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CIDAR MoClo Extension, Volume I
(Kit # 1000000161 )

Depositing Lab:   Richard Murray

This kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$700 USD + shipping

Available to academics and nonprofits only.

Original Publication

The Richard Murray Lab has released these reagents to the scientific community as an unpublished kit. Please cite "Clamons S. et al., unpublished reagents" if you use these reagents in a subsequent publication. See the CIDAR MoClo Extension, Volume I Guide, linked from the Protocols & Resources tab, for additional information regarding the experimental use of the plasmids included in this kit.

Description

The MoClo system is a specification for plasmid parts that can be easily combined together to form new plasmids with functional expression units (and multi-gene expression systems) using Golden Gate assembly with BsaI and BbsI. While MoClo is used across a variety of systems and organisms, this kit contains parts designed for bacterial expression.

This kit contains 110 plasmids to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system. This kit can be used as a stand-alone product, but is intended to be used with the CIDAR MoClo Parts Kit (Kit #1000000059) from the Douglas Densmore Lab, which contains a number of useful parts not included in this kit.

CIDAR MoClo Extension, Volume I extends the CIDAR kit by adding 21 promoters, 13 UTRs, 60 CDSs, 10 terminators, several combined UTR-CDS parts, and two new destination vectors for easier screening. These parts are backward and forward compatible with parts from the original CIDAR kit.

CIDAR MoClo Extension, Volume I

Kit Documentation

Icon CIDAR MoClo Extension, Volume I Guide (2.4 MB)

Please note that P9m (A4) and P35m (B1) should be grown at 30°C, due to a strong promoter. See the individual plasmid pages for additional information.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the guide in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“CIDAR MoClo Extension, Volume I was a gift from Richard Murray (Addgene kit #1000000161).”

CIDAR MoClo Extension, Volume I - #1000000161

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Kanamycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 P1m
Ampicillin
Plate 1 / A / 2 p3m
Ampicillin
Plate 1 / A / 3 p4m
Ampicillin
Plate 1 / A / 4 P9m
Ampicillin
Plate 1 / A / 5 P12m
Ampicillin
Plate 1 / A / 6 P15m
Ampicillin
Plate 1 / A / 7 P16m
Ampicillin
Plate 1 / A / 8 P18m
Ampicillin
Plate 1 / A / 9 P30m
Ampicillin
Plate 1 / A / 10 P32m
Ampicillin
Plate 1 / A / 11 P33m
Ampicillin
Plate 1 / A / 12 P34m
Ampicillin
Plate 1 / B / 1 P35m
Ampicillin
Plate 1 / B / 2 P37m
Ampicillin
Plate 1 / B / 3 P38m
Ampicillin
Plate 1 / B / 4 P57m
Ampicillin
Plate 1 / B / 5 P58m
Ampicillin
Plate 1 / B / 6 P59m
Ampicillin
Plate 1 / B / 7 P60m
Ampicillin
Plate 1 / B / 8 P61m
Ampicillin
Plate 1 / B / 9 P66m
Ampicillin
Plate 1 / B / 10 C1m
Ampicillin
Plate 1 / B / 11 C3m
Ampicillin
Plate 1 / B / 12 C5m
Ampicillin
Plate 1 / C / 1 C9m
Ampicillin
Plate 1 / C / 2 C11m
Ampicillin
Plate 1 / C / 3 C13m
Ampicillin
Plate 1 / C / 4 C20m
Ampicillin
Plate 1 / C / 5 C21m
Ampicillin
Plate 1 / C / 6 C22m
Ampicillin
Plate 1 / C / 7 C24m
Ampicillin
Plate 1 / C / 8 C27m
Ampicillin
Plate 1 / C / 9 C31m
Ampicillin
Plate 1 / C / 10 C32m
Ampicillin
Plate 1 / C / 11 C33m
Ampicillin
Plate 1 / C / 12 C35m
Ampicillin
Plate 1 / D / 1 C36m
Ampicillin
Plate 1 / D / 2 C38m
Ampicillin
Plate 1 / D / 3 C39m
Ampicillin
Plate 1 / D / 4 C40m
Ampicillin
Plate 1 / D / 5 C41m
Ampicillin
Plate 1 / D / 6 C51m
Ampicillin
Plate 1 / D / 7 C59m
Ampicillin
Plate 1 / D / 8 C61m
Ampicillin
Plate 1 / D / 9 C62m
Ampicillin
Plate 1 / D / 10 C63m
Ampicillin
Plate 1 / D / 11 C64m
Ampicillin
Plate 1 / D / 12 C67m
Ampicillin
Plate 1 / E / 1 C70m
Ampicillin
Plate 1 / E / 2 C71m
Ampicillin
Plate 1 / E / 3 C72m
Ampicillin
Plate 1 / E / 4 C73m
Ampicillin
Plate 1 / E / 5 C74m
Ampicillin
Plate 1 / E / 6 C75m
Ampicillin
Plate 1 / E / 7 C76m
Ampicillin
Plate 1 / E / 8 C77m
Ampicillin
Plate 1 / E / 9 C78m
Ampicillin
Plate 1 / E / 10 C79m
Ampicillin
Plate 1 / E / 11 C80m
Ampicillin
Plate 1 / E / 12 C82m
Ampicillin
Plate 1 / F / 1 C83m
Ampicillin
Plate 1 / F / 2 C84m
Ampicillin
Plate 1 / F / 3 C85m
Ampicillin
Plate 1 / F / 4 C86m
Ampicillin
Plate 1 / F / 5 C90m
Ampicillin
Plate 1 / F / 6 C91m
Ampicillin
Plate 1 / F / 7 C92m
Ampicillin
Plate 1 / F / 8 C95m
Ampicillin
Plate 1 / F / 9 C98m
Ampicillin
Plate 1 / F / 10 C99m
Ampicillin
Plate 1 / F / 11 C100m
Ampicillin
Plate 1 / F / 12 C101m
Ampicillin
Plate 1 / G / 1 C102m
Ampicillin
Plate 1 / G / 2 C103m
Ampicillin
Plate 1 / G / 3 C105m
Ampicillin
Plate 1 / G / 4 C106m
Ampicillin
Plate 1 / G / 5 C108m
Ampicillin
Plate 1 / G / 6 C109m
Ampicillin
Plate 1 / G / 7 C113m
Ampicillin
Plate 1 / G / 8 C114m
Ampicillin
Plate 1 / G / 9 C115m
Ampicillin
Plate 1 / G / 10 U2m
Ampicillin
Plate 1 / G / 11 U3m
Ampicillin
Plate 1 / G / 12 U4m
Ampicillin
Plate 1 / H / 1 U5m
Ampicillin
Plate 1 / H / 2 U6m
Ampicillin
Plate 1 / H / 3 U7m
Ampicillin
Plate 1 / H / 4 U8m
Ampicillin
Plate 1 / H / 5 U9m
Ampicillin
Plate 1 / H / 6 U17m
Ampicillin
Plate 1 / H / 7 U19m
Ampicillin
Plate 1 / H / 8 U22m
Ampicillin
Plate 1 / H / 9 U24m
Ampicillin
Plate 1 / H / 10 U25m
Ampicillin
Plate 1 / H / 11 T1m
Ampicillin
Plate 1 / H / 12 T2m
Ampicillin
Plate 2 / A / 1 T7m
Ampicillin
Plate 2 / A / 2 T12m
Ampicillin
Plate 2 / A / 3 T13m
Ampicillin
Plate 2 / A / 4 T14m
Ampicillin
Plate 2 / A / 5 T15m
Ampicillin
Plate 2 / A / 6 T16m
Ampicillin
Plate 2 / A / 7 T17m
Ampicillin
Plate 2 / A / 8 T18m
Ampicillin
Plate 2 / A / 9 UC16m
Ampicillin
Plate 2 / A / 10 UC17m
Ampicillin
Plate 2 / A / 11 UC20m
Ampicillin
Plate 2 / A / 12 UCT1m
Ampicillin
Plate 2 / B / 1 V35m
Kanamycin
Plate 2 / B / 2 V37m
Kanamycin
Data calculated @ 2020-12-01

Kit Plate Map - #1000000161

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