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The 56 plasmid GreenGate cloning kit can be used to create plant expression vectors containing several cassettes and generate multi-construct transgenic plants.
This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed PMID 24376629
GreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs . It is based on the Golden Gate method. Using the type IIS restriction endonuclease BsaI and T4 DNA ligase, ready-to-use plant transformation vectors are built from six types of pre-cloned insert modules and a destination vector in a one-pot reaction. The six module types are (1) plant promoter, (2) N-terminal tag, (3) coding sequence (i.e. the gene of interest), (4) C-terminal tag, (5) plant terminator and (6) plant resistance cassette.
More information on these plasmids and their use can be found in the original publication.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The plasmid kit used for generation of plant transformation constructs was a gift from Jan Lohmann (Addgene kit # 1000000036)"
For your Reference section: GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J. PLoS One 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed PMID 24376629
GreenGate - #1000000036
Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
Searchable and sortable table of all plasmids in kit. The Well column
lists the plasmid well location in its plate.
The Plasmid column links to a plasmid's individual web page.
Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.