1426 LOCUS ADDGENE_1426 3052 bp DNA circular SYN 01/03/2005 DEFINITION pAST_18b complete plasmid sequence KEYWORDS . SOURCE pAST_18b ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1426 at www.addgene.org FEATURES Location/Qualifiers source 1..3052 /organism="Addgene plasmid pAST_18b" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..393) /label="lacZ_a" misc_feature complement(461..479) /label="M13r_primer" promoter complement(514..543) /label="lac_promoter" misc_feature complement(610..636) /label="lacI.C" rep_origin complement(852..1471) /label="pBR322_origin" CDS complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" misc_feature complement(2778..2807) /label="SupTrp.3'" misc_feature complement(2822..2851) /label="SupTrp.5'" promoter complement(2894..2922) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTGCATGC CTGCAGGTCG 421 ACTCTAGAGG ATCCCCGGGT ACCGAGCTCG AATTCGTAAT CATGGTCATA GCTGTTTCCT 481 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 541 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 601 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 661 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 721 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 781 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 841 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 901 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 961 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 1021 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT 1081 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 1141 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 1201 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 1261 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT 1321 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 1381 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 1441 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 1501 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 1561 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 1621 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 1681 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 1741 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 1801 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 1861 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 1921 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 1981 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2041 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2101 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2161 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2221 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2281 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 2341 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 2401 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 2461 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATACAATTT TCAGAATACG 2521 TTTTTTGTGG GCTTGGGTAT ATTGTTTTTA ATGTTATACT TGCAGTCGTG AAATTTGATT 2581 TTCAAATTTG TAGAAAAATC AAGAAAATAA TTGCAACATT CGCTTGTGTC AAAAACCAAT 2641 TTCAACAAAT TTTCGTGTGA GAAATACATT ACCAGAAGGC ATTTTTTCAC ACGATTAGCA 2701 TTTTGGACTA CTTTATTAAA TTTTTGCGTG TAATTTTGAA TTAAATTGTA TTATATTACT 2761 ACTTAAAAAA CAAAAAATTT GACCACTGAG CGGATCGAAC GCCCAACCTT TCGATCTAGA 2821 GTCGAACGCG CTACCATTGC GCCAAGCAGT CATGTTATTC TCTCTTGTCA TTATTGAAGC 2881 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 2941 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT 3001 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC // 1427 LOCUS ADDGENE_1427 3052 bp DNA circular SYN 01/03/2005 DEFINITION pAST_19a complete plasmid sequence KEYWORDS . SOURCE pAST_19a ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1427 at www.addgene.org FEATURES Location/Qualifiers source 1..3052 /organism="Addgene plasmid pAST_19a" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..398) /label="lacZ_a" misc_feature complement(461..479) /label="M13r_primer" promoter complement(514..543) /label="lac_promoter" misc_feature complement(610..636) /label="lacI.C" rep_origin complement(852..1471) /label="pBR322_origin" gene complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" CDS complement(1626..2486) /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature complement(2778..2807) /label="SupTrp.3'" misc_feature complement(2822..2851) /label="SupTrp.5'" promoter complement(2894..2922) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGT ACCCGGGGAT 421 CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 481 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 541 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 601 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 661 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 721 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 781 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 841 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 901 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 961 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 1021 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 1081 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 1141 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 1201 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 1261 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT 1321 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 1381 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 1441 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 1501 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 1561 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 1621 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 1681 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 1741 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 1801 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 1861 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 1921 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 1981 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2041 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2101 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2161 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2221 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2281 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 2341 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 2401 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 2461 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATACAATTT TCAGAATACG 2521 TTTTTTGTGG GCTTGGGTAT ATTGTTTTTA ATGTTATACT TGCAGTCGTG AAATTTGATT 2581 TTCAAATTTG TAGAAAAATC AAGAAAATAA TTGCAACATT CGCTTGTGTC AAAAACCAAT 2641 TTCAACAAAT TTTCGTGTGA GAAATACATT ACCAGAAGGC ATTTTTTCAC ACGATTAGCA 2701 TTTTGGACTA CTTTATTAAA TTTTTGCGTG TAATTTTGAA TTAAATTGTA TTATATTACT 2761 ACTTAAAAAA CAAAAAATTT GACCACTGAG CGGATCGAAC GCCCAACCTT TCGATCTAGA 2821 GTCGAACGCG CTACCATTGC GCCAAGCAGT CATGTTATTC TCTCTTGTCA TTATTGAAGC 2881 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 2941 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT 3001 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC // 1428 LOCUS ADDGENE_1428 5808 bp DNA circular SYN 01/03/2005 DEFINITION pDD16_01 complete plasmid sequence KEYWORDS . SOURCE pDD16_01 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1428 at www.addgene.org FEATURES Location/Qualifiers source 1..5808 /organism="Addgene plasmid pDD16_01" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" promoter 3722..3750 /label="Amp_promoter" gene 3792..4652 /label="Ampicillin" /gene="Ampicillin" CDS 3792..4652 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4807..5426 /label="pBR322_origin" misc_feature 5642..5668 /label="lacI.C" promoter 5735..5764 /label="lac_promoter" misc_feature 5799..5817 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG 3361 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG 3421 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG 3481 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 3541 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG 3601 GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT 3661 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 3721 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA 3781 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT 3841 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC 3901 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 3961 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 4021 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC 4081 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG 4141 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC 4201 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 4261 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 4321 ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC 4381 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC 4441 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 4501 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG 4561 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 4621 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC 4681 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 4741 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 4801 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 4861 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 4921 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT 4981 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 5041 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 5101 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 5161 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG 5221 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 5281 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 5341 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 5401 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 5461 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 5521 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG 5581 AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT 5641 AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA 5701 ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA 5761 TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCT // 1429 LOCUS ADDGENE_1429 3042 bp DNA circular SYN 01/03/2005 DEFINITION pICT19h complete plasmid sequence KEYWORDS . SOURCE pICT19h ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1429 at www.addgene.org FEATURES Location/Qualifiers source 1..3042 /organism="Addgene plasmid pICT19h" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..393) /label="lacZ_a" misc_feature complement(700..729) /label="SupTrp.3'" misc_feature complement(744..773) /label="SupTrp.5'" misc_feature complement(817..835) /label="M13r_primer" promoter complement(870..899) /label="lac_promoter" misc_feature complement(966..992) /label="lacI.C" rep_origin complement(1208..1827) /label="pBR322_origin" CDS complement(1982..2842) /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene complement(1982..2842) /label="Ampicillin" /gene="Ampicillin" promoter complement(2884..2912) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTTCGCGA GCTCGAGATC 421 TAGATACAAT TTTCAGAATA CGTTTTTTGT GGGCTTGGGT ATATTGTTTT TAATGTTATA 481 CTTGCAGTCG TGAAATTTGA TTTTCAAATT TGTAGAAAAA TCAAGAAAAT AATTGCAACA 541 TTCGCTTGTG TCAAAAACCA ATTTCAACAA ATTTTCGTGT GAGAAATACA TTACCAGAAG 601 GCATTTTTTC ACACGATTAG CATTTTGGAC TACTTTATTA AATTTTTGCG TGTAATTTTG 661 AATTAAATTG TATTATATTA CTACTTAAAA AACAAAAAAT TTGACCACTG AGCGGATCGA 721 ACGCCCAACC TTTCGATCTA GAGTCGAACG CGCTACCATT GCGCCAAGCA GTCATGTTAT 781 TCTCTCTTGT CGACCTGCAG CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 841 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 901 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 961 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 1021 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 1081 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 1141 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 1201 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 1261 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 1321 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 1381 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 1441 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 1501 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 1561 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 1621 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 1681 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 1741 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 1801 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 1861 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 1921 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 1981 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 2041 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 2101 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 2161 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 2221 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 2281 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 2341 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 2401 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 2461 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 2521 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 2581 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 2641 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 2701 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 2761 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 2821 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 2881 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 2941 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA 3001 CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC // 1430 LOCUS ADDGENE_1430 3023 bp DNA circular SYN 01/03/2005 DEFINITION pICT19r complete plasmid sequence KEYWORDS . SOURCE pICT19r ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1430 at www.addgene.org FEATURES Location/Qualifiers source 1..3023 /organism="Addgene plasmid pICT19r" /mol_type="other DNA" misc_feature 379..395 /label="M13f20_primer" misc_feature complement(250..398) /label="lacZ_a" misc_feature 433..462 /label="SupTrp.5'" misc_feature 477..506 /label="SupTrp.3'" misc_feature complement(798..816) /label="M13r_primer" promoter complement(851..880) /label="lac_promoter" misc_feature complement(947..973) /label="lacI.C" rep_origin complement(1189..1808) /label="pBR322_origin" gene complement(1963..2823) /label="Ampicillin" /gene="Ampicillin" CDS complement(1963..2823) /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" promoter complement(2865..2893) /label="Amp_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCCGGGGATC CGTCGACAAG 421 AGAGAATAAC ATGACTGCTT GGCGCAATGG TAGCGCGTTC GACTCTAGAT CGAAAGGTTG 481 GGCGTTCGAT CCGCTCAGTG GTCAAATTTT TTGTTTTTTA AGTAGTAATA TAATACAATT 541 TAATTCAAAA TTACACGCAA AAATTTAATA AAGTAGTCCA AAATGCTAAT CGTGTGAAAA 601 AATGCCTTCT GGTAATGTAT TTCTCACACG AAAATTTGTT GAAATTGGTT TTTGACACAA 661 GCGAATGTTG CAATTATTTT CTTGATTTTT CTACAAATTT GAAAATCAAA TTTCACGACT 721 GCAAGTATAA CATTAAAAAC AATATACCCA AGCCCACAAA AAACGTATTC TGAAAATTGT 781 ATCGATGAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 841 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 901 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 961 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 1021 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 1081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 1141 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 1201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1621 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 2041 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 2101 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 2821 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 2881 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 2941 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 3001 GCGTATCACG AGGCCCTTTC GTC // 1431 LOCUS ADDGENE_1431 7236 bp DNA circular SYN 01/03/2005 DEFINITION pOK11_34 complete plasmid sequence KEYWORDS . SOURCE pOK11_34 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1431 at www.addgene.org FEATURES Location/Qualifiers source 1..7236 /organism="Addgene plasmid pOK11_34" /mol_type="other DNA" misc_feature 49..73 /label="myo2.delta-90" misc_feature 140..163 /label="myo2.5'" misc_feature 237..278 /label="SynIVS.A" CDS 315..3479 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 315..341 /label="SV40.NLS" gene 432..3476 /label="lacZ" /gene="lacZ" promoter 5150..5178 /label="Amp_promoter" CDS 5220..6080 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5220..6080 /label="Ampicillin" /gene="Ampicillin" rep_origin 6235..6854 /label="pBR322_origin" misc_feature 7070..7096 /label="lacI.C" promoter 7163..7192 /label="lac_promoter" misc_feature 7227..7245 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGCCTT GGTCGACTCT AGACACTTTT 61 CAGCTACCTA GATACATGGA TATCCCCGCC TCCCAATCCA CCCACCCAGG GAAAAAGAAG 121 GGCTCGCCGA AAATCAAAGT TATCTCCAGG CTCGCGCATC CCACCGAGCG GTTGACTTCT 181 CTCCACCACT TTTCATTTTA ACCCTCGGGG TACGGGATTG GCCAAAGGAC CCAAAGGTAT 241 GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCcttCT TGGAGGGTAC 301 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGTG GGTGAAGACC 361 AGAAACAGCA CCTCGAACTG AGCCGCGATA TTGCCCAGCG TTTCAACGCG CTGTATGGCG 421 AGATCGATCC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 481 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 541 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCTTTGCC TGGTTTCCGG 601 CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC GATACTGTCG 661 TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC AACGTGACCT 721 ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT TGTTACTCGC 781 TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT ATTTTTGATG 841 GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC GGCCAGGACA 901 GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA AACCGCCTCG 961 CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT ATGTGGCGGA 1021 TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA ATCAGCGATT 1081 TCCATGTTGC CACTCGCTTT AATGATGATT TCAGCCGCGC TGTACTGGAG GCTGAAGTTC 1141 AGATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT TTCTTTATGG CAGGGTGAAA 1201 CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT TATCGATGAG CGTGGTGGTT 1261 ATGCCGATCG CGTCACACTA CGTCTGAACG TCGAAAACCC GAAACTGTGG AGCGCCGAAA 1321 TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC CGACGGCACG CTGATTGAAG 1381 CAGAAGCCTG CGATGTCGGT TTCCGCGAGG TGCGGATTGA AAATGGTCTG CTGCTGCTGA 1441 ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA GCATCATCCT CTGCATGGTC 1501 AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATATCCTGCT GATGAAGCAG AACAACTTTA 1561 ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG TGCGACCGCT 1621 ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG CCAATGAATC 1681 GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG CGAATGGTGC 1741 AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGAA TCAGGCCACG 1801 GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT CGATCCTTCC CGCCCGGTGC 1861 AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT TATTTGCCCG ATGTACGCGC 1921 GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG GTCCATCAAA AAATGGCTTT 1981 CGCTACCTGG AGAGACGCGC CCGCTGATCC TTTGCGAATA CGCCCACGCG ATGGGTAACA 2041 GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT TTACAGGGCG 2101 GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC GGCAACCCGT 2161 GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACGA TCGCCAGTTC TGTATGAACG 2221 GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCGCTGAC GGAAGCAAAA CACCAGCAGC 2281 AGTTTTTCCA GTTCCGTTTA TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC 2341 GTCATAGCGA TAACGAGCTC CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA 2401 GCGGTGAAGT GCCTCTGGAT GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC 2461 TACCGCAGCC GGAGAGCGCC GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG 2521 CGACCGCATG GTCAGAAGCC GGGCACATCA GCGCCTGGCA GCAGTGGCGT CTGGCGGAAA 2581 ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC ACCAGCGAAA 2641 TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG TCAGGCTTTC 2701 TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC GATCAGTTCA 2761 CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT GACCCTAACG 2821 CCTGGGTCGA ACGCTGGAAG GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT 2881 GCACGGCAGA TACACTTGCT GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC 2941 AGGGGAAAAC CTTATTTATC AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG 3001 CGATTACCGT TGATGTTGAA GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA 3061 ACTGCCAGCT GGCGCAGGTA GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA 3121 ACTATCCCGA CCGCCTTACT GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA 3181 TGTATACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG CTGCGGGACG CGCGAATTGA 3241 ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA CATCAGCCGC TACAGTCAAC 3301 AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC GGAAGAAGGC ACATGGCTGA 3361 ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC CTGGAGCCCG TCAGTATCGG 3421 CGGAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT TGTCTGGTGT CAAAAATAAT 3481 AGGGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 3541 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 3601 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 3661 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 3721 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 3781 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 3841 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 3901 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 3961 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 4021 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 4081 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 4141 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 4201 TACATTCCCA ATATACCAAA CATAACTGTT TAAAATTAAA CATTTTTCTA AATTTTATAT 4261 GATTTCTTTT AAATTTGCAA AAATTACTTA AATTTGAATT CCCGCGCAAA TGAGTGACTT 4321 CATTTTCTGC ATTATTGTGT TTTCCGGCTA TATTAATAGG TATTTGTTTG TGTTTTTCTT 4381 TATTTTATGA TTCGAACTCC AATTTGTAAA TTTTCGAACA TATTTCCCTA AAGAAAAAAT 4441 ATGATTAATC TGGAAAAATT GGAAAATTAT TTTTCAAATA AAAAACAAAG AAAAAAATGA 4501 AGAAAAACCT ATTAGTTTGG CCATAAAACG CAAAAATGTC GAAAATGACG TCACTCATCT 4561 GCGCGGGAAA TCAAGAATAA TTCGGCCTTT TTTATTTTTT TGGAAAATCG TAAAACATTT 4621 AGAAAAATTT TTTAATAGTT ATATGGGACT TATTCTGCAT TTAGGGCAAA AGCCAGAGAC 4681 TACTCCACCG TTAACATGAA TTATGAATAT TATTGCGACA AGACCCAAAC ATTGATAAAC 4741 CCAAATCTAG CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 4801 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 4861 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 4921 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 4981 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 5041 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 5101 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5161 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 5221 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 5281 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 5341 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 5401 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5461 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 5521 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 5581 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 5641 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 5701 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 5761 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 5821 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 5881 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 5941 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 6001 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 6061 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 6121 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6181 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6241 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6301 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6361 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 6421 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 6481 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 6541 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 6601 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 6661 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 6721 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 6781 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 6841 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 6901 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 6961 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 7021 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 7081 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 7141 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 7201 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1432 LOCUS ADDGENE_1432 11791 bp DNA circular SYN 01/03/2005 DEFINITION pPD10_46 complete plasmid sequence KEYWORDS . SOURCE pPD10_46 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1432 at www.addgene.org FEATURES Location/Qualifiers source 1..11791 /organism="Addgene plasmid pPD10_46" /mol_type="other DNA" rep_origin complement(170..789) /label="pBR322_origin" CDS complement(944..1804) /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene complement(944..1804) /label="Ampicillin" /gene="Ampicillin" promoter complement(1846..1874) /label="Amp_promoter" rep_origin complement(2846..3152) /label="f1_origin" misc_feature 3657..3673 /label="M13f20_primer" misc_feature complement(3528..3671) /label="lacZ_a" misc_feature 5398..5428 /label="unc54.enh" CDS complement(6293..6973) /label="Orf1" /translation="MKLNNEDGKTKFHVKRALRSDTGTYIIKAENENGTDTAEVKVTV LDHPSSPRGPLDVTNIVKDGCDLAWKEPEDDGGAEISHYVIEKQDAATGRWTACGESK DTNFHVDDLTQGHEYKFRVKAVNRHGDSDPLEAREAIIAKDPFDRADKPGTPEIVDWD KDHADLKWTPPADDGGAPIEGYLVEMRTPSGDWVPAVTVGAGGALDGSQSRFGRRCGS NALLELDG" CDS 9057..9869 /label="Orf2" /translation="MNNELESGSDLDSCIRKIFTMNSWSKVSWLRNNTIRRIRRISSS SLVTSRNSELIFFSLLKIWNSCCFLISWNNESFFPSSILILLFNDESFNWCTTIVWSI PCKINMILIPVHQFRLVNFSWNLVWVLGEDCGVGCEWIGFSLLVHSSDTEFVFVSGLK IWNCGLCCFTSSTWDPSSSCQIHLFDFIMINWSTSVIFWYFPLQICAIFVDIRHFQRS QWARWFSEHNDLNGMFVASRSVGSSKSVQTSMLASCSTEHKSGLVVFAFNLK" misc_feature complement(11570..11588) /label="M13r_primer" promoter complement(11623..11652) /label="lac_promoter" misc_feature complement(11719..11745) /label="lacI.C" ORIGIN 1 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 61 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 121 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 181 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 241 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 301 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 361 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 421 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 481 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 541 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 601 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 661 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 721 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 781 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 841 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 901 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 961 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 1021 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 1081 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 1141 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 1201 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 1261 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 1321 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 1381 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 1441 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 1501 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 1561 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 1621 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 1681 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 1741 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 1801 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 1861 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 1921 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA 1981 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 2041 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 2101 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 2161 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 2221 GGAGAAAATA CCGCATCAGG AANTTCCAAC ATCCAATAAA TCATACAGGC AAGGCAAAGA 2281 ATTAGCAAAA TTAAGCAATA AAGCCTCAGA GCATAAAGCT AAATCGGTTG TACCAAAAAC 2341 ATTATGACCC TGTAATACTT TTGCGGGAGA AGCCTTTATT TCAACGCAAG GATAAAAATT 2401 TTTAGAACCC TCATATATTT TAAATGCAAT GCCTGAGTAA TGTGTAGGTA AAGATTCAAA 2461 CGGGTGAGAA AGGCCGGAGA CAGTCAAATC ACCATCAATA TGATATTCAA CCGTTCTAGC 2521 TGATAAATTC ATGCCGGAGA GGGTAGCTAT TTTTGAGAGG TCTCTACAAA GGCTATCAGG 2581 TCATTGCCTG AGAGTCTGGA GCAAACAAGA GAATCGATGA ACGGTAATCG TAAAACTAGC 2641 ATGTCAATCA TATGTACCCC GGTTGATAAT CAGAAAAGCC CCAAAAACAG GAAGATTGTA 2701 TAAGCAAATA TTTAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT 2761 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA 2821 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG 2881 AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT 2941 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC 3001 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG 3061 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG 3121 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTA CTATGGTTGC 3181 TTTGACGAGC ACGTATAACG TGCTTTCCTC GTTAGAATCA GAGCGGGAGC TAAACAGGAG 3241 GCCGATTAAA GGGATTTTAG ACAGGAACGG TACGCCAGAA TCCTGAGAAG TGTTTTTATA 3301 ATCAGTGAGG CCACCGAGTA AAAGAGTCTG TCCATCACGC AAATTAACCG TTGTCGCAAT 3361 ACTTCTTTGA TTAGTAATAA CATCACTTGC CTGAGTAGAA GAACTCAAAC TATCGGCCTT 3421 GCTGGTAATA TCCAGAACAA TATTACCGCC AGCCATTGCA ACAGGAAAAA CGCTCATGGA 3481 AATACCTACA TTTTGACGCT CAATCGTCTG GAANTTCCAT TCGCCATTCA GGCTGCGCAA 3541 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG 3601 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA 3661 AACGACGGCC AGTGCCAAGC TTGGCTGCAG TGAGTATTTT CGGTTTTTCA CTGGTCTCTG 3721 TAAAAAGAGT AATGCAAAGG GCAAGTTAAC TAGGTCGTAA ATGTATTGAT TTGCTTAAAA 3781 TCTGAAGATC TAGTGGTGAA CCGTGGAAGA TTATCAAGAG GAGGCTGAAG ATCTGTTTAA 3841 GAACCATTAA TCAAACTGGT ATTCTATTTT CACTGGTTGT ATGTAAACAT TCTATCTTAT 3901 TCCTTTTATC ACTGTTCTGC ACTTTCCTAT AAAAAAAGTT GACCGACCGT ACTCTCTGAA 3961 TTCATTTTTC CCGATCTTAC CAACTCCCGA TCTATCTCTA TCCCTGGTTT TTTCTTCGTG 4021 CTCCAATGGA ATTCTTGAGA CTTCCACTAT CTTCTCTGGC ACCCTCCACT ACGCGTAGGC 4081 GTCTCTCGCT TCGTGTATTC CCGGGAAGCC GGGTTCCCGT CTCTCCCGCC GCTGCCGCTG 4141 CCGCACACAG CTTTACACCT CGTAGAATCC CCAAAGAGGG GCGTGGCTTG CGGGTGCCAA 4201 CATCCTCCTG CCGAGGAAGA AGCAGGCACT CATCACTCGC ATCATCAACC TCGACTAAGG 4261 GAGCACCACA TTAGTTTTGA GGTAGAGAAA CCATTTGAAA GAAGCGAGAA ATCATGGAGC 4321 ACGAGAAGGA CCCAGGATGG CAATATCTCC GCCGTACCAG AGAGCAGGTT TTGGAGGTGA 4381 GTATTTATTT TTTATTTATT TATTTTTCTT TCATTTGTCT TTATTGTTAT GCTCATTTCT 4441 ATAGAGTAAA TCGTATAGTA CTCCATAAGT ATCTAATTTT AACAAAACTG AGCATTTATT 4501 TTTGAAATTC TAGATTTTTA ACATACAACT GAGTTAAAGT CTGCCATTTT ACACTTATAG 4561 ATTTTTTAAA TTCAGGTAGA AGATTTGAGC TTGCCTTGTA ATCCTTGTAA TCAATGTTTA 4621 TTTCTGTTTT CTAATTTTTC ATAGATTTAG CTTCAAGATC ATCTTTCCCT TGAAATCATT 4681 GAAATCTTCC TTTCTTAAAT TTTTGCATTG CACTTGGAAT TTTGTGACAC ATTGTGCTGC 4741 AGTAGTACTG TATTTTGAGA CGGCACATTT ATTTTGACTA CACCACAAGC TTAACCTTGA 4801 TTTTACTTTT TGATTCCATT CCATTCATGG GTCTAAAGTG TGAAGCGGTT TAGACCATTT 4861 TTCAACACAA GTTGGTATTC TTTAGAAGTG ATATATTTCT GAATTCTGAT CTCTTTTGTA 4921 CCATTATTGT AATTTCAGGA TCAATCTAAG CCTTATGACT CCAAGAAGAA CGTCTGGATC 4981 CCAGATCCAG AAGAGGGATA CCTTGCCGGA GAAATCACCG CCACCAAGGG AGACCAGGTC 5041 ACCATCGTCA CCGCTCGCGA AATGAGGTTC GTTTTTAAGC TAATGATTAC GGTTTTCAAT 5101 GTAGTGTGAT TCAGGTCACC CTCAAGAAGG AGTTGGTTCA AGAAATGAAT CCACCAAAGT 5161 TCGAGAAGAC CGAAGACATG TCCAACTTGT CTTTCCTCAA CGACGCCTCT GTCCTCCATA 5221 ACTTGCGTTC CCGTTACGCT GCTATGCTCA TCTACGTAAG TTTCATTGAA ACTTAAAGTT 5281 AAGCATTGAG AATCATTCAT ATTCTTCTTC TAAATTCCCA TAAAATCCCG AAACTCCTTC 5341 CCTCTATCTT CTTTTTCTTC TCGTTTTCAA ATGTTTCTCT CTATCCCATT CTCTCATCAA 5401 TTGAGTGGGA TGAGGCTATC TCTGCCTCTC TTCTGAATCT CTGAACCATC TTACATTACA 5461 CTGTGGATGA CGAGCCCCAC AGGCTCCCTT GCATCAGATA CTGCCATTGG GGATGGCAAA 5521 GAAGAGAGAA GGTATTGTGA GGATATATTT TTCTAAGAAA AAACGTTTGA AGAAAAGAAG 5581 ATGAAGAAGA TCTGCTTGAT TCATTGCACA AGTTAGAAGT AACAGGGGTC TATATTTCGA 5641 AGAACTTAAA GGGAATGCAA CTGAACATAA AATTAAACAA AGGGATTGAA TCCTGCAGTA 5701 TTTAAAAATC GAAACTAGAA TGTTTCCTAT TTCAGACCTA CTCCGGACTT TTCTGCGTTG 5761 TCATCAACCC ATACAAGCGT CTCCCAATCT ACACCGACTC TTGCGCCCGT ATGTTCATGG 5821 GAAAGAGAAA GACAGAAATG CCACCACATT TGTTCGCTGT CTCTGACGAA GCCTACCGTA 5881 ACATGCTCCA AGACCACGAG AACCAGTCTA TGCTCATTAC CGGAGAATCT GGAGCCGGAA 5941 AGACTGAGAA CACCAAGAAG GTTATTTGCT ACTTCGCCGC TGTCGGTGCC TCCCAACAGG 6001 AAGGTGGAGC TGAAGTTGAT CCAAATAAGA AGAAGGTCAC CCTTGAGGAC CAAATCGTCC 6061 AGACCAATCC AGTACTTGAA GCCTTCGGTA ACGCCAAGAC TGTCCGTAAC AACAACTCAT 6121 CCCGTTTCGG AAAGTTCATC CGTATCCACT TCAACAAGCA CGGACGCCTC GCATCTTGCG 6181 ATATCGAGCA TTGTGAGTTA CTTTTTGATT TATATTCTAG CATAGCTCAA TAAAAATTAT 6241 GCAACTATTG TTTAAACTAT AAATTTTGCA GACTTGCTCG AGAAATCTCG TGTCATCCGT 6301 CAAGCTCCAG GAGAGCGTTG CTACCACATC TTCTACCAAA TCTACTCTGA CTTCCGTCCA 6361 GAGCTCCACC GGCTCCAACC GTGACAGCTG GTACCCAGTC TCCTGATGGA GTTCTCATTT 6421 CAACGAGATA TCCTTCGATT GGAGCACCTC CATCATCAGC TGGTGGAGTC CACTTGAGAT 6481 CTGCATGATC CTTGTCCCAG TCCACAATTT CTGGAGTTCC TGGCTTATCA GCACGATCGA 6541 ATGGATCCTT GGCGATAATA GCTTCACGAG CCTCCAATGG ATCCGAATCT CCATGTCTGT 6601 TAACAGCCTT GACACGGAAT TTATATTCAT GCCCTTGAGT CAAATCGTCA ACGTGGAAGT 6661 TGGTATCCTT GCTCTCTCCG CAAGCAGTCC ATCTGCCAGT GGCAGCATCT TGCTTTTCAA 6721 TGACATAGTG ACTGATTTCA GCTCCTCCAT CATCTTCTGG TTCCTTCCAT GCAAGATCAC 6781 ATCCATCCTT GACAATATTA GTGACATCGA GAGGTCCACG TGGGCTTGAT GGATGATCAA 6841 GAACAGTAAC CTTCACTTCA GCAGTGTCAG TTCCATTCTC GTTCTCTGCC TTGATGATAT 6901 AGGTTCCTGT ATCCGAACGC AAAGCTCTCT TCACATGGAA TTTAGTCTTG CCGTCTTCAT 6961 TGTTCAACTT CATACGATCA TCAGATTCGA CTGGTGTTCC TTCGAAAGTC CAAGTAATTG 7021 TTGGAGTTGG TTCACCACTG ACTGGAATGT TCAATGAGAA GTCTTGTCCA GCCTTGACCT 7081 TGATTTCTTG AATCGAGTTA CGATCGATGA CTGGTGGAAC TATAATTTAA TTCAATGATT 7141 ATTAGTAATT GATTTAGACT CTTACCATTT CTAGCCTTTG CAACAGCTGA TGCTGAATCA 7201 GATGGATCTC CCAATCCTGC CTTGTTCTTG GCACGGATTC TGAATTCGTA CTTTGATCCT 7261 TCCTTGAGAT TTCCAACAGT AGCATTCGTT TGTCCAGCTG GAACATGAGC AACGTCATTC 7321 CAGAATGGCG AGAACTCGTC CTTCATCTCA ACAACGTATT CCTCGATTGG AGCACCACCG 7381 TCGTTTGCTG GTGGCTTCCA TTCAAGGTCA ACATGATCCT TATCCCAATC AGTAATTTCA 7441 GGAGCATTTG TCTTTCCTGG CTTGTCAAAT GGATCTTTGG CAAGTGTGGT TCCGAAGGTC 7501 TCCAATGGAT CGGACTCTCC TTCAGCATTG ACGGCAGCGA CACGGAACTG AAAATCAAAA 7561 TGTTGTAGGC AATTGAGTTC AAGATTAAAA AATTCTCACT TTATATTCAT GTCCAGGAAT 7621 AAGACCGTCA ACAACAGCTG TAGTCTTATC TCCAGCGACC TTTGCAGCTG GAACCCATCT 7681 TCCACTTGCA GTATCGTACT TTTCGATCAC ATAGTTTTCA ATTGGAATAC CTCCATCATC 7741 ATCTGGTGCA CGCCAATTCA AAGTGACATG ATCACCATGA ACATCGGAAA CATCTAATGG 7801 ACCATTTGGA GAAGTTGGCT TGTCTGAAAA TTTAAAATAT AACCAAATTA ATTGAAGAAA 7861 ACTAATGCTC ACCAATAACA TTGATCTTAA CAGTTGCTTC ATCTTCTCCA TTTGCATTGA 7921 CAGCTTTGAT AGTGAAAGTT CCACTGTCTC CACGTTCCAT TTGCTTCACA ACCAGCTTTG 7981 ATTGGTATTC TGGGTTATCA AGCTTGACGC GTCCTCCATT TCTGATTTCC CTGTTGTCTG 8041 GCCAATGCCA TGTGACTGTT GGTGCAGGTT CTCCTGATAC TGGAACATCG AAGGAAATTG 8101 ATTGACCAGC CTTGATAGTC AGATTCTTGA GAGCATCTCT GTCGATGTGT GGCTTCACTG 8161 AAAATTAAAA AATAGCACTT TTCCTCAAAA TTAAACAATC AACTCACAGT TTCTTGGTTT 8221 AGCAATAACA GCCTTGCTAG CATCAGATGG ATCAGATGGG CCTCCCTTGT TAACAGCAAC 8281 AACACGGAAT TCGTATTCCT CATTTGGTGT GAGGTCTGGA ACGGTTGCAG TTGTCTGACC 8341 GCCAGGAACA GTGATGGCTG GTTCCCATCT GCAAAATTAG AATTGGCAAA ACTTCAAATT 8401 AAAAAAAAAC GATACCTTCC GTATTTGGTT CTCTTCTCAA TTTGGTACTC CTCAATTGGA 8461 GCGCCGCCAT CAGAAAGTGG TGGATCCCAC TTGAGATCAA CATGATCAGA ATCCCAATCA 8521 GTTGGCTCTG GACGACCTGG TCTATCTGGA CGATCAAATG GATTTTTAGC CAAAATATCG 8581 GTTTCAGTTT CCAATGGATC AGAAGGTCCT TCGCTGTTGA CAGCCTTGAC ACGGAACTTG 8641 TAATGATCTC CCTTGTTGAG TGAATCGACT GTGAAGTGGG TATCTCCACT ACGTCCACAT 8701 GGAACCCAGA TTCCGTCCTT GGTGTTCATC TTTTCAATTT CATAGAACTC AATTGGCTCT 8761 CCTCCGTCAT CCTCTGGTGG TTTCCAGTCA AGAGTTGCAC GATCTTCGAA GACATCAGTT 8821 ACCTCGATTG GTCCCTTTGG TTTCGTTGGC TTGCTTTTTA CCTTGATATT GATAGTGACA 8881 CTGTCAGTTC CATTAATGTT GGTTGCAGTG ATGGTATATT TTCCACTTTG CTTACGAAGT 8941 GCCTTTGGCA AAGCAAATCT CGAGATGTAT GGCTCATTTT CAATTTGAGC CTTGCTCTCT 9001 CCGATTCCTT TTCCATTGAA TGACCATGTT ACATCTGGTG CTGGCTCACC ATCAATATGA 9061 ACAATGAACT TGAGAGTGGC TCCGACCTTG ACAGTTGTAT CAGAAAGATC TTCACGATGA 9121 ATTCTTGGAG CAAGGTTTCT TGGCTTCGCA ACAACACGAT CAGAAGGATC AGAAGGATCT 9181 CCAGTTCCAG CCTTGTTACG AGCAGAAATT CTGAATTGAT ATTCTTCTCC CTCCTTAAGA 9241 TTTGGAACAG TTGCTGTTTT CTGATCTCCT GGAACAACGA GAGCTTCTTC CCATCGTCCA 9301 TTCTTATCCT TCTTTTCAAT GACGAAAGCT TCAATTGGTG CACCACCATC GTCTGGAGCA 9361 TTCCATGCAA GATCAACATG ATCCTTATCC CAGTCCACCA ATTCCGGCTT GTCAACTTTT 9421 CCTGGAACCT CGTATGGGTT CTTGGCGAGG ATTGCGGTGT TGGTTGTGAG TGGATCGGAT 9481 TCTCCCTCCT TGTTCACAGC TCTGACACGG AATTTGTATT CGTGTCCGGG TTGAAGATTT 9541 GGAACTGTGG TCTTTGTTGT TTCACTTCTT CCACATGGGA CCCATCTTCC AGTTGCCAAA 9601 TCCATCTTTT CGATTTCATA ATGATCAATT GGAGCACCTC CGTCATCTTC TGGTACTTTC 9661 CACTCCAAAT CTGCGCGATC TTCGTAGACA TTCGACACTT CCAAAGGTCC CAATGGGCTA 9721 GATGGTTTTC CGAGCACAAT GACCTCAACG GAATGTTTGT CGCTTCCAGA AGCGTTGGTA 9781 GCAGTAAGAG TGTACAAACC AGCATGCTTG CGAGTTGCTC CACGGAGCAC AAATCGGGTC 9841 TTGTAGTCTT CGCTTTCAAT CTGAAATAAT CGTAAATTAG AATGTTCAAA AATATATAAA 9901 ATCTTACCCT GATCTTTTGA TCATCGACTG GTTTCTCATT GAAGATCCAT TCCTTCTTCG 9961 GTGGTGGTTC TCCGCGTACT GGAACATCGA ATTCCACATC GTTTCCTACC TTGATAGTGA 10021 TCGTCTTCAT TGCTTCACGA TCAATCCATG GCTTCAAGTT TCTTGGCTTT GCAACAACCT 10081 TTCGGCTTGG GTCTGAAGCC TCTCCCGGTC CGGCCTTGTT AACAGCCTTC ACACGGAACT 10141 GGTACTCTTC TCCCTCCTTG AGTCCAAGAA TCTCAGCATT GGTTTGATCT CCAGAAACCT 10201 TTCCGCATTC TTGCCAGTCT CTTCCATGTT TGCCCTTCTT CTCAATGACG TATTGAGTGA 10261 TTGGAGCTCC GCATCGGCCG CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT 10321 CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT 10381 ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC 10441 ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA 10501 AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT 10561 CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA 10621 ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT 10681 CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG 10741 TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT 10801 TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC 10861 ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG 10921 TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT 10981 TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTAAAATTA AACATTTTTC 11041 TAAATTTTAT ATGATTTCTT TTAAATTTGC AAAAATTACT TAAATTTGAA TTCCCGCGCA 11101 AATGAGTGAC TTCATTTTCT GCATTATTGT GTTTTCCGGC TATATTAATA GGTATTTGTT 11161 TGTGTTTTTC TTTATTTTAT GATTCGAACT CCAATTTGTA AATTTTCGAA CATATTTCCC 11221 TAAAGAAAAA ATATGATTAA TCTGGAAAAA TTGGAAAATT ATTTTTCAAA TAAAAAACAA 11281 AGAAAAAAAT GAAGAAAAAC CTATTAGTTT GGCCATAAAA CGCAAAAATG TCGAAAATGA 11341 CGTCACTCAT CTGCGCGGGA AATCAAGAAT AATTCGGCCT TTTTTATTTT TTTGGAAAAT 11401 CGTAAAACAT TTAGAAAAAT TTTTTAATAG TTATAGTGGG ACTGTATTCT GTCATTTAGG 11461 GCAAAAGCCA GAGACGCTAC TCCACCGTTA ACATGAATTA TGAATATTAT TGCGACAAGA 11521 CCCAAACATT GATAAACCGC AAATCTAGAG ATCCCCGGGA ATTCGTAATC ATGGTCATAG 11581 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 11641 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 11701 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 11761 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG C // 1433 LOCUS ADDGENE_1433 5730 bp DNA circular SYN 01/03/2005 DEFINITION pPD16_43 complete plasmid sequence KEYWORDS . SOURCE pPD16_43 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1433 at www.addgene.org FEATURES Location/Qualifiers source 1..5730 /organism="Addgene plasmid pPD16_43" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 3644..3672 /label="Amp_promoter" gene 3714..4574 /label="Ampicillin" /gene="Ampicillin" CDS 3714..4574 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4729..5348 /label="pBR322_origin" misc_feature 5564..5590 /label="lacI.C" promoter 5657..5686 /label="lac_promoter" misc_feature 5721..5739 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 3301 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 3361 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 3421 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 3481 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT 3541 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 3601 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 3661 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 3721 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 3781 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 3841 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 3901 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 3961 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 4021 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 4081 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 4141 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 4201 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 4261 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 4321 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 4381 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 4441 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 4501 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 4561 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 4621 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 4681 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 4741 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 4801 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 4861 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC 4921 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 4981 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 5041 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 5101 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG 5161 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 5221 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 5281 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 5341 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 5401 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 5461 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 5521 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA 5581 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC 5641 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA 5701 GCGGATAACA ATTTCACACA GGAAACAGCT // 1434 LOCUS ADDGENE_1434 7003 bp DNA circular SYN 01/03/2005 DEFINITION pPD16_51 complete plasmid sequence KEYWORDS . SOURCE pPD16_51 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1434 at www.addgene.org FEATURES Location/Qualifiers source 1..7003 /organism="Addgene plasmid pPD16_51" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 4917..4945 /label="Amp_promoter" CDS 4987..5847 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4987..5847 /label="Ampicillin" /gene="Ampicillin" rep_origin 6002..6621 /label="pBR322_origin" misc_feature 6837..6863 /label="lacI.C" promoter 6930..6959 /label="lac_promoter" misc_feature 6994..7012 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGGGCCGC TGTCATCAGA TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT 3301 TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT 3361 TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA 3421 TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG 3481 CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT 3541 TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA 3601 AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC 3661 TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA 3721 TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC 3781 CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA 3841 TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG 3901 TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA 3961 ATACATTCCC AATATACCAA ACATAACTGT TTAAAATTAA ACATTTTTCT AAATTTTATA 4021 TGATTTCTTT TAAATTTGCA AAAATTACTT AAATTTGAAT TCCCGCGCAA ATGAGTGACT 4081 TCATTTTCTG CATTATTGTG TTTTCCGGCT ATATTAATAG GTATTTGTTT GTGTTTTTCT 4141 TTATTTTATG ATTCGAACTC CAATTTGTAA ATTTTCGAAC ATATTTCCCT AAAGAAAAAA 4201 TATGATTAAT CTGGAAAAAT TGGAAAATTA TTTTTCAAAT AAAAAACAAA GAAAAAAATG 4261 AAGAAAAACC TATTAGTTTG GCCATAAAAC GCAAAAATGT CGAAAATGAC GTCACTCATC 4321 TGCGCGGGAA ATCAAGAATA ATTCGGCCTT TTTTATTTTT TTGGAAAATC GTAAAACATT 4381 TAGAAAAATT TTTTAATAGT TATAGTGGGA CTGTATTCTG TCATTTAGGG CAAAAGCCAG 4441 AGACGCTACT CCACCGTTAA CATGAATTAT GAATATTATT GCGACAAGAC CCAAACATTG 4501 ATAAACCGCA AATCTAGCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC GCGCGTTTCG 4561 GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT 4621 AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC 4681 GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT 4741 GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC 4801 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT 4861 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA 4921 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG 4981 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC 5041 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG 5101 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 5161 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA 5221 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT 5281 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA 5341 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA 5401 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 5461 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC 5521 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT 5581 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT 5641 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT 5701 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 5761 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA 5821 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG 5881 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT 5941 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA 6001 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA 6061 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT 6121 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG 6181 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC 6241 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA 6301 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC 6361 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC 6421 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA 6481 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 6541 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA 6601 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT 6661 CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG 6721 TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA 6781 GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC 6841 AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG 6901 AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG 6961 TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCT // 1435 LOCUS ADDGENE_1435 7081 bp DNA circular SYN 01/03/2005 DEFINITION pPD18_32 complete plasmid sequence KEYWORDS . SOURCE pPD18_32 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1435 at www.addgene.org FEATURES Location/Qualifiers source 1..7081 /organism="Addgene plasmid pPD18_32" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" promoter 4995..5023 /label="Amp_promoter" CDS 5065..5925 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5065..5925 /label="Ampicillin" /gene="Ampicillin" rep_origin 6080..6699 /label="pBR322_origin" misc_feature 6915..6941 /label="lacI.C" promoter 7008..7037 /label="lac_promoter" misc_feature 7072..7090 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGATC GCCATCTCGC GCCCGTGCCT 3361 CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC 3421 ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT 3481 TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA 3541 AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA 3601 CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT 3661 AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT 3721 TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT 3781 TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT 3841 TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC 3901 TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA 3961 GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT 4021 TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT AAAATTAAAC 4081 ATTTTTCTAA ATTTTATATG ATTTCTTTTA AATTTGCAAA AATTACTTAA ATTTGAATTC 4141 CCGCGCAAAT GAGTGACTTC ATTTTCTGCA TTATTGTGTT TTCCGGCTAT ATTAATAGGT 4201 ATTTGTTTGT GTTTTTCTTT ATTTTATGAT TCGAACTCCA ATTTGTAAAT TTTCGAACAT 4261 ATTTCCCTAA AGAAAAAATA TGATTAATCT GGAAAAATTG GAAAATTATT TTTCAAATAA 4321 AAAACAAAGA AAAAAATGAA GAAAAACCTA TTAGTTTGGC CATAAAACGC AAAAATGTCG 4381 AAAATGACGT CACTCATCTG CGCGGGAAAT CAAGAATAAT TCGGCCTTTT TTATTTTTTT 4441 GGAAAATCGT AAAACATTTA GAAAAATTTT TTAATAGTTA TAGTGGGACT GTATTCTGTC 4501 ATTTAGGGCA AAAGCCAGAG ACGCTACTCC ACCGTTAACA TGAATTATGA ATATTATTGC 4561 GACAAGACCC AAACATTGAT AAACCGCAAA TCTAGCCTAC TAGTCGGCCG TACGGGCCCT 4621 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 4681 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 4741 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 4801 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 4861 GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 4921 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 4981 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 5041 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 5101 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 5161 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 5221 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 5281 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 5341 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 5401 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 5461 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 5521 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 5581 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 5641 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 5701 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 5761 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 5821 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 5881 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 5941 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 6001 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 6061 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 6121 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 6181 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 6241 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 6301 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 6361 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 6421 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 6481 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 6541 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 6601 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 6661 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 6721 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 6781 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 6841 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 6901 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 6961 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 7021 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 7081 T // 1436 LOCUS ADDGENE_1436 10734 bp DNA circular SYN 01/03/2005 DEFINITION pPD18_49 complete plasmid sequence KEYWORDS . SOURCE pPD18_49 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1436 at www.addgene.org FEATURES Location/Qualifiers source 1..10734 /organism="Addgene plasmid pPD18_49" /mol_type="other DNA" misc_feature 35..58 /label="myo3.-2341" misc_feature 2354..2377 /label="myo3.Tsa" misc_feature 2420..2443 /label="myo3.N" CDS 3726..6971 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MNPPKFDKTEDMANLTFLNEGVPSSEKMTAPKKKRKVPVGEDQK QHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTN VTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVG YGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKP TTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGG EIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFRE VRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHY PNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNH PSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDE DQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGF VWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQ QFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIEL PELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHL TTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEA TRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTY RIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAAC FDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSH RHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 3807..3833 /label="SV40.NLS" gene 3924..6968 /label="lacZ" /gene="lacZ" promoter 8648..8676 /label="Amp_promoter" CDS 8718..9578 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 8718..9578 /label="Ampicillin" /gene="Ampicillin" rep_origin 9733..10352 /label="pBR322_origin" misc_feature 10568..10594 /label="lacI.C" promoter 10661..10690 /label="lac_promoter" misc_feature 10725..10743 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCAAGC TTGGGCTGCA GGTCGGCTAT AATAAGTTCT TGAATAAAAT 61 AATTTTCCCG ACAAAACATG AGTATTTCTT TCGAAAATAA AAGTGCAGGC TAATTAGAGA 121 TTATTCTGTA ATTAACTGCA TAATTTGTCA CGTGCCATAG TTTTACATTC CACTACGTCA 181 TAGTTCTTAA AATACTAATC TCCTGAAAAT AGAAGTAGGT GAAGAAAGTT TAATTATCAG 241 TTCTAAAATG ACAATTGATC TTTGGAATAT GTTCTGAAAC TACCGATCAT TGAACAGATG 301 CTATTTGAAT GATATAGAAT TGTATATTTG CAATTTCTGA AACGCGTTCT TAAAGGCACA 361 CAGATTAATT CAAAAGGGTC TGGCCGCAAA AAGGTTTATG GTGGCCGATT TTGAGTTTTG 421 TGTGTGATTG CTTTTTCACA ATCAGTGTTT TCAGGATTAT GTGATGAACT AGATCTTCAA 481 GTTTCGTTAC ATTTCATATG TTTTCGGAAC TCACGAAGTA CATATTGGGT ATTGTGCTCA 541 AAAAATTCAG CAATCAGCTT CGCTCCGCTG ACTTTAGAAC CCAAAAAAAT AGTATGGCCA 601 AACTGACTGT GTTACGATCA TTTCAATTTT TCAATACATA TTTAAGATTT CTAAGAGTAA 661 GAAGGTCAAA AACTGTTCTG GAATACATAT ATATTTTTCA GGTTACAATT AGTCAAAAAG 721 TGCACTGAAA TATACGTTTT AATTTCACGA ATAACCCAAT TAGTTCAATG TATTTTTGGT 781 CAACCAACGT TAAAGTTTGG CTTCCAACCA ATTATCATTT CTGATCAACC ACAATGTTTT 841 TTCTTTATCT GCAAGTTAAT TTTTTATTTT TATCCAGATG TTTGGCATAT TTTTCAATTC 901 TTCACTAGCG CCCACTTCTT GCACTTCCGG CGCCCTGAAT CTAATGCATC TGTTGCAAGA 961 ATTGAAAGAC CAATCAACAC ATTGTTTTCT TCACGAGATA CTGAAGAAAA TGAATAAAAA 1021 CAGAGAAAAA GAGCCATGTG ATTAGTGACA ACTGTTGCTA ACAGATACCA TAGCTTGGAC 1081 TTGGTACGTG ATGGCAACGT ATGGGTCAAC AAAAATGATT GCAGAGGGGG TGCAAAACAG 1141 TCAAGTCGAG AAAATATGAA AAACAGAAAA CAAAGAACAG AAAAATGGGT TTGAGAGTCA 1201 GTATAATTTA TAAAAGAAAA ATTGTACATA GAAATTAACC ATTTTTGTAG AAGAAGTTAT 1261 TTTTCAAGCA TCGTTAAAAA TTATTCAAAG CACCTTATTT CATATTTAAT TTTAAACATG 1321 GTTAAATGAA CAACACGGTG CGCAATCAGG AAAACTTGAA ATCTGAAACT GTTGTTGTGA 1381 TCTTCTTCGC AACTGTTCAG ATAGCACTAG TGTAATGTTA AGAGTGCGCG AATATAATGG 1441 AATATAATGG ATCACACCTC CTGCCATCAG GTAAACGTCT CTGTTATCAC ATATTTCCAA 1501 CTATTAAATT TTTACCTTTT ACAGTTTTAC ATTTTTTTGA AAAAAGTAAC TTTTTGTCTT 1561 CAAAATCCCT GACGAAAATA TCAAATATTT TAATCGAGAC TGCAGAGGAA CCGATTGATG 1621 ATTTGGAAAA TCCAGCTTTA CCTGTGTAAG AACTGAAAAG TTTCATAACC CTAGGGTATT 1681 CCCAGTTACA TTCCCCACTG GCTAACAATA GCACCCAGTT TTTCATCACC TTTCTTCAAA 1741 TTTCTCGGCG ATTTGTTAAA AACAAAATTT GTGTCCCTTC TCTGATATCT CTATGTCTCT 1801 AAACACAAGT TCATCGGAAA ACGAAGGAGG GTAGGTGTTG GTTGGGCTCC CGAAGTGAAA 1861 ATAGAAGAGC AAGAATAGAA TATTAGAGAG AGAGTGCAGA GAGGGCGGGA TAGCTCCCGG 1921 GATTCCGTTT TCTTCTTCTT TATCTTCAAC GATGATGTGT GTGCGTGTTG TATAGATTCT 1981 GTTGCTCCCC CACAACTCGC TCCGAAGGCT CAATACAATT CAATTGATAT TGGAGGAGAG 2041 CCTACCGGAG TGGGAGGATA AGAAGAAACA TAAGAAGAAG AAGAAGAAGA AGCATGCTTC 2101 TGGTTTTTGA TGCTATGAAA ACGGCACAAA AAGATGATTG AGGTCCCTTT TCAATACCTT 2161 CTCTCATCTT TCAAATCCCA TTGAAACCTA AAACTTCTCA CCACGCTTTA CCATTGTTCT 2221 CCAAAAACTT ATAGCAATGT CTATAACTTT TTTATCTCTG AAAAGCAGTG TTCCATTTTT 2281 CTTTTTCCTA TTTTATTTCA ATTGTTTCTC ACATTTCGTT TGGATTCTTT GCTTGTCAAC 2341 CAGCTTCTTC TTCCACTTTT ACCGTCTAAT TTTCAGGGCA GGGAGCCATC AAACCCACGA 2401 CCACTAGATC CATCTAGAAA TGTCTGGAAA TCCAGACGCA TTCGAAAATG ATCCAGGATT 2461 CCCATTCTTG GGAATCTCGA GAGAGGCCAG AGCTGCCACT GCTGCTCGTC CTTTTGATTC 2521 CAAGAAGAAC TGCTGGATTC CGGATCCAGA AGATGGTAAG TCTTGATTTC GAATTGAAAA 2581 TTTTAAAGAA AAATTTTGAA CCACGTGCTG TAATATTTGG CAAGAATATT TTCCCATAAT 2641 TTTAATAGTA ACTGTGATAT GTTCTAATTT TAGAAATCAG TTGTCAACGC TAAAAATGTA 2701 AGGGTTACAA TAGTGGTATT GCAAAGAGTT ATGATGGTTA AGTTTGTAAA TACTTTTTTT 2761 CTGTTAATGA AACTAATTTG GATACAAATT TTTCATTTGA TCTAAACAGA ACATCTTAAT 2821 CAAAAAACAC TTTAAAACTC TTGCTTTGAT ATTGTTTGTT CAATTTCCTA ATTCTTACCA 2881 AACCAATTCA AGCTAACTTT CATTATGTCC ATTCCATTAA CAAAAACTAT TATTACACCT 2941 TCTCTCTCAT CGCCAGTGTC CACATTCATC CTACTCTCCT ACGCTTTCAT CTGTGTCTCT 3001 CAGATCTGCA TTTTATCTAT GATACATCAC ACTTTCCACC GACCCCGCCG GGCAACCAGT 3061 GGTGGCGGAT TTTTGAACAT CTGAGGTTCT CTCATCTCTC GGTCATCTGA TGAAGCACAT 3121 ACACACATAC ACACAACGGC ACTTTGTAAT CTTTCCGGAG AAGCGGGCAG GCACACACGT 3181 ACGCTATGAA GCCGGAGCAA GAGCTTCTCC TGCCGTTTGA ACTGCGGCTT CTGCTGTTGG 3241 CAGGAAAGAT GATGAGATTT CTTGGAATTT AAGATTGAGA TATTTGACGT CCCACATTTT 3301 ATGGATGGAC AACAATATGT ACATGTTTTG AAGATTTAAT ATTGAAATGT GGATATGAAA 3361 TCGGAATTTA AAAAGTCAAG GTCAATAAAA TGGTAATTCT GACCAGATCT ATTATTCGAG 3421 GATGACAAAT AAAACTAGGA TTAAATCAAA AATACTAAAT TTGAAAATGA AAAAGAAAAG 3481 ATTGTTCTAT AACTTATAAA TCTCACAATA ATGATTTTTG TACAGAAACA AAAACTAAAT 3541 GACATAATCG AATAAAACAT TCTGATTTTA ATCAGCCGCA TGCGACAAAA CATATATTTC 3601 TTGGAGGCTA ATATTAAATT TCAGGATTCG TCGCCGCCGA GATCCAATCC ACCACCGGCG 3661 AACAGGTGAC CGTGGTAACT GTGAAGGGAA ACCAGATTAC CGTCAAAAAA GATCAATGCC 3721 AAGAGATGAA CCCGCCAAAA TTCGACAAGA CCGAGGATAT GGCTAACTTG ACCTTTTTGA 3781 ATGAGGGGGT ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG 3841 TGGGTGAAGA CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG 3901 CGCTGTATGG CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 3961 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 4021 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG 4081 CCTGGTTTCC GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG 4141 CCGATACTGT CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA 4201 CCAACGTGAC CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG 4261 GTTGTTACTC GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA 4321 TTATTTTTGA TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT 4381 ACGGCCAGGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG 4441 AAAACCGCCT CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG 4501 ATATGTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC 4561 AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG 4621 AGGCTGAAGT TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT 4681 GGCAGGGTGA AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG 4741 AGCGTGGTGG TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT 4801 GGAGCGCCGA AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA 4861 CGCTGATTGA AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC 4921 TGCTGCTGCT GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC 4981 CTCTGCATGG TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG CTGATGAAGC 5041 AGAACAACTT TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG TGGTACACGC 5101 TGTGCGACCG CTACGGCCTG TATGTGGTGG ATGAAGCCAA TATTGAAACC CACGGCATGG 5161 TGCCAATGAA TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC GAACGCGTAA 5221 CGCGAATGGT GCAGCGCGAT CGTAATCACC CGAGTGTGAT CATCTGGTCG CTGGGGAATG 5281 AATCAGGCCA CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT 5341 CCCGCCCGGT GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC 5401 CGATGTACGC GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA 5461 AAAAATGGCT TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA TACGCCCACG 5521 CGATGGGTAA CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT CAGTATCCCC 5581 GTTTACAGGG CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA TATGATGAAA 5641 ACGGCAACCC GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC GATCGCCAGT 5701 TCTGTATGAA CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCGCTG ACGGAAGCAA 5761 AACACCAGCA GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG 5821 AATACCTGTT CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA 5881 AGCCGCTGGC AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG 5941 AACTGCCTGA ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG 6001 TGCAACCGAA CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCCTGG CAGCAGTGGC 6061 GTCTGGCGGA AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA 6121 CCACCAGCGA AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC 6181 AGTCAGGCTT TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC 6241 GCGATCAGTT CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA 6301 TTGACCCTAA CGCCTGGGTC GAACGCTGGA AGGCGGCGGG CCATTACCAG GCCGAAGCAG 6361 CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG ACCGCTCACG 6421 CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG ATTGATGGTA 6481 GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG CATCCGGCGC 6541 GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG CTCGGATTAG 6601 GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC TGGGATCTGC 6661 CATTGTCAGA CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA 6721 CGCGCGAATT GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC 6781 GCTACAGTCA ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG 6841 GCACATGGCT GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC 6901 CGTCAGTATC GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 6961 GTCAAAAATA ATAGGGGCCG CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT 7021 CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT 7081 ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC 7141 ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA 7201 AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT 7261 CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA 7321 ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT 7381 CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG 7441 TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT 7501 TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC 7561 ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG 7621 TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT 7681 TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTAAAATTA AACATTTTTC 7741 TAAATTTTAT ATGATTTCTT TTAAATTTGC AAAAATTACT TAAATTTGAA TTCCCGCGCA 7801 AATGAGTGAC TTCATTTTCT GCATTATTGT GTTTTCCGGC TATATTAATA GGTATTTGTT 7861 TGTGTTTTTC TTTATTTTAT GATTCGAACT CCAATTTGTA AATTTTCGAA CATATTTCCC 7921 TAAAGAAAAA ATATGATTAA TCTGGAAAAA TTGGAAAATT ATTTTTCAAA TAAAAAACAA 7981 AGAAAAAAAT GAAGAAAAAC CTATTAGTTT GGCCATAAAA CGCAAAAATG TCGAAAATGA 8041 CGTCACTCAT CTGCGCGGGA AATCAAGAAT AATTCGGCCT TTTTTATTTT TTTGGAAAAT 8101 CGTAAAACAT TTAGAAAAAT TTTTTAATAG TTATAGTGGG ACTGTATTCT GTCATTTAGG 8161 GCAAAAGCCA GAGACGCTAC TCCACCGTTA ACATGAATTA TGAATATTAT TGCGACAAGA 8221 CCCAAACATT GATAAACCGC AAATCTAGCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT 8281 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC 8341 AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT 8401 TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA 8461 CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC 8521 TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT 8581 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT 8641 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT 8701 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG 8761 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG 8821 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 8881 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 8941 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 9001 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA 9061 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT 9121 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 9181 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 9241 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 9301 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG 9361 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG 9421 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 9481 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 9541 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT 9601 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC 9661 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG 9721 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT 9781 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC 9841 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC 9901 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG 9961 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT 10021 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT 10081 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC 10141 ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 10201 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA 10261 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG 10321 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT 10381 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA 10441 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG 10501 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA 10561 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG 10621 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 10681 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT // 1437 LOCUS ADDGENE_1437 8255 bp DNA circular SYN 01/03/2005 DEFINITION pPD20_97 complete plasmid sequence KEYWORDS . SOURCE pPD20_97 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1437 at www.addgene.org FEATURES Location/Qualifiers source 1..8255 /organism="Addgene plasmid pPD20_97" /mol_type="other DNA" misc_feature 74..98 /label="myo2.-1090" CDS 1049..4498 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMTAPKKK RKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFA SWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQM HGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAF HLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGI FRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQ VASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIE AEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQN NFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERV TRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDI ICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAF RQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPH PALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDV APQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTL PAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAP LDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQG KTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQE NYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRY SQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVW CQK" misc_feature 1162..1185 /label="myo2.5'" misc_feature 1259..1300 /label="SynIVS.A" misc_feature 1334..1360 /label="SV40.NLS" gene 1451..4495 /label="lacZ" /gene="lacZ" promoter 6169..6197 /label="Amp_promoter" CDS 6239..7099 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6239..7099 /label="Ampicillin" /gene="Ampicillin" rep_origin 7254..7873 /label="pBR322_origin" misc_feature 8089..8115 /label="lacI.C" promoter 8182..8211 /label="lac_promoter" misc_feature 8246..8264 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAGCTT 61 GCATGCCTGC AGGTCGAGGC ATTTGAATTG GGGGTGGTGG ACAGTAACTG TCTGTAATAA 121 TAATTACTCC TGACCAGGTT GCAATTCGAG TTTTGATAAG CATAATTATA CCTTGTACAT 181 TGTGGGTTTT GTGCTGTGGA CGTTTTATTG TGACATCCCC ATAAGCTACA AGAACAAAAA 241 TGAAATgTAA AAGTATTGAA AACGTCGTAC ATTTTATATC TGAGTAGTAT CCTTTGCTTT 301 AAATGTCCAT AAAAcTAATT TTATAATCAA TAAAACAACG TTTGTAAATC AACTGAGTTT 361 ACAAGTAGAG ACATTGAGGG ATACTTTCAC TATGCTAAAG TGATAATCGA CCAAATAATA 421 ACTtCACTTT GGTATTTATT CCTGTCTTAT AAaTGTTATG TATGAATTAA ATTCATATGC 481 ATATGGCTCA CTCTGACAAt ttttAATAAT CTTCCAGATC AATATTGACT ACCGATGCGG 541 GTGGTCTTTT GCTTTGAATT CTGCTGAACT TTACACCCCG AACAGCAATG TGTGCTTCAG 601 CcTAAAAAAA AGTAAGTGTG TTAATCAGTg CCCCCGATTC TTCATTTTTT GCCCCTCTCT 661 CCCGTTTCGT CGGCAAAAGA AGAGAAAATA AAGATAAGTC TCAAGATAGG TTGGTAATCG 721 CTAAAGTGGT TGTGTGGATA AGAGTAGCAA AATGGCAGGA AGAGCACTTT GCGCGCACAC 781 ACTGTACTCA TTGTTCTGGA TAAAATTCTC TCGTTGTTTG CCGTCGGATG TCTGCCTCTC 841 TGCATTGAGC CGGCTTCTTC ACTATCTTTA GTTAACCTAA AATGCCGTTT CTTTTCTCGT 901 ATCCCcACTA TcCCGTTGAG GTTCTCTGCT CTCTTCGCTC CCTACCGCCA GCGAGCAACT 961 ATCCGTGGGG GCGCCTTGCT CGGAAGATGG GGGGGAAGAA AGAAGATTTT TGCTATTTGC 1021 ACTTGAGAAA GAGACTTTTC CTGCGTCGAT GGTTAGAGAA CAGTGTGCAG ACACTTTTCA 1081 GCTACCTAGA atTACAatTG GATATCCCCG CCTCCCAATC CACCCACCCA GGGAAAAAGA 1141 AGGGCTCGCC GAAAATCAAA GTTATCTCCA GGCTCGCGCA TCCCACCGAG CGGTTGACTT 1201 CTCTCCACCA CTTTTCATTT TAACCCTCGG GGTACGGGAT TGGCCAAAGG ACCCAAAGGT 1261 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 1321 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTACCGGTGG GTGAAGACCA 1381 GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT TTCAACGCGC TGTATGGCGA 1441 GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA 1501 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA 1561 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC 1621 ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT 1681 CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA 1741 TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT 1801 CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG 1861 CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG GTCGGTTACG GCCAGGACAG 1921 TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC 1981 GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT 2041 GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA TCAGCGATTT 2101 CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT GTACTGGAGG CTGAAGTTCA 2161 GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT TCTTTATGGC AGGGTGAAAC 2221 GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA 2281 TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT 2341 CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC TGATTGAAGC 2401 AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA AATGGTCTGC TGCTGCTGAA 2461 CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG CATCATCCTC TGCATGGTCA 2521 GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG ATGAAGCAGA ACAACTTTAA 2581 CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG TACACGCTGT GCGACCGCTA 2641 CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC GGCATGGTGC CAATGAATCG 2701 TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA CGCGTAACGC GAATGGTGCA 2761 GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG GGGAATGAAT CAGGCCACGG 2821 CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC GATCCTTCCC GCCCGGTGCA 2881 GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT ATTTGCCCGA TGTACGCGCG 2941 CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG TCCATCAAAA AATGGCTTTC 3001 GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC GCCCACGCGA TGGGTAACAG 3061 TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG 3121 CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG 3181 GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG 3241 TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG GAAGCAAAAC ACCAGCAGCA 3301 GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG ACCAGCGAAT ACCTGTTCCG 3361 TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG GATGGTAAGC CGCTGGCAAG 3421 CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT 3481 ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC 3541 GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG CAGTGGCGTC TGGCGGAAAA 3601 CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT 3661 GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT 3721 TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC 3781 CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC 3841 CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG 3901 CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA 3961 GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC 4021 GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA 4081 CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA 4141 CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT 4201 GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC TGCGGGACGC GCGAATTGAA 4261 TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC ATCAGCCGCT ACAGTCAACA 4321 GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG GAAGAAGGCA CATGGCTGAA 4381 TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC TGGAGCCCGT CAGTATCGGC 4441 GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA 4501 GGGGCCGCTG TCATCAGATC GCCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 4561 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 4621 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 4681 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 4741 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 4801 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 4861 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 4921 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 4981 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 5041 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 5101 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 5161 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 5221 ACATTCCCAA TATACCAAAC ATAACTGTTT AAAATTAAAC ATTTTTCTAA ATTTTATATG 5281 ATTTCTTTTA AATTTGCAAA AATTACTTAA ATTTGAATTC CCGCGCAAAT GAGTGACTTC 5341 ATTTTCTGCA TTATTGTGTT TTCCGGCTAT ATTAATAGGT ATTTGTTTGT GTTTTTCTTT 5401 ATTTTATGAT TCGAACTCCA ATTTGTAAAT TTTCGAACAT ATTTCCCTAA AGAAAAAATA 5461 TGATTAATCT GGAAAAATTG GAAAATTATT TTTCAAATAA AAAACAAAGA AAAAAATGAA 5521 GAAAAACCTA TTAGTTTGGC CATAAAACGC AAAAATGTCG AAAATGACGT CACTCATCTG 5581 CGCGGGAAAT CAAGAATAAT TCGGCCTTTT TTATTTTTTT GGAAAATCGT AAAACATTTA 5641 GAAAAATTTT TTAATAGTTA TATGGGACTT ATTCTGCATT TAGGGCAAAA GCCAGAGACT 5701 ACTCCACCGT TAACATGAAT TATGAATATT ATTGCGACAA GACCCAAACA TTGATAAACC 5761 CAAATCTAGC CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC 5821 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 5881 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG 5941 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 6001 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG 6061 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 6121 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 6181 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 6241 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 6301 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 6361 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 6421 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 6481 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 6541 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 6601 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 6661 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 6721 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 6781 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 6841 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 6901 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 6961 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 7021 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 7081 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 7141 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 7201 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 7261 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 7321 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 7381 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 7441 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 7501 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 7561 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 7621 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT 7681 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 7741 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 7801 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 7861 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 7921 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 7981 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 8041 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 8101 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 8161 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 8221 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCT // 1438 LOCUS ADDGENE_1438 6556 bp DNA circular SYN 01/03/2005 DEFINITION pPD21_28 complete plasmid sequence KEYWORDS . SOURCE pPD21_28 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1438 at www.addgene.org FEATURES Location/Qualifiers source 1..6556 /organism="Addgene plasmid pPD21_28" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" promoter 4470..4498 /label="Amp_promoter" gene 4540..5400 /label="Ampicillin" /gene="Ampicillin" CDS 4540..5400 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5555..6174 /label="pBR322_origin" misc_feature 6390..6416 /label="lacI.C" promoter 6483..6512 /label="lac_promoter" misc_feature 6547..6565 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGATC GCCATCTCGC GCCCGTGCCT 3361 CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC 3421 ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT 3481 TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA 3541 AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA 3601 CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT 3661 AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT 3721 TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT 3781 TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT 3841 TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC 3901 TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA 3961 GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT 4021 TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC 4081 GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 4141 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 4201 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 4261 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 4321 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 4381 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 4441 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 4501 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 4561 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 4621 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 4681 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 4741 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 4801 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 4861 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 4921 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 4981 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 5041 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 5101 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 5161 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 5221 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 5281 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 5341 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 5401 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 5461 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 5521 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 5581 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 5641 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 5701 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 5761 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 5821 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 5881 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 5941 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 6001 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 6061 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 6121 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 6181 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 6241 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 6301 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 6361 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 6421 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 6481 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 6541 CACACAGGAA ACAGCT // 1439 LOCUS ADDGENE_1439 6555 bp DNA circular SYN 01/03/2005 DEFINITION pPD22_04 complete plasmid sequence KEYWORDS . SOURCE pPD22_04 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1439 at www.addgene.org FEATURES Location/Qualifiers source 1..6555 /organism="Addgene plasmid pPD22_04" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..138 /label="rf22.04" misc_feature 153..179 /label="SV40.NLS" CDS 153..3317 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 270..3314 /label="lacZ" /gene="lacZ" promoter 4469..4497 /label="Amp_promoter" gene 4539..5399 /label="Ampicillin" /gene="Ampicillin" CDS 4539..5399 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5554..6173 /label="pBR322_origin" misc_feature 6389..6415 /label="lacI.C" promoter 6482..6511 /label="lac_promoter" misc_feature 6546..6564 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GAGGGTACCG AGCTCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG 181 TACCGGTGGG TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT 241 TCAACGCGCT GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 301 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 361 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC 421 GCTTTGCCTG GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC 481 CTGAGGCCGA TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA 541 TCTACACCAA CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC 601 CGACGGGTTG TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA 661 CGCGAATTAT TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG 721 TCGGTTACGG CCAGGACAGT CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG 781 CCGGAGAAAA CCGCCTCGCG GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG 841 ATCAGGATAT GTGGCGGATG AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA 901 CTACACAAAT CAGCGATTTC CATGTTGCCA CTCGCTTTAA TGATGATTTC AGCCGCGCTG 961 TACTGGAGGC TGAAGTTCAG ATGTGCGGCG AGTTGCGTGA CTACCTACGG GTAACAGTTT 1021 CTTTATGGCA GGGTGAAACG CAGGTCGCCA GCGGCACCGC GCCTTTCGGC GGTGAAATTA 1081 TCGATGAGCG TGGTGGTTAT GCCGATCGCG TCACACTACG TCTGAACGTC GAAAACCCGA 1141 AACTGTGGAG CGCCGAAATC CCGAATCTCT ATCGTGCGGT GGTTGAACTG CACACCGCCG 1201 ACGGCACGCT GATTGAAGCA GAAGCCTGCG ATGTCGGTTT CCGCGAGGTG CGGATTGAAA 1261 ATGGTCTGCT GCTGCTGAAC GGCAAGCCGT TGCTGATTCG AGGCGTTAAC CGTCACGAGC 1321 ATCATCCTCT GCATGGTCAG GTCATGGATG AGCAGACGAT GGTGCAGGAT ATCCTGCTGA 1381 TGAAGCAGAA CAACTTTAAC GCCGTGCGCT GTTCGCATTA TCCGAACCAT CCGCTGTGGT 1441 ACACGCTGTG CGACCGCTAC GGCCTGTATG TGGTGGATGA AGCCAATATT GAAACCCACG 1501 GCATGGTGCC AATGAATCGT CTGACCGATG ATCCGCGCTG GCTACCGGCG ATGAGCGAAC 1561 GCGTAACGCG AATGGTGCAG CGCGATCGTA ATCACCCGAG TGTGATCATC TGGTCGCTGG 1621 GGAATGAATC AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG 1681 ATCCTTCCCG CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA 1741 TTTGCCCGAT GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT 1801 CCATCAAAAA ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATCCTT TGCGAATACG 1861 CCCACGCGAT GGGTAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG TTTCGTCAGT 1921 ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG ATTAAATATG 1981 ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG CCGAACGATC 2041 GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA GCGCTGACGG 2101 AAGCAAAACA CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA 2161 CCAGCGAATA CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG 2221 ATGGTAAGCC GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT 2281 TGATTGAACT GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC 2341 GCGTAGTGCA ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCCTGGCAGC 2401 AGTGGCGTCT GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC CGCGTCCCAC GCCATCCCGC 2461 ATCTGACCAC CAGCGAAATG GATTTTTGCA TCGAGCTGGG TAATAAGCGT TGGCAATTTA 2521 ACCGCCAGTC AGGCTTTCTT TCACAGATGT GGATTGGCGA TAAAAAACAA CTGCTGACGC 2581 CGCTGCGCGA TCAGTTCACC CGTGCACCGC TGGATAACGA CATTGGCGTA AGTGAAGCGA 2641 CCCGCATTGA CCCTAACGCC TGGGTCGAAC GCTGGAAGGC GGCGGGCCAT TACCAGGCCG 2701 AAGCAGCGTT GTTGCAGTGC ACGGCAGATA CACTTGCTGA TGCGGTGCTG ATTACGACCG 2761 CTCACGCGTG GCAGCATCAG GGGAAAACCT TATTTATCAG CCGGAAAACC TACCGGATTG 2821 ATGGTAGTGG TCAAATGGCG ATTACCGTTG ATGTTGAAGT GGCGAGCGAT ACACCGCATC 2881 CGGCGCGGAT TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC AGAGCGGGTA AACTGGCTCG 2941 GATTAGGGCC GCAAGAAAAC TATCCCGACC GCCTTACTGC CGCCTGTTTT GACCGCTGGG 3001 ATCTGCCATT GTCAGACATG TATACCCCGT ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT 3061 GCGGGACGCG CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC CAGTTCAACA 3121 TCAGCCGCTA CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG CTGCACGCGG 3181 AAGAAGGCAC ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC GACGACTCCT 3241 GGAGCCCGTC AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT TACCAACTTG 3301 TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGATCG CCATCTCGCG CCCGTGCCTC 3361 TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA 3421 CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT 3481 TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA 3541 AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC 3601 AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA 3661 GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT 3721 TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT 3781 TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT 3841 TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT 3901 AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG 3961 GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT 4021 TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG 4081 GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 4141 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 4201 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 4261 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 4321 AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 4381 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 4441 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 4501 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 4561 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 4621 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 4681 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 4741 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 4801 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 4861 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 4921 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 4981 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 5041 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 5101 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 5161 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 5221 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 5281 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 5341 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 5401 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 5461 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 5521 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 5581 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 5641 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 5701 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 5761 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 5821 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 5881 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 5941 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6001 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6061 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6121 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6181 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 6241 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 6301 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 6361 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 6421 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 6481 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 6541 ACACAGGAAA CAGCT // 1440 LOCUS ADDGENE_1440 6560 bp DNA circular SYN 01/03/2005 DEFINITION pPD22_11 complete plasmid sequence KEYWORDS . SOURCE pPD22_11 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1440 at www.addgene.org FEATURES Location/Qualifiers source 1..6560 /organism="Addgene plasmid pPD22_11" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..139 /label="rf22.11" misc_feature 158..184 /label="SV40.NLS" CDS 158..3322 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 275..3319 /label="lacZ" /gene="lacZ" promoter 4474..4502 /label="Amp_promoter" CDS 4544..5404 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4544..5404 /label="Ampicillin" /gene="Ampicillin" rep_origin 5559..6178 /label="pBR322_origin" misc_feature 6394..6420 /label="lacI.C" promoter 6487..6516 /label="lac_promoter" misc_feature 6551..6569 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 181 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 241 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 301 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 361 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 421 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 481 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 541 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 601 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 661 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 721 CTGGGTCGGT TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT 781 ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT 841 GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA 901 ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG 961 CGCTGTACTG GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 1021 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 1081 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 1141 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 1201 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 1261 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 1321 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATATCCT 1381 GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT 1441 GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC 1501 CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG 1561 CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC 1621 GCTGGGGAAT GAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT GGATCAAATC 1681 TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA CGGCCACCGA 1741 TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG CTGTGCCGAA 1801 ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA TCCTTTGCGA 1861 ATACGCCCAC GCGATGGGTA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG 1921 TCAGTATCCC CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA 1981 ATATGATGAA AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA 2041 CGATCGCCAG TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCGCT 2101 GACGGAAGCA AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA 2161 AGTGACCAGC GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC 2221 GCTGGATGGT AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA 2281 ACAGTTGATT GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC 2341 AGTACGCGTA GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCCTG 2401 GCAGCAGTGG CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT CCCACGCCAT 2461 CCCGCATCTG ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA AGCGTTGGCA 2521 ATTTAACCGC CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA AACAACTGCT 2581 GACGCCGCTG CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG GCGTAAGTGA 2641 AGCGACCCGC ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG GCCATTACCA 2701 GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC 2761 GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG 2821 GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC 2881 GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG 2941 GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG 3001 CTGGGATCTG CCATTGTCAG ACATGTATAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT 3061 GCGCTGCGGG ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT 3121 CAACATCAGC CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA 3181 CGCGGAAGAA GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA 3241 CTCCTGGAGC CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 3301 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GATCGCCATC TCGCGCCCGT 3361 GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC 3421 TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT 3481 TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA 3541 TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC 3601 TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA 3661 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC 3721 AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA 3781 AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC 3841 TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC 3901 TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT 3961 TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG 4021 GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT 4081 AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA 4141 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 4201 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA 4261 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA 4321 AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT 4381 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG 4441 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA 4501 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT 4561 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 4621 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA 4681 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT 4741 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA 4801 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT 4861 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 4921 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT 4981 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA 5041 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC 5101 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT 5161 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 5221 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC 5281 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC 5341 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG 5401 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 5461 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 5521 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 5581 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 5641 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 5701 AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 5761 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 5821 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 5881 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 5941 CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 6001 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 6061 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 6121 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 6181 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 6241 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 6301 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 6361 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA 6421 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC 6481 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA 6541 ATTTCACACA GGAAACAGCT // 1441 LOCUS ADDGENE_1441 7242 bp DNA circular SYN 01/03/2005 DEFINITION pPD26_77 complete plasmid sequence KEYWORDS . SOURCE pPD26_77 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1441 at www.addgene.org FEATURES Location/Qualifiers source 1..7242 /organism="Addgene plasmid pPD26_77" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" misc_feature 3921..3944 /label="glp1.3'" promoter complement(4528..4547) /label="T3_promoter" misc_feature complement(4564..4582) /label="M13r_primer" misc_feature complement(4713..4739) /label="lacI.C" promoter 5156..5184 /label="Amp_promoter" CDS 5226..6086 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5226..6086 /label="Ampicillin" /gene="Ampicillin" rep_origin 6241..6860 /label="pBR322_origin" misc_feature 7076..7102 /label="lacI.C" promoter 7169..7198 /label="lac_promoter" misc_feature 7233..7251 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGCGAAAC AAATCATCTG ACACCACCAC CGTCTGATGG ATCGTTCTCA TCTCCGTCTC 3301 CACATTATTA TCCGACGACT ACATCGACAC CGAATCGAAT GGAAACAAGT CCGGAGTACA 3361 TGTTTAACCA TGAAATGGTG GGTAGATGAT TATTAAAATG TTTAAGAAAA TTAAATAATT 3421 TGTTTTAGGC ACCACCGGTC AATGCGATGT GGTATACTAC ACCACCTCCT TATCAAGATC 3481 CAAACTATCG TCATGTGCCT CCAAATACTG CATTTCAAAA TGCAGAGCAA ATGAATGGCT 3541 CCTTCTACTG TTAATCTATT TAATTCATTA ATTTTTCATT TATTGACTGT ATCCCGGATG 3601 TTTCTTGTCC TCCCAACATA TCTCCTAACT GCTCGGTTCA TTTTAAATAT GCTCATCTCA 3661 CTACATCACC CAGACACTGG TCCCCACAGA GTTTTTTGTA TACTATTTCG GGTCATTTTT 3721 CTTATTCTAG ACTAATATTG TAAGCTATAA GTTGTAGAAT AATTATTGAT CCAAATCAGA 3781 TTAAGAGTAT AAGCTTTGTT TTTTCTCCTT TTCTTTATAA CTTGTTACAA TTTTTGAAAT 3841 TCCCTTTTTT GACAGGCTTT TATTACACTG TAACTGTGTT TCTTATCTTG CAAACATTTA 3901 ATGAATTGTA ATTCTTTAGT ATCTTGAGGG CTTTTTGTTT TTCGAATTAT TGAAGCTCAA 3961 AGTTCCAGTT TTACTACGAT CCAGCGAATT CTCCTCATTT CGATCCGATG CAATTGACTT 4021 CAGATCAATA TTGGTTGCCT GAAAGAAATA ATTGTGAGCA TTTTTGTCAA AAAACAGAGA 4081 ACTCACCATT CTCGAGGCTC CCGTTCCAGG AGCAGTACTT GGTGATGGAC ACGTAGATTG 4141 ATTAAACCAA ACCAAAGGTT CTTTCAGAGT CAACTTACAG CCTCGAGCGT AGTCCGTGAT 4201 AGCTTCTCGC AGAACACTGA AAATTGGAAA TTTATTGGAA TAAAAACTTT TTCTGCACTT 4261 TATAGAATAA AAAAATCATG AATTTACCCG AATTTAACCT CCGAATCGTA GACCAAATTG 4321 TCCAAGTAGA TGGAAATCAC CTTGAACATC GGATGTTTTT CATATGCTGA AAATAAATTA 4381 ATGAATTTAT GTAATTTTTT AAATAATTAC TTTTCAATTT GGTGAACAAT TCCTGCTTCT 4441 TTGCATAGGC ATCTGGACGA GTGAGTCCTT TCCAATCAAT CAATGTGGTG TCGACCTCGA 4501 GGGGGGGCCC GGTACCCAGC TTTTGTTCCC TTTAGTGAGG GTTAATTCCG AGCTTGGCGT 4561 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 4621 TAGGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGG TAACTCACAT 4681 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 4741 AATGAATCGG CCAACGCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG 4801 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA 4861 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG 4921 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG 4981 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG 5041 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG 5101 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA 5161 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 5221 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 5281 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG 5341 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 5401 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT 5461 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 5521 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG 5581 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA 5641 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC 5701 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA 5761 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 5821 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC 5881 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG 5941 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA 6001 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG 6061 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA 6121 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC 6181 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 6241 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 6301 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 6361 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT 6421 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 6481 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 6541 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 6601 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG 6661 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 6721 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 6781 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 6841 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 6901 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT 6961 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG 7021 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA 7081 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA 7141 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT 7201 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1442 LOCUS ADDGENE_1442 4427 bp DNA circular SYN 01/03/2005 DEFINITION pPD30_35 complete plasmid sequence KEYWORDS . SOURCE pPD30_35 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1442 at www.addgene.org FEATURES Location/Qualifiers source 1..4427 /organism="Addgene plasmid pPD30_35" /mol_type="other DNA" misc_feature 583..606 /label="unc54.5'" misc_feature 624..665 /label="SynIVS.A" misc_feature 672..689 /label="rf21.28" promoter 2341..2369 /label="Amp_promoter" gene 2411..3271 /label="Ampicillin" /gene="Ampicillin" CDS 2411..3271 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3426..4045 /label="pBR322_origin" misc_feature 4261..4287 /label="lacI.C" promoter 4354..4383 /label="lac_promoter" misc_feature 4418..4436 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGTGAGT ATTTTCGGTT TTTCACTGGT 61 TCTCTGTAAA AAGAGTAATG CAAAGGGCAA GTTAACTTAG GTCGTAAATG TATTGAATTT 121 GCTTAAAATC TGAAGATCTA GTGGTGAACC GTGGAAGATT ATCAAGAGGA GGCTGAAGAT 181 CTGTTTAAGA ACCATTAATC AAACTGGTAT TCTATTTTCA CTGGTTGTAT GTAAACATTC 241 TATCTTATTC CTTTTATCAC TGTTCTGCAC TTTCCTATAA AAAAAGTTGA CCGACCGTAC 301 TCTCTGAATT CATTTTTCCC GATCTTACCA ACTCCCGATC TATCTCTATC CCTGGTTTTT 361 TCTTCGTGCT CCAATGGAAT TCTTGAGACT TCCACTATCT TCTCTGGCAC CCTCCACTAC 421 GCGTAGGCGT CTCTCGCTTC GTGTATTCCC GGGAAGCCGG GTTCCCGTCT CTCCCGCCGC 481 TGCCGCTGCC GCACACAGCT TTACACCTCG TAGAATCCCC AAAGAGGGGC GTGGCTTGCG 541 GGTGCCAACA TCCTCCTGCC GAGGAAGAAG CAGGCACTCA TCACTCGCAT CATCAACCTC 601 GGGATTGGCC AAAGGACCCA AAGGTATGTT TCGAATGATA CTAACATAAC ATAGAACATT 661 TTCAGGAGGA CCCTTGGCTA GCGTCGACGG TACCATGGTA TTGATATCTG AGCTCCGCAT 721 CGGCCGCTGT CATCAGATCG CCATCTCGCG CCCGTGCCTC TGACTTCTAA GTCCAATTAC 781 TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT TTGTTATTAT 841 CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT CTAACAATGA 901 AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA AATTGTGCTC 961 CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT GTACACTTCT 1021 TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG CACACAAAAT 1081 ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC GCACGTCTGG 1141 GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT TTTGGAATTT 1201 TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC 1261 TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA GTATTGATGA 1321 GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG 1381 AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA TGACAGAATA 1441 CATTCCCAAT ATACCAAACA TAACTGTTTA AAATTAAACA TTTTTCTAAA TTTTATATGA 1501 TTTCTTTTAA ATTTGCAAAA ATTACTTAAA TTTGAATTCC CGCGCAAATG AGTGACTTCA 1561 TTTTCTGCAT TATTGTGTTT TCCGGCTATA TTAATAGGTA TTTGTTTGTG TTTTTCTTTA 1621 TTTTATGATT CGAACTCCAA TTTGTAAATT TTCGAACATA TTTCCCTAAA GAAAAAATAT 1681 GATTAATCTG GAAAAATTGG AAAATTATTT TTCAAATAAA AAACAAAGAA AAAAATGAAG 1741 AAAAACCTAT TAGTTTGGCC ATAAAACGCA AAAATGTCGA AAATGACGTC ACTCATCTGC 1801 GCGGGAAATC AAGAATAATT CGGCCTTTTT TATTTTTTTG GAAAATCGTA AAACATTTAG 1861 AAAAATTTTT TAATAGTTAT AGTGGGACTG TATTCTGTCA TTTAGGGCAA AAGCCAGAGA 1921 CGCTACTCCA CCGTTGGGGG ATCCACTAGT CGGCCGTACG GGCCCTTTCG TCTCGCGCGT 1981 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT 2041 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG 2101 TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG 2161 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG GCCTTAAGGG 2221 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC 2281 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 2341 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA 2401 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT 2461 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA 2521 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG 2581 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC 2641 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA 2701 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT 2761 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT 2821 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT 2881 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA 2941 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT 3001 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC 3061 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA 3121 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT 3181 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA 3241 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT 3301 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA 3361 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT 3421 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 3481 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 3541 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA 3601 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3661 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3721 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 3781 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA 3841 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 3901 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 3961 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 4021 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4081 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT 4141 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA 4201 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA 4261 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA 4321 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT 4381 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1443 LOCUS ADDGENE_1443 4812 bp DNA circular SYN 01/03/2005 DEFINITION pPD30_38 complete plasmid sequence KEYWORDS . SOURCE pPD30_38 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1443 at www.addgene.org FEATURES Location/Qualifiers source 1..4812 /organism="Addgene plasmid pPD30_38" /mol_type="other DNA" misc_feature 120..150 /label="unc54.enh" misc_feature 968..991 /label="unc54.5'" misc_feature 1009..1050 /label="SynIVS.A" misc_feature 1057..1074 /label="rf21.28" promoter 2726..2754 /label="Amp_promoter" CDS 2796..3656 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2796..3656 /label="Ampicillin" /gene="Ampicillin" rep_origin 3811..4430 /label="pBR322_origin" misc_feature 4646..4672 /label="lacI.C" promoter 4739..4768 /label="lac_promoter" misc_feature 4803..4821 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGTCTTCT TCTAAATTCC CATAAAATCC CGAAACTCCT 61 TCCCTCTATC TTCTTTTTCT TCTCGTTTTC AAATGTTTCT CTCTATCCCA TTCTCTCATC 121 AATTGAGTGG GATGAGGCTA TCTCTGCCTC TCTTCTGAAT CTCTGAACCA TCTTACATTA 181 CACTGTGGAT GACGAGCCCC ACAGGCTCCC TTGCATCAGA TACTGCCATT GGGGATGGCA 241 AAGAAGAGAG AAGGTATTGT GAGGATATAT TTTTCTAAGA AAAAACGTTT GAAGAAAAGA 301 AGATGAAGAA GATCTGCTTG ATTCATTGCA CAAGTTAGAA GTAACAGGGG TCTATATTTC 361 GAAGAACTTA AAGGGAATGC AACTGAACAT AAAATTAAAC AAAGGGATTG AATCCTGCAG 421 TGAGTATTTT CGGTTTTTCA CTGGTTCTCT GTAAAAAGAG TAATGCAAAG GGCAAGTTAA 481 CTTAGGTCGT AAATGTATTG AATTTGCTTA AAATCTGAAG ATCTAGTGGT GAACCGTGGA 541 AGATTATCAA GAGGAGGCTG AAGATCTGTT TAAGAACCAT TAATCAAACT GGTATTCTAT 601 TTTCACTGGT TGTATGTAAA CATTCTATCT TATTCCTTTT ATCACTGTTC TGCACTTTCC 661 TATAAAAAAA GTTGACCGAC CGTACTCTCT GAATTCATTT TTCCCGATCT TACCAACTCC 721 CGATCTATCT CTATCCCTGG TTTTTTCTTC GTGCTCCAAT GGAATTCTTG AGACTTCCAC 781 TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA TTCCCGGGAA 841 GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA CCTCGTAGAA 901 TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA AGAAGCAGGC 961 ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA 1021 TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGCTAGCGTC GACGGTACCA 1081 TGGTATTGAT ATCTGAGCTC CGCATCGGCC GCTGTCATCA GATCGCCATC TCGCGCCCGT 1141 GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC 1201 TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT 1261 TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA 1321 TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC 1381 TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA 1441 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC 1501 AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA 1561 AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC 1621 TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC 1681 TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT 1741 TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG 1801 GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT GTTTAAAATT 1861 AAACATTTTT CTAAATTTTA TATGATTTCT TTTAAATTTG CAAAAATTAC TTAAATTTGA 1921 ATTCCCGCGC AAATGAGTGA CTTCATTTTC TGCATTATTG TGTTTTCCGG CTATATTAAT 1981 AGGTATTTGT TTGTGTTTTT CTTTATTTTA TGATTCGAAC TCCAATTTGT AAATTTTCGA 2041 ACATATTTCC CTAAAGAAAA AATATGATTA ATCTGGAAAA ATTGGAAAAT TATTTTTCAA 2101 ATAAAAAACA AAGAAAAAAA TGAAGAAAAA CCTATTAGTT TGGCCATAAA ACGCAAAAAT 2161 GTCGAAAATG ACGTCACTCA TCTGCGCGGG AAATCAAGAA TAATTCGGCC TTTTTTATTT 2221 TTTTGGAAAA TCGTAAAACA TTTAGAAAAA TTTTTTAATA GTTATAGTGG GACTGTATTC 2281 TGTCATTTAG GGCAAAAGCC AGAGACGCTA CTCCACCGTT GGGGGATCCA CTAGTCGGCC 2341 GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA 2401 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA 2461 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA 2521 GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA 2581 ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA 2641 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 2701 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 2761 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 2821 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 2881 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 2941 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 3001 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 3061 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 3121 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 3181 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 3241 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 3301 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 3361 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 3421 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 3481 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 3541 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 3601 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT 3661 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA 3721 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT 3781 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 3841 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 3901 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 3961 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 4021 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 4081 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 4141 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 4201 GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 4261 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 4321 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 4381 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 4441 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT 4501 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA 4561 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC 4621 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG 4681 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT 4741 TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA 4801 CAGGAAACAG CT // 1444 LOCUS ADDGENE_1444 5087 bp DNA circular SYN 01/03/2005 DEFINITION pPD30_69 complete plasmid sequence KEYWORDS . SOURCE pPD30_69 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1444 at www.addgene.org FEATURES Location/Qualifiers source 1..5087 /organism="Addgene plasmid pPD30_69" /mol_type="other DNA" misc_feature 74..98 /label="myo2.-1090" misc_feature 1162..1185 /label="myo2.5'" misc_feature 1259..1300 /label="SynIVS.A" misc_feature 1307..1324 /label="rf21.28" promoter 3001..3029 /label="Amp_promoter" CDS 3071..3931 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3071..3931 /label="Ampicillin" /gene="Ampicillin" rep_origin 4086..4705 /label="pBR322_origin" misc_feature 4921..4947 /label="lacI.C" promoter 5014..5043 /label="lac_promoter" misc_feature 5078..5096 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAGCTT 61 GCATGCCTGC AGGTCGAGGC ATTTGAATTG GGGGTGGTGG ACAGTAACTG TCTGTAATAA 121 TAATTACTCC TGACCAGGTT GCAATTCGAG TTTTGATAAG CATAATTATA CCTTGTACAT 181 TGTGGGTTTT GTGCTGTGGA CGTTTTATTG TGACATCCCC ATAAGCTACA AGAACAAAAA 241 TGAAATgTAA AAGTATTGAA AACGTCGTAC ATTTTATATC TGAGTAGTAT CCTTTGCTTT 301 AAATGTCCAT AAAAcTAATT TTATAATCAA TAAAACAACG TTTGTAAATC AACTGAGTTT 361 ACAAGTAGAG ACATTGAGGG ATACTTTCAC TATGCTAAAG TGATAATCGA CCAAATAATA 421 ACTtCACTTT GGTATTTATT CCTGTCTTAT AAaTGTTATG TATGAATTAA ATTCATATGC 481 ATATGGCTCA CTCTGACAAt ttttAATAAT CTTCCAGATC AATATTGACT ACCGATGCGG 541 GTGGTCTTTT GCTTTGAATT CTGCTGAACT TTACACCCCG AACAGCAATG TGTGCTTCAG 601 CcTAAAAAAA AGTAAGTGTG TTAATCAGTg CCCCCGATTC TTCATTTTTT GCCCCTCTCT 661 CCCGTTTCGT CGGCAAAAGA AGAGAAAATA AAGATAAGTC TCAAGATAGG TTGGTAATCG 721 CTAAAGTGGT TGTGTGGATA AGAGTAGCAA AATGGCAGGA AGAGCACTTT GCGCGCACAC 781 ACTGTACTCA TTGTTCTGGA TAAAATTCTC TCGTTGTTTG CCGTCGGATG TCTGCCTCTC 841 TGCATTGAGC CGGCTTCTTC ACTATCTTTA GTTAACCTAA AATGCCGTTT CTTTTCTCGT 901 ATCCCcACTA TcCCGTTGAG GTTCTCTGCT CTCTTCGCTC CCTACCGCCA GCGAGCAACT 961 ATCCGTGGGG GCGCCTTGCT CGGAAGATGG GGGGGAAGAA AGAAGATTTT TGCTATTTGC 1021 ACTTGAGAAA GAGACTTTTC CTGCGTCGAT GGTTAGAGAA CAGTGTGCAG ACACTTTTCA 1081 GCTACCTAGA atTACAatTG GATATCCCCG CCTCCCAATC CACCCACCCA GGGAAAAAGA 1141 AGGGCTCGCC GAAAATCAAA GTTATCTCCA GGCTCGCGCA TCCCACCGAG CGGTTGACTT 1201 CTCTCCACCA CTTTTCATTT TAACCCTCGG GGTACGGGAT TGGCCAAAGG ACCCAAAGGT 1261 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGCTAGCGTC 1321 GACGGTACCA TGGTATTGAT ATCTGAGCTC CGCATCGGCC GCTGTCATCA GATCGCCATC 1381 TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT 1441 CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG 1501 TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT 1561 TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA 1621 TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT 1681 TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT 1741 TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT 1801 CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA 1861 TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC 1921 GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA 1981 AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA 2041 GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT 2101 GTTTAAAATT AAACATTTTT CTAAATTTTA TATGATTTCT TTTAAATTTG CAAAAATTAC 2161 TTAAATTTGA ATTCCCGCGC AAATGAGTGA CTTCATTTTC TGCATTATTG TGTTTTCCGG 2221 CTATATTAAT AGGTATTTGT TTGTGTTTTT CTTTATTTTA TGATTCGAAC ATATTTCCCT 2281 AAAGAAAAAA TATGATTAAT CTGGAAAAAT TGGAAAATTA TTTTTCAAAT AAAAAACAAA 2341 GAAAAAAATG AAGAAAAACC TATTAGTTTG GCCATAAAAC GCAAAAATGT CGAAAATGAC 2401 GTCACTCATC TGCGCGGGAA ATCAAGAATA ATTCGGCCTT TTTTATTTTT TTGGAAAATC 2461 GTAAAACATT TAGAAAAATT TTTTAATAGT TATATGGGAC TTATTCTGCA TTTAGGGCAA 2521 AAGCCAGAGA CTACTCCACC GTTAACATGA ATTATGAATA TTATTGCGAC AAGACCCAAA 2581 CATTGATAAA CCCAAATCTA GCCTACTAGT CGGCCGTACG GGCCCTTTCG TCTCGCGCGT 2641 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT 2701 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG 2761 TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG 2821 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG GCCTTAAGGG 2881 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC 2941 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 3001 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA 3061 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT 3121 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA 3181 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG 3241 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC 3301 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA 3361 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT 3421 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT 3481 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT 3541 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA 3601 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT 3661 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC 3721 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA 3781 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT 3841 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA 3901 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT 3961 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA 4021 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT 4081 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 4141 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 4201 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA 4261 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 4321 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 4381 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 4441 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA 4501 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 4561 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 4621 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 4681 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4741 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT 4801 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA 4861 GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA 4921 ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA 4981 TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT 5041 GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1445 LOCUS ADDGENE_1445 6613 bp DNA circular SYN 01/03/2005 DEFINITION pPD34_110 complete plasmid sequence KEYWORDS . SOURCE pPD34_110 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1445 at www.addgene.org FEATURES Location/Qualifiers source 1..6613 /organism="Addgene plasmid pPD34_110" /mol_type="other DNA" CDS 1..3375 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPRESWQRALVLLIVLLFIVIFVITVLFVIRSNKVPVGEDQKQHLELSRDIAQRFNA LYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR FAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTE NPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSA FLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFND DFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTL RLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPL LIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKW LSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDEN GNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVT SEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLT VRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKR WQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAA GHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNID GFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 160..225 /label="SynTmD" gene 328..3372 /label="lacZ" /gene="lacZ" promoter 4527..4555 /label="Amp_promoter" gene 4597..5457 /label="Ampicillin" /gene="Ampicillin" CDS 4597..5457 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5612..6231 /label="pBR322_origin" misc_feature 6447..6473 /label="lacI.C" promoter 6540..6569 /label="lac_promoter" misc_feature 6604..6622 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 121 GAGGACCCTT GGAGGGTACC TCGAGAAAGC TGGCAAAGGG CTCTTGTCCT GCTAATCGTA 181 CTACTCTTCA TCGTCATCTT CGTTATTACT GTTTTGTTCG TCATAAGATC TAACAAGGTA 241 CCGGTGGGTG AAGACCAGAA ACAGCACCTC GAACTGAGCC GCGATATTGC CCAGCGTTTC 301 AACGCGCTGT ATGGCGAGAT CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT 361 GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC 421 GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC 481 TTTGCCTGGT TTCCGGCACC AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT 541 GAGGCCGATA CTGTCGTCGT CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC 601 TACACCAACG TGACCTATCC CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG 661 ACGGGTTGTT ACTCGCTCAC ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG 721 CGAATTATTT TTGATGGCGT TAACTCGGCG TTTCATCTGT GGTGCAACGG GCGCTGGGTC 781 GGTTACGGCC AGGACAGTCG TTTGCCGTCT GAATTTGACC TGAGCGCATT TTTACGCGCC 841 GGAGAAAACC GCCTCGCGGT GATGGTGCTG CGCTGGAGTG ACGGCAGTTA TCTGGAAGAT 901 CAGGATATGT GGCGGATGAG CGGCATTTTC CGTGACGTCT CGTTGCTGCA TAAACCGACT 961 ACACAAATCA GCGATTTCCA TGTTGCCACT CGCTTTAATG ATGATTTCAG CCGCGCTGTA 1021 CTGGAGGCTG AAGTTCAGAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 1081 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 1141 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 1201 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 1261 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 1321 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 1381 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATAT CCTGCTGATG 1441 AAGCAGAACA ACTTTAACGC CGTGCGCTGT TCGCATTATC CGAACCATCC GCTGTGGTAC 1501 ACGCTGTGCG ACCGCTACGG CCTGTATGTG GTGGATGAAG CCAATATTGA AACCCACGGC 1561 ATGGTGCCAA TGAATCGTCT GACCGATGAT CCGCGCTGGC TACCGGCGAT GAGCGAACGC 1621 GTAACGCGAA TGGTGCAGCG CGATCGTAAT CACCCGAGTG TGATCATCTG GTCGCTGGGG 1681 AATGAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 1741 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 1801 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 1861 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATCCTTTG CGAATACGCC 1921 CACGCGATGG GTAACAGTCT TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT 1981 CCCCGTTTAC AGGGCGGCTT CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT 2041 GAAAACGGCA ACCCGTGGTC GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC 2101 CAGTTCTGTA TGAACGGTCT GGTCTTTGCC GACCGCACGC CGCATCCAGC GCTGACGGAA 2161 GCAAAACACC AGCAGCAGTT TTTCCAGTTC CGTTTATCCG GGCAAACCAT CGAAGTGACC 2221 AGCGAATACC TGTTCCGTCA TAGCGATAAC GAGCTCCTGC ACTGGATGGT GGCGCTGGAT 2281 GGTAAGCCGC TGGCAAGCGG TGAAGTGCCT CTGGATGTCG CTCCACAAGG TAAACAGTTG 2341 ATTGAACTGC CTGAACTACC GCAGCCGGAG AGCGCCGGGC AACTCTGGCT CACAGTACGC 2401 GTAGTGCAAC CGAACGCGAC CGCATGGTCA GAAGCCGGGC ACATCAGCGC CTGGCAGCAG 2461 TGGCGTCTGG CGGAAAACCT CAGTGTGACG CTCCCCGCCG CGTCCCACGC CATCCCGCAT 2521 CTGACCACCA GCGAAATGGA TTTTTGCATC GAGCTGGGTA ATAAGCGTTG GCAATTTAAC 2581 CGCCAGTCAG GCTTTCTTTC ACAGATGTGG ATTGGCGATA AAAAACAACT GCTGACGCCG 2641 CTGCGCGATC AGTTCACCCG TGCACCGCTG GATAACGACA TTGGCGTAAG TGAAGCGACC 2701 CGCATTGACC CTAACGCCTG GGTCGAACGC TGGAAGGCGG CGGGCCATTA CCAGGCCGAA 2761 GCAGCGTTGT TGCAGTGCAC GGCAGATACA CTTGCTGATG CGGTGCTGAT TACGACCGCT 2821 CACGCGTGGC AGCATCAGGG GAAAACCTTA TTTATCAGCC GGAAAACCTA CCGGATTGAT 2881 GGTAGTGGTC AAATGGCGAT TACCGTTGAT GTTGAAGTGG CGAGCGATAC ACCGCATCCG 2941 GCGCGGATTG GCCTGAACTG CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA CTGGCTCGGA 3001 TTAGGGCCGC AAGAAAACTA TCCCGACCGC CTTACTGCCG CCTGTTTTGA CCGCTGGGAT 3061 CTGCCATTGT CAGACATGTA TACCCCGTAC GTCTTCCCGA GCGAAAACGG TCTGCGCTGC 3121 GGGACGCGCG AATTGAATTA TGGCCCACAC CAGTGGCGCG GCGACTTCCA GTTCAACATC 3181 AGCCGCTACA GTCAACAGCA ACTGATGGAA ACCAGCCATC GCCATCTGCT GCACGCGGAA 3241 GAAGGCACAT GGCTGAATAT CGACGGTTTC CATATGGGGA TTGGTGGCGA CGACTCCTGG 3301 AGCCCGTCAG TATCGGCGGA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 3361 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGATCGCC ATCTCGCGCC CGTGCCTCTG 3421 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 3481 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 3541 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 3601 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 3661 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 3721 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 3781 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 3841 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 3901 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 3961 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 4021 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 4081 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 4141 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 4201 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 4261 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 4321 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 4381 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 4441 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 4501 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 4561 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 4621 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 4681 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 4741 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 4801 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 4861 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 4921 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 4981 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 5041 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 5101 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 5161 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 5221 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 5281 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 5341 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 5401 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 5461 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 5521 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 5581 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 5641 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 5701 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 5761 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 5821 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 5881 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 5941 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 6001 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 6061 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 6121 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 6181 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 6241 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 6301 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 6361 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 6421 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 6481 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 6541 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 6601 ACAGGAAACA GCT // 1446 LOCUS ADDGENE_1446 5156 bp DNA circular SYN 01/03/2005 DEFINITION pPD47_52 complete plasmid sequence KEYWORDS . SOURCE pPD47_52 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1446 at www.addgene.org FEATURES Location/Qualifiers source 1..5156 /organism="Addgene plasmid pPD47_52" /mol_type="other DNA" promoter 6..25 /label="Sp6_promoter" promoter complement(75..93) /label="T7_promoter" misc_feature 345..374 /label="Ori.Lam>>" CDS complement(446..1147) /label="Orf1" /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF SQLLATFPASLANRDQNEVNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK IAEIKAKFGLKGASV" CDS complement(1144..2043) /label="Orf0" /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ GGMFGPNKNISEWCIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ AGVTASKPKLDLTNTDWIYGVDL" misc_feature 3238..3267 /label="Ori.Lam<<" promoter 3487..3536 /label="NeoKan_promoter" CDS 3624..4286 /label="Orf2" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVATAI RT" gene 3627..4581 /label="Neomycin(w/ gaps)" /gene="Neomycin(w/ gaps)" ORIGIN 1 ATTAgattta ggtgacacta tagaagcttg gatccacgtg tcgactctag agcggccgcg 61 tatacgggcc ctctccctat agtgagtcgt attaATTGTA GCCGCGTTCT AACGACAATA 121 TGTACAAGCC TAATTGTGTA GCATCTGGCT TACTGAAGCA GACCCTATCA TCTCTCTCGT 181 AAACTGCCGT CAGAGTCGGT TTGGTTGGAC GAACCTTCTG AGTTTCTGGT AACGCCGTCC 241 CGCACCCGGA AATGGTCAGC GAACCAATCA GCAGGGTCAT CGCTAGAAAT CATCCTTAGC 301 GAAAGCTAAG GATTTTTTTT ATCTGAATTC TAGCCAGATC CCCGCTGATT TATGCTGGTT 361 ACTGTTGCGC CTGTTAGCGC GGCAACGTCC GGCGCACAGA AGCTATTATG CGTCCCCAGG 421 TAATGAATAA TTGCCTCTTT GCCCGTCATA CACTTGCTCC TTTCAGTCCG AACTTAGCTT 481 TGATTTCTGC GATCTTCGCC AGAGCCTGTG CACGATTTAG AGGTCTACCG CCCATGACAG 541 GAAGTTGTTT TACTGGTTCA GGGATCGCCT CACCACGGTT AATTCTCGCA GTCATATGGA 601 CAAGCTCATC TGCGGCCTTA CGGCGTAATT CCGCATCAGT AAGCGCATTG GCCCGCATGT 661 TCTGATACAG GTTGGTAACC AGCCAGTAGT GCGCGTTTGA TTTCCACGGA TAAGACTCCG 721 CATCCGGATA CAGGCCTCGC TTCCGGCAAT ACTCGTAAAC CATATCAACC AGCTCGCTGA 781 CGTTTGGCAG TCCGGCGGTA ACGGATGCTT CTTCCCGGCA CCATGCAACA AACTGCCCGG 841 GTGATGGCAG AAATGGTCGA TTCTGCCGAC GGGCTACGCG CATTCCTGCG TTAACCTGTT 901 CCATCGTGGT GATCCCGTTT TCCCGAAAAG CCAGAACCCA CTGGCGACGG ATTTCGTTCA 961 CTTCGTTCTG GTCACGGTTA GCCAGGCTCG CCGGGAAAGT TGCCAGTAAC TGGCTGAACA 1021 CACCGTTGAT GATCTGCGCT ACCTGCTGTA CCTGCGGCTT TTCGTCGTAC TGTTCCGGCA 1081 TGTTGTTGGC GATCCGACGC ATCTGCTCAC GGTCAAAGTT AACCATCTGT GCGGCGATGT 1141 TTTTCATAGA TCCACCCCGT AAATCCAGTC TGTGTTTGTC AGGTCGAGTT TTGGTTTGCT 1201 GGCTGTCACG CCTGCCTGTT GCTTGTTACG GTTGATTTCG AGTTGGGTCC ACTTATCGCG 1261 GAGTTTGGCC GGGCTCAGCA CGTTACCGGA CCAGAAGTTG TCCTGGCATG CCCAGCGGAA 1321 CAGCACACAC ATGTCGCGGT GGTTACGTCC GTCACGTTCA CGCATCAGGC GGATATCGTT 1381 AGCCCACCCA GCAAAATTCG GTTTTCTGGC TGATGGTGCG ATAGTCTTCA CCATGTCAAA 1441 CATCCACTCT GCGGCGGTCA GGTCTTCTGC TGTCCCCCAC TTGCTGCCGC TCTGAATTGC 1501 AGCATCCGGT TTCACCACAG AAAGGTCGTT TTCTGGCTGG TCAGAGGATT CGCCAGAATT 1561 CTCTGACGAA TAATCTTTTC TTTTTTCTTT TGTAATAGTG TCTTTTGTGT CCCCCTGTTT 1621 TGAGGGATAG CAATCCCCCA ATTTGAGGGA TGTTTTATCC CTCGTTTTAG GGGATTTTCC 1681 CTCGTTTTGA GGGATGCACC ATTCTGAGAT GTTTTTATTT GGTCCAAACA TGCCGCCTTG 1741 CTGCTTGATA ATATTCATTC TGACGAGTTC TAACTTGGCT TCATTGCACC GTTTGACAGG 1801 TAACTTTGTA ATCTCGCTAA GTTGAGAATC GGTGATTCTG TCCATTGGTT TATTCCACCC 1861 ATAGGTTTTA CGCAGAATGG CAAGCAGCAC TTTAAACTGT CGCTTGGTCA GATCTGCGCC 1921 CGAATAAGCC TCAAGCAGCA TATTTGATAG TCTGGCGTAA CCATCATCGA GATCTGCCAC 1981 ATTACGCTCC TGTCCGGCAA AGTTACCTCT GCCGAAGTTG AGTATTTTTG CTGTATTTGT 2041 CATAATGACT CCTGTTGATA GATCCAGTAA TGACCTCAGA ACTCCATCTG GATTTGTTCA 2101 GAACGCTCGG TTGCCGCCGG GCGTTTTTTA TTGGTGAGAA TCGCAGCAAC TTGTCGCGCC 2161 AATCGAGCCA TGTCGTCGTC AACGACCCCC CATTCAAGAA CAGCAAGCAG CATTGAGAAC 2221 TTTGGAATCC AGTCCCTCTT CCACCTGCTG ATCTGCGACT TATCAACGCC CACAGCTTCC 2281 GCTGTCTTCT CAGTTCCAAG CATTGCGATT TTGTTAAGCA ACGCACTCTC GATTCGTAGA 2341 GCCTCGTTGC GTTTGTTTGC ACGAACCATA TGTAAGTATT TCCTTAGATA ACAATTGATT 2401 GAATGTATGC AAATAAATGC ATACACCATA GGTGTGGTTT AATTTGATGC CCTTTTTCAG 2461 GGCTGGAATG TGTAAGAGCG GGGTTATTTA TGCTGTTGTT TTTTTGTTAC TCGGGAAGGG 2521 CTTTACCTCT TCCGCATAAA CGCTTCCATC AGCGTTTATA GTTAAAAAAA TCTTTCGGCC 2581 TGCATGAATG GCCTTGTTGA TCGCGCTTTG ATATACGCCG AGATCTTTAG CTGTCTTGGT 2641 TTGCCCAAAG CGCATTGCAT AATCTTTCAG GGTTATGCGT TGTTCCATAC AACCTCCTTA 2701 GTACATGCAA CCATTATCAC CGCCAGAGGT AAAATAGTCA ACACGCACGG TGTTAGATAT 2761 TTATCCCTTG CGGTGATAGA TTTAACGTAT GAGCACAAAA AAGAAACCAT TAACACAAGA 2821 GCAGCTTGAG GACGCACGTC GCCTTAAAGC AATTTATGAA AAAAAGAAAA ATGAACTTGG 2881 CTTATCCCAG GAATCTGTCG CAGACAAGAT GGGGATGGGG CAGTCAGGCG TTGGTGCTTT 2941 ATTTAATGGC ATCAATGCAT TAAATGCTTA TAACGCCGCA TTGCTTACAA AAATTCTCAA 3001 AGTTAGCGTT GAAGAATTTA GCCCTTCAAT CGCCAGAGAA ATCTACGAGA TGTATGAAGC 3061 GGTTAGTATG CAGCCGTCAC TTAGAAGTGA GTATGAGTAC CCTGTTTTTT CTCATGTTCA 3121 GGCAGGGATG TTCTCACCTG AGCTTAGAAC CTTTACCAAA GGTGATGCGG AGAGATGGGT 3181 AAGCACAACC AAAAAAGCCA GTGATTCTGC ATTCTGGCTT GAGGTTGAAG GTAATTCCAT 3241 GACCGCACCA ACAGGCTCCA AGCCAAGCTA GCTTCACGCT GCCGCAAGCA CTCAGGGCGC 3301 AAGGGCTGCT AAAGGAAGCG GAACACGTAG AAAGCCAGTC CGCAGAAACG GTGCTGACCC 3361 CGGATGAATG TCAGCTACTG GGCTATCTGG ACAAGGGAAA ACGCAAGCGC AAAGAGAAAG 3421 CAGGTAGCTT GCAGTGGGCT TACATGGCGA TAGCTAGACT GGGCGGTTTT ATGGACAGCA 3481 AGCGAACCGG AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA 3541 AACTGGATGG CTTTCTTGCC GCCAAGGATC TGATGGCGCA GGGATCAAGA TCTGATCAAG 3601 AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG 3661 CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG 3721 ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC 3781 TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA 3841 CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC 3901 TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG 3961 TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT 4021 TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG 4081 TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA 4141 GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT 4201 TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG 4261 GTGTGGCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 4321 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 4381 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG 4441 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT 4501 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG 4561 GATCTCATGC TGGAGTTCTT CGCCCACCCC GGGCTCGATC CCCTCGCGAG TTGGTTCAGC 4621 TGCTGCCTGA GGCTGGACGA CCTCGCGGAG TTCTACCGGC AGTGCAAATC CGTCGGCATC 4681 CAGGAAACCA GCAGCGGCTA TCCGCGCATC CATGCCCCCG AACTGCAGGA GTGGGGAGGC 4741 ACGATGGCCG CTTTGGTCGG ATCAATTCGC GCGACCGATC GATCCGGGCA ACGTTGTTGC 4801 CATTGCTGCA GGGGGGGGGG GGGGGGGGGG GGGGGGTTGA CTTCCATTGT TCATTCCACG 4861 GACAAAAACA GAGAAAGGAA ACGACAGAGG CCAAAAAGCC TCGCTTTCAG CACCTGTCGT 4921 TTCCTTTCTT TTCAGAGGGT ATTTTAAATA AAAACATTAA GTTATGACGA AGAAGAACGG 4981 AAACGCCTTA AACCGGAAAA TTTTCATAAA TAGCGAAAAC CCGCGAGGTC GCCGCCCGTA 5041 ACCTGTCGGA TCACCGGAAA GGACCCGTAA AGTGATAATG ATTATCATCT ACATATCACA 5101 ACGTGCGTGG AGGCCATCAA ACCACGTCAA ATAATCAATT ATGACGCAGG TATCGT // 1447 LOCUS ADDGENE_1447 3811 bp DNA circular SYN 01/03/2005 DEFINITION pPD49_78 complete plasmid sequence KEYWORDS . SOURCE pPD49_78 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1447 at www.addgene.org FEATURES Location/Qualifiers source 1..3811 /organism="Addgene plasmid pPD49_78" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 528..545 /label="rf21.28" promoter 1725..1753 /label="Amp_promoter" CDS 1795..2655 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1795..2655 /label="Ampicillin" /gene="Ampicillin" rep_origin 2810..3429 /label="pBR322_origin" misc_feature 3645..3671 /label="lacI.C" promoter 3738..3767 /label="lac_promoter" misc_feature 3802..3820 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGCTAGCGT 541 CGACGGTACC ATGGTATTGA TATCTGAGCT CCGCATCGGC CGCTGTCATC AGATCGCCAT 601 CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT 661 TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT 721 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA 781 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT 841 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT 901 TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG 961 TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT CAAATCATGC 1021 TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA AGTTTATGAA 1081 ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG ATTTCGAGGA 1141 CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA AGATCGGAAG 1201 AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG AAAGTGGAGT 1261 AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC CAAACATAAC 1321 TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG 1381 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 1441 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA 1501 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 1561 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT GATACGCCTA 1621 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 1681 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 1741 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 1801 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 1861 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 1921 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 1981 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 2041 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 2101 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 2161 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 2221 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 2281 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 2341 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 2401 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 2461 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 2521 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 2581 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 2641 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 2701 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 2761 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 2821 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 2881 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 2941 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 3001 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 3061 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 3121 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 3181 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC 3241 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 3301 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 3361 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 3421 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 3481 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 3541 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 3601 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 3661 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 3721 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 3781 AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1448 LOCUS ADDGENE_1448 3818 bp DNA circular SYN 01/03/2005 DEFINITION pPD49_83 complete plasmid sequence KEYWORDS . SOURCE pPD49_83 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1448 at www.addgene.org FEATURES Location/Qualifiers source 1..3818 /organism="Addgene plasmid pPD49_83" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 535..552 /label="rf21.28" promoter 1732..1760 /label="Amp_promoter" gene 1802..2662 /label="Ampicillin" /gene="Ampicillin" CDS 1802..2662 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2817..3436 /label="pBR322_origin" misc_feature 3652..3678 /label="lacI.C" promoter 3745..3774 /label="lac_promoter" misc_feature 3809..3827 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGCGTCGA CGGTACCATG GTATTGATAT CTGAGCTCCG CATCGGCCGC TGTCATCAGA 601 TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA 661 TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA 721 TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA 781 AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA 841 TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG 901 ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC 961 GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA 1021 ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT 1081 TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT 1141 TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA 1201 TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA 1261 GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA 1321 ACATAACTGT TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT 1381 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG 1441 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC 1501 TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA 1561 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT 1621 ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC 1681 TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT 1741 GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG 1801 TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC 1861 TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC 1921 ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC 1981 CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC 2041 CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT 2101 GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT 2161 ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT 2221 CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT 2281 TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT 2341 GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC 2401 TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG 2461 CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC 2521 TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA 2581 CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC 2641 CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA 2701 TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT 2761 GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT 2821 CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA 2881 ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA 2941 GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT 3001 AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT 3061 ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA 3121 GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT 3181 GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC 3241 GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA 3301 GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG 3361 CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA 3421 AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT 3481 GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC 3541 TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA 3601 AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG 3661 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA 3721 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG 3781 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCT // 1449 LOCUS ADDGENE_1449 7017 bp DNA circular SYN 01/03/2005 DEFINITION pPD50_14 complete plasmid sequence KEYWORDS . SOURCE pPD50_14 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1449 at www.addgene.org FEATURES Location/Qualifiers source 1..7017 /organism="Addgene plasmid pPD50_14" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..3731 /label="lacZ" /gene="lacZ" /note="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 684..3728 /label="lacZ" /gene="lacZ" promoter 4931..4959 /label="Amp_promoter" gene 5001..5861 /label="Ampicillin" /gene="Ampicillin" CDS 5001..5861 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6016..6635 /label="pBR322_origin" misc_feature 6851..6877 /label="lacI.C" promoter 6944..6973 /label="lac_promoter" misc_feature 7008..7026 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg 601 tgggtgaaga ccagaaacag cacctcgaac tgagccgcga tattgcccag cgtttcaacg 661 cgctgtatgg cgagatcgat cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 721 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 781 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg 841 cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg 901 ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg CCCATCTACA 961 CCAACGTGAC CTATCCCATT ACGgtcaatc cgccgtttgt tcccacggag aatccgacgg 1021 gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa 1081 ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt 1141 acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag 1201 aaaaccgcct cgcGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTatctg gaagatcagg 1261 atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac 1321 aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg 1381 aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat 1441 ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg 1501 agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt 1561 ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca 1621 cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc 1681 tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc 1741 ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc 1801 agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc 1861 tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg 1921 tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa 1981 cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg 2041 aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt 2101 cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc 2161 cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca 2221 aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg 2281 cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc 2341 gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa 2401 acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt 2461 tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa 2521 aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg 2581 aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta 2641 agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg 2701 aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag 2761 tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc 2821 gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga 2881 ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc 2941 agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc 3001 gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca 3061 ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag 3121 cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg 3181 cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta 3241 gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc 3301 ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag 3361 ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc 3421 cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga 3481 cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc 3541 gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag 3601 gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc 3661 cgtcagtatc ggcggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt 3721 gtcaaaaata ataggcctaC TAGCGTCGAC GGTACCATGG TATTGATATC TGAGCTCCGC 3781 ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 3841 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 3901 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 3961 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 4021 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 4081 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 4141 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 4201 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 4261 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 4321 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 4381 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 4441 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 4501 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 4561 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 4621 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 4681 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 4741 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 4801 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 4861 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 4921 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 4981 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 5041 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 5101 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 5161 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 5221 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 5281 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 5341 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 5401 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 5461 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 5521 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 5581 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 5641 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 5701 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 5761 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 5821 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 5881 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 5941 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 6001 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 6061 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 6121 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 6181 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 6241 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 6301 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 6361 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 6421 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 6481 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 6541 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 6601 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 6661 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 6721 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 6781 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 6841 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 6901 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 6961 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1450 LOCUS ADDGENE_1450 7024 bp DNA circular SYN 01/03/2005 DEFINITION pPD50_21 complete plasmid sequence KEYWORDS . SOURCE pPD50_21 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1450 at www.addgene.org FEATURES Location/Qualifiers source 1..7024 /organism="Addgene plasmid pPD50_21" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..3738 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVGEDQKQHLE LSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHP LWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVI IWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPF PAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDW VDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQ FRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELP QPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSE MDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRID PNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDG SGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRW DLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLL HAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 691..3735 /label="lacZ" /gene="lacZ" promoter 4938..4966 /label="Amp_promoter" CDS 5008..5868 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5008..5868 /label="Ampicillin" /gene="Ampicillin" rep_origin 6023..6642 /label="pBR322_origin" misc_feature 6858..6884 /label="lacI.C" promoter 6951..6980 /label="lac_promoter" misc_feature 7015..7033 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaccggtgg gtgaagacca gaaacagcac ctcgaactga gccgcgatat tgcccagcgt 661 ttcaacgcgc tgtatggcga gatcgatccc gtcgttttac aacgtcgtga ctgggaaaac 721 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 781 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 841 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt 901 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgCCC 961 ATCTACACCA ACGTGACCTA TCCCATTACG gtcaatccgc cgtttgttcc cacggagaat 1021 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag 1081 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg 1141 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc 1201 gccggagaaa accgcctcgc GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTatctggaa 1261 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg 1321 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct 1381 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt 1441 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt 1501 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg 1561 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc 1621 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa 1681 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag 1741 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg 1801 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg 1861 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac 1921 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa 1981 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg 2041 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc 2101 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt 2161 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg 2221 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac 2281 gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag 2341 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat 2401 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat 2461 cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg 2521 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg 2581 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg 2641 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag 2701 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta 2761 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag 2821 cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg 2881 catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt 2941 aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg 3001 ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg 3061 acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc 3121 gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc 3181 gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt 3241 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat 3301 ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc 3361 ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg 3421 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc 3481 tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac 3541 atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg 3601 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc 3661 tggagcccgt cagtatcggc ggaattccaa ctgagcgccg gtcgctacca ttaccaactt 3721 gtctggtgtc aaaaataata ggcctaCTAG CGTCGACGGT ACCATGGTAT TGATATCTGA 3781 GCTCCGCATC GGCCGCTGTC ATCAGATCGC CATCTCGCGC CCGTGCCTCT GACTTCTAAG 3841 TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT 3901 TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC 3961 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA 4021 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG 4081 TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC 4141 ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG 4201 CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT 4261 TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG 4321 GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG 4381 TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA 4441 GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT 4501 GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC 4561 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 4621 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 4681 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC 4741 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 4801 TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 4861 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 4921 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 4981 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 5041 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 5101 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 5161 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 5221 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 5281 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 5341 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 5401 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 5461 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 5521 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 5581 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 5641 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 5701 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 5761 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 5821 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 5881 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 5941 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 6001 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 6061 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 6121 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 6181 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 6241 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 6301 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 6361 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 6421 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 6481 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 6541 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 6601 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 6661 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 6721 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 6781 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 6841 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 6901 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 6961 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 7021 AGCT // 1451 LOCUS ADDGENE_1451 4263 bp DNA circular SYN 01/03/2005 DEFINITION pPD52_102 complete plasmid sequence KEYWORDS . SOURCE pPD52_102 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1451 at www.addgene.org FEATURES Location/Qualifiers source 1..4263 /organism="Addgene plasmid pPD52_102" /mol_type="other DNA" misc_feature 48..70 /label="mec7.-836" misc_feature 860..883 /label="mec7.Tsa" misc_feature 932..973 /label="SynIVS.A" misc_feature 980..997 /label="rf21.28" promoter 2177..2205 /label="Amp_promoter" gene 2247..3107 /label="Ampicillin" /gene="Ampicillin" CDS 2247..3107 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3262..3881 /label="pBR322_origin" misc_feature 4097..4123 /label="lacI.C" promoter 4190..4219 /label="lac_promoter" misc_feature 4254..4272 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTctagAAAT GTAAACCTGT 61 CATTTCTGTG TATTTCAGCA CCGCGAAGAG CACCATAAAC AGCTACAACA AGAGCACGAA 121 CGTCGGCTCC AACAGAAAAA GCTGCAAAAC GCTGGTACTC ACCACATGCT CTGATAATTG 181 CCATTTCCTT CGATTTCTGA CTTTCAATTG TGATATGATA ACCTCAAAAT CTCCATTCAA 241 AATGAAAACT CTTAAATTTA AGAGCTTTCA ACACCCCGCA CTTTCCCACC TCATCGTTTT 301 TCCCTTCTAT CTTATCAATT TTTTGTATTT TGTGTCAGTT TGCTATATCC TCATCACCTT 361 TTTTACCGTT TAATCTCTCT ATAGTATTGT TCGGGTGTTT CAAGATGAAA CGTTTTGTGT 421 GTAGCATTTT TGAAACACAG CGCGAAAAAT GCAGAAACTA TCATCCACAA TGCATTAAGT 481 GCACTCATTG TGATGACTCC TAACCCCCTC GCCCCACCAG TTGTCTCTTC ACAAATTCAT 541 GGCACAATTC AAAAATGGCT TTTTGTGTTG GATCTTGATC TCGTCTCTTT CCTTCTTCTT 601 TCGTTTCTCT CGGACCACTC ATTATTCGAA TGCCATGCAT TTGTTGATGA TGCGCAGCGA 661 TGGTCGCACA CACTACAACA GATATGATGG TGGCTGTGCG TGGTGCAGAG CTCATGAATA 721 AGTGATGGGG CACCGAGATG TGACTCCTCC CATTATTCGT CTTGAGTCGC ATCTTTTGTC 781 TTGGTCCGTT GTCAGTCGAG CCTCAAGGCA GCCGTTCCGC CAGTGATCGC CTGCTAAGAA 841 GTTGTAGGTG TAGAGGAAGA AACGTCGGGT CCAAATTTCA AGcaacatcc gcgagatcgt 901 gaatcccgGG GATTGGCCAA AGGACCCAAA GGTATGTTTC GAATGATACT AACATAACAT 961 AGAACATTTT CAGGAGGACC CTTGGCTAGC GTCGACGGTA CCATGGTATT GATATCTGAG 1021 CTCCGCATCG GCCGCTGTCA TCAGATCGCC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT 1081 CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT 1141 GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT 1201 AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA 1261 TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT 1321 ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA 1381 CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC 1441 ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT 1501 TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG 1561 GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT 1621 ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG 1681 GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG 1741 ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC 1801 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 1861 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 1921 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 1981 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 2041 CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 2101 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 2161 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 2221 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 2281 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 2341 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 2401 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 2461 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 2521 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 2581 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 2641 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 2701 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 2761 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 2821 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 2881 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 2941 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 3001 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 3061 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 3121 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 3181 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 3241 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 3301 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 3361 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 3421 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 3481 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 3541 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 3601 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 3661 AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 3721 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 3781 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 3841 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 3901 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 3961 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 4021 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 4081 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 4141 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 4201 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 4261 GCT // 1452 LOCUS ADDGENE_1452 5297 bp DNA circular SYN 01/03/2005 DEFINITION pPD57_56 complete plasmid sequence KEYWORDS . SOURCE pPD57_56 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1452 at www.addgene.org FEATURES Location/Qualifiers source 1..5297 /organism="Addgene plasmid pPD57_56" /mol_type="other DNA" misc_feature 29..52 /label="mec3.-1822" misc_feature 1851..1874 /label="mec3.Tsa" misc_feature 1966..2007 /label="SynIVS.A" misc_feature 2014..2031 /label="rf21.28" promoter 3211..3239 /label="Amp_promoter" gene 3281..4141 /label="Ampicillin" /gene="Ampicillin" CDS 3281..4141 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4296..4915 /label="pBR322_origin" misc_feature 5131..5157 /label="lacI.C" promoter 5224..5253 /label="lac_promoter" misc_feature 5288..5306 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTACC CGGAGTAGTT GGCGAAGATA 61 GAAATGGTGT TATCTTATAT AAATTCAGAC TTTCTCTGTT AAAATTTTAA CCACCAAAAA 121 ATATAGAATT TATAAAAAGT TTCATTTTGA AATTTTATCT TTTGGGGCCA TTTCACTACC 181 TTATCAGGAA GAAATGTGTT TCATCAGAAA TTGAATTTTT CATCTTTTCA GAAAGTGTAC 241 CTAATACCGT ATCATGCTCA TATTCACGTG AATAAGGCTT CTGCCCAGTT CTGTGATTCA 301 AGGAAAACTA CGAAAAATGA TCTCATTTTC TTTGATTTAT CACCGAGCTG GTGTGTCATC 361 ACACACAGTC TGTTTCTTGA TAAAAACGCG CAGAAGTTAG AGCATTCATT GTGATTTTGG 421 GTCTTTAATT TCTGTAAAAC AAATAAAGTG ACAAGTTTAT CATCTGCCTA TTTTATCAGT 481 TATATGCATG GTTAGTTGCA AATAAGGGAG TAGAAATTGA CTAAAACTCA AAATGAGCAC 541 TGAAACGAGG GGTTTTGTTA GTTGCGAAAT AACAATTAAT ACAATCTGGT TGATTACTTT 601 GTAAAAGGAT AATAAAATAT GCAAAACTTG GCGTATTCTG AGATTTCATT CCTAAAGACA 661 TTTATCAAAA CAAATGTTTT CTGCAATTTT CTAAATTCTT GGAATTCTGC CCAGCAGCCT 721 CTAATATCGC GTTTTACTTA GAAAATGACT TTTGGTTTCA TTTTCTCATT TCTGGCTTCT 781 ATTTTTCACA AATGCAAATA TTACATTTAA ATTAGTACTT TCATTTCTAC AAAGATCAAA 841 TTTAACATTA TTGACAGGTT TACTGTTATC TTGTGACAGT TCCGTACTGT CAATCAAAAC 901 GAAAATTAAA ACTCATGAAA TATCAATGAA CATAAATTGA AAAAAAAACC CAGTTGAAAG 961 GAGAAAATAA GCGAAACACA CCAACAACTG AGTAGTTACG CTCACAATTT GGAAATGATT 1021 TTGAAAACTC TCTGGACTGC ATTTCAATTT TTAACAAACA ATGAATACAT ACTGATAGTT 1081 TTTGTACTTC TCGGCATTCA GAGGCAACTT CCTGCCAACA ACCTGGCAAC TTATTTAGTG 1141 CCACTAAAAT AAGTTGCCAA ATTGTTGGCA GGAAGTTGCC TCCAAATGCC GAGTTGGAGC 1201 AAATGATTCG ATATGATTTT AAGGATACCT ACGCATGCCT CCTGCCAAGA CACGCACCCA 1261 CCTACTGAAA TGTCGCGGAA TTTTACCTCC CAAACTATAG ATTGGGTGAA ACTTCACTGT 1321 AAAAGCTTTT TTTAAGTAGT AGGCTGGCAG TAATGAAGAC GTCCATACCA TAATTTTTAA 1381 TGTTGTATAG AAAATCTGAA TTCTCAAGTA TAACATTTTC CAATAATTAG CATAGTCAGA 1441 AACTCTCCAC CGCCGTTCCG TTCCTTTTTT TTGTAACTAT GGAGTAATAG AACAAGATTA 1501 TTCAAATTCA AATTTTTATA AAACTTTACA ACGTCCTTTC CATATATTCT TCACACAACA 1561 TTTGAAAAAA CAACAAATTC ATTCGAAATG CATTGCCCAT AATGAATCGA CCGAAAAACA 1621 CAAGTGACCG TCAGGAGATC GATAGAGAGA GCCCGTTCCA ACTAGACAAC TTTTTAGTGC 1681 TTATCCTTAC ACACACTTTC TAGCTTCATA AGAAATGCAT CTATTATCGT CACCATTTGG 1741 AGACACCAGT TTTAGCGCAC ATTAATAATC GATCAGGGTC TAGAGACTCC TGTTGGATTG 1801 GCATGCCACG CCTACATGAC ATCTGGCTCT TGACGGGTGA TTTGTTACAG TGAGTAGCCA 1861 CAATTTCGCG CATTTTCTTC TAAATTTCAC TGTTATTTGT TCTTctgatt tatttcattt 1921 gaactacgga aatcgGATCC CCGGGATTGG CCAAAGGACC CAAAGGTATG TTTCGAATGA 1981 TACTAACATA ACATAGAACA TTTTCAGGAG GACCCTTGGC TAGCGTCGAC GGTACCATGG 2041 TATTGATATC TGAGCTCCGC ATCGGCCGCT GTCATCAGAT CGCCATCTCG CGCCCGTGCC 2101 TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC 2161 CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT 2221 TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA 2281 AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC 2341 ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA 2401 TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT 2461 TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG 2521 TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC 2581 TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG 2641 CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG 2701 AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT 2761 TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT 2821 CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC 2881 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC 2941 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA 3001 GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 3061 AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA 3121 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 3181 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 3241 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 3301 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 3361 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 3421 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 3481 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 3541 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 3601 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 3661 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 3721 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 3781 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 3841 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 3901 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 3961 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 4021 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 4081 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 4141 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 4201 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 4261 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 4321 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 4381 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 4441 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 4501 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 4561 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 4621 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 4681 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 4741 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 4801 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 4861 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4921 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC 4981 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG 5041 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC 5101 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 5161 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA 5221 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT 5281 TCACACAGGA AACAGCT // 1453 LOCUS ADDGENE_1453 11960 bp DNA circular SYN 01/03/2005 DEFINITION pPD5_41 complete plasmid sequence KEYWORDS . SOURCE pPD5_41 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1453 at www.addgene.org FEATURES Location/Qualifiers source 1..11960 /organism="Addgene plasmid pPD5_41" /mol_type="other DNA" gene 612..7881 /label="unc54(w/ gaps)" /gene="unc54(w/ gaps)" misc_feature 1696..1726 /label="unc54.enh" CDS complement(2515..3249) /label="Orf0" /translation="MVEELLELLVYEVNPQLLEGVEVEDFETGDIEHTDEIITINTLL IQSEVTLLDKPVEHTGVETLGHGSDSPIDLFAVLSLGYPFGSDLDTWLGQSLEELLAS DSVHVGCLLGLYGTIWLSLLFTWALLELHVTHVHVSGHEAVAILLLDGREVEDVESFI SKLELFDVIDSVNDQLGLGDEPVILDWQVKKKFLLELWTEVRVDLVEDVVATLSWSLT DDTRFLEQVCKIYSLNNSCIIFIELC" CDS 3426..7091 /label="Orf2" /translation="MLDEECIVPKATDLTLASKLVDQHLGKHPNFEKPKPPKGKQGEA HFAMRHYAGTVRYNCLNWLEKNKDPLNDTVVSAMKQSKGNDLLVEIWQDYTTQEEAAA KAKEGGGGGKKKGKSGSFMTVSMLYRESLNNLMTMLNKTHPHFIRCIIPNEKKQSGMI DAALVLNQLTCNGVLEGIRICRKGFPNRTLHPDFVQRYAILAAKEAKSDDDKKKCAEA IMSKLVNDGSLSEEMFRIGLTKVFFKAGVLAHLEDIRDEKLATILTGFQSQIRWHLGL KDRKRRMEQRAGLLIVQRNVRSWCTLRTWEWFKLYGKVKPMLKAGKEAEELEKINDKV KALEDSLAKEEKLRKELEESSAKLVEEKTSLFTNLESTKTQLSDAEERLAKLEAQQKD ASKQLSELNDQLADNEDRTADVQRAKKKIEAEVEALKKQIQDLEMSLRKAESEKQSKD HQIRSLQDEMQQQDEAIAKLNKEKKHQEEINRKLMEDLQSEEDKGNHQNKVKAKLEQT LDDLEDSLEREKRARADLDKQKRKVEGELKIAQENIDESGRQRHDLENNLKKKESELH SVSSRLEDEQALVSKLQRQIKDGQSRISELEEELENERQSRSKADRAKSDLQRELEEL GEKLDEQGGATAAQVEVNKKREAELAKLRRDLEEANMNHENQLGGLRKKHTDAVAELT DQLDQLNKAKAKVEKDKAQAVRDAEDLAAQLDQETSGKLNNEKLAKQFELQLTELQSK ADEQSRQLQDFTSLKGRLHSENGDLVRQLEDAESQVNQLTRLKSQLTSQLEEARRTAD EEARERQTVAAQAKNYQHEAEQLQESLEEEIEGKNEILRQLSKANADIQQWKARFEGE GLLKADELEDAKRRQAQKINELQEALDAANSKNASLEKTKSRLVGDLDDAQVDVERAN GVASALEKKQKGFDKIIDEWRKKTDDLAAELDGAQRDLRNTSTDLFKAKNAQEELAEV VEGLRRENKSLSQEIKDLTDQLGEGGRSVHEMQKIIRRLEIEKEELQHALDEAEAALE AEESKVLRAQVEVSQIRSEIEKRIQEKEEEFENTRKNHARALESMQASLETEAKGKAE LLRIKKKLEGDINELEIALDHANKANADAQKNLKRYQEQVRELQLQVEEEQRNGADTR EQFFNAEKRATLLQSEKEELLVANEAAERARKQAEYEAADARDQANEANAQVSSLTSA KRKLEGEIQAIHVSFRY" CDS 3907..4710 /label="Orf0" /translation="MPLWFSTSLPATECWKESEFAERDSPTEPFIQTSSNVTPSLPPR RPSPMTTRRSAPRLSCPSSSTTDPSARRCSVSVSPRSSSRLEFLLILKTSVTRSSPPS SPDSNPKSDGIWVSRTASAVWNNVPDFSLFSATSVPGALSVPGNGSSFTERSSQCSRP ERKPRSSRRSTTRLRPSKTVSPRRRSFARSSRSPLPSSSKRKLLSSPTWNPPRLNSPM LRKDLPSLRLNKRMPPSNSPSSTINSLTTKTVPLMFSALRRRSRPRSRL" CDS complement(3390..4916) /label="Orf2" /translation="MVTLILFGLEVLHQLAVDFFLMLLLLVELGNSFILLLHLILKGS DLVILGLLLRLSLAERHFQVLDLLLQSLDLGLDLLLSALNISGTVFVVSELIVELGEL LGGILLLSLKLGKSFLSIGELSLGGFQVGEERSFLFDELGRGLLELLAKLLLLGETVF EGLNLVVDLLELLGFLSGLEHWLDLSVKLEPFPGTESAPGTDVALNNEKSGTLFHTAL AVLETQMPSDLGLESGEDGGELLVTDVFKMSKNSSLEEDLGETDTEHLLAEGSVVDEL GHDSLGALLLVVIGLGLLGGKDGVTLDEVWMKGSVGESLSANSDSFQHSVAGKLVENQ SGIDHTRLLLLVGDDTTDEMWVSLVEHSHQVVERLSVEHRDGHERSGLSLLLSTATSL LGLSGGLFLGGVVLPDLDEKIVSLGLFHCRDNGVVEGVLVLLEPVQAVVTHSSGVVPH GEVSFSLLSLWWLWLLEVWMLAKMLIDKLGSEGQISSLWYDTLFIKHGDNTEWLKNKN" promoter complement(9112..9130) /label="T7_promoter" misc_feature 9135..9290 /label="lacZ_a" misc_feature complement(9138..9154) /label="M13f20_primer" rep_origin 9316..9622 /label="f1_origin" promoter 9818..9846 /label="Amp_promoter" gene 9888..10748 /label="Ampicillin" /gene="Ampicillin" CDS 9888..10748 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 10903..11522 /label="pBR322_origin" misc_feature 11738..11764 /label="lacI.C" promoter 11831..11860 /label="lac_promoter" misc_feature 11895..11913 /label="M13r_primer" promoter 11930..11949 /label="T3_promoter" ORIGIN 1 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTGGAT CCCCCAACTA GGTCGTAAAT 61 GTATTGATTT GCTTAAAATC TGAAGATCTA GTGGTGAACC GTGGAAGATT ATCAAGAGGA 121 GGCTGAAGAT CTGTTTAAGA ACCATTAATC AAACTGGTAT TCTATTTTCA CTGGTTGTAT 181 GTAAACATTC TATCTTATTC CTTTTATCAC TGTTCTGCAC TTTCCTATAA AAAAAGTTGA 241 CCGACCGTAC TCTCTGAATT CATTTTTCCC GATCTTACCA ACTCCCGATC TATCTCTATC 301 CCTGGTTTTT TCTTCGTGCT CCAATGGAAT TCTTGAGACT TCCACTATCT TCTCTGGCAC 361 CCTCCACTAC GCGTAGGCGT CTCTCGCTTC GTGTATTCCC GGGAAGCCGG GTTCCCGTCT 421 CTCCCGCCGC TGCCGCTGCC GCACACAGCT TTACACCTCG TAGAATCCCC AAAGAGGGGC 481 GTGGCTTGCG GGTGCCAACA TCCTCCTGCC GAGGAAGAAG CAGGCACTCA TCACTCGCAT 541 CATCAACCTC GACTAAGGGA GCACCACATT AGTTTTGAGG TAGAGAAACC ATTTGAAAGA 601 AGCGAGAAAT CATGGAGCAC GAGAAGGACC CAGGATGGCA ATATCTCCGC CGTACCAGAG 661 AGCAGGTTTT GGAGGTGAGT ATTTATTTTT TATTTATTTA TTTTTCTTTC ATTTGTCTTT 721 ATTGTTATGC TCATTTCTAT AGAGTAAATC GTATAGTACT CCATAAGTAT CTAATTTTAA 781 CAAAACTGAG CATTTATTTT TGAAATTCTA GATTTTTAAC ATACAACTGA GTTAAAGTCT 841 GCCATTTTAC ACTTATAGAT TTTTTAAATT CAGGTAGAAG ATTTGAGCTT GCCTTGTAAT 901 CCTTGTAATC AATGTTTATT TCTGTTTTCT AATTTTTCAT AGATTTAGCT TCAAGATCAT 961 CTTTCCCTTG AAATCATTGA AATCTTCCTT TCTTAAATTT TTGCATTGCA CTTGGAATTT 1021 TGTGACACAT TGTGCTGCAG TAGTACTGTA TTTTGAGACG GCACATTTAT TTTGACTACA 1081 CCACAAGCTT AACCTTGATT TTACTTTTTG ATTCCATTCC ATTCATGGGT CTAAAGTGTG 1141 AAGCGGTTTA GACCATTTTT CAACACAAGT TGGTATTCTT TAGAAGTGAT ATATTTCTGA 1201 ATTCTGATCT CTTTTGTACC ATTATTGTAA TTTCAGGATC AATCTAAGCC TTATGACTCC 1261 AAGAAGAACG TCTGGATCCC AGATCCAGAA GAGGGATACC TTGCCGGAGA AATCACCGCC 1321 ACCAAGGGAG ACCAGGTCAC CATCGTCACC GCTCGCGAAA TGAGGTTCGT TTTTAAGCTA 1381 ATGATTACGG TTTTCAATGT AGTGTGATTC AGGTCACCCT CAAGAAGGAG TTGGTTCAAG 1441 AAATGAATCC ACCAAAGTTC GAGAAGACCG AAGACATGTC CAACTTGTCT TTCCTCAACG 1501 ACGCCTCTGT CCTCCATAAC TTGCGTTCCC GTTACGCTGC TATGCTCATC TACGTAAGTT 1561 TCATTGAAAC TTAAAGTTAA GCATTGAGAA TCATTCATAT TCTTCTTCTA AATTCCCATA 1621 AAATCCCGAA ACTCCTTCCC TCTATCTTCT TTTTCTTCTC GTTTTCAAAT GTTTCTCTCT 1681 ATCCCATTCT CTCATCAATT GAGTGGGATG AGGCTATCTC TGCCTCTCTT CTGAATCTCT 1741 GAACCATCTT ACATTACACT GTGGATGACG AGCCCCACAG GCTCCCTTGC ATCAGATACT 1801 GCCATTGGGG ATGGCAAAGA AGAGAGAAGG TATTGTGAGG ATATATTTTT CTAAGAAAAA 1861 ACGTTTGAAG AAAAGAAGAT GAAGAAGATC TGCTTGATTC ATTGCACAAG TTAGAAGTAA 1921 CAGGGGTCTA TATTTCGAAG AACTTAAAGG GAATGCAACT GAACATAAAA TTAAACAAAG 1981 GGATTGAATC CTGCAGTATT TAAAAATCGA AACTAGAATG TTTCCTATTT CAGACCTACT 2041 CCGGACTTTT CTGCGTTGTC ATCAACCCAT ACAAGCGTCT CCCAATCTAC ACCGACTCTT 2101 GCGCCCGTAT GTTCATGGGA AAGAGAAAGA CAGAAATGCC ACCACATTTG TTCGCTGTCT 2161 CTGACGAAGC CTACCGTAAC ATGCTCCAAG ACCACGAGAA CCAGTCTATG CTCATTACCG 2221 GAGAATCTGG AGCCGGAAAG ACTGAGAACA CCAAGAAGGT TATTTGCTAC TTCGCCGCTG 2281 TCGGTGCCTC CCAACAGGAA GGTGGAGCTG AAGTTGATCC AAATAAGAAG AAGGTCACCC 2341 TTGAGGACCA AATCGTCCAG ACCAATCCAG TACTTGAAGC CTTCGGTAAC GCCAAGACTG 2401 TCCGTAACAA CAACTCATCC CGTTTCGGAA AGTTCATCCG TATCCACTTC AACAAGCACG 2461 GACGCCTCGC ATCTTGCGAT ATCGAGCATT GTGAGTTACT TTTTGATTTA TATTCTAGCA 2521 TAGCTCAATA AAAATTATGC AACTATTGTT TAAACTATAA ATTTTGCAGA CTTGCTCGAG 2581 AAATCTCGTG TCATCCGTCA AGCTCCAGGA GAGCGTTGCT ACCACATCTT CTACCAAATC 2641 TACTCTGACT TCCGTCCAGA GCTCAAGAAG GAACTTCTTC TTGACTTGCC AATCAAGGAT 2701 TACTGGTTCG TCGCCCAGGC CGAGTTGATC ATTGACGGAA TCGATGACGT CGAAGAGTTC 2761 CAACTTACTG ATGAAGCTTT CGACATCCTC AACTTCTCTG CCGTCGAGAA GCAGGATTGC 2821 TACCGCCTCA TGTCCGCTCA CATGCACATG GGTAACATGA AGTTCAAGCA ACGCCCACGT 2881 GAAGAGCAAG CTGAGCCAGA TGGTACCGTA GAGGCCGAGA AGGCATCCAA CATGTACGGA 2941 ATCGGATGCG AGGAGTTCCT CAAGGCTTTG ACCAAGCCAC GTGTCAAGGT CGGAACCGAA 3001 TGGGTATCCA AGGGACAGAA CTGCGAACAA GTCAATTGGG CTGTCGGAGC CATGGCCAAG 3061 GGTCTCTACT CCCGTGTGTT CAACTGGCTT GTCAAGAAGT GTAACCTCAC TCTGGATCAA 3121 AAGGGTATTG ATCGTGATTA TTTCATCGGT GTGCTCGATA TCGCCGGTTT CGAAATCTTC 3181 GACTTCAACT CCTTCGAGCA GTTGTGGATT AACTTCGTAA ACGAGAAGCT CCAACAATTC 3241 TTCAACCATC ACATGTTCGT CCTTGAACAG GAAGAATACG CCCGTGAGGG TATTCAATGG 3301 GTCTTCATCG ATTTCGGACT TGATTTGCAA GCGTGTATCG AACTTATTGA GAAGGTAAGA 3361 TTGAATTGAA ATTTTAAATG TTATTTTGAT CAATTCTTAT TTTTCAGCCA CTCGGTATTA 3421 TCTCCATGCT TGATGAAGAG TGTATCGTAC CAAAGGCTAC TGATTTGACC CTCGCTTCCA 3481 AGCTTGTCGA TCAACATCTT GGCAAGCATC CAAACTTCGA GAAGCCAAAG CCACCAAAGG 3541 GAAAGCAAGG AGAAGCTCAC TTCGCCATGC GGCACTACGC CGGAACTGTG CGTTACAACT 3601 GCTTGAACTG GCTCGAGAAG AACAAGGACC CCCTCAACGA CACCGTTGTC TCGGCAATGA 3661 AACAATCCAA GGGAAACGAT CTTCTCGTCG AGATCTGGCA AGACTACACC ACCCAGGAAG 3721 AGGCCGCCGC TAAGGCCAAG GAGGGAGGTG GCGGTGGAAA GAAGAAGGGA AAGTCCGGAT 3781 CTTTCATGAC CGTCTCTATG CTCTACAGAG AGTCTCTCAA CAACTTGATG ACTATGCTCA 3841 ACAAGACTCA CCCACATTTC ATCCGTTGTA TCATCCCCAA CGAGAAGAAG CAATCTGGTA 3901 TGATCGATGC CGCTTTGGTT CTCAACCAGC TTACCTGCAA CGGAGTGTTG GAAGGAATCA 3961 GAATTTGCAG AAAGGGATTC CCCAACAGAA CCCTTCATCC AGACTTCGTC CAACGTTACG 4021 CCATCCTTGC CGCCAAGGAG GCCAAGTCCG ATGACGACAA GAAGAAGTGC GCCGAGGCTA 4081 TCATGTCCAA GCTCGTCAAC GACGGATCCC TCAGCGAGGA GATGTTCCGT ATCGGTCTCA 4141 CCAAGGTCTT CTTCAAGGCT GGAGTTCTTG CTCATCTTGA AGACATCCGT GACGAGAAGC 4201 TCGCCACCAT CCTCACCGGA TTCCAATCCC AAATCAGATG GCATTTGGGT CTCAAGGACC 4261 GCAAGCGCCG TATGGAACAA CGTGCCGGAC TTCTCATTGT TCAGCGCAAC GTCCGTTCCT 4321 GGTGCACTCT CCGTACCTGG GAATGGTTCA AGCTTTACGG AAAGGTCAAG CCAATGCTCA 4381 AGGCCGGAAA GGAAGCCGAG GAGCTCGAGA AGATCAACGA CAAGGTTAAG GCCCTCGAAG 4441 ACAGTCTCGC CAAGGAGGAG AAGCTTCGCA AGGAGCTCGA GGAGTCCTCT GCCAAGCTCG 4501 TCGAAGAGAA AACTTCTCTC TTCACCAACT TGGAATCCAC CAAGACTCAA CTCTCCGATG 4561 CTGAGGAAAG ACTTGCCAAG CTTGAGGCTC AACAAAAGGA TGCCTCCAAG CAACTCTCCG 4621 AGCTCAACGA TCAACTCGCT GACAACGAAG ACCGTACCGC TGATGTTCAG CGCGCTAAGA 4681 AGAAGATCGA GGCCGAGGTC GAGGCTTTGA AGAAGCAGAT CCAAGACTTG GAAATGTCTC 4741 TCCGCAAGGC TGAGTCTGAG AAGCAATCCA AGGATCACCA GATCAGATCC CTTCAAGATG 4801 AGATGCAACA GCAAGATGAA GCTATTGCCA AGCTCAACAA GGAGAAGAAG CATCAAGAAG 4861 AAATCAACCG CAAGCTGATG GAGGACCTCC AATCCGAAGA GGATAAGGGT AACCATCAGA 4921 ACAAGGTCAA GGCCAAGCTT GAGCAGACTC TTGATGACCT CGAGGATTCC CTTGAGCGCG 4981 AGAAGAGAGC CCGCGCCGAT CTTGACAAGC AGAAGAGAAA GGTCGAGGGA GAGCTCAAGA 5041 TTGCTCAAGA GAACATCGAT GAGAGCGGAC GCCAACGCCA CGATCTTGAG AACAACTTGA 5101 AGAAGAAGGA ATCTGAGTTG CACTCAGTTT CTTCCCGACT TGAGGACGAA CAAGCTCTTG 5161 TCTCCAAGCT CCAACGCCAG ATCAAGGACG GACAAAGCCG TATCTCCGAG CTCGAAGAGG 5221 AACTCGAGAA TGAACGTCAA TCCCGTTCCA AGGCTGACCG TGCCAAGAGC GACCTCCAAC 5281 GCGAGTTGGA AGAGCTCGGT GAGAAGCTTG ACGAGCAAGG TGGAGCTACT GCCGCCCAGG 5341 TTGAGGTCAA CAAGAAGCGT GAGGCTGAAC TTGCCAAGCT CCGCAGAGAC TTGGAGGAGG 5401 CCAACATGAA CCACGAGAAC CAACTCGGTG GACTTCGCAA GAAGCACACC GACGCTGTCG 5461 CTGAGCTCAC CGACCAACTC GATCAACTCA ACAAGGCCAA GGCTAAGGTC GAGAAGGACA 5521 AGGCTCAAGC TGTTCGTGAC GCTGAGGACC TTGCTGCTCA ACTTGACCAA GAAACCTCTG 5581 GAAAGCTCAA CAACGAAAAG CTTGCCAAGC AGTTCGAACT TCAACTCACC GAGCTTCAAT 5641 CTAAGGCTGA TGAGCAATCT CGCCAACTCC AAGACTTCAC TTCCCTTAAG GGACGCCTTC 5701 ACTCTGAGAA TGGAGACCTT GTCCGTCAAC TCGAAGATGC CGAATCCCAA GTCAACCAAC 5761 TCACCAGACT GAAGTCCCAG CTTACTTCTC AACTCGAGGA GGCTCGCCGC ACCGCTGACG 5821 AGGAAGCACG CGAACGTCAA ACCGTCGCCG CCCAGGCTAA GAACTACCAA CACGAAGCCG 5881 AGCAACTCCA AGAGTCCCTT GAAGAGGAGA TCGAAGGAAA GAACGAGATC CTCAGACAAC 5941 TCTCCAAGGC TAACGCCGAC ATCCAACAAT GGAAGGCTCG CTTCGAGGGA GAAGGACTCC 6001 TCAAGGCCGA CGAGCTCGAG GATGCCAAGA GACGCCAAGC CCAAAAGATC AACGAGCTCC 6061 AAGAGGCTCT TGATGCCGCC AACTCTAAGA ACGCTTCTCT TGAGAAGACC AAGTCCAGAC 6121 TTGTCGGAGA CTTGGACGAC GCTCAAGTTG ATGTCGAGAG AGCCAACGGT GTCGCCAGTG 6181 CTCTTGAGAA GAAGCAAAAG GGCTTCGACA AGATCATCGA CGAATGGAGA AAGAAGACCG 6241 ACGACTTGGC CGCTGAGCTT GATGGAGCTC AACGCGACCT TCGCAACACC TCCACTGATC 6301 TCTTCAAGGC CAAGAACGCC CAAGAGGAGC TCGCCGAGGT TGTTGAGGGA CTCCGCCGTG 6361 AGAACAAGAG CTTGAGCCAA GAGATCAAGG ATCTTACCGA TCAACTCGGA GAGGGAGGAC 6421 GCTCTGTCCA CGAAATGCAA AAGATCATCC GCCGTCTTGA GATTGAGAAG GAAGAACTCC 6481 AACACGCTTT GGACGAGGCT GAGGCTGCCC TTGAAGCTGA AGAGAGCAAG GTTCTCCGCG 6541 CCCAGGTTGA AGTTTCCCAG ATCCGTTCCG AAATCGAGAA ACGCATCCAG GAGAAGGAGG 6601 AAGAGTTCGA GAACACGAGA AAGAACCACG CCCGCGCTCT TGAATCAATG CAAGCTTCCC 6661 TCGAGACCGA AGCTAAAGGA AAGGCCGAAC TTCTCCGCAT CAAGAAGAAG CTCGAGGGAG 6721 ATATCAACGA GCTCGAGATC GCTTTGGACC ACGCCAACAA GGCTAACGCC GATGCCCAGA 6781 AGAACTTGAA GAGATACCAA GAGCAAGTCC GCGAGTTGCA ATTGCAAGTC GAGGAGGAGC 6841 AACGCAATGG AGCCGACACC CGTGAACAAT TCTTCAACGC CGAGAAGCGC GCCACTCTTC 6901 TTCAATCCGA GAAGGAAGAA CTTTTGGTTG CCAACGAGGC CGCCGAGAGA GCCCGCAAGC 6961 AAGCCGAGTA TGAAGCCGCC GATGCTCGTG ATCAAGCCAA CGAAGCCAAC GCTCAAGTCA 7021 GCAGCTTGAC TTCTGCCAAG AGAAAGCTCG AGGGAGAAAT CCAAGCCATT CATGTAAGTT 7081 TTAGATATTG ACAATCTAGT TTTTCAGTCT ATTCAATGTG AATCTTTCAG GCCGACCTCG 7141 ATGAGACCCT CAACGAGTAC AAGGCCGCTG AGGAGCGCTC CAAGAAGGCT ATTGCTGATG 7201 CCACCAGACT CGCAGAGGAG CTCCGTCAAG AACAAGAACA CTCTCAGCAC GTTGACCGTC 7261 TTCGCAAGGG ACTTGAGCAA CAGCTCAAGG AGATCCAAGT CCGTCTTGAT GAGGCCGAGG 7321 CTGCTGCTCT TAAGGGAGGA AAGAAGGTAA TACATCATAT CGTTCAAAAT ATATACAAAT 7381 TTCATGGTTT TCAGGTTATC GCCAAGCTCG AACAACGCGT GCGTGAGCTC GAATCTGAAT 7441 TGGACGGAGA ACAACGCCGC TTCCAGGATG CCAACAAGAA CCTCGGACGC GCCGATAGAC 7501 GTGTTCGTGA GCTTCAATTC CAGGTTGATG AGGACAAGAA GAACTTCGAA CGTCTCCAAG 7561 ATCTCATTGA CAAGCTCCAA CAGAAGTTGA AGACCCAGAA GAAGCAGGTT GAGGAAGCTG 7621 TAAGTTTATT ATATCTTGTA ACCTGACATT CCATCATCTT ATTAATTTCA GGAGGAACTC 7681 GCCAACTTGA ACCTCCAGAA GTACAAGCAA CTCACCCACC AACTTGAAGA TGCTGAGGAG 7741 CGCGCCGATC AAGCCGAAAA CTCCCTCTCA AAGATGCGAT CAAAATCCCG TGCATCCGCC 7801 TCTGTTGCCC CAGGACTCCA GAGCTCCGCA TCGGCCGCTG TCATCAGATC GCCATCTCGC 7861 GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC 7921 CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT 7981 TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT 8041 TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC 8101 AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT 8161 GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT 8221 GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC 8281 GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA 8341 TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG 8401 TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT 8461 TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT 8521 CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT 8581 AAAATTAAAC ATTTTTCTAA ATTTTATATG ATTTCTTTTA AATTTGCAAA AATTACTTAA 8641 ATTTGAATTC CCGCGCAAAT GAGTGACTTC ATTTTCTGCA TTATTGTGTT TTCCGGCTAT 8701 ATTAATAGGT ATTTGTTTGT GTTTTTCTTT ATTTTATGAT TCGAACTCCA ATTTGTAAAT 8761 TTTCGAACAT ATTTCCCTAA AGAAAAAATA TGATTAATCT GGAAAAATTG GAAAATTATT 8821 TTTCAAATAA AAAACAAAGA AAAAAATGAA GAAAAACCTA TTAGTTTGGC CATAAAACGC 8881 AAAAATGTCG AAAATGACGT CACTCATCTG CGCGGGAAAT CAAGAATAAT TCGGCCTTTT 8941 TTATTTTTTT GGAAAATCGT AAAACATTTA GAAAAATTTT TTAATAGTTA TATGGGACTT 9001 ATTCTGCATT TAGGGCAAAA GCCAGAGACT ACTCCACCGT TGGGCTGCAG GAATTCGATA 9061 TCAAGCTTAT CGATACCGTC GACCTCGAGG GGGGGCCCGG TACCCAATTC GCCCTATAGT 9121 GAGTCGTATT ACAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC 9181 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA 9241 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCGAC 9301 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 9361 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 9421 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 9481 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 9541 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 9601 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 9661 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 9721 GCGAATTTTA ACAAAATATT AACGTTTACA ATTTCCCAGG TGGCACTTTT CGGGGAAATG 9781 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 9841 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 9901 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 9961 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 10021 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC 10081 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG 10141 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 10201 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 10261 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 10321 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC 10381 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 10441 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 10501 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 10561 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 10621 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC 10681 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 10741 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT 10801 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT 10861 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 10921 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 10981 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 11041 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 11101 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 11161 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 11221 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 11281 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA 11341 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 11401 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 11461 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 11521 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 11581 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 11641 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC 11701 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 11761 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG 11821 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT 11881 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTCGGA ATTAACCCTC 11941 ACTAAAGGGA ACAAAAGCTG // 1454 LOCUS ADDGENE_1454 8217 bp DNA circular SYN 01/03/2005 DEFINITION pPD69_39 complete plasmid sequence KEYWORDS . SOURCE pPD69_39 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1454 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid pPD69_39" /mol_type="other DNA" misc_feature 42..66 /label="glp1.delta-1635" misc_feature 1651..1678 /label="glp1.Tsa" CDS 1763..4927 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 1763..1789 /label="SV40.NLS" gene 1880..4924 /label="lacZ" /gene="lacZ" promoter 6131..6159 /label="Amp_promoter" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGCCTT GGTCGAGTAT GAGAAACAAC 61 ATTAACAATG TTTTGGGAGA AACATGTAGT TTTGTACGTC CGGTCCAGTG AGAGTTTTCA 121 ACTTATTTTG GAAGAAATTT TTTTTTGACA AAATTTTGCA ATTTCCCACG CAGAAATACG 181 GTATGCCCCG TATCTCGCCA CGAACATAAT ATTTTAAATT GACGGAAAGG TGTGCGCCTT 241 TAAGAAGCAC AGTAACCATT TTCCGGTTTT TTTTTTGAAA TTGCCTTTGA TTTTTACTTT 301 TTTCTCCCAA AAACGAGAAA TTTAATATAA TCCATCAAAA TCTTTGTGAA CGAGAGAAAT 361 ATAGTGAAAA ACTCAAAGAA AATTGCGAAA TGGATTACTG TACTCCATAA AGGCGCACAT 421 CTTTTCGAAA AATCGTGAAA AATTGCATCT GACCAACAGT TTTGCGTGGA ATCTTCCTAG 481 ATTCAAATTT CCTATGAGTC ATAAAACTAT CATTTTATCG GACAATAACT ACACGTGTAC 541 CTTGTTTCCA TATGTTCTAC CAATATAATT TGTGTTCATA TGTGGCCAGG TACGGGAAAT 601 TAGAACACGG ACTGATTTAG GAAGGTTCAA ATTCGAGAAC AATGGACGAC TTGCTGCACG 661 AAAACGTGGA TTATTCTGAA ACCGCCTATG TGATCACTAT CGATTTAGCA CTTCGGTTTT 721 CTTTTTCCGT TTTCCTTTAT ATTCCGTATT GTATGCCGAC AATAACGCTT TTTCTTTGTA 781 TAAACATTTC CAGAAGCAGA GCAAATTTGT TTATTCCAGA ATTGACTGAA TATTGAAATA 841 ATGTTTACAG ACATACTGTA GATTTTTTTC TATTCAAACA TGAATACAGT TTTTCGTATT 901 TTTAATCTTC AGATAAGTTT TGTGTGTGTT TTTGAGAATA ATATTTAAAA AAGATAAGCA 961 AATATCAGAA CTACGGTAAG CGAAGGGGTA CGGTCGTCAA TTGCGGACTT CCGAGCTGTG 1021 CACGGCGCCA AAATTTATTT TGAGTTTTAA AATGATTTTC TTTGTTTTTT TTTCTTTTTT 1081 TGACACGATA AGAACATTTT TCTGTATTTC TAATATTTTA AATGAGATCG TAATAAGTTT 1141 ATTTAGCTCC ATCACATTAT TTTCGGAATA TTCATCGCTT GAAAACGGCG TAAAATAGGT 1201 TCCAAGTAAA TCATTGCAAA CAAATCTCCG TTTATCGTTC TAACAAAGTT TTTCGTTCAC 1261 TTTCTTATGT AAATCGTTTT CCCCACTTCT TCTTCACGGG AATGATCAAT TGAAAGGCCA 1321 TAAATTGGTT GTTTTCTCCA CATTTCGCAA CCAACATGCA GCTGTCGGAT AGGCAATAAA 1381 ATAATGTAAT CAATGAGTTT TCCGATTTAT CCTAACAGTT CTTTTTCTAG TGTTCCTATT 1441 CGAAAAAAGT ACTGTTATCC TTCTTTTTGG ACCGCCCATC TCAGGTGTCA AGACAATCGC 1501 AACACACTCA GAAAATGGGC GGAGTATGCC ACCCGGCCTT CATTCGTCGC TCCTTGTCTT 1561 CTCCATTTTC GTTTTTTTCC TGTTCTCCGT TCTTTTTTTG TACTTTTGTG ATAATGCTGA 1621 AGTGTTCTTT TTTTTCGCTT AACCGCATCT CTTGATTTAA TTCATATTTT CTTTCAGATT 1681 CATaTACAAT AACACCAAGA GCTGCTCTAA CAACTAATAA TAACAGAATC TAGAGGATCC 1741 CCGGGTACCG AGCTCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG TACCGGTGGG 1801 TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT TCAACGCGCT 1861 GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC 1921 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC 1981 CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCTTTGCCTG 2041 GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC CTGAGGCCGA 2101 TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA TCTACACCAA 2161 CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC CGACGGGTTG 2221 TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA CGCGAATTAT 2281 TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG TCGGTTACGG 2341 CCAGGACAGT CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG CCGGAGAAAA 2401 CCGCCTCGCG GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG ATCAGGATAT 2461 GTGGCGGATG AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA CTACACAAAT 2521 CAGCGATTTC CATGTTGCCA CTCGCTTTAA TGATGATTTC AGCCGCGCTG TACTGGAGGC 2581 TGAAGTTCAG ATGTGCGGCG AGTTGCGTGA CTACCTACGG GTAACAGTTT CTTTATGGCA 2641 GGGTGAAACG CAGGTCGCCA GCGGCACCGC GCCTTTCGGC GGTGAAATTA TCGATGAGCG 2701 TGGTGGTTAT GCCGATCGCG TCACACTACG TCTGAACGTC GAAAACCCGA AACTGTGGAG 2761 CGCCGAAATC CCGAATCTCT ATCGTGCGGT GGTTGAACTG CACACCGCCG ACGGCACGCT 2821 GATTGAAGCA GAAGCCTGCG ATGTCGGTTT CCGCGAGGTG CGGATTGAAA ATGGTCTGCT 2881 GCTGCTGAAC GGCAAGCCGT TGCTGATTCG AGGCGTTAAC CGTCACGAGC ATCATCCTCT 2941 GCATGGTCAG GTCATGGATG AGCAGACGAT GGTGCAGGAT ATCCTGCTGA TGAAGCAGAA 3001 CAACTTTAAC GCCGTGCGCT GTTCGCATTA TCCGAACCAT CCGCTGTGGT ACACGCTGTG 3061 CGACCGCTAC GGCCTGTATG TGGTGGATGA AGCCAATATT GAAACCCACG GCATGGTGCC 3121 AATGAATCGT CTGACCGATG ATCCGCGCTG GCTACCGGCG ATGAGCGAAC GCGTAACGCG 3181 AATGGTGCAG CGCGATCGTA ATCACCCGAG TGTGATCATC TGGTCGCTGG GGAATGAATC 3241 AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG 3301 CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT 3361 GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA 3421 ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATCCTT TGCGAATACG CCCACGCGAT 3481 GGGTAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT 3541 ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG ATTAAATATG ATGAAAACGG 3601 CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG CCGAACGATC GCCAGTTCTG 3661 TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA GCGCTGACGG AAGCAAAACA 3721 CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA 3781 CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC 3841 GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT 3901 GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA 3961 ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCCTGGCAGC AGTGGCGTCT 4021 GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC 4081 CAGCGAAATG GATTTTTGCA TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC 4141 AGGCTTTCTT TCACAGATGT GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA 4201 TCAGTTCACC CGTGCACCGC TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA 4261 CCCTAACGCC TGGGTCGAAC GCTGGAAGGC GGCGGGCCAT TACCAGGCCG AAGCAGCGTT 4321 GTTGCAGTGC ACGGCAGATA CACTTGCTGA TGCGGTGCTG ATTACGACCG CTCACGCGTG 4381 GCAGCATCAG GGGAAAACCT TATTTATCAG CCGGAAAACC TACCGGATTG ATGGTAGTGG 4441 TCAAATGGCG ATTACCGTTG ATGTTGAAGT GGCGAGCGAT ACACCGCATC CGGCGCGGAT 4501 TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC AGAGCGGGTA AACTGGCTCG GATTAGGGCC 4561 GCAAGAAAAC TATCCCGACC GCCTTACTGC CGCCTGTTTT GACCGCTGGG ATCTGCCATT 4621 GTCAGACATG TATACCCCGT ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG 4681 CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA 4741 CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC 4801 ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC 4861 AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA 4921 AAAATAATAG GCCTActagc gtcgacggta cCATGCATGg tattgatatc tgagctccgc 4981 atcggccgct gtcatcagat cgcCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT Tcctactagt cggccgtacg GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1455 LOCUS ADDGENE_1455 3633 bp DNA circular SYN 01/03/2005 DEFINITION pPD79_44 complete plasmid sequence KEYWORDS . SOURCE pPD79_44 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1455 at www.addgene.org FEATURES Location/Qualifiers source 1..3633 /organism="Addgene plasmid pPD79_44" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" CDS 31..1278 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf0" /translation="MTMITPSAQLTLTKGNKSWVPVEKMSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHM KRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVL LPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPGDPLVLERPPPR WSSNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ QLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQA LNRGLPLGFRFSALRHLDPKKLD" promoter 56..75 /label="T3_promoter" gene 103..810 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 286..309 /label="gfp.S65S" promoter complement(874..892) /label="T7_promoter" misc_feature 912..1060 /label="lacZ_a" misc_feature complement(903..919) /label="M13f20_primer" rep_origin 1078..1384 /label="f1_origin" promoter 1577..1605 /label="Amp_promoter" CDS 1647..2507 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 1647..2507 /label="Ampicillin" /gene="Ampicillin" rep_origin 2662..3281 /label="pBR322_origin" misc_feature 3497..3523 /label="lacI.C" promoter 3590..3619 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 121 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 181 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 241 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGCCAACAC TTGTCACTAC TTTCTCTTAT 301 GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CATATGAAAC gGCATGACTT TTTCAAGAGT 361 GCCATGCCCG AAGGTTATGT ACAGGAAAGA ACTATATTTT TCAAAGATGA CGGGAACTAC 421 AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG TTAATAGAAT CGAGTTAAAA 481 GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA AATTGGAATA CAACTATAAC 541 TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG GAATCAAAGT TAACTTCAAA 601 ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA ACAAAATACT 661 CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC ACAATCTGCC 721 CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT TGTAACAGCT 781 GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATGCC CGGGGGATCC ACTAGTTCTA 841 GAGCGGCCGC CACCGCGGTG GAGCTCCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC 901 TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT 961 CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT 1021 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA 1081 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 1141 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 1201 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 1261 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 1321 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 1381 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 1441 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA 1501 TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 1561 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 1621 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 1681 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 1741 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 1801 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 1861 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 1921 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 1981 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 2041 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 2101 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 2161 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 2221 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 2281 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 2341 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 2401 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 2461 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 2521 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 2581 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 2641 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 2701 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 2761 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 2821 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 2881 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 2941 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 3001 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 3061 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 3121 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 3181 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 3241 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 3301 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 3361 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 3421 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 3481 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 3541 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 3601 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGA // 1456 LOCUS ADDGENE_1456 8984 bp DNA circular SYN 01/03/2005 DEFINITION pPD80_08 complete plasmid sequence KEYWORDS . SOURCE pPD80_08 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1456 at www.addgene.org FEATURES Location/Qualifiers source 1..8984 /organism="Addgene plasmid pPD80_08" /mol_type="other DNA" misc_feature 74..98 /label="myo2.-1090" CDS 1049..5227 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf1" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMTAPKKK RKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG KLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTR AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQ LNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEA DTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQT RIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYL EDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLR VTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVV ELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQT MVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDD PRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYE GGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNS LGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCM NGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGK PLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQ WRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLL TPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVL ITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAE RVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQW RGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQL SAGRYHYQLVWCQK" misc_feature 1162..1185 /label="myo2.5'" misc_feature 1259..1300 /label="SynIVS.A" misc_feature 1334..1360 /label="SV40.NLS" gene 1376..2083 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 1559..1582 /label="gfp.S65S" gene 2180..5224 /label="lacZ" /gene="lacZ" promoter 6898..6926 /label="Amp_promoter" CDS 6968..7828 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6968..7828 /label="Ampicillin" /gene="Ampicillin" rep_origin 7983..8602 /label="pBR322_origin" misc_feature 8818..8844 /label="lacI.C" promoter 8911..8940 /label="lac_promoter" misc_feature 8975..8993 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAGCTT 61 GCATGCCTGC AGGTCGAGGC ATTTGAATTG GGGGTGGTGG ACAGTAACTG TCTGTAATAA 121 TAATTACTCC TGACCAGGTT GCAATTCGAG TTTTGATAAG CATAATTATA CCTTGTACAT 181 TGTGGGTTTT GTGCTGTGGA CGTTTTATTG TGACATCCCC ATAAGCTACA AGAACAAAAA 241 TGAAATgTAA AAGTATTGAA AACGTCGTAC ATTTTATATC TGAGTAGTAT CCTTTGCTTT 301 AAATGTCCAT AAAAcTAATT TTATAATCAA TAAAACAACG TTTGTAAATC AACTGAGTTT 361 ACAAGTAGAG ACATTGAGGG ATACTTTCAC TATGCTAAAG TGATAATCGA CCAAATAATA 421 ACTtCACTTT GGTATTTATT CCTGTCTTAT AAaTGTTATG TATGAATTAA ATTCATATGC 481 ATATGGCTCA CTCTGACAAt ttttAATAAT CTTCCAGATC AATATTGACT ACCGATGCGG 541 GTGGTCTTTT GCTTTGAATT CTGCTGAACT TTACACCCCG AACAGCAATG TGTGCTTCAG 601 CcTAAAAAAA AGTAAGTGTG TTAATCAGTg CCCCCGATTC TTCATTTTTT GCCCCTCTCT 661 CCCGTTTCGT CGGCAAAAGA AGAGAAAATA AAGATAAGTC TCAAGATAGG TTGGTAATCG 721 CTAAAGTGGT TGTGTGGATA AGAGTAGCAA AATGGCAGGA AGAGCACTTT GCGCGCACAC 781 ACTGTACTCA TTGTTCTGGA TAAAATTCTC TCGTTGTTTG CCGTCGGATG TCTGCCTCTC 841 TGCATTGAGC CGGCTTCTTC ACTATCTTTA GTTAACCTAA AATGCCGTTT CTTTTCTCGT 901 ATCCCcACTA TcCCGTTGAG GTTCTCTGCT CTCTTCGCTC CCTACCGCCA GCGAGCAACT 961 ATCCGTGGGG GCGCCTTGCT CGGAAGATGG GGGGGAAGAA AGAAGATTTT TGCTATTTGC 1021 ACTTGAGAAA GAGACTTTTC CTGCGTCGAT GGTTAGAGAA CAGTGTGCAG ACACTTTTCA 1081 GCTACCTAGA atTACAatTG GATATCCCCG CCTCCCAATC CACCCACCCA GGGAAAAAGA 1141 AGGGCTCGCC GAAAATCAAA GTTATCTCCA GGCTCGCGCA TCCCACCGAG CGGTTGACTT 1201 CTCTCCACCA CTTTTCATTT TAACCCTCGG GGTACGGGAT TGGCCAAAGG ACCCAAAGgt 1261 atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT GGAGGGTACC 1321 GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG GTaCCGGTAG AAAAAATGAG 1381 TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT GTTGAATTAG ATGGTGATGT 1441 TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT GATGCAACAT ACGGAAAACT 1501 TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT CCATGGCCAA CACTTGTCAC 1561 TACTTTCTCT TATGGTGTTC AATGCTTTTC AAGATACCCA GATCATATGA AACgGCATGA 1621 CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA AGAACTATAT TTTTCAAAGA 1681 TGACGGGAAC TACAAGACAC GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG 1741 AATCGAGTTA AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA 1801 ATACAACTAT AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA 1861 AGTTAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA 1921 TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC 1981 CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA 2041 GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA CTATACAAAT gcccggtGGG 2101 TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT TCAACGCGCT 2161 GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC 2221 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC 2281 CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCTTTGCCTG 2341 GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC CTGAGGCCGA 2401 TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA TCTACACCAA 2461 CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC CGACGGGTTG 2521 TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA CGCGAATTAT 2581 TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG TCGGTTACGG 2641 CCAGGACAGT CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG CCGGAGAAAA 2701 CCGCCTCGCG GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG ATCAGGATAT 2761 GTGGCGGATG AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA CTACACAAAT 2821 CAGCGATTTC CATGTTGCCA CTCGCTTTAA TGATGATTTC AGCCGCGCTG TACTGGAGGC 2881 TGAAGTTCAG ATGTGCGGCG AGTTGCGTGA CTACCTACGG GTAACAGTTT CTTTATGGCA 2941 GGGTGAAACG CAGGTCGCCA GCGGCACCGC GCCTTTCGGC GGTGAAATTA TCGATGAGCG 3001 TGGTGGTTAT GCCGATCGCG TCACACTACG TCTGAACGTC GAAAACCCGA AACTGTGGAG 3061 CGCCGAAATC CCGAATCTCT ATCGTGCGGT GGTTGAACTG CACACCGCCG ACGGCACGCT 3121 GATTGAAGCA GAAGCCTGCG ATGTCGGTTT CCGCGAGGTG CGGATTGAAA ATGGTCTGCT 3181 GCTGCTGAAC GGCAAGCCGT TGCTGATTCG AGGCGTTAAC CGTCACGAGC ATCATCCTCT 3241 GCATGGTCAG GTCATGGATG AGCAGACGAT GGTGCAGGAT ATCCTGCTGA TGAAGCAGAA 3301 CAACTTTAAC GCCGTGCGCT GTTCGCATTA TCCGAACCAT CCGCTGTGGT ACACGCTGTG 3361 CGACCGCTAC GGCCTGTATG TGGTGGATGA AGCCAATATT GAAACCCACG GCATGGTGCC 3421 AATGAATCGT CTGACCGATG ATCCGCGCTG GCTACCGGCG ATGAGCGAAC GCGTAACGCG 3481 AATGGTGCAG CGCGATCGTA ATCACCCGAG TGTGATCATC TGGTCGCTGG GGAATGAATC 3541 AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG 3601 CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT 3661 GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA 3721 ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATCCTT TGCGAATACG CCCACGCGAT 3781 GGGTAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT 3841 ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG ATTAAATATG ATGAAAACGG 3901 CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG CCGAACGATC GCCAGTTCTG 3961 TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA GCGCTGACGG AAGCAAAACA 4021 CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA 4081 CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC 4141 GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT 4201 GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA 4261 ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCCTGGCAGC AGTGGCGTCT 4321 GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC 4381 CAGCGAAATG GATTTTTGCA TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC 4441 AGGCTTTCTT TCACAGATGT GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA 4501 TCAGTTCACC CGTGCACCGC TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA 4561 CCCTAACGCC TGGGTCGAAC GCTGGAAGGC GGCGGGCCAT TACCAGGCCG AAGCAGCGTT 4621 GTTGCAGTGC ACGGCAGATA CACTTGCTGA TGCGGTGCTG ATTACGACCG CTCACGCGTG 4681 GCAGCATCAG GGGAAAACCT TATTTATCAG CCGGAAAACC TACCGGATTG ATGGTAGTGG 4741 TCAAATGGCG ATTACCGTTG ATGTTGAAGT GGCGAGCGAT ACACCGCATC CGGCGCGGAT 4801 TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC AGAGCGGGTA AACTGGCTCG GATTAGGGCC 4861 GCAAGAAAAC TATCCCGACC GCCTTACTGC CGCCTGTTTT GACCGCTGGG ATCTGCCATT 4921 GTCAGACATG TATACCCCGT ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG 4981 CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA 5041 CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC 5101 ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC 5161 AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA 5221 AAAATAATAG GGGCCGCTGT CATCAGATCG CCATCTCGCG CCCGTGCCTC TGACTTCTAA 5281 GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA CCCCCTATTT 5341 TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT TAAGTCACCT 5401 CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA AGTCGAAAAA 5461 AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC AATGTTCTGT 5521 GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA GAAAAAACCG 5581 CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT TTATTTCTTC 5641 GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT TTGGAGTATT 5701 TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT TTGCTTTTTG 5761 GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT AAAATCACAA 5821 GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA 5881 AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT TTGCCTTAAA 5941 TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTA AAATTAAACA TTTTTCTAAA 6001 TTTTATATGA TTTCTTTTAA ATTTGCAAAA ATTACTTAAA TTTGAATTCC CGCGCAAATG 6061 AGTGACTTCA TTTTCTGCAT TATTGTGTTT TCCGGCTATA TTAATAGGTA TTTGTTTGTG 6121 TTTTTCTTTA TTTTATGATT CGAACTCCAA TTTGTAAATT TTCGAACATA TTTCCCTAAA 6181 GAAAAAATAT GATTAATCTG GAAAAATTGG AAAATTATTT TTCAAATAAA AAACAAAGAA 6241 AAAAATGAAG AAAAACCTAT TAGTTTGGCC ATAAAACGCA AAAATGTCGA AAATGACGTC 6301 ACTCATCTGC GCGGGAAATC AAGAATAATT CGGCCTTTTT TATTTTTTTG GAAAATCGTA 6361 AAACATTTAG AAAAATTTTT TAATAGTTAT ATGGGACTTA TTCTGCATTT AGGGCAAAAG 6421 CCAGAGACTA CTCCACCGTT AACATGAATT ATGAATATTA TTGCGACAAG ACCCAAACAT 6481 TGATAAACCC AAATCTAGCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC 6541 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 6601 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT 6661 CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG 6721 TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT 6781 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 6841 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 6901 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 6961 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 7021 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 7081 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 7141 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 7201 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 7261 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 7321 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 7381 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 7441 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 7501 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 7561 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 7621 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 7681 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 7741 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 7801 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 7861 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 7921 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 7981 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 8041 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 8101 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 8161 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 8221 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 8281 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 8341 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG 8401 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 8461 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 8521 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 8581 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 8641 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA 8701 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 8761 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG 8821 CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT 8881 GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT 8941 GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT // 1457 LOCUS ADDGENE_1457 6554 bp DNA circular SYN 01/03/2005 DEFINITION pPD81_34 complete plasmid sequence KEYWORDS . SOURCE pPD81_34 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1457 at www.addgene.org FEATURES Location/Qualifiers source 1..6554 /organism="Addgene plasmid pPD81_34" /mol_type="other DNA" CDS 26..3316 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAA HPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVP SNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDG VNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMW RMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLW QGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTAD GTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDIL LMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPA MSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADT TATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAK YWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFA DRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAEN LSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQ FTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHA WQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLG LGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 36..56 /label="rf81.34" misc_feature 77..118 /label="SynIVS.A" misc_feature 152..178 /label="SV40.NLS" gene 269..3313 /label="lacZ" /gene="lacZ" promoter 4468..4496 /label="Amp_promoter" gene 4538..5398 /label="Ampicillin" /gene="Ampicillin" CDS 4538..5398 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5553..6172 /label="pBR322_origin" misc_feature 6388..6414 /label="lacI.C" promoter 6481..6510 /label="lac_promoter" misc_feature 6545..6563 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTAGAGGATC CCCGGGATTG 61 GCCAAAGGAC CCAAAGgtat gtttcgaatg atactaacat aacatagaac attttcagGA 121 GGACCCTTGG AGGGTACCGA GCTCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT 181 ACCGGTGGGT GAAGACCAGA AACAGCACCT CGAACTGAGC CGCGATATTG CCCAGCGTTT 241 CAACGCGCTG TATGGCGAGA TCGATCCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC 301 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG 361 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG 421 CTTTGCCTGG TTTCCGGCAC CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC 481 TGAGGCCGAT ACTGTCGTCG TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT 541 CTACACCAAC GTGACCTATC CCATTACGGT CAATCCGCCG TTTGTTCCCA CGGAGAATCC 601 GACGGGTTGT TACTCGCTCA CATTTAATGT TGATGAAAGC TGGCTACAGG AAGGCCAGAC 661 GCGAATTATT TTTGATGGCG TTAACTCGGC GTTTCATCTG TGGTGCAACG GGCGCTGGGT 721 CGGTTACGGC CAGGACAGTC GTTTGCCGTC TGAATTTGAC CTGAGCGCAT TTTTACGCGC 781 CGGAGAAAAC CGCCTCGCGG TGATGGTGCT GCGCTGGAGT GACGGCAGTT ATCTGGAAGA 841 TCAGGATATG TGGCGGATGA GCGGCATTTT CCGTGACGTC TCGTTGCTGC ATAAACCGAC 901 TACACAAATC AGCGATTTCC ATGTTGCCAC TCGCTTTAAT GATGATTTCA GCCGCGCTGT 961 ACTGGAGGCT GAAGTTCAGA TGTGCGGCGA GTTGCGTGAC TACCTACGGG TAACAGTTTC 1021 TTTATGGCAG GGTGAAACGC AGGTCGCCAG CGGCACCGCG CCTTTCGGCG GTGAAATTAT 1081 CGATGAGCGT GGTGGTTATG CCGATCGCGT CACACTACGT CTGAACGTCG AAAACCCGAA 1141 ACTGTGGAGC GCCGAAATCC CGAATCTCTA TCGTGCGGTG GTTGAACTGC ACACCGCCGA 1201 CGGCACGCTG ATTGAAGCAG AAGCCTGCGA TGTCGGTTTC CGCGAGGTGC GGATTGAAAA 1261 TGGTCTGCTG CTGCTGAACG GCAAGCCGTT GCTGATTCGA GGCGTTAACC GTCACGAGCA 1321 TCATCCTCTG CATGGTCAGG TCATGGATGA GCAGACGATG GTGCAGGATA TCCTGCTGAT 1381 GAAGCAGAAC AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA 1441 CACGCTGTGC GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG 1501 CATGGTGCCA ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG 1561 CGTAACGCGA ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG 1621 GAATGAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT CGCTGGATCA AATCTGTCGA 1681 TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC ACCACGGCCA CCGATATTAT 1741 TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC CCGGCTGTGC CGAAATGGTC 1801 CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG CTGATCCTTT GCGAATACGC 1861 CCACGCGATG GGTAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA 1921 TCCCCGTTTA CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA 1981 TGAAAACGGC AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG 2041 CCAGTTCTGT ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG CGCTGACGGA 2101 AGCAAAACAC CAGCAGCAGT TTTTCCAGTT CCGTTTATCC GGGCAAACCA TCGAAGTGAC 2161 CAGCGAATAC CTGTTCCGTC ATAGCGATAA CGAGCTCCTG CACTGGATGG TGGCGCTGGA 2221 TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC TCTGGATGTC GCTCCACAAG GTAAACAGTT 2281 GATTGAACTG CCTGAACTAC CGCAGCCGGA GAGCGCCGGG CAACTCTGGC TCACAGTACG 2341 CGTAGTGCAA CCGAACGCGA CCGCATGGTC AGAAGCCGGG CACATCAGCG CCTGGCAGCA 2401 GTGGCGTCTG GCGGAAAACC TCAGTGTGAC GCTCCCCGCC GCGTCCCACG CCATCCCGCA 2461 TCTGACCACC AGCGAAATGG ATTTTTGCAT CGAGCTGGGT AATAAGCGTT GGCAATTTAA 2521 CCGCCAGTCA GGCTTTCTTT CACAGATGTG GATTGGCGAT AAAAAACAAC TGCTGACGCC 2581 GCTGCGCGAT CAGTTCACCC GTGCACCGCT GGATAACGAC ATTGGCGTAA GTGAAGCGAC 2641 CCGCATTGAC CCTAACGCCT GGGTCGAACG CTGGAAGGCG GCGGGCCATT ACCAGGCCGA 2701 AGCAGCGTTG TTGCAGTGCA CGGCAGATAC ACTTGCTGAT GCGGTGCTGA TTACGACCGC 2761 TCACGCGTGG CAGCATCAGG GGAAAACCTT ATTTATCAGC CGGAAAACCT ACCGGATTGA 2821 TGGTAGTGGT CAAATGGCGA TTACCGTTGA TGTTGAAGTG GCGAGCGATA CACCGCATCC 2881 GGCGCGGATT GGCCTGAACT GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA ACTGGCTCGG 2941 ATTAGGGCCG CAAGAAAACT ATCCCGACCG CCTTACTGCC GCCTGTTTTG ACCGCTGGGA 3001 TCTGCCATTG TCAGACATGT ATACCCCGTA CGTCTTCCCG AGCGAAAACG GTCTGCGCTG 3061 CGGGACGCGC GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC AGTTCAACAT 3121 CAGCCGCTAC AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC TGCACGCGGA 3181 AGAAGGCACA TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG ACGACTCCTG 3241 GAGCCCGTCA GTATCGGCGG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT 3301 CTGGTGTCAA AAATAATAGG GGCCGCTGTC ATCAGATCGC CATCTCGCGC CCGTGCCTCT 3361 GACTTCTAAG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC 3421 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT 3481 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA 3541 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA 3601 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG 3661 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT 3721 TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT 3781 TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT 3841 TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA 3901 AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG 3961 CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT 4021 TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG 4081 CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 4141 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 4201 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 4261 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 4321 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 4381 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4441 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 4501 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 4561 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 4621 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 4681 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 4741 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 4801 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 4861 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 4921 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 4981 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 5041 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 5101 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 5161 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 5221 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 5281 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 5341 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 5401 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 5461 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 5521 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 5581 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 5641 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 5701 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 5761 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 5821 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 5881 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 5941 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 6001 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 6061 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 6121 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 6181 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 6241 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 6301 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 6361 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 6421 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 6481 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 6541 CACAGGAAAC AGCT // 1458 LOCUS ADDGENE_1458 6779 bp DNA circular SYN 01/03/2005 DEFINITION pPD87_18 complete plasmid sequence KEYWORDS . SOURCE pPD87_18 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1458 at www.addgene.org FEATURES Location/Qualifiers source 1..6779 /organism="Addgene plasmid pPD87_18" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..3541 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 377..403 /label="SV40.NLS" gene 494..3538 /label="lacZ" /gene="lacZ" promoter 4693..4721 /label="Amp_promoter" CDS 4763..5623 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4763..5623 /label="Ampicillin" /gene="Ampicillin" rep_origin 5778..6397 /label="pBR322_origin" misc_feature 6613..6639 /label="lacI.C" promoter 6706..6735 /label="lac_promoter" misc_feature 6770..6788 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA 961 CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT 1021 CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG 1081 GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA 1141 TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT 1201 TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA 1261 AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG 1321 TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA 1381 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA 1441 AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT 1501 GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG 1561 TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG CTGATGAAGC AGAACAACTT 1621 TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG 1681 CTACGGCCTG TATGTGGTGG ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA 1741 TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT 1801 GCAGCGCGAT CGTAATCACC CGAGTGTGAT CATCTGGTCG CTGGGGAATG AATCAGGCCA 1861 CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT CCCGCCCGGT 1921 GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC CGATGTACGC 1981 GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA AAAAATGGCT 2041 TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA TACGCCCACG CGATGGGTAA 2101 CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT CAGTATCCCC GTTTACAGGG 2161 CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA TATGATGAAA ACGGCAACCC 2221 GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC GATCGCCAGT TCTGTATGAA 2281 CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCGCTG ACGGAAGCAA AACACCAGCA 2341 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 2401 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 2461 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 2521 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 2581 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCCTGG CAGCAGTGGC GTCTGGCGGA 2641 AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA 2701 AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT 2761 TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC GCGATCAGTT 2821 CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA 2881 CGCCTGGGTC GAACGCTGGA AGGCGGCGGG CCATTACCAG GCCGAAGCAG CGTTGTTGCA 2941 GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG ACCGCTCACG CGTGGCAGCA 3001 TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG ATTGATGGTA GTGGTCAAAT 3061 GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG CATCCGGCGC GGATTGGCCT 3121 GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG CTCGGATTAG GGCCGCAAGA 3181 AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC TGGGATCTGC CATTGTCAGA 3241 CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT 3301 GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA 3361 ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT 3421 GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC 3481 GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA 3541 ATAGGGGCCG CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA 3601 TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA 3661 TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA 3721 ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT 3781 GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC 3841 TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA 3901 AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT 3961 CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA 4021 ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT 4081 CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG 4141 ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA 4201 GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG 4261 AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT 4321 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 4381 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 4441 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 4501 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 4561 CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT 4621 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 4681 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 4741 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 4801 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA 4861 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 4921 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT 4981 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT 5041 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 5101 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC 5161 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 5221 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 5281 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC 5341 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 5401 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC 5461 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 5521 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 5581 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA 5641 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA 5701 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 5761 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 5821 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 5881 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 5941 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 6001 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 6061 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 6121 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 6181 TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 6241 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 6301 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 6361 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 6421 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 6481 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 6541 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 6601 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 6661 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 6721 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCT // 1459 LOCUS ADDGENE_1459 6932 bp DNA circular SYN 01/03/2005 DEFINITION pPD87_50 complete plasmid sequence KEYWORDS . SOURCE pPD87_50 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1459 at www.addgene.org FEATURES Location/Qualifiers source 1..6932 /organism="Addgene plasmid pPD87_50" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..3694 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAVRCSHYPNHPLWYTLC DRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGN ESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKW SIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT*II*IFRL TEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQ GKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAA SHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDN DIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTL FISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYP DRLTAACFDRWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" gene 494..3691 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1595..1645 /label="SynIVS.E" misc_feature 2368..2418 /label="SynIVS.H" misc_feature 3349..3399 /label="SynIVS.K" promoter 4846..4874 /label="Amp_promoter" gene 4916..5776 /label="Ampicillin" /gene="Ampicillin" CDS 4916..5776 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5931..6550 /label="pBR322_origin" misc_feature 6766..6792 /label="lacI.C" promoter 6859..6888 /label="lac_promoter" misc_feature 6923..6941 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA 961 CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT 1021 CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG 1081 GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA 1141 TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT 1201 TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA 1261 AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG 1321 TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA 1381 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA 1441 AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT 1501 GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG 1561 TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATgtaagt ttaaactatt cgttactaac 1621 taactttaaa catttaaatt ttcagATCCT GCTGATGAAG CAGAACAACT TTAACGCCGT 1681 GCGCTGTTCG CATTATCCGA ACCATCCGCT GTGGTACACG CTGTGCGACC GCTACGGCCT 1741 GTATGTGGTG GATGAAGCCA ATATTGAAAC CCACGGCATG GTGCCAATGA ATCGTCTGAC 1801 CGATGATCCG CGCTGGCTAC CGGCGATGAG CGAACGCGTA ACGCGAATGG TGCAGCGCGA 1861 TCGTAATCAC CCGAGTGTGA TCATCTGGTC GCTGGGGAAT GAATCAGGCC ACGGCGCTAA 1921 TCACGACGCG CTGTATCGCT GGATCAAATC TGTCGATCCT TCCCGCCCGG TGCAGTATGA 1981 AGGCGGCGGA GCCGACACCA CGGCCACCGA TATTATTTGC CCGATGTACG CGCGCGTGGA 2041 TGAAGACCAG CCCTTCCCGG CTGTGCCGAA ATGGTCCATC AAAAAATGGC TTTCGCTACC 2101 TGGAGAGACG CGCCCGCTGA TCCTTTGCGA ATACGCCCAC GCGATGGGTA ACAGTCTTGG 2161 CGGTTTCGCT AAATACTGGC AGGCGTTTCG TCAGTATCCC CGTTTACAGG GCGGCTTCGT 2221 CTGGGACTGG GTGGATCAGT CGCTGATTAA ATATGATGAA AACGGCAACC CGTGGTCGGC 2281 TTACGGCGGT GATTTTGGCG ATACGCCGAA CGATCGCCAG TTCTGTATGA ACGGTCTGGT 2341 CTTTGCCGAC CGCACGCCGC ATCCAGCgta agtttaaaca ataacctact aactaacgta 2401 gataatttaa attttcagGC TGACGGAAGC AAAACACCAG CAGCAGTTTT TCCAGTTCCG 2461 TTTATCCGGG CAAACCATCG AAGTGACCAG CGAATACCTG TTCCGTCATA GCGATAACGA 2521 GCTCCTGCAC TGGATGGTGG CGCTGGATGG TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT 2581 GGATGTCGCT CCACAAGGTA AACAGTTGAT TGAACTGCCT GAACTACCGC AGCCGGAGAG 2641 CGCCGGGCAA CTCTGGCTCA CAGTACGCGT AGTGCAACCG AACGCGACCG CATGGTCAGA 2701 AGCCGGGCAC ATCAGCGCCT GGCAGCAGTG GCGTCTGGCG GAAAACCTCA GTGTGACGCT 2761 CCCCGCCGCG TCCCACGCCA TCCCGCATCT GACCACCAGC GAAATGGATT TTTGCATCGA 2821 GCTGGGTAAT AAGCGTTGGC AATTTAACCG CCAGTCAGGC TTTCTTTCAC AGATGTGGAT 2881 TGGCGATAAA AAACAACTGC TGACGCCGCT GCGCGATCAG TTCACCCGTG CACCGCTGGA 2941 TAACGACATT GGCGTAAGTG AAGCGACCCG CATTGACCCT AACGCCTGGG TCGAACGCTG 3001 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 3061 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 3121 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 3181 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 3241 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 3301 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTAgt aagtttaaac 3361 ttgatagtac taactaacat gtttcattta aattttcagT ACCCCGTACG TCTTCCCGAG 3421 CGAAAACGGT CTGCGCTGCG GGACGCGCGA ATTGAATTAT GGCCCACACC AGTGGCGCGG 3481 CGACTTCCAG TTCAACATCA GCCGCTACAG TCAACAGCAA CTGATGGAAA CCAGCCATCG 3541 CCATCTGCTG CACGCGGAAG AAGGCACATG GCTGAATATC GACGGTTTCC ATATGGGGAT 3601 TGGTGGCGAC GACTCCTGGA GCCCGTCAGT ATCGGCGGAA TTCCAACTGA GCGCCGGTCG 3661 CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGATCGCCA 3721 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 3781 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 3841 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 3901 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 3961 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 4021 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 4081 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 4141 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 4201 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 4261 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 4321 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 4381 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 4441 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 4501 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 4561 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 4621 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 4681 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 4741 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 4801 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 4861 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 4921 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 4981 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 5041 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 5101 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 5161 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 5221 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 5281 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 5341 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 5401 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 5461 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 5521 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 5581 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 5641 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 5701 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 5761 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 5821 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 5881 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 5941 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 6001 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 6061 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 6121 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 6181 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 6241 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 6301 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 6361 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 6421 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 6481 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 6541 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 6601 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 6661 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 6721 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 6781 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 6841 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 6901 GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1460 LOCUS ADDGENE_1460 6826 bp DNA circular SYN 01/03/2005 DEFINITION pPD88_22 complete plasmid sequence KEYWORDS . SOURCE pPD88_22 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1460 at www.addgene.org FEATURES Location/Qualifiers source 1..6826 /organism="Addgene plasmid pPD88_22" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..3541 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 377..403 /label="SV40.NLS" gene 494..3538 /label="lacZ" /gene="lacZ" misc_feature 3562..3612 /label="SynIVS.L" promoter 4740..4768 /label="Amp_promoter" gene 4810..5670 /label="Ampicillin" /gene="Ampicillin" CDS 4810..5670 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5825..6444 /label="pBR322_origin" misc_feature 6660..6686 /label="lacI.C" promoter 6753..6782 /label="lac_promoter" misc_feature 6817..6835 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA 961 CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT 1021 CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG 1081 GATGAGCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA 1141 TTTCCATGTT GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT 1201 TCAGATGTGC GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA 1261 AACGCAGGTC GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG 1321 TTATGCCGAT CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA 1381 AATCCCGAAT CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA 1441 AGCAGAAGCC TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT 1501 GAACGGCAAG CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG 1561 TCAGGTCATG GATGAGCAGA CGATGGTGCA GGATATCCTG CTGATGAAGC AGAACAACTT 1621 TAACGCCGTG CGCTGTTCGC ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG 1681 CTACGGCCTG TATGTGGTGG ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA 1741 TCGTCTGACC GATGATCCGC GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT 1801 GCAGCGCGAT CGTAATCACC CGAGTGTGAT CATCTGGTCG CTGGGGAATG AATCAGGCCA 1861 CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT CCCGCCCGGT 1921 GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC CGATGTACGC 1981 GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA AAAAATGGCT 2041 TTCGCTACCT GGAGAGACGC GCCCGCTGAT CCTTTGCGAA TACGCCCACG CGATGGGTAA 2101 CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT CAGTATCCCC GTTTACAGGG 2161 CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA TATGATGAAA ACGGCAACCC 2221 GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC GATCGCCAGT TCTGTATGAA 2281 CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCGCTG ACGGAAGCAA AACACCAGCA 2341 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 2401 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 2461 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 2521 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 2581 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCCTGG CAGCAGTGGC GTCTGGCGGA 2641 AAACCTCAGT GTGACGCTCC CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA 2701 AATGGATTTT TGCATCGAGC TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT 2761 TCTTTCACAG ATGTGGATTG GCGATAAAAA ACAACTGCTG ACGCCGCTGC GCGATCAGTT 2821 CACCCGTGCA CCGCTGGATA ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA 2881 CGCCTGGGTC GAACGCTGGA AGGCGGCGGG CCATTACCAG GCCGAAGCAG CGTTGTTGCA 2941 GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG ACCGCTCACG CGTGGCAGCA 3001 TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG ATTGATGGTA GTGGTCAAAT 3061 GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG CATCCGGCGC GGATTGGCCT 3121 GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG CTCGGATTAG GGCCGCAAGA 3181 AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC TGGGATCTGC CATTGTCAGA 3241 CATGTATACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT 3301 GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA 3361 ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT 3421 GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC 3481 GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA 3541 ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat 3601 ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA 3661 TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT 3721 TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA 3781 GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC 3841 ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT 3901 TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA 3961 TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT 4021 GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA 4081 GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG 4141 TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC 4201 AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA 4261 ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA 4321 TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT 4381 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 4441 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT 4501 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC 4561 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC 4621 CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA 4681 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 4741 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 4801 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT 4861 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 4921 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 4981 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG 5041 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 5101 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA 5161 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 5221 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 5281 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 5341 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 5401 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA 5461 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 5521 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 5581 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 5641 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 5701 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT 5761 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA 5821 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA 5881 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT 5941 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG 6001 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA 6061 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA 6121 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG 6181 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA 6241 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 6301 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC 6361 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC 6421 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT 6481 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT 6541 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG 6601 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 6661 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 6721 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG 6781 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1461 LOCUS ADDGENE_1461 7131 bp DNA circular SYN 01/03/2005 DEFINITION pPD88_27 complete plasmid sequence KEYWORDS . SOURCE pPD88_27 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1461 at www.addgene.org FEATURES Location/Qualifiers source 1..7131 /organism="Addgene plasmid pPD88_27" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1039 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLN LT" gene 494..3843 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" CDS 1078..3846 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNG KPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAV RCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQ RDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHDALYRWIKSVDPSRPVQY EGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGN SLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFC MNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFN RQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN* RRS**FSERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 1645..1695 /label="SynIVS.E" misc_feature 1952..2002 /label="SynIVS.F" misc_feature 2469..2519 /label="SynIVS.H" misc_feature 3095..3145 /label="SynIVS.J" misc_feature 3501..3551 /label="SynIVS.K" misc_feature 3867..3917 /label="SynIVS.L" promoter 5045..5073 /label="Amp_promoter" CDS 5115..5975 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5115..5975 /label="Ampicillin" /gene="Ampicillin" rep_origin 6130..6749 /label="pBR322_origin" misc_feature 6965..6991 /label="lacI.C" promoter 7058..7087 /label="lac_promoter" misc_feature 7122..7140 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC 661 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT 721 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC 781 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC 841 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA 901 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt 961 aagtttaatt aagttgataC TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG 1021 CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG 1081 GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC 1141 ATTTTCCGTG ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT 1201 GCCACTCGCT TTAATGATGA TTTCAGCCGC GCTGTACTGG AGGCTGAAGT TCAGATGTGC 1261 GGCGAGTTGC GTGACTACCT ACGGGTAACA GTTTCTTTAT GGCAGGGTGA AACGCAGGTC 1321 GCCAGCGGCA CCGCGCCTTT CGGCGGTGAA ATTATCGATG AGCGTGGTGG TTATGCCGAT 1381 CGCGTCACAC TACGTCTGAA CGTCGAAAAC CCGAAACTGT GGAGCGCCGA AATCCCGAAT 1441 CTCTATCGTG CGGTGGTTGA ACTGCACACC GCCGACGGCA CGCTGATTGA AGCAGAAGCC 1501 TGCGATGTCG GTTTCCGCGA GGTGCGGATT GAAAATGGTC TGCTGCTGCT GAACGGCAAG 1561 CCGTTGCTGA TTCGAGGCGT TAACCGTCAC GAGCATCATC CTCTGCATGG TCAGGTCATG 1621 GATGAGCAGA CGATGGTGCA GGATgtaagt ttaaactatt cgttactaac taactttaaa 1681 catttaaatt ttcagATCCT GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG 1741 CATTATCCGA ACCATCCGCT GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG 1801 GATGAAGCCA ATATTGAAAC CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG 1861 CGCTGGCTAC CGGCGATGAG CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC 1921 CCGAGTGTGA TCATCTGGTC GCTGGGGAAT Ggtaagttta aacagttgaa tactaactaa 1981 cggagatctt tgaaattttc agAATCAGGC CACGGCGCTA ATCACGACGC GCTGTATCGC 2041 TGGATCAAAT CTGTCGATCC TTCCCGCCCG GTGCAGTATG AAGGCGGCGG AGCCGACACC 2101 ACGGCCACCG ATATTATTTG CCCGATGTAC GCGCGCGTGG ATGAAGACCA GCCCTTCCCG 2161 GCTGTGCCGA AATGGTCCAT CAAAAAATGG CTTTCGCTAC CTGGAGAGAC GCGCCCGCTG 2221 ATCCTTTGCG AATACGCCCA CGCGATGGGT AACAGTCTTG GCGGTTTCGC TAAATACTGG 2281 CAGGCGTTTC GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG 2341 TCGCTGATTA AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC 2401 GATACGCCGA ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG 2461 CATCCAGCgt aagtttaaac aataacctac taactaacgt agataattta aattttcagG 2521 CTGACGGAAG CAAAACACCA GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC 2581 GAAGTGACCA GCGAATACCT GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG 2641 GCGCTGGATG GTAAGCCGCT GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT 2701 AAACAGTTGA TTGAACTGCC TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC 2761 ACAGTACGCG TAGTGCAACC GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCC 2821 TGGCAGCAGT GGCGTCTGGC GGAAAACCTC AGTGTGACGC TCCCCGCCGC GTCCCACGCC 2881 ATCCCGCATC TGACCACCAG CGAAATGGAT TTTTGCATCG AGCTGGGTAA TAAGCGTTGG 2941 CAATTTAACC GCCAGTCAGG CTTTCTTTCA CAGATGTGGA TTGGCGATAA AAAACAACTG 3001 CTGACGCCGC TGCGCGATCA GTTCACCCGT GCACCGCTGG ATAACGACAT TGGCGTAAGT 3061 GAAGCGACCC GCATTGACCC TAACGCCTGG GTCGgtaagt ttaaacaaag ttgtactaac 3121 taacgaagat cttgataatt ttcagAACGC TGGAAGGCGG CGGGCCATTA CCAGGCCGAA 3181 GCAGCGTTGT TGCAGTGCAC GGCAGATACA CTTGCTGATG CGGTGCTGAT TACGACCGCT 3241 CACGCGTGGC AGCATCAGGG GAAAACCTTA TTTATCAGCC GGAAAACCTA CCGGATTGAT 3301 GGTAGTGGTC AAATGGCGAT TACCGTTGAT GTTGAAGTGG CGAGCGATAC ACCGCATCCG 3361 GCGCGGATTG GCCTGAACTG CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA CTGGCTCGGA 3421 TTAGGGCCGC AAGAAAACTA TCCCGACCGC CTTACTGCCG CCTGTTTTGA CCGCTGGGAT 3481 CTGCCATTGT CAGACATGTA gtaagtttaa acttgatagt actaactaac atgtttcatt 3541 taaattttca gTACCCCGTA CGTCTTCCCG AGCGAAAACG GTCTGCGCTG CGGGACGCGC 3601 GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC AGTTCAACAT CAGCCGCTAC 3661 AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC TGCACGCGGA AGAAGGCACA 3721 TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG ACGACTCCTG GAGCCCGTCA 3781 GTATCGGCGG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA 3841 AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta actaacgagt 3901 aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT 3961 CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA 4021 AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT 4081 GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC 4141 CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG 4201 TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT 4261 TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT 4321 CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA 4381 ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT 4441 GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC 4501 GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT 4561 TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT 4621 CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC 4681 GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC 4741 TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG 4801 CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA 4861 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA 4921 AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA 4981 CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA 5041 TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT 5101 GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG 5161 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG 5221 ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG 5281 AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG 5341 GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT 5401 CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA 5461 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC 5521 TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC 5581 ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC 5641 GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC 5701 TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG 5761 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG 5821 GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA 5881 TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG 5941 CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA 6001 TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT 6061 TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 6121 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 6181 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 6241 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 6301 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 6361 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 6421 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 6481 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT 6541 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 6601 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 6661 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 6721 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 6781 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 6841 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 6901 GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC 6961 ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA 7021 TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC 7081 GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1462 LOCUS ADDGENE_1462 7335 bp DNA circular SYN 01/03/2005 DEFINITION pPD88_75 complete plasmid sequence KEYWORDS . SOURCE pPD88_75 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1462 at www.addgene.org FEATURES Location/Qualifiers source 1..7335 /organism="Addgene plasmid pPD88_75" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1090 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 377..403 /label="SV40.NLS" gene 494..4047 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 628..678 /label="SynIVS.B" CDS 1129..4050 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1306..1356 /label="SynIVS.D" misc_feature 1747..1797 /label="SynIVS.E" misc_feature 2054..2104 /label="SynIVS.F" misc_feature 2335..2385 /label="SynIVS.G" misc_feature 2622..2672 /label="SynIVS.H" misc_feature 2988..3038 /label="SynIVS.I" misc_feature 3299..3349 /label="SynIVS.J" misc_feature 3705..3755 /label="SynIVS.K" misc_feature 4071..4121 /label="SynIVS.L" promoter 5249..5277 /label="Amp_promoter" gene 5319..6179 /label="Ampicillin" /gene="Ampicillin" CDS 5319..6179 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6334..6953 /label="pBR322_origin" misc_feature 7169..7195 /label="lacI.C" promoter 7262..7291 /label="lac_promoter" misc_feature 7326..7344 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 421 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 481 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 541 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 601 CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact aactaacttg 661 ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA 721 AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC 781 CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA CCTATCCCAT 841 TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT 901 TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTTAA 961 CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg taagtttaat 1021 taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT GCCGTCTGAA 1081 TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC 1141 TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT 1201 GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC 1261 TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag tttaaacagg 1321 atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT GCGTGACTAC 1381 CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG CACCGCGCCT 1441 TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC ACTACGTCTG 1501 AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG TGCGGTGGTT 1561 GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT CGGTTTCCGC 1621 GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT GATTCGAGGC 1681 GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA GACGATGGTG 1741 CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa ttttcagATC 1801 CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC GAACCATCCG 1861 CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC CAATATTGAA 1921 ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT ACCGGCGATG 1981 AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT GATCATCTGG 2041 TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc tttgaaattt 2101 tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 2161 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 2221 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 2281 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG CGAGgtaagt 2341 ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC tCACGCGATG 2401 GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA TCCCCGTTTA 2461 CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA TGAAAACGGC 2521 AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG CCAGTTCTGT 2581 ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta aacaataacc 2641 tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA CCAGCAGCAG 2701 TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT 2761 CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC 2821 GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA 2881 CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG 2941 ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta agtttaaaca 3001 agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA CCTCAGTGTG 3061 ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC 3121 ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG 3181 TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG 3241 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGgt 3301 aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA ACGCTGGAAG 3361 GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT 3421 GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC 3481 AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA 3541 GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA 3601 GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT 3661 GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt ttaaacttga 3721 tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT CCCGAGCGAA 3781 AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG GCGCGGCGAC 3841 TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG CCATCGCCAT 3901 CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT GGGGATTGGT 3961 GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC CGGTCGCTAC 4021 CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa 4081 actgagttct actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC 4141 TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA 4201 CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT 4261 TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA 4321 AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC 4381 AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA 4441 GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT 4501 TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT 4561 TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT 4621 TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT 4681 AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG 4741 GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT 4801 TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG 4861 GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 4921 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 4981 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 5041 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 5101 AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 5161 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 5221 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 5281 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 5341 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 5401 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 5461 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 5521 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 5581 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 5641 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 5701 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 5761 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 5821 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 5881 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 5941 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 6001 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 6061 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 6121 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 6181 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 6241 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 6301 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 6361 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 6421 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 6481 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 6541 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 6601 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 6661 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 6721 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6781 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6841 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6901 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6961 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 7021 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 7081 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 7141 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 7201 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 7261 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 7321 ACACAGGAAA CAGCT // 1463 LOCUS ADDGENE_1463 7112 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_03 complete plasmid sequence KEYWORDS . SOURCE pPD89_03 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1463 at www.addgene.org FEATURES Location/Qualifiers source 1..7112 /organism="Addgene plasmid pPD89_03" /mol_type="other DNA" CDS 1..867 /label="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFA WFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENP TGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*L IFRTVVCRLNLT" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3824 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 405..455 /label="SynIVS.B" CDS 906..3827 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1083..1133 /label="SynIVS.D" misc_feature 1524..1574 /label="SynIVS.E" misc_feature 1831..1881 /label="SynIVS.F" misc_feature 2112..2162 /label="SynIVS.G" misc_feature 2399..2449 /label="SynIVS.H" misc_feature 2765..2815 /label="SynIVS.I" misc_feature 3076..3126 /label="SynIVS.J" misc_feature 3482..3532 /label="SynIVS.K" misc_feature 3848..3898 /label="SynIVS.L" promoter 5026..5054 /label="Amp_promoter" gene 5096..5956 /label="Ampicillin" /gene="Ampicillin" CDS 5096..5956 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6111..6730 /label="pBR322_origin" misc_feature 6946..6972 /label="lacI.C" promoter 7039..7068 /label="lac_promoter" misc_feature 7103..7121 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GaAGgtaagt ttaaacagat 421 ccatactaac taacttgttc tgacataatt ttcagCtTGA ATGGCGAATG GCGCTTTGCC 481 TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC 541 GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC 601 AACGTGACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT 661 TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT 721 ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC 781 GGCCAGgtaa gtttaattaa gttgataCTA ACTAACAAGA TCTGATTAAT TTTCagGACA 841 GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA AACCGCCTCG 901 CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT ATGTGGCGGA 961 TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA ATCAGCGATT 1021 TCCATGTTGC CACTCGCTTT AATGATGATT TCtccCGCGC TGTACTGGAG GCTGAAGTcC 1081 AGgtaagttt aaacaggatc ttactaacta acatgctaac actgaatttt cagATGTGCG 1141 GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA ACGCAGGTCG 1201 CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT TATGCCGATC 1261 GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA ATCCCGAATC 1321 TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA GCAGAAGCCT 1381 GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG AACGGCAAGC 1441 CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT CAGGTCATGG 1501 ATGAGCAGAC GATGGTGCAG GATgtaagtt taaactattc gttactaact aactttaaac 1561 atttaaattt tcagATCCTG CTGATGAAGC AGAACAACTT TAACGCCGTG CGCTGTTCGC 1621 ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG CTACGGCCTG TATGTGGTGG 1681 ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA TCGTCTGACC GATGATCCGC 1741 GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT GCAGCGCGAT CGTAATCACC 1801 CGAGTGTGAT CATCTGGTCG CTGGGGAATG gtaagtttaa acagttgaat actaactaac 1861 ggagatcttt gaaattttca gAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT 1921 GGATCAAATC TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA 1981 CGGCCACCGA TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG 2041 CTGTGCCGAA ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA 2101 TtCTTTGCGA Ggtaagttta aacagaactc tactaactaa cacattagat cctaattttc 2161 agTACGCtCA CGCGATGGGc AACAGTCTTG GCGGTTTCGC TAAATACTGG CAGGCGTTTC 2221 GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG TCGCTGATTA 2281 AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC GATACGCCGA 2341 ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG CATCCAGCgt 2401 aagtttaaac aataacctac taactaacgt agataattta aattttcagG CTGACGGAAG 2461 CAAAACACCA GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC GAAGTGACCA 2521 GCGAATACCT GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG GCGCTGGATG 2581 GTAAGCCGCT GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT AAACAGTTGA 2641 TTGAACTGCC TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC ACAGTACGCG 2701 TAGTGCAACC GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCa TGGCAGCAGT 2761 GGAGgtaagt ttaaacaaga tcctactaac taactctaca ttgatgaatt ttcagACTGG 2821 CGGAAAACCT CAGTGTGACG CTCCCCGCCG CGTCCCACGC CATCCCGCAT CTGACCACCA 2881 GCGAAATGGA TTTTTGCATC GAGCTGGGTA ATAAGCGTTG GCAATTTAAC CGCCAGTCAG 2941 GCTTTCTTTC ACAGATGTGG ATTGGCGATA AAAAACAACT GCTGACGCCG CTGCGCGATC 3001 AGTTCACCCG TGCACCGCTG GATAACGACA TTGGCGTAAG TGAAGCGACC CGCATTGACC 3061 CTAACGCCTG GGTCGgtaag tttaaacaaa gttgtactaa ctaacgaaga tcttgataat 3121 tttcagAACG CTGGAAGGCG GCGGGCCATT ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA 3181 CGGCAGATAC ACTTGCTGAT GCGGTGCTGA TTACGACCGC TCACGCGTGG CAGCATCAGG 3241 GGAAAACCTT ATTTATCAGC CGGAAAACCT ACCGGATTGA TGGTAGTGGT CAAATGGCGA 3301 TTACCGTTGA TGTTGAAGTG GCGAGCGATA CACCGCATCC GGCGCGGATT GGCCTGAACT 3361 GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA ACTGGCTCGG ATTAGGGCCG CAAGAAAACT 3421 ATCCCGACCG CCTTACTGCC GCCTGTTTTG ACCGCTGGGA TCTGCCATTG TCAGACATGT 3481 Agtaagttta aacttgatag tactaactaa catgtttcat ttaaattttc agTACCCCGT 3541 ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG CGAATTGAAT TATGGCCCAC 3601 ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA CAGTCAACAG CAACTGATGG 3661 AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC ATGGCTGAAT ATCGACGGTT 3721 TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC AGTATCGGCG GAATTCCAAC 3781 TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT 3841 CATCAGAgta agtttaaact gagttctact aactaacgag taatatttaa attttcagCA 3901 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 3961 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 4021 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 4081 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 4141 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 4201 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 4261 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 4321 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 4381 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 4441 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 4501 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 4561 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 4621 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 4681 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 4741 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 4801 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 4861 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 4921 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 4981 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 5041 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 5101 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 5161 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 5221 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 5281 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 5341 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 5401 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 5461 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 5521 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 5581 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 5641 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 5701 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 5761 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 5821 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 5881 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 5941 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 6001 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 6061 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 6121 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 6181 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 6241 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 6301 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 6361 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 6421 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 6481 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 6541 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 6601 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 6661 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 6721 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 6781 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 6841 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 6901 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 6961 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 7021 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 7081 GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1464 LOCUS ADDGENE_1464 7111 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_09 complete plasmid sequence KEYWORDS . SOURCE pPD89_09 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1464 at www.addgene.org FEATURES Location/Qualifiers source 1..7111 /organism="Addgene plasmid pPD89_09" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..138 /label="rf22.04" CDS 153..866 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 153..179 /label="SV40.NLS" gene 270..3823 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 404..454 /label="SynIVS.B" CDS 905..3826 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1082..1132 /label="SynIVS.D" misc_feature 1523..1573 /label="SynIVS.E" misc_feature 1830..1880 /label="SynIVS.F" misc_feature 2111..2161 /label="SynIVS.G" misc_feature 2398..2448 /label="SynIVS.H" misc_feature 2764..2814 /label="SynIVS.I" misc_feature 3075..3125 /label="SynIVS.J" misc_feature 3481..3531 /label="SynIVS.K" misc_feature 3847..3897 /label="SynIVS.L" promoter 5025..5053 /label="Amp_promoter" gene 5095..5955 /label="Ampicillin" /gene="Ampicillin" CDS 5095..5955 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6110..6729 /label="pBR322_origin" misc_feature 6945..6971 /label="lacI.C" promoter 7038..7067 /label="lac_promoter" misc_feature 7102..7120 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GAGGGTACCG AGCTCAGAAA AAATGACTGC TCCAAAGAAG AAGCGTAAGG 181 TACCGGTGGG TGAAGACCAG AAACAGCACC TCGAACTGAG CCGCGATATT GCCCAGCGTT 241 TCAACGCGCT GTATGGCGAG ATCGATCCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC 301 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA 361 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG aAGgtaagtt taaacagatc 421 catactaact aacttgttct gacataattt tcagCtTGAA TGGCGAATGG CGCTTTGCCT 481 GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG 541 ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA 601 ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT 661 GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA 721 TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG GTCGGTTACG 781 GCCAGgtaag tttaattaag ttgataCTAA CTAACAAGAT CTGATTAATT TTCagGACAG 841 TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC 901 GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT 961 GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA TCAGCGATTT 1021 CCATGTTGCC ACTCGCTTTA ATGATGATTT CtccCGCGCT GTACTGGAGG CTGAAGTcCA 1081 Ggtaagttta aacaggatct tactaactaa catgctaaca ctgaattttc agATGTGCGG 1141 CGAGTTGCGT GACTACCTAC GGGTAACAGT TTCTTTATGG CAGGGTGAAA CGCAGGTCGC 1201 CAGCGGCACC GCGCCTTTCG GCGGTGAAAT TATCGATGAG CGTGGTGGTT ATGCCGATCG 1261 CGTCACACTA CGTCTGAACG TCGAAAACCC GAAACTGTGG AGCGCCGAAA TCCCGAATCT 1321 CTATCGTGCG GTGGTTGAAC TGCACACCGC CGACGGCACG CTGATTGAAG CAGAAGCCTG 1381 CGATGTCGGT TTCCGCGAGG TGCGGATTGA AAATGGTCTG CTGCTGCTGA ACGGCAAGCC 1441 GTTGCTGATT CGAGGCGTTA ACCGTCACGA GCATCATCCT CTGCATGGTC AGGTCATGGA 1501 TGAGCAGACG ATGGTGCAGG ATgtaagttt aaactattcg ttactaacta actttaaaca 1561 tttaaatttt cagATCCTGC TGATGAAGCA GAACAACTTT AACGCCGTGC GCTGTTCGCA 1621 TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC TACGGCCTGT ATGTGGTGGA 1681 TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT CGTCTGACCG ATGATCCGCG 1741 CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG CAGCGCGATC GTAATCACCC 1801 GAGTGTGATC ATCTGGTCGC TGGGGAATGg taagtttaaa cagttgaata ctaactaacg 1861 gagatctttg aaattttcag AATCAGGCCA CGGCGCTAAT CACGACGCGC TGTATCGCTG 1921 GATCAAATCT GTCGATCCTT CCCGCCCGGT GCAGTATGAA GGCGGCGGAG CCGACACCAC 1981 GGCCACCGAT ATTATTTGCC CGATGTACGC GCGCGTGGAT GAAGACCAGC CCTTCCCGGC 2041 TGTGCCGAAA TGGTCCATCA AAAAATGGCT TTCGCTACCT GGAGAGACGC GCCCGCTGAT 2101 tCTTTGCGAG gtaagtttaa acagaactct actaactaac acattagatc ctaattttca 2161 gTACGCtCAC GCGATGGGcA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG 2221 TCAGTATCCC CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA 2281 ATATGATGAA AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA 2341 CGATCGCCAG TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCgta 2401 agtttaaaca ataacctact aactaacgta gataatttaa attttcagGC TGACGGAAGC 2461 AAAACACCAG CAGCAGTTTT TCCAGTTCCG TTTATCCGGG CAAACCATCG AAGTGACCAG 2521 CGAATACCTG TTCCGTCATA GCGATAACGA GCTCCTGCAC TGGATGGTGG CGCTGGATGG 2581 TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT GGATGTCGCT CCACAAGGTA AACAGTTGAT 2641 TGAACTGCCT GAACTACCGC AGCCGGAGAG CGCCGGGCAA CTCTGGCTCA CAGTACGCGT 2701 AGTGCAACCG AACGCGACCG CATGGTCAGA AGCCGGGCAC ATCAGCGCaT GGCAGCAGTG 2761 GAGgtaagtt taaacaagat cctactaact aactctacat tgatgaattt tcagACTGGC 2821 GGAAAACCTC AGTGTGACGC TCCCCGCCGC GTCCCACGCC ATCCCGCATC TGACCACCAG 2881 CGAAATGGAT TTTTGCATCG AGCTGGGTAA TAAGCGTTGG CAATTTAACC GCCAGTCAGG 2941 CTTTCTTTCA CAGATGTGGA TTGGCGATAA AAAACAACTG CTGACGCCGC TGCGCGATCA 3001 GTTCACCCGT GCACCGCTGG ATAACGACAT TGGCGTAAGT GAAGCGACCC GCATTGACCC 3061 TAACGCCTGG GTCGgtaagt ttaaacaaag ttgtactaac taacgaagat cttgataatt 3121 ttcagAACGC TGGAAGGCGG CGGGCCATTA CCAGGCCGAA GCAGCGTTGT TGCAGTGCAC 3181 GGCAGATACA CTTGCTGATG CGGTGCTGAT TACGACCGCT CACGCGTGGC AGCATCAGGG 3241 GAAAACCTTA TTTATCAGCC GGAAAACCTA CCGGATTGAT GGTAGTGGTC AAATGGCGAT 3301 TACCGTTGAT GTTGAAGTGG CGAGCGATAC ACCGCATCCG GCGCGGATTG GCCTGAACTG 3361 CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA CTGGCTCGGA TTAGGGCCGC AAGAAAACTA 3421 TCCCGACCGC CTTACTGCCG CCTGTTTTGA CCGCTGGGAT CTGCCATTGT CAGACATGTA 3481 gtaagtttaa acttgatagt actaactaac atgtttcatt taaattttca gTACCCCGTA 3541 CGTCTTCCCG AGCGAAAACG GTCTGCGCTG CGGGACGCGC GAATTGAATT ATGGCCCACA 3601 CCAGTGGCGC GGCGACTTCC AGTTCAACAT CAGCCGCTAC AGTCAACAGC AACTGATGGA 3661 AACCAGCCAT CGCCATCTGC TGCACGCGGA AGAAGGCACA TGGCTGAATA TCGACGGTTT 3721 CCATATGGGG ATTGGTGGCG ACGACTCCTG GAGCCCGTCA GTATCGGCGG AATTCCAACT 3781 GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA AAATAATAGG GGCCGCTGTC 3841 ATCAGAgtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagCAT 3901 CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT 3961 TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT 4021 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA 4081 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT 4141 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT 4201 TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG 4261 TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT CAAATCATGC 4321 TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA AGTTTATGAA 4381 ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG ATTTCGAGGA 4441 CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA AGATCGGAAG 4501 AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG AAAGTGGAGT 4561 AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC CAAACATAAC 4621 TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG 4681 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 4741 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA 4801 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 4861 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT GATACGCCTA 4921 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 4981 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 5041 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 5101 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 5161 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 5221 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 5281 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 5341 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 5401 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 5461 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 5521 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 5581 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 5641 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 5701 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 5761 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 5821 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 5881 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 5941 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 6001 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 6061 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 6121 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 6181 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 6241 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 6301 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 6361 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 6421 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 6481 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC 6541 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 6601 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 6661 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 6721 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 6781 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 6841 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 6901 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 6961 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 7021 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 7081 AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1465 LOCUS ADDGENE_1465 7116 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_17 complete plasmid sequence KEYWORDS . SOURCE pPD89_17 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1465 at www.addgene.org FEATURES Location/Qualifiers source 1..7116 /organism="Addgene plasmid pPD89_17" /mol_type="other DNA" misc_feature 79..120 /label="SynIVS.A" misc_feature 122..139 /label="rf22.11" misc_feature 158..184 /label="SV40.NLS" CDS 158..871 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 275..3828 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 409..459 /label="SynIVS.B" CDS 910..3831 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1087..1137 /label="SynIVS.D" misc_feature 1528..1578 /label="SynIVS.E" misc_feature 1835..1885 /label="SynIVS.F" misc_feature 2116..2166 /label="SynIVS.G" misc_feature 2403..2453 /label="SynIVS.H" misc_feature 2769..2819 /label="SynIVS.I" misc_feature 3080..3130 /label="SynIVS.J" misc_feature 3486..3536 /label="SynIVS.K" misc_feature 3852..3902 /label="SynIVS.L" promoter 5030..5058 /label="Amp_promoter" gene 5100..5960 /label="Ampicillin" /gene="Ampicillin" CDS 5100..5960 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6115..6734 /label="pBR322_origin" misc_feature 6950..6976 /label="lacI.C" promoter 7043..7072 /label="lac_promoter" misc_feature 7107..7125 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 181 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 241 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 301 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 361 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGaAGgt aagtttaaac 421 agatccatac taactaactt gttctgacat aattttcagC tTGAATGGCG AATGGCGCTT 481 TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA 541 GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC GGTTACGATG CGCCCATCTA 601 CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT GTTCCCACGG AGAATCCGAC 661 GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG CTACAGGAAG GCCAGACGCG 721 AATTATTTTT GATGGCGTTA ACTCGGCGTT TCATCTGTGG TGCAACGGGC GCTGGGTCGG 781 TTACGGCCAG gtaagtttaa ttaagttgat aCTAACTAAC AAGATCTGAT TAATTTTCag 841 GACAGTCGTT TGCCGTCTGA ATTTGACCTG AGCGCATTTT TACGCGCCGG AGAAAACCGC 901 CTCGCGGTGA TGGTGCTGCG CTGGAGTGAC GGCAGTTATC TGGAAGATCA GGATATGTGG 961 CGGATGAGCG GCATTTTCCG TGACGTCTCG TTGCTGCATA AACCGACTAC ACAAATCAGC 1021 GATTTCCATG TTGCCACTCG CTTTAATGAT GATTTCtccC GCGCTGTACT GGAGGCTGAA 1081 GTcCAGgtaa gtttaaacag gatcttacta actaacatgc taacactgaa ttttcagATG 1141 TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT TATGGCAGGG TGAAACGCAG 1201 GTCGCCAGCG GCACCGCGCC TTTCGGCGGT GAAATTATCG ATGAGCGTGG TGGTTATGCC 1261 GATCGCGTCA CACTACGTCT GAACGTCGAA AACCCGAAAC TGTGGAGCGC CGAAATCCCG 1321 AATCTCTATC GTGCGGTGGT TGAACTGCAC ACCGCCGACG GCACGCTGAT TGAAGCAGAA 1381 GCCTGCGATG TCGGTTTCCG CGAGGTGCGG ATTGAAAATG GTCTGCTGCT GCTGAACGGC 1441 AAGCCGTTGC TGATTCGAGG CGTTAACCGT CACGAGCATC ATCCTCTGCA TGGTCAGGTC 1501 ATGGATGAGC AGACGATGGT GCAGGATgta agtttaaact attcgttact aactaacttt 1561 aaacatttaa attttcagAT CCTGCTGATG AAGCAGAACA ACTTTAACGC CGTGCGCTGT 1621 TCGCATTATC CGAACCATCC GCTGTGGTAC ACGCTGTGCG ACCGCTACGG CCTGTATGTG 1681 GTGGATGAAG CCAATATTGA AACCCACGGC ATGGTGCCAA TGAATCGTCT GACCGATGAT 1741 CCGCGCTGGC TACCGGCGAT GAGCGAACGC GTAACGCGAA TGGTGCAGCG CGATCGTAAT 1801 CACCCGAGTG TGATCATCTG GTCGCTGGGG AATGgtaagt ttaaacagtt gaatactaac 1861 taacggagat ctttgaaatt ttcagAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT 1921 CGCTGGATCA AATCTGTCGA TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC 1981 ACCACGGCCA CCGATATTAT TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC 2041 CCGGCTGTGC CGAAATGGTC CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG 2101 CTGATtCTTT GCGAGgtaag tttaaacaga actctactaa ctaacacatt agatcctaat 2161 tttcagTACG CtCACGCGAT GGGcAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG 2221 TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG 2281 ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG 2341 CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA 2401 GCgtaagttt aaacaataac ctactaacta acgtagataa tttaaatttt cagGCTGACG 2461 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2521 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2581 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2641 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2701 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCaTGGCAG 2761 CAGTGGAGgt aagtttaaac aagatcctac taactaactc tacattgatg aattttcagA 2821 CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC 2881 ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG 2941 TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC 3001 GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT 3061 GACCCTAACG CCTGGGTCGg taagtttaaa caaagttgta ctaactaacg aagatcttga 3121 taattttcag AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 3181 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 3241 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 3301 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 3361 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 3421 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 3481 ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagTACC 3541 CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT GAATTATGGC 3601 CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA ACAGCAACTG 3661 ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT GAATATCGAC 3721 GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC GGCGGAATTC 3781 CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG 3841 CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc 3901 agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG 3961 CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT 4021 TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA 4081 TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA 4141 ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT 4201 AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT 4261 TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT 4321 CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT 4381 ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC 4441 GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC 4501 GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT 4561 GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC 4621 ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 4681 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 4741 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 4801 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 4861 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 4921 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 4981 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5041 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 5101 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 5161 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 5221 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 5281 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5341 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 5401 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 5461 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 5521 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 5581 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 5641 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 5701 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 5761 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 5821 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 5881 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 5941 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 6001 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6061 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6121 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6181 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6241 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 6301 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 6361 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 6421 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 6481 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 6541 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 6601 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 6661 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 6721 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 6781 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 6841 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 6901 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 6961 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 7021 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 7081 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1466 LOCUS ADDGENE_1466 7110 bp DNA circular SYN 01/03/2005 DEFINITION pPD89_20 complete plasmid sequence KEYWORDS . SOURCE pPD89_20 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1466 at www.addgene.org FEATURES Location/Qualifiers source 1..7110 /organism="Addgene plasmid pPD89_20" /mol_type="other DNA" CDS 26..865 /label="Orf1" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAA HPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVP ESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFN VDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRL NLT" misc_feature 36..56 /label="rf81.34" misc_feature 77..118 /label="SynIVS.A" misc_feature 152..178 /label="SV40.NLS" gene 269..3822 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 403..453 /label="SynIVS.B" CDS 904..3825 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1081..1131 /label="SynIVS.D" misc_feature 1522..1572 /label="SynIVS.E" misc_feature 1829..1879 /label="SynIVS.F" misc_feature 2110..2160 /label="SynIVS.G" misc_feature 2397..2447 /label="SynIVS.H" misc_feature 2763..2813 /label="SynIVS.I" misc_feature 3074..3124 /label="SynIVS.J" misc_feature 3480..3530 /label="SynIVS.K" misc_feature 3846..3896 /label="SynIVS.L" promoter 5024..5052 /label="Amp_promoter" gene 5094..5954 /label="Ampicillin" /gene="Ampicillin" CDS 5094..5954 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6109..6728 /label="pBR322_origin" misc_feature 6944..6970 /label="lacI.C" promoter 7037..7066 /label="lac_promoter" misc_feature 7101..7119 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTAGAGGATC CCCGGGATTG 61 GCCAAAGGAC CCAAAGgtat gtttcgaatg atactaacat aacatagaac attttcagGA 121 GGACCCTTGG AGGGTACCGA GCTCAGAAAA AATGACTGCT CCAAAGAAGA AGCGTAAGGT 181 ACCGGTGGGT GAAGACCAGA AACAGCACCT CGAACTGAGC CGCGATATTG CCCAGCGTTT 241 CAACGCGCTG TATGGCGAGA TCGATCCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC 301 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG 361 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGa AGgtaagttt aaacagatcc 421 atactaacta acttgttctg acataatttt cagCtTGAAT GGCGAATGGC GCTTTGCCTG 481 GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC CTGAGGCCGA 541 TACTGTCGTC GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA TCTACACCAA 601 CGTGACCTAT CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC CGACGGGTTG 661 TTACTCGCTC ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA CGCGAATTAT 721 TTTTGATGGC GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG TCGGTTACGG 781 CCAGgtaagt ttaattaagt tgataCTAAC TAACAAGATC TGATTAATTT TCagGACAGT 841 CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG CCGGAGAAAA CCGCCTCGCG 901 GTGATGGTGC TGCGCTGGAG TGACGGCAGT TATCTGGAAG ATCAGGATAT GTGGCGGATG 961 AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA CTACACAAAT CAGCGATTTC 1021 CATGTTGCCA CTCGCTTTAA TGATGATTTC tccCGCGCTG TACTGGAGGC TGAAGTcCAG 1081 gtaagtttaa acaggatctt actaactaac atgctaacac tgaattttca gATGTGCGGC 1141 GAGTTGCGTG ACTACCTACG GGTAACAGTT TCTTTATGGC AGGGTGAAAC GCAGGTCGCC 1201 AGCGGCACCG CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA TGCCGATCGC 1261 GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT CCCGAATCTC 1321 TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC TGATTGAAGC AGAAGCCTGC 1381 GATGTCGGTT TCCGCGAGGT GCGGATTGAA AATGGTCTGC TGCTGCTGAA CGGCAAGCCG 1441 TTGCTGATTC GAGGCGTTAA CCGTCACGAG CATCATCCTC TGCATGGTCA GGTCATGGAT 1501 GAGCAGACGA TGGTGCAGGA Tgtaagttta aactattcgt tactaactaa ctttaaacat 1561 ttaaattttc agATCCTGCT GATGAAGCAG AACAACTTTA ACGCCGTGCG CTGTTCGCAT 1621 TATCCGAACC ATCCGCTGTG GTACACGCTG TGCGACCGCT ACGGCCTGTA TGTGGTGGAT 1681 GAAGCCAATA TTGAAACCCA CGGCATGGTG CCAATGAATC GTCTGACCGA TGATCCGCGC 1741 TGGCTACCGG CGATGAGCGA ACGCGTAACG CGAATGGTGC AGCGCGATCG TAATCACCCG 1801 AGTGTGATCA TCTGGTCGCT GGGGAATGgt aagtttaaac agttgaatac taactaacgg 1861 agatctttga aattttcagA ATCAGGCCAC GGCGCTAATC ACGACGCGCT GTATCGCTGG 1921 ATCAAATCTG TCGATCCTTC CCGCCCGGTG CAGTATGAAG GCGGCGGAGC CGACACCACG 1981 GCCACCGATA TTATTTGCCC GATGTACGCG CGCGTGGATG AAGACCAGCC CTTCCCGGCT 2041 GTGCCGAAAT GGTCCATCAA AAAATGGCTT TCGCTACCTG GAGAGACGCG CCCGCTGATt 2101 CTTTGCGAGg taagtttaaa cagaactcta ctaactaaca cattagatcc taattttcag 2161 TACGCtCACG CGATGGGcAA CAGTCTTGGC GGTTTCGCTA AATACTGGCA GGCGTTTCGT 2221 CAGTATCCCC GTTTACAGGG CGGCTTCGTC TGGGACTGGG TGGATCAGTC GCTGATTAAA 2281 TATGATGAAA ACGGCAACCC GTGGTCGGCT TACGGCGGTG ATTTTGGCGA TACGCCGAAC 2341 GATCGCCAGT TCTGTATGAA CGGTCTGGTC TTTGCCGACC GCACGCCGCA TCCAGCgtaa 2401 gtttaaacaa taacctacta actaacgtag ataatttaaa ttttcagGCT GACGGAAGCA 2461 AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA AGTGACCAGC 2521 GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC GCTGGATGGT 2581 AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA ACAGTTGATT 2641 GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC AGTACGCGTA 2701 GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCaTG GCAGCAGTGG 2761 AGgtaagttt aaacaagatc ctactaacta actctacatt gatgaatttt cagACTGGCG 2821 GAAAACCTCA GTGTGACGCT CCCCGCCGCG TCCCACGCCA TCCCGCATCT GACCACCAGC 2881 GAAATGGATT TTTGCATCGA GCTGGGTAAT AAGCGTTGGC AATTTAACCG CCAGTCAGGC 2941 TTTCTTTCAC AGATGTGGAT TGGCGATAAA AAACAACTGC TGACGCCGCT GCGCGATCAG 3001 TTCACCCGTG CACCGCTGGA TAACGACATT GGCGTAAGTG AAGCGACCCG CATTGACCCT 3061 AACGCCTGGG TCGgtaagtt taaacaaagt tgtactaact aacgaagatc ttgataattt 3121 tcagAACGCT GGAAGGCGGC GGGCCATTAC CAGGCCGAAG CAGCGTTGTT GCAGTGCACG 3181 GCAGATACAC TTGCTGATGC GGTGCTGATT ACGACCGCTC ACGCGTGGCA GCATCAGGGG 3241 AAAACCTTAT TTATCAGCCG GAAAACCTAC CGGATTGATG GTAGTGGTCA AATGGCGATT 3301 ACCGTTGATG TTGAAGTGGC GAGCGATACA CCGCATCCGG CGCGGATTGG CCTGAACTGC 3361 CAGCTGGCGC AGGTAGCAGA GCGGGTAAAC TGGCTCGGAT TAGGGCCGCA AGAAAACTAT 3421 CCCGACCGCC TTACTGCCGC CTGTTTTGAC CGCTGGGATC TGCCATTGTC AGACATGTAg 3481 taagtttaaa cttgatagta ctaactaaca tgtttcattt aaattttcag TACCCCGTAC 3541 GTCTTCCCGA GCGAAAACGG TCTGCGCTGC GGGACGCGCG AATTGAATTA TGGCCCACAC 3601 CAGTGGCGCG GCGACTTCCA GTTCAACATC AGCCGCTACA GTCAACAGCA ACTGATGGAA 3661 ACCAGCCATC GCCATCTGCT GCACGCGGAA GAAGGCACAT GGCTGAATAT CGACGGTTTC 3721 CATATGGGGA TTGGTGGCGA CGACTCCTGG AGCCCGTCAG TATCGGCGGA ATTCCAACTG 3781 AGCGCCGGTC GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG GCCGCTGTCA 3841 TCAGAgtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcagCATC 3901 TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT 3961 CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG 4021 TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT 4081 TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA 4141 TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT 4201 TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT 4261 TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT 4321 CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA 4381 TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA TTTCGAGGAC 4441 GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA GATCGGAAGA 4501 AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA AAGTGGAGTA 4561 GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC AAACATAACT 4621 GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 4681 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 4741 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 4801 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 4861 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG ATACGCCTAT 4921 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 4981 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 5041 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 5101 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 5161 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 5221 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 5281 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG 5341 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 5401 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 5461 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 5521 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 5581 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 5641 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 5701 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 5761 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 5821 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 5881 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 5941 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 6001 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 6061 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 6121 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 6181 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 6241 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC 6301 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 6361 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 6421 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 6481 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG 6541 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 6601 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 6661 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 6721 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 6781 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 6841 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 6901 CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA 6961 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC 7021 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA 7081 GCGGATAACA ATTTCACACA GGAAACAGCT // 1467 LOCUS ADDGENE_1467 6096 bp DNA circular SYN 01/03/2005 DEFINITION pPD8_02 complete plasmid sequence KEYWORDS . SOURCE pPD8_02 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1467 at www.addgene.org FEATURES Location/Qualifiers source 1..6096 /organism="Addgene plasmid pPD8_02" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 3644..3672 /label="Amp_promoter" misc_feature complement(3971..4000) /label="SupTrp.3'" misc_feature complement(4015..4044) /label="SupTrp.5'" CDS 4080..4940 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4080..4940 /label="Ampicillin" /gene="Ampicillin" rep_origin 5095..5714 /label="pBR322_origin" misc_feature 5930..5956 /label="lacI.C" promoter 6023..6052 /label="lac_promoter" misc_feature 6087..6105 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGCCTACT AGTCGGCCGT ACGGGCCCTT AAGGCCGCCT GATGCGGTAT TTTCTCCTTA 3301 CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG 3361 CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT 3421 GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC 3481 AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT 3541 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 3601 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 3661 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATACAA TTTTCAGAAT ACGTTTTTTG 3721 TGGGCTTGGG TATATTGTTT TTAATGTTAT ACTTGCAGTC GTGAAATTTG ATTTTCAAAT 3781 TTGTAGAAAA ATCAAGAAAA TAATTGCAAC ATTCGCTTGT GTCAAAAACC AATTTCAACA 3841 AATTTTCGTG TGAGAAATAC ATTACCAGAA GGCATTTTTT CACACGATTA GCATTTTGGA 3901 CTACTTTATT AAATTTTTGC GTGTAATTTT GAATTAAATT GTATTATATT ACTACTTAAA 3961 AAACAAAAAA TTTGACCACT GAGCGGATCG AACGCCCAAC CTTTCGATCT AGAGTCGAAC 4021 GCGCTACCAT TGCGCCAAGC AGTCATGTTA TTCTCTCTTG TCATTGAAAA AGGAAGAGTA 4081 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 4141 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 4201 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 4261 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 4321 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 4381 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 4441 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 4501 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 4561 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 4621 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 4681 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 4741 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 4801 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 4861 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 4921 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 4981 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 5041 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 5101 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 5161 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 5221 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 5281 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 5341 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 5401 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 5461 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 5521 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 5581 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 5641 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 5701 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 5761 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 5821 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 5881 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 5941 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 6001 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 6061 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCT // 1468 LOCUS ADDGENE_1468 7369 bp DNA circular SYN 01/03/2005 DEFINITION pPD8_33 complete plasmid sequence KEYWORDS . SOURCE pPD8_33 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1468 at www.addgene.org FEATURES Location/Qualifiers source 1..7369 /organism="Addgene plasmid pPD8_33" /mol_type="other DNA" CDS 1..3240 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRVPSSEKMTAPKKKRKVPVGEDQKQH LELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR PSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTT QISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEI IDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVR IENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPN HPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPS VIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQ PFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPE LPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTT SEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATR IDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRH LLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK" misc_feature 76..102 /label="SV40.NLS" gene 193..3237 /label="lacZ" /gene="lacZ" promoter 4917..4945 /label="Amp_promoter" misc_feature complement(5244..5273) /label="SupTrp.3'" misc_feature complement(5288..5317) /label="SupTrp.5'" gene 5353..6213 /label="Ampicillin" /gene="Ampicillin" CDS 5353..6213 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6368..6987 /label="pBR322_origin" misc_feature 7203..7229 /label="lacI.C" promoter 7296..7325 /label="lac_promoter" misc_feature 7360..7378 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA 61 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 121 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 181 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 241 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 301 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG 361 GCACCAGAAG CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC 421 GTCGTCCCCT CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC 481 TATCCCATTA CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG 541 CTCACATTTA ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT 601 GGCGTTAACT CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGGAC 661 AGTCGTTTGC CGTCTGAATT TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC 721 GCGGTGATGG TGCTGCGCTG GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG 781 ATGAGCGGCA TTTTCCGTGA CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT 841 TTCCATGTTG CCACTCGCTT TAATGATGAT TTCAGCCGCG CTGTACTGGA GGCTGAAGTT 901 CAGATGTGCG GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA 961 ACGCAGGTCG CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT 1021 TATGCCGATC GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA 1081 ATCCCGAATC TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA 1141 GCAGAAGCCT GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG 1201 AACGGCAAGC CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT 1261 CAGGTCATGG ATGAGCAGAC GATGGTGCAG GATATCCTGC TGATGAAGCA GAACAACTTT 1321 AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT GTGCGACCGC 1381 TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT GCCAATGAAT 1441 CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC GCGAATGGTG 1501 CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGA ATCAGGCCAC 1561 GGCGCTAATC ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG 1621 CAGTATGAAG GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG 1681 CGCGTGGATG AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT 1741 TCGCTACCTG GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC 1801 AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC 1861 GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG 1921 TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC 1981 GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCGCTGA CGGAAGCAAA ACACCAGCAG 2041 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2101 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2161 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2221 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2281 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2341 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2401 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2461 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2521 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2581 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2641 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2701 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2761 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 2821 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 2881 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 2941 ATGTATACCC CGTACGTCTT CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG 3001 AATTATGGCC CACACCAGTG GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA 3061 CAGCAACTGA TGGAAACCAG CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG 3121 AATATCGACG GTTTCCATAT GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG 3181 GCGGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 3241 TAGGGGCCGC TGTCATCAGA TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT 3301 TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT 3361 TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA 3421 TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG 3481 CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT 3541 TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA 3601 AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC 3661 TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA 3721 TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC 3781 CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA 3841 TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG 3901 TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA 3961 ATACATTCCC AATATACCAA ACATAACTGT TTAAAATTAA ACATTTTTCT AAATTTTATA 4021 TGATTTCTTT TAAATTTGCA AAAATTACTT AAATTTGAAT TCCCGCGCAA ATGAGTGACT 4081 TCATTTTCTG CATTATTGTG TTTTCCGGCT ATATTAATAG GTATTTGTTT GTGTTTTTCT 4141 TTATTTTATG ATTCGAACTC CAATTTGTAA ATTTTCGAAC ATATTTCCCT AAAGAAAAAA 4201 TATGATTAAT CTGGAAAAAT TGGAAAATTA TTTTTCAAAT AAAAAACAAA GAAAAAAATG 4261 AAGAAAAACC TATTAGTTTG GCCATAAAAC GCAAAAATGT CGAAAATGAC GTCACTCATC 4321 TGCGCGGGAA ATCAAGAATA ATTCGGCCTT TTTTATTTTT TTGGAAAATC GTAAAACATT 4381 TAGAAAAATT TTTTAATAGT TATAGTGGGA CTGTATTCTG TCATTTAGGG CAAAAGCCAG 4441 AGACGCTACT CCACCGTTAA CATGAATTAT GAATATTATT GCGACAAGAC CCAAACATTG 4501 ATAAACCGCA AATCTAGCCT ACTAGTCGGC CGTACGGGCC CTTAAGGCCG CCTGATGCGG 4561 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATGGTGCAC TCTCAGTACA 4621 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG 4681 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG 4741 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC 4801 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT 4861 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA 4921 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA CAATTTTCAG 4981 AATACGTTTT TTGTGGGCTT GGGTATATTG TTTTTAATGT TATACTTGCA GTCGTGAAAT 5041 TTGATTTTCA AATTTGTAGA AAAATCAAGA AAATAATTGC AACATTCGCT TGTGTCAAAA 5101 ACCAATTTCA ACAAATTTTC GTGTGAGAAA TACATTACCA GAAGGCATTT TTTCACACGA 5161 TTAGCATTTT GGACTACTTT ATTAAATTTT TGCGTGTAAT TTTGAATTAA ATTGTATTAT 5221 ATTACTACTT AAAAAACAAA AAATTTGACC ACTGAGCGGA TCGAACGCCC AACCTTTCGA 5281 TCTAGAGTCG AACGCGCTAC CATTGCGCCA AGCAGTCATG TTATTCTCTC TTGTCATTGA 5341 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 5401 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 5461 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 5521 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 5581 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 5641 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5701 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5761 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5821 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5881 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5941 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 6001 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 6061 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 6121 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 6181 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 6241 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 6301 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 6361 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 6421 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 6481 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 6541 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 6601 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6661 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6721 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA 6781 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6841 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6901 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6961 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 7021 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 7081 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 7141 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 7201 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 7261 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 7321 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCT // 1469 LOCUS ADDGENE_1469 6603 bp DNA circular SYN 01/03/2005 DEFINITION pPD90_23 complete plasmid sequence KEYWORDS . SOURCE pPD90_23 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1469 at www.addgene.org FEATURES Location/Qualifiers source 1..6603 /organism="Addgene plasmid pPD90_23" /mol_type="other DNA" CDS 1..3318 /label="lacZ" /gene="lacZ" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRL TDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPV QYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAM GNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQ FCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVAL DGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISA WQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKK QLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQ VAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3315 /label="lacZ" /gene="lacZ" misc_feature 3339..3389 /label="SynIVS.L" promoter 4517..4545 /label="Amp_promoter" gene 4587..5447 /label="Ampicillin" /gene="Ampicillin" CDS 4587..5447 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5602..6221 /label="pBR322_origin" misc_feature 6437..6463 /label="lacI.C" promoter 6530..6559 /label="lac_promoter" misc_feature 6594..6612 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG 1381 ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG 1441 TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC 1501 GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 1561 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG 1621 GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC 1681 GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT 1741 ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG 1801 TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 1861 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG 1921 TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT 1981 GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT 2041 CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG 2101 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2161 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2221 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 2281 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 2341 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG 2401 CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 2461 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT 2521 AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG 2581 CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG 2641 ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC 2701 GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 2761 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT 2821 GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT 2881 CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC 2941 GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG 3001 GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 3061 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC 3121 ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG 3181 GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC 3241 TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA TTACCAACTT 3301 GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac tgagttctac 3361 taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT 3421 CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT 3481 GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT 3541 AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA 3601 TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT 3661 ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA 3721 CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC 3781 ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT 3841 TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG 3901 GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT 3961 ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG 4021 GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG 4081 ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC 4141 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 4201 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 4261 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 4321 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 4381 CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 4441 TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT 4501 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 4561 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 4621 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 4681 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 4741 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG 4801 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 4861 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 4921 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 4981 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 5041 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC 5101 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 5161 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 5221 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 5281 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 5341 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA 5401 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG 5461 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 5521 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 5581 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 5641 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 5701 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 5761 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 5821 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 5881 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 5941 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 6001 AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 6061 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 6121 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 6181 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 6241 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 6301 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA 6361 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 6421 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 6481 AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA 6541 TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA 6601 GCT // 1470 LOCUS ADDGENE_1470 6709 bp DNA circular SYN 01/03/2005 DEFINITION pPD90_28 complete plasmid sequence KEYWORDS . SOURCE pPD90_28 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1470 at www.addgene.org FEATURES Location/Qualifiers source 1..6709 /organism="Addgene plasmid pPD90_28" /mol_type="other DNA" CDS 1..3471 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDG SYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRD YLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYR AVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMD EQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYV VDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGAN HDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQ QFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIEL PELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHL TTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEA TRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTY RIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAAC FDRWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDF QFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGR YHYQLVWCQK" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3468 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1372..1422 /label="SynIVS.E" misc_feature 2145..2195 /label="SynIVS.H" misc_feature 3126..3176 /label="SynIVS.K" promoter 4623..4651 /label="Amp_promoter" gene 4693..5553 /label="Ampicillin" /gene="Ampicillin" CDS 4693..5553 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5708..6327 /label="pBR322_origin" misc_feature 6543..6569 /label="lacI.C" promoter 6636..6665 /label="lac_promoter" misc_feature 6700..6718 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC 781 GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA 841 GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG 901 ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 961 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT 1021 TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT 1081 ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG 1141 AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC 1201 GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 1261 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG 1321 CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA Tgtaagttta 1381 aactattcgt tactaactaa ctttaaacat ttaaattttc agATCCTGCT GATGAAGCAG 1441 AACAACTTTA ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG 1501 TGCGACCGCT ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG 1561 CCAATGAATC GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG 1621 CGAATGGTGC AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGAA 1681 TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT CGATCCTTCC 1741 CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT TATTTGCCCG 1801 ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG GTCCATCAAA 1861 AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATCC TTTGCGAATA CGCCCACGCG 1921 ATGGGTAACA GTCTTGGCGG TTTCGCTAAA TACTGGCAGG CGTTTCGTCA GTATCCCCGT 1981 TTACAGGGCG GCTTCGTCTG GGACTGGGTG GATCAGTCGC TGATTAAATA TGATGAAAAC 2041 GGCAACCCGT GGTCGGCTTA CGGCGGTGAT TTTGGCGATA CGCCGAACGA TCGCCAGTTC 2101 TGTATGAACG GTCTGGTCTT TGCCGACCGC ACGCCGCATC CAGCgtaagt ttaaacaata 2161 acctactaac taacgtagat aatttaaatt ttcagGCTGA CGGAAGCAAA ACACCAGCAG 2221 CAGTTTTTCC AGTTCCGTTT ATCCGGGCAA ACCATCGAAG TGACCAGCGA ATACCTGTTC 2281 CGTCATAGCG ATAACGAGCT CCTGCACTGG ATGGTGGCGC TGGATGGTAA GCCGCTGGCA 2341 AGCGGTGAAG TGCCTCTGGA TGTCGCTCCA CAAGGTAAAC AGTTGATTGA ACTGCCTGAA 2401 CTACCGCAGC CGGAGAGCGC CGGGCAACTC TGGCTCACAG TACGCGTAGT GCAACCGAAC 2461 GCGACCGCAT GGTCAGAAGC CGGGCACATC AGCGCCTGGC AGCAGTGGCG TCTGGCGGAA 2521 AACCTCAGTG TGACGCTCCC CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA 2581 ATGGATTTTT GCATCGAGCT GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT 2641 CTTTCACAGA TGTGGATTGG CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC 2701 ACCCGTGCAC CGCTGGATAA CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC 2761 GCCTGGGTCG AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 2821 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 2881 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 2941 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 3001 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 3061 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 3121 ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagTACC 3181 CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT GAATTATGGC 3241 CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA ACAGCAACTG 3301 ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT GAATATCGAC 3361 GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC GGCGGAATTC 3421 CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG 3481 CTGTCATCAG ATCGCCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA 3541 ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA 3601 ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT 3661 GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC 3721 CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT 3781 TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA 3841 TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT 3901 CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT 3961 CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT 4021 TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA 4081 TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG 4141 ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC 4201 CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC 4261 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 4321 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 4381 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 4441 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG 4501 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 4561 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4621 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4681 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4741 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4801 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4861 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4921 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4981 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 5041 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 5101 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 5161 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 5221 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 5281 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 5341 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 5401 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 5461 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 5521 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 5581 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 5641 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 5701 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 5761 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 5821 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 5881 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 5941 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 6001 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 6061 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA 6121 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 6181 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 6241 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 6301 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 6361 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 6421 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 6481 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 6541 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 6601 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 6661 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCT // 1471 LOCUS ADDGENE_1471 6908 bp DNA circular SYN 01/03/2005 DEFINITION pPD90_31 complete plasmid sequence KEYWORDS . SOURCE pPD90_31 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1471 at www.addgene.org FEATURES Location/Qualifiers source 1..6908 /organism="Addgene plasmid pPD90_31" /mol_type="other DNA" CDS 1..816 /label="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW RVPSSEKMTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPG VTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDL PEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQE GQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLNLT" misc_feature 79..120 /label="SynIVS.A" misc_feature 154..180 /label="SV40.NLS" gene 271..3620 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" CDS 855..3623 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNG KPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAV RCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQ RDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHDALYRWIKSVDPSRPVQY EGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGN SLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFC MNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFN RQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN* RRS**FSERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 1422..1472 /label="SynIVS.E" misc_feature 1729..1779 /label="SynIVS.F" misc_feature 2246..2296 /label="SynIVS.H" misc_feature 2872..2922 /label="SynIVS.J" misc_feature 3278..3328 /label="SynIVS.K" misc_feature 3644..3694 /label="SynIVS.L" promoter 4822..4850 /label="Amp_promoter" gene 4892..5752 /label="Ampicillin" /gene="Ampicillin" CDS 4892..5752 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 5907..6526 /label="pBR322_origin" misc_feature 6742..6768 /label="lacI.C" promoter 6835..6864 /label="lac_promoter" misc_feature 6899..6917 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA GAAGCGTAAG 181 GTACCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 241 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 301 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 361 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG 421 CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT 481 CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC 541 ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT 601 CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 661 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG 721 GTCGGTTACG GCCAGgtaag tttaattaag ttgataCTAA CTAACAAGAT CTGATTAATT 781 TTCagGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC GCCGGAGAAA 841 ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA 901 TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA 961 TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT GTACTGGAGG 1021 CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT TCTTTATGGC 1081 AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT ATCGATGAGC 1141 GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA 1201 GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC 1261 TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA AATGGTCTGC 1321 TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG CATCATCCTC 1381 TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA Tgtaagttta aactattcgt 1441 tactaactaa ctttaaacat ttaaattttc agATCCTGCT GATGAAGCAG AACAACTTTA 1501 ACGCCGTGCG CTGTTCGCAT TATCCGAACC ATCCGCTGTG GTACACGCTG TGCGACCGCT 1561 ACGGCCTGTA TGTGGTGGAT GAAGCCAATA TTGAAACCCA CGGCATGGTG CCAATGAATC 1621 GTCTGACCGA TGATCCGCGC TGGCTACCGG CGATGAGCGA ACGCGTAACG CGAATGGTGC 1681 AGCGCGATCG TAATCACCCG AGTGTGATCA TCTGGTCGCT GGGGAATGgt aagtttaaac 1741 agttgaatac taactaacgg agatctttga aattttcagA ATCAGGCCAC GGCGCTAATC 1801 ACGACGCGCT GTATCGCTGG ATCAAATCTG TCGATCCTTC CCGCCCGGTG CAGTATGAAG 1861 GCGGCGGAGC CGACACCACG GCCACCGATA TTATTTGCCC GATGTACGCG CGCGTGGATG 1921 AAGACCAGCC CTTCCCGGCT GTGCCGAAAT GGTCCATCAA AAAATGGCTT TCGCTACCTG 1981 GAGAGACGCG CCCGCTGATC CTTTGCGAAT ACGCCCACGC GATGGGTAAC AGTCTTGGCG 2041 GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG TTTACAGGGC GGCTTCGTCT 2101 GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA CGGCAACCCG TGGTCGGCTT 2161 ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT CTGTATGAAC GGTCTGGTCT 2221 TTGCCGACCG CACGCCGCAT CCAGCgtaag tttaaacaat aacctactaa ctaacgtaga 2281 taatttaaat tttcagGCTG ACGGAAGCAA AACACCAGCA GCAGTTTTTC CAGTTCCGTT 2341 TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT CCGTCATAGC GATAACGAGC 2401 TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC AAGCGGTGAA GTGCCTCTGG 2461 ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA ACTACCGCAG CCGGAGAGCG 2521 CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA CGCGACCGCA TGGTCAGAAG 2581 CCGGGCACAT CAGCGCCTGG CAGCAGTGGC GTCTGGCGGA AAACCTCAGT GTGACGCTCC 2641 CCGCCGCGTC CCACGCCATC CCGCATCTGA CCACCAGCGA AATGGATTTT TGCATCGAGC 2701 TGGGTAATAA GCGTTGGCAA TTTAACCGCC AGTCAGGCTT TCTTTCACAG ATGTGGATTG 2761 GCGATAAAAA ACAACTGCTG ACGCCGCTGC GCGATCAGTT CACCCGTGCA CCGCTGGATA 2821 ACGACATTGG CGTAAGTGAA GCGACCCGCA TTGACCCTAA CGCCTGGGTC Ggtaagttta 2881 aacaaagttg tactaactaa cgaagatctt gataattttc agAACGCTGG AAGGCGGCGG 2941 GCCATTACCA GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG 3001 TGCTGATTAC GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA 3061 AAACCTACCG GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA 3121 GCGATACACC GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC 3181 GGGTAAACTG GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT 3241 GTTTTGACCG CTGGGATCTG CCATTGTCAG ACATGTAgta agtttaaact tgatagtact 3301 aactaacatg tttcatttaa attttcagTA CCCCGTACGT CTTCCCGAGC GAAAACGGTC 3361 TGCGCTGCGG GACGCGCGAA TTGAATTATG GCCCACACCA GTGGCGCGGC GACTTCCAGT 3421 TCAACATCAG CCGCTACAGT CAACAGCAAC TGATGGAAAC CAGCCATCGC CATCTGCTGC 3481 ACGCGGAAGA AGGCACATGG CTGAATATCG ACGGTTTCCA TATGGGGATT GGTGGCGACG 3541 ACTCCTGGAG CCCGTCAGTA TCGGCGGAAT TCCAACTGAG CGCCGGTCGC TACCATTACC 3601 AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt taaactgagt 3661 tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC CTCTGACTTC 3721 TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA 3781 TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA 3841 CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA 3901 AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC 3961 TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA 4021 CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC 4081 TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT 4141 ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT 4201 TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA 4261 CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT 4321 GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT 4381 AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG TCGGCCGTAC 4441 GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 4501 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 4561 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT 4621 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC 4681 CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT 4741 AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT 4801 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA 4861 AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT 4921 TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA 4981 AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA 5041 CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT 5101 TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG 5161 TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA 5221 TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA 5281 CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT 5341 GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC 5401 CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA 5461 ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA 5521 GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC 5581 TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA 5641 TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA 5701 ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA 5761 CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT 5821 CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT 5881 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT 5941 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC 6001 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC 6061 AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC 6121 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC 6181 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG 6241 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA 6301 CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA 6361 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC 6421 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG 6481 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT 6541 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT 6601 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA 6661 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC 6721 CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG 6781 CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA 6841 CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG 6901 AAACAGCT // 1472 LOCUS ADDGENE_1472 4438 bp DNA circular SYN 01/03/2005 DEFINITION pPD91_14 complete plasmid sequence KEYWORDS . SOURCE pPD91_14 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1472 at www.addgene.org FEATURES Location/Qualifiers source 1..4438 /organism="Addgene plasmid pPD91_14" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1135 /label="GFP(variant)" /gene="GFP(variant)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAM PEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN SHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" gene 419..1126 /label="GFP(variant)" /gene="GFP(variant)" misc_feature 602..625 /label="gfp.S65S" promoter 2352..2380 /label="Amp_promoter" CDS 2422..3282 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2422..3282 /label="Ampicillin" /gene="Ampicillin" rep_origin 3437..4056 /label="pBR322_origin" misc_feature 4272..4298 /label="lacI.C" promoter 4365..4394 /label="lac_promoter" misc_feature 4429..4447 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGC CAACACTTGT 601 CACTACTTTC TCTTATGGTG TTCAATGCTT TTCAAGATAC CCAGATCATA TGAAACgGCA 661 TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA 721 AGATGACGGG AACTACAAGA CACGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA 781 TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAATT 841 GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA GACAAACAAA AGAATGGAAT 901 CAAAGTTAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA 961 TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT 1021 GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT 1081 TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC 1141 GTAGAATTCC AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA 1201 TAGGGGCCGC TGTCATCAGA TCGCCATCTC GCGCCCGTGC CTCTGACTTC TAAGTCCAAT 1261 TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC CCACCCCCTA TTTTTGTTAT 1321 TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC TTTTAAGTCA CCTCTAACAA 1381 TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA AAAAGTCGAA AAAAATTGTG 1441 CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA CACAATGTTC TGTGTACACT 1501 TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC ATAGAAAAAA CCGCACACAA 1561 AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA TTTTTATTTC TTCGCACGTC 1621 TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA 1681 TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG CTTTTGCTTT TTGGGGTTTC 1741 CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT GCTAAAATCA CAAGTATTGA 1801 TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG 1861 TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG TTTTTGCCTT AAATGACAGA 1921 ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG TCGGCCGTAC GGGCCCTTTC 1981 GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG 2041 TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG 2101 GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG 2161 TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC 2221 GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT 2281 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 2341 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA 2401 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT 2461 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT 2521 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG 2581 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG 2641 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA 2701 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT 2761 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC 2821 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG 2881 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 2941 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT 3001 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA 3061 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT 3121 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 3181 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 3241 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC 3301 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 3361 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 3421 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 3481 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 3541 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC 3601 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 3661 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 3721 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 3781 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 3841 GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 3901 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 3961 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 4021 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 4081 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 4141 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 4201 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG 4261 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC 4321 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT 4381 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCT // 1473 LOCUS ADDGENE_1473 4638 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_48 complete plasmid sequence KEYWORDS . SOURCE pPD93_48 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1473 at www.addgene.org FEATURES Location/Qualifiers source 1..4638 /organism="Addgene plasmid pPD93_48" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65C" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1374..1424 /label="SynIVS.L" promoter 2552..2580 /label="Amp_promoter" gene 2622..3482 /label="Ampicillin" /gene="Ampicillin" CDS 2622..3482 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3637..4256 /label="pBR322_origin" misc_feature 4472..4498 /label="lacI.C" promoter 4565..4594 /label="lac_promoter" misc_feature 4629..4647 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1321 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1381 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1441 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1501 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1561 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1621 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1681 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1741 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1801 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1861 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1921 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1981 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 2041 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 2101 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 2161 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2221 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2281 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2341 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2401 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2461 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2521 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2581 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2641 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2701 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2761 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2821 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2881 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2941 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 3001 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 3061 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 3121 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 3181 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3241 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3301 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3361 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3421 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3481 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3541 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3601 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3661 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3721 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3781 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3841 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3901 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3961 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 4021 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 4081 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 4141 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 4201 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4261 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4321 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4381 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4441 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4501 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4561 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4621 TTCACACAGG AAACAGCT // 1474 LOCUS ADDGENE_1474 4638 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_51 complete plasmid sequence KEYWORDS . SOURCE pPD93_51 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1474 at www.addgene.org FEATURES Location/Qualifiers source 1..4638 /organism="Addgene plasmid pPD93_51" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1374..1424 /label="SynIVS.L" promoter 2552..2580 /label="Amp_promoter" CDS 2622..3482 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2622..3482 /label="Ampicillin" /gene="Ampicillin" rep_origin 3637..4256 /label="pBR322_origin" misc_feature 4472..4498 /label="lacI.C" promoter 4565..4594 /label="lac_promoter" misc_feature 4629..4647 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CaCTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1321 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1381 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1441 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1501 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1561 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1621 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1681 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1741 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1801 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1861 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1921 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1981 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 2041 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 2101 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 2161 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2221 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2281 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2341 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2401 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2461 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2521 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2581 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2641 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2701 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2761 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2821 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2881 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2941 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 3001 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 3061 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 3121 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 3181 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3241 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3301 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3361 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3421 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3481 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3541 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3601 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3661 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3721 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3781 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3841 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3901 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3961 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 4021 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 4081 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 4141 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 4201 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4261 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4321 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4381 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4441 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4501 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4561 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4621 TTCACACAGG AAACAGCT // 1475 LOCUS ADDGENE_1475 4693 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_65 complete plasmid sequence KEYWORDS . SOURCE pPD93_65 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1475 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid pPD93_65" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFTYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65T" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1302..1352 /label="SynIVS.M" misc_feature 1429..1479 /label="SynIVS.L" promoter 2607..2635 /label="Amp_promoter" gene 2677..3537 /label="Ampicillin" /gene="Ampicillin" CDS 2677..3537 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3692..4311 /label="pBR322_origin" misc_feature 4527..4553 /label="lacI.C" promoter 4620..4649 /label="lac_promoter" misc_feature 4684..4702 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CaCTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT Tgtaagttta aactttctca 1321 tactaactaa cgcgctctat ttaaattttc agAATTCCAA CTGAGCGCCG GTCGCTACCA 1381 TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac 1441 tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG 1501 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 1561 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 1621 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 1681 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 1741 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 1801 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 1861 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 1921 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 1981 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 2041 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 2101 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 2161 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 2221 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 2281 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 2341 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 2401 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 2461 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 2521 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 2581 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 2641 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 2701 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 2761 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 2821 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 2881 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 2941 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 3001 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 3061 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 3121 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 3181 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 3241 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 3301 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 3361 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 3421 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 3481 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 3541 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 3601 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 3661 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 3721 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 3781 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 3841 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 3901 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 3961 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 4021 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 4081 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 4141 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 4201 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 4261 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 4321 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 4381 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 4441 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 4501 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 4561 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 4621 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 4681 ACAGGAAACA GCT // 1476 LOCUS ADDGENE_1476 6787 bp DNA circular SYN 01/03/2005 DEFINITION pPD93_97 complete plasmid sequence KEYWORDS . SOURCE pPD93_97 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1476 at www.addgene.org FEATURES Location/Qualifiers source 1..6787 /organism="Addgene plasmid pPD93_97" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2451..2492 /label="SynIVS.A" misc_feature 2526..2552 /label="SV40.NLS" CDS 2526..3437 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 2568..3428 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 2739..2789 /label="SynIVS.R" misc_feature 2811..2830 /label="gfp.S65C" misc_feature 2944..2994 /label="SynIVS.S" misc_feature 3159..3209 /label="SynIVS.T" misc_feature 3523..3573 /label="SynIVS.L" promoter 4701..4729 /label="Amp_promoter" CDS 4771..5631 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4771..5631 /label="Ampicillin" /gene="Ampicillin" rep_origin 5786..6405 /label="pBR322_origin" misc_feature 6621..6647 /label="lacI.C" promoter 6714..6743 /label="lac_promoter" misc_feature 6778..6796 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG gtatgtttcg 2461 aatgatacta acataacata gaacattttc agGAGGACCC TTGGAGGGTA CCGAGCTCAG 2521 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT AGAAAAAATG AGTAAAGGAG 2581 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC 2641 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA 2701 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac 2761 taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC TgTTATGGTG 2821 TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC AAGAGTGCCA 2881 TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA 2941 CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG 3001 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA 3061 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT 3121 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac 3181 taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG 3241 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC 3301 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA 3361 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 3421 ATGAACTATA CAAATAGCAT TCGTAGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 3481 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 3541 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 3601 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 3661 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 3721 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 3781 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 3841 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 3901 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 3961 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 4021 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 4081 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 4141 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 4201 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 4261 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 4321 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 4381 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 4441 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 4501 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 4561 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 4621 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 4681 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 4741 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 4801 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 4861 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 4921 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 4981 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 5041 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 5101 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 5161 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 5221 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 5281 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 5341 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 5401 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 5461 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 5521 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 5581 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 5641 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 5701 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 5761 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 5821 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 5881 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 5941 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 6001 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 6061 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 6121 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 6181 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 6241 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 6301 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 6361 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 6421 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 6481 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 6541 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 6601 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 6661 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 6721 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 6781 AACAGCT // 1477 LOCUS ADDGENE_1477 4693 bp DNA circular SYN 01/03/2005 DEFINITION pPD94_81 complete plasmid sequence KEYWORDS . SOURCE pPD94_81 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1477 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid pPD94_81" /mol_type="other DNA" CDS 1..912 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 1..27 /label="SV40.NLS" gene 43..903 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 214..264 /label="SynIVS.R" misc_feature 286..305 /label="gfp.S65C" misc_feature 419..469 /label="SynIVS.S" misc_feature 634..684 /label="SynIVS.T" misc_feature 926..976 /label="SynIVS.M" misc_feature 1053..1103 /label="SynIVS.L" promoter 2231..2259 /label="Amp_promoter" CDS 2301..3161 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2301..3161 /label="Ampicillin" /gene="Ampicillin" rep_origin 3316..3935 /label="pBR322_origin" misc_feature 4151..4177 /label="lacI.C" promoter 4244..4273 /label="lac_promoter" misc_feature 4308..4326 /label="M13r_primer" misc_feature 4363..4388 /label="unc54.-204" misc_feature 4578..4601 /label="unc54.5'" misc_feature 4619..4660 /label="SynIVS.A" ORIGIN 1 ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 61 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 121 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 181 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 241 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTgTTA TGGTGTTCAA 301 TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT TTTTCAAGAG TGCCATGCCC 361 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 421 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 481 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 541 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 601 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 661 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 721 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 781 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 841 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 901 CTATACAAAT AGCATTCGTA GAATTgtaag tttaaacttt ctcatactaa ctaacgcgct 961 ctatttaaat tttcagAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 1021 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 1081 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 1141 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 1201 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 1261 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 1321 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 1381 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 1441 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 1501 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 1561 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 1621 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 1681 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 1741 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 1801 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 1861 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 1921 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 1981 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 2041 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 2101 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 2161 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 2221 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 2281 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 2341 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 2401 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2461 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2521 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2581 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2641 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2701 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2761 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2821 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 2881 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 2941 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 3001 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 3061 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 3121 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 3181 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 3241 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 3301 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 3361 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 3421 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 3481 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 3541 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 3601 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 3661 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 3721 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 3781 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 3841 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 3901 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3961 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 4021 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 4081 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 4141 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 4201 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 4261 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 4321 ACCATGATTA CGCCAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCAATTCTTG 4381 AGACTTCCAC TATCTTCTCT GGCACCCTCC ACTACGCGTA GGCGTCTCTC GCTTCGTGTA 4441 TTCCCGGGAA GCCGGGTTCC CGTCTCTCCC GCCGCTGCCG CTGCCGCACA CAGCTTTACA 4501 CCTCGTAGAA TCCCCAAAGA GGGGCGTGGC TTGCGGGTGC CAACATCCTC CTGCCGAGGA 4561 AGAAGCAGGC ACTCATCACT CGCATCATCA ACCTCGGGAT TGGCCAAAGG ACCCAAAGGT 4621 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGAGGGTACC 4681 GAGCTCAGAA AAA // 1478 LOCUS ADDGENE_1478 3786 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_02 complete plasmid sequence KEYWORDS . SOURCE pPD95_02 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1478 at www.addgene.org FEATURES Location/Qualifiers source 1..3786 /organism="Addgene plasmid pPD95_02" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" CDS 31..1431 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTMITPSAQLTLTKGNKSWVPVEKMSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLV TTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*P YIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYL STQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPGDPLVLERPPPRWSSNSPY SESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNG EWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLPL GFRFSALRHLDPKKLD" promoter 56..75 /label="T3_promoter" gene 103..963 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 274..324 /label="SynIVS.R" misc_feature 346..365 /label="gfp.S65C" misc_feature 479..529 /label="SynIVS.S" misc_feature 694..744 /label="SynIVS.T" promoter complement(1027..1045) /label="T7_promoter" misc_feature 1065..1213 /label="lacZ_a" misc_feature complement(1056..1072) /label="M13f20_primer" rep_origin 1231..1537 /label="f1_origin" promoter 1730..1758 /label="Amp_promoter" gene 1800..2660 /label="Ampicillin" /gene="Ampicillin" CDS 1800..2660 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2815..3434 /label="pBR322_origin" misc_feature 3650..3676 /label="lacI.C" promoter 3743..3772 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA 121 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA 181 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT 241 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt taaacatata tatactaact 301 aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA CTTTCTgTTA TGGTGTTCAA 361 TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT TTTTCAAGAG TGCCATGCCC 421 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACgt 481 aagtttaaac agttcggtac taactaacca tacatattta aattttcagG TGCTGAAGTC 541 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA 601 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC 661 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt taaacatgat tttactaact 721 aactaatctg atttaaattt tcagAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC 781 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA 841 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA 901 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA 961 CTATACAAAT GCCCGGGGGA TCCACTAGTT CTAGAGCGGC CGCCACCGCG GTGGAGCTCC 1021 AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT 1081 GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC 1141 AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG 1201 AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 1261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 1321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 1381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 1441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 1501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 1561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 1621 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA GGTGGCACTT 1681 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 1741 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 1801 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 1861 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 1921 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 1981 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 2041 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 2101 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 2161 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 2221 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 2281 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 2341 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 2401 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 2461 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 2521 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 2581 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 2641 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 2701 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 2761 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 2821 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 2881 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 2941 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 3001 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 3061 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 3121 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 3181 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 3241 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 3301 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 3361 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 3421 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 3481 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 3541 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 3601 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 3661 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 3721 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 3781 TTGTGA // 1479 LOCUS ADDGENE_1479 7229 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_03 complete plasmid sequence KEYWORDS . SOURCE pPD95_03 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1479 at www.addgene.org FEATURES Location/Qualifiers source 1..7229 /organism="Addgene plasmid pPD95_03" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" CDS 137..850 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 137..163 /label="SV40.NLS" gene 254..3807 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 388..438 /label="SynIVS.B" CDS 889..3810 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1066..1116 /label="SynIVS.D" misc_feature 1507..1557 /label="SynIVS.E" misc_feature 1814..1864 /label="SynIVS.F" misc_feature 2095..2145 /label="SynIVS.G" misc_feature 2382..2432 /label="SynIVS.H" misc_feature 2748..2798 /label="SynIVS.I" misc_feature 3059..3109 /label="SynIVS.J" misc_feature 3465..3515 /label="SynIVS.K" misc_feature 3831..3881 /label="SynIVS.L" promoter 5009..5037 /label="Amp_promoter" gene 5079..5939 /label="Ampicillin" /gene="Ampicillin" CDS 5079..5939 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6094..6713 /label="pBR322_origin" misc_feature 6929..6955 /label="lacI.C" promoter 7022..7051 /label="lac_promoter" misc_feature 7086..7104 /label="M13r_primer" misc_feature 7118..7168 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 181 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 241 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 301 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 361 CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact aactaacttg 421 ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA 481 AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC 541 CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA CCTATCCCAT 601 TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT 661 TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTTAA 721 CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg taagtttaat 781 taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT GCCGTCTGAA 841 TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC 901 TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT 961 GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC 1021 TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag tttaaacagg 1081 atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT GCGTGACTAC 1141 CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG CACCGCGCCT 1201 TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC ACTACGTCTG 1261 AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG TGCGGTGGTT 1321 GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT CGGTTTCCGC 1381 GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT GATTCGAGGC 1441 GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA GACGATGGTG 1501 CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa ttttcagATC 1561 CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC GAACCATCCG 1621 CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC CAATATTGAA 1681 ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT ACCGGCGATG 1741 AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT GATCATCTGG 1801 TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc tttgaaattt 1861 tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 1921 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 1981 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 2041 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG CGAGgtaagt 2101 ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC tCACGCGATG 2161 GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA TCCCCGTTTA 2221 CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA TGAAAACGGC 2281 AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG CCAGTTCTGT 2341 ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta aacaataacc 2401 tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA CCAGCAGCAG 2461 TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT 2521 CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC 2581 GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA 2641 CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG 2701 ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta agtttaaaca 2761 agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA CCTCAGTGTG 2821 ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC 2881 ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG 2941 TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG 3001 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGgt 3061 aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA ACGCTGGAAG 3121 GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT 3181 GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC 3241 AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA 3301 GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA 3361 GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT 3421 GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt ttaaacttga 3481 tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT CCCGAGCGAA 3541 AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG GCGCGGCGAC 3601 TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG CCATCGCCAT 3661 CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT GGGGATTGGT 3721 GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC CGGTCGCTAC 3781 CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA gtaagtttaa 3841 actgagttct actaactaac gagtaatatt taaattttca gCATCTCGCG CCCGTGCCTC 3901 TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC TGTGCTCCCA 3961 CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT TAGCTTCTTT 4021 TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT CGTAATAAAA 4081 AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA AAATCTACAC 4141 AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG AAACATCATA 4201 GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG ACCGCAATTT 4261 TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG TGAAAAAGTT 4321 TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT TTTCCTGCTT 4381 TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT TTTTCTTGCT 4441 AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT TGGGTTTGAG 4501 GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC TATGGGGTTT 4561 TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC CTACTAGTCG 4621 GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 4681 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 4741 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 4801 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 4861 AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG 4921 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 4981 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 5041 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 5101 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 5161 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 5221 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 5281 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 5341 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 5401 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 5461 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 5521 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 5581 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 5641 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 5701 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 5761 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 5821 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 5881 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 5941 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 6001 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 6061 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 6121 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 6181 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 6241 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 6301 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 6361 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 6421 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 6481 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 6541 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 6601 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 6661 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 6721 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 6781 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 6841 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 6901 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 6961 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 7021 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC 7081 ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTgta agtttaaaca tgatcttact 7141 aactaactat tctcatttaa attttcagAG CTTAAAAATG GCTGAAATCA CTCACAACGA 7201 TGGATACGCT AACAACTTGG AAATGAAAT // 1480 LOCUS ADDGENE_1480 7233 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_07 complete plasmid sequence KEYWORDS . SOURCE pPD95_07 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1480 at www.addgene.org FEATURES Location/Qualifiers source 1..7233 /organism="Addgene plasmid pPD95_07" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" misc_feature 141..167 /label="SV40.NLS" CDS 141..854 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 258..3811 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 392..442 /label="SynIVS.B" CDS 893..3814 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1070..1120 /label="SynIVS.D" misc_feature 1511..1561 /label="SynIVS.E" misc_feature 1818..1868 /label="SynIVS.F" misc_feature 2099..2149 /label="SynIVS.G" misc_feature 2386..2436 /label="SynIVS.H" misc_feature 2752..2802 /label="SynIVS.I" misc_feature 3063..3113 /label="SynIVS.J" misc_feature 3469..3519 /label="SynIVS.K" misc_feature 3835..3885 /label="SynIVS.L" promoter 5013..5041 /label="Amp_promoter" CDS 5083..5943 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5083..5943 /label="Ampicillin" /gene="Ampicillin" rep_origin 6098..6717 /label="pBR322_origin" misc_feature 6933..6959 /label="lacI.C" promoter 7026..7055 /label="lac_promoter" misc_feature 7090..7108 /label="M13r_primer" misc_feature 7122..7172 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTGGGTG 181 AAGACCAGAA ACAGCACCTC GAACTGAGCC GCGATATTGC CCAGCGTTTC AACGCGCTGT 241 ATGGCGAGAT CGATCCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 301 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 361 GCACCGATCG CCCTTCCCAA CAGTTGCGaA Ggtaagttta aacagatcca tactaactaa 421 cttgttctga cataattttc agCtTGAATG GCGAATGGCG CTTTGCCTGG TTTCCGGCAC 481 CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC TGAGGCCGAT ACTGTCGTCG 541 TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT CTACACCAAC GTGACCTATC 601 CCATTACGGT CAATCCGCCG TTTGTTCCCA CGGAGAATCC GACGGGTTGT TACTCGCTCA 661 CATTTAATGT TGATGAAAGC TGGCTACAGG AAGGCCAGAC GCGAATTATT TTTGATGGCG 721 TTAACTCGGC GTTTCATCTG TGGTGCAACG GGCGCTGGGT CGGTTACGGC CAGgtaagtt 781 taattaagtt gataCTAACT AACAAGATCT GATTAATTTT CagGACAGTC GTTTGCCGTC 841 TGAATTTGAC CTGAGCGCAT TTTTACGCGC CGGAGAAAAC CGCCTCGCGG TGATGGTGCT 901 GCGCTGGAGT GACGGCAGTT ATCTGGAAGA TCAGGATATG TGGCGGATGA GCGGCATTTT 961 CCGTGACGTC TCGTTGCTGC ATAAACCGAC TACACAAATC AGCGATTTCC ATGTTGCCAC 1021 TCGCTTTAAT GATGATTTCt ccCGCGCTGT ACTGGAGGCT GAAGTcCAGg taagtttaaa 1081 caggatctta ctaactaaca tgctaacact gaattttcag ATGTGCGGCG AGTTGCGTGA 1141 CTACCTACGG GTAACAGTTT CTTTATGGCA GGGTGAAACG CAGGTCGCCA GCGGCACCGC 1201 GCCTTTCGGC GGTGAAATTA TCGATGAGCG TGGTGGTTAT GCCGATCGCG TCACACTACG 1261 TCTGAACGTC GAAAACCCGA AACTGTGGAG CGCCGAAATC CCGAATCTCT ATCGTGCGGT 1321 GGTTGAACTG CACACCGCCG ACGGCACGCT GATTGAAGCA GAAGCCTGCG ATGTCGGTTT 1381 CCGCGAGGTG CGGATTGAAA ATGGTCTGCT GCTGCTGAAC GGCAAGCCGT TGCTGATTCG 1441 AGGCGTTAAC CGTCACGAGC ATCATCCTCT GCATGGTCAG GTCATGGATG AGCAGACGAT 1501 GGTGCAGGAT gtaagtttaa actattcgtt actaactaac tttaaacatt taaattttca 1561 gATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA 1621 TCCGCTGTGG TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT 1681 TGAAACCCAC GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC 1741 GATGAGCGAA CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT 1801 CTGGTCGCTG GGGAATGgta agtttaaaca gttgaatact aactaacgga gatctttgaa 1861 attttcagAA TCAGGCCACG GCGCTAATCA CGACGCGCTG TATCGCTGGA TCAAATCTGT 1921 CGATCCTTCC CGCCCGGTGC AGTATGAAGG CGGCGGAGCC GACACCACGG CCACCGATAT 1981 TATTTGCCCG ATGTACGCGC GCGTGGATGA AGACCAGCCC TTCCCGGCTG TGCCGAAATG 2041 GTCCATCAAA AAATGGCTTT CGCTACCTGG AGAGACGCGC CCGCTGATtC TTTGCGAGgt 2101 aagtttaaac agaactctac taactaacac attagatcct aattttcagT ACGCtCACGC 2161 GATGGGcAAC AGTCTTGGCG GTTTCGCTAA ATACTGGCAG GCGTTTCGTC AGTATCCCCG 2221 TTTACAGGGC GGCTTCGTCT GGGACTGGGT GGATCAGTCG CTGATTAAAT ATGATGAAAA 2281 CGGCAACCCG TGGTCGGCTT ACGGCGGTGA TTTTGGCGAT ACGCCGAACG ATCGCCAGTT 2341 CTGTATGAAC GGTCTGGTCT TTGCCGACCG CACGCCGCAT CCAGCgtaag tttaaacaat 2401 aacctactaa ctaacgtaga taatttaaat tttcagGCTG ACGGAAGCAA AACACCAGCA 2461 GCAGTTTTTC CAGTTCCGTT TATCCGGGCA AACCATCGAA GTGACCAGCG AATACCTGTT 2521 CCGTCATAGC GATAACGAGC TCCTGCACTG GATGGTGGCG CTGGATGGTA AGCCGCTGGC 2581 AAGCGGTGAA GTGCCTCTGG ATGTCGCTCC ACAAGGTAAA CAGTTGATTG AACTGCCTGA 2641 ACTACCGCAG CCGGAGAGCG CCGGGCAACT CTGGCTCACA GTACGCGTAG TGCAACCGAA 2701 CGCGACCGCA TGGTCAGAAG CCGGGCACAT CAGCGCaTGG CAGCAGTGGA Ggtaagttta 2761 aacaagatcc tactaactaa ctctacattg atgaattttc agACTGGCGG AAAACCTCAG 2821 TGTGACGCTC CCCGCCGCGT CCCACGCCAT CCCGCATCTG ACCACCAGCG AAATGGATTT 2881 TTGCATCGAG CTGGGTAATA AGCGTTGGCA ATTTAACCGC CAGTCAGGCT TTCTTTCACA 2941 GATGTGGATT GGCGATAAAA AACAACTGCT GACGCCGCTG CGCGATCAGT TCACCCGTGC 3001 ACCGCTGGAT AACGACATTG GCGTAAGTGA AGCGACCCGC ATTGACCCTA ACGCCTGGGT 3061 CGgtaagttt aaacaaagtt gtactaacta acgaagatct tgataatttt cagAACGCTG 3121 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 3181 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 3241 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 3301 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 3361 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 3421 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTAgt aagtttaaac 3481 ttgatagtac taactaacat gtttcattta aattttcagT ACCCCGTACG TCTTCCCGAG 3541 CGAAAACGGT CTGCGCTGCG GGACGCGCGA ATTGAATTAT GGCCCACACC AGTGGCGCGG 3601 CGACTTCCAG TTCAACATCA GCCGCTACAG TCAACAGCAA CTGATGGAAA CCAGCCATCG 3661 CCATCTGCTG CACGCGGAAG AAGGCACATG GCTGAATATC GACGGTTTCC ATATGGGGAT 3721 TGGTGGCGAC GACTCCTGGA GCCCGTCAGT ATCGGCGGAA TTCCAACTGA GCGCCGGTCG 3781 CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt 3841 ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG 3901 CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT 3961 CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT 4021 CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT 4081 AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT 4141 ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT 4201 CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA 4261 ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA 4321 AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT 4381 GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT 4441 TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT 4501 TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG 4561 GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA 4621 GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 4681 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 4741 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 4801 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 4861 GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT 4921 AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC 4981 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 5041 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC 5101 CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA 5161 ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA 5221 CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG 5281 ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA 5341 GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC 5401 ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC 5461 ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA 5521 ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG 5581 CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA 5641 ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA 5701 GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC 5761 TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA 5821 CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA 5881 ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG 5941 TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA 6001 TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT 6061 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT 6121 CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG 6181 GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA 6241 GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC 6301 TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 6361 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG 6421 CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC 6481 GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG 6541 GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA 6601 GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 6661 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC 6721 TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC 6781 CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC 6841 CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA 6901 CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC 6961 TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC 7021 CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA 7081 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct 7141 tactaactaa ctattctcat ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA 7201 ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1481 LOCUS ADDGENE_1481 7227 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_10 complete plasmid sequence KEYWORDS . SOURCE pPD95_10 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1481 at www.addgene.org FEATURES Location/Qualifiers source 1..7227 /organism="Addgene plasmid pPD95_10" /mol_type="other DNA" CDS 9..848 /label="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAA HPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVP ESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFN VDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRL NLT" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" misc_feature 135..161 /label="SV40.NLS" gene 252..3805 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 386..436 /label="SynIVS.B" CDS 887..3808 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1064..1114 /label="SynIVS.D" misc_feature 1505..1555 /label="SynIVS.E" misc_feature 1812..1862 /label="SynIVS.F" misc_feature 2093..2143 /label="SynIVS.G" misc_feature 2380..2430 /label="SynIVS.H" misc_feature 2746..2796 /label="SynIVS.I" misc_feature 3057..3107 /label="SynIVS.J" misc_feature 3463..3513 /label="SynIVS.K" misc_feature 3829..3879 /label="SynIVS.L" promoter 5007..5035 /label="Amp_promoter" gene 5077..5937 /label="Ampicillin" /gene="Ampicillin" CDS 5077..5937 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6092..6711 /label="pBR322_origin" misc_feature 6927..6953 /label="lacI.C" promoter 7020..7049 /label="lac_promoter" misc_feature 7084..7102 /label="M13r_primer" misc_feature 7116..7166 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGTG GGTGAAGACC 181 AGAAACAGCA CCTCGAACTG AGCCGCGATA TTGCCCAGCG TTTCAACGCG CTGTATGGCG 241 AGATCGATCC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 301 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 361 ATCGCCCTTC CCAACAGTTG CGaAGgtaag tttaaacaga tccatactaa ctaacttgtt 421 ctgacataat tttcagCtTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG GCACCAGAAG 481 CGGTGCCGGA AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC GTCGTCCCCT 541 CAAACTGGCA GATGCACGGT TACGATGCGC CCATCTACAC CAACGTGACC TATCCCATTA 601 CGGTCAATCC GCCGTTTGTT CCCACGGAGA ATCCGACGGG TTGTTACTCG CTCACATTTA 661 ATGTTGATGA AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT GGCGTTAACT 721 CGGCGTTTCA TCTGTGGTGC AACGGGCGCT GGGTCGGTTA CGGCCAGgta agtttaatta 781 agttgataCT AACTAACAAG ATCTGATTAA TTTTCagGAC AGTCGTTTGC CGTCTGAATT 841 TGACCTGAGC GCATTTTTAC GCGCCGGAGA AAACCGCCTC GCGGTGATGG TGCTGCGCTG 901 GAGTGACGGC AGTTATCTGG AAGATCAGGA TATGTGGCGG ATGAGCGGCA TTTTCCGTGA 961 CGTCTCGTTG CTGCATAAAC CGACTACACA AATCAGCGAT TTCCATGTTG CCACTCGCTT 1021 TAATGATGAT TTCtccCGCG CTGTACTGGA GGCTGAAGTc CAGgtaagtt taaacaggat 1081 cttactaact aacatgctaa cactgaattt tcagATGTGC GGCGAGTTGC GTGACTACCT 1141 ACGGGTAACA GTTTCTTTAT GGCAGGGTGA AACGCAGGTC GCCAGCGGCA CCGCGCCTTT 1201 CGGCGGTGAA ATTATCGATG AGCGTGGTGG TTATGCCGAT CGCGTCACAC TACGTCTGAA 1261 CGTCGAAAAC CCGAAACTGT GGAGCGCCGA AATCCCGAAT CTCTATCGTG CGGTGGTTGA 1321 ACTGCACACC GCCGACGGCA CGCTGATTGA AGCAGAAGCC TGCGATGTCG GTTTCCGCGA 1381 GGTGCGGATT GAAAATGGTC TGCTGCTGCT GAACGGCAAG CCGTTGCTGA TTCGAGGCGT 1441 TAACCGTCAC GAGCATCATC CTCTGCATGG TCAGGTCATG GATGAGCAGA CGATGGTGCA 1501 GGATgtaagt ttaaactatt cgttactaac taactttaaa catttaaatt ttcagATCCT 1561 GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT 1621 GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC 1681 CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG 1741 CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC 1801 GCTGGGGAAT Ggtaagttta aacagttgaa tactaactaa cggagatctt tgaaattttc 1861 agAATCAGGC CACGGCGCTA ATCACGACGC GCTGTATCGC TGGATCAAAT CTGTCGATCC 1921 TTCCCGCCCG GTGCAGTATG AAGGCGGCGG AGCCGACACC ACGGCCACCG ATATTATTTG 1981 CCCGATGTAC GCGCGCGTGG ATGAAGACCA GCCCTTCCCG GCTGTGCCGA AATGGTCCAT 2041 CAAAAAATGG CTTTCGCTAC CTGGAGAGAC GCGCCCGCTG ATtCTTTGCG AGgtaagttt 2101 aaacagaact ctactaacta acacattaga tcctaatttt cagTACGCtC ACGCGATGGG 2161 cAACAGTCTT GGCGGTTTCG CTAAATACTG GCAGGCGTTT CGTCAGTATC CCCGTTTACA 2221 GGGCGGCTTC GTCTGGGACT GGGTGGATCA GTCGCTGATT AAATATGATG AAAACGGCAA 2281 CCCGTGGTCG GCTTACGGCG GTGATTTTGG CGATACGCCG AACGATCGCC AGTTCTGTAT 2341 GAACGGTCTG GTCTTTGCCG ACCGCACGCC GCATCCAGCg taagtttaaa caataaccta 2401 ctaactaacg tagataattt aaattttcag GCTGACGGAA GCAAAACACC AGCAGCAGTT 2461 TTTCCAGTTC CGTTTATCCG GGCAAACCAT CGAAGTGACC AGCGAATACC TGTTCCGTCA 2521 TAGCGATAAC GAGCTCCTGC ACTGGATGGT GGCGCTGGAT GGTAAGCCGC TGGCAAGCGG 2581 TGAAGTGCCT CTGGATGTCG CTCCACAAGG TAAACAGTTG ATTGAACTGC CTGAACTACC 2641 GCAGCCGGAG AGCGCCGGGC AACTCTGGCT CACAGTACGC GTAGTGCAAC CGAACGCGAC 2701 CGCATGGTCA GAAGCCGGGC ACATCAGCGC aTGGCAGCAG TGGAGgtaag tttaaacaag 2761 atcctactaa ctaactctac attgatgaat tttcagACTG GCGGAAAACC TCAGTGTGAC 2821 GCTCCCCGCC GCGTCCCACG CCATCCCGCA TCTGACCACC AGCGAAATGG ATTTTTGCAT 2881 CGAGCTGGGT AATAAGCGTT GGCAATTTAA CCGCCAGTCA GGCTTTCTTT CACAGATGTG 2941 GATTGGCGAT AAAAAACAAC TGCTGACGCC GCTGCGCGAT CAGTTCACCC GTGCACCGCT 3001 GGATAACGAC ATTGGCGTAA GTGAAGCGAC CCGCATTGAC CCTAACGCCT GGGTCGgtaa 3061 gtttaaacaa agttgtacta actaacgaag atcttgataa ttttcagAAC GCTGGAAGGC 3121 GGCGGGCCAT TACCAGGCCG AAGCAGCGTT GTTGCAGTGC ACGGCAGATA CACTTGCTGA 3181 TGCGGTGCTG ATTACGACCG CTCACGCGTG GCAGCATCAG GGGAAAACCT TATTTATCAG 3241 CCGGAAAACC TACCGGATTG ATGGTAGTGG TCAAATGGCG ATTACCGTTG ATGTTGAAGT 3301 GGCGAGCGAT ACACCGCATC CGGCGCGGAT TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC 3361 AGAGCGGGTA AACTGGCTCG GATTAGGGCC GCAAGAAAAC TATCCCGACC GCCTTACTGC 3421 CGCCTGTTTT GACCGCTGGG ATCTGCCATT GTCAGACATG TAgtaagttt aaacttgata 3481 gtactaacta acatgtttca tttaaatttt cagTACCCCG TACGTCTTCC CGAGCGAAAA 3541 CGGTCTGCGC TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT 3601 CCAGTTCAAC ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT 3661 GCTGCACGCG GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG 3721 CGACGACTCC TGGAGCCCGT CAGTATCGGC GGAATTCCAA CTGAGCGCCG GTCGCTACCA 3781 TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt aagtttaaac 3841 tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC CGTGCCTCTG 3901 ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC 3961 CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA 4021 AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG 4081 TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA 4141 TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA 4201 AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT 4261 ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT 4321 GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT 4381 GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA 4441 AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC 4501 TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT 4561 GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC 4621 CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC 4681 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC 4741 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC 4801 AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA 4861 AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC 4921 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 4981 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC 5041 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 5101 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 5161 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 5221 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 5281 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA 5341 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 5401 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 5461 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT 5521 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 5581 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG 5641 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 5701 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 5761 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 5821 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 5881 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG 5941 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA 6001 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT 6061 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT 6121 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 6181 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG 6241 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA 6301 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT 6361 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG 6421 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 6481 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC 6541 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA 6601 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT 6661 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA 6721 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 6781 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG 6841 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT 6901 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 6961 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT 7021 TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC 7081 ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag tttaaacatg atcttactaa 7141 ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC TGAAATCACT CACAACGATG 7201 GATACGCTAA CAACTTGGAA ATGAAAT // 1482 LOCUS ADDGENE_1482 6434 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_11 complete plasmid sequence KEYWORDS . SOURCE pPD95_11 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1482 at www.addgene.org FEATURES Location/Qualifiers source 1..6434 /organism="Addgene plasmid pPD95_11" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 137..163 /label="SV40.NLS" CDS 137..850 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 254..3807 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 388..438 /label="SynIVS.B" CDS 889..3810 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1066..1116 /label="SynIVS.D" misc_feature 1507..1557 /label="SynIVS.E" misc_feature 1814..1864 /label="SynIVS.F" misc_feature 2095..2145 /label="SynIVS.G" misc_feature 2382..2432 /label="SynIVS.H" misc_feature 2748..2798 /label="SynIVS.I" misc_feature 3059..3109 /label="SynIVS.J" misc_feature 3465..3515 /label="SynIVS.K" promoter 4214..4242 /label="Amp_promoter" CDS 4284..5144 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4284..5144 /label="Ampicillin" /gene="Ampicillin" rep_origin 5299..5918 /label="pBR322_origin" misc_feature 6134..6160 /label="lacI.C" promoter 6227..6256 /label="lac_promoter" misc_feature 6291..6309 /label="M13r_primer" misc_feature 6323..6373 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TGGGTGAAGA 181 CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG CGCTGTATGG 241 CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT 301 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC 361 CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact aactaacttg 421 ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA 481 AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC 541 CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA CCTATCCCAT 601 TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT 661 TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTTAA 721 CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg taagtttaat 781 taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT GCCGTCTGAA 841 TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC 901 TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT 961 GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC 1021 TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag tttaaacagg 1081 atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT GCGTGACTAC 1141 CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG CACCGCGCCT 1201 TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC ACTACGTCTG 1261 AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG TGCGGTGGTT 1321 GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT CGGTTTCCGC 1381 GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT GATTCGAGGC 1441 GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA GACGATGGTG 1501 CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa ttttcagATC 1561 CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC GAACCATCCG 1621 CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC CAATATTGAA 1681 ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT ACCGGCGATG 1741 AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT GATCATCTGG 1801 TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc tttgaaattt 1861 tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA ATCTGTCGAT 1921 CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC CGATATTATT 1981 TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC GAAATGGTCC 2041 ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG CGAGgtaagt 2101 ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC tCACGCGATG 2161 GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA TCCCCGTTTA 2221 CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA TGAAAACGGC 2281 AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG CCAGTTCTGT 2341 ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta aacaataacc 2401 tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA CCAGCAGCAG 2461 TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT 2521 CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC 2581 GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA 2641 CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG 2701 ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta agtttaaaca 2761 agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA CCTCAGTGTG 2821 ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC 2881 ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG 2941 TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG 3001 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGgt 3061 aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA ACGCTGGAAG 3121 GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA TACACTTGCT 3181 GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC CTTATTTATC 3241 AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT TGATGTTGAA 3301 GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT GGCGCAGGTA 3361 GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA CCGCCTTACT 3421 GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt ttaaacttga 3481 tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT CCCGAGCGAA 3541 AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG GCGCGGCGAC 3601 TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG CCATCGCCAT 3661 CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT GGGGATTGGT 3721 GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC CGGTCGCTAC 3781 CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGCCTACT AGTCGGCCGT ACGGGCCCTT 3841 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 3901 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 3961 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4021 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4081 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 4141 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 4201 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 4261 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 4321 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 4381 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 4441 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 4501 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 4561 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 4621 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 4681 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 4741 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 4801 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 4861 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 4921 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 4981 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 5041 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 5101 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 5161 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 5221 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 5281 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 5341 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 5401 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 5461 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 5521 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 5581 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 5641 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 5701 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 5761 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 5821 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 5881 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 5941 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 6001 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 6061 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 6121 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 6181 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 6241 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 6301 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 6361 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 6421 CTTGGAAATG AAAT // 1483 LOCUS ADDGENE_1483 8838 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_16 complete plasmid sequence KEYWORDS . SOURCE pPD95_16 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1483 at www.addgene.org FEATURES Location/Qualifiers source 1..8838 /organism="Addgene plasmid pPD95_16" /mol_type="other DNA" misc_feature 25..49 /label="glp1.delta-1635" misc_feature 1634..1661 /label="glp1.Tsa" CDS 1746..2459 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 1746..1772 /label="SV40.NLS" gene 1863..5416 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1997..2047 /label="SynIVS.B" CDS 2498..5419 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2675..2725 /label="SynIVS.D" misc_feature 3116..3166 /label="SynIVS.E" misc_feature 3423..3473 /label="SynIVS.F" misc_feature 3704..3754 /label="SynIVS.G" misc_feature 3991..4041 /label="SynIVS.H" misc_feature 4357..4407 /label="SynIVS.I" misc_feature 4668..4718 /label="SynIVS.J" misc_feature 5074..5124 /label="SynIVS.K" misc_feature 5440..5490 /label="SynIVS.L" promoter 6618..6646 /label="Amp_promoter" gene 6688..7548 /label="Ampicillin" /gene="Ampicillin" CDS 6688..7548 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7703..8322 /label="pBR322_origin" misc_feature 8538..8564 /label="lacI.C" promoter 8631..8660 /label="lac_promoter" misc_feature 8695..8713 /label="M13r_primer" misc_feature 8727..8777 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAG TATGAGAAAC AACATTAACA ATGTTTTGGG 61 AGAAACATGT AGTTTTGTAC GTCCGGTCCA GTGAGAGTTT TCAACTTATT TTGGAAGAAA 121 TTTTTTTTTG ACAAAATTTT GCAATTTCCC ACGCAGAAAT ACGGTATGCC CCGTATCTCG 181 CCACGAACAT AATATTTTAA ATTGACGGAA AGGTGTGCGC CTTTAAGAAG CACAGTAACC 241 ATTTTCCGGT TTTTTTTTTG AAATTGCCTT TGATTTTTAC TTTTTTCTCC CAAAAACGAG 301 AAATTTAATA TAATCCATCA AAATCTTTGT GAACGAGAGA AATATAGTGA AAAACTCAAA 361 GAAAATTGCG AAATGGATTA CTGTACTCCA TAAAGGCGCA CATCTTTTCG AAAAATCGTG 421 AAAAATTGCA TCTGACCAAC AGTTTTGCGT GGAATCTTCC TAGATTCAAA TTTCCTATGA 481 GTCATAAAAC TATCATTTTA TCGGACAATA ACTACACGTG TACCTTGTTT CCATATGTTC 541 TACCAATATA ATTTGTGTTC ATATGTGGCC AGGTACGGGA AATTAGAACA CGGACTGATT 601 TAGGAAGGTT CAAATTCGAG AACAATGGAC GACTTGCTGC ACGAAAACGT GGATTATTCT 661 GAAACCGCCT ATGTGATCAC TATCGATTTA GCACTTCGGT TTTCTTTTTC CGTTTTCCTT 721 TATATTCCGT ATTGTATGCC GACAATAACG CTTTTTCTTT GTATAAACAT TTCCAGAAGC 781 AGAGCAAATT TGTTTATTCC AGAATTGACT GAATATTGAA ATAATGTTTA CAGACATACT 841 GTAGATTTTT TTCTATTCAA ACATGAATAC AGTTTTTCGT ATTTTTAATC TTCAGATAAG 901 TTTTGTGTGT GTTTTTGAGA ATAATATTTA AAAAAGATAA GCAAATATCA GAACTACGGT 961 AAGCGAAGGG GTACGGTCGT CAATTGCGGA CTTCCGAGCT GTGCACGGCG CCAAAATTTA 1021 TTTTGAGTTT TAAAATGATT TTCTTTGTTT TTTTTTCTTT TTTTGACACG ATAAGAACAT 1081 TTTTCTGTAT TTCTAATATT TTAAATGAGA TCGTAATAAG TTTATTTAGC TCCATCACAT 1141 TATTTTCGGA ATATTCATCG CTTGAAAACG GCGTAAAATA GGTTCCAAGT AAATCATTGC 1201 AAACAAATCT CCGTTTATCG TTCTAACAAA GTTTTTCGTT CACTTTCTTA TGTAAATCGT 1261 TTTCCCCACT TCTTCTTCAC GGGAATGATC AATTGAAAGG CCATAAATTG GTTGTTTTCT 1321 CCACATTTCG CAACCAACAT GCAGCTGTCG GATAGGCAAT AAAATAATGT AATCAATGAG 1381 TTTTCCGATT TATCCTAACA GTTCTTTTTC TAGTGTTCCT ATTCGAAAAA AGTACTGTTA 1441 TCCTTCTTTT TGGACCGCCC ATCTCAGGTG TCAAGACAAT CGCAACACAC TCAGAAAATG 1501 GGCGGAGTAT GCCACCCGGC CTTCATTCGT CGCTCCTTGT CTTCTCCATT TTCGTTTTTT 1561 TCCTGTTCTC CGTTCTTTTT TTGTACTTTT GTGATAATGC TGAAGTGTTC TTTTTTTTCG 1621 CTTAACCGCA TCTCTTGATT TAATTCATAT TTTCTTTCAG ATTCATaTAC AATAACACCA 1681 AGAGCTGCTC TAACAACTAA TAATAACAGA ATCTAGAGGA TCCCCGGGTA CCGAGCTCAG 1741 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC CAGAAACAGC 1801 ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC GAGATCGATC 1861 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 1921 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT 1981 CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt tctgacataa 2041 ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA GCGGTGCCGG 2101 AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC TCAAACTGGC 2161 AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT ACGGTCAATC 2221 CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT AATGTTGATG 2281 AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC TCGGCGTTTC 2341 ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt aagttgataC 2401 TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT TTGACCTGAG 2461 CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT GGAGTGACGG 2521 CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG ACGTCTCGTT 2581 GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT TTAATGATGA 2641 TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga tcttactaac 2701 taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 2761 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 2821 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 2881 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 2941 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 3001 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 3061 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag 3121 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA 3181 GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC 3241 GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT 3301 GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT 3361 AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA 3421 TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt cagAATCAGG 3481 CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC CTTCCCGCCC 3541 GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT GCCCGATGTA 3601 CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA TCAAAAAATG 3661 GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt taaacagaac 3721 tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG GcAACAGTCT 3781 TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC AGGGCGGCTT 3841 CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA ACCCGTGGTC 3901 GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA TGAACGGTCT 3961 GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct actaactaac 4021 gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT TTTTCCAGTT 4081 CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC ATAGCGATAA 4141 CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC 4201 TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC CGCAGCCGGA 4261 GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA CCGCATGGTC 4321 AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa gatcctacta 4381 actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC 4441 CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA TCGAGCTGGG 4501 TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT GGATTGGCGA 4561 TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC TGGATAACGA 4621 CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta agtttaaaca 4681 aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG CGGCGGGCCA 4741 TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT 4801 GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC 4861 CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA 4921 TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT 4981 AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT 5041 TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat agtactaact 5101 aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA ACGGTCTGCG 5161 CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT TCCAGTTCAA 5221 CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC TGCTGCACGC 5281 GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG GCGACGACTC 5341 CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC ATTACCAACT 5401 TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa ctgagttcta 5461 ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT GACTTCTAAG 5521 TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC CCCCTATTTT 5581 TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC 5641 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA 5701 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG 5761 TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG AAAAAACCGC 5821 ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT TATTTCTTCG 5881 CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT TGGAGTATTT 5941 TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT TGCTTTTTGG 6001 GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA AAATCACAAG 6061 TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA 6121 GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT 6181 GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG CCGTACGGGC 6241 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG 6301 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT 6361 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC 6421 TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA 6481 TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA 6541 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 6601 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 6661 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 6721 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 6781 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 6841 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 6901 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 6961 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 7021 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 7081 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 7141 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 7201 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 7261 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 7321 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 7381 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 7441 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 7501 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 7561 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 7621 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 7681 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 7741 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 7801 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 7861 ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 7921 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 7981 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 8041 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 8101 CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 8161 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 8221 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 8281 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 8341 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 8401 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 8461 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 8521 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 8581 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 8641 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC 8701 AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta actaactatt 8761 ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT GGATACGCTA 8821 ACAACTTGGA AATGAAAT // 1484 LOCUS ADDGENE_1484 7610 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_18 complete plasmid sequence KEYWORDS . SOURCE pPD95_18 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1484 at www.addgene.org FEATURES Location/Qualifiers source 1..7610 /organism="Addgene plasmid pPD95_18" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..1231 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 635..4188 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 769..819 /label="SynIVS.B" CDS 1270..4191 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1447..1497 /label="SynIVS.D" misc_feature 1888..1938 /label="SynIVS.E" misc_feature 2195..2245 /label="SynIVS.F" misc_feature 2476..2526 /label="SynIVS.G" misc_feature 2763..2813 /label="SynIVS.H" misc_feature 3129..3179 /label="SynIVS.I" misc_feature 3440..3490 /label="SynIVS.J" misc_feature 3846..3896 /label="SynIVS.K" misc_feature 4212..4262 /label="SynIVS.L" promoter 5390..5418 /label="Amp_promoter" CDS 5460..6320 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5460..6320 /label="Ampicillin" /gene="Ampicillin" rep_origin 6475..7094 /label="pBR322_origin" misc_feature 7310..7336 /label="lacI.C" promoter 7403..7432 /label="lac_promoter" misc_feature 7467..7485 /label="M13r_primer" misc_feature 7499..7549 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGaAGgt aagtttaaac 781 agatccatac taactaactt gttctgacat aattttcagC tTGAATGGCG AATGGCGCTT 841 TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA 901 GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC GGTTACGATG CGCCCATCTA 961 CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT GTTCCCACGG AGAATCCGAC 1021 GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG CTACAGGAAG GCCAGACGCG 1081 AATTATTTTT GATGGCGTTA ACTCGGCGTT TCATCTGTGG TGCAACGGGC GCTGGGTCGG 1141 TTACGGCCAG gtaagtttaa ttaagttgat aCTAACTAAC AAGATCTGAT TAATTTTCag 1201 GACAGTCGTT TGCCGTCTGA ATTTGACCTG AGCGCATTTT TACGCGCCGG AGAAAACCGC 1261 CTCGCGGTGA TGGTGCTGCG CTGGAGTGAC GGCAGTTATC TGGAAGATCA GGATATGTGG 1321 CGGATGAGCG GCATTTTCCG TGACGTCTCG TTGCTGCATA AACCGACTAC ACAAATCAGC 1381 GATTTCCATG TTGCCACTCG CTTTAATGAT GATTTCtccC GCGCTGTACT GGAGGCTGAA 1441 GTcCAGgtaa gtttaaacag gatcttacta actaacatgc taacactgaa ttttcagATG 1501 TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT TATGGCAGGG TGAAACGCAG 1561 GTCGCCAGCG GCACCGCGCC TTTCGGCGGT GAAATTATCG ATGAGCGTGG TGGTTATGCC 1621 GATCGCGTCA CACTACGTCT GAACGTCGAA AACCCGAAAC TGTGGAGCGC CGAAATCCCG 1681 AATCTCTATC GTGCGGTGGT TGAACTGCAC ACCGCCGACG GCACGCTGAT TGAAGCAGAA 1741 GCCTGCGATG TCGGTTTCCG CGAGGTGCGG ATTGAAAATG GTCTGCTGCT GCTGAACGGC 1801 AAGCCGTTGC TGATTCGAGG CGTTAACCGT CACGAGCATC ATCCTCTGCA TGGTCAGGTC 1861 ATGGATGAGC AGACGATGGT GCAGGATgta agtttaaact attcgttact aactaacttt 1921 aaacatttaa attttcagAT CCTGCTGATG AAGCAGAACA ACTTTAACGC CGTGCGCTGT 1981 TCGCATTATC CGAACCATCC GCTGTGGTAC ACGCTGTGCG ACCGCTACGG CCTGTATGTG 2041 GTGGATGAAG CCAATATTGA AACCCACGGC ATGGTGCCAA TGAATCGTCT GACCGATGAT 2101 CCGCGCTGGC TACCGGCGAT GAGCGAACGC GTAACGCGAA TGGTGCAGCG CGATCGTAAT 2161 CACCCGAGTG TGATCATCTG GTCGCTGGGG AATGgtaagt ttaaacagtt gaatactaac 2221 taacggagat ctttgaaatt ttcagAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT 2281 CGCTGGATCA AATCTGTCGA TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC 2341 ACCACGGCCA CCGATATTAT TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC 2401 CCGGCTGTGC CGAAATGGTC CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG 2461 CTGATtCTTT GCGAGgtaag tttaaacaga actctactaa ctaacacatt agatcctaat 2521 tttcagTACG CtCACGCGAT GGGcAACAGT CTTGGCGGTT TCGCTAAATA CTGGCAGGCG 2581 TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG 2641 ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT TGGCGATACG 2701 CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC GCCGCATCCA 2761 GCgtaagttt aaacaataac ctactaacta acgtagataa tttaaatttt cagGCTGACG 2821 GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 2881 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG 2941 GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG 3001 TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA 3061 CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCaTGGCAG 3121 CAGTGGAGgt aagtttaaac aagatcctac taactaactc tacattgatg aattttcagA 3181 CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC ACGCCATCCC GCATCTGACC 3241 ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC GTTGGCAATT TAACCGCCAG 3301 TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC AACTGCTGAC GCCGCTGCGC 3361 GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG TAAGTGAAGC GACCCGCATT 3421 GACCCTAACG CCTGGGTCGg taagtttaaa caaagttgta ctaactaacg aagatcttga 3481 taattttcag AACGCTGGAA GGCGGCGGGC CATTACCAGG CCGAAGCAGC GTTGTTGCAG 3541 TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA CCGCTCACGC GTGGCAGCAT 3601 CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA TTGATGGTAG TGGTCAAATG 3661 GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC ATCCGGCGCG GATTGGCCTG 3721 AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC TCGGATTAGG GCCGCAAGAA 3781 AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT GGGATCTGCC ATTGTCAGAC 3841 ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt tcatttaaat tttcagTACC 3901 CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA CGCGCGAATT GAATTATGGC 3961 CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC GCTACAGTCA ACAGCAACTG 4021 ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG GCACATGGCT GAATATCGAC 4081 GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC CGTCAGTATC GGCGGAATTC 4141 CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG 4201 CTGTCATCAG Agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc 4261 agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG 4321 CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT 4381 TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA 4441 TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA 4501 ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT 4561 AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT 4621 TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT 4681 CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT 4741 ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC 4801 GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC 4861 GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT 4921 GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC 4981 ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 5041 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 5101 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 5161 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 5221 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC 5281 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 5341 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5401 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 5461 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 5521 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 5581 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 5641 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5701 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 5761 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 5821 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 5881 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 5941 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 6001 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 6061 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 6121 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 6181 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 6241 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 6301 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 6361 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6421 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6481 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6541 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6601 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 6661 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 6721 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 6781 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 6841 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC 6901 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 6961 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 7021 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 7081 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 7141 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 7201 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 7261 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 7321 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 7381 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 7441 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt 7501 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT 7561 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT // 1485 LOCUS ADDGENE_1485 7101 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_21 complete plasmid sequence KEYWORDS . SOURCE pPD95_21 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1485 at www.addgene.org FEATURES Location/Qualifiers source 1..7101 /organism="Addgene plasmid pPD95_21" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..3682 /label="lacZ" /gene="lacZ" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKS VDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLIL CEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFG DTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNEL LHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWS EAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQ MWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQC TADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIG LNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGT RELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSW SPSVSAEFQLSAGRYHYQLVWCQK" gene 635..3679 /label="lacZ" /gene="lacZ" misc_feature 3703..3753 /label="SynIVS.L" promoter 4881..4909 /label="Amp_promoter" CDS 4951..5811 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 4951..5811 /label="Ampicillin" /gene="Ampicillin" rep_origin 5966..6585 /label="pBR322_origin" misc_feature 6801..6827 /label="lacI.C" promoter 6894..6923 /label="lac_promoter" misc_feature 6958..6976 /label="M13r_primer" misc_feature 6990..7040 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 781 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 841 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 901 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 961 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 1021 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 1081 CTGGGTCGGT TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT 1141 ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT 1201 GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA 1261 ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG 1321 CGCTGTACTG GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 1381 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 1441 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 1501 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 1561 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 1621 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 1681 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATATCCT 1741 GCTGATGAAG CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT 1801 GTGGTACACG CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC 1861 CCACGGCATG GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG 1921 CGAACGCGTA ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC 1981 GCTGGGGAAT GAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT GGATCAAATC 2041 TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA CGGCCACCGA 2101 TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG CTGTGCCGAA 2161 ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA TCCTTTGCGA 2221 ATACGCCCAC GCGATGGGTA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG 2281 TCAGTATCCC CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA 2341 ATATGATGAA AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA 2401 CGATCGCCAG TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCGCT 2461 GACGGAAGCA AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA 2521 AGTGACCAGC GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC 2581 GCTGGATGGT AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA 2641 ACAGTTGATT GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC 2701 AGTACGCGTA GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCCTG 2761 GCAGCAGTGG CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT CCCACGCCAT 2821 CCCGCATCTG ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA AGCGTTGGCA 2881 ATTTAACCGC CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA AACAACTGCT 2941 GACGCCGCTG CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG GCGTAAGTGA 3001 AGCGACCCGC ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG GCCATTACCA 3061 GGCCGAAGCA GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC 3121 GACCGCTCAC GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG 3181 GATTGATGGT AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC 3241 GCATCCGGCG CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG 3301 GCTCGGATTA GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG 3361 CTGGGATCTG CCATTGTCAG ACATGTATAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT 3421 GCGCTGCGGG ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT 3481 CAACATCAGC CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA 3541 CGCGGAAGAA GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA 3601 CTCCTGGAGC CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 3661 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 3721 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 3781 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 3841 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 3901 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 3961 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 4021 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 4081 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 4141 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 4201 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 4261 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 4321 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 4381 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 4441 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 4501 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 4561 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 4621 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 4681 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 4741 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 4801 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 4861 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 4921 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 4981 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 5041 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 5101 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 5161 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 5221 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 5281 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 5341 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 5401 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 5461 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 5521 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 5581 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 5641 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 5701 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 5761 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 5821 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 5881 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 5941 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 6001 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 6061 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 6121 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 6181 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 6241 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 6301 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 6361 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 6421 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 6481 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 6541 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 6601 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 6661 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 6721 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 6781 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 6841 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 6901 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 6961 AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta ctaactaact 7021 attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC GATGGATACG 7081 CTAACAACTT GGAAATGAAA T // 1486 LOCUS ADDGENE_1486 7406 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_25 complete plasmid sequence KEYWORDS . SOURCE pPD95_25 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1486 at www.addgene.org FEATURES Location/Qualifiers source 1..7406 /organism="Addgene plasmid pPD95_25" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..1180 /label="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLN LT" gene 635..3984 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" CDS 1219..3987 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNG KPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAV RCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQ RDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHDALYRWIKSVDPSRPVQY EGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGN SLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFC MNGLVFADRTPHPA*V*TITY*LT*II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFN RQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN* RRS**FSERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRI DGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFD RWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQF NISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYH YQLVWCQK" misc_feature 1786..1836 /label="SynIVS.E" misc_feature 2093..2143 /label="SynIVS.F" misc_feature 2610..2660 /label="SynIVS.H" misc_feature 3236..3286 /label="SynIVS.J" misc_feature 3642..3692 /label="SynIVS.K" misc_feature 4008..4058 /label="SynIVS.L" promoter 5186..5214 /label="Amp_promoter" gene 5256..6116 /label="Ampicillin" /gene="Ampicillin" CDS 5256..6116 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6271..6890 /label="pBR322_origin" misc_feature 7106..7132 /label="lacI.C" promoter 7199..7228 /label="lac_promoter" misc_feature 7263..7281 /label="M13r_primer" misc_feature 7295..7345 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 781 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 841 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 901 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 961 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 1021 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 1081 CTGGGTCGGT TACGGCCAGg taagtttaat taagttgata CTAACTAACA AGATCTGATT 1141 AATTTTCagG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT ACGCGCCGGA 1201 GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT GGAAGATCAG 1261 GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA ACCGACTACA 1321 CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG CGCTGTACTG 1381 GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC AGTTTCTTTA 1441 TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA AATTATCGAT 1501 GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA CCCGAAACTG 1561 TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC CGCCGACGGC 1621 ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT TGAAAATGGT 1681 CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA CGAGCATCAT 1741 CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag tttaaactat 1801 tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA GCAGAACAAC 1861 TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC GCTGTGCGAC 1921 CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT GGTGCCAATG 1981 AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT AACGCGAATG 2041 GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA TGgtaagttt 2101 aaacagttga atactaacta acggagatct ttgaaatttt cagAATCAGG CCACGGCGCT 2161 AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC CTTCCCGCCC GGTGCAGTAT 2221 GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT GCCCGATGTA CGCGCGCGTG 2281 GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA TCAAAAAATG GCTTTCGCTA 2341 CCTGGAGAGA CGCGCCCGCT GATCCTTTGC GAATACGCCC ACGCGATGGG TAACAGTCTT 2401 GGCGGTTTCG CTAAATACTG GCAGGCGTTT CGTCAGTATC CCCGTTTACA GGGCGGCTTC 2461 GTCTGGGACT GGGTGGATCA GTCGCTGATT AAATATGATG AAAACGGCAA CCCGTGGTCG 2521 GCTTACGGCG GTGATTTTGG CGATACGCCG AACGATCGCC AGTTCTGTAT GAACGGTCTG 2581 GTCTTTGCCG ACCGCACGCC GCATCCAGCg taagtttaaa caataaccta ctaactaacg 2641 tagataattt aaattttcag GCTGACGGAA GCAAAACACC AGCAGCAGTT TTTCCAGTTC 2701 CGTTTATCCG GGCAAACCAT CGAAGTGACC AGCGAATACC TGTTCCGTCA TAGCGATAAC 2761 GAGCTCCTGC ACTGGATGGT GGCGCTGGAT GGTAAGCCGC TGGCAAGCGG TGAAGTGCCT 2821 CTGGATGTCG CTCCACAAGG TAAACAGTTG ATTGAACTGC CTGAACTACC GCAGCCGGAG 2881 AGCGCCGGGC AACTCTGGCT CACAGTACGC GTAGTGCAAC CGAACGCGAC CGCATGGTCA 2941 GAAGCCGGGC ACATCAGCGC CTGGCAGCAG TGGCGTCTGG CGGAAAACCT CAGTGTGACG 3001 CTCCCCGCCG CGTCCCACGC CATCCCGCAT CTGACCACCA GCGAAATGGA TTTTTGCATC 3061 GAGCTGGGTA ATAAGCGTTG GCAATTTAAC CGCCAGTCAG GCTTTCTTTC ACAGATGTGG 3121 ATTGGCGATA AAAAACAACT GCTGACGCCG CTGCGCGATC AGTTCACCCG TGCACCGCTG 3181 GATAACGACA TTGGCGTAAG TGAAGCGACC CGCATTGACC CTAACGCCTG GGTCGgtaag 3241 tttaaacaaa gttgtactaa ctaacgaaga tcttgataat tttcagAACG CTGGAAGGCG 3301 GCGGGCCATT ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA CGGCAGATAC ACTTGCTGAT 3361 GCGGTGCTGA TTACGACCGC TCACGCGTGG CAGCATCAGG GGAAAACCTT ATTTATCAGC 3421 CGGAAAACCT ACCGGATTGA TGGTAGTGGT CAAATGGCGA TTACCGTTGA TGTTGAAGTG 3481 GCGAGCGATA CACCGCATCC GGCGCGGATT GGCCTGAACT GCCAGCTGGC GCAGGTAGCA 3541 GAGCGGGTAA ACTGGCTCGG ATTAGGGCCG CAAGAAAACT ATCCCGACCG CCTTACTGCC 3601 GCCTGTTTTG ACCGCTGGGA TCTGCCATTG TCAGACATGT Agtaagttta aacttgatag 3661 tactaactaa catgtttcat ttaaattttc agTACCCCGT ACGTCTTCCC GAGCGAAAAC 3721 GGTCTGCGCT GCGGGACGCG CGAATTGAAT TATGGCCCAC ACCAGTGGCG CGGCGACTTC 3781 CAGTTCAACA TCAGCCGCTA CAGTCAACAG CAACTGATGG AAACCAGCCA TCGCCATCTG 3841 CTGCACGCGG AAGAAGGCAC ATGGCTGAAT ATCGACGGTT TCCATATGGG GATTGGTGGC 3901 GACGACTCCT GGAGCCCGTC AGTATCGGCG GAATTCCAAC TGAGCGCCGG TCGCTACCAT 3961 TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGAgta agtttaaact 4021 gagttctact aactaacgag taatatttaa attttcagCA TCTCGCGCCC GTGCCTCTGA 4081 CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT GCTCCCACCC 4141 CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG CTTCTTTTAA 4201 GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT AATAAAAAGT 4261 CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA TCTACACAAT 4321 GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA CATCATAGAA 4381 AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC GCAATTTTTA 4441 TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA AAAAGTTTTG 4501 GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT CCTGCTTTTG 4561 CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT TCTTGCTAAA 4621 ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG GTTTGAGGCT 4681 CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT GGGGTTTTTG 4741 CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA CTAGTCGGCC 4801 GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA 4861 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA 4921 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA 4981 GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA 5041 ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA 5101 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 5161 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 5221 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 5281 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 5341 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 5401 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 5461 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 5521 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 5581 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 5641 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 5701 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 5761 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 5821 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 5881 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 5941 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 6001 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 6061 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT 6121 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA 6181 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT 6241 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 6301 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 6361 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 6421 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 6481 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 6541 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 6601 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 6661 GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 6721 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 6781 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 6841 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 6901 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT 6961 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA 7021 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC 7081 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG 7141 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT 7201 TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA 7261 CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt ttaaacatga tcttactaac 7321 taactattct catttaaatt ttcagAGCTT AAAAATGGCT GAAATCACTC ACAACGATGG 7381 ATACGCTAAC AACTTGGAAA TGAAAT // 1487 LOCUS ADDGENE_1487 7254 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_27 complete plasmid sequence KEYWORDS . SOURCE pPD95_27 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1487 at www.addgene.org FEATURES Location/Qualifiers source 1..7254 /organism="Addgene plasmid pPD95_27" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..3835 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPE SWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNV DESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVM VLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQ MCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSA EIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHP LHGQVMDEQTMVQDVSLNYSLLTNFKHLNFQILLMKQNNFNAVRCSHYPNHPLWYTLC DRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGN ESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKW SIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT*II*IFRL TEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQ GKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAA SHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDN DIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTL FISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYP DRLTAACFDRWDLPLSDM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGP HQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAE FQLSAGRYHYQLVWCQK" gene 635..3832 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1736..1786 /label="SynIVS.E" misc_feature 2509..2559 /label="SynIVS.H" misc_feature 3490..3540 /label="SynIVS.K" misc_feature 3856..3906 /label="SynIVS.L" promoter 5034..5062 /label="Amp_promoter" CDS 5104..5964 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5104..5964 /label="Ampicillin" /gene="Ampicillin" rep_origin 6119..6738 /label="pBR322_origin" misc_feature 6954..6980 /label="lacI.C" promoter 7047..7076 /label="lac_promoter" misc_feature 7111..7129 /label="M13r_primer" misc_feature 7143..7193 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCCCGGGGAT TGGCCAAAGG 61 ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag GAGGACCCTT 121 GCTTGGCTAG CAAAAATGCA TAAGGTTTTG CTGGCACTGT TCTTTATCTT TCTGGCACCA 181 GCATCCGCAC TGGCAGTCTC CGAACCGGCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGGC ATTGATTGGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA CTGAGCCGCG ATATTGCCCA 601 GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT TTACAACGTC GTGACTGGGA 661 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 721 TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA 781 ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA 841 TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC 901 GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA 961 GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG 1021 CCAGACGCGA ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG 1081 CTGGGTCGGT TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT 1141 ACGCGCCGGA GAAAACCGCC TCGCGGTGAT GGTGCTGCGC TGGAGTGACG GCAGTTATCT 1201 GGAAGATCAG GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA 1261 ACCGACTACA CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG 1321 CGCTGTACTG GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC 1381 AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA 1441 AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA 1501 CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC 1561 CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT 1621 TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA 1681 CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATgtaag 1741 tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC TGCTGATGAA 1801 GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC TGTGGTACAC 1861 GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA CCCACGGCAT 1921 GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA GCGAACGCGT 1981 AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT CGCTGGGGAA 2041 TGAATCAGGC CACGGCGCTA ATCACGACGC GCTGTATCGC TGGATCAAAT CTGTCGATCC 2101 TTCCCGCCCG GTGCAGTATG AAGGCGGCGG AGCCGACACC ACGGCCACCG ATATTATTTG 2161 CCCGATGTAC GCGCGCGTGG ATGAAGACCA GCCCTTCCCG GCTGTGCCGA AATGGTCCAT 2221 CAAAAAATGG CTTTCGCTAC CTGGAGAGAC GCGCCCGCTG ATCCTTTGCG AATACGCCCA 2281 CGCGATGGGT AACAGTCTTG GCGGTTTCGC TAAATACTGG CAGGCGTTTC GTCAGTATCC 2341 CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG TCGCTGATTA AATATGATGA 2401 AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC GATACGCCGA ACGATCGCCA 2461 GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG CATCCAGCgt aagtttaaac 2521 aataacctac taactaacgt agataattta aattttcagG CTGACGGAAG CAAAACACCA 2581 GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC GAAGTGACCA GCGAATACCT 2641 GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG GCGCTGGATG GTAAGCCGCT 2701 GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT AAACAGTTGA TTGAACTGCC 2761 TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC ACAGTACGCG TAGTGCAACC 2821 GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCC TGGCAGCAGT GGCGTCTGGC 2881 GGAAAACCTC AGTGTGACGC TCCCCGCCGC GTCCCACGCC ATCCCGCATC TGACCACCAG 2941 CGAAATGGAT TTTTGCATCG AGCTGGGTAA TAAGCGTTGG CAATTTAACC GCCAGTCAGG 3001 CTTTCTTTCA CAGATGTGGA TTGGCGATAA AAAACAACTG CTGACGCCGC TGCGCGATCA 3061 GTTCACCCGT GCACCGCTGG ATAACGACAT TGGCGTAAGT GAAGCGACCC GCATTGACCC 3121 TAACGCCTGG GTCGAACGCT GGAAGGCGGC GGGCCATTAC CAGGCCGAAG CAGCGTTGTT 3181 GCAGTGCACG GCAGATACAC TTGCTGATGC GGTGCTGATT ACGACCGCTC ACGCGTGGCA 3241 GCATCAGGGG AAAACCTTAT TTATCAGCCG GAAAACCTAC CGGATTGATG GTAGTGGTCA 3301 AATGGCGATT ACCGTTGATG TTGAAGTGGC GAGCGATACA CCGCATCCGG CGCGGATTGG 3361 CCTGAACTGC CAGCTGGCGC AGGTAGCAGA GCGGGTAAAC TGGCTCGGAT TAGGGCCGCA 3421 AGAAAACTAT CCCGACCGCC TTACTGCCGC CTGTTTTGAC CGCTGGGATC TGCCATTGTC 3481 AGACATGTAg taagtttaaa cttgatagta ctaactaaca tgtttcattt aaattttcag 3541 TACCCCGTAC GTCTTCCCGA GCGAAAACGG TCTGCGCTGC GGGACGCGCG AATTGAATTA 3601 TGGCCCACAC CAGTGGCGCG GCGACTTCCA GTTCAACATC AGCCGCTACA GTCAACAGCA 3661 ACTGATGGAA ACCAGCCATC GCCATCTGCT GCACGCGGAA GAAGGCACAT GGCTGAATAT 3721 CGACGGTTTC CATATGGGGA TTGGTGGCGA CGACTCCTGG AGCCCGTCAG TATCGGCGGA 3781 ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG 3841 GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa ctaacgagta atatttaaat 3901 tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA 3961 CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT 4021 AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA 4081 AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT 4141 AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC 4201 TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT 4261 ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC 4321 AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA 4381 GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA 4441 TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA 4501 GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA 4561 AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC 4621 AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG 4681 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 4741 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 4801 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 4861 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG 4921 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 4981 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 5041 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 5101 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 5161 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 5221 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 5281 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 5341 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 5401 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 5461 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 5521 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 5581 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 5641 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 5701 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 5761 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 5821 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 5881 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 5941 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 6001 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 6061 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 6121 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 6181 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 6241 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 6301 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 6361 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 6421 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 6481 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 6541 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 6601 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 6661 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 6721 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 6781 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 6841 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 6901 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 6961 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 7021 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 7081 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG 7141 CTgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagAGCTTAA 7201 AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA CTTGGAAATG AAAT // 1488 LOCUS ADDGENE_1488 7228 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_57 complete plasmid sequence KEYWORDS . SOURCE pPD95_57 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1488 at www.addgene.org FEATURES Location/Qualifiers source 1..7228 /organism="Addgene plasmid pPD95_57" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" misc_feature 136..162 /label="SV40.NLS" CDS 136..849 /label="Orf0" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 253..3806 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 387..437 /label="SynIVS.B" CDS 888..3809 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1065..1115 /label="SynIVS.D" misc_feature 1506..1556 /label="SynIVS.E" misc_feature 1813..1863 /label="SynIVS.F" misc_feature 2094..2144 /label="SynIVS.G" misc_feature 2381..2431 /label="SynIVS.H" misc_feature 2747..2797 /label="SynIVS.I" misc_feature 3058..3108 /label="SynIVS.J" misc_feature 3464..3514 /label="SynIVS.K" misc_feature 3830..3880 /label="SynIVS.L" promoter 5008..5036 /label="Amp_promoter" gene 5078..5938 /label="Ampicillin" /gene="Ampicillin" CDS 5078..5938 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6093..6712 /label="pBR322_origin" misc_feature 6928..6954 /label="lacI.C" promoter 7021..7050 /label="lac_promoter" misc_feature 7085..7103 /label="M13r_primer" misc_feature 7117..7167 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT GGGTGAAGAC 181 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 241 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 301 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 361 GATCGCCCTT CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt 421 tctgacataa ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA 481 GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC 541 TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT 601 ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT 661 AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC 721 TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt 781 aagttgataC TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT 841 TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT 901 GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG 961 ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT 1021 TTAATGATGA TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga 1081 tcttactaac taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC 1141 TACGGGTAAC AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT 1201 TCGGCGGTGA AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA 1261 ACGTCGAAAA CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG 1321 AACTGCACAC CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG 1381 AGGTGCGGAT TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG 1441 TTAACCGTCA CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC 1501 AGGATgtaag tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC 1561 TGCTGATGAA GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC 1621 TGTGGTACAC GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA 1681 CCCACGGCAT GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA 1741 GCGAACGCGT AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT 1801 CGCTGGGGAA TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt 1861 cagAATCAGG CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC 1921 CTTCCCGCCC GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT 1981 GCCCGATGTA CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA 2041 TCAAAAAATG GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt 2101 taaacagaac tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG 2161 GcAACAGTCT TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC 2221 AGGGCGGCTT CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA 2281 ACCCGTGGTC GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA 2341 TGAACGGTCT GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct 2401 actaactaac gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT 2461 TTTTCCAGTT CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC 2521 ATAGCGATAA CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG 2581 GTGAAGTGCC TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC 2641 CGCAGCCGGA GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA 2701 CCGCATGGTC AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa 2761 gatcctacta actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA 2821 CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA 2881 TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT 2941 GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC 3001 TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta 3061 agtttaaaca aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG 3121 CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG 3181 ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA 3241 GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG 3301 TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG 3361 CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG 3421 CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat 3481 agtactaact aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA 3541 ACGGTCTGCG CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT 3601 TCCAGTTCAA CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC 3661 TGCTGCACGC GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG 3721 GCGACGACTC CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC 3781 ATTACCAACT TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa 3841 ctgagttcta ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT 3901 GACTTCTAAG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC 3961 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT 4021 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA 4081 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA 4141 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG 4201 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT 4261 TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT 4321 TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT 4381 TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA 4441 AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG 4501 CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT 4561 TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG 4621 CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 4681 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 4741 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 4801 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 4861 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 4921 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 4981 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 5041 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 5101 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 5161 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 5221 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 5281 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 5341 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 5401 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 5461 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 5521 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 5581 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 5641 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 5701 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 5761 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 5821 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 5881 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 5941 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 6001 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 6061 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 6121 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 6181 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 6241 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 6301 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 6361 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 6421 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 6481 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 6541 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 6601 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 6661 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 6721 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 6781 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 6841 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 6901 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 6961 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 7021 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 7081 CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTgtaa gtttaaacat gatcttacta 7141 actaactatt ctcatttaaa ttttcagAGC TTAAAAATGG CTGAAATCAC TCACAACGAT 7201 GGATACGCTA ACAACTTGGA AATGAAAT // 1489 LOCUS ADDGENE_1489 7390 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_62 complete plasmid sequence KEYWORDS . SOURCE pPD95_62 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1489 at www.addgene.org FEATURES Location/Qualifiers source 1..7390 /organism="Addgene plasmid pPD95_62" /mol_type="other DNA" misc_feature 32..56 /label="myo2.delta-90" misc_feature 123..146 /label="myo2.5'" misc_feature 220..261 /label="SynIVS.A" CDS 298..1011 /label="Orf0" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" misc_feature 298..324 /label="SV40.NLS" gene 415..3968 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 549..599 /label="SynIVS.B" CDS 1050..3971 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1227..1277 /label="SynIVS.D" misc_feature 1668..1718 /label="SynIVS.E" misc_feature 1975..2025 /label="SynIVS.F" misc_feature 2256..2306 /label="SynIVS.G" misc_feature 2543..2593 /label="SynIVS.H" misc_feature 2909..2959 /label="SynIVS.I" misc_feature 3220..3270 /label="SynIVS.J" misc_feature 3626..3676 /label="SynIVS.K" misc_feature 3992..4042 /label="SynIVS.L" promoter 5170..5198 /label="Amp_promoter" gene 5240..6100 /label="Ampicillin" /gene="Ampicillin" CDS 5240..6100 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6255..6874 /label="pBR322_origin" misc_feature 7090..7116 /label="lacI.C" promoter 7183..7212 /label="lac_promoter" misc_feature 7247..7265 /label="M13r_primer" misc_feature 7279..7329 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGACACT TTTCAGCTAC CTAGATACAT 61 GGATATCCCC GCCTCCCAAT CCACCCACCC AGGGAAAAAG AAGGGCTCGC CGAAAATCAA 121 AGTTATCTCC AGGCTCGCGC ATCCCACCGA GCGGTTGACT TCTCTCCACC ACTTTTCATT 181 TTAACCCTCG GGGTACGGGA TTGGCCAAAG GACCCAAAGG TATGTTTCGA ATGATACTAA 241 CATAACATAG AACATTTTCA GGAGGACCct tCTTGGAGGG TACCGAGCTC AGAAAAAATG 301 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GTGGGTGAAG ACCAGAAACA GCACCTCGAA 361 CTGAGCCGCG ATATTGCCCA GCGTTTCAAC GCGCTGTATG GCGAGATCGA TCCCGTCGTT 421 TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT 481 CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG 541 TTGCGaAGgt aagtttaaac agatccatac taactaactt gttctgacat aattttcagC 601 tTGAATGGCG AATGGCGCTT TGCCTGGTTT CCGGCACCAG AAGCGGTGCC GGAAAGCTGG 661 CTGGAGTGCG ATCTTCCTGA GGCCGATACT GTCGTCGTCC CCTCAAACTG GCAGATGCAC 721 GGTTACGATG CGCCCATCTA CACCAACGTG ACCTATCCCA TTACGGTCAA TCCGCCGTTT 781 GTTCCCACGG AGAATCCGAC GGGTTGTTAC TCGCTCACAT TTAATGTTGA TGAAAGCTGG 841 CTACAGGAAG GCCAGACGCG AATTATTTTT GATGGCGTTA ACTCGGCGTT TCATCTGTGG 901 TGCAACGGGC GCTGGGTCGG TTACGGCCAG gtaagtttaa ttaagttgat aCTAACTAAC 961 AAGATCTGAT TAATTTTCag GACAGTCGTT TGCCGTCTGA ATTTGACCTG AGCGCATTTT 1021 TACGCGCCGG AGAAAACCGC CTCGCGGTGA TGGTGCTGCG CTGGAGTGAC GGCAGTTATC 1081 TGGAAGATCA GGATATGTGG CGGATGAGCG GCATTTTCCG TGACGTCTCG TTGCTGCATA 1141 AACCGACTAC ACAAATCAGC GATTTCCATG TTGCCACTCG CTTTAATGAT GATTTCtccC 1201 GCGCTGTACT GGAGGCTGAA GTcCAGgtaa gtttaaacag gatcttacta actaacatgc 1261 taacactgaa ttttcagATG TGCGGCGAGT TGCGTGACTA CCTACGGGTA ACAGTTTCTT 1321 TATGGCAGGG TGAAACGCAG GTCGCCAGCG GCACCGCGCC TTTCGGCGGT GAAATTATCG 1381 ATGAGCGTGG TGGTTATGCC GATCGCGTCA CACTACGTCT GAACGTCGAA AACCCGAAAC 1441 TGTGGAGCGC CGAAATCCCG AATCTCTATC GTGCGGTGGT TGAACTGCAC ACCGCCGACG 1501 GCACGCTGAT TGAAGCAGAA GCCTGCGATG TCGGTTTCCG CGAGGTGCGG ATTGAAAATG 1561 GTCTGCTGCT GCTGAACGGC AAGCCGTTGC TGATTCGAGG CGTTAACCGT CACGAGCATC 1621 ATCCTCTGCA TGGTCAGGTC ATGGATGAGC AGACGATGGT GCAGGATgta agtttaaact 1681 attcgttact aactaacttt aaacatttaa attttcagAT CCTGCTGATG AAGCAGAACA 1741 ACTTTAACGC CGTGCGCTGT TCGCATTATC CGAACCATCC GCTGTGGTAC ACGCTGTGCG 1801 ACCGCTACGG CCTGTATGTG GTGGATGAAG CCAATATTGA AACCCACGGC ATGGTGCCAA 1861 TGAATCGTCT GACCGATGAT CCGCGCTGGC TACCGGCGAT GAGCGAACGC GTAACGCGAA 1921 TGGTGCAGCG CGATCGTAAT CACCCGAGTG TGATCATCTG GTCGCTGGGG AATGgtaagt 1981 ttaaacagtt gaatactaac taacggagat ctttgaaatt ttcagAATCA GGCCACGGCG 2041 CTAATCACGA CGCGCTGTAT CGCTGGATCA AATCTGTCGA TCCTTCCCGC CCGGTGCAGT 2101 ATGAAGGCGG CGGAGCCGAC ACCACGGCCA CCGATATTAT TTGCCCGATG TACGCGCGCG 2161 TGGATGAAGA CCAGCCCTTC CCGGCTGTGC CGAAATGGTC CATCAAAAAA TGGCTTTCGC 2221 TACCTGGAGA GACGCGCCCG CTGATtCTTT GCGAGgtaag tttaaacaga actctactaa 2281 ctaacacatt agatcctaat tttcagTACG CtCACGCGAT GGGcAACAGT CTTGGCGGTT 2341 TCGCTAAATA CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG 2401 ACTGGGTGGA TCAGTCGCTG ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG 2461 GCGGTGATTT TGGCGATACG CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG 2521 CCGACCGCAC GCCGCATCCA GCgtaagttt aaacaataac ctactaacta acgtagataa 2581 tttaaatttt cagGCTGACG GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT 2641 CCGGGCAAAC CATCGAAGTG ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC 2701 TGCACTGGAT GGTGGCGCTG GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG 2761 TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG 2821 GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG 2881 GGCACATCAG CGCaTGGCAG CAGTGGAGgt aagtttaaac aagatcctac taactaactc 2941 tacattgatg aattttcagA CTGGCGGAAA ACCTCAGTGT GACGCTCCCC GCCGCGTCCC 3001 ACGCCATCCC GCATCTGACC ACCAGCGAAA TGGATTTTTG CATCGAGCTG GGTAATAAGC 3061 GTTGGCAATT TAACCGCCAG TCAGGCTTTC TTTCACAGAT GTGGATTGGC GATAAAAAAC 3121 AACTGCTGAC GCCGCTGCGC GATCAGTTCA CCCGTGCACC GCTGGATAAC GACATTGGCG 3181 TAAGTGAAGC GACCCGCATT GACCCTAACG CCTGGGTCGg taagtttaaa caaagttgta 3241 ctaactaacg aagatcttga taattttcag AACGCTGGAA GGCGGCGGGC CATTACCAGG 3301 CCGAAGCAGC GTTGTTGCAG TGCACGGCAG ATACACTTGC TGATGCGGTG CTGATTACGA 3361 CCGCTCACGC GTGGCAGCAT CAGGGGAAAA CCTTATTTAT CAGCCGGAAA ACCTACCGGA 3421 TTGATGGTAG TGGTCAAATG GCGATTACCG TTGATGTTGA AGTGGCGAGC GATACACCGC 3481 ATCCGGCGCG GATTGGCCTG AACTGCCAGC TGGCGCAGGT AGCAGAGCGG GTAAACTGGC 3541 TCGGATTAGG GCCGCAAGAA AACTATCCCG ACCGCCTTAC TGCCGCCTGT TTTGACCGCT 3601 GGGATCTGCC ATTGTCAGAC ATGTAgtaag tttaaacttg atagtactaa ctaacatgtt 3661 tcatttaaat tttcagTACC CCGTACGTCT TCCCGAGCGA AAACGGTCTG CGCTGCGGGA 3721 CGCGCGAATT GAATTATGGC CCACACCAGT GGCGCGGCGA CTTCCAGTTC AACATCAGCC 3781 GCTACAGTCA ACAGCAACTG ATGGAAACCA GCCATCGCCA TCTGCTGCAC GCGGAAGAAG 3841 GCACATGGCT GAATATCGAC GGTTTCCATA TGGGGATTGG TGGCGACGAC TCCTGGAGCC 3901 CGTCAGTATC GGCGGAATTC CAACTGAGCG CCGGTCGCTA CCATTACCAA CTTGTCTGGT 3961 GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta aactgagttc tactaactaa 4021 cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT CTGACTTCTA AGTCCAATTA 4081 CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC ACCCCCTATT TTTGTTATTA 4141 TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT TTAAGTCACC TCTAACAATG 4201 AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA AAGTCGAAAA AAATTGTGCT 4261 CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA CAATGTTCTG TGTACACTTC 4321 TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT AGAAAAAACC GCACACAAAA 4381 TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT TTTATTTCTT CGCACGTCTG 4441 GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT TTTGGAGTAT TTTTGGAATT 4501 TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT TTTGCTTTTT GGGGTTTCCC 4561 CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG 4621 AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA 4681 GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT 4741 ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT 4801 CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC 4861 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 4921 GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG 4981 CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG 5041 CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 5101 TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 5161 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 5221 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 5281 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC 5341 TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 5401 CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT 5461 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 5521 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 5581 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA 5641 CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 5701 GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 5761 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 5821 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 5881 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG 5941 AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 6001 CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 6061 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC 6121 ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 6181 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 6241 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 6301 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 6361 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 6421 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 6481 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 6541 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 6601 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 6661 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 6721 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 6781 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 6841 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 6901 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 6961 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 7021 AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC 7081 GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 7141 CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC 7201 GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA 7261 CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac taactaacta ttctcattta 7321 aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG 7381 GAAATGAAAT // 1490 LOCUS ADDGENE_1490 4532 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_67 complete plasmid sequence KEYWORDS . SOURCE pPD95_67 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1490 at www.addgene.org FEATURES Location/Qualifiers source 1..4532 /organism="Addgene plasmid pPD95_67" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 137..163 /label="SV40.NLS" CDS 137..1048 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 179..1039 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 350..400 /label="SynIVS.R" misc_feature 422..441 /label="gfp.S65C" misc_feature 555..605 /label="SynIVS.S" misc_feature 770..820 /label="SynIVS.T" misc_feature 1134..1184 /label="SynIVS.L" promoter 2312..2340 /label="Amp_promoter" CDS 2382..3242 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2382..3242 /label="Ampicillin" /gene="Ampicillin" rep_origin 3397..4016 /label="pBR322_origin" misc_feature 4232..4258 /label="lacI.C" promoter 4325..4354 /label="lac_promoter" misc_feature 4389..4407 /label="M13r_primer" misc_feature 4421..4471 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 181 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 241 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 301 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 361 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 421 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 481 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 541 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 601 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 661 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 721 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 781 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 841 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 901 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 961 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1021 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1081 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1141 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1201 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1261 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1321 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1381 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1441 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1501 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1561 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1621 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1681 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1741 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 1801 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 1861 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 1921 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 1981 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2041 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2101 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2161 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2221 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2281 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2341 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2401 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2461 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2521 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2581 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2641 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2701 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 2761 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 2821 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 2881 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 2941 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3001 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3061 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3121 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3181 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3241 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3301 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3361 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3421 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3481 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3541 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3601 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3661 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3721 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 3781 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 3841 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 3901 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 3961 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4021 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4081 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4141 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4201 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4261 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4321 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4381 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT gtaagtttaa acatgatctt 4441 actaactaac tattctcatt taaattttca gAGCTTAAAA ATGGCTGAAA TCACTCACAA 4501 CGATGGATAC GCTAACAACT TGGAAATGAA AT // 1491 LOCUS ADDGENE_1491 4531 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_69 complete plasmid sequence KEYWORDS . SOURCE pPD95_69 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1491 at www.addgene.org FEATURES Location/Qualifiers source 1..4531 /organism="Addgene plasmid pPD95_69" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" misc_feature 136..162 /label="SV40.NLS" CDS 136..1047 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 178..1038 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 349..399 /label="SynIVS.R" misc_feature 421..440 /label="gfp.S65C" misc_feature 554..604 /label="SynIVS.S" misc_feature 769..819 /label="SynIVS.T" misc_feature 1133..1183 /label="SynIVS.L" promoter 2311..2339 /label="Amp_promoter" gene 2381..3241 /label="Ampicillin" /gene="Ampicillin" CDS 2381..3241 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3396..4015 /label="pBR322_origin" misc_feature 4231..4257 /label="lacI.C" promoter 4324..4353 /label="lac_promoter" misc_feature 4388..4406 /label="M13r_primer" misc_feature 4420..4470 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGAGCTCAG AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT AGAAAAAATG 181 AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT 241 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA 301 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac 361 atatatatac taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC 421 TgTTATGGTG TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC 481 AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG 541 AACTACAAGA CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt 601 tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT 661 ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA 721 CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac 781 atgattttac taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC 841 ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT 901 GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT 961 CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA 1021 CATGGCATGG ATGAACTATA CAAATAGCAT TCGTAGAATT CCAACTGAGC GCCGGTCGCT 1081 ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt 1141 aaactgagtt ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC 1201 TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC 1261 CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT 1321 TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA 1381 AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC 1441 ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA 1501 TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT 1561 TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG 1621 TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC 1681 TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG 1741 CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG 1801 AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT 1861 TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT 1921 CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC 1981 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC 2041 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA 2101 GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 2161 AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA 2221 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG 2281 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA 2341 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG 2401 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 2461 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT 2521 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT 2581 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA 2641 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC 2701 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 2761 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC 2821 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT 2881 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC 2941 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA 3001 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 3061 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT 3121 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC 3181 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA 3241 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT 3301 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 3361 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3421 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3481 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3541 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3601 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3661 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3721 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3781 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 3841 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 3901 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 3961 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4021 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC 4081 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG 4141 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC 4201 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 4261 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA 4321 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT 4381 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta 4441 ctaactaact attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC 4501 GATGGATACG CTAACAACTT GGAAATGAAA T // 1492 LOCUS ADDGENE_1492 4536 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_70 complete plasmid sequence KEYWORDS . SOURCE pPD95_70 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1492 at www.addgene.org FEATURES Location/Qualifiers source 1..4536 /organism="Addgene plasmid pPD95_70" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" CDS 141..1052 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 141..167 /label="SV40.NLS" gene 183..1043 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 354..404 /label="SynIVS.R" misc_feature 426..445 /label="gfp.S65C" misc_feature 559..609 /label="SynIVS.S" misc_feature 774..824 /label="SynIVS.T" misc_feature 1138..1188 /label="SynIVS.L" promoter 2316..2344 /label="Amp_promoter" CDS 2386..3246 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2386..3246 /label="Ampicillin" /gene="Ampicillin" rep_origin 3401..4020 /label="pBR322_origin" misc_feature 4236..4262 /label="lacI.C" promoter 4329..4358 /label="lac_promoter" misc_feature 4393..4411 /label="M13r_primer" misc_feature 4425..4475 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA 181 AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG 241 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 301 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt 361 taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA 421 CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT 481 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 541 ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta 601 aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA 661 AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA 721 ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt 781 taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC 841 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 901 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA 961 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 1021 TTACACATGG CATGGATGAA CTATACAAAT AGCATTCGTA GAATTCCAAC TGAGCGCCGG 1081 TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGAgta 1141 agtttaaact gagttctact aactaacgag taatatttaa attttcagCA TCTCGCGCCC 1201 GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT 1261 GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG 1321 CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT 1381 AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA 1441 TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA 1501 CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC 1561 GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA 1621 AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT 1681 CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT 1741 TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG 1801 GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT 1861 GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA 1921 CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT 1981 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC 2041 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG 2101 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG 2161 TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG 2221 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 2281 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 2341 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 2401 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 2461 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 2521 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 2581 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 2641 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 2701 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 2761 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG 2821 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 2881 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 2941 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 3001 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 3061 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA 3121 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 3181 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT 3241 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT 3301 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA 3361 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 3421 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 3481 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 3541 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 3601 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 3661 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 3721 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 3781 ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 3841 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 3901 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 3961 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 4021 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 4081 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 4141 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC 4201 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 4261 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 4321 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 4381 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt ttaaacatga 4441 tcttactaac taactattct catttaaatt ttcagAGCTT AAAAATGGCT GAAATCACTC 4501 ACAACGATGG ATACGCTAAC AACTTGGAAA TGAAAT // 1493 LOCUS ADDGENE_1493 4530 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_73 complete plasmid sequence KEYWORDS . SOURCE pPD95_73 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1493 at www.addgene.org FEATURES Location/Qualifiers source 1..4530 /organism="Addgene plasmid pPD95_73" /mol_type="other DNA" CDS 9..1046 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMT APKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSA MPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGI DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRH NIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITHGMDELYK" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" misc_feature 135..161 /label="SV40.NLS" gene 177..1037 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 348..398 /label="SynIVS.R" misc_feature 420..439 /label="gfp.S65C" misc_feature 553..603 /label="SynIVS.S" misc_feature 768..818 /label="SynIVS.T" misc_feature 1132..1182 /label="SynIVS.L" promoter 2310..2338 /label="Amp_promoter" CDS 2380..3240 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2380..3240 /label="Ampicillin" /gene="Ampicillin" rep_origin 3395..4014 /label="pBR322_origin" misc_feature 4230..4256 /label="lacI.C" promoter 4323..4352 /label="lac_promoter" misc_feature 4387..4405 /label="M13r_primer" misc_feature 4419..4469 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGAGCTCAGA AAAAATGACT GCTCCAAAGA AGAAGCGTAA GGTACCGGTA GAAAAAATGA 181 GTAAAGGAGA AGAACTTTTC ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG 241 TTAATGGGCA CAAATTTTCT GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC 301 TTACCCTTAA ATTTATTTGC ACTACTGGAA AACTACCTGT TCCATGGgta agtttaaaca 361 tatatatact aactaaccct gattatttaa attttcagCC AACACTTGTC ACTACTTTCT 421 gTTATGGTGT TCAATGCTTc TCgAGATACC CAGATCATAT GAAACgGCAT GACTTTTTCA 481 AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA 541 ACTACAAGAC ACgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt 601 cagGTGCTGA AGTCAAGTTT GAAGGTGATA CCCTTGTTAA TAGAATCGAG TTAAAAGGTA 661 TTGATTTTAA AGAAGATGGA AACATTCTTG GACACAAATT GGAATACAAC TATAACTCAC 721 ACAATGTATA CATCATGGCA GACAAACAAA AGAATGGAAT CAAAGTTgta agtttaaaca 781 tgattttact aactaactaa tctgatttaa attttcagAA CTTCAAAATT AGACACAACA 841 TTGAAGATGG AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG 901 GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC 961 CCAACGAAAA GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC 1021 ATGGCATGGA TGAACTATAC AAATAGCATT CGTAGAATTC CAACTGAGCG CCGGTCGCTA 1081 CCATTACCAA CTTGTCTGGT GTCAAAAATA ATAGGGGCCG CTGTCATCAG Agtaagttta 1141 aactgagttc tactaactaa cgagtaatat ttaaattttc agCATCTCGC GCCCGTGCCT 1201 CTGACTTCTA AGTCCAATTA CTCTTCAACA TCCCTACATG CTCTTTCTCC CTGTGCTCCC 1261 ACCCCCTATT TTTGTTATTA TCAAAAAACT TCTCTTAATT TCTTTGTTTT TTAGCTTCTT 1321 TTAAGTCACC TCTAACAATG AAATTGTGTA GATTCAAAAA TAGAATTAAT TCGTAATAAA 1381 AAGTCGAAAA AAATTGTGCT CCCTCCCCCC ATTAATAATA ATTCTATCCC AAAATCTACA 1441 CAATGTTCTG TGTACACTTC TTATGTTTTT TACTTCTGAT AAATTTTTTT GAAACATCAT 1501 AGAAAAAACC GCACACAAAA TACCTTATCA TATGTTACGT TTCAGTTTAT GACCGCAATT 1561 TTTATTTCTT CGCACGTCTG GGCCTCTCAT GACGTCAAAT CATGCTCATC GTGAAAAAGT 1621 TTTGGAGTAT TTTTGGAATT TTTCAATCAA GTGAAAGTTT ATGAAATTAA TTTTCCTGCT 1681 TTTGCTTTTT GGGGTTTCCC CTATTGTTTG TCAAGATTTC GAGGACGGCG TTTTTCTTGC 1741 TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC GGAAGAAGGT TTGGGTTTGA 1801 GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT GGAGTAGTGT CTATGGGGTT 1861 TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC ATAACTGTTT CCTACTAGTC 1921 GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA 1981 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC 2041 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG 2101 AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA 2161 GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 2221 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 2281 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 2341 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 2401 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 2461 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 2521 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 2581 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 2641 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 2701 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 2761 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 2821 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 2881 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 2941 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 3001 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 3061 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 3121 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 3181 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 3241 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 3301 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 3361 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 3421 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 3481 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 3541 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 3601 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 3661 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 3721 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 3781 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 3841 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 3901 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 3961 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 4021 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 4081 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 4141 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 4201 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 4261 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 4321 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 4381 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt aagtttaaac atgatcttac 4441 taactaacta ttctcattta aattttcagA GCTTAAAAAT GGCTGAAATC ACTCACAACG 4501 ATGGATACGC TAACAACTTG GAAATGAAAT // 1494 LOCUS ADDGENE_1494 4487 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_75 complete plasmid sequence KEYWORDS . SOURCE pPD95_75 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1494 at www.addgene.org FEATURES Location/Qualifiers source 1..4487 /organism="Addgene plasmid pPD95_75" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" gene 134..994 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 134..1003 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" misc_feature 305..355 /label="SynIVS.R" misc_feature 377..396 /label="gfp.S65C" misc_feature 510..560 /label="SynIVS.S" misc_feature 725..775 /label="SynIVS.T" misc_feature 1089..1139 /label="SynIVS.L" promoter 2267..2295 /label="Amp_promoter" gene 2337..3197 /label="Ampicillin" /gene="Ampicillin" CDS 2337..3197 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3352..3971 /label="pBR322_origin" misc_feature 4187..4213 /label="lacI.C" promoter 4280..4309 /label="lac_promoter" misc_feature 4344..4362 /label="M13r_primer" misc_feature 4376..4426 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGGTAGAA AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT 181 TGAATTAGAT GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA 241 TGCAACATAC GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC 301 ATGGgtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC 361 ACTTGTCACT ACTTTCTgTT ATGGTGTTCA ATGCTTcTCg AGATACCCAG ATCATATGAA 421 ACgGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT 481 TTTCAAAGAT GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc 541 atacatattt aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG 601 AATCGAGTTA AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA 661 ATACAACTAT AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA 721 AGTTgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT 781 CAAAATTAGA CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA 841 TACTCCAATT GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC 901 TGCCCTTTCG AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC 961 AGCTGCTGGG ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTCCAA 1021 CTGAGCGCCG GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG 1081 TCATCAGAgt aagtttaaac tgagttctac taactaacga gtaatattta aattttcagC 1141 ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC 1201 TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT 1261 TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG 1321 AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT 1381 CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA 1441 TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC 1501 AGTTTATGAC CGCAATTTTT ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT 1561 GCTCATCGTG AAAAAGTTTT GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG 1621 AAATTAATTT TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG 1681 GACGGCGTTT TTCTTGCTAA AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA 1741 AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA 1801 GTAGTGTCTA TGGGGTTTTT GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA 1861 ACTGTTTCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG 1921 TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC 1981 CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT 2041 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC 2101 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC 2161 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC 2221 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 2281 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 2341 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 2401 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 2461 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 2521 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 2581 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 2641 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 2701 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 2761 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 2821 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 2881 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 2941 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 3001 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 3061 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 3121 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 3181 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 3241 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 3301 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 3361 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 3421 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 3481 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 3541 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 3601 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 3661 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 3721 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC 3781 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 3841 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 3901 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 3961 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 4021 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 4081 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 4141 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 4201 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 4261 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 4321 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTgtaag 4381 tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagAGCT TAAAAATGGC 4441 TGAAATCACT CACAACGATG GATACGCTAA CAACTTGGAA ATGAAAT // 1495 LOCUS ADDGENE_1495 4486 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_77 complete plasmid sequence KEYWORDS . SOURCE pPD95_77 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1495 at www.addgene.org FEATURES Location/Qualifiers source 1..4486 /organism="Addgene plasmid pPD95_77" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..121 /label="rf22.04" CDS 133..1002 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 133..993 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 304..354 /label="SynIVS.R" misc_feature 376..395 /label="gfp.S65C" misc_feature 509..559 /label="SynIVS.S" misc_feature 724..774 /label="SynIVS.T" misc_feature 1088..1138 /label="SynIVS.L" promoter 2266..2294 /label="Amp_promoter" CDS 2336..3196 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2336..3196 /label="Ampicillin" /gene="Ampicillin" rep_origin 3351..3970 /label="pBR322_origin" misc_feature 4186..4212 /label="lacI.C" promoter 4279..4308 /label="lac_promoter" misc_feature 4343..4361 /label="M13r_primer" misc_feature 4375..4425 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGAGGGTA 121 CCGGTAGAAA AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT 181 GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT 241 GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA 301 TGGgtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA 361 CTTGTCACTA CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA 421 CgGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT 481 TTCAAAGATG ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca 541 tacatattta aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA 601 ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA 661 TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA 721 GTTgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC 781 AAAATTAGAC ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT 841 ACTCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT 901 GCCCTTTCGA AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA 961 GCTGCTGGGA TTACACATGG CATGGATGAA CTATACAAAT AGCATTCGTA GAATTCCAAC 1021 TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT 1081 CATCAGAgta agtttaaact gagttctact aactaacgag taatatttaa attttcagCA 1141 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 1201 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 1261 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 1321 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 1381 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 1441 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 1501 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 1561 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 1621 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 1681 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 1741 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 1801 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 1861 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 1921 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 1981 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 2041 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 2101 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 2161 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 2221 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 2281 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 2341 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 2401 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 2461 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 2521 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 2581 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 2641 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 2701 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 2761 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 2821 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 2881 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 2941 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 3001 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 3061 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 3121 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 3181 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 3241 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 3301 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3361 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3421 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3481 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 3541 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3601 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3661 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3721 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 3781 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 3841 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 3901 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 3961 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 4021 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 4081 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 4141 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 4201 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 4261 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 4321 GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTgtaagt 4381 ttaaacatga tcttactaac taactattct catttaaatt ttcagAGCTT AAAAATGGCT 4441 GAAATCACTC ACAACGATGG ATACGCTAAC AACTTGGAAA TGAAAT // 1496 LOCUS ADDGENE_1496 4491 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_79 complete plasmid sequence KEYWORDS . SOURCE pPD95_79 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1496 at www.addgene.org FEATURES Location/Qualifiers source 1..4491 /organism="Addgene plasmid pPD95_79" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" CDS 138..1007 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYK" gene 138..998 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 309..359 /label="SynIVS.R" misc_feature 381..400 /label="gfp.S65C" misc_feature 514..564 /label="SynIVS.S" misc_feature 729..779 /label="SynIVS.T" misc_feature 1093..1143 /label="SynIVS.L" promoter 2271..2299 /label="Amp_promoter" CDS 2341..3201 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2341..3201 /label="Ampicillin" /gene="Ampicillin" rep_origin 3356..3975 /label="pBR322_origin" misc_feature 4191..4217 /label="lacI.C" promoter 4284..4313 /label="lac_promoter" misc_feature 4348..4366 /label="M13r_primer" misc_feature 4380..4430 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGGT AGAAAAAATG AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC 181 TTGTTGAATT AGATGGTGAT GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG 241 GTGATGCAAC ATACGGAAAA CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG 301 TTCCATGGgt aagtttaaac atatatatac taactaaccc tgattattta aattttcagC 361 CAACACTTGT CACTACTTTC TgTTATGGTG TTCAATGCTT cTCgAGATAC CCAGATCATA 421 TGAAACgGCA TGACTTTTTC AAGAGTGCCA TGCCCGAAGG TTATGTACAG GAAAGAACTA 481 TATTTTTCAA AGATGACGGG AACTACAAGA CACgtaagtt taaacagttc ggtactaact 541 aaccatacat atttaaattt tcagGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA 601 ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAT 661 TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA AAGAATGGAA 721 TCAAAGTTgt aagtttaaac atgattttac taactaacta atctgattta aattttcagA 781 ACTTCAAAAT TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC 841 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCCACAC 901 AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA CATGGTCCTT CTTGAGTTTG 961 TAACAGCTGC TGGGATTACA CATGGCATGG ATGAACTATA CAAATAGCAT TCGTAGAATT 1021 CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG TGTCAAAAAT AATAGGGGCC 1081 GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt 1141 cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT ACTCTTCAAC ATCCCTACAT 1201 GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT ATCAAAAAAC TTCTCTTAAT 1261 TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT GAAATTGTGT AGATTCAAAA 1321 ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC TCCCTCCCCC CATTAATAAT 1381 AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT CTTATGTTTT TTACTTCTGA 1441 TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA ATACCTTATC ATATGTTACG 1501 TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT GGGCCTCTCA TGACGTCAAA 1561 TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT TTTTCAATCA AGTGAAAGTT 1621 TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC CCTATTGTTT GTCAAGATTT 1681 CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT GAGCACGATG CAAGAAAGAT 1741 CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT AGAAGTTGAT AATTTGAAAG 1801 TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA TACATTCCCA ATATACCAAA 1861 CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG TCTCGCGCGT TTCGGTGATG 1921 ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG 1981 ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT 2041 GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA 2101 TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG GCCTTAAGGG CCTCGTGATA 2161 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT 2221 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG 2281 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT 2341 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT 2401 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA 2461 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC 2521 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC 2581 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG 2641 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA 2701 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC 2761 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT 2821 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG 2881 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT 2941 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC 3001 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT 3061 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC 3121 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC 3181 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT 3241 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG 3301 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC 3361 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA 3421 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG 3481 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA 3541 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA 3601 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG 3661 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG 3721 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG 3781 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG 3841 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC 3901 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA 3961 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG 4021 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 4081 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA 4141 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 4201 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG 4261 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA 4321 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTg 4381 taagtttaaa catgatctta ctaactaact attctcattt aaattttcag AGCTTAAAAA 4441 TGGCTGAAAT CACTCACAAC GATGGATACG CTAACAACTT GGAAATGAAA T // 1497 LOCUS ADDGENE_1497 4485 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_81 complete plasmid sequence KEYWORDS . SOURCE pPD95_81 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1497 at www.addgene.org FEATURES Location/Qualifiers source 1..4485 /organism="Addgene plasmid pPD95_81" /mol_type="other DNA" CDS 9..1001 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MPAGRLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPVEKMSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLN IYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEY NYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQ NTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 19..39 /label="rf81.34" misc_feature 60..101 /label="SynIVS.A" gene 132..992 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 303..353 /label="SynIVS.R" misc_feature 375..394 /label="gfp.S65C" misc_feature 508..558 /label="SynIVS.S" misc_feature 723..773 /label="SynIVS.T" misc_feature 1087..1137 /label="SynIVS.L" promoter 2265..2293 /label="Amp_promoter" CDS 2335..3195 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2335..3195 /label="Ampicillin" /gene="Ampicillin" rep_origin 3350..3969 /label="pBR322_origin" misc_feature 4185..4211 /label="lacI.C" promoter 4278..4307 /label="lac_promoter" misc_feature 4342..4360 /label="M13r_primer" misc_feature 4374..4424 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTAGAGG ATCCCCGGGA TTGGCCAAAG GACCCAAAGg 61 tatgtttcga atgatactaa cataacatag aacattttca gGAGGACCCT TGGAGGGTAC 121 CGGTAGAAAA AATGAGTAAA GGAGAAGAAC TTTTCACTGG AGTTGTCCCA ATTCTTGTTG 181 AATTAGATGG TGATGTTAAT GGGCACAAAT TTTCTGTCAG TGGAGAGGGT GAAGGTGATG 241 CAACATACGG AAAACTTACC CTTAAATTTA TTTGCACTAC TGGAAAACTA CCTGTTCCAT 301 GGgtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagCCAACAC 361 TTGTCACTAC TTTCTgTTAT GGTGTTCAAT GCTTcTCgAG ATACCCAGAT CATATGAAAC 421 gGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT ACAGGAAAGA ACTATATTTT 481 TCAAAGATGA CGGGAACTAC AAGACACgta agtttaaaca gttcggtact aactaaccat 541 acatatttaa attttcagGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT GTTAATAGAA 601 TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC AAATTGGAAT 661 ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT GGAATCAAAG 721 TTgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagAACTTCA 781 AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT CAACAAAATA 841 CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC ACACAATCTG 901 CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG TTTGTAACAG 961 CTGCTGGGAT TACACATGGC ATGGATGAAC TATACAAATA GCATTCGTAG AATTCCAACT 1021 GAGCGCCGGT CGCTACCATT ACCAACTTGT CTGGTGTCAA AAATAATAGG GGCCGCTGTC 1081 ATCAGAgtaa gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagCAT 1141 CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT 1201 TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT 1261 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA 1321 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT 1381 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT 1441 TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT TACGTTTCAG 1501 TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT CAAATCATGC 1561 TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA AGTTTATGAA 1621 ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG ATTTCGAGGA 1681 CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA AGATCGGAAG 1741 AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG AAAGTGGAGT 1801 AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC CAAACATAAC 1861 TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG 1921 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG 1981 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA 2041 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 2101 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT GATACGCCTA 2161 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 2221 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 2281 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 2341 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 2401 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 2461 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 2521 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 2581 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 2641 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 2701 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 2761 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 2821 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 2881 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 2941 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 3001 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 3061 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 3121 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 3181 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 3241 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 3301 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 3361 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 3421 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 3481 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 3541 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 3601 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 3661 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 3721 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC 3781 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 3841 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 3901 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 3961 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 4021 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 4081 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 4141 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 4201 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 4261 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 4321 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTgtaagtt 4381 taaacatgat cttactaact aactattctc atttaaattt tcagAGCTTA AAAATGGCTG 4441 AAATCACTCA CAACGATGGA TACGCTAACA ACTTGGAAAT GAAAT // 1498 LOCUS ADDGENE_1498 4463 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_85 complete plasmid sequence KEYWORDS . SOURCE pPD95_85 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1498 at www.addgene.org FEATURES Location/Qualifiers source 1..4463 /organism="Addgene plasmid pPD95_85" /mol_type="other DNA" CDS 1..1113 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTMITPSLHACRSTLEDPRDWPKDPKVCFE*Y*HNIEHFQEDPW LAKMHKVLLALFFIFLAPASALAVSEPVLASTVPVEKMSKGEELFTGVVPILVELDGD VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVT TFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PY IFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI KVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLS TQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 79..120 /label="SynIVS.A" misc_feature 142..207 /label="ATG-SecSig" gene 244..1104 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 415..465 /label="SynIVS.R" misc_feature 487..506 /label="gfp.S65T" misc_feature 620..670 /label="SynIVS.S" misc_feature 835..885 /label="SynIVS.T" misc_feature 1199..1249 /label="SynIVS.L" promoter 2377..2405 /label="Amp_promoter" CDS 2447..3307 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2447..3307 /label="Ampicillin" /gene="Ampicillin" rep_origin 3462..4081 /label="pBR322_origin" misc_feature 4297..4323 /label="lacI.C" promoter 4390..4419 /label="lac_promoter" misc_feature 4454..4472 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGAT 61 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 121 GAGGACCCTT GGCTAGCAAA AATGCATAAG GTTTTGCTGG CACTGTTCTT TATCTTTCTG 181 GCACCAGCAT CCGCACTGGC AGTCTCCGAA CCGGTCCTAG CGTCGACGGT ACCGGTAGAA 241 AAAATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT 301 GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC 361 GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGgtaagt 421 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagCCAAC ACTTGTCACT 481 ACTTTCaCTT ATGGTGTTCA ATGCTTcTCg AGATACCCAG ATCATATGAA ACgGCATGAC 541 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAA GAACTATATT TTTCAAAGAT 601 GACGGGAACT ACAAGACACg taagtttaaa cagttcggta ctaactaacc atacatattt 661 aaattttcag GTGCTGAAGT CAAGTTTGAA GGTGATACCC TTGTTAATAG AATCGAGTTA 721 AAAGGTATTG ATTTTAAAGA AGATGGAAAC ATTCTTGGAC ACAAATTGGA ATACAACTAT 781 AACTCACACA ATGTATACAT CATGGCAGAC AAACAAAAGA ATGGAATCAA AGTTgtaagt 841 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagAACTT CAAAATTAGA 901 CACAACATTG AAGATGGAAG CGTTCAACTA GCAGACCATT ATCAACAAAA TACTCCAATT 961 GGCGATGGCC CTGTCCTTTT ACCAGACAAC CATTACCTGT CCACACAATC TGCCCTTTCG 1021 AAAGATCCCA ACGAAAAGAG AGACCACATG GTCCTTCTTG AGTTTGTAAC AGCTGCTGGG 1081 ATTACACATG GCATGGATGA ACTATACAAA TAGCATTCGT AGAATTCCAA CTGAGCGCCG 1141 GTCGCTACCA TTACCAACTT GTCTGGTGTC AAAAATAATA GGGGCCGCTG TCATCAGAgt 1201 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagC ATCTCGCGCC 1261 CGTGCCTCTG ACTTCTAAGT CCAATTACTC TTCAACATCC CTACATGCTC TTTCTCCCTG 1321 TGCTCCCACC CCCTATTTTT GTTATTATCA AAAAACTTCT CTTAATTTCT TTGTTTTTTA 1381 GCTTCTTTTA AGTCACCTCT AACAATGAAA TTGTGTAGAT TCAAAAATAG AATTAATTCG 1441 TAATAAAAAG TCGAAAAAAA TTGTGCTCCC TCCCCCCATT AATAATAATT CTATCCCAAA 1501 ATCTACACAA TGTTCTGTGT ACACTTCTTA TGTTTTTTAC TTCTGATAAA TTTTTTTGAA 1561 ACATCATAGA AAAAACCGCA CACAAAATAC CTTATCATAT GTTACGTTTC AGTTTATGAC 1621 CGCAATTTTT ATTTCTTCGC ACGTCTGGGC CTCTCATGAC GTCAAATCAT GCTCATCGTG 1681 AAAAAGTTTT GGAGTATTTT TGGAATTTTT CAATCAAGTG AAAGTTTATG AAATTAATTT 1741 TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA TTGTTTGTCA AGATTTCGAG GACGGCGTTT 1801 TTCTTGCTAA AATCACAAGT ATTGATGAGC ACGATGCAAG AAAGATCGGA AGAAGGTTTG 1861 GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA GTTGATAATT TGAAAGTGGA GTAGTGTCTA 1921 TGGGGTTTTT GCCTTAAATG ACAGAATACA TTCCCAATAT ACCAAACATA ACTGTTTCCT 1981 ACTAGTCGGC CGTACGGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC 2041 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA 2101 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG 2161 GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC 2221 GTAAGGAGAA AATACCGCAT CAGGCGGCCT TAAGGGCCTC GTGATACGCC TATTTTTATA 2281 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT 2341 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 2401 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA 2461 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 2521 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 2581 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC 2641 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 2701 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC 2761 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 2821 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA 2881 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC 2941 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 3001 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT 3061 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 3121 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 3181 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA 3241 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 3301 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT 3361 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA 3421 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3481 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3541 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3601 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3661 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3721 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3781 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3841 CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG 3901 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3961 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 4021 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 4081 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT 4141 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG 4201 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG 4261 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC 4321 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC 4381 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA 4441 ACAATTTCAC ACAGGAAACA GCT // 1499 LOCUS ADDGENE_1499 5782 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_86 complete plasmid sequence KEYWORDS . SOURCE pPD95_86 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1499 at www.addgene.org FEATURES Location/Qualifiers source 1..5782 /organism="Addgene plasmid pPD95_86" /mol_type="other DNA" misc_feature 36..59 /label="myo3.-2341" misc_feature 2355..2378 /label="myo3.Tsa" misc_feature 2451..2492 /label="SynIVS.A" misc_feature 2499..2516 /label="rf21.28" promoter 3696..3724 /label="Amp_promoter" CDS 3766..4626 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3766..4626 /label="Ampicillin" /gene="Ampicillin" rep_origin 4781..5400 /label="pBR322_origin" misc_feature 5616..5642 /label="lacI.C" promoter 5709..5738 /label="lac_promoter" misc_feature 5773..5791 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGGCTA TAATAAGTTC TTGAATAAAA 61 TAATTTTCCC GACAAAACAT GAGTATTTCT TTCGAAAATA AAAGTGCAGG CTAATTAGAG 121 ATTATTCTGT AATTAACTGC ATAATTTGTC ACGTGCCATA GTTTTACATT CCACTACGTC 181 ATAGTTCTTA AAATACTAAT CTCCTGAAAA TAGAAGTAGG TGAAGAAAGT TTAATTATCA 241 GTTCTAAAAT GACAATTGAT CTTTGGAATA TGTTCTGAAA CTACCGATCA TTGAACAGAT 301 GCTATTTGAA TGATATAGAA TTGTATATTT GCAATTTCTG AAACGCGTTC TTAAAGGCAC 361 ACAGATTAAT TCAAAAGGGT CTGGCCGCAA AAAGGTTTAT GGTGGCCGAT TTTGAGTTTT 421 GTGTGTGATT GCTTTTTCAC AATCAGTGTT TTCAGGATTA TGTGATGAAC TAGATCTTCA 481 AGTTTCGTTA CATTTCATAT GTTTTCGGAA CTCACGAAGT ACATATTGGG TATTGTGCTC 541 AAAAAATTCA GCAATCAGCT TCGCTCCGCT GACTTTAGAA CCCAAAAAAA TAGTATGGCC 601 AAACTGACTG TGTTACGATC ATTTCAATTT TTCAATACAT ATTTAAGATT TCTAAGAGTA 661 AGAAGGTCAA AAACTGTTCT GGAATACATA TATATTTTTC AGGTTACAAT TAGTCAAAAA 721 GTGCACTGAA ATATACGTTT TAATTTCACG AATAACCCAA TTAGTTCAAT GTATTTTTGG 781 TCAACCAACG TTAAAGTTTG GCTTCCAACC AATTATCATT TCTGATCAAC CACAATGTTT 841 TTTCTTTATC TGCAAGTTAA TTTTTTATTT TTATCCAGAT GTTTGGCATA TTTTTCAATT 901 CTTCACTAGC GCCCACTTCT TGCACTTCCG GCGCCCTGAA TCTAATGCAT CTGTTGCAAG 961 AATTGAAAGA CCAATCAACA CATTGTTTTC TTCACGAGAT ACTGAAGAAA ATGAATAAAA 1021 ACAGAGAAAA AGAGCCATGT GATTAGTGAC AACTGTTGCT AACAGATACC ATAGCTTGGA 1081 CTTGGTACGT GATGGCAACG TATGGGTCAA CAAAAATGAT TGCAGAGGGG GTGCAAAACA 1141 GTCAAGTCGA GAAAATATGA AAAACAGAAA ACAAAGAACA GAAAAATGGG TTTGAGAGTC 1201 AGTATAATTT ATAAAAGAAA AATTGTACAT AGAAATTAAC CATTTTTGTA GAAGAAGTTA 1261 TTTTTCAAGC ATCGTTAAAA ATTATTCAAA GCACCTTATT TCATATTTAA TTTTAAACAT 1321 GGTTAAATGA ACAACACGGT GCGCAATCAG GAAAACTTGA AATCTGAAAC TGTTGTTGTG 1381 ATCTTCTTCG CAACTGTTCA GATAGCACTA GTGTAATGTT AAGAGTGCGC GAATATAATG 1441 GAATATAATG GATCACACCT CCTGCCATCA GGTAAACGTC TCTGTTATCA CATATTTCCA 1501 ACTATTAAAT TTTTACCTTT TACAGTTTTA CATTTTTTTG AAAAAAGTAA CTTTTTGTCT 1561 TCAAAATCCC TGACGAAAAT ATCAAATATT TTAATCGAGA CTGCAGAGGA ACCGATTGAT 1621 GATTTGGAAA ATCCAGCTTT ACCTGTGTAA GAACTGAAAA GTTTCATAAC CCTAGGGTAT 1681 TCCCAGTTAC ATTCCCCACT GGCTAACAAT AGCACCCAGT TTTTCATCAC CTTTCTTCAA 1741 ATTTCTCGGC GATTTGTTAA AAACAAAATT TGTGTCCCTT CTCTGATATC TCTATGTCTC 1801 TAAACACAAG TTCATCGGAA AACGAAGGAG GGTAGGTGTT GGTTGGGCTC CCGAAGTGAA 1861 AATAGAAGAG CAAGAATAGA ATATTAGAGA GAGAGTGCAG AGAGGGCGGG ATAGCTCCCG 1921 GGATTCCGTT TTCTTCTTCT TTATCTTCAA CGATGATGTG TGTGCGTGTT GTATAGATTC 1981 TGTTGCTCCC CCACAACTCG CTCCGAAGGC TCAATACAAT TCAATTGATA TTGGAGGAGA 2041 GCCTACCGGA GTGGGAGGAT AAGAAGAAAC ATAAGAAGAA GAAGAAGAAG AAGCATGCTT 2101 CTGGTTTTTG ATGCTATGAA AACGGCACAA AAAGATGATT GAGGTCCCTT TTCAATACCT 2161 TCTCTCATCT TTCAAATCCC ATTGAAACCT AAAACTTCTC ACCACGCTTT ACCATTGTTC 2221 TCCAAAAACT TATAGCAATG TCTATAACTT TTTTATCTCT GAAAAGCAGT GTTCCATTTT 2281 TCTTTTTCCT ATTTTATTTC AATTGTTTCT CACATTTCGT TTGGATTCTT TGCTTGTCAA 2341 CCAGCTTCTT CTTCCACTTT TACCGTCTAA TTTTCAGGGC AGGGAGCCAT CAAACCCACG 2401 ACCACTAGAT CCATCTAGAG GATCCCCGGG ATTGGCCAAA GGACCCAAAG gtatgtttcg 2461 aatgatacta acataacata gaacattttc agGAGGACCC TTGGCTAGCG TCGACGGTAC 2521 CATGGTATTG ATATCTGAGC TCCGCATCGG CCGCTGTCAT CAGATCGCCA TCTCGCGCCC 2581 GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT 2641 GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG 2701 CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT 2761 AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA 2821 TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA 2881 CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC 2941 GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA 3001 AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT 3061 CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG ACGGCGTTTT 3121 TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG 3181 GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT 3241 GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA 3301 CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT 3361 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC 3421 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG 3481 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG 3541 TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG 3601 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 3661 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 3721 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 3781 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 3841 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 3901 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 3961 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 4021 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 4081 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 4141 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG 4201 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 4261 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 4321 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 4381 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 4441 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA 4501 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 4561 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT 4621 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT 4681 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA 4741 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 4801 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 4861 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 4921 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 4981 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 5041 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 5101 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 5161 ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 5221 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 5281 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 5341 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 5401 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 5461 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 5521 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC 5581 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 5641 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 5701 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 5761 CAATTTCACA CAGGAAACAG CT // 1500 LOCUS ADDGENE_1500 7661 bp DNA circular SYN 01/03/2005 DEFINITION pPD95_93 complete plasmid sequence KEYWORDS . SOURCE pPD95_93 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1500 at www.addgene.org FEATURES Location/Qualifiers source 1..7661 /organism="Addgene plasmid pPD95_93" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" misc_feature 377..403 /label="SV40.NLS" CDS 377..4423 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPE AVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSL TFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENR LAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLE AEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPK LWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRH EHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANI ETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYR WIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETR PLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYG GDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHS DNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNA TAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSG FLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAA LLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGG DDSWSPSVSAEFQLSAGRYHYQLVWCQK" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65C" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4420 /label="lacZ" /gene="lacZ" promoter 5575..5603 /label="Amp_promoter" gene 5645..6505 /label="Ampicillin" /gene="Ampicillin" CDS 5645..6505 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 6660..7279 /label="pBR322_origin" misc_feature 7495..7521 /label="lacI.C" promoter 7588..7617 /label="lac_promoter" misc_feature 7652..7670 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGCAGC CTGAATGGCG AATGGCGCTT TGCCTGGTTT CCGGCACCAG AAGCGGTGCC 1561 GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA GGCCGATACT GTCGTCGTCC CCTCAAACTG 1621 GCAGATGCAC GGTTACGATG CGCCCATCTA CACCAACGTG ACCTATCCCA TTACGGTCAA 1681 TCCGCCGTTT GTTCCCACGG AGAATCCGAC GGGTTGTTAC TCGCTCACAT TTAATGTTGA 1741 TGAAAGCTGG CTACAGGAAG GCCAGACGCG AATTATTTTT GATGGCGTTA ACTCGGCGTT 1801 TCATCTGTGG TGCAACGGGC GCTGGGTCGG TTACGGCCAG GACAGTCGTT TGCCGTCTGA 1861 ATTTGACCTG AGCGCATTTT TACGCGCCGG AGAAAACCGC CTCGCGGTGA TGGTGCTGCG 1921 CTGGAGTGAC GGCAGTTATC TGGAAGATCA GGATATGTGG CGGATGAGCG GCATTTTCCG 1981 TGACGTCTCG TTGCTGCATA AACCGACTAC ACAAATCAGC GATTTCCATG TTGCCACTCG 2041 CTTTAATGAT GATTTCAGCC GCGCTGTACT GGAGGCTGAA GTTCAGATGT GCGGCGAGTT 2101 GCGTGACTAC CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG 2161 CACCGCGCCT TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC 2221 ACTACGTCTG AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG 2281 TGCGGTGGTT GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT 2341 CGGTTTCCGC GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT 2401 GATTCGAGGC GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA 2461 GACGATGGTG CAGGATATCC TGCTGATGAA GCAGAACAAC TTTAACGCCG TGCGCTGTTC 2521 GCATTATCCG AACCATCCGC TGTGGTACAC GCTGTGCGAC CGCTACGGCC TGTATGTGGT 2581 GGATGAAGCC AATATTGAAA CCCACGGCAT GGTGCCAATG AATCGTCTGA CCGATGATCC 2641 GCGCTGGCTA CCGGCGATGA GCGAACGCGT AACGCGAATG GTGCAGCGCG ATCGTAATCA 2701 CCCGAGTGTG ATCATCTGGT CGCTGGGGAA TGAATCAGGC CACGGCGCTA ATCACGACGC 2761 GCTGTATCGC TGGATCAAAT CTGTCGATCC TTCCCGCCCG GTGCAGTATG AAGGCGGCGG 2821 AGCCGACACC ACGGCCACCG ATATTATTTG CCCGATGTAC GCGCGCGTGG ATGAAGACCA 2881 GCCCTTCCCG GCTGTGCCGA AATGGTCCAT CAAAAAATGG CTTTCGCTAC CTGGAGAGAC 2941 GCGCCCGCTG ATCCTTTGCG AATACGCCCA CGCGATGGGT AACAGTCTTG GCGGTTTCGC 3001 TAAATACTGG CAGGCGTTTC GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG 3061 GGTGGATCAG TCGCTGATTA AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG 3121 TGATTTTGGC GATACGCCGA ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA 3181 CCGCACGCCG CATCCAGCGC TGACGGAAGC AAAACACCAG CAGCAGTTTT TCCAGTTCCG 3241 TTTATCCGGG CAAACCATCG AAGTGACCAG CGAATACCTG TTCCGTCATA GCGATAACGA 3301 GCTCCTGCAC TGGATGGTGG CGCTGGATGG TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT 3361 GGATGTCGCT CCACAAGGTA AACAGTTGAT TGAACTGCCT GAACTACCGC AGCCGGAGAG 3421 CGCCGGGCAA CTCTGGCTCA CAGTACGCGT AGTGCAACCG AACGCGACCG CATGGTCAGA 3481 AGCCGGGCAC ATCAGCGCCT GGCAGCAGTG GCGTCTGGCG GAAAACCTCA GTGTGACGCT 3541 CCCCGCCGCG TCCCACGCCA TCCCGCATCT GACCACCAGC GAAATGGATT TTTGCATCGA 3601 GCTGGGTAAT AAGCGTTGGC AATTTAACCG CCAGTCAGGC TTTCTTTCAC AGATGTGGAT 3661 TGGCGATAAA AAACAACTGC TGACGCCGCT GCGCGATCAG TTCACCCGTG CACCGCTGGA 3721 TAACGACATT GGCGTAAGTG AAGCGACCCG CATTGACCCT AACGCCTGGG TCGAACGCTG 3781 GAAGGCGGCG GGCCATTACC AGGCCGAAGC AGCGTTGTTG CAGTGCACGG CAGATACACT 3841 TGCTGATGCG GTGCTGATTA CGACCGCTCA CGCGTGGCAG CATCAGGGGA AAACCTTATT 3901 TATCAGCCGG AAAACCTACC GGATTGATGG TAGTGGTCAA ATGGCGATTA CCGTTGATGT 3961 TGAAGTGGCG AGCGATACAC CGCATCCGGC GCGGATTGGC CTGAACTGCC AGCTGGCGCA 4021 GGTAGCAGAG CGGGTAAACT GGCTCGGATT AGGGCCGCAA GAAAACTATC CCGACCGCCT 4081 TACTGCCGCC TGTTTTGACC GCTGGGATCT GCCATTGTCA GACATGTATA CCCCGTACGT 4141 CTTCCCGAGC GAAAACGGTC TGCGCTGCGG GACGCGCGAA TTGAATTATG GCCCACACCA 4201 GTGGCGCGGC GACTTCCAGT TCAACATCAG CCGCTACAGT CAACAGCAAC TGATGGAAAC 4261 CAGCCATCGC CATCTGCTGC ACGCGGAAGA AGGCACATGG CTGAATATCG ACGGTTTCCA 4321 TATGGGGATT GGTGGCGACG ACTCCTGGAG CCCGTCAGTA TCGGCGGAAT TCCAACTGAG 4381 CGCCGGTCGC TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC 4441 AGATCGCCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT 4501 ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT 4561 TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC 4621 AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA 4681 TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT 4741 CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA CAAAATACCT TATCATATGT 4801 TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC GTCTGGGCCT CTCATGACGT 4861 CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG GAATTTTTCA ATCAAGTGAA 4921 AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT TTCCCCTATT GTTTGTCAAG 4981 ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT TGATGAGCAC GATGCAAGAA 5041 AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT GAGTAGAAGT TGATAATTTG 5101 AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC AGAATACATT CCCAATATAC 5161 CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT TTCGTCTCGC GCGTTTCGGT 5221 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA 5281 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG 5341 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT 5401 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGGCCTTA AGGGCCTCGT 5461 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG 5521 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA 5581 TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 5641 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT 5701 TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG 5761 TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG 5821 CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT 5881 ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 5941 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA 6001 ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC 6061 GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG 6121 CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC 6181 GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 6241 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT 6301 GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG 6361 GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT 6421 CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG 6481 TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT 6541 TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT 6601 CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 6661 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 6721 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 6781 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA 6841 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 6901 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 6961 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 7021 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC 7081 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 7141 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 7201 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 7261 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 7321 CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG 7381 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC 7441 GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG 7501 CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG 7561 TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG 7621 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC T // 1501 LOCUS ADDGENE_1501 8217 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_02 complete plasmid sequence KEYWORDS . SOURCE pPD96_02 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1501 at www.addgene.org FEATURES Location/Qualifiers source 1..8217 /organism="Addgene plasmid pPD96_02" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1972 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 662..681 /label="gfp.S65C" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" gene 1376..4929 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1510..1560 /label="SynIVS.B" CDS 2011..4932 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2188..2238 /label="SynIVS.D" misc_feature 2629..2679 /label="SynIVS.E" misc_feature 2936..2986 /label="SynIVS.F" misc_feature 3217..3267 /label="SynIVS.G" misc_feature 3504..3554 /label="SynIVS.H" misc_feature 3870..3920 /label="SynIVS.I" misc_feature 4181..4231 /label="SynIVS.J" misc_feature 4587..4637 /label="SynIVS.K" misc_feature 4953..5003 /label="SynIVS.L" promoter 6131..6159 /label="Amp_promoter" gene 6201..7061 /label="Ampicillin" /gene="Ampicillin" CDS 6201..7061 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7216..7835 /label="pBR322_origin" misc_feature 8051..8077 /label="lacI.C" promoter 8144..8173 /label="lac_promoter" misc_feature 8208..8226 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1321 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1381 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1441 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1501 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1561 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1621 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1681 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1741 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1801 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1861 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1921 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1981 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 2041 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 2101 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 2161 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 2221 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2281 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2341 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2401 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2461 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2521 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2581 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2641 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2701 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2761 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2821 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2881 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2941 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 3001 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 3061 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 3121 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 3181 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3241 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3301 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3361 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3421 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3481 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3541 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3601 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3661 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3721 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3781 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3841 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3901 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3961 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 4021 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 4081 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 4141 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 4201 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4261 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4321 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4381 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4441 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4501 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4561 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4621 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4681 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4741 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4801 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4861 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4921 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4981 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 5041 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 5101 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 5161 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 5221 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5281 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5341 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5401 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5461 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5521 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5581 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5641 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5701 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5761 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5821 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5881 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5941 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 6001 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 6061 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 6121 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 6181 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6241 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6301 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6361 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6421 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6481 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6541 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6601 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6661 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6721 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6781 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6841 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6901 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6961 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 7021 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 7081 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 7141 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 7201 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7261 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7321 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7381 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7441 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7501 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7561 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7621 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7681 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7741 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7801 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7861 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7921 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7981 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 8041 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 8101 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 8161 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT // 1502 LOCUS ADDGENE_1502 8111 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_04 complete plasmid sequence KEYWORDS . SOURCE pPD96_04 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1502 at www.addgene.org FEATURES Location/Qualifiers source 1..8111 /organism="Addgene plasmid pPD96_04" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 137..163 /label="SV40.NLS" CDS 137..1732 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 179..1039 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 350..400 /label="SynIVS.R" misc_feature 422..441 /label="gfp.S65C" misc_feature 555..605 /label="SynIVS.S" misc_feature 770..820 /label="SynIVS.T" gene 1136..4689 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1270..1320 /label="SynIVS.B" CDS 1771..4692 /label="Orf0" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1948..1998 /label="SynIVS.D" misc_feature 2389..2439 /label="SynIVS.E" misc_feature 2696..2746 /label="SynIVS.F" misc_feature 2977..3027 /label="SynIVS.G" misc_feature 3264..3314 /label="SynIVS.H" misc_feature 3630..3680 /label="SynIVS.I" misc_feature 3941..3991 /label="SynIVS.J" misc_feature 4347..4397 /label="SynIVS.K" misc_feature 4713..4763 /label="SynIVS.L" promoter 5891..5919 /label="Amp_promoter" CDS 5961..6821 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5961..6821 /label="Ampicillin" /gene="Ampicillin" rep_origin 6976..7595 /label="pBR322_origin" misc_feature 7811..7837 /label="lacI.C" promoter 7904..7933 /label="lac_promoter" misc_feature 7968..7986 /label="M13r_primer" misc_feature 8000..8050 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 181 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 241 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 301 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 361 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 421 CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 481 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 541 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 601 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 661 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 721 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 781 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 841 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 901 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 961 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1021 ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC AGCACCTCGA 1081 ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG ATCCCGTCGT 1141 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1201 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1261 GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca taattttcag 1321 CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC CGGAAAGCTG 1381 GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT GGCAGATGCA 1441 CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA ATCCGCCGTT 1501 TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG ATGAAAGCTG 1561 GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT TTCATCTGTG 1621 GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga taCTAACTAA 1681 CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT GAGCGCATTT 1741 TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA CGGCAGTTAT 1801 CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC GTTGCTGCAT 1861 AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA TGATTTCtcc 1921 CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact aactaacatg 1981 ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT AACAGTTTCT 2041 TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG TGAAATTATC 2101 GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA AAACCCGAAA 2161 CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA CACCGCCGAC 2221 GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG GATTGAAAAT 2281 GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG TCACGAGCAT 2341 CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt aagtttaaac 2401 tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT GAAGCAGAAC 2461 AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA CACGCTGTGC 2521 GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG CATGGTGCCA 2581 ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG CGTAACGCGA 2641 ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG GAATGgtaag 2701 tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC AGGCCACGGC 2761 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 2821 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 2881 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 2941 CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag aactctacta 3001 actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG TCTTGGCGGT 3061 TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG 3121 GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3181 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT 3241 GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact aacgtagata 3301 atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA GTTCCGTTTA 3361 TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA TAACGAGCTC 3421 CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT GCCTCTGGAT 3481 GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC GGAGAGCGCC 3541 GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG GTCAGAAGCC 3601 GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta ctaactaact 3661 ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC CGCCGCGTCC 3721 CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT GGGTAATAAG 3781 CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG CGATAAAAAA 3841 CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA CGACATTGGC 3901 GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa acaaagttgt 3961 actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG CCATTACCAG 4021 GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT GCTGATTACG 4081 ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA AACCTACCGG 4141 ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG CGATACACCG 4201 CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG GGTAAACTGG 4261 CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG TTTTGACCGC 4321 TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta actaacatgt 4381 ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 4441 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 4501 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 4561 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 4621 CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA ACTTGTCTGG 4681 TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt ctactaacta 4741 acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT AAGTCCAATT 4801 ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT TTTTGTTATT 4861 ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC CTCTAACAAT 4921 GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA AAAATTGTGC 4981 TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT GTGTACACTT 5041 CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC CGCACACAAA 5101 ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT TCGCACGTCT 5161 GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA TTTTTGGAAT 5221 TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT TGGGGTTTCC 5281 CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC AAGTATTGAT 5341 GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG GAAGGTGAGT 5401 AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA AATGACAGAA 5461 TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG GGCCCTTTCG 5521 TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT 5581 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG 5641 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 5701 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 5761 GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 5821 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 5881 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 5941 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 6001 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 6061 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 6121 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 6181 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 6241 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 6301 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 6361 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 6421 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 6481 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 6541 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 6601 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 6661 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 6721 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 6781 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 6841 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 6901 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 6961 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 7021 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 7081 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 7141 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 7201 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 7261 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 7321 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 7381 AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 7441 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 7501 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 7561 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 7621 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 7681 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 7741 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC 7801 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA 7861 ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC 7921 CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG 7981 ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta ctaactaact attctcattt 8041 aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC GATGGATACG CTAACAACTT 8101 GGAAATGAAA T // 1503 LOCUS ADDGENE_1503 4638 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_12 complete plasmid sequence KEYWORDS . SOURCE pPD96_12 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1503 at www.addgene.org FEATURES Location/Qualifiers source 1..4638 /organism="Addgene plasmid pPD96_12" /mol_type="other DNA" misc_feature 46..71 /label="unc54.-204" misc_feature 261..284 /label="unc54.5'" misc_feature 302..343 /label="SynIVS.A" CDS 377..1288 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFSYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 377..403 /label="SV40.NLS" gene 419..1279 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 590..640 /label="SynIVS.R" misc_feature 653..676 /label="gfp.S65S" misc_feature 795..845 /label="SynIVS.S" misc_feature 1010..1060 /label="SynIVS.T" misc_feature 1374..1424 /label="SynIVS.L" promoter 2552..2580 /label="Amp_promoter" CDS 2622..3482 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2622..3482 /label="Ampicillin" /gene="Ampicillin" rep_origin 3637..4256 /label="pBR322_origin" misc_feature 4472..4498 /label="lacI.C" promoter 4565..4594 /label="lac_promoter" misc_feature 4629..4647 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCAATTC 61 TTGAGACTTC CACTATCTTC TCTGGCACCC TCCACTACGC GTAGGCGTCT CTCGCTTCGT 121 GTATTCCCGG GAAGCCGGGT TCCCGTCTCT CCCGCCGCTG CCGCTGCCGC ACACAGCTTT 181 ACACCTCGTA GAATCCCCAA AGAGGGGCGT GGCTTGCGGG TGCCAACATC CTCCTGCCGA 241 GGAAGAAGCA GGCACTCATC ACTCGCATCA TCAACCTCGG GATTGGCCAA AGGACCCAAA 301 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 361 ACCGAGCTCA GAAAAAATGA CTGCTCCAAA GAAGAAGCGT AAGGTACCGG TAGAAAAAAT 421 GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA 481 TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA 541 ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg taagtttaaa 601 catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG TCACTACTTT 661 CTCTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAT ATGAAACgGC ATGACTTTTT 721 CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA AAGATGACGG 781 GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt 841 ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG 901 TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC 961 ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg taagtttaaa 1021 catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA TTAGACACAA 1081 CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC CAATTGGCGA 1141 TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC TTTCGAAAGA 1201 TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG CTGGGATTAC 1261 ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG CGCCGGTCGC 1321 TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC AGAgtaagtt 1381 taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC GCGCCCGTGC 1441 CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT CCCTGTGCTC 1501 CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT TTTTAGCTTC 1561 TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA ATTCGTAATA 1621 AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC CCAAAATCTA 1681 CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT TTGAAACATC 1741 ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT ATGACCGCAA 1801 TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA TCGTGAAAAA 1861 GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT AATTTTCCTG 1921 CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG CGTTTTTCTT 1981 GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG GTTTGGGTTT 2041 GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT GTCTATGGGG 2101 TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT TTCCTACTAG 2161 TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 2221 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 2281 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 2341 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2401 GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA 2461 ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 2521 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 2581 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 2641 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 2701 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 2761 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 2821 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 2881 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2941 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 3001 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 3061 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 3121 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 3181 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 3241 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 3301 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 3361 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 3421 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 3481 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 3541 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 3601 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 3661 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 3721 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 3781 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 3841 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3901 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3961 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 4021 AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 4081 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 4141 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 4201 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT 4261 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC 4321 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC 4381 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC 4441 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT 4501 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC 4561 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT 4621 TTCACACAGG AAACAGCT // 1504 LOCUS ADDGENE_1504 4574 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_32 complete plasmid sequence KEYWORDS . SOURCE pPD96_32 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1504 at www.addgene.org FEATURES Location/Qualifiers source 1..4574 /organism="Addgene plasmid pPD96_32" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 134..207 /label="ATG-MitLS" CDS 134..1090 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MALLQSRLLLSAPRRAAATARASSVPVEKMSKGEELFTGVVPIL VELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNF QPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQF GTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP DNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" gene 221..1081 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 392..442 /label="SynIVS.R" misc_feature 464..483 /label="gfp.S65C" misc_feature 597..647 /label="SynIVS.S" misc_feature 812..862 /label="SynIVS.T" misc_feature 1176..1226 /label="SynIVS.L" promoter 2354..2382 /label="Amp_promoter" CDS 2424..3284 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2424..3284 /label="Ampicillin" /gene="Ampicillin" rep_origin 3439..4058 /label="pBR322_origin" misc_feature 4274..4300 /label="lacI.C" promoter 4367..4396 /label="lac_promoter" misc_feature 4431..4449 /label="M13r_primer" misc_feature 4463..4513 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGGAGGGT 121 ACCTCGAGAA AAAATGGCAC TCCTGCAATC ACGTCTCCTG CTATCCGCAC CTCGTCGTGC 181 CGCTGCAACT GCAAGAGCTT CATCCGTACC GGTAGAAAAA ATGAGTAAAG GAGAAGAACT 241 TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT 301 TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT 361 TTGCACTACT GGAAAACTAC CTGTTCCATG Ggtaagttta aacatatata tactaactaa 421 ccctgattat ttaaattttc agCCAACACT TGTCACTACT TTCTgTTATG GTGTTCAATG 481 CTTcTCgAGA TACCCAGATC ATATGAAACg GCATGACTTT TTCAAGAGTG CCATGCCCGA 541 AGGTTATGTA CAGGAAAGAA CTATATTTTT CAAAGATGAC GGGAACTACA AGACACgtaa 601 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagGTG CTGAAGTCAA 661 GTTTGAAGGT GATACCCTTG TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA 721 TGGAAACATT CTTGGACACA AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT 781 GGCAGACAAA CAAAAGAATG GAATCAAAGT Tgtaagttta aacatgattt tactaactaa 841 ctaatctgat ttaaattttc agAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT 901 TCAACTAGCA GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC 961 AGACAACCAT TACCTGTCCA CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA 1021 CCACATGGTC CTTCTTGAGT TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT 1081 ATACAAATAG CATTCGTAGA ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC 1141 TGGTGTCAAA AATAATAGGG GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa 1201 ctaacgagta atatttaaat tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA 1261 ATTACTCTTC AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT 1321 ATTATCAAAA AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC 1381 AATGAAATTG TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG 1441 TGCTCCCTCC CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA 1501 CTTCTTATGT TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC 1561 AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG 1621 TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG 1681 AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT 1741 TCCCCTATTG TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT 1801 GATGAGCACG ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG 1861 AGTAGAAGTT GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA 1921 GAATACATTC CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT 1981 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 2041 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 2101 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 2161 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 2221 GCGGCCTTAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG 2281 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT 2341 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 2401 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 2461 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG 2521 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA 2581 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC 2641 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 2701 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG 2761 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG 2821 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA 2881 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA 2941 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 3001 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 3061 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT 3121 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC 3181 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA 3241 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT 3301 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 3361 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG 3421 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA 3481 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG 3541 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG 3601 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 3661 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA 3721 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG 3781 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC 3841 GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA 3901 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 3961 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT 4021 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT 4081 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC 4141 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG 4201 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 4261 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 4321 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 4381 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT 4441 ATGACCATGA TTACGCCAAG CTgtaagttt aaacatgatc ttactaacta actattctca 4501 tttaaatttt cagAGCTTAA AAATGGCTGA AATCACTCAC AACGATGGAT ACGCTAACAA 4561 CTTGGAAATG AAAT // 1505 LOCUS ADDGENE_1505 8115 bp DNA circular SYN 01/03/2005 DEFINITION pPD96_62 complete plasmid sequence KEYWORDS . SOURCE pPD96_62 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1505 at www.addgene.org FEATURES Location/Qualifiers source 1..8115 /organism="Addgene plasmid pPD96_62" /mol_type="other DNA" misc_feature 62..103 /label="SynIVS.A" misc_feature 105..122 /label="rf22.11" CDS 141..1736 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" misc_feature 141..167 /label="SV40.NLS" gene 183..1043 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 354..404 /label="SynIVS.R" misc_feature 426..445 /label="gfp.S65C" misc_feature 559..609 /label="SynIVS.S" misc_feature 774..824 /label="SynIVS.T" gene 1140..4693 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1274..1324 /label="SynIVS.B" CDS 1775..4696 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1952..2002 /label="SynIVS.D" misc_feature 2393..2443 /label="SynIVS.E" misc_feature 2700..2750 /label="SynIVS.F" misc_feature 2981..3031 /label="SynIVS.G" misc_feature 3268..3318 /label="SynIVS.H" misc_feature 3634..3684 /label="SynIVS.I" misc_feature 3945..3995 /label="SynIVS.J" misc_feature 4351..4401 /label="SynIVS.K" misc_feature 4717..4767 /label="SynIVS.L" promoter 5895..5923 /label="Amp_promoter" CDS 5965..6825 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5965..6825 /label="Ampicillin" /gene="Ampicillin" rep_origin 6980..7599 /label="pBR322_origin" misc_feature 7815..7841 /label="lacI.C" promoter 7908..7937 /label="lac_promoter" misc_feature 7972..7990 /label="M13r_primer" misc_feature 8004..8054 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GGATCCCCGG GATTGGCCAA AGGACCCAAA 61 Ggtatgtttc gaatgatact aacataacat agaacatttt cagGAGGACC CTTGCTTGGA 121 GGGTACCGAG CTCAGAAAAA ATGACTGCTC CAAAGAAGAA GCGTAAGGTA CCGGTAGAAA 181 AAATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG 241 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG 301 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGgtaagtt 361 taaacatata tatactaact aaccctgatt atttaaattt tcagCCAACA CTTGTCACTA 421 CTTTCTgTTA TGGTGTTCAA TGCTTcTCgA GATACCCAGA TCATATGAAA CgGCATGACT 481 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG 541 ACGGGAACTA CAAGACACgt aagtttaaac agttcggtac taactaacca tacatattta 601 aattttcagG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA 661 AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA 721 ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTgtaagtt 781 taaacatgat tttactaact aactaatctg atttaaattt tcagAACTTC AAAATTAGAC 841 ACAACATTGA AGATGGAAGC GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG 901 GCGATGGCCC TGTCCTTTTA CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA 961 AAGATCCCAA CGAAAAGAGA GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA 1021 TTACACATGG CATGGATGAA CTATACAAAT gcCCGGTGGG TGAAGACCAG AAACAGCACC 1081 TCGAACTGAG CCGCGATATT GCCCAGCGTT TCAACGCGCT GTATGGCGAG ATCGATCCCG 1141 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG 1201 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC 1261 AACAGTTGCG aAGgtaagtt taaacagatc catactaact aacttgttct gacataattt 1321 tcagCtTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA 1381 GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA 1441 TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC 1501 CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA 1561 GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC 1621 TGTGGTGCAA CGGGCGCTGG GTCGGTTACG GCCAGgtaag tttaattaag ttgataCTAA 1681 CTAACAAGAT CTGATTAATT TTCagGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC 1741 ATTTTTACGC GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGCTGGA GTGACGGCAG 1801 TTATCTGGAA GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT 1861 GCATAAACCG ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT 1921 CtccCGCGCT GTACTGGAGG CTGAAGTcCA Ggtaagttta aacaggatct tactaactaa 1981 catgctaaca ctgaattttc agATGTGCGG CGAGTTGCGT GACTACCTAC GGGTAACAGT 2041 TTCTTTATGG CAGGGTGAAA CGCAGGTCGC CAGCGGCACC GCGCCTTTCG GCGGTGAAAT 2101 TATCGATGAG CGTGGTGGTT ATGCCGATCG CGTCACACTA CGTCTGAACG TCGAAAACCC 2161 GAAACTGTGG AGCGCCGAAA TCCCGAATCT CTATCGTGCG GTGGTTGAAC TGCACACCGC 2221 CGACGGCACG CTGATTGAAG CAGAAGCCTG CGATGTCGGT TTCCGCGAGG TGCGGATTGA 2281 AAATGGTCTG CTGCTGCTGA ACGGCAAGCC GTTGCTGATT CGAGGCGTTA ACCGTCACGA 2341 GCATCATCCT CTGCATGGTC AGGTCATGGA TGAGCAGACG ATGGTGCAGG ATgtaagttt 2401 aaactattcg ttactaacta actttaaaca tttaaatttt cagATCCTGC TGATGAAGCA 2461 GAACAACTTT AACGCCGTGC GCTGTTCGCA TTATCCGAAC CATCCGCTGT GGTACACGCT 2521 GTGCGACCGC TACGGCCTGT ATGTGGTGGA TGAAGCCAAT ATTGAAACCC ACGGCATGGT 2581 GCCAATGAAT CGTCTGACCG ATGATCCGCG CTGGCTACCG GCGATGAGCG AACGCGTAAC 2641 GCGAATGGTG CAGCGCGATC GTAATCACCC GAGTGTGATC ATCTGGTCGC TGGGGAATGg 2701 taagtttaaa cagttgaata ctaactaacg gagatctttg aaattttcag AATCAGGCCA 2761 CGGCGCTAAT CACGACGCGC TGTATCGCTG GATCAAATCT GTCGATCCTT CCCGCCCGGT 2821 GCAGTATGAA GGCGGCGGAG CCGACACCAC GGCCACCGAT ATTATTTGCC CGATGTACGC 2881 GCGCGTGGAT GAAGACCAGC CCTTCCCGGC TGTGCCGAAA TGGTCCATCA AAAAATGGCT 2941 TTCGCTACCT GGAGAGACGC GCCCGCTGAT tCTTTGCGAG gtaagtttaa acagaactct 3001 actaactaac acattagatc ctaattttca gTACGCtCAC GCGATGGGcA ACAGTCTTGG 3061 CGGTTTCGCT AAATACTGGC AGGCGTTTCG TCAGTATCCC CGTTTACAGG GCGGCTTCGT 3121 CTGGGACTGG GTGGATCAGT CGCTGATTAA ATATGATGAA AACGGCAACC CGTGGTCGGC 3181 TTACGGCGGT GATTTTGGCG ATACGCCGAA CGATCGCCAG TTCTGTATGA ACGGTCTGGT 3241 CTTTGCCGAC CGCACGCCGC ATCCAGCgta agtttaaaca ataacctact aactaacgta 3301 gataatttaa attttcagGC TGACGGAAGC AAAACACCAG CAGCAGTTTT TCCAGTTCCG 3361 TTTATCCGGG CAAACCATCG AAGTGACCAG CGAATACCTG TTCCGTCATA GCGATAACGA 3421 GCTCCTGCAC TGGATGGTGG CGCTGGATGG TAAGCCGCTG GCAAGCGGTG AAGTGCCTCT 3481 GGATGTCGCT CCACAAGGTA AACAGTTGAT TGAACTGCCT GAACTACCGC AGCCGGAGAG 3541 CGCCGGGCAA CTCTGGCTCA CAGTACGCGT AGTGCAACCG AACGCGACCG CATGGTCAGA 3601 AGCCGGGCAC ATCAGCGCaT GGCAGCAGTG GAGgtaagtt taaacaagat cctactaact 3661 aactctacat tgatgaattt tcagACTGGC GGAAAACCTC AGTGTGACGC TCCCCGCCGC 3721 GTCCCACGCC ATCCCGCATC TGACCACCAG CGAAATGGAT TTTTGCATCG AGCTGGGTAA 3781 TAAGCGTTGG CAATTTAACC GCCAGTCAGG CTTTCTTTCA CAGATGTGGA TTGGCGATAA 3841 AAAACAACTG CTGACGCCGC TGCGCGATCA GTTCACCCGT GCACCGCTGG ATAACGACAT 3901 TGGCGTAAGT GAAGCGACCC GCATTGACCC TAACGCCTGG GTCGgtaagt ttaaacaaag 3961 ttgtactaac taacgaagat cttgataatt ttcagAACGC TGGAAGGCGG CGGGCCATTA 4021 CCAGGCCGAA GCAGCGTTGT TGCAGTGCAC GGCAGATACA CTTGCTGATG CGGTGCTGAT 4081 TACGACCGCT CACGCGTGGC AGCATCAGGG GAAAACCTTA TTTATCAGCC GGAAAACCTA 4141 CCGGATTGAT GGTAGTGGTC AAATGGCGAT TACCGTTGAT GTTGAAGTGG CGAGCGATAC 4201 ACCGCATCCG GCGCGGATTG GCCTGAACTG CCAGCTGGCG CAGGTAGCAG AGCGGGTAAA 4261 CTGGCTCGGA TTAGGGCCGC AAGAAAACTA TCCCGACCGC CTTACTGCCG CCTGTTTTGA 4321 CCGCTGGGAT CTGCCATTGT CAGACATGTA gtaagtttaa acttgatagt actaactaac 4381 atgtttcatt taaattttca gTACCCCGTA CGTCTTCCCG AGCGAAAACG GTCTGCGCTG 4441 CGGGACGCGC GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC AGTTCAACAT 4501 CAGCCGCTAC AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC TGCACGCGGA 4561 AGAAGGCACA TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG ACGACTCCTG 4621 GAGCCCGTCA GTATCGGCGG AATTCCAACT GAGCGCCGGT CGCTACCATT ACCAACTTGT 4681 CTGGTGTCAA AAATAATAGG GGCCGCTGTC ATCAGAgtaa gtttaaactg agttctacta 4741 actaacgagt aatatttaaa ttttcagCAT CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC 4801 AATTACTCTT CAACATCCCT ACATGCTCTT TCTCCCTGTG CTCCCACCCC CTATTTTTGT 4861 TATTATCAAA AAACTTCTCT TAATTTCTTT GTTTTTTAGC TTCTTTTAAG TCACCTCTAA 4921 CAATGAAATT GTGTAGATTC AAAAATAGAA TTAATTCGTA ATAAAAAGTC GAAAAAAATT 4981 GTGCTCCCTC CCCCCATTAA TAATAATTCT ATCCCAAAAT CTACACAATG TTCTGTGTAC 5041 ACTTCTTATG TTTTTTACTT CTGATAAATT TTTTTGAAAC ATCATAGAAA AAACCGCACA 5101 CAAAATACCT TATCATATGT TACGTTTCAG TTTATGACCG CAATTTTTAT TTCTTCGCAC 5161 GTCTGGGCCT CTCATGACGT CAAATCATGC TCATCGTGAA AAAGTTTTGG AGTATTTTTG 5221 GAATTTTTCA ATCAAGTGAA AGTTTATGAA ATTAATTTTC CTGCTTTTGC TTTTTGGGGT 5281 TTCCCCTATT GTTTGTCAAG ATTTCGAGGA CGGCGTTTTT CTTGCTAAAA TCACAAGTAT 5341 TGATGAGCAC GATGCAAGAA AGATCGGAAG AAGGTTTGGG TTTGAGGCTC AGTGGAAGGT 5401 GAGTAGAAGT TGATAATTTG AAAGTGGAGT AGTGTCTATG GGGTTTTTGC CTTAAATGAC 5461 AGAATACATT CCCAATATAC CAAACATAAC TGTTTCCTAC TAGTCGGCCG TACGGGCCCT 5521 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 5581 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 5641 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 5701 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 5761 GGCGGCCTTA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG 5821 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 5881 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 5941 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 6001 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 6061 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 6121 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 6181 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 6241 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 6301 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 6361 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 6421 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 6481 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 6541 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 6601 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 6661 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 6721 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 6781 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 6841 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 6901 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 6961 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 7021 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 7081 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 7141 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 7201 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 7261 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 7321 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 7381 CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 7441 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 7501 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 7561 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 7621 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 7681 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 7741 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 7801 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 7861 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 7921 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 7981 TATGACCATG ATTACGCCAA GCTgtaagtt taaacatgat cttactaact aactattctc 8041 atttaaattt tcagAGCTTA AAAATGGCTG AAATCACTCA CAACGATGGA TACGCTAACA 8101 ACTTGGAAAT GAAAT // 1506 LOCUS ADDGENE_1506 4970 bp DNA circular SYN 01/03/2005 DEFINITION pPD52_37 complete plasmid sequence KEYWORDS . SOURCE pPD52_37 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1506 at www.addgene.org FEATURES Location/Qualifiers source 1..4970 /organism="Addgene plasmid pPD52_37" /mol_type="other DNA" misc_feature 1639..1680 /label="SynIVS.A" misc_feature 1687..1704 /label="rf21.28" promoter 2884..2912 /label="Amp_promoter" CDS 2954..3814 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2954..3814 /label="Ampicillin" /gene="Ampicillin" rep_origin 3969..4588 /label="pBR322_origin" misc_feature 4804..4830 /label="lacI.C" promoter 4897..4926 /label="lac_promoter" misc_feature 4961..4979 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGgaatt cCCCGGATCC AGATTCGAAC 61 CAGCAATGAC TCTACTGAAG AGCGCGCGCT TTACCACTTC TCCAAACAGG CGAGTGGCGG 121 CCGCGGTTCC AAGGTATATG TGAAACCAAC CGAGCTGTAC GAAGTAGGAA GCTTCCATTA 181 CTTTTTCAGA TATTTAGTTC TGAAAACTTT GTTAACTCTC CTTATCAACA ACTAAATGGC 241 CCTTTTTGTA GTTATTTCTA CTGGAAAAAA ATGTGCCTGT AAATAATATc CTGTTTATAA 301 ACGTTGTTGT cCAGTGAAAA CATTCCCTTG ACCTGAAATT TATCGATAAA AGATAAATTT 361 ATCTTGAAAA CTACtTTTTC GAGTGTATTT TTAAACATTC TACAAATTCC ACTTAATAGC 421 AGGTCGGGAA CTTCGGCGAT TCCTCGAATA AGCTATTTTC AAAAAAAAAA TTAGGCCCAA 481 AATTTGTTTC TGAAAAACGA CCCCAATCTT GTATTTTCCA GTAATAAATA CAATCTGAAT 541 TTTTTTCGAA ATTATTTTTC GCATCGAAAT TGGAAAACGT AGTAAAAAAT ACGGCCGTAG 601 TAAAAGAGTA AACCGTAGTA AAAGAGcaac gtagtaaaag atgaggtagt aaaagagCAT 661 TTGCCTAATT TCTTTTATTT TTCACATTAT ATGTCCAGTT AAAAAAAAAG AACTCTGAGA 721 AATAAATTTT GAAAATCAGG TAAAAAAAGT TAGATGTTTC AGACGTCACC TTGTTGTTaA 781 TATgGTCTCT CATATGATTg GTGACATCAG Aatttttaac ttggaattga ACATTTtaAT 841 TCTTGATTTA TTATTTTCAA GATTTAATTT TAAGTTTTAA AAATAGATTT TTTTTTCAAA 901 AAAAAAACAA GGTTGTAAAA ATTGTTAAAA CTGGAAACAT CTTTTAGCTA TTTTTATTTT 961 AGCCTGCAAG AATTGCTCCT GTGGAGAAAC GTGTAAGTGT ACCGTCGAAA AATGTTGCTG 1021 CGAGGAAGAG AAAAAATCGT GCTGTGAAGG AAAAACCGAG AAGACTGAGC AAAAGAAATA 1081 ACCAATGAGC AAGTGAACTT TTCCCACGTC TACTACTAAA TTATTGTCGA TCTATAATTC 1141 TTTCGCTTAT CAATCTTGTC AATTGAAATA AAACAATATT TTTCTAATTC TTTTTGGAAC 1201 GAACACACGT GTTTAAATGA ATGTTGTGCT AAAAACGTCA CATCAATGGT ACGTGAATGT 1261 TGCAAACACC TTGTCAATAA CTGATAAAAT CAGAAACTAG AGCTGTGACT GAATCGTATA 1321 CTAGAACGGA GTCTCTCTAA AAACGTTCTA AAAACAAACA AAAAATTGTG CAAAGGATTA 1381 GAGTGCTCAA GATCAATGAG CAAACTCACA ATCAACTATC TGTTATTGTT TTGGGTCTCT 1441 TCTATATCTC TATTCTTTTA GTATCATAAG TTTGTACATT GTGACAGGGC CACCCTCTTT 1501 TATCACATAT TTGAAGTGGT AAACAGCCAG CAAAAACCAA ATAAAAAGGC AGTGAGAAAA 1561 AGAAGAAGGC AGCTCAATTT GACTGCTGAA ATTAAGAAct ctCTAGAGGA TCCCCGGGAT 1621 TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG 1681 GAGGACCCTT GGCTAGCGTC GACGGTACCA TGGTATTGAT ATCTGAGCTC CGCATCGGCC 1741 GCTGTCATCA GATCGCCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC 1801 AACATCCCTA CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA 1861 AACTTCTCTT AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG 1921 TGTAGATTCA AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC 1981 CCCCATTAAT AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT 2041 TTTTTACTTC TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT 2101 ATCATATGTT ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC 2161 TCATGACGTC AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA 2221 TCAAGTGAAA GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG 2281 TTTGTCAAGA TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG 2341 ATGCAAGAAA GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT 2401 GATAATTTGA AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC 2461 CCAATATACC AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG 2521 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT 2581 TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC 2641 GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT 2701 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA 2761 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC 2821 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 2881 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 2941 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC 3001 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA 3061 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA 3121 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG 3181 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC 3241 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC 3301 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT 3361 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA 3421 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 3481 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT 3541 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG 3601 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG 3661 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT 3721 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 3781 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT 3841 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT 3901 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC 3961 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 4021 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 4081 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT 4141 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 4201 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 4261 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 4321 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG 4381 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 4441 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 4501 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 4561 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4621 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC 4681 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG 4741 CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA 4801 TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT 4861 TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG 4921 TATGTTGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1507 LOCUS ADDGENE_1507 5273 bp DNA circular SYN 01/03/2005 DEFINITION pPD54_01 complete plasmid sequence KEYWORDS . SOURCE pPD54_01 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1507 at www.addgene.org FEATURES Location/Qualifiers source 1..5273 /organism="Addgene plasmid pPD54_01" /mol_type="other DNA" misc_feature 1942..1983 /label="SynIVS.A" misc_feature 1990..2007 /label="rf21.28" promoter 3187..3215 /label="Amp_promoter" gene 3257..4117 /label="Ampicillin" /gene="Ampicillin" CDS 3257..4117 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4272..4891 /label="pBR322_origin" misc_feature 5107..5133 /label="lacI.C" promoter 5200..5229 /label="lac_promoter" misc_feature 5264..5282 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGaATT CGTTATTTTG TGTTACTATC 61 TATACGTGAC TACATATAAA ATATTCATTT GTTCTTCTTT TATAAAAGTA AAAAGGTATT 121 TTTTGAGCTC TCAAATTCTT TAATTGATTA TACAACTTTC AAAAATTAAA GCAGGAATCA 181 CATTATAATT TTTGGGATTC CCCGATATCT AAATGGTTGT TTTGCGGATA TTGACTTTCC 241 TTTCAGATGA CTCGGTGGCA CTGCTTTTAC AAATCGATTT TTCTGGAAAA TTTTGAATTA 301 TTTAGCCTAT AAAATCCACA AAACTTACAT CATCCGGATG GCACCTCCTG AATTTAAGAC 361 CATAGTTGAT AGTAGGTGGA GGAATTGTTG GATAACGTGG GTCTTGATGT ACTCTGAAAA 421 ATTTTAAATG GTTGTGTAAT GAGTGTCAGA GCTCACCGAA TTTTTCTGGA GTGATGTCTT 481 GGACGATATG CAATGTTTCT GGGTTTTTGA TGGTGCTTTA TACTATTAAA GTTTCTTAAT 541 ACAGTAACAT CCTCGTTATT TTGAGTATTT CGAGAAGTTG AAGTATTTCT TTGCTTTCTC 601 ATTTTTCTTC TAACTATTCG CTTTGGCTGT TTCTTCACTT TTCTTTTCTT CTTATGTGTT 661 TCCGTTGTGT AAATTGGACC AGGTAGAATA ATCGGTGATT CAACTTCCCG AATAGTCATC 721 ACTTCGTAAT TCATCATTCT ATCGTCATCT TGTTCACGAG AATGATTGTC AAATAATCTT 781 GGCTGCTCTG TAATAACATC TTCCTGAAGA CGAGCTTCAC TAAAGCGCCT TCTGACAGAA 841 TCAGGGCTTG TTGGCACAGA GTTGAATGGG GTCCAGTCGT CTGAAATTTT AAAATTGAAT 901 CACTTTTACG gGTAAGGCAg GATATCACGA TAGTGTCTAA AAGGAGGAAG AATTTTTTCT 961 TGTTTTCTGC CTGGAATTGT TTCAGGATTA AAATAGCAAG CCTCGAAGTT TgCCCGCTGG 1021 CCCGAAATAG CCTATTCATC AGTCAGCCGC AGAAGGGTTG TTTTTGGGGA CCCCGCTTTA 1081 CCGGTGTAGT ATTATTTGAC ATTGGTAATT TTTTAATTAC GGTTAAACAT AAGTTAAACA 1141 ATAAAATTAC AAACCTGCAC AAAGAGTTCC TGGGCCACAT AATGTTTCAT CAATATCACT 1201 TCCCCAGGCG CTATGTCTAT CTTCTGAAAT CTCAGTTTTC TTTTCTAAAT TAAATAATAT 1261 TTCAAACCTT CTACATGTTC TGGAGCGGTT CTGGAACTTT TTTGTCCCAT AGAGAATACA 1321 AAAAGAGACG AAAATGGTTC AGTGGAACGA AACAAACGTG GGATGTAACC ATGGAAGTGA 1381 GAATAATTGA TGGAATAGCT AATGAGCTGA AGCTGAGTAG ATCAGAGACT ACTTTATTCT 1441 AAAAAGTACA GTAGTTGGAA AATTACATGT TTCATTTCTA ATTTTCAAGG AAAAGCTAGT 1501 AATTACCGTA ATCTTGTTTG TACCTGAAAT TTTATGTACT GCAGGTGACC AAGTATGTTT 1561 GAGGCATGAC TTCACACACC TAACTGATAA AGGCCTCTAT CACAAACTAG AGTTGTGACG 1621 GAAAATTCAA CTTCTCAGaA TATAGCTCAA AATCTATCAA ATTTTATTTT CAAAAATCCA 1681 AATAATTGTG CACGCAATGT ACTTACTGCT TCATAAAGTT CAGAAGAATT GGATAAATTT 1741 GAATGAAGTT TTCAAAGCTT TTATCAGTGA CTGTACATTG TGATAGGCTT GTGCTGTTAT 1801 CAGCTGCCTC AAATAGGTTG TCGCTTGAAA ATTTATATAA AAGGCCTACC AGCAGACATG 1861 AGAATCAAGC TTCAAAGGCT CTACTCAAAA ATaGTCTGCA AGTGTCTAGA GGATCCCCGG 1921 GATTGGCCAA AGGACCCAAA GGTATGTTTC GAATGATACT AACATAACAT AGAACATTTT 1981 CAGGAGGACC CTTGGCTAGC GTCGACGGTA CCATGGTATT GATATCTGAG CTCCGCATCG 2041 GCCGCTGTCA TCAGATCGCC ATCTCGCGCC CGTGCCTCTG ACTTCTAAGT CCAATTACTC 2101 TTCAACATCC CTACATGCTC TTTCTCCCTG TGCTCCCACC CCCTATTTTT GTTATTATCA 2161 AAAAACTTCT CTTAATTTCT TTGTTTTTTA GCTTCTTTTA AGTCACCTCT AACAATGAAA 2221 TTGTGTAGAT TCAAAAATAG AATTAATTCG TAATAAAAAG TCGAAAAAAA TTGTGCTCCC 2281 TCCCCCCATT AATAATAATT CTATCCCAAA ATCTACACAA TGTTCTGTGT ACACTTCTTA 2341 TGTTTTTTAC TTCTGATAAA TTTTTTTGAA ACATCATAGA AAAAACCGCA CACAAAATAC 2401 CTTATCATAT GTTACGTTTC AGTTTATGAC CGCAATTTTT ATTTCTTCGC ACGTCTGGGC 2461 CTCTCATGAC GTCAAATCAT GCTCATCGTG AAAAAGTTTT GGAGTATTTT TGGAATTTTT 2521 CAATCAAGTG AAAGTTTATG AAATTAATTT TCCTGCTTTT GCTTTTTGGG GTTTCCCCTA 2581 TTGTTTGTCA AGATTTCGAG GACGGCGTTT TTCTTGCTAA AATCACAAGT ATTGATGAGC 2641 ACGATGCAAG AAAGATCGGA AGAAGGTTTG GGTTTGAGGC TCAGTGGAAG GTGAGTAGAA 2701 GTTGATAATT TGAAAGTGGA GTAGTGTCTA TGGGGTTTTT GCCTTAAATG ACAGAATACA 2761 TTCCCAATAT ACCAAACATA ACTGTTTCCT ACTAGTCGGC CGTACGGGCC CTTTCGTCTC 2821 GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA 2881 GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT 2941 GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC 3001 CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGGCCT 3061 TAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA 3121 GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 3181 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 3241 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 3301 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 3361 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT 3421 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG 3481 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 3541 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 3601 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 3661 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA 3721 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 3781 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 3841 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 3901 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 3961 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG 4021 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 4081 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA 4141 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT 4201 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 4261 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 4321 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 4381 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT 4441 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 4501 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 4561 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 4621 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA 4681 TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4741 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4801 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4861 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4921 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC 4981 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT 5041 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT 5101 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC 5161 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC 5221 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCT // 1508 LOCUS ADDGENE_1508 2997 bp DNA circular SYN 01/03/2005 DEFINITION pPD61_125 complete plasmid sequence KEYWORDS . SOURCE pPD61_125 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1508 at www.addgene.org FEATURES Location/Qualifiers source 1..2997 /organism="Addgene plasmid pPD61_125" /mol_type="other DNA" promoter complement(152..170) /label="T7_promoter" misc_feature 190..338 /label="lacZ_a" misc_feature complement(181..197) /label="M13f20_primer" rep_origin 356..662 /label="f1_origin" promoter 855..883 /label="Amp_promoter" gene 925..1785 /label="Ampicillin" /gene="Ampicillin" CDS 925..1785 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 1940..2559 /label="pBR322_origin" misc_feature 2775..2801 /label="lacI.C" promoter 2868..2897 /label="lac_promoter" misc_feature 2932..2950 /label="M13r_primer" promoter 2967..2986 /label="T3_promoter" ORIGIN 1 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTGGAT CCACCGGTTC CATGGCTAGC 61 CACGTGACGC GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC 121 GACCTCGAGG GGGGGCCCGG TACCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC 181 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG 241 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG 301 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT 361 AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC 421 GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 481 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 541 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT 601 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC 661 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 721 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 781 AACGCTTACA ATTTAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 841 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 901 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC 961 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 1021 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT 1081 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT 1141 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC 1201 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG 1261 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 1321 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC 1381 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA 1441 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA 1501 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT 1561 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 1621 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG 1681 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT 1741 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT 1801 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG 1861 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA 1921 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA 1981 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 2041 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT 2101 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA 2161 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT 2221 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG 2281 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 2341 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA 2401 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT 2461 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG 2521 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC 2581 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 2641 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC 2701 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT 2761 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG 2821 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG 2881 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC 2941 TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTG // 1509 1510 LOCUS ADDGENE_1510 10549 bp DNA circular SYN 01/03/2005 DEFINITION BK48 complete plasmid sequence KEYWORDS . SOURCE BK48 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1510 at www.addgene.org FEATURES Location/Qualifiers source 1..10549 /organism="Addgene plasmid BK48" /mol_type="other DNA" misc_feature 28..52 /label="let858.-562" misc_feature 569..592 /label="let858.Tsa" misc_feature 596..619 /label="let858.N" gene 1177..2037 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" CDS 1177..2406 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPVRSRSPRRSSARSPSRSPRRRRERSSERKQSEEPAPLPEKKKK EPLDILRTRTGGAYIPPAKLRLMQQQISDKQSEQYQRMNWERMKKKIHGLVNRVNAKN LVQIVRELLQENVIRSK" misc_feature 1348..1398 /label="SynIVS.R" misc_feature 1420..1439 /label="gfp.S65C" misc_feature 1553..1603 /label="SynIVS.S" misc_feature 1768..1818 /label="SynIVS.T" CDS 6198..6902 /label="Orf2" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 6878..6902 /label="let858.C" misc_feature 6980..7009 /label="let858.3'" promoter complement(7734..7752) /label="T7_promoter" misc_feature 7757..7917 /label="lacZ_a" misc_feature complement(7760..7776) /label="M13f20_primer" rep_origin 7935..8241 /label="f1_origin" promoter 8435..8463 /label="Amp_promoter" gene 8505..9365 /label="Ampicillin" /gene="Ampicillin" CDS 8505..9365 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 9520..10139 /label="pBR322_origin" misc_feature 10355..10381 /label="lacI.C" misc_feature 10512..10530 /label="M13r_primer" promoter 10547..10566 /label="T3_promoter" ORIGIN 1 TAACCCTCAC TAAAGGGAAC AAAAGCTGGc tccaaaatcg tcttccgctc tgaaaaacga 61 aagtggacct ttgacatccg aaaaaatggg cgaaaaaatg aaattgagct ttttgggtcg 121 aaaaaaatgt ttttagaatg ctgagaacac gttaaacacg aagatcatat ttattttgag 181 acccggatgc tctgaaaatg tctgacatag atttaaaaaa gcatatatat atttttcatt 241 ttcaacgtga aagttttgtg caactttata gaatctccta ttggcacatt gttttttatt 301 taactgaggc agtttttgaa cacctttttg aaactttgaa tctctttgaa gtatactgtc 361 gaaaagactg acttgagcgt tcgaaatgcc agaagaaaac tatatttgaa tctcgcgcta 421 aattgagaaa tgcaaccgcg ctccactgga caattggaaa aaaaatttat tcggaggcga 481 caacggtatt ttcgaaattg attttctgtg tattttctca ttttttataa attcttcttt 541 gatttatcgt tcgtttgtga gaaatttaat tgtattcaaa cttttttata GTAAGATGTC 601 GTCGAGTCGG TCACAATCAC CTGAAACTCC AAAGGCAGCC AGTGAGGAAC GTGAAGAAGA 661 AGAAAAAGAG TCATCTGAAC AGgtttgatt ttctttctgg tcaaaaagat gaaattattg 721 attttcagCC AGATACTCCC AAAACTAGCA GCGAGAAGTC TGCAAGTCGT TCACAGTCGC 781 CCAGAGAATC GCGGGAAGTG AGCCAAGAGg tatgtttttc aaaaatcaat aactgatcat 841 aatttttatt gtttggtgaa tttaagaaaa taatattcga aaattcctct gaattatcaa 901 gattgcagta ttaatttcga gaaaaattga gatattcata gagctattgt aaattttctt 961 gatttcagAC TGAAACTTCG GAAAATCAAG AGAAAATCAA AGAAAAGGAT GACGGGGATG 1021 ATCAGCCTGG CACACCGAAC AGCTATAGAA GCCGGGAAAC TTCACCAGCT CCAAAAAGGT 1081 CCAAGGAGAC CAGgtttgtc aaaagcttcc tgcgattaat tctcatttca atttttcagA 1141 GAATCAGAGT CTCCTGAAAA ATCACCGGTA GAAAAAATGA GTAAAGGAGA AGAACTTTTC 1201 ACTGGAGTTG TCCCAATTCT TGTTGAATTA GATGGTGATG TTAATGGGCA CAAATTTTCT 1261 GTCAGTGGAG AGGGTGAAGG TGATGCAACA TACGGAAAAC TTACCCTTAA ATTTATTTGC 1321 ACTACTGGAA AACTACCTGT TCCATGGgta agtttaaaca tatatatact aactaaccct 1381 gattatttaa attttcagCC AACACTTGTC ACTACTTTCT gTTATGGTGT TCAATGCTTc 1441 TCgAGATACC CAGATCATAT GAAACgGCAT GACTTTTTCA AGAGTGCCAT GCCCGAAGGT 1501 TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA ACTACAAGAC ACgtaagttt 1561 aaacagttcg gtactaacta accatacata tttaaatttt cagGTGCTGA AGTCAAGTTT 1621 GAAGGTGATA CCCTTGTTAA TAGAATCGAG TTAAAAGGTA TTGATTTTAA AGAAGATGGA 1681 AACATTCTTG GACACAAATT GGAATACAAC TATAACTCAC ACAATGTATA CATCATGGCA 1741 GACAAACAAA AGAATGGAAT CAAAGTTgta agtttaaaca tgattttact aactaactaa 1801 tctgatttaa attttcagAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA 1861 CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC 1921 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC 1981 ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA TGAACTATAC 2041 AAATgcCCGG TTCGTTCAAG ATCTCCCAGA AGGTCTTCAG CACGTTCCCC GTCACGATCT 2101 CCTAGACGGC GCCGAGAAAG AAGCTCAGAA AGAAAGCAAT CCGAAGAGCC AGCACCGCTA 2161 CCAGAGAAAA AGAAGAAAGA GCCGCTGGAT ATTCTACGAA CAAGAACCGG AGGAGCATAT 2221 ATTCCACCCG CCAAACTTCG ACTTATGCAA CAACAGATTA GTGATAAGCA AAGTGAACAG 2281 TATCAGAGAA TGAATTGGGA AAGAATGAAG AAAAAGATTC ACGGATTGGT TAACAGAGTC 2341 AACGCGAAGA ATCTTGTTCA AATTGTCAGA GAACTTCTTC AAGAGAATGT GATTCGTTCA 2401 AAgtgagtga gaaaatcgaa ggaaaaggaa agaattaatt taatttttca gGGGACTTCT 2461 CTGCCGTGAC ATTATTCAAG CTCAGGCTTT CTCACCAGGA TTCTCTAACG TCTATGCAGC 2521 TTTGGCGGCA GTTATCAACT CGAAATTCCC TCATGTCGGT GAACTTCTTC TCCGTCGTCT 2581 GATTGTACAG TTCAAAAGAA GTTTCCGTAG AAATGACAGA GGCGTCACGG TGAACGTGAT 2641 CAAATTCATC GCACATTTGA TTAATCAACA AGTTGCTCAC GAAGTTCTTG CGCTGGAAAT 2701 CATGATTCTG ATGCTTGAAG AACCAACTGA TGATTCAGTT GAAGTCGCCA TTGCGTTCCT 2761 GAAAGAGTGT GGAGCAAAGC TTCTGGAGAT TGCTCCAGCA GCTCTTAACA GTGTCTACGA 2821 CCGTCTTCGT GCAATTCTCA TGGAAACTGA AAGATCGGAA AATGCACTGG ATCGACGTAT 2881 TCAGTATATG ATTGAGACTG CAATGCAGAT TCGAAAGGAC AAATTTGCGg taaggtagaa 2941 tatataaata gtttattaga aaaaaataaa ttagaataat ttaaattcct actagccaat 3001 caggcgacct ttttgcgcat agttctatta ttgaaaaatt tggagaattt ctcatattct 3061 cgctcggaaa tctggaattc gacgagatct tctggcttct gtgcagctgc atcgctttgt 3121 gctccctttc tcgcttgtct tctgtgtaca ccaagaacct tgttgagttc atcaactgaa 3181 tctgtgactg gcttgttgct cactggatgc actagacgac tgattctcga gaaatcagat 3241 tgagttgcga ttagggtgac ctagaaattg ggaataatac gaacttttga aaatattcag 3301 gaggattaaa aaaattattc tcgacaatcc tacaaattta cttattgcac catgttgctc 3361 caacattttt cattaaaagt taatgaaaaa atgtagaaaa tcggaaattg gcaattttca 3421 gaccattttt aagcattttc aaaaaaaaat tgcagctgaa ataaatgtca ttttcagata 3481 aatcgagcga ttttctgttg tctgacacta gtttttagtt ttaaaaaatg ttggaagaac 3541 atggtgcaat aggtaatttc atagaatttc catgtgtttt ttttcaatta accaattatc 3601 caaatcttcc aaactcacat tttgcggagc tgggctatca agaatctgct gcagttttat 3661 aagacgagca tctctgatat cactgaaaat taatttttaa tcaaaacttg aatatcaact 3721 aaacccactt attaactttc tcgatcttct gtcgttcggt acgatgacgg tgaagaagcc 3781 aattgtagta gttgatttgg ttcaagtcct ttcggtgttg tacgtcagtg tcctgcaatg 3841 ctatttagtt ataacttagg cctaagattc aatttaatga agtgattaaa tttgttctct 3901 gaacctctta agatgatctt ttggattaga aacatataag acaggtttac ctatctatta 3961 aaaaacagat caaaatagat acgaccaaat cggataatcc atgcctacct ggcatctagg 4021 aacgtgttct tagaagattt cttacgtaat cgtatgaaga aataacaatt tgatcgttgg 4081 ccagcaaaaa tagggtttta agtgggatag tgtttttatt agctaaccgg aaaattttat 4141 agtttttttt tgcaagaaac cactgaaaac cccctaattg tatacatttt ttggagcagc 4201 ttctggtctt tttgagcaat aaaattcgat aaaacagaat ttaagtgtaa attgttcaca 4261 tttagtttct attttatcaa attttgttgc tcaaaaacat tcgaagctgc tctaaaaaaa 4321 tgcattaaaa aaggggtttt cagtggtttt tcacattaaa aaagctaatt ttaactaaaa 4381 atccatcata tttccaactt tgtcacaaca ataaaatgct ggtcaaaatg tgttcgaaaa 4441 aatgtttttt tttttaattt ttataattta aaaatagttt tctttcgctg ggacacatac 4501 atttttgggc gtaaattttc agttcaaatt tccattttta caaccataat cataaagcta 4561 cgtctgatct ctctcgcact tacctgcgcc tgattcgaaa gaacaaccgt agccaaaaga 4621 acaagaagaa caagcacgta gttgtggtag tggacgttca tcacgcaata ctgaccaatg 4681 gtcgtggggt ctcactttcc gtactattga gagaggggag actgaagatg gcaattgagg 4741 acagtgtctt cgacgcacgc atgcatccat aagcataatc caggagggat ggagagaaaa 4801 atcttgtttc taagcccctc cctttgtaat acatacacat atctaatacc gaagaatggc 4861 taattgaatg gacgtcagct gttgctgtag ttgccaaggc atcatcgatg aaataactga 4921 aagaaagaat taaataatta ttgcagGCGT ATCCGGCGGT CATTGAAGAC TTGGACTTGA 4981 TTGAGGAGGA GGATCAGATC ATCCATACAC TTAATTTGGA GGATGCGGTT GATCCGGAAA 5041 ATGGGCTTAg taagtgactg accacacgcg gggggcatta atttaataaa ttgaattcca 5101 tttcagATGT GTTCAAACTA GATCCAGAAT TCGAAAAGAA CGAGGAGGTT TATGAGGAGA 5161 TCCGTAAGGA AATCATTGGA AACGCCGATA TTTCGGATGA GGATGGTGGC GACGAGTTGG 5221 ATGATGAAGA AGAGGGTAGT GATGTGGAAG AGGCTCCGAA GAAGACTACA GAGATTATTG 5281 ATAATACTGA TCAGAATTGA CTGCTTTCAG AAGgtattca ttttgagttt tgggccggca 5341 aatctgtaag ttgccggttg ccgaaaattt gctgaatttg ccggaaaaaa aaattccgga 5401 atttatttaa aaactttttg taaaaattaa attaaatttg caacttttca gAGAAGTCTA 5461 CCTGACAATG CAATCATCTT TGGACTACCA AGAAGCTGCT CACAAATTGC TGAAAATGAA 5521 GATTCCAGAC AGCATGCAGg tcagcgatgt tgcaaagaaa aattttcgac caaaaaaacc 5581 aaccaatcat aaaatttaaa aaaaaactcc gtttttttct ttttttttat acgagaaaaa 5641 ccaaaaaaat gtatttttgc caaattctaa aatactatcc ccgaaatttt caatattttc 5701 tctttcagAA CGAACTCTGC GCGATGCTTG TCGATTGTTG TGCTCAACAG CGTACCTACG 5761 AGCGATTCTA CGGAATGCTC ATCGAACGTT TCTGCCGACT TCGCCTCGAA TACCAGCAAT 5821 ACTTTGAAAA GCTCTGCCAG GACACGTATT CCACGATTCA CCGAATTGAC ATCACAAAAC 5881 TGCGGAATTT GGCTCGCCTT ATTGCTCATT TGCTCTCGAC GGATGCTATT GACTGGAAGA 5941 TTTTGGCCGA TATGAAAATG ACCGAAGAGG ACACAACTTC TTCTGGCAGA ATCTATATTA 6001 AATATATATT TAATGAACTT GTGGAGGCGA TGGGAATGGT TAAACTTCAT TCGAGAGTTA 6061 CTGATCCgtg agtttcctag agagagttgt tttcgtattc aattttccct attttcagAA 6121 CTTTGGCTCA TTGCTTTGTT GGATTATTCC CACGAACTAA TCCGAACAGC GCACGATTTT 6181 CGATCAACTT CTTCACAATG ATTGGATTGG GTGGTTTGAC GTTGGAACTT CGTGAATGGC 6241 TGGCAAAGGG TCTCAAGAAG AAGAAGGGAA TGCTGGATCA GTTGAAGGCC GAATCAAGCT 6301 CAGATTCATC GTCGTCTTCG GATTCGTCAG ACTCGTCTGA TTCTTCGGAT TCTGACGATT 6361 CATCCGACTC GTCTTCAGAT TCCTCATCTT CTTCAGAATC AGAGCCAGAA CCACCGAAGA 6421 AAAAGAAGAA GAAGAACAGT GAAGAGAGTT CCAAAAAGAA GGAAAAAGAG AATATTGGTC 6481 GACGGGATCG TGGAGACAAG AGAGCTGAAC GTCATCGTGA TCAAAGTGTG GAGAACAAGG 6541 ACAAGGATCG TCGACGTCGC CAGGATTCTG ACGAAAATCG TCGGCCAGAA CGAGGAGATG 6601 ACCGCAAGGA TCGGAGTAAA GATCGTCGTC GTCAAGACTC GGATGATGAG GATCGGAAAG 6661 GTCGTGAACG TCGGGAAGAT TCAGGGGAAA GACGTCGCGG AGATCGGGAT CGACGTGATC 6721 GAAACAAGGA TCAGGAGGAT CACCGTGAAG ATCGCCGTGA CCGAAGCAAG GATCGTGAGG 6781 ATCGACGTGA TCGCCGTCGT CATGACTCTG ATGATGATCG TAAAACTCGT CGGGATAGAA 6841 GTGAAGAGCG AGGAGGACGT CGTCGTGAAG TGGAATCGGA TGATCGACGC CGACGTCGTT 6901 GAATTTTCAA ATTTTAAATA CTGAATATTT GTTTTTTTTC CTATTATTTA TTTATTCTCT 6961 TTGTGTTTTT TTTCTTGCTT TCTAAAAAAT TAATTCAATC CAAATCTAAa catttttttt 7021 tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat gtgcaagttt 7081 atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa gggggaaaca 7141 cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat tcaattcaga 7201 aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag gaaaactagt 7261 aaaaaataag caaagaaatt aggcgaacga tgagaattgt cctcgcttgg caaatgcgaa 7321 tccgtatgga gaggcacgtt tggcgaaggc aaatgttcgg tatggagatc tgtaaaaatt 7381 tttaagttga aatttggtgt tgctctttta caaaattttc cgattttcgc ttgaaattac 7441 ggtgccaggt ctcgacacgt cttccaattt ttcaaattca aaagagcctt taatgggctg 7501 tagttgctaa tttctcgttt ttgaaaattt ttcttccgtt taatcgaaat ttgatgtatt 7561 ttatttatga ttttcaataa atttcaaaga aactggtgaa aactcggaaa attgtgaact 7621 acagtaatcc aatccttaaa ggcgcacacc ttttaaatgt ccgccccaat acgatatttt 7681 tttaagattc gggcccTCTA GAGCGGCCGC CACCGCGGTG GAGCTCCAAT TCGCCCTATA 7741 GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 7801 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCCTT CGCCAGCTGG CGTAATAGCG 7861 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGTAG CCTGAATGGC GAATGGGACG 7921 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 7981 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 8041 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 8101 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 8161 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 8221 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 8281 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 8341 CGAATTTTAA CAAAATATTA ACGTTTACAA TTTCAGGTGG CACTTTTCGG GGAAATGTGC 8401 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 8461 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 8521 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 8581 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 8641 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 8701 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 8761 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 8821 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 8881 GCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 8941 TAACCGCTTT TTTTCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 9001 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 9061 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 9121 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 9181 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 9241 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGCAGTCAGG 9301 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 9361 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 9421 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 9481 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 9541 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 9601 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 9661 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 9721 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 9781 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 9841 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 9901 CCGAACTGAG ATACCTACAG CGTGAGCATT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 9961 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 10021 CAGGGGGGAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 10081 GTCGATTTTT GTGATGCTCG TCAGGGGGGC CGAGCCTATG GAAAAACGCC AGCAACGCGG 10141 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 10201 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA 10261 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA 10321 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG 10381 ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTACCTCACT CATTAGGCAC 10441 CCCAGGCTTT ACACTTTATG CTTCCGGCTC CTATGTTGTG TGGAATTGTG AGCGGATAAC 10501 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTCGGAAT // 1511 LOCUS ADDGENE_1511 4913 bp DNA circular SYN 01/03/2005 DEFINITION L2603 complete plasmid sequence KEYWORDS . SOURCE L2603 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1511 at www.addgene.org FEATURES Location/Qualifiers source 1..4913 /organism="Addgene plasmid L2603" /mol_type="other DNA" misc_feature 69..110 /label="SynIVS.A" misc_feature 136..201 /label="ATG-SecSig" misc_feature 216..240 /label="pes10.delta-181" misc_feature 377..399 /label="pes10.Tsa" misc_feature 439..480 /label="SynIVS.A" misc_feature 482..499 /label="rf22.11" misc_feature 518..544 /label="SV40.NLS" CDS 518..1429 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 560..1420 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 731..781 /label="SynIVS.R" misc_feature 803..822 /label="gfp.S65C" misc_feature 936..986 /label="SynIVS.S" misc_feature 1151..1201 /label="SynIVS.T" misc_feature 1515..1565 /label="SynIVS.L" promoter 2693..2721 /label="Amp_promoter" CDS 2763..3623 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2763..3623 /label="Ampicillin" /gene="Ampicillin" rep_origin 3778..4397 /label="pBR322_origin" misc_feature 4613..4639 /label="lacI.C" promoter 4706..4735 /label="lac_promoter" misc_feature 4770..4788 /label="M13r_primer" misc_feature 4802..4852 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGAGGAT CCccgGGGAT TGGCCAAAGG 61 ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT 121 GCTTGGctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca 181 gcatccgcac tggcagtctc cgaaccggCC TGCAGGATCG ATTTTTTGCA AATTACGAGC 241 GTTGTAGGGG GCGGAgCGAT AGGTCCTATA GGTTTTgGTA TATCATCATT CATTCATTCA 301 TTGGTACATT CATTTACCCA CCTTCCTCTT TCTGAGCTTC TCTGGAGTTC TGTGCTTCCT 361 TTTTCCCTTA TCTTTATACT GTAATTTTTA ACTTTCAGgc attgattgGA TCCCCGGGAT 421 TGGCCAAAGG ACCCAAAGgt atgtttcgaa tgatactaac ataacataga acattttcag 481 GAGGACCCTT GCTTGGAGGG TACCGAGCTC AGAAAAAATG ACTGCTCCAA AGAAGAAGCG 541 TAAGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT 601 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA 661 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC 721 TGTTCCATGG gtaagtttaa acatatatat actaactaac cctgattatt taaattttca 781 gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC TTcTCgAGAT ACCCAGATCA 841 TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC 901 TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag tttaaacagt tcggtactaa 961 ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG TTTGAAGGTG ATACCCTTGT 1021 TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT GGAAACATTC TTGGACACAA 1081 ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG 1141 AATCAAAGTT gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca 1201 gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG ACCATTATCA 1261 ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT ACCTGTCCAC 1321 ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGTCC TTCTTGAGTT 1381 TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA TACAAATAGC ATTCGTAGAA 1441 TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG 1501 CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt 1561 ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC 1621 ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA 1681 ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA 1741 AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA 1801 ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT 1861 GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA AAATACCTTA TCATATGTTA 1921 CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT CTGGGCCTCT CATGACGTCA 1981 AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA ATTTTTCAAT CAAGTGAAAG 2041 TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT CCCCTATTGT TTGTCAAGAT 2101 TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG ATGAGCACGA TGCAAGAAAG 2161 ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA GTAGAAGTTG ATAATTTGAA 2221 AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG AATACATTCC CAATATACCA 2281 AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA 2341 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC 2401 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG 2461 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA 2521 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGGCCTTAAG GGCCTCGTGA 2581 TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA 2641 CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA 2701 TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA 2761 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 2821 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 2881 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 2941 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 3001 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 3061 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 3121 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 3181 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 3241 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 3301 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 3361 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 3421 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 3481 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 3541 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 3601 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 3661 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA 3721 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA 3781 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 3841 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA 3901 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT 3961 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT 4021 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT 4081 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 4141 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCATTGA GAAAGCGCCA 4201 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG 4261 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC 4321 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA 4381 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 4441 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG 4501 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG 4561 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT 4621 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT 4681 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG 4741 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 4801 Tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agAGCTTAAA 4861 AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC TTGGAAATGA AAT // 1512 LOCUS ADDGENE_1512 4693 bp DNA circular SYN 01/03/2005 DEFINITION L2604 complete plasmid sequence KEYWORDS . SOURCE L2604 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1512 at www.addgene.org FEATURES Location/Qualifiers source 1..4693 /organism="Addgene plasmid L2604" /mol_type="other DNA" misc_feature 32..56 /label="myo2.delta-90" misc_feature 123..146 /label="myo2.5'" misc_feature 220..261 /label="SynIVS.A" misc_feature 298..324 /label="SV40.NLS" CDS 298..1209 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 340..1200 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 511..561 /label="SynIVS.R" misc_feature 583..602 /label="gfp.S65C" misc_feature 716..766 /label="SynIVS.S" misc_feature 931..981 /label="SynIVS.T" misc_feature 1295..1345 /label="SynIVS.L" promoter 2473..2501 /label="Amp_promoter" CDS 2543..3403 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2543..3403 /label="Ampicillin" /gene="Ampicillin" rep_origin 3558..4177 /label="pBR322_origin" misc_feature 4393..4419 /label="lacI.C" promoter 4486..4515 /label="lac_promoter" misc_feature 4550..4568 /label="M13r_primer" misc_feature 4582..4632 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAC TCTAGACACT TTTCAGCTAC CTAGATACAT 61 GGATATCCCC GCCTCCCAAT CCACCCACCC AGGGAAAAAG AAGGGCTCGC CGAAAATCAA 121 AGTTATCTCC AGGCTCGCGC ATCCCACCGA GCGGTTGACT TCTCTCCACC ACTTTTCATT 181 TTAACCCTCG GGGTACGGGA TTGGCCAAAG GACCCAAAGG TATGTTTCGA ATGATACTAA 241 CATAACATAG AACATTTTCA GGAGGACCct tCTTGGAGGG TACCGAGCTC AGAAAAAATG 301 ACTGCTCCAA AGAAGAAGCG TAAGGTACCG GTAGAAAAAA TGAGTAAAGG AGAAGAACTT 361 TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT 421 TCTGTCAGTG GAGAGGGTGA AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT 481 TGCACTACTG GAAAACTACC TGTTCCATGG gtaagtttaa acatatatat actaactaac 541 cctgattatt taaattttca gCCAACACTT GTCACTACTT TCTgTTATGG TGTTCAATGC 601 TTcTCgAGAT ACCCAGATCA TATGAAACgG CATGACTTTT TCAAGAGTGC CATGCCCGAA 661 GGTTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACgtaag 721 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagGTGC TGAAGTCAAG 781 TTTGAAGGTG ATACCCTTGT TAATAGAATC GAGTTAAAAG GTATTGATTT TAAAGAAGAT 841 GGAAACATTC TTGGACACAA ATTGGAATAC AACTATAACT CACACAATGT ATACATCATG 901 GCAGACAAAC AAAAGAATGG AATCAAAGTT gtaagtttaa acatgatttt actaactaac 961 taatctgatt taaattttca gAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT 1021 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA 1081 GACAACCATT ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC 1141 CACATGGTCC TTCTTGAGTT TGTAACAGCT GCTGGGATTA CACATGGCAT GGATGAACTA 1201 TACAAATAGC ATTCGTAGAA TTCCAACTGA GCGCCGGTCG CTACCATTAC CAACTTGTCT 1261 GGTGTCAAAA ATAATAGGGG CCGCTGTCAT CAGAgtaagt ttaaactgag ttctactaac 1321 taacgagtaa tatttaaatt ttcagCATCT CGCGCCCGTG CCTCTGACTT CTAAGTCCAA 1381 TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT CCCACCCCCT ATTTTTGTTA 1441 TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT CTTTTAAGTC ACCTCTAACA 1501 ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT AAAAAGTCGA AAAAAATTGT 1561 GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT ACACAATGTT CTGTGTACAC 1621 TTCTTATGTT TTTTACTTCT GATAAATTTT TTTGAAACAT CATAGAAAAA ACCGCACACA 1681 AAATACCTTA TCATATGTTA CGTTTCAGTT TATGACCGCA ATTTTTATTT CTTCGCACGT 1741 CTGGGCCTCT CATGACGTCA AATCATGCTC ATCGTGAAAA AGTTTTGGAG TATTTTTGGA 1801 ATTTTTCAAT CAAGTGAAAG TTTATGAAAT TAATTTTCCT GCTTTTGCTT TTTGGGGTTT 1861 CCCCTATTGT TTGTCAAGAT TTCGAGGACG GCGTTTTTCT TGCTAAAATC ACAAGTATTG 1921 ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG TGGAAGGTGA 1981 GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT TAAATGACAG 2041 AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA CGGGCCCTTT 2101 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 2161 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 2221 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 2281 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 2341 CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT 2401 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 2461 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 2521 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 2581 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA 2641 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 2701 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT 2761 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT 2821 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 2881 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC 2941 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 3001 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 3061 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC 3121 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 3181 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC 3241 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 3301 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 3361 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA 3421 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA 3481 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 3541 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 3601 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 3661 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 3721 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 3781 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 3841 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 3901 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 3961 TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 4021 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 4081 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 4141 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 4201 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 4261 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 4321 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 4381 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 4441 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 4501 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA 4561 TGACCATGAT TACGCCAAGC Tgtaagttta aacatgatct tactaactaa ctattctcat 4621 ttaaattttc agAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA CGCTAACAAC 4681 TTGGAAATGA AAT // 1513 LOCUS ADDGENE_1513 6141 bp DNA circular SYN 01/03/2005 DEFINITION L2605 complete plasmid sequence KEYWORDS . SOURCE L2605 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1513 at www.addgene.org FEATURES Location/Qualifiers source 1..6141 /organism="Addgene plasmid L2605" /mol_type="other DNA" misc_feature 25..49 /label="glp1.delta-1635" misc_feature 1634..1661 /label="glp1.Tsa" misc_feature 1746..1772 /label="SV40.NLS" CDS 1746..2657 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 1788..2648 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1959..2009 /label="SynIVS.R" misc_feature 2031..2050 /label="gfp.S65C" misc_feature 2164..2214 /label="SynIVS.S" misc_feature 2379..2429 /label="SynIVS.T" misc_feature 2743..2793 /label="SynIVS.L" promoter 3921..3949 /label="Amp_promoter" CDS 3991..4851 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3991..4851 /label="Ampicillin" /gene="Ampicillin" rep_origin 5006..5625 /label="pBR322_origin" misc_feature 5841..5867 /label="lacI.C" promoter 5934..5963 /label="lac_promoter" misc_feature 5998..6016 /label="M13r_primer" misc_feature 6030..6080 /label="decoy" ORIGIN 1 AAGCTTGCAT GCCTGCAGGC CTTGGTCGAG TATGAGAAAC AACATTAACA ATGTTTTGGG 61 AGAAACATGT AGTTTTGTAC GTCCGGTCCA GTGAGAGTTT TCAACTTATT TTGGAAGAAA 121 TTTTTTTTTG ACAAAATTTT GCAATTTCCC ACGCAGAAAT ACGGTATGCC CCGTATCTCG 181 CCACGAACAT AATATTTTAA ATTGACGGAA AGGTGTGCGC CTTTAAGAAG CACAGTAACC 241 ATTTTCCGGT TTTTTTTTTG AAATTGCCTT TGATTTTTAC TTTTTTCTCC CAAAAACGAG 301 AAATTTAATA TAATCCATCA AAATCTTTGT GAACGAGAGA AATATAGTGA AAAACTCAAA 361 GAAAATTGCG AAATGGATTA CTGTACTCCA TAAAGGCGCA CATCTTTTCG AAAAATCGTG 421 AAAAATTGCA TCTGACCAAC AGTTTTGCGT GGAATCTTCC TAGATTCAAA TTTCCTATGA 481 GTCATAAAAC TATCATTTTA TCGGACAATA ACTACACGTG TACCTTGTTT CCATATGTTC 541 TACCAATATA ATTTGTGTTC ATATGTGGCC AGGTACGGGA AATTAGAACA CGGACTGATT 601 TAGGAAGGTT CAAATTCGAG AACAATGGAC GACTTGCTGC ACGAAAACGT GGATTATTCT 661 GAAACCGCCT ATGTGATCAC TATCGATTTA GCACTTCGGT TTTCTTTTTC CGTTTTCCTT 721 TATATTCCGT ATTGTATGCC GACAATAACG CTTTTTCTTT GTATAAACAT TTCCAGAAGC 781 AGAGCAAATT TGTTTATTCC AGAATTGACT GAATATTGAA ATAATGTTTA CAGACATACT 841 GTAGATTTTT TTCTATTCAA ACATGAATAC AGTTTTTCGT ATTTTTAATC TTCAGATAAG 901 TTTTGTGTGT GTTTTTGAGA ATAATATTTA AAAAAGATAA GCAAATATCA GAACTACGGT 961 AAGCGAAGGG GTACGGTCGT CAATTGCGGA CTTCCGAGCT GTGCACGGCG CCAAAATTTA 1021 TTTTGAGTTT TAAAATGATT TTCTTTGTTT TTTTTTCTTT TTTTGACACG ATAAGAACAT 1081 TTTTCTGTAT TTCTAATATT TTAAATGAGA TCGTAATAAG TTTATTTAGC TCCATCACAT 1141 TATTTTCGGA ATATTCATCG CTTGAAAACG GCGTAAAATA GGTTCCAAGT AAATCATTGC 1201 AAACAAATCT CCGTTTATCG TTCTAACAAA GTTTTTCGTT CACTTTCTTA TGTAAATCGT 1261 TTTCCCCACT TCTTCTTCAC GGGAATGATC AATTGAAAGG CCATAAATTG GTTGTTTTCT 1321 CCACATTTCG CAACCAACAT GCAGCTGTCG GATAGGCAAT AAAATAATGT AATCAATGAG 1381 TTTTCCGATT TATCCTAACA GTTCTTTTTC TAGTGTTCCT ATTCGAAAAA AGTACTGTTA 1441 TCCTTCTTTT TGGACCGCCC ATCTCAGGTG TCAAGACAAT CGCAACACAC TCAGAAAATG 1501 GGCGGAGTAT GCCACCCGGC CTTCATTCGT CGCTCCTTGT CTTCTCCATT TTCGTTTTTT 1561 TCCTGTTCTC CGTTCTTTTT TTGTACTTTT GTGATAATGC TGAAGTGTTC TTTTTTTTCG 1621 CTTAACCGCA TCTCTTGATT TAATTCATAT TTTCTTTCAG ATTCATaTAC AATAACACCA 1681 AGAGCTGCTC TAACAACTAA TAATAACAGA ATCTAGAGGA TCCCCGGGTA CCGAGCTCAG 1741 AAAAAATGAC TGCTCCAAAG AAGAAGCGTA AGGTACCGGT AGAAAAAATG AGTAAAGGAG 1801 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC 1861 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA 1921 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGgt aagtttaaac atatatatac 1981 taactaaccc tgattattta aattttcagC CAACACTTGT CACTACTTTC TgTTATGGTG 2041 TTCAATGCTT cTCgAGATAC CCAGATCATA TGAAACgGCA TGACTTTTTC AAGAGTGCCA 2101 TGCCCGAAGG TTATGTACAG GAAAGAACTA TATTTTTCAA AGATGACGGG AACTACAAGA 2161 CACgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagGTGCTG 2221 AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA 2281 AAGAAGATGG AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT 2341 ACATCATGGC AGACAAACAA AAGAATGGAA TCAAAGTTgt aagtttaaac atgattttac 2401 taactaacta atctgattta aattttcagA ACTTCAAAAT TAGACACAAC ATTGAAGATG 2461 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC 2521 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA 2581 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 2641 ATGAACTATA CAAATAGCAT TCGTAGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 2701 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 2761 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 2821 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 2881 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 2941 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 3001 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 3061 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 3121 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 3181 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 3241 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 3301 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 3361 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 3421 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 3481 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 3541 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 3601 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 3661 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 3721 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 3781 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 3841 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 3901 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3961 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 4021 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 4081 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 4141 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 4201 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 4261 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 4321 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 4381 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 4441 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 4501 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 4561 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 4621 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 4681 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 4741 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 4801 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 4861 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 4921 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 4981 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 5041 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 5101 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 5161 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 5221 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 5281 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 5341 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 5401 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 5461 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 5521 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 5581 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 5641 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 5701 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 5761 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 5821 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 5881 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 5941 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 6001 AACAGCTATG ACCATGATTA CGCCAAGCTg taagtttaaa catgatctta ctaactaact 6061 attctcattt aaattttcag AGCTTAAAAA TGGCTGAAAT CACTCACAAC GATGGATACG 6121 CTAACAACTT GGAAATGAAA T // 1514 LOCUS ADDGENE_1514 5740 bp DNA circular SYN 01/03/2005 DEFINITION L2630 complete plasmid sequence KEYWORDS . SOURCE L2630 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1514 at www.addgene.org FEATURES Location/Qualifiers source 1..5740 /organism="Addgene plasmid L2630" /mol_type="other DNA" misc_feature complement(96..126) /label="unc54.enh" misc_feature 164..188 /label="myo2.-1090" CDS 1139..2335 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf1" /translation="MVREQCADTFQLPRITIGYPRLPIHPPREKEGLAENQSYLQARA SHRAVDFSPPLFILTLGVRDWPKDPKVCFE*Y*HNIEHFQEDPWRVPSSEKMTAPKKK RKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTG KLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGY VQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKED GNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDG SVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHG MDELYK" misc_feature 1252..1275 /label="myo2.5'" misc_feature 1349..1390 /label="SynIVS.A" misc_feature 1424..1450 /label="SV40.NLS" gene 1466..2326 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 1637..1687 /label="SynIVS.R" misc_feature 1709..1728 /label="gfp.S65C" misc_feature 1842..1892 /label="SynIVS.S" misc_feature 2057..2107 /label="SynIVS.T" misc_feature 2349..2399 /label="SynIVS.M" misc_feature 2476..2526 /label="SynIVS.L" promoter 3654..3682 /label="Amp_promoter" gene 3724..4584 /label="Ampicillin" /gene="Ampicillin" CDS 3724..4584 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4739..5358 /label="pBR322_origin" misc_feature 5574..5600 /label="lacI.C" promoter 5667..5696 /label="lac_promoter" misc_feature 5731..5749 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCGTCATCCA 61 CAGTGTAATG TAAGATGGTT CAGAGATTCA GAAGAGAGGC AGAGATAGCC TCATCCCACT 121 CAATTGATGA GAGAATGGGA TCCCCAGCTT GCATGCCTGC AGGTCGAGGC ATTTGAATTG 181 GGGGTGGTGG ACAGTAACTG TCTGTAATAA TAATTACTCC TGACCAGGTT GCAATTCGAG 241 TTTTGATAAG CATAATTATA CCTTGTACAT TGTGGGTTTT GTGCTGTGGA CGTTTTATTG 301 TGACATCCCC ATAAGCTACA AGAACAAAAA TGAAATgTAA AAGTATTGAA AACGTCGTAC 361 ATTTTATATC TGAGTAGTAT CCTTTGCTTT AAATGTCCAT AAAAcTAATT TTATAATCAA 421 TAAAACAACG TTTGTAAATC AACTGAGTTT ACAAGTAGAG ACATTGAGGG ATACTTTCAC 481 TATGCTAAAG TGATAATCGA CCAAATAATA ACTtCACTTT GGTATTTATT CCTGTCTTAT 541 AAaTGTTATG TATGAATTAA ATTCATATGC ATATGGCTCA CTCTGACAAt ttttAATAAT 601 CTTCCAGATC AATATTGACT ACCGATGCGG GTGGTCTTTT GCTTTGAATT CTGCTGAACT 661 TTACACCCCG AACAGCAATG TGTGCTTCAG CcTAAAAAAA AGTAAGTGTG TTAATCAGTg 721 CCCCCGATTC TTCATTTTTT GCCCCTCTCT CCCGTTTCGT CGGCAAAAGA AGAGAAAATA 781 AAGATAAGTC TCAAGATAGG TTGGTAATCG CTAAAGTGGT TGTGTGGATA AGAGTAGCAA 841 AATGGCAGGA AGAGCACTTT GCGCGCACAC ACTGTACTCA TTGTTCTGGA TAAAATTCTC 901 TCGTTGTTTG CCGTCGGATG TCTGCCTCTC TGCATTGAGC CGGCTTCTTC ACTATCTTTA 961 GTTAACCTAA AATGCCGTTT CTTTTCTCGT ATCCCcACTA TcCCGTTGAG GTTCTCTGCT 1021 CTCTTCGCTC CCTACCGCCA GCGAGCAACT ATCCGTGGGG GCGCCTTGCT CGGAAGATGG 1081 GGGGGAAGAA AGAAGATTTT TGCTATTTGC ACTTGAGAAA GAGACTTTTC CTGCGTCGAT 1141 GGTTAGAGAA CAGTGTGCAG ACACTTTTCA GCTACCTAGA atTACAatTG GATATCCCCG 1201 CCTCCCAATC CACCCACCCA GGGAAAAAGA AGGGCTCGCC GAAAATCAAA GTTATCTCCA 1261 GGCTCGCGCA TCCCACCGAG CGGTTGACTT CTCTCCACCA CTTTTCATTT TAACCCTCGG 1321 GGTACGGGAT TGGCCAAAGG ACCCAAAGGT ATGTTTCGAA TGATACTAAC ATAACATAGA 1381 ACATTTTCAG GAGGACCCTT GGAGGGTACC GAGCTCAGAA AAAATGACTG CTCCAAAGAA 1441 GAAGCGTAAG GTACCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT 1501 CCCAATTCTT GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA 1561 GGGTGAAGGT GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA 1621 ACTACCTGTT CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa 1681 ttttcagCCA ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC 1741 AGATCATATG AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA 1801 AAGAACTATA TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg 1861 tactaactaa ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC 1921 CCTTGTTAAT AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG 1981 ACACAAATTG GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA 2041 GAATGGAATC AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa 2101 ttttcagAAC TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA 2161 TTATCAACAA AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT 2221 GTCCACACAA TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT 2281 TGAGTTTGTA ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC 2341 GTAGAATTgt aagtttaaac tttctcatac taactaacgc gctctattta aattttcagA 2401 ATTCCAACTG AGCGCCGGTC GCTACCATTA CCAACTTGTC TGGTGTCAAA AATAATAGGG 2461 GCCGCTGTCA TCAGAgtaag tttaaactga gttctactaa ctaacgagta atatttaaat 2521 tttcagCATC TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA 2581 CATGCTCTTT CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT 2641 AATTTCTTTG TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA 2701 AAAATAGAAT TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT 2761 AATAATTCTA TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC 2821 TGATAAATTT TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT 2881 ACGTTTCAGT TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC 2941 AAATCATGCT CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA 3001 GTTTATGAAA TTAATTTTCC TGCTTTTGCT TTTTGGGGTT TCCCCTATTG TTTGTCAAGA 3061 TTTCGAGGAC GGCGTTTTTC TTGCTAAAAT CACAAGTATT GATGAGCACG ATGCAAGAAA 3121 GATCGGAAGA AGGTTTGGGT TTGAGGCTCA GTGGAAGGTG AGTAGAAGTT GATAATTTGA 3181 AAGTGGAGTA GTGTCTATGG GGTTTTTGCC TTAAATGACA GAATACATTC CCAATATACC 3241 AAACATAACT GTTTCCTACT AGTCGGCCGT ACGGGCCCTT TCGTCTCGCG CGTTTCGGTG 3301 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 3361 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 3421 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG 3481 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGGCCTTAA GGGCCTCGTG 3541 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC 3601 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 3661 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG 3721 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT 3781 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT 3841 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 3901 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 3961 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC 4021 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA 4081 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG 4141 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 4201 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 4261 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA 4321 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG 4381 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG 4441 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 4501 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 4561 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT 4621 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC 4681 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG 4741 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA 4801 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 4861 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG 4921 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG 4981 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA 5041 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC 5101 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC 5161 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA 5221 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT 5281 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG 5341 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC 5401 ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 5461 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG 5521 GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC 5581 TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT 5641 TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT 5701 GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT // 1515 LOCUS ADDGENE_1515 7525 bp DNA circular SYN 01/03/2005 DEFINITION L2678 complete plasmid sequence KEYWORDS . SOURCE L2678 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1515 at www.addgene.org FEATURES Location/Qualifiers source 1..7525 /organism="Addgene plasmid L2678" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..1280 /label="Orf2" /translation="MTAPKKKRKVPVGEDQKQHLELSRDIAQRFNALYGEIDPVVLQR RDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDIIFSL NGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSLIKLI LTNKI*LIFRTVVCRLNLT" gene 684..4237 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 818..868 /label="SynIVS.B" CDS 1319..4240 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1496..1546 /label="SynIVS.D" misc_feature 1937..1987 /label="SynIVS.E" misc_feature 2244..2294 /label="SynIVS.F" misc_feature 2525..2575 /label="SynIVS.G" misc_feature 2812..2862 /label="SynIVS.H" misc_feature 3178..3228 /label="SynIVS.I" misc_feature 3489..3539 /label="SynIVS.J" misc_feature 3895..3945 /label="SynIVS.K" misc_feature 4261..4311 /label="SynIVS.L" promoter 5439..5467 /label="Amp_promoter" CDS 5509..6369 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5509..6369 /label="Ampicillin" /gene="Ampicillin" rep_origin 6524..7143 /label="pBR322_origin" misc_feature 7359..7385 /label="lacI.C" promoter 7452..7481 /label="lac_promoter" misc_feature 7516..7534 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaCCGG 601 TGGGTGAAGA CCAGAAACAG CACCTCGAAC TGAGCCGCGA TATTGCCCAG CGTTTCAACG 661 CGCTGTATGG CGAGATCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 721 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 781 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGaAGgta agtttaaaca gatccatact 841 aactaacttg ttctgacata attttcagCt TGAATGGCGA ATGGCGCTTT GCCTGGTTTC 901 CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG 961 TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA 1021 CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT 1081 CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG 1141 ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT TACGGCCAGg 1201 taagtttaat taagttgata CTAACTAACA AGATCTGATT AATTTTCagG ACAGTCGTTT 1261 GCCGTCTGAA TTTGACCTGA GCGCATTTTT ACGCGCCGGA GAAAACCGCC TCGCGGTGAT 1321 GGTGCTGCGC TGGAGTGACG GCAGTTATCT GGAAGATCAG GATATGTGGC GGATGAGCGG 1381 CATTTTCCGT GACGTCTCGT TGCTGCATAA ACCGACTACA CAAATCAGCG ATTTCCATGT 1441 TGCCACTCGC TTTAATGATG ATTTCtccCG CGCTGTACTG GAGGCTGAAG TcCAGgtaag 1501 tttaaacagg atcttactaa ctaacatgct aacactgaat tttcagATGT GCGGCGAGTT 1561 GCGTGACTAC CTACGGGTAA CAGTTTCTTT ATGGCAGGGT GAAACGCAGG TCGCCAGCGG 1621 CACCGCGCCT TTCGGCGGTG AAATTATCGA TGAGCGTGGT GGTTATGCCG ATCGCGTCAC 1681 ACTACGTCTG AACGTCGAAA ACCCGAAACT GTGGAGCGCC GAAATCCCGA ATCTCTATCG 1741 TGCGGTGGTT GAACTGCACA CCGCCGACGG CACGCTGATT GAAGCAGAAG CCTGCGATGT 1801 CGGTTTCCGC GAGGTGCGGA TTGAAAATGG TCTGCTGCTG CTGAACGGCA AGCCGTTGCT 1861 GATTCGAGGC GTTAACCGTC ACGAGCATCA TCCTCTGCAT GGTCAGGTCA TGGATGAGCA 1921 GACGATGGTG CAGGATgtaa gtttaaacta ttcgttacta actaacttta aacatttaaa 1981 ttttcagATC CTGCTGATGA AGCAGAACAA CTTTAACGCC GTGCGCTGTT CGCATTATCC 2041 GAACCATCCG CTGTGGTACA CGCTGTGCGA CCGCTACGGC CTGTATGTGG TGGATGAAGC 2101 CAATATTGAA ACCCACGGCA TGGTGCCAAT GAATCGTCTG ACCGATGATC CGCGCTGGCT 2161 ACCGGCGATG AGCGAACGCG TAACGCGAAT GGTGCAGCGC GATCGTAATC ACCCGAGTGT 2221 GATCATCTGG TCGCTGGGGA ATGgtaagtt taaacagttg aatactaact aacggagatc 2281 tttgaaattt tcagAATCAG GCCACGGCGC TAATCACGAC GCGCTGTATC GCTGGATCAA 2341 ATCTGTCGAT CCTTCCCGCC CGGTGCAGTA TGAAGGCGGC GGAGCCGACA CCACGGCCAC 2401 CGATATTATT TGCCCGATGT ACGCGCGCGT GGATGAAGAC CAGCCCTTCC CGGCTGTGCC 2461 GAAATGGTCC ATCAAAAAAT GGCTTTCGCT ACCTGGAGAG ACGCGCCCGC TGATtCTTTG 2521 CGAGgtaagt ttaaacagaa ctctactaac taacacatta gatcctaatt ttcagTACGC 2581 tCACGCGATG GGcAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT TTCGTCAGTA 2641 TCCCCGTTTA CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA TTAAATATGA 2701 TGAAAACGGC AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC CGAACGATCG 2761 CCAGTTCTGT ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG Cgtaagttta 2821 aacaataacc tactaactaa cgtagataat ttaaattttc agGCTGACGG AAGCAAAACA 2881 CCAGCAGCAG TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA 2941 CCTGTTCCGT CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC 3001 GCTGGCAAGC GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT 3061 GCCTGAACTA CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA 3121 ACCGAACGCG ACCGCATGGT CAGAAGCCGG GCACATCAGC GCaTGGCAGC AGTGGAGgta 3181 agtttaaaca agatcctact aactaactct acattgatga attttcagAC TGGCGGAAAA 3241 CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT 3301 GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT 3361 TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC 3421 CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC 3481 CTGGGTCGgt aagtttaaac aaagttgtac taactaacga agatcttgat aattttcagA 3541 ACGCTGGAAG GCGGCGGGCC ATTACCAGGC CGAAGCAGCG TTGTTGCAGT GCACGGCAGA 3601 TACACTTGCT GATGCGGTGC TGATTACGAC CGCTCACGCG TGGCAGCATC AGGGGAAAAC 3661 CTTATTTATC AGCCGGAAAA CCTACCGGAT TGATGGTAGT GGTCAAATGG CGATTACCGT 3721 TGATGTTGAA GTGGCGAGCG ATACACCGCA TCCGGCGCGG ATTGGCCTGA ACTGCCAGCT 3781 GGCGCAGGTA GCAGAGCGGG TAAACTGGCT CGGATTAGGG CCGCAAGAAA ACTATCCCGA 3841 CCGCCTTACT GCCGCCTGTT TTGACCGCTG GGATCTGCCA TTGTCAGACA TGTAgtaagt 3901 ttaaacttga tagtactaac taacatgttt catttaaatt ttcagTACCC CGTACGTCTT 3961 CCCGAGCGAA AACGGTCTGC GCTGCGGGAC GCGCGAATTG AATTATGGCC CACACCAGTG 4021 GCGCGGCGAC TTCCAGTTCA ACATCAGCCG CTACAGTCAA CAGCAACTGA TGGAAACCAG 4081 CCATCGCCAT CTGCTGCACG CGGAAGAAGG CACATGGCTG AATATCGACG GTTTCCATAT 4141 GGGGATTGGT GGCGACGACT CCTGGAGCCC GTCAGTATCG GCGGAATTCC AACTGAGCGC 4201 CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC TGTCATCAGA 4261 gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca gCATCTCGCG 4321 CCCGTGCCTC TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC TCTTTCTCCC 4381 TGTGCTCCCA CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT CTTTGTTTTT 4441 TAGCTTCTTT TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT AGAATTAATT 4501 CGTAATAAAA AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA TTCTATCCCA 4561 AAATCTACAC AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA AATTTTTTTG 4621 AAACATCATA GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT TCAGTTTATG 4681 ACCGCAATTT TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC ATGCTCATCG 4741 TGAAAAAGTT TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA TGAAATTAAT 4801 TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG AGGACGGCGT 4861 TTTTCTTGCT AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG GAAGAAGGTT 4921 TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG GAGTAGTGTC 4981 TATGGGGTTT TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA TAACTGTTTC 5041 CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC 5101 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA 5161 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 5221 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT 5281 GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG CCTATTTTTA 5341 TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT 5401 GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG 5461 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA 5521 CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC 5581 CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC 5641 ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT 5701 CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC 5761 GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA 5821 CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC 5881 ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG 5941 GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA 6001 CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG 6061 GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA 6121 TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG 6181 GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT 6241 GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT 6301 CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG 6361 CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT 6421 TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT 6481 TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT 6541 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA 6601 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC 6661 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC 6721 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT 6781 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG 6841 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC 6901 TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG 6961 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG 7021 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT 7081 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC 7141 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG 7201 TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC 7261 CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA 7321 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT 7381 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG 7441 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA 7501 TAACAATTTC ACACAGGAAA CAGCT // 1516 LOCUS ADDGENE_1516 4828 bp DNA circular SYN 01/03/2005 DEFINITION L2680 complete plasmid sequence KEYWORDS . SOURCE L2680 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1516 at www.addgene.org FEATURES Location/Qualifiers source 1..4828 /organism="Addgene plasmid L2680" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..1478 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYK" gene 609..1469 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 780..830 /label="SynIVS.R" misc_feature 852..871 /label="gfp.S65C" misc_feature 985..1035 /label="SynIVS.S" misc_feature 1200..1250 /label="SynIVS.T" misc_feature 1564..1614 /label="SynIVS.L" promoter 2742..2770 /label="Amp_promoter" gene 2812..3672 /label="Ampicillin" /gene="Ampicillin" CDS 2812..3672 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3827..4446 /label="pBR322_origin" misc_feature 4662..4688 /label="lacI.C" promoter 4755..4784 /label="lac_promoter" misc_feature 4819..4837 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaCCGG 601 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 661 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 721 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 781 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 841 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 901 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 961 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 1021 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 1081 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 1141 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 1201 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 1261 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 1321 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 1381 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 1441 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATAGCA TTCGTAGAAT TCCAACTGAG 1501 CGCCGGTCGC TACCATTACC AACTTGTCTG GTGTCAAAAA TAATAGGGGC CGCTGTCATC 1561 AGAgtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagCATCTC 1621 GCGCCCGTGC CTCTGACTTC TAAGTCCAAT TACTCTTCAA CATCCCTACA TGCTCTTTCT 1681 CCCTGTGCTC CCACCCCCTA TTTTTGTTAT TATCAAAAAA CTTCTCTTAA TTTCTTTGTT 1741 TTTTAGCTTC TTTTAAGTCA CCTCTAACAA TGAAATTGTG TAGATTCAAA AATAGAATTA 1801 ATTCGTAATA AAAAGTCGAA AAAAATTGTG CTCCCTCCCC CCATTAATAA TAATTCTATC 1861 CCAAAATCTA CACAATGTTC TGTGTACACT TCTTATGTTT TTTACTTCTG ATAAATTTTT 1921 TTGAAACATC ATAGAAAAAA CCGCACACAA AATACCTTAT CATATGTTAC GTTTCAGTTT 1981 ATGACCGCAA TTTTTATTTC TTCGCACGTC TGGGCCTCTC ATGACGTCAA ATCATGCTCA 2041 TCGTGAAAAA GTTTTGGAGT ATTTTTGGAA TTTTTCAATC AAGTGAAAGT TTATGAAATT 2101 AATTTTCCTG CTTTTGCTTT TTGGGGTTTC CCCTATTGTT TGTCAAGATT TCGAGGACGG 2161 CGTTTTTCTT GCTAAAATCA CAAGTATTGA TGAGCACGAT GCAAGAAAGA TCGGAAGAAG 2221 GTTTGGGTTT GAGGCTCAGT GGAAGGTGAG TAGAAGTTGA TAATTTGAAA GTGGAGTAGT 2281 GTCTATGGGG TTTTTGCCTT AAATGACAGA ATACATTCCC AATATACCAA ACATAACTGT 2341 TTCCTACTAG TCGGCCGTAC GGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA 2401 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 2461 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT 2521 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA 2581 GATGCGTAAG GAGAAAATAC CGCATCAGGC GGCCTTAAGG GCCTCGTGAT ACGCCTATTT 2641 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA 2701 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC 2761 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT 2821 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT 2881 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT 2941 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT 3001 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC 3061 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC 3121 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT 3181 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG 3241 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG 3301 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA 3361 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA 3421 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT 3481 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC 3541 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG 3601 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT 3661 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT 3721 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC 3781 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT 3841 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA 3901 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC 3961 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC 4021 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT 4081 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT 4141 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG 4201 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA 4261 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG 4321 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA 4381 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC 4441 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT 4501 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT 4561 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA 4621 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG 4681 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT 4741 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC 4801 GGATAACAAT TTCACACAGG AAACAGCT // 1517 LOCUS ADDGENE_1517 8407 bp DNA circular SYN 01/03/2005 DEFINITION L2681 complete plasmid sequence KEYWORDS . SOURCE L2681 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1517 at www.addgene.org FEATURES Location/Qualifiers source 1..8407 /organism="Addgene plasmid L2681" /mol_type="other DNA" misc_feature complement(132..156) /label="hsp16-41.5'" misc_feature 370..394 /label="hsp16-2.5'" misc_feature 480..521 /label="SynIVS.A" misc_feature 567..593 /label="SV40.NLS" CDS 567..2162 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf2" /translation="MTAPKKKRKVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVS GEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCF SRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMIL LTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP NEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQKQHLELSRDIAQRFNALYGEIDPV VLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRR*V*TDPY*LTCSDI IFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQVSL IKLILTNKI*LIFRTVVCRLNLT" gene 609..1469 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 780..830 /label="SynIVS.R" misc_feature 852..871 /label="gfp.S65C" misc_feature 985..1035 /label="SynIVS.S" misc_feature 1200..1250 /label="SynIVS.T" gene 1566..5119 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1700..1750 /label="SynIVS.B" CDS 2201..5122 /label="Orf1" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2378..2428 /label="SynIVS.D" misc_feature 2819..2869 /label="SynIVS.E" misc_feature 3126..3176 /label="SynIVS.F" misc_feature 3407..3457 /label="SynIVS.G" misc_feature 3694..3744 /label="SynIVS.H" misc_feature 4060..4110 /label="SynIVS.I" misc_feature 4371..4421 /label="SynIVS.J" misc_feature 4777..4827 /label="SynIVS.K" misc_feature 5143..5193 /label="SynIVS.L" promoter 6321..6349 /label="Amp_promoter" CDS 6391..7251 /label="Ampicillin" /gene="Ampicillin" /note="Orf0" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6391..7251 /label="Ampicillin" /gene="Ampicillin" rep_origin 7406..8025 /label="pBR322_origin" misc_feature 8241..8267 /label="lacI.C" promoter 8334..8363 /label="lac_promoter" misc_feature 8398..8416 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA AACTATAATC ATctcactgG ATCCCCGGGA TTGGCCAAAG GACCCAAAGG 481 TATGTTTCGA ATGATACTAA CATAACATAG AACATTTTCA GGAGGACCCT TGGctagagg 541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaCCGG 601 TAGAAAAAAT GAGTAAAGGA GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT 661 TAGATGGTGA TGTTAATGGG CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA 721 CATACGGAAA ACTTACCCTT AAATTTATTT GCACTACTGG AAAACTACCT GTTCCATGGg 781 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag CCAACACTTG 841 TCACTACTTT CTgTTATGGT GTTCAATGCT TcTCgAGATA CCCAGATCAT ATGAAACgGC 901 ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT ATATTTTTCA 961 AAGATGACGG GAACTACAAG ACACgtaagt ttaaacagtt cggtactaac taaccataca 1021 tatttaaatt ttcagGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG 1081 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAATACA 1141 ACTATAACTC ACACAATGTA TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTg 1201 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag AACTTCAAAA 1261 TTAGACACAA CATTGAAGAT GGAAGCGTTC AACTAGCAGA CCATTATCAA CAAAATACTC 1321 CAATTGGCGA TGGCCCTGTC CTTTTACCAG ACAACCATTA CCTGTCCACA CAATCTGCCC 1381 TTTCGAAAGA TCCCAACGAA AAGAGAGACC ACATGGTCCT TCTTGAGTTT GTAACAGCTG 1441 CTGGGATTAC ACATGGCATG GATGAACTAT ACAAATgcCC GGTGGGTGAA GACCAGAAAC 1501 AGCACCTCGA ACTGAGCCGC GATATTGCCC AGCGTTTCAA CGCGCTGTAT GGCGAGATCG 1561 ATCCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC 1621 TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC 1681 CTTCCCAACA GTTGCGaAGg taagtttaaa cagatccata ctaactaact tgttctgaca 1741 taattttcag CtTGAATGGC GAATGGCGCT TTGCCTGGTT TCCGGCACCA GAAGCGGTGC 1801 CGGAAAGCTG GCTGGAGTGC GATCTTCCTG AGGCCGATAC TGTCGTCGTC CCCTCAAACT 1861 GGCAGATGCA CGGTTACGAT GCGCCCATCT ACACCAACGT GACCTATCCC ATTACGGTCA 1921 ATCCGCCGTT TGTTCCCACG GAGAATCCGA CGGGTTGTTA CTCGCTCACA TTTAATGTTG 1981 ATGAAAGCTG GCTACAGGAA GGCCAGACGC GAATTATTTT TGATGGCGTT AACTCGGCGT 2041 TTCATCTGTG GTGCAACGGG CGCTGGGTCG GTTACGGCCA Ggtaagttta attaagttga 2101 taCTAACTAA CAAGATCTGA TTAATTTTCa gGACAGTCGT TTGCCGTCTG AATTTGACCT 2161 GAGCGCATTT TTACGCGCCG GAGAAAACCG CCTCGCGGTG ATGGTGCTGC GCTGGAGTGA 2221 CGGCAGTTAT CTGGAAGATC AGGATATGTG GCGGATGAGC GGCATTTTCC GTGACGTCTC 2281 GTTGCTGCAT AAACCGACTA CACAAATCAG CGATTTCCAT GTTGCCACTC GCTTTAATGA 2341 TGATTTCtcc CGCGCTGTAC TGGAGGCTGA AGTcCAGgta agtttaaaca ggatcttact 2401 aactaacatg ctaacactga attttcagAT GTGCGGCGAG TTGCGTGACT ACCTACGGGT 2461 AACAGTTTCT TTATGGCAGG GTGAAACGCA GGTCGCCAGC GGCACCGCGC CTTTCGGCGG 2521 TGAAATTATC GATGAGCGTG GTGGTTATGC CGATCGCGTC ACACTACGTC TGAACGTCGA 2581 AAACCCGAAA CTGTGGAGCG CCGAAATCCC GAATCTCTAT CGTGCGGTGG TTGAACTGCA 2641 CACCGCCGAC GGCACGCTGA TTGAAGCAGA AGCCTGCGAT GTCGGTTTCC GCGAGGTGCG 2701 GATTGAAAAT GGTCTGCTGC TGCTGAACGG CAAGCCGTTG CTGATTCGAG GCGTTAACCG 2761 TCACGAGCAT CATCCTCTGC ATGGTCAGGT CATGGATGAG CAGACGATGG TGCAGGATgt 2821 aagtttaaac tattcgttac taactaactt taaacattta aattttcagA TCCTGCTGAT 2881 GAAGCAGAAC AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC CGCTGTGGTA 2941 CACGCTGTGC GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG AAACCCACGG 3001 CATGGTGCCA ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA TGAGCGAACG 3061 CGTAACGCGA ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT GGTCGCTGGG 3121 GAATGgtaag tttaaacagt tgaatactaa ctaacggaga tctttgaaat tttcagAATC 3181 AGGCCACGGC GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG 3241 CCCGGTGCAG TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT 3301 GTACGCGCGC GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA 3361 ATGGCTTTCG CTACCTGGAG AGACGCGCCC GCTGATtCTT TGCGAGgtaa gtttaaacag 3421 aactctacta actaacacat tagatcctaa ttttcagTAC GCtCACGCGA TGGGcAACAG 3481 TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG 3541 CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG 3601 GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG 3661 TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCgtaagtt taaacaataa cctactaact 3721 aacgtagata atttaaattt tcagGCTGAC GGAAGCAAAA CACCAGCAGC AGTTTTTCCA 3781 GTTCCGTTTA TCCGGGCAAA CCATCGAAGT GACCAGCGAA TACCTGTTCC GTCATAGCGA 3841 TAACGAGCTC CTGCACTGGA TGGTGGCGCT GGATGGTAAG CCGCTGGCAA GCGGTGAAGT 3901 GCCTCTGGAT GTCGCTCCAC AAGGTAAACA GTTGATTGAA CTGCCTGAAC TACCGCAGCC 3961 GGAGAGCGCC GGGCAACTCT GGCTCACAGT ACGCGTAGTG CAACCGAACG CGACCGCATG 4021 GTCAGAAGCC GGGCACATCA GCGCaTGGCA GCAGTGGAGg taagtttaaa caagatccta 4081 ctaactaact ctacattgat gaattttcag ACTGGCGGAA AACCTCAGTG TGACGCTCCC 4141 CGCCGCGTCC CACGCCATCC CGCATCTGAC CACCAGCGAA ATGGATTTTT GCATCGAGCT 4201 GGGTAATAAG CGTTGGCAAT TTAACCGCCA GTCAGGCTTT CTTTCACAGA TGTGGATTGG 4261 CGATAAAAAA CAACTGCTGA CGCCGCTGCG CGATCAGTTC ACCCGTGCAC CGCTGGATAA 4321 CGACATTGGC GTAAGTGAAG CGACCCGCAT TGACCCTAAC GCCTGGGTCG gtaagtttaa 4381 acaaagttgt actaactaac gaagatcttg ataattttca gAACGCTGGA AGGCGGCGGG 4441 CCATTACCAG GCCGAAGCAG CGTTGTTGCA GTGCACGGCA GATACACTTG CTGATGCGGT 4501 GCTGATTACG ACCGCTCACG CGTGGCAGCA TCAGGGGAAA ACCTTATTTA TCAGCCGGAA 4561 AACCTACCGG ATTGATGGTA GTGGTCAAAT GGCGATTACC GTTGATGTTG AAGTGGCGAG 4621 CGATACACCG CATCCGGCGC GGATTGGCCT GAACTGCCAG CTGGCGCAGG TAGCAGAGCG 4681 GGTAAACTGG CTCGGATTAG GGCCGCAAGA AAACTATCCC GACCGCCTTA CTGCCGCCTG 4741 TTTTGACCGC TGGGATCTGC CATTGTCAGA CATGTAgtaa gtttaaactt gatagtacta 4801 actaacatgt ttcatttaaa ttttcagTAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT 4861 GCGCTGCGGG ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT 4921 CAACATCAGC CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA 4981 CGCGGAAGAA GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA 5041 CTCCTGGAGC CCGTCAGTAT CGGCGGAATT CCAACTGAGC GCCGGTCGCT ACCATTACCA 5101 ACTTGTCTGG TGTCAAAAAT AATAGGGGCC GCTGTCATCA GAgtaagttt aaactgagtt 5161 ctactaacta acgagtaata tttaaatttt cagCATCTCG CGCCCGTGCC TCTGACTTCT 5221 AAGTCCAATT ACTCTTCAAC ATCCCTACAT GCTCTTTCTC CCTGTGCTCC CACCCCCTAT 5281 TTTTGTTATT ATCAAAAAAC TTCTCTTAAT TTCTTTGTTT TTTAGCTTCT TTTAAGTCAC 5341 CTCTAACAAT GAAATTGTGT AGATTCAAAA ATAGAATTAA TTCGTAATAA AAAGTCGAAA 5401 AAAATTGTGC TCCCTCCCCC CATTAATAAT AATTCTATCC CAAAATCTAC ACAATGTTCT 5461 GTGTACACTT CTTATGTTTT TTACTTCTGA TAAATTTTTT TGAAACATCA TAGAAAAAAC 5521 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT 5581 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA 5641 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT 5701 TGGGGTTTCC CCTATTGTTT GTCAAGATTT CGAGGACGGC GTTTTTCTTG CTAAAATCAC 5761 AAGTATTGAT GAGCACGATG CAAGAAAGAT CGGAAGAAGG TTTGGGTTTG AGGCTCAGTG 5821 GAAGGTGAGT AGAAGTTGAT AATTTGAAAG TGGAGTAGTG TCTATGGGGT TTTTGCCTTA 5881 AATGACAGAA TACATTCCCA ATATACCAAA CATAACTGTT TCCTACTAGT CGGCCGTACG 5941 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 6001 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 6061 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 6121 TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 6181 GCATCAGGCG GCCTTAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA 6241 ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 6301 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 6361 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 6421 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 6481 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 6541 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 6601 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 6661 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 6721 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 6781 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 6841 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 6901 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 6961 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 7021 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 7081 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 7141 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 7201 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 7261 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 7321 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 7381 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 7441 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG 7501 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA 7561 AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 7621 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG 7681 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA 7741 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC 7801 CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT 7861 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 7921 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA 7981 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 8041 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 8101 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 8161 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 8221 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC 8281 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC 8341 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA 8401 AACAGCT // 1518 LOCUS ADDGENE_1518 7532 bp DNA circular SYN 01/03/2005 DEFINITION L2682 complete plasmid sequence KEYWORDS . SOURCE L2682 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1518 at www.addgene.org FEATURES Location/Qualifiers source 1..7532 /organism="Addgene plasmid L2682" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..1287 /label="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVGEDQKQHLE LSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS QQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSN WQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVN SAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLNLT" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 691..4244 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 825..875 /label="SynIVS.B" CDS 1326..4247 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 1503..1553 /label="SynIVS.D" misc_feature 1944..1994 /label="SynIVS.E" misc_feature 2251..2301 /label="SynIVS.F" misc_feature 2532..2582 /label="SynIVS.G" misc_feature 2819..2869 /label="SynIVS.H" misc_feature 3185..3235 /label="SynIVS.I" misc_feature 3496..3546 /label="SynIVS.J" misc_feature 3902..3952 /label="SynIVS.K" misc_feature 4268..4318 /label="SynIVS.L" promoter 5446..5474 /label="Amp_promoter" CDS 5516..6376 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 5516..6376 /label="Ampicillin" /gene="Ampicillin" rep_origin 6531..7150 /label="pBR322_origin" misc_feature 7366..7392 /label="lacI.C" promoter 7459..7488 /label="lac_promoter" misc_feature 7523..7541 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaCCGGTGG GTGAAGACCA GAAACAGCAC CTCGAACTGA GCCGCGATAT TGCCCAGCGT 661 TTCAACGCGC TGTATGGCGA GATCGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC 721 CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 781 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GaAGgtaagt ttaaacagat 841 ccatactaac taacttgttc tgacataatt ttcagCtTGA ATGGCGAATG GCGCTTTGCC 901 TGGTTTCCGG CACCAGAAGC GGTGCCGGAA AGCTGGCTGG AGTGCGATCT TCCTGAGGCC 961 GATACTGTCG TCGTCCCCTC AAACTGGCAG ATGCACGGTT ACGATGCGCC CATCTACACC 1021 AACGTGACCT ATCCCATTAC GGTCAATCCG CCGTTTGTTC CCACGGAGAA TCCGACGGGT 1081 TGTTACTCGC TCACATTTAA TGTTGATGAA AGCTGGCTAC AGGAAGGCCA GACGCGAATT 1141 ATTTTTGATG GCGTTAACTC GGCGTTTCAT CTGTGGTGCA ACGGGCGCTG GGTCGGTTAC 1201 GGCCAGgtaa gtttaattaa gttgataCTA ACTAACAAGA TCTGATTAAT TTTCagGACA 1261 GTCGTTTGCC GTCTGAATTT GACCTGAGCG CATTTTTACG CGCCGGAGAA AACCGCCTCG 1321 CGGTGATGGT GCTGCGCTGG AGTGACGGCA GTTATCTGGA AGATCAGGAT ATGTGGCGGA 1381 TGAGCGGCAT TTTCCGTGAC GTCTCGTTGC TGCATAAACC GACTACACAA ATCAGCGATT 1441 TCCATGTTGC CACTCGCTTT AATGATGATT TCtccCGCGC TGTACTGGAG GCTGAAGTcC 1501 AGgtaagttt aaacaggatc ttactaacta acatgctaac actgaatttt cagATGTGCG 1561 GCGAGTTGCG TGACTACCTA CGGGTAACAG TTTCTTTATG GCAGGGTGAA ACGCAGGTCG 1621 CCAGCGGCAC CGCGCCTTTC GGCGGTGAAA TTATCGATGA GCGTGGTGGT TATGCCGATC 1681 GCGTCACACT ACGTCTGAAC GTCGAAAACC CGAAACTGTG GAGCGCCGAA ATCCCGAATC 1741 TCTATCGTGC GGTGGTTGAA CTGCACACCG CCGACGGCAC GCTGATTGAA GCAGAAGCCT 1801 GCGATGTCGG TTTCCGCGAG GTGCGGATTG AAAATGGTCT GCTGCTGCTG AACGGCAAGC 1861 CGTTGCTGAT TCGAGGCGTT AACCGTCACG AGCATCATCC TCTGCATGGT CAGGTCATGG 1921 ATGAGCAGAC GATGGTGCAG GATgtaagtt taaactattc gttactaact aactttaaac 1981 atttaaattt tcagATCCTG CTGATGAAGC AGAACAACTT TAACGCCGTG CGCTGTTCGC 2041 ATTATCCGAA CCATCCGCTG TGGTACACGC TGTGCGACCG CTACGGCCTG TATGTGGTGG 2101 ATGAAGCCAA TATTGAAACC CACGGCATGG TGCCAATGAA TCGTCTGACC GATGATCCGC 2161 GCTGGCTACC GGCGATGAGC GAACGCGTAA CGCGAATGGT GCAGCGCGAT CGTAATCACC 2221 CGAGTGTGAT CATCTGGTCG CTGGGGAATG gtaagtttaa acagttgaat actaactaac 2281 ggagatcttt gaaattttca gAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT 2341 GGATCAAATC TGTCGATCCT TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA 2401 CGGCCACCGA TATTATTTGC CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG 2461 CTGTGCCGAA ATGGTCCATC AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA 2521 TtCTTTGCGA Ggtaagttta aacagaactc tactaactaa cacattagat cctaattttc 2581 agTACGCtCA CGCGATGGGc AACAGTCTTG GCGGTTTCGC TAAATACTGG CAGGCGTTTC 2641 GTCAGTATCC CCGTTTACAG GGCGGCTTCG TCTGGGACTG GGTGGATCAG TCGCTGATTA 2701 AATATGATGA AAACGGCAAC CCGTGGTCGG CTTACGGCGG TGATTTTGGC GATACGCCGA 2761 ACGATCGCCA GTTCTGTATG AACGGTCTGG TCTTTGCCGA CCGCACGCCG CATCCAGCgt 2821 aagtttaaac aataacctac taactaacgt agataattta aattttcagG CTGACGGAAG 2881 CAAAACACCA GCAGCAGTTT TTCCAGTTCC GTTTATCCGG GCAAACCATC GAAGTGACCA 2941 GCGAATACCT GTTCCGTCAT AGCGATAACG AGCTCCTGCA CTGGATGGTG GCGCTGGATG 3001 GTAAGCCGCT GGCAAGCGGT GAAGTGCCTC TGGATGTCGC TCCACAAGGT AAACAGTTGA 3061 TTGAACTGCC TGAACTACCG CAGCCGGAGA GCGCCGGGCA ACTCTGGCTC ACAGTACGCG 3121 TAGTGCAACC GAACGCGACC GCATGGTCAG AAGCCGGGCA CATCAGCGCa TGGCAGCAGT 3181 GGAGgtaagt ttaaacaaga tcctactaac taactctaca ttgatgaatt ttcagACTGG 3241 CGGAAAACCT CAGTGTGACG CTCCCCGCCG CGTCCCACGC CATCCCGCAT CTGACCACCA 3301 GCGAAATGGA TTTTTGCATC GAGCTGGGTA ATAAGCGTTG GCAATTTAAC CGCCAGTCAG 3361 GCTTTCTTTC ACAGATGTGG ATTGGCGATA AAAAACAACT GCTGACGCCG CTGCGCGATC 3421 AGTTCACCCG TGCACCGCTG GATAACGACA TTGGCGTAAG TGAAGCGACC CGCATTGACC 3481 CTAACGCCTG GGTCGgtaag tttaaacaaa gttgtactaa ctaacgaaga tcttgataat 3541 tttcagAACG CTGGAAGGCG GCGGGCCATT ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA 3601 CGGCAGATAC ACTTGCTGAT GCGGTGCTGA TTACGACCGC TCACGCGTGG CAGCATCAGG 3661 GGAAAACCTT ATTTATCAGC CGGAAAACCT ACCGGATTGA TGGTAGTGGT CAAATGGCGA 3721 TTACCGTTGA TGTTGAAGTG GCGAGCGATA CACCGCATCC GGCGCGGATT GGCCTGAACT 3781 GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA ACTGGCTCGG ATTAGGGCCG CAAGAAAACT 3841 ATCCCGACCG CCTTACTGCC GCCTGTTTTG ACCGCTGGGA TCTGCCATTG TCAGACATGT 3901 Agtaagttta aacttgatag tactaactaa catgtttcat ttaaattttc agTACCCCGT 3961 ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG CGAATTGAAT TATGGCCCAC 4021 ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA CAGTCAACAG CAACTGATGG 4081 AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC ATGGCTGAAT ATCGACGGTT 4141 TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC AGTATCGGCG GAATTCCAAC 4201 TGAGCGCCGG TCGCTACCAT TACCAACTTG TCTGGTGTCA AAAATAATAG GGGCCGCTGT 4261 CATCAGAgta agtttaaact gagttctact aactaacgag taatatttaa attttcagCA 4321 TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT 4381 TTCTCCCTGT GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT 4441 TGTTTTTTAG CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA 4501 ATTAATTCGT AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC 4561 TATCCCAAAA TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT 4621 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 4681 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 4741 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 4801 AATTAATTTT CCTGCTTTTG CTTTTTGGGG TTTCCCCTAT TGTTTGTCAA GATTTCGAGG 4861 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 4921 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 4981 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 5041 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 5101 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 5161 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 5221 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 5281 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 5341 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 5401 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 5461 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 5521 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 5581 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 5641 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 5701 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 5761 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 5821 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 5881 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 5941 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 6001 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 6061 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 6121 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 6181 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 6241 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 6301 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 6361 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 6421 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 6481 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 6541 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 6601 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 6661 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 6721 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 6781 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 6841 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 6901 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 6961 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 7021 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 7081 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 7141 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 7201 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 7261 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 7321 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 7381 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 7441 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 7501 GAGCGGATAA CAATTTCACA CAGGAAACAG CT // 1519 LOCUS ADDGENE_1519 4835 bp DNA circular SYN 01/03/2005 DEFINITION L2684 complete plasmid sequence KEYWORDS . SOURCE L2684 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1519 at www.addgene.org FEATURES Location/Qualifiers source 1..4835 /organism="Addgene plasmid L2684" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..1485 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVEKMSKGEEL FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYIL TNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY KTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNS HNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPI GDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 616..1476 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 787..837 /label="SynIVS.R" misc_feature 859..878 /label="gfp.S65C" misc_feature 992..1042 /label="SynIVS.S" misc_feature 1207..1257 /label="SynIVS.T" misc_feature 1571..1621 /label="SynIVS.L" promoter 2749..2777 /label="Amp_promoter" CDS 2819..3679 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 2819..3679 /label="Ampicillin" /gene="Ampicillin" rep_origin 3834..4453 /label="pBR322_origin" misc_feature 4669..4695 /label="lacI.C" promoter 4762..4791 /label="lac_promoter" misc_feature 4826..4844 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaCCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT 661 GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT 721 GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT 781 CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA 841 ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC AGATCATATG 901 AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA 961 TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa 1021 ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 1081 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 1141 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC 1201 AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC 1261 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA 1321 AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA 1381 TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA 1441 ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATAGCATTC GTAGAATTCC 1501 AACTGAGCGC CGGTCGCTAC CATTACCAAC TTGTCTGGTG TCAAAAATAA TAGGGGCCGC 1561 TGTCATCAGA gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca 1621 gCATCTCGCG CCCGTGCCTC TGACTTCTAA GTCCAATTAC TCTTCAACAT CCCTACATGC 1681 TCTTTCTCCC TGTGCTCCCA CCCCCTATTT TTGTTATTAT CAAAAAACTT CTCTTAATTT 1741 CTTTGTTTTT TAGCTTCTTT TAAGTCACCT CTAACAATGA AATTGTGTAG ATTCAAAAAT 1801 AGAATTAATT CGTAATAAAA AGTCGAAAAA AATTGTGCTC CCTCCCCCCA TTAATAATAA 1861 TTCTATCCCA AAATCTACAC AATGTTCTGT GTACACTTCT TATGTTTTTT ACTTCTGATA 1921 AATTTTTTTG AAACATCATA GAAAAAACCG CACACAAAAT ACCTTATCAT ATGTTACGTT 1981 TCAGTTTATG ACCGCAATTT TTATTTCTTC GCACGTCTGG GCCTCTCATG ACGTCAAATC 2041 ATGCTCATCG TGAAAAAGTT TTGGAGTATT TTTGGAATTT TTCAATCAAG TGAAAGTTTA 2101 TGAAATTAAT TTTCCTGCTT TTGCTTTTTG GGGTTTCCCC TATTGTTTGT CAAGATTTCG 2161 AGGACGGCGT TTTTCTTGCT AAAATCACAA GTATTGATGA GCACGATGCA AGAAAGATCG 2221 GAAGAAGGTT TGGGTTTGAG GCTCAGTGGA AGGTGAGTAG AAGTTGATAA TTTGAAAGTG 2281 GAGTAGTGTC TATGGGGTTT TTGCCTTAAA TGACAGAATA CATTCCCAAT ATACCAAACA 2341 TAACTGTTTC CTACTAGTCG GCCGTACGGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC 2401 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 2461 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG 2521 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA 2581 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGGC CTTAAGGGCC TCGTGATACG 2641 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT 2701 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 2761 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT 2821 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT 2881 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG 2941 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA 3001 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG 3061 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT 3121 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG 3181 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG 3241 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA 3301 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC 3361 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC 3421 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC 3481 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG 3541 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC 3601 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC 3661 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT 3721 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC 3781 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3841 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3901 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3961 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG 4021 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 4081 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 4141 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 4201 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT 4261 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 4321 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 4381 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 4441 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 4501 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 4561 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 4621 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 4681 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT 4741 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT 4801 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCT // 1520 LOCUS ADDGENE_1520 8414 bp DNA circular SYN 01/03/2005 DEFINITION L2685 complete plasmid sequence KEYWORDS . SOURCE L2685 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1520 at www.addgene.org FEATURES Location/Qualifiers source 1..8414 /organism="Addgene plasmid L2685" /mol_type="other DNA" misc_feature complement(147..171) /label="hsp16-2.5'" CDS 331..2169 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MHLGPSRTFCKAAECGYIRKAGSEEANTLCSSASKKLRKSSSDP RDWPKDPKVCFE*Y*HNIEHFQEDPWLEDPRVPSSEKMTAPKKKRKVPVEKMSKGEEL FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWVSLNIYIL TNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY KTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNS HNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKIRHNIEDGSVQLADHYQQNTPI GDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKCPVGEDQK QHLELSRDIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRR*V*TDPY*LTCSDIIFSLNGEWRFAWFPAPEAVPESWLECDLPEADTVV VPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIF DGVNSAFHLWCNGRWVGYGQVSLIKLILTNKI*LIFRTVVCRLNLT" misc_feature 385..409 /label="hsp16-41.5'" misc_feature 487..528 /label="SynIVS.A" misc_feature 574..600 /label="SV40.NLS" gene 616..1476 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 787..837 /label="SynIVS.R" misc_feature 859..878 /label="gfp.S65C" misc_feature 992..1042 /label="SynIVS.S" misc_feature 1207..1257 /label="SynIVS.T" gene 1573..5126 /label="lacZ(w/ gaps)" /gene="lacZ(w/ gaps)" misc_feature 1707..1757 /label="SynIVS.B" CDS 2208..5129 /label="Orf2" /translation="MVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQVSLNRILLTNMLTLNFQMCGELRDYLRVTVSLWQGETQVASGT APFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACD VGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDVSLNYSLLTNFK HLNFQILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTD DPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNGKFKQLNTN*RRSLKFSESGHGANHD ALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLP GETRPLILCEVSLNRTLLTNTLDPNFQYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVW DWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPA*V*TITY*LT *II*IFRLTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGE VPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWR*V*T RSY*LTLH**IFRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLS QMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVGKFKQSCTN*RRS**FS ERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMA ITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLS DM**V*T**Y*LTCFI*IFSTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQ QQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQ K" misc_feature 2385..2435 /label="SynIVS.D" misc_feature 2826..2876 /label="SynIVS.E" misc_feature 3133..3183 /label="SynIVS.F" misc_feature 3414..3464 /label="SynIVS.G" misc_feature 3701..3751 /label="SynIVS.H" misc_feature 4067..4117 /label="SynIVS.I" misc_feature 4378..4428 /label="SynIVS.J" misc_feature 4784..4834 /label="SynIVS.K" misc_feature 5150..5200 /label="SynIVS.L" promoter 6328..6356 /label="Amp_promoter" CDS 6398..7258 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 6398..7258 /label="Ampicillin" /gene="Ampicillin" rep_origin 7413..8032 /label="pBR322_origin" misc_feature 8248..8274 /label="lacI.C" promoter 8341..8370 /label="lac_promoter" misc_feature 8405..8423 /label="M13r_primer" ORIGIN 1 ATGACCATGA TTACGCCAag cttgcatgcc tgcaggtcga ctctagagGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATTTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTAGaggatc cccgggtacc gagctcagaa aaaatgactg ctccaaagaa gaagcgtaag 601 gtaCCGGTAG AAAAAATGAG TAAAGGAGAA GAACTTTTCA CTGGAGTTGT CCCAATTCTT 661 GTTGAATTAG ATGGTGATGT TAATGGGCAC AAATTTTCTG TCAGTGGAGA GGGTGAAGGT 721 GATGCAACAT ACGGAAAACT TACCCTTAAA TTTATTTGCA CTACTGGAAA ACTACCTGTT 781 CCATGGgtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcagCCA 841 ACACTTGTCA CTACTTTCTg TTATGGTGTT CAATGCTTcT CgAGATACCC AGATCATATG 901 AAACgGCATG ACTTTTTCAA GAGTGCCATG CCCGAAGGTT ATGTACAGGA AAGAACTATA 961 TTTTTCAAAG ATGACGGGAA CTACAAGACA Cgtaagttta aacagttcgg tactaactaa 1021 ccatacatat ttaaattttc agGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT 1081 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG 1141 GAATACAACT ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC 1201 AAAGTTgtaa gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagAAC 1261 TTCAAAATTA GACACAACAT TGAAGATGGA AGCGTTCAAC TAGCAGACCA TTATCAACAA 1321 AATACTCCAA TTGGCGATGG CCCTGTCCTT TTACCAGACA ACCATTACCT GTCCACACAA 1381 TCTGCCCTTT CGAAAGATCC CAACGAAAAG AGAGACCACA TGGTCCTTCT TGAGTTTGTA 1441 ACAGCTGCTG GGATTACACA TGGCATGGAT GAACTATACA AATgcCCGGT GGGTGAAGAC 1501 CAGAAACAGC ACCTCGAACT GAGCCGCGAT ATTGCCCAGC GTTTCAACGC GCTGTATGGC 1561 GAGATCGATC CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 1621 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC 1681 GATCGCCCTT CCCAACAGTT GCGaAGgtaa gtttaaacag atccatacta actaacttgt 1741 tctgacataa ttttcagCtT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC GGCACCAGAA 1801 GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT CGTCGTCCCC 1861 TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC CTATCCCATT 1921 ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC GCTCACATTT 1981 AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA TGGCGTTAAC 2041 TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGgt aagtttaatt 2101 aagttgataC TAACTAACAA GATCTGATTA ATTTTCagGA CAGTCGTTTG CCGTCTGAAT 2161 TTGACCTGAG CGCATTTTTA CGCGCCGGAG AAAACCGCCT CGCGGTGATG GTGCTGCGCT 2221 GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG GATGAGCGGC ATTTTCCGTG 2281 ACGTCTCGTT GCTGCATAAA CCGACTACAC AAATCAGCGA TTTCCATGTT GCCACTCGCT 2341 TTAATGATGA TTTCtccCGC GCTGTACTGG AGGCTGAAGT cCAGgtaagt ttaaacagga 2401 tcttactaac taacatgcta acactgaatt ttcagATGTG CGGCGAGTTG CGTGACTACC 2461 TACGGGTAAC AGTTTCTTTA TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT 2521 TCGGCGGTGA AATTATCGAT GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA 2581 ACGTCGAAAA CCCGAAACTG TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG 2641 AACTGCACAC CGCCGACGGC ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG 2701 AGGTGCGGAT TGAAAATGGT CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG 2761 TTAACCGTCA CGAGCATCAT CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC 2821 AGGATgtaag tttaaactat tcgttactaa ctaactttaa acatttaaat tttcagATCC 2881 TGCTGATGAA GCAGAACAAC TTTAACGCCG TGCGCTGTTC GCATTATCCG AACCATCCGC 2941 TGTGGTACAC GCTGTGCGAC CGCTACGGCC TGTATGTGGT GGATGAAGCC AATATTGAAA 3001 CCCACGGCAT GGTGCCAATG AATCGTCTGA CCGATGATCC GCGCTGGCTA CCGGCGATGA 3061 GCGAACGCGT AACGCGAATG GTGCAGCGCG ATCGTAATCA CCCGAGTGTG ATCATCTGGT 3121 CGCTGGGGAA TGgtaagttt aaacagttga atactaacta acggagatct ttgaaatttt 3181 cagAATCAGG CCACGGCGCT AATCACGACG CGCTGTATCG CTGGATCAAA TCTGTCGATC 3241 CTTCCCGCCC GGTGCAGTAT GAAGGCGGCG GAGCCGACAC CACGGCCACC GATATTATTT 3301 GCCCGATGTA CGCGCGCGTG GATGAAGACC AGCCCTTCCC GGCTGTGCCG AAATGGTCCA 3361 TCAAAAAATG GCTTTCGCTA CCTGGAGAGA CGCGCCCGCT GATtCTTTGC GAGgtaagtt 3421 taaacagaac tctactaact aacacattag atcctaattt tcagTACGCt CACGCGATGG 3481 GcAACAGTCT TGGCGGTTTC GCTAAATACT GGCAGGCGTT TCGTCAGTAT CCCCGTTTAC 3541 AGGGCGGCTT CGTCTGGGAC TGGGTGGATC AGTCGCTGAT TAAATATGAT GAAAACGGCA 3601 ACCCGTGGTC GGCTTACGGC GGTGATTTTG GCGATACGCC GAACGATCGC CAGTTCTGTA 3661 TGAACGGTCT GGTCTTTGCC GACCGCACGC CGCATCCAGC gtaagtttaa acaataacct 3721 actaactaac gtagataatt taaattttca gGCTGACGGA AGCAAAACAC CAGCAGCAGT 3781 TTTTCCAGTT CCGTTTATCC GGGCAAACCA TCGAAGTGAC CAGCGAATAC CTGTTCCGTC 3841 ATAGCGATAA CGAGCTCCTG CACTGGATGG TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG 3901 GTGAAGTGCC TCTGGATGTC GCTCCACAAG GTAAACAGTT GATTGAACTG CCTGAACTAC 3961 CGCAGCCGGA GAGCGCCGGG CAACTCTGGC TCACAGTACG CGTAGTGCAA CCGAACGCGA 4021 CCGCATGGTC AGAAGCCGGG CACATCAGCG CaTGGCAGCA GTGGAGgtaa gtttaaacaa 4081 gatcctacta actaactcta cattgatgaa ttttcagACT GGCGGAAAAC CTCAGTGTGA 4141 CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA 4201 TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT 4261 GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC 4321 TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGgta 4381 agtttaaaca aagttgtact aactaacgaa gatcttgata attttcagAA CGCTGGAAGG 4441 CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG 4501 ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA 4561 GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG 4621 TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG 4681 CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG 4741 CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT GTAgtaagtt taaacttgat 4801 agtactaact aacatgtttc atttaaattt tcagTACCCC GTACGTCTTC CCGAGCGAAA 4861 ACGGTCTGCG CTGCGGGACG CGCGAATTGA ATTATGGCCC ACACCAGTGG CGCGGCGACT 4921 TCCAGTTCAA CATCAGCCGC TACAGTCAAC AGCAACTGAT GGAAACCAGC CATCGCCATC 4981 TGCTGCACGC GGAAGAAGGC ACATGGCTGA ATATCGACGG TTTCCATATG GGGATTGGTG 5041 GCGACGACTC CTGGAGCCCG TCAGTATCGG CGGAATTCCA ACTGAGCGCC GGTCGCTACC 5101 ATTACCAACT TGTCTGGTGT CAAAAATAAT AGGGGCCGCT GTCATCAGAg taagtttaaa 5161 ctgagttcta ctaactaacg agtaatattt aaattttcag CATCTCGCGC CCGTGCCTCT 5221 GACTTCTAAG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC 5281 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT 5341 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA 5401 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA 5461 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG 5521 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT 5581 TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA TGCTCATCGT GAAAAAGTTT 5641 TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT GAAATTAATT TTCCTGCTTT 5701 TGCTTTTTGG GGTTTCCCCT ATTGTTTGTC AAGATTTCGA GGACGGCGTT TTTCTTGCTA 5761 AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT GGGTTTGAGG 5821 CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT ATGGGGTTTT 5881 TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC TACTAGTCGG 5941 CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG 6001 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT 6061 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG 6121 CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA 6181 AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT 6241 CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 6301 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 6361 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 6421 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 6481 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 6541 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 6601 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 6661 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 6721 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 6781 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 6841 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 6901 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 6961 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 7021 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 7081 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 7141 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 7201 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 7261 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 7321 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 7381 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 7441 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 7501 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 7561 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 7621 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 7681 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 7741 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 7801 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 7861 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 7921 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 7981 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 8041 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 8101 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 8161 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC 8221 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA 8281 AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC 8341 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA 8401 CACAGGAAAC AGCT // 1521 LOCUS ADDGENE_1521 3963 bp DNA circular SYN 01/03/2005 DEFINITION L2822 complete plasmid sequence KEYWORDS . SOURCE L2822 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1521 at www.addgene.org FEATURES Location/Qualifiers source 1..3963 /organism="Addgene plasmid L2822" /mol_type="other DNA" misc_feature 21..39 /label="M13r_primer" CDS 31..1608 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" /note="Orf0" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPVEKMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWVSLNIYILTNPDYLNFQPTLVTTFCYGVQCFSRYPDHMKRHDFFK SAMPEGYVQERTIFFKDDGNYKTRKFKQFGTN*PYIFKFSGAEVKFEGDTLVNRIELK GIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVVSLNMILLTN*SDLNFQNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVT AAGITHGMDELYKCPGDRWSSTAVAAALELVDPPGCRNSISSLSIPSTSRGGPVPNSP YSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN GEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALNRGLP LGFRFSALRHLDPKKLD" promoter 56..75 /label="T3_promoter" gene 205..1065 /label="GFP(w/ gaps)" /gene="GFP(w/ gaps)" misc_feature 376..426 /label="SynIVS.R" misc_feature 448..467 /label="gfp.S65C" misc_feature 581..631 /label="SynIVS.S" misc_feature 796..846 /label="SynIVS.T" promoter complement(1204..1222) /label="T7_promoter" misc_feature 1242..1390 /label="lacZ_a" misc_feature complement(1233..1249) /label="M13f20_primer" rep_origin 1408..1714 /label="f1_origin" promoter 1907..1935 /label="Amp_promoter" gene 1977..2837 /label="Ampicillin" /gene="Ampicillin" CDS 1977..2837 /label="Ampicillin" /gene="Ampicillin" /note="Orf2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2992..3611 /label="pBR322_origin" misc_feature 3827..3853 /label="lacI.C" promoter 3920..3949 /label="lac_promoter" ORIGIN 1 GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CGCGCAATTA 61 ACCCTCACTA AAGGGAACAA AAGCTGGAGC TCCACCGCGG TGGCGGCCGC TCTAGAACTA 121 GTGGATCCCC CGGGCTGCAG GAATTCGATA TCAAGCTTAT CGATACCGTC GACCTCGAGG 181 GGGGGCCCGG TACCGGTAGA AAAAATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC 241 CCAATTCTTG TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG 301 GGTGAAGGTG ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA 361 CTACCTGTTC CATGGgtaag tttaaacata tatatactaa ctaaccctga ttatttaaat 421 tttcagCCAA CACTTGTCAC TACTTTCTgT TATGGTGTTC AATGCTTcTC gAGATACCCA 481 GATCATATGA AACgGCATGA CTTTTTCAAG AGTGCCATGC CCGAAGGTTA TGTACAGGAA 541 AGAACTATAT TTTTCAAAGA TGACGGGAAC TACAAGACAC gtaagtttaa acagttcggt 601 actaactaac catacatatt taaattttca gGTGCTGAAG TCAAGTTTGA AGGTGATACC 661 CTTGTTAATA GAATCGAGTT AAAAGGTATT GATTTTAAAG AAGATGGAAA CATTCTTGGA 721 CACAAATTGG AATACAACTA TAACTCACAC AATGTATACA TCATGGCAGA CAAACAAAAG 781 AATGGAATCA AAGTTgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat 841 tttcagAACT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT 901 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG 961 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT 1021 GAGTTTGTAA CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA ATgcccgggg 1081 gatcggtgga gctcCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC 1141 AGGAATTCGA TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT 1201 TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC 1261 TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC 1321 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 1381 GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 1441 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 1501 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 1561 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 1621 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 1681 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 1741 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 1801 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAGGT GGCACTTTTC 1861 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 1921 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 1981 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 2041 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 2101 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 2161 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 2221 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 2281 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 2341 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 2401 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 2461 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 2521 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 2581 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 2641 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 2701 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 2761 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 2821 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 2881 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 2941 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 3001 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 3061 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 3121 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 3181 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 3241 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 3301 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 3361 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 3421 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 3481 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 3541 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 3601 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 3661 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 3721 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 3781 GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG 3841 ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA 3901 CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 3961 TGA // 1522 LOCUS ADDGENE_1522 9726 bp DNA circular SYN 01/03/2005 DEFINITION L2865 complete plasmid sequence KEYWORDS . SOURCE L2865 ORGANISM other sequences; artificial sequences; vectors. COMMENT See Addgene plasmid 1522 at www.addgene.org FEATURES Location/Qualifiers source 1..9726 /organism="Addgene plasmid L2865" /mol_type="other DNA" misc_feature 577..600 /label="let858.Tsa" CDS 5381..6085 /label="Orf1" /translation="MIGLGGLTLELREWLAKGLKKKKGMLDQLKAESSSDSSSSSDSS DSSDSSDSDDSSDSSSDSSSSSESEPEPPKKKKKKNSEESSKKKEKENIGRRDRGDKR AERHRDQSVENKDKDRRRRQDSDENRRPERGDDRKDRSKDRRRQDSDDEDRKGRERRE DSGERRRGDRDRRDRNKDQEDHREDRRDRSKDREDRRDRRRHDSDDDRKTRRDRSEER GGRRREVESDDRRRRR" misc_feature 6061..6085 /label="let858.C" misc_feature 6163..6192 /label="let858.3'" promoter complement(6911..6929) /label="T7_promoter" misc_feature 6934..7094 /label="lacZ_a" misc_feature complement(6937..6953) /label="M13f20_primer" rep_origin 7112..7418 /label="f1_origin" promoter 7612..7640 /label="Amp_promoter" gene 7682..8542 /label="Ampicillin" /gene="Ampicillin" CDS 7682..8542 /label="Ampicillin" /gene="Ampicillin" /note="Orf1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 8697..9316 /label="pBR322_origin" misc_feature 9532..9558 /label="lacI.C" misc_feature 9689..9707 /label="M13r_primer" promoter 9724..9743 /label="T3_promoter" ORIGIN 1 TAACCCTCAC TAAAGGGAAC AAAAGCTGGG GGGGATCctc caaaatcgtc ttccgctctg 61 aaaaacgaaa gtggaccttt gacatccgaa aaaatgggcg aaaaaatgaa attgagcttt 121 ttgggtcgaa aaaaatgttt ttagaatgct gagaacacgt taaacacgaa gatcatattt 181 attttgagac ccggatgctc tgaaaatgtc tgacatagat ttaaaaaagc atatatatat 241 ttttcatttt caacgtgaaa gttttgtgca actttataga atctcctatt ggcacattgt 301 tttttattta actgaggcag tttttgaaca cctttttgaa actttgaatc tctttgaagt 361 atactgtcga aaagactgac ttgagcgttc gaaatgccag aagaaaacta tatttgaatc 421 tcgcgctaaa ttgagaaatg caaccgcgct ccactggaca attggaaaaa aaatttattc 481 ggaggcgaca acggtatttt cgaaattgat tttctgtgta ttttctcatt ttttataaat 541 tcttctttga tttatcgttc gtttgtgaga aatttaattg tattcaaact tttttatagT 601 AAGATaccgg tggtaccgct agccgtacga acccgggttc tagaagcgct gctaaggggg 661 cccTCGTCGA GTCGGTCACA ATCACCTGAA ACTCCAAAGG CAGCCAGTGA GGAACGTGAA 721 GAAGAAGAAA AAGAGTCATC TGAACAGgtt tgattttctt tctggtcaaa aagatgaaat 781 tattgatttt cagCCAGATA CTCCCAAAAC TAGCAGCGAG AAGTCTGCAA GTCGTTCACA 841 GTCGCCCAGA GAATCGCGGG AAGTGAGCCA AGAGgtatgt ttttcaaaaa tcaataactg 901 atcataattt ttattgtttg gtgaatttaa gaaaataata ttcgaaaatt cctctgaatt 961 atcaagattg cagtattaat ttcgagaaaa attgagatat tcatagagct attgtaaatt 1021 ttcttgattt cagACTGAAA CTTCGGAAAA TCAAGAGAAA ATCAAAGAAA AGGATGACGG 1081 GGATGATCAG CCTGGCACAC CGAACAGCTA TAGAAGCCGG GAAACTTCAC CAGCTCCAAA 1141 AAGGTCCAAG GAGACCAGgt ttgtcaaaag cttcctgcga ttaattctca tttcaatttt 1201 tcagAGAATC AGAGTCTCCT GAAAAATCCC CGGTTCGTTC AAGATCTCCC AGAAGGTCTT 1261 CAGCACGTTC CCCGTCACGA TCTCCTAGAC GGCGCCGAGA AAGAAGCTCA GAAAGAAAGC 1321 AATCCGAAGA GCCAGCACCG CTACCAGAGA AAAAGAAGAA AGAGCCGCTG GATATTCTAC 1381 GAACAAGAAC CGGAGGAGCA TATATTCCAC CCGCCAAACT TCGACTTATG CAACAACAGA 1441 TTAGTGATAA GCAAAGTGAA CAGTATCAGA GAATGAATTG GGAAAGAATG AAGAAAAAGA 1501 TTCACGGATT GGTTAACAGA GTCAACGCGA AGAATCTTGT TCAAATTGTC AGAGAACTTC 1561 TTCAAGAGAA TGTGATTCGT TCAAAgtgag tgagaaaatc gaaggaaaag gaaagaatta 1621 atttaatttt tcagGGGACT TCTCTGCCGT GACATTATTC AAGCTCAGGC TTTCTCACCA 1681 GGATTCTCTA ACGTCTATGC AGCTTTGGCG GCAGTTATCA ACTCGAAATT CCCTCATGTC 1741 GGTGAACTTC TTCTCCGTCG TCTGATTGTA CAGTTCAAAA GAAGTTTCCG TAGAAATGAC 1801 AGAGGCGTCA CGGTGAACGT GATCAAATTC ATCGCACATT TGATTAATCA ACAAGTTGCT 1861 CACGAAGTTC TTGCGCTGGA AATCATGATT CTGATGCTTG AAGAACCAAC TGATGATTCA 1921 GTTGAAGTCG CCATTGCGTT CCTGAAAGAG TGTGGAGCAA AGCTTCTGGA GATTGCTCCA 1981 GCAGCTCTTA ACAGTGTCTA CGACCGTCTT CGTGCAATTC TCATGGAAAC TGAAAGATCG 2041 GAAAATGCAC TGGATCGACG TATTCAGTAT ATGATTGAGA CTGCAATGCA GATTCGAAAG 2101 GACAAATTTG CGgtaaggta gaatatataa atagtttatt agaaaaaaat aaattagaat 2161 aatttaaatt cctactagcc aatcaggcga cctttttgcg catagttcta ttattgaaaa 2221 atttggagaa tttctcatat tctcgctcgg aaatctggaa ttcgacgaga tcttctggct 2281 tctgtgcagc tgcatcgctt tgtgctccct ttctcgcttg tcttctgtgt acaccaagaa 2341 ccttgttgag ttcatcaact gaatctgtga ctggcttgtt gctcactgga tgcactagac 2401 gactgattct cgagaaatca gattgagttg cgattagggt gacctagaaa ttgggaataa 2461 tacgaacttt tgaaaatatt caggaggatt aaaaaaatta ttctcgacaa tcctacaaat 2521 ttacttattg caccatgttg ctccaacatt tttcattaaa agttaatgaa aaaatgtaga 2581 aaatcggaaa ttggcaattt tcagaccatt tttaagcatt ttcaaaaaaa aattgcagct 2641 gaaataaatg tcattttcag ataaatcgag cgattttctg ttgtctgaca ctagttttta 2701 gttttaaaaa atgttggaag aacatggtgc aataggtaat ttcatagaat ttccatgtgt 2761 tttttttcaa ttaaccaatt atccaaatct tccaaactca cattttgcgg agctgggcta 2821 tcaagaatct gctgcagttt tataagacga gcatctctga tatcactgaa aattaatttt 2881 taatcaaaac ttgaatatca actaaaccca cttattaact ttctcgatct tctgtcgttc 2941 ggtacgatga cggtgaagaa gccaattgta gtagttgatt tggttcaagt cctttcggtg 3001 ttgtacgtca gtgtcctgca atgctattta gttataactt aggcctaaga ttcaatttaa 3061 tgaagtgatt aaatttgttc tctgaacctc ttaagatgat cttttggatt agaaacatat 3121 aagacaggtt tacctatcta ttaaaaaaca gatcaaaata gatacgacca aatcggataa 3181 tccatgccta cctggcatct aggaacgtgt tcttagaaga tttcttacgt aatcgtatga 3241 agaaataaca atttgatcgt tggccagcaa aaatagggtt ttaagtggga tagtgttttt 3301 attagctaac cggaaaattt tatagttttt ttttgcaaga aaccactgaa aaccccctaa 3361 ttgtatacat tttttggagc agcttctggt ctttttgagc aataaaattc gataaaacag 3421 aatttaagtg taaattgttc acatttagtt tctattttat caaattttgt tgctcaaaaa 3481 cattcgaagc tgctctaaaa aaatgcatta aaaaaggggt tttcagtggt ttttcacatt 3541 aaaaaagcta attttaacta aaaatccatc atatttccaa ctttgtcaca acaataaaat 3601 gctggtcaaa atgtgttcga aaaaatgttt ttttttttaa tttttataat ttaaaaatag 3661 ttttctttcg ctgggacaca tacatttttg ggcgtaaatt ttcagttcaa atttccattt 3721 ttacaaccat aatcataaag ctacgtctga tctctctcgc acttacctgc gcctgattcg 3781 aaagaacaac cgtagccaaa agaacaagaa gaacaagcac gtagttgtgg tagtggacgt 3841 tcatcacgca atactgacca atggtcgtgg ggtctcactt tccgtactat tgagagaggg 3901 gagactgaag atggcaattg aggacagtgt cttcgacgca cgcatgcatc cataagcata 3961 atccaggagg gatggagaga aaaatcttgt ttctaagccc ctccctttgt aatacataca 4021 catatctaat accgaagaat ggctaattga atggacgtca gctgttgctg tagttgccaa 4081 ggcatcatcg atgaaataac tgaaagaaag aattaaataa ttattgcagG CGTATCCGGC 4141 GGTCATTGAA GACTTGGACT TGATTGAGGA GGAGGATCAG ATCATCCATA CACTTAATTT 4201 GGAGGATGCG GTTGATCCGG AAAATGGGCT TAgtaagtga ctgaccacac gcggggggca 4261 ttaatttaat aaattgaatt ccatttcagA TGTGTTCAAA CTAGATCCAG AATTCGAAAA 4321 GAACGAGGAG GTTTATGAGG AGATCCGTAA GGAAATCATT GGAAACGCCG ATATTTCGGA 4381 TGAGGATGGT GGCGACGAGT TGGATGATGA AGAAGAGGGT AGTGATGTGG AAGAGGCTCC 4441 GAAGAAGACT ACAGAGATTA TTGATAATAC TGATCAGAAT TGACTGCTTT CAGAAGgtat 4501 tcattttgag ttttgggccg gcaaatctgt aagttgccgg ttgccgaaaa tttgctgaat 4561 ttgccggaaa aaaaaattcc ggaatttatt taaaaacttt ttgtaaaaat taaattaaat 4621 ttgcaacttt tcagAGAAGT CTACCTGACA ATGCAATCAT CTTTGGACTA CCAAGAAGCT 4681 GCTCACAAAT TGCTGAAAAT GAAGATTCCA GACAGCATGC AGgtcagcga tgttgcaaag 4741 aaaaattttc gaccaaaaaa accaaccaat cataaaattt aaaaaaaaac tccgtttttt 4801 tctttttttt tatacgagaa aaaccaaaaa aatgtatttt tgccaaattc taaaatacta 4861 tccccgaaat tttcaatatt ttctctttca gAACGAACTC TGCGCGATGC TTGTCGATTG 4921 TTGTGCTCAA CAGCGTACCT ACGAGCGATT CTACGGAATG CTCATCGAAC GTTTCTGCCG 4981 ACTTCGCCTC GAATACCAGC AATACTTTGA AAAGCTCTGC CAGGACACGT ATTCCACGAT 5041 TCACCGAATT GACATCACAA AACTGCGGAA TTTGGCTCGC CTTATTGCTC ATTTGCTCTC 5101 GACGGATGCT ATTGACTGGA AGATTTTGGC CGATATGAAA ATGACCGAAG AGGACACAAC 5161 TTCTTCTGGC AGAATCTATA TTAAATATAT ATTTAATGAA CTTGTGGAGG CGATGGGAAT 5221 GGTTAAACTT CATTCGAGAG TTACTGATCC gtgagtttcc tagagagagt tgttttcgta 5281 ttcaattttc cctattttca gAACTTTGGC TCATTGCTTT GTTGGATTAT TCCCACGAAC 5341 TAATCCGAAC AGCGCACGAT TTTCGATCAA CTTCTTCACA ATGA