| pK7WGF2::hCas9 | hCas9 (Synthetic) | Kanamycin |
Kamoun
| Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.
Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.
|
Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
pK7WGF2
|
| pICH41308::hCas9 | hCas9 (Synthetic) | |
Kamoun
| Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system.
Plant Methods. 2013 Oct 11;9(1):39.
|
Level 0 hCas9 module
pICH41308
|
| pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST | 35Sp::hCas9 (Synthetic) | |
Kamoun
| Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system.
Plant Methods. 2013 Oct 11;9(1):39.
|
Level 1 hCas9 module
pICH47742
|
| pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1 | sgRNA_PDS2-Cas9-sgRNA_PDS1 (Synthetic) | |
Kamoun
| Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system.
Plant Methods. 2013 Oct 11;9(1):39.
|
Level 2 construct
pAGM4723
|
| pBUN411 | 3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic) | Bar |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
pGreen-like binary vector
|
| pHSN401 | 3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic) | Hygromycin |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
pGreen-like binary vector
|
| pRGE31 | | |
Yang
| RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17.
|
Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter
pUGW11
|
| pRGEB31 | | Hygromycin |
Yang
| RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17.
|
Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation.
pCAMBIA1300
|
| HBT-pcoCas9 | pcoCas9 (Synthetic) | |
Sheen
| Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9.
Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
|
transient expression of pcoCas9 gene in plant cells
HBT-FLAG
|
| pFGC-pcoCas9 | 35SPPDK:pcoCas9:NOS cassette (Synthetic) | Basta |
Sheen
|
Sheen lab CRISPR plasmids
(unpublished)
|
A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
pFGC-RCS
|
| pJIT163-2NLSCas9 | Cas9 (Other) | |
Gao
| Targeted genome modification of crop plants using a CRISPR-Cas system.
Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
|
Expression of rice codon-optimized Cas9 in plant cells
pJIT163
|
| p201N Cas9 | Cas9 (Synthetic), nptII (Other) | G418, kanamycin |
Parrott
| Targeted genome modifications in soybean with CRISPR/Cas9.
BMC Biotechnology. 2015;15:16
|
Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
pPZP
|
| p201H Cas9 | Cas9 (Synthetic), hph (Other) | Hygromycin |
Parrott
| Targeted genome modifications in soybean with CRISPR/Cas9.
BMC Biotechnology. 2015;15:16
|
Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
pPZP
|
| p201B Cas9 | Cas9 (Synthetic), BAR (Other) | Basta |
Parrott
| Targeted genome modifications in soybean with CRISPR/Cas9.
BMC Biotechnology. 2015;15:16
|
Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
pPZP
|
| p201G Cas9 | Cas9 (Synthetic), sGFP (Synthetic) | GFP |
Parrott
| Targeted genome modifications in soybean with CRISPR/Cas9.
BMC Biotechnology. 2015;15:16
|
Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
pPZP
|
| Cas9 MDC123 | Glycine Max codon optimized Cas9 (Other) | Basta |
Stupar
| CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme.
GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
|
2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection
pMDC123
|
| Cas9 MDC32 | Glycine Max codon optimized Cas9 (Other) | Hygromycin |
Stupar
| CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme.
GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
|
2x35S promoting Glycing Max codon optimized Cas9 with hygromycin plant selection
pMDC32
|
| G10 Cas9 MDC123 | Glycine Max codon optimized Cas9 (Other) | Basta |
Stupar
| CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme.
GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
|
G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection
pMDC123
|
| pDe-CAS9 | Cas9 (Arabidopsis thaliana) | Basta |
Puchta
| Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.
|
Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence
pPZP221
|
| pCAS9-TPC | Cas9 (Arabidopsis thaliana) | Basta |
Puchta
| Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.
|
Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning
pPZP221
|
| pBUE411 | gRNA scaffold (Synthetic), zCas9 (Other) | Basta |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
pCambia
|
| pHSE401 | gRNA scaffold (Synthetic), zCas9 (Other) | Hygromycin |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
pCambia
|
| pKSE401 | gRNA scaffold (Synthetic), zCas9 (Other) | Neomycin (select with G418) |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
pCambia
|
| pHUE411 | gRNA scaffold (Synthetic), zCas9 (Other) | Hygromycin |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance
pCambia
|
| pBUN421 | gRNA scaffold (Synthetic), zCas9 (Other) | Basta |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
pGreen-like
|
| pRGEB32 | | Hygromycin |
Yang
| Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2.
|
Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
pRGEB31
|
| pRGE32 | | |
Yang
| Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2.
|
Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast
pRGE31
|
| pYLCRISPR/Cas9Pubi-H | Cas9 (Other) | Hygromycin |
Liu
| A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
|
expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection
pCAMBIA1300
|
| pYLCRISPR/Cas9Pubi-B | Cas9 (Other) | Basta |
Liu
| A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
|
expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection
pCAMBIA1300
|
| pYLCRISPR/Cas9P35S-H | Cas9 (Other) | Hygromycin |
Liu
| A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
|
expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection
pCAMBIA1300
|
| pYLCRISPR/Cas9P35S-B | Cas9 (Other) | Basta |
Liu
| A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
|
expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
pCAMBIA1300
|
| pYLCRISPR/Cas9P35S-N | Cas9 (Other) | Neomycin (select with G418) |
Liu
| A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
|
expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
pCAMBIA1300
|
| pEGB2alpha2 35s:hCas9:tNos (GB0639) | hCas9 (Other) | |
Orzaez
| GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.
Plant Physiol. 2013 Jul;162(3):1618-31.
|
Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter
pDGB2alpha2
|
| pICSL11056 | Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s (Other) | |
Patron
| Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease.
Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
|
Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants
pICH47742 (AddGene #47742)
|
| pICSL11060 | Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s (Other) | |
Patron
| Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease.
Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
|
Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)
pICH47742 (AddGene #47742)
|
| pYPQ150 | pcoCas9 (plant codon-optimized) (Other) | |
Qi
| A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation.
Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
|
Gateway entry vector with pcoCas9 (Plant codon optimized)
pNJB91
|
| pYPQ154 | AteCas9 (Arabidopsis codon optimized) (Other) | |
Qi
| A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation.
Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
|
Gateway entry vector with AteCas9 (Arabidopsis codon optimized)
pYPQ185-linker2
|
| pYPQ158 | hSpCas9 (Human codon optimized) (Other) | |
Qi
| A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation.
Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
|
Gateway entry vector with hSpCas9 (Human codon optimized)
pNJB185-linker6
|
| pYPQ167 | Cas9p (Plant codon-optimized; high GC content at 5 prime region) (Other) | |
Qi
| A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation.
Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
|
Gateway entry vector with Cas9p
pYPQ185-linker2
|
| pTC217 | Nuclease (Cas9/sgRNA) + Donor + GVR (Other) | kanamycin |
Voytas
| High-frequency, precise modification of the tomato genome.
Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
|
Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
pLSLR
|
| pTC223 | Nuclease (Cas9/sgRNA) + Donor + GVR (Other) | kanamycin |
Voytas
| High-frequency, precise modification of the tomato genome.
Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
|
Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
pLSLR
|
| pHEE401 | sgRNA scaffold (Synthetic), zCas9 (Other) | Hygromycin |
Chen
| Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
|
Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
pCambia
|
| pHEE401E | gRNA scaffold (Synthetic), zCas9 (Other) | Hygromycin |
Chen
| Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
|
Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
pCambia
|
| pHEE2E-TRI | sgRNA targeting TRY and CPC genes (Synthetic), sgRNA targeting ETC2 gene (Synthetic), zCas9 (Other) | Hygromycin |
Chen
| Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
|
Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
pCambia
|
| pMpGE010 | | Hygromycin |
Kohchi
| Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.
PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.
|
For CRISPR/Cas9 genome editing in Marchantia polymorpha
pMpGWB103
|
| pMpGE011 | | Chlorsulfuron |
Kohchi
| Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.
PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.
|
For CRISPR/Cas9 genome editing in Marchantia polymorpha
pMpGWB303
|
| pEGB2Ω2_SF-35s:hCas9:tNos (GB1103) | hCas9 (Other) | |
Orzaez
| A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.
Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.
|
Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.
pDGB2omega2
|
| pBAtC | | Basta |
Kim
| A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system.
J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
|
Plant expression of Cas9 and gRNA empty backbone; Basta resistance
pB2GW7
Tag
/ Fusion Protein- NLS-HA (C terminal on insert)
|
| pHAtC | | Hygromycin |
Kim
| A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system.
J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
|
Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance
pH2GW7
|
| pHDE-35S-Cas9-mCherry | | Hygromycin ; mCherry fluorescence |
Zhao
| An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing.
Plant Physiol. 2016 May 15. pii: pp.00663.2016.
|
For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants
pHDE
|
| pHDE-35S-Cas9-mCherry-UBQ | | Hygromycin ; mCherry fluorescence |
Zhao
| An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing.
Plant Physiol. 2016 May 15. pii: pp.00663.2016.
|
For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs
pHDE
|
| pKIR1.1 | human-codon-optimized SpCas9 (Other) | Hygromycin ; TagRFP in seeds |
Higashiyama
| pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana.
Plant Cell Physiol. 2016 Nov 17. pii: pcw191.
|
CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter
pFAST-R01
|
| pKI1.1 | human-codon-optimized SpCas9 (Other) | |
Higashiyama
| pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana.
Plant Cell Physiol. 2016 Nov 17. pii: pcw191.
|
CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO
pENTR/D-TOPO
|
| pKI1.1R | human-codon-optimized SpCas9 (Other) | Hygromycin ; TagRFP in seeds |
Higashiyama
| pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana.
Plant Cell Physiol. 2016 Nov 17. pii: pcw191.
|
CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.
pFAST-R01
|
| pAGM4723:TpCC_Urease | Cas9 (Other), Urease-targeting gRNA 1 (Other), Urease-targeting gRNA 2 (Other) | Nourseothricin (Nat) |
Mock
| Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana
Plant Methods 2016, 12:49
|
Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs
pAGM4723
|
| pICH47742:FCP:Cas9YFP | Cas9 (Other) | |
Mock
| Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana
Plant Methods 2016, 12:49
|
Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter
pICH47742
|
| pYPQ220 (AsCpf1) | AsCpf1 (Other) | |
Qi
| A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants.
Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.
|
AsCpf1 Gateway entry plasmid
pYPQ167
|
| pYPQ230 (LbCpf1) | LbCpf1 (Other) | |
Qi
| A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants.
Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.
|
LbCpf1 Gateway entry plasmid
pYPQ167
|
| pKIR1.0 | human-codon-optimized SpCas9 (Other) | TagRFP in seeds |
Higashiyama
| pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana.
Plant Cell Physiol. 2016 Nov 17. pii: pcw191.
|
CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.
pFAST-R01
|
| pUB-Cas9 | Cas9 (Synthetic) | Hygromycin |
Weber
| An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana.
Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017.
|
For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
pKB65
|
| [email protected] | sgRNA against GLABROUS1 (Synthetic) | Hygromycin |
Weber
| An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana.
Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017.
|
Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9
pUB-Cas9
|
| pTX168 | | Hygromycin |
Voytas
| A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants.
Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.
|
Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants
pCAMBIA1300
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pTX171 | | Hygromycin |
Voytas
| A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants.
Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.
|
Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants
pTX168
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pTX172 | | Hygromycin |
Voytas
| A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants.
Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.
|
Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants
pTX171
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pTX176 | | Hygromycin |
Voytas
| A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants.
Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.
|
Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants
pTX171
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pJG80 | TaCas9 (Other) | |
Voytas
| High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9.
Plant J. 2016 Dec 10. doi: 10.1111/tpj.13446.
|
Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites
unknown
|
| pMOD_A0108 | AtCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A0502 | Csy4-P2A-AtCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A0508 | Csy4-P2A-AtCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A0901 | TREX2-P2A-AtCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A0908 | TREX2-P2A-AtCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A1110 | TaCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A1111 | TaCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A1510 | Csy4-P2A-TaCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP
pMOD_A1001
|
| pMOD_A1511 | Csy4-P2A-TaCas9 (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP
pMOD_A1001
|
| pDIRECT_21A | Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic) | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II
pTRANS_210
|
| pDIRECT_21C | Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic) | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
pTRANS_210d
|
| pDIRECT_22A | Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic) | Kanamycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II
pTRANS_220
|
| pDIRECT_22C | Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic) | Kanamycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
pTRANS_220d
|
| pDIRECT_23A | Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic) | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar
pTRANS_230
|
| pDIRECT_23C | Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic) | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar
pTRANS_230d
|
| pDIRECT_25F | Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA (Synthetic) | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II
pTRANS_250d
|
| pDIRECT_25H | Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers (Synthetic) | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
pTRANS_250d
|
| pDIRECT_26H | Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers (Synthetic) | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
pTRANS_260d
|
| pAtCas9_1 | AtCas9 (Synthetic) | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II
pCAMBIA
|
| pAtCas9_3 | AtCas9 (Synthetic) | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar
pCAMBIA
|
| pTaCas9_5 | TaCas9 (Synthetic) | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II
pCAMBIA
|
| pTaCas9_6 | TaCas9 (Synthetic) | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar
pCAMBIA
|
| pDIRECT_10A | 35S:AtCas9 + AtU6:gRNA (Synthetic) | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none
pTRANS_100
|
| pKEE401 | | Neomycin (select with G418) |
Chen
| Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
|
CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance
pCambia
|
| pXEE401B | | |
Chen
| Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
|
CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance
pCambia
|
| pXEE401BG | | |
Chen
| Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
|
CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance
pCambia
|
| pSC10 | | |
Stupar
| CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula.
Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.
|
pAH595-gRNA 2xplex entry cassette
pENTR top
|
| pSC12 | | |
Stupar
| CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula.
Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.
|
pNB184-Cas9 entry cassette
pENTR top
|
| pYLCRISPR/Cas9pUbi-N | Cas9 (Synthetic) | |
Liu
| A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants.
Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.
|
Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection
pCAMBIA1300
|
| pJKW0458 | | |
Weng
|
pJKW0457
(unpublished)
|
p35S:CAS9
pEarleyGate100
|
| pJKW0474 | | |
Weng
|
pJKW0457
(unpublished)
|
pCLV3:Cas9
pEarleyGate100
|
| pMpGE006 | Atco-Cas9-Pea3ter (Synthetic) | Hygromycin |
Sugano
| Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.
PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.
|
Cas9 expression in M.polymorpha
pMpGWB103
|
| pYPQ239 | FnCpf1 (Other) | |
Qi
| Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites.
Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20.
|
FnCpf1 Gateway entry plasmid
pYPQ167
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| pORFE0001 | AtCAS9 (Other) | |
Chevalier
|
Chevalier Lab plasmids
(unpublished)
|
AtCas9 module CDS1
pICH41308
|
| pORFE1001 | AtCAS9 (Other) | |
Chevalier
|
Chevalier Lab plasmids
(unpublished)
|
Overexpression of the AtCAS9 in plant under 2x35S promoter
pICH47742
|
| pMH-Cas9-gate | Cas9 (Other) | Hygromycin |
Bezanilla
| Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens.
Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.
|
Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.
pGEM-T easy
|
| pMK-Cas9-gate | Cas9 (Other) | Neomycin (select with G418) |
Bezanilla
| Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens.
Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.
|
Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.
pGEM-T easy
|
| pZeo-Cas9-gate | Cas9 (Other) | Zeocin |
Bezanilla
| Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens.
Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.
|
Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.
pGEM-T easy
|
| pICSL90016 | BsaI:AATG:Cas9_2:GCTT:BsaI (Synthetic) | |
Jones
| Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis
bioRxiv 419952
|
Cas9_2 Golden Gate Level 0
pICH41308
|
| BCJJ180 | BsaI:AATG:Cas9_4:GCTT:BsaI (Synthetic) | |
Jones
| Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis
bioRxiv 419952
|
Cas9_4 Golden Gate Level 0
pICH41308
|
| pICSL90005 | SpCas9-h (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)
pAGM1287
|
| pEPOR0SP0013 | SpCas9-p (with stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SpCas9-p
pICH41308
|
| pEPOR0SP0009 | SpCas9-p (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)
pAGM1287
|
| pEPOR0SP0001 | SpCas9-DE (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)
pAGM1287
|
| pEPOR0SP0014 | SpCas9-KA (with stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SpCas9-KA
pICH41308
|
| pEPOR0SP0015 | SpCas9-KA (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)
pAGM1287
|
| pEPOR0SP0016 | Sp-eCas9 1.0 (with stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, Sp-eCas9 1.0
pICH41308
|
| pEPOR0SP0017 | Sp-eCas9 1.0 (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)
pAGM1287
|
| pEPOR0SP0018 | Sp-eCas9 1.1 (with stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, Sp-eCas9 1.1
pICH41308
|
| pEPOR0SP0019 | Sp-eCas9 1.1 (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)
pAGM1287
|
| pEPOR0SP0011 | Sp-xCas9 3.7 (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)
pAGM1287
|
| pEPOR0SP0020 | SaCas9 (with stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SaCas9
pICH41308
|
| pEPOR0SP0021 | SaCas9 (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, SaCas9 (no stop codon)
pAGM1287
|
| pEPOR0SP0012 | eSaCas9 (no stop codon) (Other) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)
pAGM1287
|
| pICSL11023 | 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
pICH47742
|
| pEPOR1CB0002 | 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
pICH47742
|
| pEPOR1CB0009 | 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plants
pICH47742
|
| pEPOR1CB0011 | 2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421) (Synthetic) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plants
pICH47742
|
| pEPOR1CB0112 | 2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421) (Synthetic) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants
pICH47742
|
| pEPOR1CB0015 | 2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421) (Synthetic) | |
Patron
| Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering.
bioRxiv 422766
|
Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants
pICH47742
|
| p221z-CAS9p-t35s | CAS9p (Other) | |
Mähönen
|
Plants CRISPR plasmids
(unpublished)
|
Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system
pDONR-221z
|
| p221z-CAS9p-TagRFP-t35s | CAS9p-TagRFP (Other) | |
Mähönen
|
Plants CRISPR plasmids
(unpublished)
|
Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system
pDONR-221z
|
| pYPQ150-x3.7 | pcoCas9-x3.7 | |
Qi
| Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG.
Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.
|
Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized)
pNJB91
|
| pGEL062 | | Hygromycin |
Zhang
|
Yong Zhang lab CRISPR plasmids
(unpublished)
|
For genome editing in plant by SpCas9 wild type
pZHY988
|
| pFH13 | SpCas9, wheat codon optimized | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 SpCas9, wheat codon optimized
pICH41308
|
| pFH14 | SaCas9, Arabidopsis codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 SaCas9, Arabidopsis codon optimized
pICH41308
|
| pFH15 | SaCas9, Arabidopsis codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 StCas9, Arabidopsis codon optimized
pICH41308
|
| pFH16 | FnCas12a, rice codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 FnCas12a, rice codon optimized
pICH41308
|
| pFH17 | LbCas12a, rice codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 LbCas12a, rice codon optimized
pICH41308
|
| pFH22 | xCas9 3.7 (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 xCas9 3.7
pICH41308
|
| pFH32 | SpCas9-NG, human codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 SpCas9-NG, human codon optimized
pICH41308
|
| pFH46 | FnCas12a, human codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 FnCas12a, human codon optimized
pICH41308
|
| pFH47 | LbCas12a, human codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 LbCas12a, human codon optimized
pICH41308
|
| pFH48 | Csy4 (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 Csy4
pICH41308
|
| pFH76 | ScCas9, wheat codon optimized (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 ScCas9, wheat codon optimized
pICH41308
|
| pRGEB32-BAR | | Basta ; BAR |
Hunter
|
CRISPR/Cas9 vector optimized for Agrobacterium-mediated transformation of maize (with BAR selectable marker) (modified from pRGEB32)
(unpublished)
|
For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.
pRGEB32
|
| pENTR_Wheat_live_Cas9 | Wheat_live_Cas9 (Synthetic) | |
Kamoun
|
Genome editing in wheat
(unpublished)
|
Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.
pENTR4
|
| pICSL11085_GG_Wheat_live_Cas9_for | Wheat_live_Cas9 (Synthetic) | |
Kamoun
|
Genome editing in wheat
(unpublished)
|
Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
pICH47742
|
| pICSL11086_GG_Wheat_live_Cas9_rev | Wheat_live_Cas9 (Synthetic) | |
Kamoun
|
Genome editing in wheat
(unpublished)
|
Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
pICH47742
|
| pFH23 | ZmUBIp::SpCas9 (wheat codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt
pICH47742
|
| pFH52 | 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
pICH47742
|
| pFH53 | 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
pICH47742
|
| pFH54 | AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
pICH47742
|
| pFH58 | ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
pICH47742
|
| pFH59 | ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt
pICH47742
|
| pFH60 | ZmUBIp::FnCas12a (rice codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::FnCas12a (rice codon optimized):NOSt
pICH47742
|
| pFH61 | ZmUBIp::LbCas12a (rice codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt
pICH47742
|
| pFH68 | ZmUBIp::SpCas9-NG (human codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt
pICH47742
|
| pFH80 | ZmUBIp::xCas9 3.7:NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::xCas9 3.7:NOSt
pICH47742
|
| pFH81 | ZmUBIp::ScCas9 (wheat codon optimized):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt
pICH47742
|
| pFH24 | SpCas9, wheat codon optimized, version 2 (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 SpCas9, wheat codon optimized (version 2)
pICH41308
|
| pFH25 | SpCas9, wheat codon optimized, version 3 (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level0 SpCas9, wheat codon optimized (version 3)
pICH41308
|
| pFH66 | ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
pICH47742
|
| pFH67 | ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt (Synthetic) | |
Nekrasov
| A modular cloning toolkit for genome editing in plants
bioRxiv
|
Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt
pICH47742
|
| pG3H-U3Ub | CRISPR/Cas9 (Synthetic) | |
Chen
| A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize.
Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.
|
pGreen3 CRISPR/Cas9
pGreen3
|
CRISPR Plasmids: Plants