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MoClo CRISPR/Cas Toolkit for Plants
(Kit # 1000000159 )

Depositing Lab:   Vladimir Nekrasov

The MoClo CRISPR/Cas Toolkit for Plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

A Modular Cloning Toolkit for Genome Editing in Plants Hahn F, Korolev A, Loures LS, Nekrasov V. BMC Plant Biology. 2020 Apr 23, 20(1):179; doi: 10.1186/s12870-020-02388-2. PubMed PMID: 32326901.

Description

Included in the MoClo CRISPR/Cas Toolkit for Plants are 95 plasmids to generate CRISPR constructs for genome editing in monocots and dicots. A variety of nucleases including SpCas9, SaCas9, FnCas12a, LbCas12a, xCas9 3.7, and SpCas9-NG allow for targeting of different PAM sites within the plant genome. Plasmids encoding both cytosine and adenine base editors are also included. Expression of these factors can be driven by Pol II promoters for use in both monocots and dicots. For expression of targeting sequences used by these different nucleases, this kit also includes gRNA, crRNA, and tRNA-gRNA backbones as well as Pol III promoters. Extensive protocols can be found in the original publication for the construction of plasmids for use in a number of different experimental systems.

See Kit Documentation under the Protocols & Resources tab for a complete list of the plasmids and inserts.

CRISPR Cas Construction

CRISPR/Cas constructs for genome engineering in monocots and dicots. A) Construct with a single sgRNA. B) Four sgRNAs in a single construct. Figure from Hahn et al. BMC Plant Biology (2020) 20:179 https://doi.org/10.1186/s12870-020-02388-2 licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).

Kit Documentation

Icon Kit 1000000159 Plasmids (24.8 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The MoClo CRISPR/Cas Toolkit for Plants was a gift from Vladimir Nekrasov (Addgene kit #1000000159).”

For your Reference section:

A Modular Cloning Toolkit for Genome Editing in Plants Hahn F, Korolev A, Loures LS, Nekrasov V. BMC Plant Biology. 2020 Apr 23, 20(1):179; doi: 10.1186/s12870-020-02388-2. PubMed PMID: 32326901.

MoClo CRISPR/Cas Toolkit for Plants - #1000000159

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin
Kanamycin
Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pFH13
Spectinomycin
A / 2 pFH14
Spectinomycin
A / 3 pFH15
Spectinomycin
A / 4 pFH16
Spectinomycin
A / 5 pFH17
Spectinomycin
A / 6 pFH22
Spectinomycin
A / 7 pFH32
Spectinomycin
A / 8 pFH45
Spectinomycin
A / 9 pFH46
Spectinomycin
A / 10 pFH47
Spectinomycin
A / 11 pFH48
Spectinomycin
A / 12 pFH55
Spectinomycin
B / 1 pFH76
Spectinomycin
B / 2 pFH77
Spectinomycin
B / 3 pFH79
Spectinomycin
B / 4 pFH91
Spectinomycin
B / 5 pFH92
Spectinomycin
B / 6 pFH40
Spectinomycin
B / 7 pFH41
Spectinomycin
B / 8 pFH42
Spectinomycin
B / 9 pFH56
Spectinomycin
B / 10 pFH57
Spectinomycin
B / 11 pFH31
Spectinomycin
B / 12 pFH33
Spectinomycin
C / 1 pFH34
Spectinomycin
C / 2 pFH35
Spectinomycin
C / 3 pFH36
Spectinomycin
C / 4 pFH37
Spectinomycin
C / 5 pFH38
Spectinomycin
C / 6 pFH39
Spectinomycin
C / 7 pFH43
Spectinomycin
C / 8 pFH44
Spectinomycin
C / 9 pFH24
Spectinomycin
C / 10 pFH25
Spectinomycin
C / 11 pAK-EL-01
Kanamycin
C / 12 pAK-EL-02
Kanamycin
D / 1 pAK-EL-03
Kanamycin
D / 2 pAK-EL-04
Kanamycin
D / 3 pAK-EL-05
Kanamycin
D / 4 pFH85
Kanamycin
D / 5 pFH86
Kanamycin
D / 6 pFH87
Kanamycin
D / 7 pFH88
Kanamycin
D / 8 pFH89
Kanamycin
D / 9 pFH90
Kanamycin
D / 10 pFH99
Kanamycin
D / 11 pFH100
Kanamycin
D / 12 pFH101
Kanamycin
E / 1 pFH102
Kanamycin
E / 2 pFH103
Kanamycin
E / 3 pFH104
Kanamycin
E / 4 pFH113
Kanamycin
E / 5 pFH49
Kanamycin
E / 6 pFH50
Kanamycin
E / 7 pFH51
Kanamycin
E / 8 pFH71
Kanamycin
E / 9 pAK002
Kanamycin
E / 10 pAK003
Kanamycin
E / 11 pAK004
Kanamycin
E / 12 pAK005
Kanamycin
F / 1 pAK006
Kanamycin
F / 2 pAK007
Kanamycin
F / 3 pFH72
Kanamycin
F / 4 pFH73
Kanamycin
F / 5 pFH74
Kanamycin
F / 6 pFH75
Kanamycin
F / 7 pAK008
Kanamycin
F / 8 pAK009
Kanamycin
F / 9 pAK010
Kanamycin
F / 10 pAK011
Kanamycin
F / 11 pAK012
Kanamycin
F / 12 pFH130
Kanamycin
G / 1 pFH131
Kanamycin
G / 2 pFH23
Ampicillin
G / 3 pFH52
Ampicillin
G / 4 pFH53
Ampicillin
G / 5 pFH54
Ampicillin
G / 6 pFH58
Ampicillin
G / 7 pFH59
Ampicillin
G / 8 pFH60
Ampicillin
G / 9 pFH61
Ampicillin
G / 10 pFH68
Ampicillin
G / 11 pFH69
Ampicillin
G / 12 pFH70
Ampicillin
H / 1 pFH80
Ampicillin
H / 2 pFH81
Ampicillin
H / 3 pFH82
Ampicillin
H / 4 pFH84
Ampicillin
H / 5 pFH93
Ampicillin
H / 6 pFH109
Ampicillin
H / 7 pFH114
Ampicillin
H / 8 pFH132
Ampicillin
H / 9 pFH133
Ampicillin
H / 10 pFH66
Ampicillin
H / 11 pFH67
Ampicillin
Data calculated @ 2020-10-23

Kit Plate Map - #1000000159

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