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Human Kinase ORF Kit
(Kit # 1000000014 )

Depositing Labs:   William Hahn, David Root

This kit consists of 558 distinct human kinases and kinase-related protein Open Reading Frames (ORFs) in pDONR-223 Gateway® Entry vectors. All clones are clonal isolates and have been end-read sequenced to confirm identity.

This kit consists of six 96-well plates, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80° Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$1500 USD + shipping

Available to academics and nonprofits only.

Original Publications

These plasmids have been described in:

A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 26;8(8):659-61, DOI : 10.1038/nmeth.1638 Publication Date: 2011 June. - PubMed .

COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA. Nature 16;468(7326):968-72 DOI : 10.1038/nature09627 Publication Date: 2010 Dec. - PubMed .

Description

The Center for Cancer Systems Biology (Dana Farber Cancer Institute)-Broad Human Kinase ORF collection from Addgene consists of 559 distinct human kinases and kinase-related protein ORFs in pDONR-223 Gateway® Entry vectors. All clones are clonal isolates and have been end-read sequenced to confirm identity. For more information see the associated publications.

Kinase ORFs were assembled from a number of sources; 56% were isolated as single cloned isolates from the ORFeome 5.1 collection ( http://horfdb.dfci.harvard.edu ); 31% were cloned from normal human tissue RNA (Ambion) by reverse transcription and subsequent PCR amplification adding Gateway® sequences; 11% were cloned into Entry vectors from templates provided by the Harvard Institute of Proteomics (HIP); 2% additional kinases were cloned into Entry vectors from templates obtained from collaborating laboratories. All ORFs are open (stop codons removed) except for 5 (MST1R, PTK7, JAK3, AXL, TIE1) which are closed (have stop codons).

human-kinase-pie.jpg

Read the interview with DFCI Scientists, William Hahn and Jesse Boehm, who created the kinase libary. Click Here

Kit Documentation

Icon Broad human Kinase Kit info pages.pdf (186.7 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit was a gift from William Hahn and David Root (Addgene kit # 1000000014)"

For your Reference section:
A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 26;8(8):659-61, DOI : 10.1038/nmeth.1638 Publication Date: 2011 June. - PubMed .

COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA. Nature 16;468(7326):968-72 DOI : 10.1038/nature09627 Publication Date: 2010 Dec. - PubMed .

CCSB-Broad Human Kinase ORF Collection - #1000000014

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 pDONR223-CAMK2B
Spectinomycin
Plate 1 / A / 2 pDONR223-NEK3
Spectinomycin
Plate 1 / A / 3 pDONR223-ACVR1
Spectinomycin
Plate 1 / A / 4 pDONR223-NME1-NME2
Spectinomycin
Plate 1 / A / 5 pDONR223-CRKL
Spectinomycin
Plate 1 / A / 6 pDONR223-CSNK1A1
Spectinomycin
Plate 1 / A / 7 pDONR223-CCL4
Spectinomycin
Plate 1 / A / 8 pDONR223-STK33
Spectinomycin
Plate 1 / A / 9 pDONR223-C1orf57
Spectinomycin
Plate 1 / A / 10 pDONR223-CSNK2B
Spectinomycin
Plate 1 / A / 11 pDONR223-PRKAB1
Spectinomycin
Plate 1 / A / 12 pDONR223-LOC389599
Spectinomycin
Plate 1 / B / 1 pDONR223-CAMKK1
Spectinomycin
Plate 1 / B / 2 pDONR223-DYRK4
Spectinomycin
Plate 1 / B / 3 pDONR223-CIB1
Spectinomycin
Plate 1 / B / 4 pDONR223-GK2
Spectinomycin
Plate 1 / B / 5 pDONR223-PRKCI
Spectinomycin
Plate 1 / B / 6 pDONR223-FLJ25006
Spectinomycin
Plate 1 / B / 7 pDONR223-CDK3
Spectinomycin
Plate 1 / B / 8 pDONR223-PION
Spectinomycin
Plate 1 / B / 9 pDONR223-STK19
Spectinomycin
Plate 1 / B / 10 pDONR223-GRK6
Spectinomycin
Plate 1 / B / 11 pDONR223-LOC442075
Spectinomycin
Plate 1 / B / 12 pDONR223-LOC390877
Spectinomycin
Plate 1 / C / 1 pDONR223-AURKC
Spectinomycin
Plate 1 / C / 2 pDONR223-FLJ23356
Spectinomycin
Plate 1 / C / 3 pDONR223-PRKAR1B
Spectinomycin
Plate 1 / C / 4 pDONR223-FLJ40852
Spectinomycin
Plate 1 / C / 5 pDONR223-SGK2
Spectinomycin
Plate 1 / C / 6 pDONR223-TESK1
Spectinomycin
Plate 1 / C / 7 pDONR223-GUK1
Spectinomycin
Plate 1 / C / 8 pDONR223-DCK
Spectinomycin
Plate 1 / C / 9 pDONR223-CDKL4
Spectinomycin
Plate 1 / C / 10 pDONR223-PRKX
Spectinomycin
Plate 1 / C / 11 pDONR223-PANK3
Spectinomycin
Plate 1 / C / 12 pDONR223-PHKG1
Spectinomycin
Plate 1 / D / 1 pDONR223-CIB4
Spectinomycin
Plate 1 / D / 2 pDONR223-PKDCC
Spectinomycin
Plate 1 / D / 3 pDONR223-NUAK2
Spectinomycin
Plate 1 / D / 4 pDONR223-CDKL1
Spectinomycin
Plate 1 / D / 5 pDONR223-DAPK2
Spectinomycin
Plate 1 / D / 6 pDONR223-LOC649288
Spectinomycin
Plate 1 / D / 7 pDONR223-NME2
Spectinomycin
Plate 1 / D / 8 pDONR223-ITGB1BP3
Spectinomycin
Plate 1 / D / 9 pDONR223-LOC652799
Spectinomycin
Plate 1 / D / 10 pDONR223-NEK7
Spectinomycin
Plate 1 / D / 11 pDONR223-SLAMF6
Spectinomycin
Plate 1 / D / 12 pDONR223-TSSK2
Spectinomycin
Plate 1 / E / 1 pDONR223-RAF1
Spectinomycin
Plate 1 / E / 2 pDONR223-BMP2KL
Spectinomycin
Plate 1 / E / 3 pDONR223-CSNK1G3
Spectinomycin
Plate 1 / E / 4 pDONR223-ACVR2B
Spectinomycin
Plate 1 / E / 5 pDONR223-GRK7
Spectinomycin
Plate 1 / E / 6 pDONR223-PAK6
Spectinomycin
Plate 1 / E / 7 pDONR223-MARK2
Spectinomycin
Plate 1 / E / 8 pDONR223-SNRK
Spectinomycin
Plate 1 / E / 9 pDONR223-MAP2K1
Spectinomycin
Plate 1 / E / 10 pDONR223-PLXNB2
Spectinomycin
Plate 1 / E / 11 pDONR223-CAMK2A
Spectinomycin
Plate 1 / E / 12 pDONR223-CAMK2G
Spectinomycin
Plate 1 / F / 1 pDONR223-BRD3
Spectinomycin
Plate 1 / F / 2 pDONR223-MPP7
Spectinomycin
Plate 1 / F / 3 pDONR223-IRAK2
Spectinomycin
Plate 1 / F / 4 pDONR223-PRKG1
Spectinomycin
Plate 1 / F / 5 pDONR223-PFKFB1
Spectinomycin
Plate 1 / F / 6 pDONR223-IPPK
Spectinomycin
Plate 1 / F / 7 pDONR223-MPP6
Spectinomycin
Plate 1 / F / 8 pDONR223-MPP4
Spectinomycin
Plate 1 / F / 9 pDONR223-NEK8
Spectinomycin
Plate 1 / F / 10 pDONR223-PANK4
Spectinomycin
Plate 1 / F / 11 pDONR223-MAPK8
Spectinomycin
Plate 1 / F / 12 pDONR223-CDKL2
Spectinomycin
Plate 1 / G / 1 pDONR223-CAMKK2
Spectinomycin
Plate 1 / G / 2 pDONR223-BMP2K
Spectinomycin
Plate 1 / G / 3 pDONR223-PRKCQ
Spectinomycin
Plate 1 / G / 4 pDONR223-LIMK2
Spectinomycin
Plate 1 / G / 5 pDONR223-MAPKAPK2
Spectinomycin
Plate 1 / G / 6 pDONR223-PGK1
Spectinomycin
Plate 1 / G / 7 pDONR223-CHKA
Spectinomycin
Plate 1 / G / 8 pDONR223-CDC2L6
Spectinomycin
Plate 1 / G / 9 pDONR223-ADRBK1
Spectinomycin
Plate 1 / G / 10 pDONR223-MPP3
Spectinomycin
Plate 1 / G / 11 pDONR223-BRSK2
Spectinomycin
Plate 1 / G / 12 pDONR223-UHMK1
Spectinomycin
Plate 1 / H / 1 pDONR223-NEK5
Spectinomycin
Plate 1 / H / 2 pDONR223-PRKG2
Spectinomycin
Plate 1 / H / 3 pDONR223-DAPK3
Spectinomycin
Plate 1 / H / 4 pDONR223-NEK10
Spectinomycin
Plate 1 / H / 5 pDONR223-TGFBR1
Spectinomycin
Plate 1 / H / 6 pDONR223-PAK3
Spectinomycin
Plate 1 / H / 7 pDONR223-SRPK3
Spectinomycin
Plate 1 / H / 8 pDONR223-MAPK4
Spectinomycin
Plate 1 / H / 9 pDONR223-RIPK1
Spectinomycin
Plate 1 / H / 10 pDONR223-KSR
Spectinomycin
Plate 1 / H / 11 pDONR223-MAPK6
Spectinomycin
Plate 1 / H / 12 pDONR223-DGKA
Spectinomycin
Plate 2 / A / 1 pDONR223-SNF1LK
Spectinomycin
Plate 2 / A / 2 pDONR223-GTF2H1
Spectinomycin
Plate 2 / A / 3 pDONR223-RKHD3
Spectinomycin
Plate 2 / A / 4 pDONR223-MPP5
Spectinomycin
Plate 2 / A / 5 pDONR223-GNE
Spectinomycin
Plate 2 / A / 6 pDONR223-IHPK3
Spectinomycin
Plate 2 / A / 7 pDONR223-PIP5K2C
Spectinomycin
Plate 2 / A / 8 pDONR223-DLG3
Spectinomycin
Plate 2 / A / 9 pDONR223-PRKR
Spectinomycin
Plate 2 / A / 10 pDONR223-AMHR2
Spectinomycin
Plate 2 / A / 11 pDONR223-MAP3K2
Spectinomycin
Plate 2 / A / 12 pDONR223-BRD4
Spectinomycin
Plate 2 / B / 1 pDONR223-MAST2
Spectinomycin
Plate 2 / B / 2 pDONR223-PIK3C3
Spectinomycin
Plate 2 / B / 3 pDONR223-SCYL2
Spectinomycin
Plate 2 / B / 4 pDONR223-NEK9
Spectinomycin
Plate 2 / B / 5 pDONR223-RPS6KC1
Spectinomycin
Plate 2 / B / 6 pDONR223-PIK4CB
Spectinomycin
Plate 2 / B / 7 pDONR223-EIF2AK4
Spectinomycin
Plate 2 / B / 8 pDONR223-CKS2
Spectinomycin
Plate 2 / B / 9 pDONR223-RPS6KL1
Spectinomycin
Plate 2 / B / 10 pDONR223-PRPF4B
Spectinomycin
Plate 2 / B / 11 pDONR223-PIK3CA
Spectinomycin
Plate 2 / B / 12 pDONR223-HIPK3
Spectinomycin
Plate 2 / C / 1 pDONR223-ALDH18A1
Spectinomycin
Plate 2 / C / 2 pDONR223-TNNI3K
Spectinomycin
Plate 2 / C / 3 pDONR223-CASK
Spectinomycin
Plate 2 / C / 4 pDONR223-PHKA1
Spectinomycin
Plate 2 / C / 5 pDONR223-GSG2
Spectinomycin
Plate 2 / C / 6 pDONR223-MAP3K11
Spectinomycin
Plate 2 / C / 7 pDONR223-ULK2
Spectinomycin
Plate 2 / C / 8 pDONR223-DKFZp761P0423
Spectinomycin
Plate 2 / C / 9 pDONR223-TRPM7
Spectinomycin
Plate 2 / C / 10 pDONR223-DGKB
Spectinomycin
Plate 2 / C / 11 pDONR223-TGFBR3
Spectinomycin
Plate 2 / C / 12 pDONR223-NPR2
Spectinomycin
Plate 2 / D / 1 pDONR223-DAPK1
Spectinomycin
Plate 2 / D / 2 pDONR223-HIPK2
Spectinomycin
Plate 2 / D / 3 pDONR223-CARD11
Spectinomycin
Plate 2 / D / 4 pDONR223-PIK3C2G
Spectinomycin
Plate 2 / D / 5 pDONR223-MAP4K1
Spectinomycin
Plate 2 / D / 6 pDONR223-LATS2
Spectinomycin
Plate 2 / D / 7 pDONR223-MAP4K4
Spectinomycin
Plate 2 / D / 8 pDONR223-DGKK
Spectinomycin
Plate 2 / D / 9 pDONR223-PIK3R4
Spectinomycin
Plate 2 / D / 10 pDONR223-LRGUK
Spectinomycin
Plate 2 / D / 11 pDONR223-PRKD2
Spectinomycin
Plate 2 / D / 12 pDONR223-ERN1
Spectinomycin
Plate 2 / E / 1 pDONR223-KIAA2002
Spectinomycin
Plate 2 / E / 2 pDONR223-ALPK2
Spectinomycin
Plate 2 / E / 3 pDONR223-BRAF
Spectinomycin
Plate 2 / E / 4 pDONR223-PRKACA
Spectinomycin
Plate 2 / E / 5 pDONR223-VRK1
Spectinomycin
Plate 2 / E / 6 pDONR223-NEK4
Spectinomycin
Plate 2 / E / 7 pDONR223-MAPK1
Spectinomycin
Plate 2 / E / 8 pDONR223-MAP3K15
Spectinomycin
Plate 2 / E / 9 pDONR223-PRKD1
Spectinomycin
Plate 2 / E / 10 pDONR223-KSR2
Spectinomycin
Plate 2 / E / 11 pDONR223-CHEK2
Spectinomycin
Plate 2 / E / 12 pDONR223-PI4KII
Spectinomycin
Plate 2 / F / 1 pDONR223-PINK1
Spectinomycin
Plate 2 / F / 2 pDONR223-DLG1
Spectinomycin
Plate 2 / F / 3 pDONR223-PRKY
Spectinomycin
Plate 2 / F / 4 pDONR223-PI4K2B
Spectinomycin
Plate 2 / F / 5 pDONR223-DKFZp434B1231
Spectinomycin
Plate 2 / F / 6 pDONR223-MAPK3
Spectinomycin
Plate 2 / F / 7 pDONR223-GSK3A
Spectinomycin
Plate 2 / F / 8 pDONR223-LIMK1
Spectinomycin
Plate 2 / F / 9 pDONR223-AAK1
Spectinomycin
Plate 2 / F / 10 pDONR223-LOC648152
Spectinomycin
Plate 2 / F / 11 pDONR223-PSKH1
Spectinomycin
Plate 2 / F / 12 pDONR223-MASTL
Spectinomycin
Plate 2 / G / 1 pDONR223-PCTK2
Spectinomycin
Plate 2 / G / 2 pDONR223-MAP3K5
Spectinomycin
Plate 2 / G / 3 pDONR223-PLXNA3
Spectinomycin
Plate 2 / G / 4 pDONR223-TPR
Spectinomycin
Plate 2 / G / 5 pDONR223-LRRK2
Spectinomycin
Plate 2 / G / 6 pDONR223-LRPPRC
Spectinomycin
Plate 2 / G / 7 pDONR223-MINK1
Spectinomycin
Plate 2 / G / 8 pDONR223-MAGI1
Spectinomycin
Plate 2 / G / 9 pDONR223-MYO3B
Spectinomycin
Plate 2 / G / 10 pDONR223-CDC42BPG
Spectinomycin
Plate 2 / G / 11 pDONR223-PHKA2
Spectinomycin
Plate 2 / G / 12 pDONR223-RPS6KA3
Spectinomycin
Plate 2 / H / 1 pDONR223-RIPK2
Spectinomycin
Plate 2 / H / 2 pDONR223-DGKG
Spectinomycin
Plate 2 / H / 3 pDONR223-RPS6KA2
Spectinomycin
Plate 2 / H / 4 pDONR223-MKNK1
Spectinomycin
Plate 2 / H / 5 pDONR223-AURKA
Spectinomycin
Plate 2 / H / 6 pDONR223-RPS6KB2
Spectinomycin
Plate 2 / H / 7 pDONR223-VRK2
Spectinomycin
Plate 2 / H / 8 pDONR223-RIOK2
Spectinomycin
Plate 2 / H / 9 pDONR223-IKBKE
Spectinomycin
Plate 2 / H / 10 pDONR223-RIOK3
Spectinomycin
Plate 2 / H / 11 pDONR223-OXSR1
Spectinomycin
Plate 2 / H / 12 pDONR223-PIP5K1B
Spectinomycin
Plate 3 / A / 1 pDONR223-MAP3K8
Spectinomycin
Plate 3 / A / 2 pDONR223-AKT3
Spectinomycin
Plate 3 / A / 3 pDONR223-RPS6KA6
Spectinomycin
Plate 3 / A / 4 pDONR223-PIK4CA
Spectinomycin
Plate 3 / A / 5 pDONR223-PIK3CB
Spectinomycin
Plate 3 / A / 6 pDONR223-PAK1
Spectinomycin
Plate 3 / A / 7 pDONR223-CAMK1
Spectinomycin
Plate 3 / A / 8 pDONR223-LOC340371
Spectinomycin
Plate 3 / A / 9 pDONR223-LOC91807
Spectinomycin
Plate 3 / A / 10 pDONR223-MORN2
Spectinomycin
Plate 3 / A / 11 pDONR223-ULK4
Spectinomycin
Plate 3 / A / 12 pDONR223-PRKAG3
Spectinomycin
Plate 3 / B / 1 pDONR223-PRKCA
Spectinomycin
Plate 3 / B / 2 pDONR223-PRKCE
Spectinomycin
Plate 3 / B / 3 pDONR223-CCL2
Spectinomycin
Plate 3 / B / 4 pDONR223-TSSK3
Spectinomycin
Plate 3 / B / 5 pDONR223-GK5
Spectinomycin
Plate 3 / B / 6 pDONR223-MAP2K2
Spectinomycin
Plate 3 / B / 7 pDONR223-PDK3
Spectinomycin
Plate 3 / B / 8 pDONR223-FLJ10986
Spectinomycin
Plate 3 / B / 9 pDONR223-IHPK1
Spectinomycin
Plate 3 / B / 10 pDONR223-NUP62
Spectinomycin
Plate 3 / B / 11 pDONR223-FASTKD5
Spectinomycin
Plate 3 / B / 12 pDONR223-RP6-213H19.1
Spectinomycin
Plate 3 / C / 1 pDONR223-NME6
Spectinomycin
Plate 3 / C / 2 pDONR223-DTYMK
Spectinomycin
Plate 3 / C / 3 pDONR223-AK3L1
Spectinomycin
Plate 3 / C / 4 pDONR223-STK16
Spectinomycin
Plate 3 / C / 5 pDONR223-SH3BP5L
Spectinomycin
Plate 3 / C / 6 pDONR223-ACVR1B
Spectinomycin
Plate 3 / C / 7 pDONR223-NRBP
Spectinomycin
Plate 3 / C / 8 pDONR223-COL4A3BP
Spectinomycin
Plate 3 / C / 9 pDONR223-PAK7
Spectinomycin
Plate 3 / C / 10 pDONR223-ASCIZ
Spectinomycin
Plate 3 / C / 11 pDONR223-PNCK
Spectinomycin
Plate 3 / C / 12 pDONR223-NME1
Spectinomycin
Plate 3 / D / 1 pDONR223-NME3
Spectinomycin
Plate 3 / D / 2 pDONR223-AK1
Spectinomycin
Plate 3 / D / 3 pDONR223-TBK1
Spectinomycin
Plate 3 / D / 4 pDONR223-PDK4
Spectinomycin
Plate 3 / D / 5 pDONR223-AGK
Spectinomycin
Plate 3 / D / 6 pDONR223-SH3BP5
Spectinomycin
Plate 3 / D / 7 pDONR223-PRKD3
Spectinomycin
Plate 3 / D / 8 pDONR223-PAPSS2
Spectinomycin
Plate 3 / D / 9 pDONR223-SRPK1
Spectinomycin
Plate 3 / D / 10 pDONR223-PANK2
Spectinomycin
Plate 3 / D / 11 pDONR223-NME4
Spectinomycin
Plate 3 / D / 12 pDONR223-DCAKD
Spectinomycin
Plate 3 / E / 1 pDONR223-KHK
Spectinomycin
Plate 3 / E / 2 pDONR223-PIM2
Spectinomycin
Plate 3 / E / 3 pDONR223-PRPS1
Spectinomycin
Plate 3 / E / 4 pDONR223-TRIB2
Spectinomycin
Plate 3 / E / 5 pDONR223-CD2
Spectinomycin
Plate 3 / E / 6 pDONR223-PDGFRL
Spectinomycin
Plate 3 / E / 7 pDONR223-KIAA0999
Spectinomycin
Plate 3 / E / 8 pDONR223-HIPK1
Spectinomycin
Plate 3 / E / 9 pDONR223-MAP3K6
Spectinomycin
Plate 3 / E / 10 pDONR223-STK24
Spectinomycin
Plate 3 / E / 11 pDONR223-MGC42105
Spectinomycin
Plate 3 / E / 12 pDONR223-BRSK1
Spectinomycin
Plate 3 / F / 1 pDONR223-C9orf98
Spectinomycin
Plate 3 / F / 2 pDONR223-SGK3
Spectinomycin
Plate 3 / F / 3 pDONR223-FGFRL1
Spectinomycin
Plate 3 / F / 4 pDONR223-PI4KAP2
Spectinomycin
Plate 3 / F / 5 pDONR223-TXNDC3
Spectinomycin
Plate 3 / F / 6 pDONR223-FASTKD1
Spectinomycin
Plate 3 / F / 7 pDONR223-DCAMKL2
Spectinomycin
Plate 3 / F / 8 pDONR223-FASTKD2
Spectinomycin
Plate 3 / F / 9 pDONR223-HSPB8
Spectinomycin
Plate 3 / F / 10 pDONR223-TRIM27
Spectinomycin
Plate 3 / F / 11 pDONR223-PLXNA4B
Spectinomycin
Plate 3 / F / 12 pDONR223-CABC1
Spectinomycin
Plate 3 / G / 1 pDONR223-MGC16169
Spectinomycin
Plate 3 / G / 2 pDONR223-MAP4K5
Spectinomycin
Plate 3 / G / 3 pDONR223-BUB1
Spectinomycin
Plate 3 / G / 4 pDONR223-HKDC1
Spectinomycin
Plate 3 / G / 5 pDONR223-SCYL1
Spectinomycin
Plate 3 / G / 6 pDONR223-ACVR1C
Spectinomycin
Plate 3 / G / 7 pDONR223-YSK4
Spectinomycin
Plate 3 / G / 8 pDONR223-SNX16
Spectinomycin
Plate 3 / G / 9 pDONR223-BMPR1A
Spectinomycin
Plate 3 / G / 10 pDONR223-ARSG
Spectinomycin
Plate 3 / G / 11 pDONR223-FXN
Spectinomycin
Plate 3 / G / 12 pDONR223-FN3K
Spectinomycin
Plate 3 / H / 1 pDONR223-PRPS2
Spectinomycin
Plate 3 / H / 2 pDONR223-TGFBR2
Spectinomycin
Plate 3 / H / 3 pDONR223-MAK
Spectinomycin
Plate 3 / H / 4 pDONR223-PGK2
Spectinomycin
Plate 3 / H / 5 pDONR223-MAP3K12
Spectinomycin
Plate 3 / H / 6 pDONR223-IRAK3
Spectinomycin
Plate 3 / H / 7 pDONR223-CDKL3
Spectinomycin
Plate 3 / H / 8 pDONR223-TLK2
Spectinomycin
Plate 3 / H / 9 pDONR223-ALPK1
Spectinomycin
Plate 3 / H / 10 pDONR223-PIP5KL1
Spectinomycin
Plate 3 / H / 11 pDONR223-HK2
Spectinomycin
Plate 3 / H / 12 pDONR223-MAST1
Spectinomycin
Plate 4 / A / 1 pDONR223-ALS2CR7
Spectinomycin
Plate 4 / A / 2 pDONR223-LYK5
Spectinomycin
Plate 4 / A / 3 pDONR223-ETNK1
Spectinomycin
Plate 4 / A / 4 pDONR223-DYRK3
Spectinomycin
Plate 4 / A / 5 pDONR223-RP2
Spectinomycin
Plate 4 / A / 6 pDONR223-MAPKAPK5
Spectinomycin
Plate 4 / A / 7 pDONR223-BMPR1B
Spectinomycin
Plate 4 / A / 8 pDONR223-XYLB
Spectinomycin
Plate 4 / A / 9 pDONR223-PRKCG
Spectinomycin
Plate 4 / A / 10 pDONR223-RPS6KA4
Spectinomycin
Plate 4 / A / 11 pDONR223-NLK
Spectinomycin
Plate 4 / A / 12 pDONR223-SH3BP4
Spectinomycin
Plate 4 / B / 1 pDONR223-MAP4K2
Spectinomycin
Plate 4 / B / 2 pDONR223-RAGE
Spectinomycin
Plate 4 / B / 3 pDONR223-ULK3
Spectinomycin
Plate 4 / B / 4 pDONR223-PRKAB2
Spectinomycin
Plate 4 / B / 5 pDONR223-NJMU-R1
Spectinomycin
Plate 4 / B / 6 pDONR223-MAPK10
Spectinomycin
Plate 4 / B / 7 pDONR223-ADCK1
Spectinomycin
Plate 4 / B / 8 pDONR223-PTK9
Spectinomycin
Plate 4 / B / 9 pDONR223-CDK10
Spectinomycin
Plate 4 / B / 10 pDONR223-GLYCTK
Spectinomycin
Plate 4 / B / 11 pDONR223-ACVR2A
Spectinomycin
Plate 4 / B / 12 pDONR223-PAK2
Spectinomycin
Plate 4 / C / 1 pDONR223-MYLK2
Spectinomycin
Plate 4 / C / 2 pDONR223-MOS
Spectinomycin
Plate 4 / C / 3 pDONR223-NEK2
Spectinomycin
Plate 4 / C / 4 pDONR223-TPK1
Spectinomycin
Plate 4 / C / 5 pDONR223-COASY
Spectinomycin
Plate 4 / C / 6 pDONR223-PIK3CG
Spectinomycin
Plate 4 / C / 7 pDONR223-RIPK5
Spectinomycin
Plate 4 / C / 8 pDONR223-TTK
Spectinomycin
Plate 4 / C / 9 pDONR223-MAP4K3
Spectinomycin
Plate 4 / C / 10 pDONR223-LATS1
Spectinomycin
Plate 4 / C / 11 pDONR223-IPMK
Spectinomycin
Plate 4 / C / 12 pDONR223-PRKAR2B
Spectinomycin
Plate 4 / D / 1 pDONR223-PFKFB3
Spectinomycin
Plate 4 / D / 2 pDONR223-BMPR2
Spectinomycin
Plate 4 / D / 3 pDONR223-WNK1
Spectinomycin
Plate 4 / D / 4 pDONR223-PRKAA2
Spectinomycin
Plate 4 / D / 5 pDONR223-PKLR
Spectinomycin
Plate 4 / D / 6 pDONR223-MPP2
Spectinomycin
Plate 4 / D / 7 pDONR223-EXOSC10
Spectinomycin
Plate 4 / D / 8 pDONR223-XRCC6BP1
Spectinomycin
Plate 4 / D / 9 pDONR223-AK2
Spectinomycin
Plate 4 / D / 10 pDONR223-TRIB3
Spectinomycin
Plate 4 / D / 11 pDONR223-CSNK2A1
Spectinomycin
Plate 4 / D / 12 pDONR223-FN3KRP
Spectinomycin
Plate 4 / E / 1 pDONR223-SCYL3
Spectinomycin
Plate 4 / E / 2 pDONR223-RBKS
Spectinomycin
Plate 4 / E / 3 pDONR223-AURKB
Spectinomycin
Plate 4 / E / 4 pDONR223-BUB1B
Spectinomycin
Plate 4 / E / 5 pDONR223-FUK
Spectinomycin
Plate 4 / E / 6 pDONR223-CLK2
Spectinomycin
Plate 4 / E / 7 pDONR223-RPS6KB1
Spectinomycin
Plate 4 / E / 8 pDONR223-VRK3
Spectinomycin
Plate 4 / E / 9 pDONR223-CDK6
Spectinomycin
Plate 4 / E / 10 pDONR223-PRKAG2
Spectinomycin
Plate 4 / E / 11 pDONR223-DYRK2
Spectinomycin
Plate 4 / E / 12 pDONR223-PLK2
Spectinomycin
Plate 4 / F / 1 pDONR223-PIM1
Spectinomycin
Plate 4 / F / 2 pDONR223-MAP3K7
Spectinomycin
Plate 4 / F / 3 pDONR223-MAP2K1IP1
Spectinomycin
Plate 4 / F / 4 pDONR223-TESK2
Spectinomycin
Plate 4 / F / 5 pDONR223-PIK3R1
Spectinomycin
Plate 4 / F / 6 pDONR223-MAPK15
Spectinomycin
Plate 4 / F / 7 pDONR223-RFK
Spectinomycin
Plate 4 / F / 8 pDONR223-CDK5
Spectinomycin
Plate 4 / F / 9 pDONR223-TWF2
Spectinomycin
Plate 4 / F / 10 pDONR223-CDK9
Spectinomycin
Plate 4 / F / 11 pDONR223-NME7
Spectinomycin
Plate 4 / F / 12 pDONR223-MVK
Spectinomycin
Plate 4 / G / 1 pDONR223-SPHK1
Spectinomycin
Plate 4 / G / 2 pDONR223-CSNK1G2
Spectinomycin
Plate 4 / G / 3 pDONR223-CKMT1A
Spectinomycin
Plate 4 / G / 4 pDONR223-PHKB
Spectinomycin
Plate 4 / G / 5 pDONR223-SGK
Spectinomycin
Plate 4 / G / 6 pDONR223-ZAK
Spectinomycin
Plate 4 / G / 7 pDONR223-PCTK3
Spectinomycin
Plate 4 / G / 8 pDONR223-ADCK4
Spectinomycin
Plate 4 / G / 9 pDONR223-FASTK
Spectinomycin
Plate 4 / G / 10 pDONR223-PAK4
Spectinomycin
Plate 4 / G / 11 pDONR223-SPHK2
Spectinomycin
Plate 4 / G / 12 pDONR223-PCK2
Spectinomycin
Plate 4 / H / 1 pDONR223-MARK3
Spectinomycin
Plate 4 / H / 2 pDONR223-CCRK
Spectinomycin
Plate 4 / H / 3 pDONR223-PRKAG1
Spectinomycin
Plate 4 / H / 4 pDONR223-MAPK12
Spectinomycin
Plate 4 / H / 5 pDONR223-STK32C
Spectinomycin
Plate 4 / H / 6 pDONR223-STK17B
Spectinomycin
Plate 4 / H / 7 pDONR223-ADCK2
Spectinomycin
Plate 4 / H / 8 pDONR223-PXK
Spectinomycin
Plate 4 / H / 9 pDONR223-PRKCZ
Spectinomycin
Plate 4 / H / 10 pDONR223-ARAF
Spectinomycin
Plate 4 / H / 11 pDONR223-RPS6KA1
Spectinomycin
Plate 4 / H / 12 pDONR223-TAOK3
Spectinomycin
Plate 5 / A / 1 pDONR223-EEF2K
Spectinomycin
Plate 5 / A / 2 pDONR223-TLK1
Spectinomycin
Plate 5 / A / 3 pDONR223-PFKM
Spectinomycin
Plate 5 / A / 4 pDONR223-PFKL
Spectinomycin
Plate 5 / A / 5 pDONR223-HK1
Spectinomycin
Plate 5 / A / 6 pDONR223-HK3
Spectinomycin
Plate 5 / A / 7 pDONR223-MAP3K14
Spectinomycin
Plate 5 / A / 8 pDONR223-NYD-SP25
Spectinomycin
Plate 5 / A / 9 pDONR223-CALM2
Spectinomycin
Plate 5 / A / 10 pDONR223-MKNK2
Spectinomycin
Plate 5 / A / 11 pDONR223-TK1
Spectinomycin
Plate 5 / A / 12 pDONR223-CSNK1G1
Spectinomycin
Plate 5 / B / 1 pDONR223-PDIK1L
Spectinomycin
Plate 5 / B / 2 pDONR223-MAPK13
Spectinomycin
Plate 5 / B / 3 pDONR223-TSSK1
Spectinomycin
Plate 5 / B / 4 pDONR223-PRKAR1A
Spectinomycin
Plate 5 / B / 5 pDONR223-CKB
Spectinomycin
Plate 5 / B / 6 pDONR223-GALK1
Spectinomycin
Plate 5 / B / 7 pDONR223-PIP5K2A
Spectinomycin
Plate 5 / B / 8 pDONR223-PHKG2
Spectinomycin
Plate 5 / B / 9 pDONR223-CKMT2
Spectinomycin
Plate 5 / B / 10 pDONR223-NADK
Spectinomycin
Plate 5 / B / 11 pDONR223-GALK2
Spectinomycin
Plate 5 / B / 12 pDONR223-IRAK4
Spectinomycin
Plate 5 / C / 1 pDONR223-GCK
Spectinomycin
Plate 5 / C / 2 pDONR223-MPP1
Spectinomycin
Plate 5 / C / 3 pDONR223-AKT1
Spectinomycin
Plate 5 / C / 4 pDONR223-CLK3
Spectinomycin
Plate 5 / C / 5 pDONR223-PCTK1
Spectinomycin
Plate 5 / C / 6 pDONR223-PIP5K1A
Spectinomycin
Plate 5 / C / 7 pDONR223-CAMKV
Spectinomycin
Plate 5 / C / 8 pDONR223-PKM2
Spectinomycin
Plate 5 / C / 9 pDONR223-RPS6KA5
Spectinomycin
Plate 5 / C / 10 pDONR223-RIOK1
Spectinomycin
Plate 5 / C / 11 pDONR223-HIPK4
Spectinomycin
Plate 5 / C / 12 pDONR223-DYRK1B
Spectinomycin
Plate 5 / D / 1 pDONR223-ITPKB
Spectinomycin
Plate 5 / D / 2 pDONR223-AK7
Spectinomycin
Plate 5 / D / 3 pDONR223-PRKCB2
Spectinomycin
Plate 5 / D / 4 pDONR223-PRKCH
Spectinomycin
Plate 5 / D / 5 pDONR223-SRPK2
Spectinomycin
Plate 5 / D / 6 pDONR223-CKS1B
Spectinomycin
Plate 5 / D / 7 pDONR223-CALM1
Spectinomycin
Plate 5 / D / 8 pDONR223-CALM3
Spectinomycin
Plate 5 / D / 9 pDONR223-STK32A
Spectinomycin
Plate 5 / D / 10 pDONR223-PMVK
Spectinomycin
Plate 5 / D / 11 pDONR223-NME5
Spectinomycin
Plate 5 / D / 12 pDONR223-AK3
Spectinomycin
Plate 5 / E / 1 pDONR223-TP53RK
Spectinomycin
Plate 5 / E / 2 pDONR223-TSSK6
Spectinomycin
Plate 5 / E / 3 pDONR223-CDC2
Spectinomycin
Plate 5 / E / 4 pDONR223-CDK2
Spectinomycin
Plate 5 / E / 5 pDONR223-CDK4
Spectinomycin
Plate 5 / E / 6 pDONR223-CDK5R1
Spectinomycin
Plate 5 / E / 7 pDONR223-PDXK
Spectinomycin
Plate 5 / E / 8 pDONR223-NEK6
Spectinomycin
Plate 5 / E / 9 pDONR223-PBK
Spectinomycin
Plate 5 / E / 10 pDONR223-MAP2K6
Spectinomycin
Plate 5 / E / 11 pDONR223-CSNK1A1L
Spectinomycin
Plate 5 / E / 12 pDONR223-NAGK
Spectinomycin
Plate 5 / F / 1 pDONR223-CDK7
Spectinomycin
Plate 5 / F / 2 pDONR223-PRKACB
Spectinomycin
Plate 5 / F / 3 pDONR223-PRKACG
Spectinomycin
Plate 5 / F / 4 pDONR223-PRKAR2A
Spectinomycin
Plate 5 / F / 5 pDONR223-MAPKAPK3
Spectinomycin
Plate 5 / F / 6 pDONR223-CAMK1D
Spectinomycin
Plate 5 / F / 7 pDONR223-ETNK2
Spectinomycin
Plate 5 / F / 8 pDONR223-ADRBK2
Spectinomycin
Plate 5 / F / 9 pDONR223-BCKDK
Spectinomycin
Plate 5 / F / 10 pDONR223-STK32B
Spectinomycin
Plate 5 / F / 11 pDONR223-CSNK1D
Spectinomycin
Plate 5 / F / 12 pDONR223-CSNK1E
Spectinomycin
Plate 5 / G / 1 pDONR223-MAP2K7
Spectinomycin
Plate 5 / G / 2 pDONR223-IHPK2
Spectinomycin
Plate 5 / G / 3 pDONR223-STK25
Spectinomycin
Plate 5 / G / 4 pDONR223-PDPK1
Spectinomycin
Plate 5 / G / 5 pDONR223-STK11
Spectinomycin
Plate 5 / G / 6 pDONR223-STK40
Spectinomycin
Plate 5 / G / 7 pDONR223-PDK1
Spectinomycin
Plate 5 / G / 8 pDONR223-MAP2K5
Spectinomycin
Plate 5 / G / 9 pDONR223-PIP5K3
Spectinomycin
Plate 5 / G / 10 pDONR223-ILK
Spectinomycin
Plate 5 / G / 11 pDONR223-PIK3R3
Spectinomycin
Plate 5 / G / 12 pDONR223-ADPGK
Spectinomycin
Plate 5 / H / 1 pDONR223-STK38L
Spectinomycin
Plate 5 / H / 2 pDONR223-STK38
Spectinomycin
Plate 5 / H / 3 pDONR223-CAMK4
Spectinomycin
Plate 5 / H / 4 pDONR223-CAMK1G
Spectinomycin
Plate 5 / H / 5 pDONR223-CAMK2D
Spectinomycin
Plate 5 / H / 6 pDONR223-UCK2
Spectinomycin
Plate 5 / H / 7 pDONR223-NEK11
Spectinomycin
Plate 5 / H / 8 pDONR223-CLK1
Spectinomycin
Plate 5 / H / 9 pDONR223-STK3
Spectinomycin
Plate 5 / H / 10 pDONR223-PIK3R5
Spectinomycin
Plate 5 / H / 11 pDONR223-DGUOK
Spectinomycin
Plate 5 / H / 12 pDONR223-MAPK14
Spectinomycin
Plate 6 / A / 1 pDONR223-ERBB3
Spectinomycin
Plate 6 / A / 2 pDONR223-ERBB4
Spectinomycin
Plate 6 / A / 3 pDONR223-FES
Spectinomycin
Plate 6 / A / 4 pDONR223-FGR
Spectinomycin
Plate 6 / A / 5 pDONR223-FRK
Spectinomycin
Plate 6 / A / 6 pDONR223-HCK
Spectinomycin
Plate 6 / A / 7 pDONR223-CKM
Spectinomycin
Plate 6 / A / 8 pDONR223-ITK
Spectinomycin
Plate 6 / A / 9 pDONR223-MATK
Spectinomycin
Plate 6 / A / 10 pDONR223-NTRK2
Spectinomycin
Plate 6 / A / 11 pDONR223-PKMYT1
Spectinomycin
Plate 6 / A / 12 pDONR223-TEC
Spectinomycin
Plate 6 / B / 1 pDONR223-TXK
Spectinomycin
Plate 6 / B / 2 pDONR223-ZAP70
Spectinomycin
Plate 6 / B / 3 pDONR223-ERBB2
Spectinomycin
Plate 6 / B / 4 pDONR223-MET
Spectinomycin
Plate 6 / B / 5 pDONR223-LCK
Spectinomycin
Plate 6 / B / 6 pDONR223-NTRK1
Spectinomycin
Plate 6 / B / 7 pDONR223-PDGFRA
Spectinomycin
Plate 6 / B / 8 pDONR223-PDGFRB
Spectinomycin
Plate 6 / B / 9 pDONR223-TNK1
Spectinomycin
Plate 6 / B / 10 pDONR223-FLT3
Spectinomycin
Plate 6 / B / 11 pDONR223-EPHB4
Spectinomycin
Plate 6 / B / 12 pDONR223-DDR2
Spectinomycin
Plate 6 / C / 1 pDONR223-PTK2B
Spectinomycin
Plate 6 / C / 2 pDONR223-PTK6
Spectinomycin
Plate 6 / C / 3 pDONR223-MERTK
Spectinomycin
Plate 6 / C / 4 pDONR223-NTRK3
Spectinomycin
Plate 6 / C / 5 pDONR223-PTK2
Spectinomycin
Plate 6 / C / 6 pDONR223-STYK1
Spectinomycin
Plate 6 / C / 7 pDONR223-FGFR2
Spectinomycin
Plate 6 / C / 8 pDONR223-LYN
Spectinomycin
Plate 6 / C / 9 pDONR223-RET
Spectinomycin
Plate 6 / C / 10 pDONR223-SYK
Spectinomycin
Plate 6 / C / 11 pDONR223-TYK2
Spectinomycin
Plate 6 / C / 12 pDONR223-ABL2
Spectinomycin
Plate 6 / D / 1 pDONR223-DDR1
Spectinomycin
Plate 6 / D / 2 pDONR223-EPHA3
Spectinomycin
Plate 6 / D / 3 pDONR223-FLT1
Spectinomycin
Plate 6 / D / 4 pDONR223-PLK1
Spectinomycin
Plate 6 / D / 5 pDONR223-LMTK2
Spectinomycin
Plate 6 / D / 6 pDONR223-JAK2
Spectinomycin
Plate 6 / D / 7 pDONR223-TYRO3
Spectinomycin
Plate 6 / D / 8 pDONR223-ALK
Spectinomycin
Plate 6 / D / 9 pDONR223-BTK
Spectinomycin
Plate 6 / D / 10 pDONR223-EPHA4
Spectinomycin
Plate 6 / D / 11 pDONR223-EPHA6
Spectinomycin
Plate 6 / D / 12 pDONR223-FER
Spectinomycin
Plate 6 / E / 1 pDONR223-FGFR1
Spectinomycin
Plate 6 / E / 2 pDONR223-FLT4
Spectinomycin
Plate 6 / E / 3 pDONR223-INSRR
Spectinomycin
Plate 6 / E / 4 pDONR223-KDR
Spectinomycin
Plate 6 / E / 5 pDONR223-EPHA2
Spectinomycin
Plate 6 / E / 6 pDONR223-ROR2
Spectinomycin
Plate 6 / E / 7 pDONR223-CSF1R
Spectinomycin
Plate 6 / E / 8 pDONR223-EPHA1
Spectinomycin
Plate 6 / E / 9 pDONR223-EPHB1
Spectinomycin
Plate 6 / E / 10 pDONR223-EPHB6
Spectinomycin
Plate 6 / E / 11 pDONR223-JAK1
Spectinomycin
Plate 6 / E / 12 pDONR223-FGFR3
Spectinomycin
Plate 6 / F / 1 pDONR223-SRC
Spectinomycin
Plate 6 / F / 2 pDONR223-EGFR
Spectinomycin
Plate 6 / F / 3 pDONR223-BMX
Spectinomycin
Plate 6 / F / 5 pDONR223-YES1
Spectinomycin
Plate 6 / F / 6 pDONR223-ABL1
Spectinomycin
Plate 6 / F / 7 pDONR223-BLK
Spectinomycin
Plate 6 / F / 8 pDONR223-CSK
Spectinomycin
Plate 6 / F / 9 pDONR223-MST1R
Spectinomycin
Plate 6 / F / 10 pDONR223-PTK7
Spectinomycin
Plate 6 / F / 11 pDONR223-JAK3
Spectinomycin
Plate 6 / F / 12 pDONR223-AXL
Spectinomycin
Plate 6 / G / 1 pDONR223-TIE1
Spectinomycin
Plate 6 / G / 2 pDONR223-PAPSS1
Spectinomycin
Plate 6 / G / 3 pDONR223-PFKP
Spectinomycin
Plate 6 / G / 4 pDONR223-DAK
Spectinomycin
Plate 6 / G / 5 pDONR223-PRKAA1
Spectinomycin
Plate 6 / G / 6 pDONR223-GK
Spectinomycin
Plate 6 / G / 7 pDONR223-ACVRL1
Spectinomycin
Data calculated @ 2016-12-06

Kit Plate Map - #1000000014

Control and Expression Vectors

A set of 5 control ORFs ( eGFP , BFP , HcRed , LacZ , Luciferase ) are available to be used as negative controls for experiments with this set. These plasmids are not included in the kit and must be ordered separately.

A set of 4 custom lentiviral vectors is available ( pLX301 , pLX302 , pLX303 , pLX304 ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

There are 3 activated oncogene ORF vectors that can be used with this set as well: K-RAS V12 , H-RAS V12 , MEKDD .

Related links: To learn more about the 15,000+ ORFs contained in the entry clone collection, as well as the entire lentiviral expression collection (15,000+ ORFs) described in the Nature Method paper, please visit the ORFeome Collaboration Website . Plasmids from the full ORF collections can be obtained from ODF/HCC , DNASU , and ThermoFisher .

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