CRISPR Plasmids: Plants

The following CRISPR plasmids have been designed for use in plants.

Cut

Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.

To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ.

	Plasmid	Gene/Insert	Selectable Marker	PI	Publication	Hidden Extra Search Info
	pK7WGF2::hCas9	hCas9 (Synthetic)	Kanamycin	Kamoun	Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.	Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue pK7WGF2
	pICH41308::hCas9	hCas9 (Synthetic)		Kamoun	Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39.	Level 0 hCas9 module pICH41308
	pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST	35Sp::hCas9 (Synthetic)		Kamoun	Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39.	Level 1 hCas9 module pICH47742
	pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1	sgRNA_PDS2-Cas9-sgRNA_PDS1 (Synthetic)		Kamoun	Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39.	Level 2 construct pAGM4723
	pBUN411	3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic)	Bar	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
	pHSN401	3×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic)	Hygromycin	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
	pRGE31			Yang	RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17.	Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by rice snoRNA U3 promoter pUGW11
	pRGEB31		Hygromycin	Yang	RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17.	Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plant. by agrobacterium mediated transformation. pCAMBIA1300
	HBT-pcoCas9	pcoCas9 (Synthetic)		Sheen	Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.	transient expression of pcoCas9 gene in plant cells HBT-FLAG
	pFGC-pcoCas9	35SPPDK:pcoCas9:NOS cassette (Synthetic)	Basta	Sheen	Sheen lab CRISPR plasmids (unpublished)	A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA pFGC-RCS
	pJIT163-2NLSCas9	Cas9 (Other)		Gao	Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.	Expression of rice codon-optimized Cas9 in plant cells pJIT163
	p201N Cas9	Cas9 (Synthetic), nptII (Other)	G418, kanamycin	Parrott	Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16	Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
	p201H Cas9	Cas9 (Synthetic), hph (Other)	Hygromycin	Parrott	Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16	Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
	p201B Cas9	Cas9 (Synthetic), BAR (Other)	Basta	Parrott	Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16	Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
	p201G Cas9	Cas9 (Synthetic), sGFP (Synthetic)	GFP	Parrott	Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnology. 2015;15:16	Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle pPZP
	Cas9 MDC123	Glycine Max codon optimized Cas9 (Other)	Basta	Stupar	CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.	2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection pMDC123
	Cas9 MDC32	Glycine Max codon optimized Cas9 (Other)	Hygromycin	Stupar	CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.	2x35S promoting Glycing Max codon optimized Cas9 with hygromycin plant selection pMDC32
	G10 Cas9 MDC123	Glycine Max codon optimized Cas9 (Other)	Basta	Stupar	CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.	G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection pMDC123
	pDe-CAS9	Cas9 (Arabidopsis thaliana)	Basta	Puchta	Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.	Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence pPZP221
	pCAS9-TPC	Cas9 (Arabidopsis thaliana)	Basta	Puchta	Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.	Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning pPZP221
	pBUE411	gRNA scaffold (Synthetic), zCas9 (Other)	Basta	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pCambia
	pHSE401	gRNA scaffold (Synthetic), zCas9 (Other)	Hygromycin	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pCambia
	pKSE401	gRNA scaffold (Synthetic), zCas9 (Other)	Neomycin (select with G418)	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance pCambia
	pHUE411	gRNA scaffold (Synthetic), zCas9 (Other)	Hygromycin	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance pCambia
	pBUN421	gRNA scaffold (Synthetic), zCas9 (Other)	Basta	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance pGreen-like
	pRGEB32		Hygromycin	Yang	Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2.	Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation. pRGEB31
	pRGE32			Yang	Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2.	Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast pRGE31
	pYLCRISPR/Cas9Pubi-H	Cas9 (Other)	Hygromycin	Liu	A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.	expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection pCAMBIA1300
	pYLCRISPR/Cas9Pubi-B	Cas9 (Other)	Basta	Liu	A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.	expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection pCAMBIA1300
	pYLCRISPR/Cas9P35S-H	Cas9 (Other)	Hygromycin	Liu	A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.	expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection pCAMBIA1300
	pYLCRISPR/Cas9P35S-B	Cas9 (Other)	Basta	Liu	A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.	expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection pCAMBIA1300
	pYLCRISPR/Cas9P35S-N	Cas9 (Other)	Neomycin (select with G418)	Liu	A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.	expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection pCAMBIA1300
	pEGB2alpha2 35s:hCas9:tNos (GB0639)	hCas9 (Other)		Orzaez	GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol. 2013 Jul;162(3):1618-31.	Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter pDGB2alpha2
	pICSL11056	Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s (Other)		Patron	Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.	Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants pICH47742 (AddGene #47742)
	pICSL11060	Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s (Other)		Patron	Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.	Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea) pICH47742 (AddGene #47742)
	pYPQ150	pcoCas9 (plant codon-optimized) (Other)		Qi	A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.	Gateway entry vector with pcoCas9 (Plant codon optimized) pNJB91
	pYPQ154	AteCas9 (Arabidopsis codon optimized) (Other)		Qi	A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.	Gateway entry vector with AteCas9 (Arabidopsis codon optimized) pYPQ185-linker2
	pYPQ158	hSpCas9 (Human codon optimized) (Other)		Qi	A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.	Gateway entry vector with hSpCas9 (Human codon optimized) pNJB185-linker6
	pYPQ167	Cas9p (Plant codon-optimized; high GC content at 5 prime region) (Other)		Qi	A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.	Gateway entry vector with Cas9p pYPQ185-linker2
	pTC217	Nuclease (Cas9/sgRNA) + Donor + GVR (Other)	kanamycin	Voytas	High-frequency, precise modification of the tomato genome. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.	Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b pLSLR
	pTC223	Nuclease (Cas9/sgRNA) + Donor + GVR (Other)	kanamycin	Voytas	High-frequency, precise modification of the tomato genome. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.	Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7 pLSLR
	pHEE401	sgRNA scaffold (Synthetic), zCas9 (Other)	Hygromycin	Chen	Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.	Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance pCambia
	pHEE401E	gRNA scaffold (Synthetic), zCas9 (Other)	Hygromycin	Chen	Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.	Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance pCambia
	pHEE2E-TRI	sgRNA targeting TRY and CPC genes (Synthetic), sgRNA targeting ETC2 gene (Synthetic), zCas9 (Other)	Hygromycin	Chen	Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.	Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC pCambia
	pMpGE010		Hygromycin	Kohchi	Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.	For CRISPR/Cas9 genome editing in Marchantia polymorpha pMpGWB103
	pMpGE011		Chlorsulfuron	Kohchi	Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.	For CRISPR/Cas9 genome editing in Marchantia polymorpha pMpGWB303
	pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)	hCas9 (Other)		Orzaez	A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.	Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181. pDGB2omega2
	pBAtC		Basta	Kim	A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.	Plant expression of Cas9 and gRNA empty backbone; Basta resistance pB2GW7 Tag / Fusion Protein NLS-HA (C terminal on insert)
	pHAtC		Hygromycin	Kim	A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system. J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.	Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance pH2GW7
	pHDE-35S-Cas9-mCherry		Hygromycin ; mCherry fluorescence	Zhao	An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing. Plant Physiol. 2016 May 15. pii: pp.00663.2016.	For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants pHDE
	pHDE-35S-Cas9-mCherry-UBQ		Hygromycin ; mCherry fluorescence	Zhao	An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing. Plant Physiol. 2016 May 15. pii: pp.00663.2016.	For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs pHDE
	pKIR1.1	human-codon-optimized SpCas9 (Other)	Hygromycin ; TagRFP in seeds	Higashiyama	pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191.	CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter pFAST-R01
	pKI1.1	human-codon-optimized SpCas9 (Other)		Higashiyama	pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191.	CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO pENTR/D-TOPO
	pKI1.1R	human-codon-optimized SpCas9 (Other)	Hygromycin ; TagRFP in seeds	Higashiyama	pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191.	CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter. pFAST-R01
	pAGM4723:TpCC_Urease	Cas9 (Other), Urease-targeting gRNA 1 (Other), Urease-targeting gRNA 2 (Other)	Nourseothricin (Nat)	Mock	Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana Plant Methods 2016, 12:49	Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs pAGM4723
	pICH47742:FCP:Cas9YFP	Cas9 (Other)		Mock	Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana Plant Methods 2016, 12:49	Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter pICH47742
	pYPQ220 (AsCpf1)	AsCpf1 (Other)		Qi	A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.	AsCpf1 Gateway entry plasmid pYPQ167
	pYPQ230 (LbCpf1)	LbCpf1 (Other)		Qi	A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.	LbCpf1 Gateway entry plasmid pYPQ167
	pKIR1.0	human-codon-optimized SpCas9 (Other)	TagRFP in seeds	Higashiyama	pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Plant Cell Physiol. 2016 Nov 17. pii: pcw191.	CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter. pFAST-R01
	pUB-Cas9	Cas9 (Synthetic)	Hygromycin	Weber	An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana. Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017.	For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017) pKB65
	[email protected]	sgRNA against GLABROUS1 (Synthetic)	Hygromycin	Weber	An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana. Front Plant Sci. 2017 Jan 24;8:39. doi: 10.3389/fpls.2017.00039. eCollection 2017.	Disruption of GLABROUS1 gene in Arabidopsis using CRISPR/Cas9 pUB-Cas9
	pTX168		Hygromycin	Voytas	A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.	Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants pCAMBIA1300 Tag / Fusion Protein SV40 NLS (C terminal on insert)
	pTX171		Hygromycin	Voytas	A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.	Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants pTX168 Tag / Fusion Protein SV40 NLS (C terminal on insert)
	pTX172		Hygromycin	Voytas	A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.	Express STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plants pTX171 Tag / Fusion Protein SV40 NLS (C terminal on insert)
	pTX176		Hygromycin	Voytas	A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.	Express estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plants pTX171 Tag / Fusion Protein SV40 NLS (C terminal on insert)
	pJG80	TaCas9 (Other)		Voytas	High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9. Plant J. 2016 Dec 10. doi: 10.1111/tpj.13446.	Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites unknown
	pMOD_A0108	AtCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP pMOD_A1001
	pMOD_A0502	Csy4-P2A-AtCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSP pMOD_A1001
	pMOD_A0508	Csy4-P2A-AtCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP pMOD_A1001
	pMOD_A0901	TREX2-P2A-AtCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: 35S, Gene: TREX2-P2A-AtCas9, Terminator: HSP pMOD_A1001
	pMOD_A0908	TREX2-P2A-AtCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP pMOD_A1001
	pMOD_A1110	TaCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP pMOD_A1001
	pMOD_A1111	TaCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP pMOD_A1001
	pMOD_A1510	Csy4-P2A-TaCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP pMOD_A1001
	pMOD_A1511	Csy4-P2A-TaCas9 (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP pMOD_A1001
	pDIRECT_21A	Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic)	Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II pTRANS_210
	pDIRECT_21C	Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic)	Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II pTRANS_210d
	pDIRECT_22A	Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic)	Kanamycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II pTRANS_220
	pDIRECT_22C	Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic)	Kanamycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II pTRANS_220d
	pDIRECT_23A	Engineering Reagent: 35S:AtCas9 + AtU6:gRNA (Synthetic)	Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar pTRANS_230
	pDIRECT_23C	Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers (Synthetic)	Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar pTRANS_230d
	pDIRECT_25F	Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA (Synthetic)	Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II pTRANS_250d
	pDIRECT_25H	Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers (Synthetic)	Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II pTRANS_250d
	pDIRECT_26H	Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers (Synthetic)	Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar pTRANS_260d
	pAtCas9_1	AtCas9 (Synthetic)	Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II pCAMBIA
	pAtCas9_3	AtCas9 (Synthetic)	Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar pCAMBIA
	pTaCas9_5	TaCas9 (Synthetic)	Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II pCAMBIA
	pTaCas9_6	TaCas9 (Synthetic)	Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar pCAMBIA
	pDIRECT_10A	35S:AtCas9 + AtU6:gRNA (Synthetic)		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none pTRANS_100
	pKEE401		Neomycin (select with G418)	Chen	Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.	CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance pCambia
	pXEE401B			Chen	Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.	CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance pCambia
	pXEE401BG			Chen	Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.	CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance pCambia
	pSC10			Stupar	CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula. Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.	pAH595-gRNA 2xplex entry cassette pENTR top
	pSC12			Stupar	CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula. Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.	pNB184-Cas9 entry cassette pENTR top
	pYLCRISPR/Cas9pUbi-N	Cas9 (Synthetic)		Liu	A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 10.1016/j.molp.2015.04.007.	Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection pCAMBIA1300
	pJKW0458			Weng	pJKW0457 (unpublished)	p35S:CAS9 pEarleyGate100
	pJKW0474			Weng	pJKW0457 (unpublished)	pCLV3:Cas9 pEarleyGate100
	pMpGE006	Atco-Cas9-Pea3ter (Synthetic)	Hygromycin	Sugano	Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.	Cas9 expression in M.polymorpha pMpGWB103
	pYPQ239	FnCpf1 (Other)		Qi	Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20.	FnCpf1 Gateway entry plasmid pYPQ167 Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
	pYPQ230-RR	LbCpf1-RR (LbCpf1 with G548R and K611R mutations) (Other)		Qi	Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20.	LbCpf1 Gateway entry plasmid with G548R and K611R mutations pYPQ167 Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
	pYPQ230-RVR	LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations) (Other)		Qi	Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20.	LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations pYPQ167 Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
	pYPQ239-RR	FnCpf1-RR (FnCpf1 with N623R and K687R mutations) (Other)		Qi	Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20.	FnCpf1 Gateway entry plasmid with N623R and K687R mutations pYPQ167 Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
	pYPQ239-RVR	FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations) (Other)		Qi	Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites. Mol Plant. 2018 Jul 2;11(7):999-1002. doi: 10.1016/j.molp.2018.03.008. Epub 2018 Mar 20.	FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations pYPQ167 Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
	pORFE0001	AtCAS9 (Other)		Chevalier	Chevalier Lab plasmids (unpublished)	AtCas9 module CDS1 pICH41308
	pORFE1001	AtCAS9 (Other)		Chevalier	Chevalier Lab plasmids (unpublished)	Overexpression of the AtCAS9 in plant under 2x35S promoter pICH47742
	pMH-Cas9-gate	Cas9 (Other)	Hygromycin	Bezanilla	Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.	Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants. pGEM-T easy
	pMK-Cas9-gate	Cas9 (Other)	Neomycin (select with G418)	Bezanilla	Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.	Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants. pGEM-T easy
	pZeo-Cas9-gate	Cas9 (Other)	Zeocin	Bezanilla	Efficient and modular CRISPR-Cas9 vector system for Physcomitrella patens. Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.	Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants. pGEM-T easy
	BCJJ339	BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI (Arabidopsis thaliana)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 pICH47742
	BCJJ340	BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI (Synthetic)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	UBI10:Cas9:E9 Golden Gate Level 1 Position 2 pICH47742
	BCJJ344	BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI (Synthetic)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse pICH47811
	BCJJ345	BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI (Synthetic)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse pICH47811
	BCJJ358	BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI (Synthetic)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse pICH47811
	pICSL90016	BsaI:AATG:Cas9_2:GCTT:BsaI (Synthetic)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	Cas9_2 Golden Gate Level 0 pICH41308
	BCJJ125	BsaI:AATG:Cas9_3(intron):GCTT:BsaI (Synthetic)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	Cas9_3 (with intron) Golden Gate Level 0 pICH41308
	BCJJ180	BsaI:AATG:Cas9_4:GCTT:BsaI (Synthetic)		Jones	Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis bioRxiv 419952	Cas9_4 Golden Gate Level 0 pICH41308
	pICSL90005	SpCas9-h (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SpCas9-h (no stop codon) pAGM1287
	pEPOR0SP0013	SpCas9-p (with stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SpCas9-p pICH41308
	pEPOR0SP0009	SpCas9-p (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SpCas9-p (no stop codon) pAGM1287
	pEPOR0SP0001	SpCas9-DE (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon) pAGM1287
	pEPOR0SP0014	SpCas9-KA (with stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SpCas9-KA pICH41308
	pEPOR0SP0015	SpCas9-KA (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon) pAGM1287
	pEPOR0SP0016	Sp-eCas9 1.0 (with stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, Sp-eCas9 1.0 pICH41308
	pEPOR0SP0017	Sp-eCas9 1.0 (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon) pAGM1287
	pEPOR0SP0018	Sp-eCas9 1.1 (with stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, Sp-eCas9 1.1 pICH41308
	pEPOR0SP0019	Sp-eCas9 1.1 (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon) pAGM1287
	pEPOR0SP0011	Sp-xCas9 3.7 (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon) pAGM1287
	pEPOR0SP0020	SaCas9 (with stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SaCas9 pICH41308
	pEPOR0SP0021	SaCas9 (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, SaCas9 (no stop codon) pAGM1287
	pEPOR0SP0012	eSaCas9 (no stop codon) (Other)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level0 Golden Gate part: CDS, eSaCas9 (no stop codon) pAGM1287
	pICSL11023	2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants pICH47742
	pEPOR1CB0002	2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants pICH47742
	pEPOR1CB0009	2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421) (Synthetic)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plants pICH47742
	pEPOR1CB0011	2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421) (Synthetic)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plants pICH47742
	pEPOR1CB0112	2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421) (Synthetic)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants pICH47742
	pEPOR1CB0015	2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421) (Synthetic)		Patron	Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. bioRxiv 422766	Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants pICH47742
	p221z-CAS9p-t35s	CAS9p (Other)		Mähönen	Plants CRISPR plasmids (unpublished)	Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system pDONR-221z
	p221z-CAS9p-TagRFP-t35s	CAS9p-TagRFP (Other)		Mähönen	Plants CRISPR plasmids (unpublished)	Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system pDONR-221z
	pYPQ150-x3.7	pcoCas9-x3.7		Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with pcoCas9-x3.7 (Plant codon optimized) pNJB91
	pGEL062		Hygromycin	Zhang	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	For genome editing in plant by SpCas9 wild type pZHY988
	pGEL063		Hygromycin	Zhang	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	For genome editing in plant by SpCas9 variant(SpCas9-NG) pZHY988
	pGEL065		Hygromycin	Zhang	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	For genome editing in plant by SpCas9 variant(SpCas9-NGv1) pZHY988
	pFH13	SpCas9, wheat codon optimized		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 SpCas9, wheat codon optimized pICH41308
	pFH14	SaCas9, Arabidopsis codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 SaCas9, Arabidopsis codon optimized pICH41308
	pFH15	SaCas9, Arabidopsis codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 StCas9, Arabidopsis codon optimized pICH41308
	pFH16	FnCas12a, rice codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 FnCas12a, rice codon optimized pICH41308
	pFH17	LbCas12a, rice codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 LbCas12a, rice codon optimized pICH41308
	pFH22	xCas9 3.7 (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 xCas9 3.7 pICH41308
	pFH32	SpCas9-NG, human codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 SpCas9-NG, human codon optimized pICH41308
	pFH46	FnCas12a, human codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 FnCas12a, human codon optimized pICH41308
	pFH47	LbCas12a, human codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 LbCas12a, human codon optimized pICH41308
	pFH48	Csy4 (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 Csy4 pICH41308
	pFH76	ScCas9, wheat codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 ScCas9, wheat codon optimized pICH41308
	pFH77	enCas9-PolI3M-TBD, wheat codon optimized (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 enCas9-PolI3M-TBD, wheat codon optimized pICH41308
	pRGEB32-BAR		Basta ; BAR	Hunter	CRISPR/Cas9 vector optimized for Agrobacterium-mediated transformation of maize (with BAR selectable marker) (modified from pRGEB32) (unpublished)	For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs. pRGEB32
	pENTR_Wheat_live_Cas9	Wheat_live_Cas9 (Synthetic)		Kamoun	Genome editing in wheat (unpublished)	Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules. pENTR4
	pICSL11085_GG_Wheat_live_Cas9_for	Wheat_live_Cas9 (Synthetic)		Kamoun	Genome editing in wheat (unpublished)	Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids. pICH47742
	pICSL11086_GG_Wheat_live_Cas9_rev	Wheat_live_Cas9 (Synthetic)		Kamoun	Genome editing in wheat (unpublished)	Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids. pICH47742
	pFH23	ZmUBIp::SpCas9 (wheat codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt pICH47742
	pFH52	2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt pICH47742
	pFH53	2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At pICH47742
	pFH54	AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At pICH47742
	pFH58	ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt pICH47742
	pFH59	ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt pICH47742
	pFH60	ZmUBIp::FnCas12a (rice codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::FnCas12a (rice codon optimized):NOSt pICH47742
	pFH61	ZmUBIp::LbCas12a (rice codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt pICH47742
	pFH68	ZmUBIp::SpCas9-NG (human codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt pICH47742
	pFH80	ZmUBIp::xCas9 3.7:NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::xCas9 3.7:NOSt pICH47742
	pFH81	ZmUBIp::ScCas9 (wheat codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt pICH47742
	pFH82	ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt pICH47742
	pARS3_MUbCAS9_GW		Hygromycin	Schaller	Type-B response regulators of rice play key roles in growth, development, and cytokinin signaling. Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870.	T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA pARS3 Tag / Fusion Protein HA
	pARS3_MUbCAS9_MC		Hygromycin	Nimchuk	Type-B response regulators of rice play key roles in growth, development, and cytokinin signaling. Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870.	T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA pARS3 Tag / Fusion Protein HA
	pFH24	SpCas9, wheat codon optimized, version 2 (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 SpCas9, wheat codon optimized (version 2) pICH41308
	pFH25	SpCas9, wheat codon optimized, version 3 (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 SpCas9, wheat codon optimized (version 3) pICH41308
	pFH66	ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt pICH47742
	pFH67	ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt (Synthetic)		Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt pICH47742
	pG3GB411	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3H-U3Ub	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3B-U3Ub	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3B-U6SC	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3K-U6SC	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3H-U6SC	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3B-U6EC1	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3K-U6EC1	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pG3H-U6EC1	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pGreen3 CRISPR/Cas9 pGreen3
	pGB411	CRISPR/Cas9 (Synthetic)		Chen	A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767.	pCambia CRISPR/Cas9 pCambia
	pGGA_RT_Sa_Cas9	SaCAS9 (Synthetic)		Theres	Theres lab: Alternative Greengate/Goldengate modules (unpublished)	Goldengate/Greengate module for genome editing, using the SaCAS9. pUC19
	pGEL077			Zhang	Yong Zhang lab plasmids (unpublished)	iSTU-CRISPR/Cas9 with StIV2 intron, NU structure backbone pZHY988
	pGEL078			Zhang	Yong Zhang lab plasmids (unpublished)	iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone pZHY988
	pGEL079			Zhang	Yong Zhang lab plasmids (unpublished)	iSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backbone pZHY988
	pGEL081			Zhang	Yong Zhang lab plasmids (unpublished)	iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone pZHY988
	pGEL082			Zhang	Yong Zhang lab plasmids (unpublished)	iSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backbone pZHY988
	pGEL083			Zhang	Yong Zhang lab plasmids (unpublished)	iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone pZHY988
	pGEL085			Zhang	Yong Zhang lab plasmids (unpublished)	iSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backbone pZHY988
	pGEL029			Zhang	Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17.	STU2.0 SpyCas9 RZ system for plant genome editing pZHY988
	pGEL031			Zhang	Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17.	STU2.0 SpyCas9 tRNA system for plant genome editing pZHY988
	pGEL033			Zhang	Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17.	STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing pZHY988
	pGEL050			Zhang	Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing. Front Plant Sci. 2019 Sep 20;10:1173. doi: 10.3389/fpls.2019.01173. eCollection 2019.	Dual-mini35S driven Cas9-Csy4 system pTX152
	pGEL051			Zhang	Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing. Front Plant Sci. 2019 Sep 20;10:1173. doi: 10.3389/fpls.2019.01173. eCollection 2019.	Dual-mini35S-enhancer driven Cas9-Csy4 system pTX152

Base Edit

Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations.

	Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
	pMonAID_dCas9-PmCDA_Hyg_ALS	SpCas9 (Other)	2x35S	Hygromycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
	pMonAID_nCas9-PmCDA_Hyg_ALS	SpCas9 (Other)	2x35S	Hygromycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
	pDicAID_nCas9-PmCDA_NptII_Della	SpCas9 (Other)	PcUbi	Kanamycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella pZD_OsU3gYSA_HolgerCas9_NPTII
	pDicAID_nCas9-PmCDA-2A-NptII_ETR	SpCas9 (Other)	PcUbi	Kanamycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr pZD_OsU3gYSA_HolgerCas9_NPTII
	pHEE901			Hygromycin	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR pCambia
	pHSE901			Hygromycin	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR pCambia
	pBSE901			Neomycin (select with G418)	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance pCambia
	pXSE901B			Basta	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance pCambia
	pXSE901BG			Basta ; Glyphosate	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance pCambia
	pB-dCas9-PBE	NLS-APOBEC1-XTEN-dCas9-UGI-NLS	Ubi	Basta	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in maize pBUE411
	pB-nCas9-PBE	NLS-APOBEC1-XTEN-nCas9-UGI-NLS	Ubi	Basta	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in maize pBUE411
	pdCas9-PBE	NLS-APOBEC1-XTEN-dCas9-UGI-NLS	Ubi		Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
	pH-dCas9-PBE	NLS-APOBEC1-XTEN-dCas9-UGI-NLS	Ubi	Hygromycin	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in rice pHUE411
	pH-nCas9-PBE	NLS-APOBEC1-XTEN-nCas9-UGI-NLS	Ubi	Hygromycin	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in rice pHUE411
	pnCas9-PBE	NLS-APOBEC1-XTEN-nCas9-UGI-NLS	Ubi		Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
	pJY-RpABE	RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA cassette		Hygromycin	Kim	Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x.	binary vector for ABE expression in Arabidopsis pKI1.1R
	pJY-RpABE-FT_INF	RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting FT		Hygromycin	Kim	Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x.	binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis pJY-RpABE
	pJY-RpABE-PDS3_IMS	RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting PDS3		Hygromycin	Kim	Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x.	binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis pJY-RpABE
	pH-PABE-7-esgRNA	wtTadA-TadA7.10-nCas9-3*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in rice pCAMBIA1300
	pH-PABE-7-sgRNA	wtTadA-TadA7.10-nCas9-3*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in rice pCAMBIA1300
	PABE-1	NLS-wtTadA-TadA7.10-nCas9-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
	PABE-2	wtTadA-TadA7.10-nCas9-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
	PABE-3	wtTadA-TadA7.10-NLS-nCas9-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
	PABE-4	NLS-nCas9-wtTadA-TadA7.10-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
	PABE-5	NLS-nCas9-NLS-wtTadA-TadA7.10-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
	PABE-6	wtTadA-TadA7.10-nCas9-2*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
	PABE-7	wtTadA-TadA7.10-nCas9-3*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
	pYPQ255	rAPOBEC1-pcoCas9D10A-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing pNJB91
	pYPQ255-x3.7	rAPOBEC1-pcoCas9D10A_x3.7-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
	pYPQ256	pcoCas9D10A-PmCDA1-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing pNJB91
	pYPQ256-x3.7	pcoCas9D10A_x3.7-PmCDA1-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
	pGEL066			Hygromycin	Zhang	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor pZHY988 Tag / Fusion Protein 3xFlag (C terminal on backbone)
	pFH45	nSpCas9-ABE7.10 base changer, human codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 nSpCas9-ABE7.10 base changer, human codon optimized pICH41308
	pFH55	nSpCas9-PmCDA, Arabidopsis codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 nSpCas9-PmCDA, Arabidopsis codon optimized pICH41308
	pFH79	A3A-PBE, wheat codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 A3A-PBE, wheat codon optimized pICH41308
	pFH92	nScCas9-ABE7.10, wheat/human codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 nScCas9-ABE7.10, wheat/human codon optimized pICH41308
	pFH70	ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt pICH47742
	pFH84	ZmUBIp::A3A-PBE (wheat codon optimized):NOSt (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt pICH47742
	pFH93	ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt pICH47742
	pGEL033				Zhang	Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17.	STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing pZHY988

Nick

CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR).

	Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
	pBUN501	zCas9D10A (Synthetic), gRNA scaffold (Synthetic)	Ubi1p, AtU6-26p	Bar	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance pGreen-like binary vector
	pHSN501	zCas9D10A (Synthetic), gRNA scaffold (Synthetic)	2×35Sp, AtU6-26p	Hygromycin	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
	pDe-CAS9-D10A	Cas9-D10A (Arabidopsis thaliana)	PcUbi4-2	Kanamycin	Puchta	Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.	based on pDe-CAS9, encodes Cas9 D10A nickase variant pPZP221
	pYPQ159	hSpCas9D10A (Human codon optimized) (Other)			Qi	A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.	Gateway entry vector with hSpCas9D10A pNJB185-linker6
	pDGE76	pnos:nptII-tmos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI			Stuttmann	Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.	recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII pVM_BGW
	pDGE77	pnos:PAT-tnos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI			Stuttmann	Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.	recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA pVM_BGW
	pDGE78	pnos:nptII-tmos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI			Stuttmann	Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.	recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII pVM_BGW
	pDGE79	pnos:PAT-tnos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI			Stuttmann	Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.	recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA pVM_BGW
	pTX179			Hygromycin	Voytas	A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants. Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.	Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants pTX172 Tag / Fusion Protein SV40 NLS (C terminal on insert)
	pMOD_A0201	AtCas9_D10A nickase (Synthetic)	35S		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0202	AtCas9_D10A nickase (Synthetic)	AtUbi10		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0208	AtCas9_D10A nickase (Synthetic)	EC1.2		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0301	AtCas9_H840A nickase (Synthetic)	35S		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0302	AtCas9_H840A nickase (Synthetic)	AtUbi10		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0308	AtCas9_H840A nickase (Synthetic)	EC1.2		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0601	Csy4-P2A-AtCas9_D10A nickase (Synthetic)	35S		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0608	Csy4-P2A-AtCas9_D10A nickase (Synthetic)	EC1.2		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0701	Csy4-P2A-AtCas9_H840A nickase (Synthetic)	35S		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
	pMOD_A0708	Csy4-P2A-AtCas9_H840A nickase (Synthetic)	EC1.2		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP pMOD_A1001
	pMOD_A1210	TaCas9_D10A nickase (Synthetic)	ZmUbi		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSP pMOD_A1001
	pMOD_A1310	TaCas9_H840A nickase (Synthetic)	ZmUbi		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP pMOD_A1001
	pMOD_A1610	Csy4-P2A-TaCas9_D10A nickase (Synthetic)	ZmUbi		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP pMOD_A1001
	pMOD_A1710	Csy4-P2A-TaCas9_H840A nickase (Synthetic)	ZmUbi		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP pMOD_A1001
	pAtCas9-D10A_1	AtCas9_D10A (nickase) (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II pCAMBIA
	pAtCas9-D10A_2	AtCas9_D10A (nickase) (Synthetic)		Kanamycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II pCAMBIA
	pAtCas9-D10A_3	AtCas9_D10A (nickase) (Synthetic)		Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar pCAMBIA
	pAtCas9-H840A_1	AtCas9_H840A (nickase) (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II pCAMBIA
	pAtCas9-H840A_2	AtCas9_H840A (nickase) (Synthetic)		Kanamycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II pCAMBIA
	pTaCas9-D10A_5	TaCas9_D10A (nickase) (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II pCAMBIA
	pTaCas9-D10A_6	TaCas9_D10A (nickase) (Synthetic)		Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar pCAMBIA
	pTaCas9-H840A_5	TaCas9_H840A (nickase) (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II pCAMBIA
	pTaCas9-H840A_6	TaCas9_H840A (nickase) (Synthetic)		Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar pCAMBIA
	pJG363	nAtCas9_D10A+gNt_R2+donor (Other)			Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor) pTRANS_201
	pJG367	nAtCas9_H840A+gNt_R2+gNt_F2+donor (Other)			Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor) pTRANS_201
	pJG370	nAtCas9_D10A+gRNActrl+donor (Synthetic)			Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor) pTRANS_201
	pJG382	nAtCas9_D10A+gNt_R2+gNt_F2+donor (Other)			Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor) pTRANS_201
	pJG484	nTaCas9_H840A+gUbi8+donor (Other)			Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor) pTRANS_203
	pJG486	nTaCas9_H840A+gUbi1+donor (Other)			Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor) pTRANS_203
	pJG488	nTaCas9_H840A+gUbi8+gUbi1+donor (Other)			Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor) pTRANS_203
	pORFE0002	AtCAS9 nickase D10A (Other)			Chevalier	Chevalier Lab plasmids (unpublished)	AtCAS9 nickase D10A module CDS1 pICH41308
	pORFE0014	AtCAS9 D10A without stop codon (Other)			Chevalier	Chevalier Lab plasmids (unpublished)	AtCAS9 nickase D10A module CDS1ns pAGM1287
	pORFE1052	AtCAS9 D10A (Other)	2x35S		Chevalier	Chevalier Lab plasmids (unpublished)	Overexpression of the AtCAS9 nickase in plant under 2x35S promoter pICH47742
	pFH91	nScCas9 (nickase), wheat codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level0 nScCas9 (nickase), wheat codon optimized pICH41308
	pFH109	ZmUBIp::nScCas9 (wheat codon optimized):NOSt (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants bioRxiv	Level1 ZmUBIp::nScCas9 (wheat codon optimized):NOSt pICH47742

Activate

Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus.

	Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
	pBUN6A11	dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)	Ubi1p, OsU3p	Bar	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
	pHSN6A01	dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)	2×35Sp, AtU6-26p	Hygromycin	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
	pYPQ152	pco-dCas9-VP64 (Plant codon-optimized) (Other)			Qi	A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.	Gateway entry vector with pco-dCas9-VP64 pYPQ185-linker1
	pYPQ173	pco-dCas9-VP64-T2A-MS2-VP64 (Synthetic)			Qi	Robust transcriptional activation in plants using multiplexed CRISPR-Act2.0 and mTALE-Act systems. Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 10.1016/j.molp.2017.11.010.	Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence unknown
	pEG302 5aa SunTag VP64 nog	NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS		Hygromycin	Jacobsen	Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.	CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) pEG302
	pEG302 22aa SunTag NtDRMcd nog	NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS		Hygromycin	Jacobsen	Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.	CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide) pEG302
	pEG302 22aa SunTag NtDRMcd (noNLS) nog	NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS		Hygromycin	Jacobsen	Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.	CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide) pEG302
	pEG302 22aa SunTag VP64 nog	NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS		Hygromycin	Jacobsen	Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.	CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide) pEG302
	PV225	dCas9-AT-CBF1 (Other)			Young	Repurposing of Type I-E CRISPR-Cascade for gene activation in plants Nature Communications Biology	dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays PHP17720 Tag / Fusion Protein AT-CBF1 (C terminal on insert) CBF1 AT4G25490, ATCBF1, C-repeat/DRE binding factor 1, DRE BINDING PROTEIN 1B, DREB1B, T30C3.11, TRANSCRIPTIONAL ACTIVATOR CBF1
	pYPQ239A (dFnCas12a)-TV	dFnCas12a-TV (Other)			Qi	CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020)	CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system pYPQ167
	pYPQ292 (AaCas12b)-D570A-TV	AaCas12b (D570A)-TV (Other)			Qi	CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020)	CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system pYPQ167
	pYPQ292 (AaCas12b)-D570A-TV-MS2-TV	AaCas12b (D570A)-TV-T2A-MCP-TV (Other)			Qi	CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020)	CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein pYPQ167
	pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR	AaCas12b (D570A)-TV-T2A-MCP-VPR (Other)			Qi	CRISPR–Cas12b enables efficient plant genome engineering Nature Plants (2020)	CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein pYPQ167

Interfere

Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer or the beginning of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus.

	Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
	pBUN6I11	dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic)	Ubi1p, OsU3p	Bar	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
	pHSN6I01	dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic)	2×35Sp, AtU6-26p	Hygromycin	Chen	A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327.	CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
	pEGB 35S:dCas9:Tnos (GB1191)	dCas9 (Other)	35S		Orzaez	GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol. 2013 Jul;162(3):1618-31.	Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter pDGB3alpha2
	pYPQ153	pco-dCas9-3X(SRDX) (Plant codon-optimized) (Other)			Qi	A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.	Gateway entry vector with pco-dCas9-3X(SRDX) pYPQ185-linker1
	pdCas9 (GB1079)	Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised) (Other)			Orzaez	A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.	Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions pUPD
	pEGB 35s:dCas:BRD:tNos (GB1172)	dCas9:BRD (Synthetic)	35S		Orzaez	A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016.	Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor pDGB3alpha2
	pYPQ223 (dAsCpf1-SRDX)	dAsCpf1-SRDX (Other)			Qi	A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.	Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid pYPQ221
	pYPQ233 (dLbCpf1-SRDX)	LbCpf1-SRDX (Other)			Qi	A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.	Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid pYPQ221
	pMOD_A0402	AtCas9_dead (D10A + H840A) (Synthetic)	AtUbi10		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
	pMOD_A0408	AtCas9_dead (D10A + H840A) (Synthetic)	EC1.2		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
	pMOD_A0801	Csy4-P2A-AtCas9_dead (D10A + H840A) (Synthetic)	35S		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
	pMOD_A0802	Csy4-P2A-AtCas9_dead (D10A + H840A) (Synthetic)	AtUbi10		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
	pMOD_A0808	Csy4-P2A-AtCas9_dead (D10A + H840A) (Synthetic)	EC1.2		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
	pMOD_A1410	TaCas9_dead (D10A + H840A) (Synthetic)	ZmUbi		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
	pMOD_A1810	Csy4-P2A-TaCas9_dead (D10A + H840A) (Synthetic)	ZmUbi		Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP pMOD_A1001
	pDIRECT_21B	Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II pTRANS_210
	pDIRECT_25G	Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II pTRANS_250d
	pDIRECT_26G	Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA (Synthetic)		Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar pTRANS_260d
	pAtCas9-dead_1	AtCas9_D10A+H840A (dead) (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II pCAMBIA
	pAtCas9-dead_2	AtCas9_D10A+H840A (dead) (Synthetic)		Kanamycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II pCAMBIA
	pAtCas9-dead_3	AtCas9_D10A+H840A (dead) (Synthetic)		Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar pCAMBIA
	pTaCas9-dead_5	TaCas9_D10A+H840A (dead) (Synthetic)		Hygromycin	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II pCAMBIA
	pTaCas9-dead_6	TaCas9_D10A+H840A (dead) (Synthetic)		Basta	Voytas	A multi-purpose toolkit to enable advanced genome engineering in plants. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.	Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:bar pCAMBIA
	pHdzCas9-KRAB		CaMV35S	Hygromycin	Ng	CRISPRi mediated phosphoenolpyruvate carboxylase regulation to enhance the production of lipid in Chlamydomonas reinhardtii. Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30619-3. doi: 10.1016/j.biortech.2017.04.111.	Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance pHdzCas9-KRAB Tag / Fusion Protein KRAB (C terminal on insert)
	pORFE0003	AtDeadCAS9 D10A H840A (Other)			Chevalier	Chevalier Lab plasmids (unpublished)	AtDeadCAS9 D10A H840A module CDS1 pICH41308
	pORFE0005	AtDeadCAS9 D10A H840A without stop codon (Other)			Chevalier	Chevalier Lab plasmids (unpublished)	AtDeadCAS9 D10A H840A without stop codon module CDS1ns pAGM1287
	p221z-dCAS9p-t35s	dCAS9p (Other)			Mähönen	Plants CRISPR plasmids (unpublished)	Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system pDONR-221z

Empty gRNA Expression Vectors

Select a gRNA expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids.

	Plasmid	Promoter	Cloning Enzyme	Co-expressed Cas9	Cas9 System	Selection	PI
	pICSL01009::AtU6p	aU6	BsaI	none	S. pyogenes		Kamoun
	pUC119-gRNA	U6	PCR template	none	S. pyogenes		Sheen
	pRGEB31	rice snoRNA U3	BsaI	none	S. pyogenes		Yang
	pBUN6A11	OsU3	BsaI	yes, activate	S. pyogenes	Bar	Chen
	pBUN6I11	OsU3	BsaI	yes, interfere	S. pyogenes	Bar	Chen
	pBUN501	AtU6-26	BsaI	yes, nick	S. pyogenes	Bar	Chen
	pCBC-MT2T3	see paper	BsaI	none	S. pyogenes		Chen
	pCBC-MT3T4	see paper	BsaI	none	S. pyogenes		Chen
	pBUN421	TaU3	BsaI	yes, cut	S. pyogenes	Bar	Chen
	pU3-gRNA	OsU3	AarI	none	S. pyogenes		Gao
	pZmU3-gRNA	maize U3		none	S. pyogenes		Gao
	pU6-gRNA	wheat U6	BbsI	none	S. pyogenes		Gao
	pBlu/gRNA	Arabidopsis U6	BbsI	none	S. pyogenes		Stupar
	pBUE411	OsU3		yes, cut	S. pyogenes	Basta	Chen
	pHUE411	OsU3		yes, cut	S. pyogenes	Hyg	Chen
	pHAtC	U6	AarI	yes, cut	S. pyogenes	Hygro	Kim
	pBAtC	U6	AarI	yes, cut	S. pyogenes	Basta	Kim
	pHEE401	U6-26p	BsaI	yes, cut	S. pyogenes	Hygro	Chen
	pHEE401E	U6-26p	BsaI	yes, cut	S. pyogenes	Hygro	Chen
	pHDE-35S-Cas9-mCherry-UBQ	U6-26	PmeI	yes, cut	S. pyogenes	Hygro, mCherry	Zhao
	pCBC-DT1T1	see paper	BsaI	none	S. pyogenes		Chen
	pRGEB32	rice snoRNA U3	BsaI	yes, cut	S. pyogenes	Hygro	Yang
	pHSE401	AtU6-26		yes, cut	S. pyogenes	Hyg	Chen
	pBUN411	OsU3	BsaI	yes, cut	S. pyogenes	Bar	Chen
	pHSN6A01	AtU6-26	BsaI	yes, activate	S. pyogenes	Hyg	Chen
	pHSN6I01	AtU6-26	BsaI	yes, interfere	S. pyogenes	Hyg	Chen
	pHSN501	AtU6-26	BsaI	yes, nick	S. pyogenes	Hyg	Chen
	pCBC-DT3T4	see paper	BsaI	none	S. pyogenes		Chen
	pCBC-MT1T2	see paper	BsaI	none	S. pyogenes		Chen
	pRGE32	rice snoRNA U3	BsaI	yes, cut	S. pyogenes	Hygro	Yang
	pKSE401	AtU6-26		yes, cut	S. pyogenes	Kan	Chen
	pRGE31	rice snoRNA U3	BsaI	none	S. pyogenes		Yang
	pUC gRNA Shuttle	Mt U6.6	inFusion	none	S. pyogenes		Parrott
	pCBC-DT2T3	see paper	BsaI	none	S. pyogenes		Chen
	pHSN401	AtU6-26	BsaI	yes, cut	S. pyogenes	Hyg	Chen
	pYPQ141-ZmUbi-RZ-As	Maize ubiquitin 1		none	As Cpf1		Qi
	pKEE401			yes, cut	S. pyogenes	Neomycin	Chen