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Marx Lab FREQ-Seq Kit: High Resolution Allele Frequency Measurements
(Kit # 1000000032 )

Depositing Labs:   Christopher Marx

The FREQ-Seq collection can be used to construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing. By counting DNA sequence reads, this strategy allows for direct quantitative allele detection.

This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$165 USD + shipping

Available to academics and nonprofits only.

Original Publication

FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012; 7(10): e47959. PubMed . Open Access Article .

Description

The FREQ-Seq collection can be used to construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing. By counting DNA sequence reads, this strategy allows for direct quantitative allele detection. The kit consists of a 48 plasmid adaptor library, with each plasmid carrying a unique barcoded Illumina-M13F bridging primer. These bridging primers can be amplified and used to generate Illumina sequencing libraries by means of a 2-step PCR-based protocol and are compatible with single-end or paired-end read flow cells.

FreqSeq Figure

Kit Documentation

Icon FREQ-Seq kit.pdf (1.3 MB)

Protocols

An abbreviated FREQ-Seq Protocol from the publication:

Icon marx-lab-freq-seq-protocol.pdf (316.9 KB)

Links

Analysis software: FREQ-Seq Website

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The FREQ-seq plasmids were a gift from Christopher Marx (Addgene kit # 1000000032)"

For your Reference section:
FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012; 7(10): e47959. PubMed . Open Access Article .

FREQ-Seq Kit - #1000000032

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pFSBC1
Ampicillin
A / 2 pFSBC2
Ampicillin
A / 3 pFSBC3
Ampicillin
A / 4 pFSBC4
Ampicillin
A / 5 pFSBC5
Ampicillin
A / 6 pFSBC6
Ampicillin
A / 7 pFSBC7
Ampicillin
A / 8 pFSBC8
Ampicillin
A / 9 pFSBC9
Ampicillin
A / 10 pFSBC10
Ampicillin
A / 11 pFSBC11
Ampicillin
A / 12 pFSBC12
Ampicillin
B / 1 pFSBC13
Ampicillin
B / 2 pFSBC14
Ampicillin
B / 3 pFSBC15
Ampicillin
B / 4 pFSBC16
Ampicillin
B / 5 pFSBC17
Ampicillin
B / 6 pFSBC18
Ampicillin
B / 7 pFSBC19
Ampicillin
B / 8 pFSBC20
Ampicillin
B / 9 pFSBC21
Ampicillin
B / 10 pFSBC22
Ampicillin
B / 11 pFSBC23
Ampicillin
B / 12 pFSBC24
Ampicillin
C / 1 pFSBC25
Ampicillin
C / 2 pFSBC26
Ampicillin
C / 3 pFSBC27
Ampicillin
C / 4 pFSBC28
Ampicillin
C / 5 pFSBC29
Ampicillin
C / 6 pFSBC30
Ampicillin
C / 7 pFSBC31
Ampicillin
C / 8 pFSBC32
Ampicillin
C / 9 pFSBC33
Ampicillin
C / 10 pFSBC34
Ampicillin
C / 11 pFSBC35
Ampicillin
C / 12 pFSBC36
Ampicillin
D / 1 pFSBC37
Ampicillin
D / 2 pFSBC38
Ampicillin
D / 3 pFSBC39
Ampicillin
D / 4 pFSBC40
Ampicillin
D / 5 pFSBC41
Ampicillin
D / 6 pFSBC42
Ampicillin
D / 7 pFSBC43
Ampicillin
D / 8 pFSBC44
Ampicillin
D / 9 pFSBC45
Ampicillin
D / 10 pFSBC46
Ampicillin
D / 11 pFSBC47
Ampicillin
D / 12 pFSBC48
Ampicillin
Data calculated @ 2016-12-06

Kit Plate Map - #1000000032

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