPRE (Homo sapiens) | EF1a | |
Yusa
| A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
|
Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus
pKLV2 lentiviral vector
|
| LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR | puromycin (Synthetic), hCas9 (Synthetic) | CAG, CAG | Puromycin |
Elverløv-Jakobsen
|
Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
(unpublished)
|
A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
Amp
|
| px330_SMAD exon 9 gRNA | SMAD exon 9 gRNA (Synthetic) | U6 | |
Elverløv-Jakobsen
|
Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
(unpublished)
|
px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
amp
|
| CAS9PBKS | Cas9 (Other) | CbAkt | |
Bennett
| Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis.
Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16.
|
CbAkt promoter driven Cas9-2A-GFP (from px458)
pBKS
|
| Cas9-2A-Cre | Cas9 and Cre recombinase (Homo sapiens) | CAG | |
Zhang
| Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9.
Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.
|
co-expressing Cas9 and Cre recombinase
Cas9-2A-GFP
|
| PX405 Neisseria meningitidis Cas9 | NmCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Neisseria meningitidis Cas9
pDREAM
|
| PX406 Pasteurella multocida Cas9 | PmCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Pasteurella multocida Cas9
pDREAM
|
| PX408 Francisella tularensis subsp. novicida Cas9 | FtCas9 (Synthetic) | CMV | |
Zhang
|
Feng Zhang lab bacterial Cas9 orthologs
(unpublished)
|
Francisella tularensis subsp. novicida Cas9
pDREAM
|
| pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268 | Zif268 (Mus musculus) | | |
Wolfe
| DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
|
Expresses wild type SpCas9 fused to Zif268 in mammalian cells
pCSDest2-SpCas9-NLS-3XHA-NLS
Egr1 A530045N19Rik, ETR10, ETR103, Egr-, Egr-1, Krox, Krox-, Krox-1, Krox-24, Krox24, NG, NGF, NGF1, NGF1-A, NGFI-A, NGFIA, TI, TIS8, Zen, Zenk, Zfp-, Zfp-6, Zif26, Zif268, egr
|
| pCSDest2-SpCas9-NLS-3XHA-NLS | SpCas9 (Other) | | |
Wolfe
| DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
|
Expresses wild type SpCas9 in mammalian cells
pCSDest2
|
| pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2 | TS2 ZFP (Synthetic) | | |
Wolfe
| DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
|
Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells
pCSDest2-SpCas9-NLS-3XHA-NLS
|
| pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3 | TS3 ZFP (Synthetic) | | |
Wolfe
| DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
|
Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells
pCSDest2-SpCas9-NLS-3XHA-NLS
|
| pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4 | TS4 ZFP (Synthetic) | | |
Wolfe
| DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
|
Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells
pCSDest2-SpCas9-NLS-3XHA-NLS
|
| pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3 | TS3 ZFP (Synthetic) | | |
Wolfe
| DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
|
Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
|
| pY004 (pcDNA3.1-hFnCpf1) | hFnCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized FnCpf1
pcDNA3.1
|
| pY005 (pcDNA3.1-hLb3Cpf1) | hLb3Cpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized Lb3Cpf1
pcDNA3.1
|
| pY006 (pcDNA3.1-hBpCpf1) | hBpCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized BpCpf1
pcDNA3.1
|
| pY007 (pcDNA3.1-hPeCpf1) | hPeCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized PeCpf1
pcDNA3.1
|
| pY008 (pcDNA3.1-hPbCpf1) | hPbCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized LPbCpf1
pcDNA3.1
|
| pY009 (pcDNA3.1-hSsCpf1) | hSsCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized SsCpf1
pcDNA3.1
|
| pY010 (pcDNA3.1-hAsCpf1) | hAsCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized AsCpf1
pcDNA3.1
|
| pY011 (pcDNA3.1-hLb2Cpf1) | hLb2Cpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized Lb2Cpf1
pcDNA3.1
|
| pY012 (pcDNA3.1-hCMtCpf1) | hCMtCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized CMtCpf1
pcDNA3.1
|
| pY013 (pcDNA3.1-hEeCpf1) | hEeCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized EeCpf1
pcDNA3.1
|
| pY014 (pcDNA3.1-hMbCpf1) | hMbCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized MbCpf1
pcDNA3.1
|
| pY015 (pcDNA3.1-hLiCpf1) | hLiCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized LiCpf1
pcDNA3.1
|
| pY016 (pcDNA3.1-hLbCpf1) | hLbCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized LbCpf1
pcDNA3.1
|
| pY017 (pcDNA3.1-hPcCpf1) | hPcCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized PcCpf1
pcDNA3.1
|
| pY018 (pcDNA3.1-hPdCpf1) | hPdCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized PdCpf1
pcDNA3.1
|
| pY019 (pcDNA3.1-hPmCpf1) | hPmCpf1 (Other) | CMV | Neomycin (select with G418) |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Expresses humanized PmCpf1
pcDNA3.1
|
| FUCas9Cherry | humanised S.pyogenes cas9 (Other) | hUbC | |
Herold
| An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo.
Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26.
|
Expresses Cas9 with fluorescent Cherry reporter
FUGW
|
| lentiCas9-Venus | Cas9 (Synthetic), Venus Reporter (Synthetic) | EFS-NS, P2A | Venus |
Bauer
| BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis.
Nature. 2015 Sep 16. doi: 10.1038/nature15521.
|
Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.
pFUGW
|
| MSP1830 | mammalian codon-optimized KKH variant Staphylococcus aureus Cas9(E782K/N968K/R1015H)-NLS-3xFlag (Other) | CAG | |
Joung
| Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition.
Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
|
Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
pCAG-CFP
|
| spCas9-BlastR (pCBhCas9-BlastR) | spCas9 | CBh | Blasticidin |
Sherwood
| Cloning-free CRISPR.
Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 10.1016/j.stemcr.2015.09.022.
|
Expresses SpCas9 and Blasticidin resistance
p2Tol2
|
| pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110) | humanized S. pyogenes Cas9 fused to human Geminin (Homo sapiens) | CBh | |
Draetta
| Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair.
Cell Rep. 2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 10.1016/j.celrep.2016.01.019.
|
A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid
pX330-U6-Chimeric_BB-CBh-hSpCas9
GMNN Gem, MGORS6
|
| eSpCas9(1.1) | enhanced specificity Cas9 (1.1) (Other) | CBh | |
Zhang
| Rationally engineered Cas9 nucleases with improved specificity.
Science. 2015 Dec 1. pii: aad5227.
|
Expresses high specificity SpCas9. Px330-like plasmid.
pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pCR1002 | T7pr_10xHis-MBP-TEV-SPyCas9 | | |
Corn
| Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
|
Express Streptococcus pyogenes Cas9
SPynCas9
|
| VP12 | mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag (Other) | CMV | |
Joung
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Nature. 2016 Jan 6. doi: 10.1038/nature16526.
|
Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
JDS246
|
| MSP2135 | mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag (Other) | CMV | |
Joung
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Nature. 2016 Jan 6. doi: 10.1038/nature16526.
|
Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
JDS246
|
| MSP2133 | mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag (Other) | CMV | |
Joung
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Nature. 2016 Jan 6. doi: 10.1038/nature16526.
|
Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
JDS246
|
| MSP2440 | mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag (Other) | CMV | |
Joung
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Nature. 2016 Jan 6. doi: 10.1038/nature16526.
|
Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG
JDS246
|
| BPK2797 | mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag (Other) | CMV | |
Joung
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Nature. 2016 Jan 6. doi: 10.1038/nature16526.
|
Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
JDS246
|
| MSP2443 | mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag (Other) | CMV | |
Joung
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Nature. 2016 Jan 6. doi: 10.1038/nature16526.
|
Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
JDS246
|
| pCas9-polyA | Cas9-polyadenine (Other) | T7 | |
Mashimo
| ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.
Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.
|
express hCas9 byT7 promoter with polyadenine tails
pcDNA3.3-TOPO
|
| MSP2372 | mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag (Other) | CMV | |
Joung
| High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Nature. 2016 Jan 6. doi: 10.1038/nature16526.
|
Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
JDS246
|
| Lenti‐Cas9‐2A‐Blast | Cas9 (Synthetic) | EFS-NS | Blasticidin |
Moffat
| High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.
Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
|
Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter
lenti CRISPR pXPR_001
|
| pAAVS1-PDi-CRISPRn | Cas9 (Synthetic), rtTA (Synthetic) | TRE, CAG | Puromycin |
Conklin
| CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
|
Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus
pAAVS1
|
| pAWp30 | EFSp-Cas9-P2A-Zeo | | |
Lu
| Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM.
Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883.
|
pFUGW-EFSp-Cas9-P2A-Zeo
pFUGW
|
| pTE4396 | As crRNA, AsCpf1 | human U6, CMV | Neomycin (select with G418) |
Welker
| Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
|
Expresses human codon-optimized AsCpf1 and As crRNA.
pcDNA3.1
|
| pTE4398 | Lb crRNA, LbCpf1 | human U6, CMV | Neomycin (select with G418) |
Welker
| Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
|
Expresses human codon-optimized LbCpf1 and Lb crRNA.
pcDNA3.1
|
| pBLO1811_Cas9_noNLS_human | Cas9 (Homo sapiens) | | |
Savage
| Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
|
Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA
px330
|
| pBLO1806_Cas9_2xNLS_human | Cas9 (Homo sapiens) | | |
Savage
| Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
|
Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA
px330
|
| pLentiCRISPR-EGFP | | | |
Bornhauser
| Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL.
Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
|
Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance
lentiCRISPRv1
Tag
/ Fusion Protein |
| pLentiCRISPR-tagBFP | | | |
Bornhauser
| Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL.
Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
|
Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance
lentiCRISPRv1
Tag
/ Fusion Protein |
| pLentiCRISPR-mCherry | | | |
Bornhauser
| Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL.
Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
|
Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.
lentiCRISPRv1
Tag
/ Fusion Protein |
| pLentiCRISPR-RFP657 | | | |
Bornhauser
| Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL.
Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
|
Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance
lentiCRISPRv1
Tag
/ Fusion Protein |
| FUG-T2A-Cas9 | Cas9 | Ubiquitin | |
Luikart
| A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons.
Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
|
Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
FUGW
|
| pRubiC-T2A-Cas9 | Cas9 | Ubiquitin | |
Luikart
| A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons.
Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
|
Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
pRubi
|
| pRubiG-T2A-Cas9 | Cas9 | Ubiquitin | |
Luikart
| A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons.
Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
|
Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
pRubi
|
| PXL | Cas9 | U6 | |
Luikart
| A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons.
Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611.
|
PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
PX330
|
| pX330-BsaIx2-DD-Cas9 | DD-SpCas9 (Other) | CAG | |
Calos
| In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining.
Nucleic Acids Res. 2016 Jan 13. pii: gkv1542.
|
Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1
modified pX330
|
| pCas9-VRER_2A_GFP | Cas9-VRER (Synthetic) | CAG | |
Tessier-Lavigne
| Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9.
Nature. 2016 May 5;533(7601):125-9. doi: 10.1038/nature17664. Epub 2016 Apr 27.
|
Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control
pCAG
|
| pXPR_BRD111 | Cas9 | | Blasticidin |
Hahn
| Integrated genetic and pharmacologic interrogation of rare cancers.
NATURE COMMUNICATIONS 7:11987
|
Expresses Cas9 with the pLX311 backbone
pLX311
|
| CAG-Cas9-T2A-EGFP-ires-puro | WT SpCas9-T2A-EGFP-ires-puromycin resistance (Other) | CAG | Puromycin |
Otonkoski
| An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation.
Cell Rep. 2017 Apr 11;19(2):281-294. doi: 10.1016/j.celrep.2017.03.055.
|
Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.
PyCAG
|
| pLentiCas9-T2A-BFP | T2A-BFP | In frame with Cas9 | Blasticidin |
Guigo
| Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
|
Cas9 protein with BFP
Lenti-Cas9-blast
|
| pLentiCas9-T2A-GFP | T2A-GFP | In frame with Cas9 | Blasticidin |
Guigo
| Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
|
Cas9 protein with GFP
Lenti-Cas9-blast
|
| pZac2.1 SV40-CMV-SaCas9-3xNLS | SV40-CMV-SaCas9-3xNLS (Synthetic) | SV40, CMV promoters | |
Wagers
| In vivo gene editing in dystrophic mouse muscle and muscle stem cells.
Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177. Epub 2015 Dec 31.
|
AAV vector containing SaCas9
pZac2.1
|
| SQT1659 | mammalian codon-optimized Acidaminococcus sp. BV3L6 Cpf1-NLS-3xHA (Other) | CAG | |
Joung
| Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells.
Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
|
Human expression plasmid for AsCpf1-NLS-3xHA
pCAG-GFP
|
| SQT1665 | mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA (Other) | CAG | |
Joung
| Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells.
Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
|
Human expression plasmid for LbCpf1-NLS-3xHA
pCAG-GFP
|
| pLentiCRISPR-E | | | Puromycin |
Abbosh
| Differential effects of clinically-relevant N- vs C-terminal truncating CDKN1A mutations on cisplatin sensitivity in bladder cancer.
Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 10.1158/1541-7786.MCR-19-1200.
|
Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette
pLentiCRISPRV2
Tag
/ Fusion Protein |
| pCAG-SpCas9-GFP-U6-gRNA | SpCas9 | CAG | |
Zou
| The ribosomal prolyl-hydroxylase OGFOD1 decreases during cardiac differentiation and modulates translation and splicing.
JCI Insight. 2019 May 21;5. pii: 128496. doi: 10.1172/jci.insight.128496.
|
All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC
pSpCas9(BB)-2A-GFP (PX458)
|
| pCAG-eCas9-GFP-U6-gRNA | eSpCas9(1.1) | CAG | |
Zou
|
pCAG-SpCas9-U6-gRNA
(unpublished)
|
All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
eSpCas9(1.1)
|
| eSpCas9(1.1)_No_FLAG | | CBh | |
Doyon
| A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.
Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009.
|
FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.
pX330-U6-Chimeric_BB-CBh-hSpCas9
Tag
/ Fusion Protein |
| pTE4495 | Mb crRNA (Other), MbCpf1 | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
mammalian expression MbCpf1 nuclease and Mb crRNA
pcDNA3.1
|
| pTE4497 | Fn crRNA (Other), FnCpf1 | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
mammalian expression FnCpf1 nuclease and Fn crRNA
pcDNA3.1
|
| pDNR-SpCas9-Gem | SpCas9-Gem (Other) | T7 | |
Little
| A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells.
Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
|
Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
pDNR-Dual
|
| pDNR-SpCas9 | SpCas9 (Other) | T7 | |
Little
| A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells.
Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
|
Used for in vitro transcription of SpCas9 mRNA.
pDNR-Dual
|
| pDNR-SpCas9-Cdt1 | SpCas9-Cdt1 (Other) | | |
Little
| A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells.
Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4.
|
Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.
pDNR-Dual
|
| peSpCas9(1.1)-2×sgRNA (empty, donor) | | | |
Nakayama
| Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.
Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051.
|
All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.
eSpCas9(1.1)
|
| pAAV-SMVP-Cas9N | Cas9N (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Expresses Cas9N in mammalian cells.
pZac2.1
|
| pAAV-SMVP-Cas9C | Cas9C (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Expresses Cas9C in mammalian cells.
pZac2.1
|
| pAAV-CASI-Cas9C | Cas9C (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.
pZac2.1
|
| pSMVP-Cas9N | Cas9N (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Plasmid that expresses Cas9N in mammalian cells.
pMAX
|
| pSMVP-Cas9C-P2A-turboGFP | Cas9C (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.
pMAX
|
| pSMVP-Cas9C | Cas9C (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Plasmid that expresses Cas9C in mammalian cells.
pMAX
|
| pSMVP-Cas9FL-P2A-turboGFP | Cas9FL (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.
pMAX
|
| pAAV-CASI-Cas9C-P2A-turboGFP | Cas9C (Synthetic) | | |
Church
| A multifunctional AAV-CRISPR-Cas9 and its host response.
Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
|
Expresses Cas9C in mammalian cells; co-expresses turboGFP.
pZac2.1
|
| pRZ-CAS9-mCherry | Cas9 (Synthetic) | CMV | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
Cas9, mCherry expression
pRZ
|
| pCAS9-mCherry empty | | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
Cas9, mCherry, and sgRNA expression
pCAS9
|
| pCAS9-BFP empty | | | |
Hornung
| CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.
Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
|
Cas9, TagBFP, and sgRNA expression
pCAS9
|
| Construct 7 - CMVp-Cas9-3xNLS-HSVpA | Cas9-3xNLS (Homo sapiens) | | |
Lu
| Continuous genetic recording with self-targeting CRISPR-Cas in human cells.
Science. 2016 Aug 18. pii: aag0511.
|
Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression
pGL2-Luc (Addgene #26280)
|
| Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR | Cas9-3xNLS (Homo sapiens) | | Puromycin |
Lu
| Continuous genetic recording with self-targeting CRISPR-Cas in human cells.
Science. 2016 Aug 18. pii: aag0511.
|
Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection
Lentiviral
|
| Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR | Cas9-3xNLS | | Puromycin |
Lu
| Continuous genetic recording with self-targeting CRISPR-Cas in human cells.
Science. 2016 Aug 18. pii: aag0511.
|
Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection
Lentiviral
|
| LentiCRISPRv2Cre | Cre Recombinase (Other) | EFS (P2A) | |
Feldser
| Systematic in vivo inactivation of chromatin regulating enzymes identifies Setd2 as a potent tumor suppressor in lung adenocarcinoma.
Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 10.1158/0008-5472.CAN-16-2159.
|
Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site
lentiCRISPR v2 (Addgene #52961)
|
| LentiCRISPRv2GFP | GFP (Other) | EFS (P2A) | |
Feldser
| Systematic in vivo inactivation of chromatin regulating enzymes identifies Setd2 as a potent tumor suppressor in lung adenocarcinoma.
Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 10.1158/0008-5472.CAN-16-2159.
|
3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site
lentiCRISPR v2
|
| lentiCRISPR v2-Blast | Blasticidin S deaminase | EF-1α | Blasticidin |
Babu
|
CRISPR/Cas9-based system for loss-of-function screening
(unpublished)
|
Mammalian expression of Cas9 and sgRNA scaffold
lentiCRISPR v2 (#52961)
|
| pCW-Cas9-Blast | SpCas9, Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator | Tight Tre, human phosphoglycerate kinase 1 promoter | Blasticidin |
Babu
|
CRISPR/Cas9-based system for loss-of-function screening
(unpublished)
|
Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTA
pCW-Cas9 (#50661)
|
| pLV-U6-gRNA-UbC-DsRed-P2A-Bsr | DsRed-Express2, Bsr | hUbC | Blasticidin |
Gersbach
| CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
|
Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR
FUGW
|
| pLV-U6-gRNA-UbC-eGFP-P2A-Bsr | eGFP, Bsr | hUbC | Blasticidin |
Gersbach
| CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
|
Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR
FUGW
|
| pPB-US-ECasE | ERT2-spCas9-ERT2 (Other) | U6 (gRNA), EF1a (ERT2-Cas9-ERT2) | Puromycin |
Chen
|
Chen lab CRISPR plasmids
(unpublished)
|
Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
Piggybac Transposon
|
| pCRISPR-S12 | hSpCas9 (Other), eGFP (Other) | CMV, CMV (downstream of F2A self-cleaving peptide) | Puromycin, Hygromycin |
Wu
| An episomal CRISPR/Cas9 system to derive vector-free gene modified mammalian cells.
Protein Cell. 2016 Sep;7(9):689-91. doi: 10.1007/s13238-016-0299-9.
|
To episomally express codon optimized Cas9 and chimeric guide RNA
pCEP4
|
| pX601-mCherry | mCherry | T2A | |
Kan
| Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia.
Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6.
|
Staphylococcus aureus (SaCas9) conjugated with mCherry
pX601
|
| pX601-GFP | GFP (Other) | | |
Kan
| Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia.
Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6.
|
Staphylococcus aureus (SaCas9) conjugated with GFP
pX601
|
| iCas | Cas9 (Other) | CMV | OFP expression |
Tan
| A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing.
Nat Chem Biol. 2016 Sep 12. doi: 10.1038/nchembio.2179.
|
Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)
GeneArt CRISPR Nuclease vector with OFP reporter
|
| pY108 (lenti-AsCpf1) | huAsCpf1 (Synthetic), puromycin resistance gene (Synthetic) | EFS | Puromycin |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Lenti virus delivery of AsCpf1 and crRNA guide
pHKO_23
|
| pY109 (lenti-LbCpf1) | huLbCpf1 (Synthetic), puromycin resistance gene (Synthetic) | EFS | Puromycin |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Lenti virus delivery of LbCpf1 and crRNA guide
pHKO_23
|
| pY026 | huAsCpf1 (Synthetic) | CMV | |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Expresses huAsCpf1 and crRNA guide
pcDNA3.1
|
| pY027 | huLbCpf1 (Synthetic) | CMV | |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Expresses huLbCpf1 and crRNA guide
pcDNA3.1
|
| pY094 | huAsCpf1 (Synthetic), GFP (Synthetic) | CMV | |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Expresses huAsCpf1-T2A-GFP and crRNA guide
pcDNA3.1
|
| pY095 | huLbCpf1 (Synthetic), GFP (Synthetic) | CMV | |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Expresses huLbCpf1-T2A-GFP and crRNA guide
pcDNA3.1
|
| pY30 | huAsCpf1 (Synthetic), puromycin resistance gene (Synthetic) | CMV | Puromycin |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Expresses huAsCpf1-P2A-puro and crRNA guide
pcDNA3.1
|
| pY31 | huLbCpf1 (Synthetic), puromycin resistance gene (Synthetic) | CMV | Puromycin |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Expresses huLbCpf1-P2A-puro and crRNA guide
pcDNA3.1
|
| Lenti-AsCpf1-Blast | AsCpf1 (Other) | EFS-NS | Blasticidin |
Kim
| In vivo high-throughput profiling of CRISPR-Cpf1 activity.
Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
|
Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
lentiCas9-Blast(Addgene#:52962)
|
| Lenti-LbCpf1-Blast | LbCpf1 (Other) | EFS-NS | Blasticidin |
Kim
| In vivo high-throughput profiling of CRISPR-Cpf1 activity.
Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
|
Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
lentiCas9-Blast(Addgene#:52962)
|
| pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova) | loxP-stop-loxP (Other) | | |
Iwasato
| Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.
Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747.
|
This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.
pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene Plasmid #42230)
|
| Lenti-iCas9-neo | Flag-iCas9-P2A-GFP (Other) | | Neomycin (select with G418) |
Yan
| An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting.
Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25.
|
Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
pInducer-20
|
| Lenti-multi-CRISPR | | | Puromycin |
Yan
| An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting.
Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25.
|
Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression
Lenti-CRISPR
|
| pAAV-RSV-SpCas9 | pRSV (Other) | pRSV | |
Lei
| The CRISPR/Cas9-created MDM2 T309G enhances vitreous-induced expression of MDM2 and proliferation and survival of cells.
J Biol Chem. 2016 May 31. pii: jbc.M116.729467.
|
Express SpCas9 in mammalian cells
pAAV
|
| pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE | U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro (Synthetic) | | Puromycin |
Regev
| A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells.
Cell. 2016 Sep 8;166(6):1500-1511.e9. doi: 10.1016/j.cell.2016.08.052.
|
Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
pLKO
|
| FUCas9Cyan | humanised S.pyrogenes Cas9 (Other) | hUBC | |
Herold
|
(unpublished)
|
Expresses Cas9 with fluorescent Cyan reporter
FUGW
|
| FUCas9GFP | humanised S.pyogenes Cas9 (Other) | hUBC | |
Herold
|
(unpublished)
|
Expresses Cas9 with green fluorescent reporter
FUGW
|
| pLenti-Cas9-GFP | Cas9-GFP (Synthetic) | EFS | |
Sabatini
| Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras.
Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013.
|
Lentiviral vector expressing Cas9-GFP
pLentiCRISPR v1
|
| pX459-sgPLXNB2 | Plexin-B2 (Homo sapiens) | | Puromycin |
Friedel
| Plexin-B2 facilitates glioblastoma infiltration by modulating cell biomechanics.
Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4.
|
Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3
pX459
PLXNB2 MM1, Nbla00445, PLEXB2, dJ402G11.3
|
| pY036_ATP1A1_G3_Array | ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA (Other) | CBh | |
Doyon
| Marker-free coselection for CRISPR-driven genome editing in human cells.
Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265.
|
Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
pY036-U6-crRNA(BbsI)-CBh-AsCpf1
|
| pY036_ATP1A1_G5_Array | ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA (Other) | CBh | |
Doyon
| Marker-free coselection for CRISPR-driven genome editing in human cells.
Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265.
|
Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
pY036-U6-crRNA(BbsI)-CBh-AsCpf1
|
| pAAV-Neo_CAG-Cas9 | Cas9 (Other) | pCag | Neomycin (select with G418) |
Vallier
| Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.
Development. 2016 Dec 1;143(23):4405-4418.
|
Cas9 expression vector for targeting to the AAVS1 locus
AAVS1-SA-2A-NEO-CAG-RTTA3 Addgene 60431
|
| Hygro-Cas9 donor | hSpCas9 (Other) | | Hygromycin |
Huangfu
| Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development.
Cell Stem Cell. 2017 Feb 8. pii: S1934-5909(17)30001-2. doi: 10.1016/j.stem.2017.01.001.
|
Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
N/A
|
| pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA | Cas9-venus (Synthetic) | CAG | |
Kuehn
| Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9.
Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10.
|
For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein
Bluescript
|
| pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP | Cas9 (Other) | CAG | |
Kuehn
| Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9.
Methods Mol Biol. 2019;1961:137-151. doi: 10.1007/978-1-4939-9170-9_10.
|
For cloning and expression of sgRNA together with expression of Cas9 and TagRFP
Bluescript
|
| pCAG-1BPNLS-Cas9-1BPNLS | 1BPNLS-Cas9-1BPNLS (Synthetic) | CAG | |
Belmonte
| In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.
Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16.
|
BPNLS-Cas9-BPNLS expression plasmid
pCAG
|
| pCAG-1BPNLS-Cas9-1BPNLS-2AGFP | 1BPNLS-Cas9-1BPNLS-2A-EGFP (Synthetic) | CAG | |
Belmonte
| In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.
Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16.
|
BPNLS-Cas9-BPNLS-2AGFP expression plasmid
pCAG
|
| pAAV-nEFCas9 | nEF-Cas9 (Synthetic) | | |
Belmonte
| In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.
Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16.
|
SpCas9 expression AAV backbone plasmid under control of nEF promoter
PX551
|
| lenti-Cas9-VQR-Blast | SpCas9-VQR(D1135V,R1335Q,T1337R) (Other) | | Blasticidin |
Bauer
| Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci.
Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793.
|
lentiviral expression of SpCas9-VQR (NGA PAM restricted)
lentiCas9-Blast
|
| P628_SpCas9-RecA | RecA (Synthetic) | CBh | |
Luo
| Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.
J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024.
|
Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency
PX459
|
| P823_eSpCas9(1.1)-RecA | RecA | CBh | |
Luo
| Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.
J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024.
|
Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency
eSpCas9(1.1)
|
| TLCV2 | Cas9-2A-eGFP | Tight TRE promoter, Tight TRE promoter | Puromycin |
Karpf
| Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer.
Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251.
|
LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
lentiCRISPR v2
Tag
/ Fusion Protein |
| pCSDest2-NmeCas9-NLS-3XHA-NLS | Nme Cas9 (Other) | CMV | |
Sontheimer
| Naturally Occurring Off-Switches for CRISPR-Cas9.
Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8.
|
Mammalian expression of wild type Nme Cas9
pCSDest2
|
| SIN-CMV-Ca9-WPRE | spCas9 (Other) | CMV | |
Déglon
| The self-inactivating KamiCas9 system for the editing of CNS disease genes
Cell Reports , Volume 20 , Issue 12 , 2980 - 2991
|
Transfer plasmid for the production of lentiviral vectors
SIN lentiviral transfer vector
|
| SIN-PGK-Cas9-V5-WPRE | spCas9 (Other) | mouse PGK | |
Déglon
| The self-inactivating KamiCas9 system for the editing of CNS disease genes
Cell Reports , Volume 20 , Issue 12 , 2980 - 2991
|
To produce lentiviral vector for Cas9 editing
SIN lentiviral transfer vector
|
| SIN-PGK-Cas9-WPRE | hCas9 (Other) | mouse PGK | |
Déglon
| The self-inactivating KamiCas9 system for the editing of CNS disease genes
Cell Reports , Volume 20 , Issue 12 , 2980 - 2991
|
To produce Lentiviral vector for gene editing
HIV-1 lentiviral SIN transfer vector
|
| SIN-CMV-Cas9-V5-WPRE | Cas9-V5 (Other) | CMV | |
Déglon
| The self-inactivating KamiCas9 system for the editing of CNS disease genes
Cell Reports , Volume 20 , Issue 12 , 2980 - 2991
|
To produce lentiviral vector for gene editing
HIV-1 SIN lentiviral transfer vector
|
| pTE4254 | St chimeric gRNA, hStCas9 | human U6, CMV | Neomycin (select with G418) |
Welker
| Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
|
Expresses St chimeric gRNA and human codon-optimized StCas9
pcDNA3.3-TOPO
|
| pKS7107 | Nm crRNA, Nm tracrRNA, hNmCas9 | human U6, human U6, EF1a | |
Welker
| Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells.
Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
|
Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
pSimpleII
|
| pcDNA3.1-hAsCpf1(TYCV) (pY210) | Acidaminococcus sp. Cpf1 (RR variant) (Synthetic) | CMV | |
Zhang
| Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
|
Expresses humanized AsCpf1 variant that recognizes TYCV PAMs
pcDNA3.1
|
| pAsCpf1(TYCV)(BB) (pY211) | Acidaminococcus sp. Cpf1 (RR variant) (Synthetic) | CBh | |
Zhang
| Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
|
Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide
pUC ori vector
|
| pcDNA3.1-hAsCpf1(TATV) (pY220) | Acidaminococcus sp. Cpf1 (RVR variant) (Synthetic) | CMV | |
Zhang
| Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
|
Expresses humanized AsCpf1 variant that recognizes TATV PAMs
pcDNA3.1
|
| pAsCpf1(TATV)(BB) (pY221) | Acidaminococcus sp. Cpf1 (RVR variant) (Synthetic) | CBh | |
Zhang
| Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
|
Expresses humanized AsCpf1 TATV PAM variant and crRNA guide
pUC ori vector
|
| pcDNA3.1-hLbCpf1(TYCV) (pY230) | Lachnospiraceae bacterium Cpf1 (RR variant) (Synthetic) | CMV | |
Zhang
| Engineered Cpf1 variants with altered PAM specificities.
Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900.
|
Expresses humanized LbCpf1 variant that recognizes TYCV PAMs
pcDNA3.1
|
| L40C-CRISPR.EFS.dTomato | | | |
Heckl
| CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo.
Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046.
|
Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven
SIN40C
|
| L40C-CRISPR.EFS.PAC | | | Puromycin |
Heckl
| CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo.
Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046.
|
Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven
SIN40C
|
| pRGEN-CMV-CjCas9 | Cas9 derived from Campylobacter jejuni (Other) | | |
Kim
| In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni.
Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500.
|
Expression of CjCas9 in mammalian cells
pcDNA3.1
|
| CAG-Cas9 | hCas9 (Other) | CAG | |
Otonkoski
| Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9.
Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11.
|
Expresses high levels of WT Cas9 for genome editing
pCAG
|
| CAG-SaCas9-WPRE | SaCas9 (Other) | CAG | |
Otonkoski
| Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9.
Stem Cell Res. 2017 Jul;22:16-19. doi: 10.1016/j.scr.2017.05.005. Epub 2017 May 17.
|
Expresses high levels of WT SaCas9 for genome editing
pCAG
NEWENTRY
|
| DD-Cas9 with filler sequence and Venus (EDCPV) | Cas9 (Synthetic) | EFS | |
Sordella
| Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization.
Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370.
|
This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA
lentiCRISPR v2
|
| DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE) | Cas9 (Synthetic) | EFS | |
Sordella
| Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization.
Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370.
|
This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
lentiCRISPR v2
|
| pSpCas9(BB)-2A-miRFP670 | hSpCas9-2A-miRFP670 (Synthetic), BB-guide RNA (Synthetic) | Cbh, U6 | |
Kuehn
|
pSpCas9(BB)-2A-miRFP670
(unpublished)
|
Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
PX459
|
| LcV2-Hygro | | | Hygromycin |
Mendell
| An Argonaute phosphorylation cycle promotes microRNA-mediated silencing.
Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23.
|
LentiCRISPR v2 with Hygro cloned in place of Puro
LentiCRISPR v2
|
| pX330-Flag-SpCas9-HF1 (without sgRNA) | 3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| HeFm1SpCas9 | 3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| HeFm2SpCas9 | “Highly enhanced Fidelity” mut2 SpCas9 (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pY111 (pcDNA3.1-huTsCpf1) | huTsCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized TsCpf1
pcDNA3.1
|
| pY112 (pcDNA3.1-huSaCpf1) | huSaCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized SaCpf1
pcDNA3.1
|
| pY113 (pcDNA3.1-huPb2Cpf1) | huPb2Cpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized Pb2Cpf1
pcDNA3.1
|
| pY114 (pcDNA3.1-huPgCpf1) | huPgCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized PgCpf1
pcDNA3.1
|
| pY115 (pcDNA3.1-huMlCpf1) | huMlCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized MlCpf1
pcDNA3.1
|
| pY116 (pcDNA3.1-huMb2Cpf1) | huMb2Cpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized Mb2Cpf1
pcDNA3.1
|
| pY117 (pcDNA3.1-huMb3Cpf1) | huMb3Cpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized Mb3Cpf1
pcDNA3.1
|
| pY118 (pcDNA3.1-huLb4Cpf1) | huLb4Cpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized Lb4Cpf1
pcDNA3.1
|
| pY119 (pcDNA3.1-huLb5Cpf1) | huLb5Cpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized Lb5Cpf1
pcDNA3.1
|
| pY120 (pcDNA3.1-huFbCpf1) | huFbCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized FbCpf1
pcDNA3.1
|
| pY122 (pcDNA3.1-huCPbCpf1) | huCPbCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized CPbCpf1
pcDNA3.1
|
| pY123 (pcDNA3.1-huCMaCpf1) | huCMaCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized CMaCpf1
pcDNA3.1
|
| pY124 (pcDNA3.1-huBsCpf1) | huBsCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized BsCpf1
pcDNA3.1
|
| pY125 (pcDNA3.1-huBfCpf1) | huBfCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized BfCpf1
pcDNA3.1
|
| pY126 (pcDNA3.1-huBoCpf1) | huBoCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized BoCpf1
pcDNA3.1
|
| pY127 (pcDNA3.1-U6-huTsCpf1) | huTsCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized TsCpf1 and crRNA
pcDNA3.1
|
| pY128 (pcDNA3.1-U6-huMb2Cpf1) | huMb2Cpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized Mb2Cpf1 and crRNA
pcDNA3.1
|
| pY129 (pcDNA3.1-U6-huMb3Cpf1) | huMb3Cpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized Mb3Cpf1 and crRNA
pcDNA3.1
|
| pY130 (pcDNA3.1-U6-huBsCpf1) | huBsCpf1 (Other) | | |
Zhang
|
Zhang lab Cpf1 plasmids
(unpublished)
|
Expression of humanized BsCpf1 and crRNA
pcDNA3.1
|
| pX330-Flag-wtSpCas9 (without sgRNA) | 3xFlag-NLS-wild-type SpCas9-NLS (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-eSpCas9 (without sgRNA) | 3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| HeFSpCas9 | 3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS (Other) | Cbh | |
Welker
| Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
|
Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
pX330-like (without U6-sgRNA coding sequence)
|
| pCAG Cas9-2A-Citrine | Cas9 2A Citrine (Other) | | |
Sauka-Spengler
| Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo.
Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333.
|
CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.
pCAG
|
| pTK Cas9-2A-Citrine | Cas9 2A Citrine (Other) | thymidine kinase promoter | |
Sauka-Spengler
| Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo.
Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333.
|
Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
pTK
|
| pCI Cas9 H2B-RFP | Cas9 (Other) | CAG | |
Sauka-Spengler
| Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo.
Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333.
|
CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES
pCI IRES H2B RFP
|
| pXPR_206 | | | Puromycin |
Root
| Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens.
Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18.
|
for SaCas9 knockout, lentiviral expression of SaCas9 and gRNA scaffold
pXPR
|
| pPapi | | | Puromycin ; (located downstream of SaCas9-2A sequence) |
Root
| Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens.
Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18.
|
lentiviral expression of SaCas9 and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in Stable SpCas9 expressing cell lines for dual Cas9 "Big Papi" screens. Alt name pXPR207.
pXPR
|
| pLX_311-Cas9 | Cas9 (Other) | EF1a | Blasticidin |
Hahn
| Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.
Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026.
|
for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111
pXPR
|
| AAV_Efs_hSpCas9_NLS_FLAG-SV40 | humanized S. pyogenes Cas9 (Synthetic) | EFS | |
Yang
| Homology-mediated end joining-based targeted integration using CRISPR/Cas9.
Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
|
AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter
pAAV
Tag
/ Fusion Protein- Flag (C terminal on insert)
|
| Lenti_Efs_hSpCas9_NLS_FLAG-WPRE | humanized S. pyogenes Cas9 (Synthetic) | EFS | |
Yang
| Homology-mediated end joining-based targeted integration using CRISPR/Cas9.
Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76.
|
Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.
Lenti virus
Tag
/ Fusion Protein- Flag (C terminal on insert)
|
| lentiCRISPRv2 puro | P2A-puro | EF-1a | Puromycin |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
Variant of lentiCRISPRv2 that confers puromycin resistance
lentiCRISPRv2
|
| lentiCRISPRv2 hygro | P2A-hygro | EF-1a | Hygromycin |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
Variant of lentiCRISPRv2 that confers hygromycin resistance
lentiCRISPRv2
|
| lentiCRISPRv2 neo | P2a-neo | EF-1a | Neomycin (select with G418) |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
Variant of lentiCRISPRv2 that confers G418 resistance
lentiCRISPRv2
|
| lentiCRISPRv2 blast | P2a-blast | EF-1a | Blasticidin |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
Variant of lentiCRISPRv2 that confers blasticidin S resistance
lentiCRISPRv2
|
| px330-mcherry | | U6 | mCherry |
Li
| Correction of a genetic disease in mouse via use of CRISPR-Cas9.
Cell Stem Cell. 2013 Dec 5;13(6):659-62. doi: 10.1016/j.stem.2013.10.016.
|
Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA
pX330
|
| pCAGG>nls-hCas9-nls | humanized Cas9 | Chicken beta actin (CAG) | |
Bronner
| Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo.
Dev Biol. 2017 Dec 1;432(1):86-97. doi: 10.1016/j.ydbio.2017.08.036.
|
Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos
pCI
|
| pCAGG>nls-hCas9-nls-GFP | Cas9-GFP | Chicken beta actin (CAG) | |
Bronner
| Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo.
Dev Biol. 2017 Dec 1;432(1):86-97. doi: 10.1016/j.ydbio.2017.08.036.
|
Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter
pCI
|
| LentiCRISPRv2-mCherry | Cas9 (Synthetic), mCherry (Other) | EFS-NS, EFS-NS | mCherry |
Smogorzewska
|
LentiCRISPRv2-mCherry
(unpublished)
|
Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
LentiCRISPRv2
|
| CROPseq-Guide-EFS-SpCas9-P2A-EGFP | | EFS | |
Hewitt
|
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
(unpublished)
|
single-vector system with EGFP reporter for use in CROPseq knockout screens
CROPseq-Guide-Puro
Tag
/ Fusion Protein- EGFP (C terminal on backbone)
|
| ciCas9_pcDNA5 | ciCas9 | | Hygromycin |
Maly
| Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.
Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368.
|
Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
pcDNA5/FRT/TO
|
| e-ciCas9_pcDNA5 | e-ciCas9 | | Hygromycin |
Maly
| Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.
Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368.
|
Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
pcDNA5/FRT/TO
|
| eSpCas9(1.1)-T2A-Puro | | CBh | Puromycin |
Németh
|
eSpCas9(1.1)-T2A-Puro
(unpublished)
|
Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
eSpCas9(1.1)
Tags
/ Fusion Proteins- 3xFLAG (N terminal on insert)
- T2A-Puro (C terminal on insert)
|
| BPK3258 - human expression plasmid for eSpCas9(1.1) | hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
JDS246
|
| BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1 | hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG
JDS246
|
| BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9) | hSpCas9-Cluster1(N692A/M694A/Q695A/H698A) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A | hSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A | hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A | hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A | hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A | hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A | hSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW2993 - human expression plasmid for SpCas9 Cluster 2 | hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3645 - human expression plasmid for SpCas9 Cluster 3 | hSpCas9-Cluster3(T657A/G658A/W659A/R661A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A | hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3770 - human expression plasmid for SpCas9 Cluster 4 | hSpCas9-Cluster4(F491A/M495A/T496A/N497A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG
JDS246
|
| MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A | hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| BPK4387 - human expression plasmid for SpCas9 Cluster 5 | hSpCas9-Cluster5(K918A/V922A/R925A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG
JDS246
|
| BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A | hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A) (Synthetic) | CMV | |
Joung
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG
JDS246
|
| px459 VQR | SpCas9 VQR (Other) | | Puromycin |
Holland
|
AID Chapter plasmids
(unpublished)
|
sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
px459 v2
|
| px459 VRER | SpCas9 VRER (Other) | | Puromycin |
Holland
|
AID Chapter plasmids
(unpublished)
|
sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)
px459 v2
|
| p458 VQR | SpCas9 VQR (Other) | | EGFP |
Holland
|
px458 VQR
(unpublished)
|
Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
px458
|
| p458 VRER | SpCas9 VRER (Other) | | EGFP |
Holland
|
px458 VQR
(unpublished)
|
Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
px458
|
| px330 VRER | SpCas9 VRER (Other) | | |
Holland
|
px458 VQR
(unpublished)
|
Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
px330
|
| p330 VQR | SpCas9 VQR (Other) | | |
Holland
|
px458 VQR
(unpublished)
|
Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
px330
|
| px458 EQR | SpCas9 EQR (Other) | | EGFP |
Holland
|
px458 VQR
(unpublished)
|
Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
px458
|
| px459 EQR | SpCas9 EQR (Other) | | Puromycin |
Holland
|
px458 VQR
(unpublished)
|
Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
px459 v2
|
| px330 EQR | SpCas9 EQR (Other) | | |
Holland
|
px458 VQR
(unpublished)
|
Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
px330
|
| pLCRISPR-CMV | | | |
Manel
| Transmission of innate immune signaling by packaging of cGAMP in viral particles.
Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30.
|
Control lentivector expressing Cas9 and gRNA scaffold
pLCRISPR
|
| PCS2+Cas9-mSA | CAS9-MSA (Other) | SP6 | |
Rossant
| Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
|
Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos
PCS2+
|
| p3s-Cas9-HN | Cas9-WT (Other) | CMV | |
Kim
| Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides.
Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3.
|
Cas9-WT mammalian expression
p3s
Tag
/ Fusion Protein- HA (N terminal on backbone)
|
| p3s-eCas9-1.1 | eCas9-1.1 (Other) | CMV | |
Kim
| Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides.
Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3.
|
eCas9-1.1 mammalian expression
p3s
Tag
/ Fusion Protein- HA (N terminal on backbone)
|
| p3s-Cas9-HF1 | Cas9-HF1 (Other) | CMV | |
Kim
| Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides.
Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3.
|
Cas9-HF1 mammalian expression
p3s
Tag
/ Fusion Protein- HA (N terminal on backbone)
|
| pAAV-EFS-SpCas9 | Streptococcus pyogenes Cas9 (Synthetic) | EFS | |
Yasuda
| Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain.
Neuron. 2017 Oct 18. pii: S0896-6273(17)30933-9. doi: 10.1016/j.neuron.2017.10.004.
|
AAV vector expressing Myc-tagged SpCas9 under the EFS promoter
PX551
|
| lenti-SpCas9 puro | KpnI-XhoI-BsrGI-NheI (Synthetic) | | Puromycin |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.
lentiCRISPRv2 puro (Addgene #98290)
|
| lenti-SpCas9 hygro | KpnI-XhoI-BsrGI-NheI (Synthetic) | | Hygromycin |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
This lentiviral construct delivers hSpCas9 and hygromycin resistance.
lentiCRISPRv2 hygro (Addgene #98291)
|
| lenti-SpCas9 neo | KpnI-XhoI-BsrGI-NheI (Synthetic) | | Neomycin (select with G418) |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
This lentiviral construct delivers hSpCas9 and G418 resistance.
lentiCRISPRv2 neo (Addgene #98292)
|
| lenti-SpCas9 blast | KpnI-XhoI-BsrGI-NheI (Synthetic) | | Blasticidin |
Stringer
| A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma.
Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
|
This lentiviral construct delivers hSpCas9 and blasticidin S resistance.
lentiCRISPRv2 blast (Addgene #98293)
|
| pMB-SaCas9 | Cas9 nuclease (Other) | short EF1alpha | Hygromycin ; mouse CD4 |
McManus
| Dual gene activation and knockout screen reveals directional dependencies in genetic networks.
Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062.
|
SaCas9 expression vector
pBR322
|
| pSECB | | | Blasticidin |
Possemato
| Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs
Mol. Cell (2018) Volume 69, Issue 4, p610–621.e5
|
Express spCas9 with sgRNA, blast resistance
pSECB
|
| AAV-CMVc-Cas9 | Cas9 (Synthetic) | CMVc | |
Belmonte
| In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation.
Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7.
|
Expresses SpCas9 from the CMVc promoter in AAV backbone
AAV
|
| CROPseq-EFS-SpCas9-P2A-blast | | EFS | Blasticidin |
Hewitt
|
CROPseq plasmids
(unpublished)
|
single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
|
| CROPseq-EFS-HypaSpCas9-P2A-EGFP | HypaSpCas9 (Synthetic) | EFS | EGFP |
Hewitt
|
CROPseq plasmids
(unpublished)
|
single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
|
| CROPseq-EFS-HypaSpCas9-P2A-puro | HypaSpCas9 (Synthetic) | EFS | Puromycin |
Hewitt
|
CROPseq plasmids
(unpublished)
|
single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
CROPseq-Guide-EFS-SpCas9-P2A-puro
|
| pX551-CMV-SpCas9 | SpCas9 (Other) | | |
Hewitt
|
Alex Hewitt Lab plasmids
(unpublished)
|
AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
pAAV
|
| pX551-miniCMV-SpCas9 | SpCas9 (Other) | | |
Hewitt
|
Alex Hewitt Lab plasmids
(unpublished)
|
AAV plasmid expressing Cas9 under control of miniCMV promoter
pAAV
|
| pX551-miniCMV-SaCas9 | SaCas9 (Other) | | |
Hewitt
|
Alex Hewitt Lab plasmids
(unpublished)
|
AAV plasmid expressing SaCas9 under control of miniCMV promoter
pAAV
|
| pX551-miniCMV-CjCas9 | CjCas9 (Other) | | |
Hewitt
|
Alex Hewitt Lab plasmids
(unpublished)
|
AAV plasmid expressing CjCas9 under control of miniCMV promoter
pAAV
|
| pX551-miniCMV-AsCpf1 | AsCpf1 (Other) | | |
Hewitt
|
Alex Hewitt Lab plasmids
(unpublished)
|
AAV plasmid expressing AsCpf1 under control of miniCMV promoter
pAAV
|
| pX551-CMV-CjCas9 | CjCas9 (Other) | | |
Hewitt
|
Alex Hewitt Lab plasmids
(unpublished)
|
AAV plasmid expressing CjCas9 under control of CMV promoter
pAAV
|
| pX601 miniCMV-SaCas9-SpA-sgRNA scaffold | | | |
Hewitt
|
Alex Hewitt Lab plasmids
(unpublished)
|
Backbone vector for cloning in target sgRNA for use with SaCas9
pAAV
|
| pSP64T-hLbCpf1 | hLbCpf1 (Homo sapiens) | sp6 | |
Giraldez
| CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing.
Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
|
plasmid to carry out IVT of human codon optimized LbCpf1 (from Addgene; 69988)
pSP64T
|
| pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA | hSpCas9 (Other), rtTA, bGH polyA-SV40polyA | TRE, CAG | Puromycin |
Menéndez
|
Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locus.
(unpublished)
|
Expresses Cas9 upon induction with doxicycline.
N/A
|
| pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS | SaCas9 (Other) | CMV IE94 | |
Wolfe
| Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing.
Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x.
|
Expresses SaCas9 in mammalian cells
pCSDest2
|
| pTE3320 | hLbCpf1(RVR) | CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3314 | hFnCpf1(RVR) | CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3317 | hMbCpf1(RVR) | CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3316 | hFnCpf1(RR) | CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3319 | hMbCpf1(RR) | CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3330 | Lb crRNA, hLbCpf1(RVR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3327 | As crRNA, hAsCpf1(RVR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3331 | Fn crRNA, hFnCpf1(RVR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3334 | Mb crRNA, hMbCpf1(RVR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3329 | Lb crRNA, hLbCpf1(RR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3328 | As crRNA, hAsCpf1(RR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3333 | Fn crRNA, hFnCpf1(RR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.
pcDNA3.1
|
| pTE3336 | Mb crRNA, hMbCpf1(RR mutant) | human U6, CMV | Neomycin (select with G418) |
Welker
| Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants.
Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815.
|
Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.
pcDNA3.1
|
| pX-evoCas9 | Humanized S. pyogenes Cas9 | CBh | |
Cereseto
| A highly specific SpCas9 variant is identified by in vivo screening in yeast.
Nat Biotechnol. 2018 Jan 29. pii: nbt.4066. doi: 10.1038/nbt.4066.
|
Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells
PX330 (Addgene #42230)
|
| pST1374-Lb-Cpf1-NLS | LbCpf1 (Other) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses LbCpf1 in mammalian cells
Addgene 44758
|
| LentiV_Cas9_puro | Cas9 (Synthetic) | EFS promoter, EFS promoter | Puromycin |
Vakoc
| LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia.
Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011.
|
Lentiviral expression plasmid of spCas9 with puromycin resistance gene
LentiV_puro
|
| pX459V2.0-eSpCas9(1.1) | | | Puromycin |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations
pX459
|
| pX459V2.0-SpCas9-HF1 | | | Puromycin |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations
pX459
|
| pX459V2.0-HypaCas9 | | | Puromycin |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations
pX459
|
| pX459V2.0-SpCas9-HF4 | | | Puromycin |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations
pX459
|
| pX459V2.0-eSpCas9(1.1)+K810A | | | Puromycin |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations
pX459
|
| pX459V2.0-eSpCas9(1.1)+K855A | | | Puromycin |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations
pX459
|
| pX459V2.0-eSpCas9(1.1)+K810A+K855A | | | Puromycin |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations
pX459
|
| pX330-SpCas9-HF1 | | | |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations
pX330
|
| pX330-HypaCas9 | | | |
Miyaoka
| Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair
Nucleic Acids Research
|
pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations
pX330
|
| xCas9 3.7 | xCas9 3.7 (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9 3.7
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| xCas9 3.6 | xCas9 3.6 (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9 3.6
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| Myh6-Cas9 | Cas9 (Other) | alpha MHC | |
van Rooij
| Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption.
Circ Res. 2017 Oct 27;121(10):1168-1181. doi: 10.1161/CIRCRESAHA.116.310370. Epub 2017 Aug 29.
|
Cardiac-specific overexpression of Cas9
alpha MHC
|
| 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA | | | |
Lagor
| Somatic Editing of Ldlr With Adeno-Associated Viral-CRISPR Is an Efficient Tool for Atherosclerosis Research.
Arterioscler Thromb Vasc Biol. 2018 Jul 19. pii: ATVBAHA.118.311221. doi: 10.1161/ATVBAHA.118.311221.
|
Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA
pEMBL8
|
| hSyn1-AsCpf1(RR) | hAsCpf1(RR) (Homo sapiens) | hSyn1 | |
Zhang
| Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons
Nature Biomedical Engineering (Epub 09 April 2018)
|
AAV vector expressing AsCpf1 (RR variant)
Custom
|
| pLenti-LbCpf1-U6_FlipArray | U6-FlipArray (Synthetic) | U6 | Puromycin |
Chen
| Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion.
Nat Commun. 2018 May 15;9(1):1903. doi: 10.1038/s41467-018-04158-z.
|
Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.
pY109
|
| pMIA3 | | EF1a | |
Foo
| A Roadmap for Human Liver Differentiation from Pluripotent Stem Cells.
Cell Rep. 2018 Feb 20;22(8):2190-2205. doi: 10.1016/j.celrep.2018.01.087.
|
Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
px330 (addgene #42230)
Tags
/ Fusion Proteins- mRuby2 (N terminal on insert)
- mtp53dn (N terminal on insert)
|
| pCas9-HE | HDR-Enhancer domain of human CtIP protein (Homo sapiens) | | Neomycin (select with G418) |
Concordet
| CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair.
Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
|
Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein
hCas9 Addgene ID 41815
RBBP8 COM1, CTIP, JWDS, RIM, SAE2, SCKL2
|
| pCas9-Geminin | Geminin (Homo sapiens) | | Neomycin (select with G418) |
Concordet
| CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair.
Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
|
Expresses Cas9 fusion to Geminin degron domain
hCas9 Addgene ID 41815
GMNN Gem, MGORS6
|
| pCas9-HE-Geminin | HE-Geminin | | Neomycin (select with G418) |
Concordet
| CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair.
Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
|
Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron
hCas9 Addgene ID 41815
|
| pCas9-CtIP | CtIP (Homo sapiens) | | Neomycin (select with G418) |
Concordet
| CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair.
Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7.
|
Expresses Cas9 fusion to human CtIP protein
hCas9 Addgene ID 41815
RBBP8 COM1, CTIP, JWDS, RIM, SAE2, SCKL2
|
| pX458_Ruby | hSpCas9 | | |
Hublitz
| Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling.
Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483.
|
Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA
PX458
|
| pX330.puro | | CBh | Puromycin |
Dias
|
HuR (ELAVL1) silencing sensitizes triple-negative breast cancer to glutaminase-targeted therapy
(unpublished)
|
CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pX330.orup | | CBh | Puromycin |
Dias
|
HuR (ELAVL1) silencing sensitizes triple-negative breast cancer to glutaminase-targeted therapy
(unpublished)
|
CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA | St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens) | LP1B, hU6 | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
pX602 (Plasmid #61593)
|
| MSP1594_2x_NLS | St1Cas9 LMD-9 (Homo sapiens), None (Synthetic) | CAG | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)
MSP1594 (Plasmid #65775)
|
| U6_sgRNA_CAG_hSt1Cas9_LMD9 | St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens) | CAG, hU6 | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.
MSP1594_2x_NLS (Plasmid #110625)
|
| St1Cas9_LMD-9_sgRNA_pUC19 | Empty St1Cas9 LMD-9 sgRNA backbone (Homo sapiens) | hU6 | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).
pUC19
|
| pLenti-Cas9-P2A-Puro | Cas9 (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-VQR-P2A-Puro | Cas9-VQR (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-VRER-P2A-Puro | Cas9-VRER (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-HF1-P2A-Puro | Cas9-HF1 (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-VQRRA-P2A-Puro | Cas9-VQRRA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-VRERRA-P2A-Puro | Cas9-VRERRA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-HF1RA-P2A-Puro | Cas9-HF1RA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-VQR-PGK-Puro | Cas9-VQR (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-VRER-PGK-Puro | Cas9-VRER (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-HF1-PGK-Puro | Cas9-HF1 (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-VQRRA-PGK-Puro | Cas9-VQRRA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-VRERRA-PGK-Puro | Cas9-VRERRA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-HF1RA-PGK-Puro | Cas9-HF1RA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-VQR-P2A-GFP-PGK-Puro | Cas9-VQR (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-VRER-P2A-GFP-PGK-Puro | Cas9-VRER (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-HF1-P2A-GFP-PGK-Puro | Cas9-HF1 (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (C terminal on insert)
|
| pLenti-VQRRA-P2A-GFP-PGK-Puro | Cas9-VQRRA (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-VRERRA-P2A-GFP-PGK-Puro | Cas9-VRERRA (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-HF1RA-P2A-GFP-PGK-Puro | Cas9-HF1RA (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pLenti-xCas9RA-P2A-Puro | xCas9(3.7)RA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- FLAG (N terminal on insert)
|
| pEJS654 All-in-One AAV-sgRNA-hNmeCas9 | sgRNA scaffold, Human-codon-optimized NmeCas9 (Other) | U6, U1a | |
Sontheimer
| All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo.
Genome Biol. 2018 Sep 19;19(1):137. doi: 10.1186/s13059-018-1515-0.
|
Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
PX551-Plasmid #60957
|
| pX458-DsRed2 | DsRed2 (Synthetic) | | |
Liang
| Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion.
J Biotechnol. 2018 Jun 7;281:11-20. doi: 10.1016/j.jbiotec.2018.06.308.
|
Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2
pX458
|
| pX458-ECFP | ECFP (Synthetic) | | |
Liang
| Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion.
J Biotechnol. 2018 Jun 7;281:11-20. doi: 10.1016/j.jbiotec.2018.06.308.
|
Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP
pSpCas9(BB)-2A-GFP (PX458)
|
| CMV-SpyCas9 | SpyCas9 | | |
Niopek
| Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9.
Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9.
|
SpyCas9 expression in mammalian cells (CMV promoter driven)
pcDNA3.1
|
| pAAV-CMV-SpyCas9 | SpyCas9 | | |
Niopek
| Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9.
Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9.
|
AAV vector for expression of SpyCas9 in mammalian cells
ssAAV vector
|
| pX330 spCas9-mSA | CAS9-MSA (Other) | | |
Rossant
| Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
|
pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines
pX330
|
| pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA | SaCas9 | Cytomegalo Virus(CMV) | |
Ploski
| The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase.
Front. Mol. Neurosci. (2018) 13
|
SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
AAV
NEWENTRY
|
| pJEP313-pAAV-CMV-SaCas9-DIO-pA | SaCas9 | Cytomegalo Virus(CMV) | |
Ploski
| The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase.
Front. Mol. Neurosci. (2018) 13
|
A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
AAV
NEWENTRY
|
| p3s-Sniper-Cas9 | Sniper-Cas9 | CMV | |
Lee
| Directed evolution of CRISPR-Cas9 to increase its specificity.
Nat Commun. 2018 Aug 6;9(1):3048. doi: 10.1038/s41467-018-05477-x.
|
Expresses Sniper-Cas9 with CMV promoter
pCDNA3.1
|
| pSpCTRE-CD4 | SpCas9 (Homo sapiens) | | CD4 |
Rudin
| Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics
Nature Cancer 1, 359–369 (2020)
|
Dox-inducible Cas9 expression with a CD4 selection marker
pLVX-TetOne-Puro
|
| pSH231-EFS-Cas9-BlastR | Cas9-T2A-BlastR (Other) | EF1a | Blasticidin |
Monnat
| New human chromosomal safe harbor sites for genome engineering with CRISPR/Cas9, TAL effector and homing endonucleases
bioRxiv 396390
|
Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette
Piggybac
|
| pU6-SacB-Cas9-T2A-mCherry_(BbsI) | Cas9 (Other), SacB (Other) | CBh, U6 | T2A-mCherry |
Failor
|
CRISPR-Cas9 system with SacB toxin Positive Selection
(unpublished)
|
Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
pX330
|
| pBC2101-reci | reci_wtCas9 with SV40 NLS | | |
Savage
| Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
|
U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS
Lenti
|
| pBC2102-reci | reci_wtCas9 without NLS | U6 Promoter | |
Savage
| Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
|
U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS
Lenti
|
| pBC2103-reci | reci_arCas9 without NLS | | |
Savage
| Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.
Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
|
U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS
Lenti
|
| eSpCas9(1.1)-Puro | eSpCas9(1.1) (Synthetic) | U6 | Puromycin |
Leyns
| Loss of Emp2 compromises cardiogenic differentiation in mouse embryonic stem cells.
Biochem Biophys Res Commun. 2019 Feb 14. pii: S0006-291X(19)30235-9. doi: 10.1016/j.bbrc.2019.02.048.
|
The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
pSpCas9(BB)-2A-Puro
|
| pScCas9 | Streptococcus canis Cas9 (Other) | EF-1α | |
Jacobson
| Minimal PAM specificity of a highly similar SpCas9 ortholog.
Sci Adv. 2018 Oct 24;4(10):eaau0766. doi: 10.1126/sciadv.aau0766. eCollection 2018 Oct.
|
Mammalian expression vector encoding WT ScCas9
OG3569
|
| pX330-SpCas9-NG | SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) | | |
Nureki
| Engineered CRISPR-Cas9 nuclease with expanded targeting space.
Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129.
|
Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.
pX330
|
| pLex_Cas9 | Cas9-P2A-NLS-BASTR (Other) | | Blasticidin |
Khavari
| Coupled Single-Cell CRISPR Screening and Epigenomic Profiling Reveals Causal Gene Regulatory Networks.
Cell. 2018 Dec 11. pii: S0092-8674(18)31514-9. doi: 10.1016/j.cell.2018.11.022.
|
pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.
pLex
|
| pLX311-Cas9 | Cas9 (Other) | EF1alpha | Blasticidin |
Hahn
| Mutational processes shape the landscape of TP53 mutations in human cancer.
Nat Genet. 2018 Oct;50(10):1381-1387. doi: 10.1038/s41588-018-0204-y.
|
Constitutive expression of Cas9
pLX311
|
| pSpCas9(BB)-2A-Blast | | | Blasticidin |
Takemaru
|
pSpCas9(BB)-2A-Blast
(unpublished)
|
Used for expression of sgRNA in mammalian cells
pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
|
| pSpCas9(BB)-T2A-HygR | hSpCas9-T2A-HygR (Homo sapiens) | CAG and U6 | Hygromycin |
Ferrer
| Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes.
Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 10.1038/s41588-019-0457-0.
|
SpCas9 expression vector
pX458
Tag
/ Fusion Protein- 3XFLAG (N terminal on insert)
|
| pCAG-SpCas9-2A-GFP-noITR | Cas9 (Other) | | |
Denham
| A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity.
Front Cell Dev Biol. 2018 May 15;6:54. doi: 10.3389/fcell.2018.00054. eCollection 2018.
|
Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression
pSpCas9(BB)-2A-GFP (PX458)
|
| HF-PX459 (V2) | hSpCas9-HF1-2A-Puro V2.0 (Synthetic) | Cbh | Puromycin |
McGrew
| High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells.
Sci Rep. 2018 Oct 11;8(1):15126. doi: 10.1038/s41598-018-33244-x.
|
Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
PX459
|
| Lenti_SaCRISPR_GFP | | U6 | GFP |
Vakoc
| A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence.
Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
|
To clone sgRNA for SaCas9
Lenti_SaCRISPR_GFP
|
| Nme2Cas9_pCDest | Nme2Cas9 (Other) | CMV | |
Sontheimer
| A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing.
Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 10.1016/j.molcel.2018.12.003.
|
Nme2Cas9 CMV-driven mammalian expression plasmid
pCDest
|
| Nme2Cas9_AAV | Nme2Cas9 (Other) | U1a | |
Sontheimer
| A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing.
Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 10.1016/j.molcel.2018.12.003.
|
All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette
AAV
|
| AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E) | N-terminal fragment of SpyCas9 (Other) | | |
Niopek
| Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins.
Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271.
|
AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold
pZac2.1
Tags
/ Fusion Proteins- SV40-NLS (N terminal on insert)
- split-intein (C terminal on insert)
|
| EF1a-Cas9-2XNES-2ERT2 | Cas9, NES and ERT2 (Synthetic) | EF1α | Zeocin |
Wang
| HIT-Cas9: A CRISPR/Cas9 Genome-Editing Device under Tight and Effective Drug Control.
Mol Ther Nucleic Acids. 2018 Dec 7;13:208-219. doi: 10.1016/j.omtn.2018.08.022. Epub 2018 Sep 1.
|
Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells
pRRL.sin-18.ppy.
|
| pAAV.pU1a-SpCas9 | SpCas9 (Synthetic) | U1a | |
Gao
| Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice.
Nat Biotechnol. 2018 Oct;36(9):839-842. doi: 10.1038/nbt.4219. Epub 2018 Aug 13.
|
Expresses codon-optimized SpCas9 in mammalian cells.
pAAV
|
| pCF204 | Cas9 (Other) | U6 | Puromycin |
Savage
| CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification.
Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052.
|
U6-sgRNA EFS-Cas9-wt-P2A-Puro
pU6
|
| pCF226 | cas9 (Other) | EF-1a core promoter | Puromycin |
Savage
| CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification.
Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052.
|
EFS-Cas9-wt-P2A-Puro
pEFS
|
| pLenti spCas9 T2A iRFP670 P2A puro | spCas9, iRFP670 | | Puromycin |
Gaudin
|
Lenti spCas9 T2A iRFP670 P2A puro
(unpublished)
|
The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
pLenti
NEWENTRY
|
| pLenti spCas9 T2A mNeonGreen P2A puro | spCas9, mNeonGreen | | Puromycin |
Gaudin
|
Lenti spCas9 T2A iRFP670 P2A puro
(unpublished)
|
The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
pLenti
NEWENTRY
|
| pLenti spCas9 T2A TagRFP-T P2A puro | spCas9, TagRFP-T | | |
Gaudin
|
Lenti spCas9 T2A iRFP670 P2A puro
(unpublished)
|
The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
pLenti
|
| pSaCas9-1xms2-2x3’UTR | SaCas9-MS2-HBB 3' UTR (Other) | | |
Lu
| Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing.
Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093.
|
For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
|
| pSaCas9-1xPP7-2x3'UTR | SaCas9-PP7-HBB 3' UTR (Other) | | |
Lu
| Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing.
Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093.
|
For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
|
| PCS2+ Cas9 | Cas9 (Synthetic) | SP6 | |
Rossant
| Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos.
Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166.
|
For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos
PCS2+
|
| pRC10-EFS-huLbCpf1-2A-Blast-WPRE | human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium, (Synthetic) | EFS-NS | Blasticidin |
Chen
| In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens.
Nat Methods. 2019 May;16(5):405-408. doi: 10.1038/s41592-019-0371-5. Epub 2019 Apr 8.
|
Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.
pFUGW
|
| KA2058_pX330A-G2P | | | Puromycin |
Schöler
| Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
|
Expression of SpCas9 and sgRNA
pX330
|
| KA2601_eSpCas9-G2P | | | Puromycin |
Schöler
| Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
|
Expression of eSpCas9(1.1) and sgRNA
eSpCas9(1.1)
|
| KA2706_eSpCas9-PGKPacdtkCh | | | Puromycin |
Schöler
| Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
|
Expression of eSpCas9(1.1) and sgRNA
eSpCas9(1.1)
|
| KA2963_eSpCas9-PGKHygdtkCh | | | Hygromycin |
Schöler
| Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
|
Expression of eSpCas9(1.1) and sgRNA
eSpCas9(1.1)
|
| pKH-1699 | SpCas9-HF1 | CMV | |
Welker
| A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs.
Nucleic Acids Res. 2021 Jan 15. pii: 6101605. doi: 10.1093/nar/gkaa1220.
|
Mammalian SpCas9-HF1 expression
p15A
|
| pX330-NL-DHFR-SpCas9 | NL-DHFR-SpCas9 (Other) | Cbh | |
Choudhary
| A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR-Cas9.
Angew Chem Int Ed Engl. 2019 May 6;58(19):6285-6289. doi: 10.1002/anie.201900788. Epub 2019 Apr 2.
|
Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells
pX330
|
| pX330-NLC-DHFR-SpCas9 | NLC-DHFR-SpCas9 (Other) | Cbh | |
Choudhary
| A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR-Cas9.
Angew Chem Int Ed Engl. 2019 May 6;58(19):6285-6289. doi: 10.1002/anie.201900788. Epub 2019 Apr 2.
|
Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells
pX330
|
| Lenti-Cas9-gRNA-GFP | | | GFP |
Sheltzer
| Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9.
Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100.
|
3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone
LRG2.1 (Addgene #108098)
|
| Lenti-Cas9-gRNA-TagBFP2 | | | TagBFP2 |
Sheltzer
| Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9.
Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100.
|
3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone
Lenti-Cas9-gRNA-GFP (Addgene #124770)
|
| pAAV-FLEX-SaCas9-U6-sgRNA | gRNA scaffold (Mus musculus) | | |
Zweifel
| Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System.
Cell Rep. 2020 Mar 24;30(12):4303-4316.e6. doi: 10.1016/j.celrep.2020.02.092.
|
Vector for Cre-dependent expression of SaCas9
pX601
|
| pMSCV-Cas9-2A-GFP-sgRNA | Cas9 (Synthetic) | MSCV | |
Lodish
| Efficient CRISPR-Cas9 mediated gene disruption in primary erythroid progenitor cells.
Haematologica. 2016 Jun;101(6):e216-9. doi: 10.3324/haematol.2015.135723. Epub 2016 Mar 11.
|
Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector
pMCSV
|
| pAAVS1-tet-iCas9-BFP2 | AAVS1-TET-ON 3G-SpCas9-P2A-BFP2 (Homo sapiens) | U6 | Neomycin (select with G418) |
Moffat
| Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.
Cell Rep. 2019 Apr 9;27(2):599-615.e12. doi: 10.1016/j.celrep.2019.02.041.
|
all-in-one-inducible Cas9
pAAVS1
|
| LentiV_Cas9_Blast | Cas9 (Synthetic) | EFS promoter, EFS promoter | Blasticidin |
Vakoc
| Salt-Inducible Kinase inhibition suppresses acute myeloid leukemia progression in vivo.
Blood. 2019 Nov 1. pii: 422697. doi: 10.1182/blood.2019001576.
|
Lentiviral expression of spCas9 with blasticidin resistance gene
LentiV_Blast
|
| PB_tre_Cas9 | humanized S. pyrogenes Cas9, Hygromycin resistance | TRE, EF1 alpha | Hygromycin |
Calabrese
| A piggyBac-based toolkit for inducible genome editing in mammalian cells.
RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118.
|
PiggyBac cargo vector expressing doxycycline inducible Cas9
piggyBac cargo vector
|
| pcDNA-Cas9-T2A-STOP-TdT | Cas9-T2A-STOP-TdT (Homo sapiens) | pCMV | Hygromycin |
Liu
| DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cells
bioRxiv (2019)
|
Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)
pcDNA3.1
DNTT TDT
|
| pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations) | WT SpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-eSpCas9 (without sgRNA; with silent mutations) | eSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations) | SpCas9-HF1 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations) | HypaSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations) | evoSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations) | HeFSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| B-SpCas9 | B-SpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
pX330-like (without U6-sgRNA coding sequence)
|
| B-eSpCas9 | B-eSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
pX330-like (without U6-sgRNA coding sequence)
|
| B-SpCas9-HF1 | B-SpCas9-HF1 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
pX330-like (without U6-sgRNA coding sequence)
|
| B-HypaSpCas9 | B-HypaSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
pX330-like (without U6-sgRNA coding sequence)
|
| B-evoSpCas9 | B-evoSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.
pX330-like (without U6-sgRNA coding sequence)
|
| B-HeFSpCas9 | B-HeFSpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.
pX330-like (without U6-sgRNA coding sequence)
|
| eSpCas9-plus | eSpCas9-plus (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
pX330-like (without U6-sgRNA coding sequence)
|
| SpCas9-HF1-plus | SpCas9-HF1-plus (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations) | Sniper SpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations) | HiFi SpCas9 (Other) | CBh | |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
pX330-like (without U6-sgRNA coding sequence)
|
| TLCV2-LoxP | Cas9-2A-eGFP (Homo sapiens) | U6 | Puromycin |
Karpf
|
Karpf Lab plasmids
(unpublished)
|
LentiCRISPR v2 was modified into an all-in-one dox inducible system.
lentiCRISPR v2
|
| pFUGW Cas9-BFP | Cas9 (Other) | SVVF | |
Zhuang
| Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization.
Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13.
|
Cas9 expression
pFUGW
|
| LentiAsCpf1-3xMYC-blast | AsCpf1 (Synthetic) | | Blasticidin |
Draetta
| Pooled library screening with multiplexed Cpf1 library.
Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x.
|
Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal
pFUGW
|
| pSpCas9(BB)-2A-Neo | Empty backbone | | Neomycin (select with G418) |
Takemaru
|
Used for expression of sgRNA in mammalian cells
(unpublished)
|
Used for expression of sgRNA in mammalian cells
pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
|
| pSpCas9(BB)-2A-Hygro | | | Hygromycin |
Takemaru
|
Used for expression of sgRNA in mammalian cells
(unpublished)
|
Used for expression of sgRNA in mammalian cells
pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
|
| pX330GFP-hU6-gRNA-hSpCas9 | GFP, gRNA and hSpCas9 | | |
Tucker
|
CRISPR-Cas9-Based Genome Editing of Human Induced Pluripotent Stem Cells.
(unpublished)
|
GFP positive gRNA/hSpCas9 construct
pX330
|
| pCDNA3.3 CAS9 IRES-Dsred | FnCas9 IRES-Dsred (Synthetic) | | |
Laur
|
Emory Custom Cloning Core Plasmids - Oskar Laur
(unpublished)
|
expression of FnCas9 and DsRed
pCDNA3.3 CAS9
|
| p1195-pssAAV.U1a.hNme2Cas9 | codon-optimized Nme2Cas9 (Other) | U1a | |
Sontheimer
| Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins.
RNA. 2019 Aug 22. pii: rna.071704.119. doi: 10.1261/rna.071704.119.
|
AAV vector expressing Nme2Cas9
AAV
|
| pCas9/VRQR-sgRNA (BbsI) | | | Puromycin ; mCherry for FACS enrichment |
Ikonen
| An efficient auxin-inducible degron system with low basal degradation in human cells.
Nat Methods. 2019 Aug 26. pii: 10.1038/s41592-019-0512-x. doi: 10.1038/s41592-019-0512-x.
|
Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM
pGL3-basic
|
| PX458-3xHA-SpCas9 | 3xHA-NLS-SpCas9 (Synthetic) | Cbh | |
Chakraborty
| Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing.
Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30.
|
3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA
PX458
|
| PX458-3xHA-FnCas9 | 3xHA-NLS-FnCas9 (Synthetic) | Cbh | |
Chakraborty
| Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing.
Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30.
|
3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA
PX458
|
| pX458M-53BP1-DN1S | SpCas9-53BP1-DN1S (Homo sapiens) | CBH | |
Malik
| CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites.
Nat Commun. 2019 Jun 28;10(1):2866. doi: 10.1038/s41467-019-10735-7.
|
Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.
PX458M
TP53BP1 53BP1, TDRD30, p202, p53BP1
|
| pSaCas9-sgRNA-Tetra-com vector | vector for com modified sgRNA (Synthetic) | | |
Lu
| Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing.
Nucleic Acids Res. 2019 Jul 12. pii: 5531787. doi: 10.1093/nar/gkz605.
|
For expressing gRNA, whose Tetraloop is replaced with a com aptamer
pX601
|
| pCAG-T3-SpCAS9-NG-pA | Codon optimized SpCas9-NG (Synthetic) | | |
Fujii
| Generation of genetically modified mice using SpCas9-NG engineered nuclease.
Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.1038/s41598-019-49394-5.
|
Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
pCAGGS
|
| pCAG-T3-eSpCAS9-NG-pA | Codon optimized eSpCas9-NG (Synthetic) | | |
Fujii
| Generation of genetically modified mice using SpCas9-NG engineered nuclease.
Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.1038/s41598-019-49394-5.
|
Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
pCAGGS
|
| pCAG-T3-HypaCAS9-pA | Codon optimized HypaCas9 (Synthetic) | | |
Fujii
| High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes.
Commun Biol. 2019 Oct 10;2:371. doi: 10.1038/s42003-019-0627-8. eCollection 2019.
|
Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
pCAGGS
|
| pORANGE Cloning template vector | SpCas9 (Other) | U6 and Cbh | |
MacGillavry
| ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.
PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665.
|
Cloning template form ORANGE method based knock-in constructs
pORANGE
Tag
/ Fusion Protein- HA (N terminal on insert)
|
| pFUGW SpCas9 | SpCas9 (Other) | hUBC | |
MacGillavry
| ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.
PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665.
|
lentiviral expression of SpCas9 under hUBC promotor
pFUGW
|
| pCAG-T3-st1CAS9-pA | Codon optimized st1Cas9 (Synthetic) | | |
Fujii
| Zygote-mediated generation of genome-modified mice using Streptococcus thermophilus 1-derived CRISPR/Cas system.
Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30988-3. doi: 10.1016/j.bbrc.2016.06.070.
|
Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
pCAG-T3-hCAS9-pA
|
| pCAG-T3-CjCAS9-pA | Codon optimized CjCas9 (Synthetic) | CAG, T3 | |
Fujii
| Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas.
Int J Mol Sci. 2017 Oct 31;18(11). pii: ijms18112286. doi: 10.3390/ijms18112286.
|
Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
pCAG-T3-hCAS9-pA
|
| pAWp63-clone32 (Opti-SpCas9) | Opti-SpCas9 (Synthetic) | EFS | Zeocin |
Wong
| Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9.
Nat Methods. 2019 Aug;16(8):722-730. doi: 10.1038/s41592-019-0473-0. Epub 2019 Jul 15.
|
Expresses Opti-SpCas9 in mammalian cells
pFUGW
|
| pAWp63-clone3-12 (OptiHF-SpCas9) | OptiHF-SpCas9 (Synthetic) | EFS | Zeocin |
Wong
| Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9.
Nat Methods. 2019 Aug;16(8):722-730. doi: 10.1038/s41592-019-0473-0. Epub 2019 Jul 15.
|
Expresses OptiHF-SpCas9 in mammalian cells
pFUGW
|
| pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT | Cas9-ODD-T2A-TdT (Homo sapiens) | 4xHRE_YB TATA | Blasticidin |
Liu
| DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cells
bioRxiv (2019)
|
Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.
pcDNA3.1
DNTT TDT
|
| LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS | humanized SpCas9 (Synthetic) | | |
Newgard
|
Unpublished pMVP and pMAGIC plasmids
(unpublished)
|
pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
pDONR221 P4r-P3r
|
| SiC-V1 | SpCas9 (Synthetic) | | |
Neelamegham
| Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing.
Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006.
|
SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.
LentiCRISPR v2
|
| BII-gR-PtW-eSpCas9 | eSpCas9 (Other) | CMV enhancer and hEf1a (CpG free) | Puromycin |
Madison
|
Madison Lab - manuscript in preparation
(unpublished)
|
Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
BII-gR-PtW-EspCas9
Tag
/ Fusion Protein- HA-tagged eSpCas9 (N terminal on insert)
|
| BII-gR-BtW-eSpCas9 | eSpCas9 (Other) | CMV enhancer and hEf1a (CpG free) | Blasticidin |
Madison
|
Madison Lab - manuscript in preparation
(unpublished)
|
Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
BII-gR-BtW-EspCas9
Tag
/ Fusion Protein- Flag-tagged eSpCas9 (N terminal on insert)
|
| BII-ChBtW-eSpCas9 | eSpCas9 (Other) | CMV enhancer and hEf1a (CpG free) | Blasticidin |
Madison
|
Madison Lab - manuscript in preparation
(unpublished)
|
Piggybac vector for constitutive expression
BII-ChBtW
Tag
/ Fusion Protein- Flag (N terminal on backbone)
|
| BII-ChPtW-eSpCas9 | eSpCas9 (Other) | CMV enhancer and hEf1a (CpG free) | Puromycin |
Madison
|
Madison Lab - manuscript in preparation
(unpublished)
|
Piggybac vector for constitutive expression
BII-ChPtW
Tag
/ Fusion Protein- HA (N terminal on insert)
|
| pXPR_212 | control guide | | Puromycin |
Doench
| Genetic screens in isogenic mammalian cell lines without single cell cloning.
Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6.
|
U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance
lentiCRISPRv2
|
| pXPR_124 | SpyoCas9 (Other) | | |
Doench
| Genetic screens in isogenic mammalian cell lines without single cell cloning.
Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6.
|
EF1a promoter expresses SpyoCas9 and P2A provides EGFP
pLV
|
| pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426) | human codon optimized St3Cas9 (Synthetic) | CAG | |
Kleinstiver
| Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer.
Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)
pCAG (Addgene #11179)
Tag
/ Fusion Protein- NLS(SV40)-3xFLAG (C terminal on backbone)
|
| pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579) | human codon optimized CjeCas9 (Synthetic) | CAG | |
Kleinstiver
| Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer.
Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)
pCAG (Addgene #11179)
Tag
/ Fusion Protein- NLS(SV40)-3xFLAG (C terminal on backbone)
|
| pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409) | human codon optimized NmeCas9 (Synthetic) | CAG | |
Kleinstiver
| Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer.
Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)
pCAG (Addgene #11179)
Tag
/ Fusion Protein- NLS(SV40)-3xFLAG (C terminal on backbone)
|
| HP138-puro | Contains Cas9 (Other) | | Puromycin |
Cheeseman
|
Cheeseman Lab Plasmids
(unpublished)
|
Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.
unknown
|
| HP138-neo | Contains Cas9 (Other) | | Neomycin (select with G418) |
Cheeseman
|
Cheeseman Lab Plasmids
(unpublished)
|
Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection
unknown
|
| pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582) | human codon optimized Nme2Cas9 (Synthetic) | CAG | |
Kleinstiver
| Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer.
Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag
pCAG (Addgene plasmid #11179)
Tag
/ Fusion Protein- NLS(SV40)-3xFLAG (C terminal on insert)
|
| px458_Conc2 | | U6 | GFP |
Stange
| Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid
J. Vis. Exp. 2020, e60704
|
Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP
pSpCas9(BB)-2A-GFP (PX458)
|
| px458_Conc3 | | U6 | GFP |
Stange
| Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid
J. Vis. Exp. 2020, e60704
|
Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP
pSpCas9(BB)-2A-GFP (PX458)
|
| px459V2.0_Conc2 | | U6 | Puromycin |
Stange
| Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid
J. Vis. Exp. 2020, e60704
|
Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro
pSpCas9(BB)-2A-Puro (PX459) V2.0
|
| px459V2.0_Conc3 | | U6 | Puromycin |
Stange
| Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid
J. Vis. Exp. 2020, e60704
|
Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro
pSpCas9(BB)-2A-Puro (PX459) V2.0
|
| pCAG-CFP-SaCas9-HF (without sgRNA) | Staphylococcus aureus Cas9 nucleases with high genome-wide specificity | | |
Zheng
| Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity.
Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1906843116. doi: 10.1073/pnas.1906843116.
|
Human expression vector for SaCas9-HF variant.
pCAG-CFP
|
| pL40C_PGKintron_Cas9_Green | Cas9-P2A-mNeonGreen (Other) | hPGK promoter with beta-globin intron | |
Bornhauser
| The Leukemogenic TCF3-HLF Complex Rewires Enhancers Driving Cellular Identity and Self-Renewal Conferring EP300 Vulnerability.
Cancer Cell. 2019 Dec 9;36(6):630-644.e9. doi: 10.1016/j.ccell.2019.10.004. Epub 2019 Nov 14.
|
Lentiviral vector coding Cas9 and mNeonGreen
L40C
|
| pU6-(BsaI)_CBh-Cas9 | humanized S. pyogenes Cas9 (Other) | CBh | |
Shaul
| Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing.
Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584.
|
Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9
pX330
|
| pU6-(BsaI)_CBh-UN-Cas9 | humanized S. pyogenes Cas9 (Other) | CBh | |
Shaul
| Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing.
Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584.
|
Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
pX330
|
| pU6-(BsaI)_CBh-Cas9-T2A-mCherry | humanized S. pyogenes Cas9 (Other) | CBh | |
Shaul
| Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing.
Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584.
|
Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide
pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230)
|
| pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry | humanized S. pyogenes Cas9 (Other) | CBh | |
Shaul
| Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing.
Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584.
|
Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide
pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230)
|
| pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry | humanized S. pyogenes Cas9 (Other) | CBh | |
Shaul
| Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing.
Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584.
|
Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide
pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230)
|
| epiCRISPR | | | |
Wang
| An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells.
Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y.
|
Expresses SpCas9, and cloning backbone for sgRNA
epiCRISPR plasmid
|
| epiCRISPRe | | | |
Wang
| An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells.
Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y.
|
Expresses SpCas9, and cloning backbone for sgRNA
epiCRISPR plasmid
|
| pAAV-CMV-SauriCas9 | | | |
Wang
| A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope.
PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.
|
Expresses SauriCas9, and cloning backbone for sgRNA
AAV2 ITR vector
|
| pAAV-CMV-SauriCas9-Puro | | | Puromycin |
Wang
| A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope.
PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.
|
Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA
AAV2 ITR vector
|
| pAAV-CMV-SauriCas9-KKH-Puro | | | Puromycin |
Wang
| A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope.
PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.
|
Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA
AAV2 ITR vector
|
| pAAV-CMV-Sa-SauriCas9 | | | |
Wang
| A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope.
PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.
|
Expresses Sa-SauriCas9, and cloning backbone for sgRNA
AAV2 ITR vector
|
| KO plasmid | | | |
Wang
| krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells.
J Biol Eng. 2019 Apr 24;13:35. doi: 10.1186/s13036-019-0150-y. eCollection 2019.
|
Expresses SpCas9, and cloning backbone for sgRNA
epiCRISPR plasmid
|
| pspCas9-3'UTR-ST2-com-vector | SpCas9 and sgRNA scaffold (Other) | | |
Lu
|
Lentiviral capsid delivery of SpCas9 RNP
(unpublished)
|
Expresses SpCas9 and sgRNA Scaffold
pX601
|
| U6_sgRNA_CAG_hSt1Cas9_CNRZ1066 | St1Cas9 LMD-9:CNRZ_1066 Chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens) | CAG, hU6 | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.
MSP1594_2x_NLS (Plasmid #110625)
|
| U6_sgRNA_CAG_hSt1Cas9_LMG18311 | St1Cas9 LMD9:LMG18311 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens) | CAG, hU6 | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
MSP1594_2x_NLS (Plasmid #110625)
|
| U6_sgRNA_CAG_hSt1Cas9_TH1477 | St1Cas9 LMD-9:TH1477 Chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens) | CAG, hU6 | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
MSP1594_2x_NLS (Plasmid #110625)
|
| U6_sgRNA_CAG_hSt1Cas9_MTH17CL396 | St1Cas9 LMD-9:MTH17CL396 Chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens) | CAG, hU6 | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.
MSP1594_2x_NLS (Plasmid #110625)
|
| pCMV-Cas9-NRRH | Cas9-NRRH (Other) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses Cas9-NRRH in mammalian cells
pBR322 CMV
|
| pCMV-Cas9-NRTH | Cas9-NRTH (Other) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses Cas9-NRTH in mammalian cells
pBR322 CMV
|
| pCMV-Cas9-NRCH | Cas9-NRCH (Other) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses Cas9-NRCH in mammalian cells
pBR322 CMV
|
| pCF820_U6-sgRNA-EF1a-mCherry2_lenti | SpyCas9 GFP sgRNA | | mCherry2 |
Doudna
| Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes.
Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117.
|
Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP
pCF525
|
| pCF823_EFS-Cas9-PuroR_lenti | SpyCas9 | | Puromycin |
Doudna
| Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes.
Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117.
|
Lenti expression of SpyCas9 with Puro selection
pCF525
|
| pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti | SauCas9 GFP sgRNA | | mCherry2 |
Doudna
| Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes.
Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117.
|
Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP
pCF525
|
| pHLS-EF1a-FRB-SpCas9-A | SpCas9, human codon optimized (Other) | | |
Hotta
| Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping.
Nat Commun. 2020 Mar 13;11(1):1334. doi: 10.1038/s41467-020-14957-y.
|
Expresses FRB fused SpCas9 for NanoMEDIC packaging.
pHLS
|
| eSpCas9(1.1) | eSpCas9(1.1) (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD
pFUGW
|
| SpCas9-HF1 | SpCas9-HF1 (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD
pFUGW
|
| HypaCas9 | HypaCas9 (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD
pFUGW
|
| evoCas9 | evoCas9 (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
pFUGW
|
| Sniper-Cas9 | Sniper-Cas9 (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD
pFUGW
|
| VQR variant | VQR (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
pFUGW
|
| VRER variant | VRER (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD
pFUGW
|
| VRQR variant | VRQR (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD
pFUGW
|
| VRQR-HF1 variant | VRQR-HF1 (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
pFUGW
|
| QQR1 variant | QQR1 (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
pFUGW
|
| xCas9 | xCas9 (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
pFUGW
|
| SpCas9-NG | SpCas9-NG (Synthetic) | EFS | Blasticidin |
Kim
| Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants
Nat Biotechnol (2020).
|
Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
pFUGW
|
| pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE | SpCas9 (Other) | | Puromycin |
Sanjana
| High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
| pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE | SpCas9-NG (Other) | | Puromycin |
Sanjana
| High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
| pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE | xCas9 3.7 (Other) | | Puromycin |
Sanjana
| High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
| pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE | xCas9-NG (Other) | | Puromycin |
Sanjana
| High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation.
Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010.
|
Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
lentiCRISPRv2
|
| pORANGE LOX Cloning template vector | SpCas9 (Other) | U6 | |
MacGillavry
| ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons.
PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665.
|
Cloning template for ORANGE-CAKE conditional knock-in constructs
pORANGE
|
| pCMV-T7-SpCas9-P2A-EGFP (RTW3027) | human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
JDS246
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpG-P2A-EGFP (RTW4177) | human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpRY-P2A-EGFP (RTW4830) | human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520) | human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160) | human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161) | human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576) | human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677) | human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505) | human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000) | human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008) | human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
RTW3027
Tag
/ Fusion Protein- BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
|
| pLentiCrispr-V2-mOrange (V2mO) | | pELF1a | Puromycin ; mOrange flurescent marker |
Ajani
| An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue.
PLoS One. 2020 Feb 13;15(2):e0228910. doi: 10.1371/journal.pone.0228910. eCollection 2020.
|
Lenti Crispr/Cas9 with mOrange fluorescent marker
pLentiCrispr-V2-mOrange (V2mO)
|
| Lenti-eCas9 | enhanced specificity Cas9 (1.1) (Synthetic) | EF-1a core | Puromycin |
Greber
| The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex.
Cell Rep. 2019 Dec 17;29(12):3785-3795.e8. doi: 10.1016/j.celrep.2019.11.064.
|
Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold
lentiCRISPR v2
Tag
/ Fusion Protein- 3xFLAG (N terminal on insert)
|
| pX165-Sniper-Cas9 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses Sniper Cas9 in mammalian cells
pX165
|
| pX165-LZ3 Cas9 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses LZ3 Cas9 in mammalian cells
pX165
|
| pX165-HiFi Cas9 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses HiFi Cas9 in mammalian cells
pX165
|
| pX165-eSpCas9 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses eSpCas9 in mammalian cells
pX165
|
| pX165-Cas9-HF1 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses Cas9-HF1 in mammalian cells
pX165
|
| pX165-HypaCas9 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses HypaCas9 in mammalian cells
pX165
|
| pX165-xCas9 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses xCas9 in mammalian cells
pX165
|
| pX165-evoCas9 | Cas9 (Other) | | |
Zhang
| Highly Parallel Profiling of Cas9 Variant Specificity.
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023.
|
Expresses evoCas9 in mammalian cells
pX165
|
| pDEST-Cas9-tdTomato | Cas9 (Other) | | |
Okano
| A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology.
PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019.
|
Mutisite Gateway mediated vector construction
pUC
|
| XClone_Cas9_KRAB_T2A_BFP | dCas9 (Other) | | Blasticidin |
Johnson
|
Tunable and temporal expression control of Cas9
(unpublished)
|
Tunable and temporal expression control of dCas9
XLone-GFP
|
| XClone-Cas9-Cronus-T2A-BFP | Cas9 (Other) | | Blasticidin |
Johnson
|
Tunable and temporal expression control of Cas9
(unpublished)
|
Tunable and temporal expression control of Cas9
XLone-GFP
|
| pCbh-SpyCas9-2A-puro-pA_U6-BbsI | SpyCas9 (Other) | Cbh | |
Kuehn
| A Homology Independent Sequence Replacement Strategy in Human Cells using a CRISPR nuclease
Open Biology
|
Mammalian expression vector for SpyCas9 and puromycin resistance
puc19
|
| px551-mSncg-0.27kb-HA-SpCas9 | Cas9 | Truncated mSncg (0.27kb) | |
Hu
| Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells.
J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 10.1523/JNEUROSCI.0102-20.2020.
|
Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells
pX551
|
| pQCi | | | GFP |
McComb
|
Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of large genetic payloads for rapid cell engineering
(unpublished)
|
Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)
pQCi
|
| pJ607-CMV-GFP-2A-Cas9 | GFP-2A-Cas9, HygR | CMV, SV40 promoter | Hygromycin |
Grav
| Multi-copy targeted integration for accelerated development of high-producing CHO cells.
ACS Synth Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322.
|
GFP-2A-Cas9 (CHO codon-optimized)
pJ607
|
| Sc++ | Sc++ (Other) | EF1ɑ | |
Jacobson
| An engineered ScCas9 with broad PAM range and high specificity and activity.
Nat Biotechnol. 2020 May 11. pii: 10.1038/s41587-020-0517-0. doi: 10.1038/s41587-020-0517-0.
|
Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells
Adapted from pCMV_AncBE4max (#112094)
|
| iSpyMac | iSpyMac (Other) | EF1ɑ | |
Jacobson
| A Cas9 with PAM recognition for adenine dinucleotides.
Nat Commun. 2020 May 18;11(1):2474. doi: 10.1038/s41467-020-16117-8.
|
Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells
Adapted from pCMV_AncBE4max (#112094)
|
| Cas9 CFP | spCas9 | | |
Carroll
|
Lenitviral sgRNA and Cas9 constructs
(unpublished)
|
Lentiviral Cas9 expression with CFP marker
Addgene #108100
|
| Cas9 NGFR | spCas9 | | |
Carroll
|
Lenitviral sgRNA and Cas9 constructs
(unpublished)
|
Lentiviral Cas9 expression with NGFR marker
Addgene #108100
|
| Lenti Cas9/SB100 | SB100X-P2A-spCas9 | | |
Carroll
|
Lenitviral sgRNA and Cas9 constructs
(unpublished)
|
Lentiviral expression of Cas9 and SB100
LentiV
|
| pRDA_085 | SpCas9 (Synthetic) | | |
Root
|
Massively parallel assessment of human variants with base editor screens
(unpublished)
|
Lentiviral expression of SpCas9; P2A site provides mKate2
pXPR
|
| pX330-gRNA | gRNA3 (Synthetic) | U6 | |
Lai
| A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo.
Nucleic Acids Res. 2020 Aug 14. pii: 5892751. doi: 10.1093/nar/gkaa669.
|
A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.
pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pMSCVpuro-Cas9-EGFP | Cas9 fused to EGFP (Homo sapiens) | MSCV LTR promoter | Puromycin |
Ha
| Very fast CRISPR on demand.
Science. 2020 Jun 12;368(6496):1265-1269. doi: 10.1126/science.aay8204.
|
Expresses active Cas9 fused with EGFP for fluorescence microscopy
pMSCVpuro
|
| pAAV-pMecp2-SaCas9-pU6-sgRNA | SaCas9 (Synthetic) | pMecp2 | |
Tin
| Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa.
Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23.
|
Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons
pX601 (Addgene #61591)
|
| CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector | AsCpf1-HA-2A-GFP (Other) | U6 | |
Jaenisch
| Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction.
Cell Reports: Medicine
|
A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)
pCDNA3.1
|
| pEJS1089: mini-AAV.sgRNA.Nme2Cas9 | Nme2Cas9 with single guide RNA cassette | U1a | |
Sontheimer
| Precision Cas9 Genome Editing in vivo with All-in-one, Self-targeting AAV Vectors
bioRxiv October 9, 2020
|
Delivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 Kb
Nme2Cas9_AAV (Plasmid #119924)
|
| pEJS1099: Dual-sgRNA.Design 4 | Nme2Cas9 with two guide RNA cassettes | U1a | |
Sontheimer
| Precision Cas9 Genome Editing in vivo with All-in-one, Self-targeting AAV Vectors
bioRxiv October 9, 2020
|
Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector
p1089: minimized AAV.sgRNA.Nme2Cas9
|
| pX330 NL-FKBP-Cas9 | FKBP F36V (Homo sapiens) | CBh | |
Choudhary
| Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes
ACS Cent. Sci. 2020. November 18, 2020
|
Mammalian expression
pUC ori vector, pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pX330 LC-FKBP-Cas9 | FKBP F36V (Homo sapiens) | CBh | |
Choudhary
| Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes
ACS Cent. Sci. 2020. November 18, 2020
|
Mammalian expression
pUC ori vector, pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pX330 NC-FKBP-Cas9 | FKBP F36V (Homo sapiens) | CBh | |
Choudhary
| Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes
ACS Cent. Sci. 2020. November 18, 2020
|
Mammalian expression
pUC ori vector, pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pX330 N-FKBP-Cas9 | FKBP F36V (Homo sapiens) | CBh | |
Choudhary
| Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes
ACS Cent. Sci. 2020. November 18, 2020
|
Mammalian expression
pUC ori vector, pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pX330 L-FKBP-Cas9 | FKBP F36V (Homo sapiens) | CBh | |
Choudhary
| Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes
ACS Cent. Sci. 2020. November 18, 2020
|
Mammalian expression
pUC ori vector, pX330-U6-Chimeric_BB-CBh-hSpCas9
|
| pLentiCRISPR-v1-sgGPT2_5 | sgRNA 5 targeting GPT2 (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting GPT2
pLentiCRISPR v1
GPT2 ALT2, GPT 2, MRT49
|
| pLentiCRISPR-v1-sgGPT2_9 | sgRNA 9 targeting GPT2 (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting GPT2
pLentiCRISPR v1
GPT2 ALT2, GPT 2, MRT49
|
| pLentiCRISPR-v1-sgMPC2_7 | sgRNA 7 targeting GPT2 (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting MPC2
pLentiCRISPR v1
MPC2 BRP44
|
| pLentiCRISPR-v1-sgMPC2_9 | sgRNA 9 targeting GPT2 (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting MPC2
pLentiCRISPR v1
MPC2 BRP44
|
| pLentiCRISPR-v1-sgGLS_2 | sgRNA 2 targeting GLS (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting GLS
pLentiCRISPR v1
GLS AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, KGA
|
| pLentiCRISPR-v1-sgGLS_6 | sgRNA 6 targeting GLS (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting GLS
pLentiCRISPR v1
GLS AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG, GLS1, KGA
|
| pLentiCRISPR-v1-sgHK2_2 | sgRNA 2 targeting HK2 (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting HK2
pLentiCRISPR v1
HK2 HKII, HXK2
|
| pLentiCRISPR-v1-sgNADK_1 | sgRNA 1 targeting NADK (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting NADK
pLentiCRISPR v1
NADK RP1-283E3.6, dJ283E3.1
|
| pLentiCRISPR-v1-sgMETAP1_2 | sgRNA 2 targeting METAP1 (Homo sapiens) | U6 | Puromycin |
Cantor
| CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.
Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 10.1016/j.cmet.2021.02.005.
|
lentiviral vector expressing Cas9 and an sgRNA targeting METAP1
pLentiCRISPR v1
METAP1 MAP1A, MetAP1A
|
| pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9 | mNeonGreen-P2A-Cas9 (Synthetic) | EFS | mNeonGreen |
Liu
| DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cells
bioRxiv (2019)
|
Makes a lentivirus that expresses mNeonGreen and Cas9.
lenti, 3rd generation
|
| pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP | SpCas9 (Synthetic) | CMV | Hygromycin |
Kim
| Recording of elapsed time and temporal information about biological events using Cas9.
Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 10.1016/j.cell.2021.01.014.
|
Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)
pcDNA5-FRT
cas9 B6D67_RS04280, ERS445054_00848
|
| pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP | ciCas9 (Synthetic) | CMV | Hygromycin |
Kim
| Recording of elapsed time and temporal information about biological events using Cas9.
Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 10.1016/j.cell.2021.01.014.
|
Expresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)
pcDNA5-FRT
|
| Lenti_CMV-Cas9-P2A-HygR | SpCas9 (Synthetic) | CMV | Hygromycin |
Kim
| Recording of elapsed time and temporal information about biological events using Cas9.
Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 10.1016/j.cell.2021.01.014.
|
Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells
lentiCas9-Blast plasmid (Addgene #52962)
cas9 B6D67_RS04280, ERS445054_00848
|
| Lenti_PGK-Cas9-P2A-HygR | SpCas9 (Synthetic) | PGK | Hygromycin |
Kim
| Recording of elapsed time and temporal information about biological events using Cas9.
Cell. 2021 Jan 28. pii: S0092-8674(21)00014-3. doi: 10.1016/j.cell.2021.01.014.
|
Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells
lentiCas9-Blast plasmid (Addgene #52962)
cas9 B6D67_RS04280, ERS445054_00848
|
| pREDIT_Cas9-MS2-BB_BbsI | | | |
Cong
|
Microbial Single-strand Annealing Proteins Enable CRISPR Gene-editing Tools with Improved Knock-in Efficiencies and Reduced Off-target Effects
(unpublished)
|
REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP
pX330
|
| pCBH-Cas9-Suntag-BB_BbsI | | | |
Cong
|
Microbial Single-strand Annealing Proteins Enable CRISPR Gene-editing Tools with Improved Knock-in Efficiencies and Reduced Off-target Effects
(unpublished)
|
REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP
pX330
|