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Yeast GoldenBraid Cloning System and Toolkit
(Kit # 1000000138 )

Depositing Lab:   Elena Caro Bernat

The Yeast GoldenBraid Cloning System and Toolkit provides modular pieces (promoters, MTSs, 8xHis N terminal tag, terminators and resistance cassettes) for the generation of transcriptional units using the GoldenBraid cloning system. This toolkit allows for integration in the S. cerevisiae genome at two different loci supporting high and stable transgene expression: YPRCΔ15 and YORWΔ22.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$330 USD + shipping

Available to academics and nonprofits only.

Original Publication

Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria Pérez-González A., Kniewel R., Veldhuizen M., Verma H.K., Navarro-Rodríguez M., Rubio L.M., & Caro E. BMC Biotechnology. (2017).17(1), 80; doi: 10.1186/s12896-017-0393-y. PubMed PMID: 29132331.

Link to original publication

Description

The Yeast GoldenBraid Cloning System and Toolkit contains:

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Yeast GoldenBraid Cloning System and Toolkit was a gift from Elena Caro Bernat (Addgene kit #1000000138).”

For your Reference section:

Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria Pérez-González A., Kniewel R., Veldhuizen M., Verma H.K., Navarro-Rodríguez M., Rubio L.M., & Caro E. BMC Biotechnology. (2017).17(1), 80; doi: 10.1186/s12896-017-0393-y. PubMed PMID: 29132331.

Yeast GoldenBraid Cloning System and Toolkit - #1000000138

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Chloramphenicol
Spectinomycin
Kanamycin

Inventory

Well Plasmid Resistance
A / 1 pUPD-pGAL1 (01-11)
Ampicillin
A / 2 pUPD-pTDH3 (01-11)
Ampicillin
A / 3 pUPD-pTDH2 (01-11)
Ampicillin
A / 4 pUPD-pTEF2 (01-11)
Ampicillin
A / 5 pUPD-pHXT7 (01-11)
Ampicillin
A / 6 pUPD-pTPI1 (01-11)
Ampicillin
A / 7 pUPD-pPYK1 (01-11)
Ampicillin
A / 8 pUPD-PGK1 (01-11)
Ampicillin
A / 9 pUPD-PGI1 (01-11)
Ampicillin
A / 10 pUPD-Su9MTS (12)
Ampicillin
A / 11 pUPD-SOD2MTS (12)
Ampicillin
A / 12 pUPD-MAM33MTS (12)
Ampicillin
B / 1 pUPD2-ODPAMTS (12)
Chloramphenicol
B / 2 pUPD2-ODPBMTS (12)
Chloramphenicol
B / 3 pUPD2-ATPAMTS (12)
Chloramphenicol
B / 4 pUPD2-GLRX2MTS (12)
Chloramphenicol
B / 5 pUPD-MTS2 (12)
Ampicillin
B / 6 pUC57-8xHis
Ampicillin
B / 7 pUPD-Cyc1t (17-21)
Ampicillin
B / 8 pUPD-ADH2t (17-21)
Ampicillin
B / 9 pUPD-TDH2t (17-21)
Ampicillin
B / 10 pUPD-G418R (01-21)
Ampicillin
B / 11 pUPD-HygroBR (01-21)
Ampicillin
B / 12 YORWΔ22Ω2
Spectinomycin
C / 1 YORWΔ22Ω1
Spectinomycin
C / 2 YORWΔ22α2
Kanamycin
C / 3 YORWΔ22α1
Kanamycin
C / 4 YPRCΔ15Ω2
Spectinomycin
C / 5 YPRCΔ15Ω1
Spectinomycin
C / 6 YPRCΔ15α2
Kanamycin
C / 7 YPRCΔ15α1
Kanamycin
Data calculated @ 2018-12-10

Kit Plate Map - #1000000138

Plate #1

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A
B
C
D
E
F
G
H
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