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Plasmid kit icon

Roth Lab PRESTO-Tango GPCR Kit
(Kit # 1000000068 )

Depositing Lab: Bryan Roth

The PRESTO-Tango Kit can be used to measure receptor activation for more than 300 GPCRs via a modified Tango beta-arrestin recruitment assay.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

For more information, visit the Roth Lab PRESTO-Tango web page which provides the codon-optimized DNA sequences and the protein sequences for each construct.

Add to Cart
$1150 USD + shipping

Available to academics and nonprofits only.

Printable Kit Description

Icon PRESTO-Tango GPCR Kit (76.1 KB)

Original Publication

PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguère PM, Sciaky N, Roth BL. Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. Epub 2015 Apr 20. PubMed .

Depositor-provided Files

Sequence Information: Icon Prototypical Tango Construct Sequence (12.3 KB)

Additional Reference

The Tango beta-arrestin recruitment assay was adapted for this kit:

The genetic design of signaling cascades to record receptor activation. Barnea G, Strapps W, Herrada G, Berman Y, Ong J, Kloss B, Axel R, Lee KJ. Proc Natl Acad Sci U S A 2008 Jan 8;105(1):64-9. doi: 10.1073/pnas.0710487105. Epub 2007 Dec 28. PubMed .

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The PRESTO-Tango plasmid kit was a gift from Bryan Roth (Addgene kit # 1000000068).”

For your Reference section:

PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguère PM, Sciaky N, Roth BL. Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. Epub 2015 Apr 20. PubMed .

PRESTO-Tango Kit - #1000000068

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 ADCYAP1R1-Tango
Ampicillin
Plate 1 / A / 2 ADRA2A-Tango
Ampicillin
Plate 1 / A / 3 APJ-Tango
Ampicillin
Plate 1 / A / 4 C5A-Tango
Ampicillin
Plate 1 / A / 5 CCR3-Tango
Ampicillin
Plate 1 / A / 6 CHRM1-Tango
Ampicillin
Plate 1 / A / 7 CRHR1-Tango
Ampicillin
Plate 1 / A / 8 CXCR6-Tango
Ampicillin
Plate 1 / A / 9 DRD5-Tango
Ampicillin
Plate 1 / A / 10 FFA1-Tango
Ampicillin
Plate 1 / A / 11 GAL1-Tango
Ampicillin
Plate 1 / A / 12 GLP2R-Tango
Ampicillin
Plate 1 / B / 1 ADORA1-Tango
Ampicillin
Plate 1 / B / 2 ADRA2B-Tango
Ampicillin
Plate 1 / B / 3 AVPR1A-Tango
Ampicillin
Plate 1 / B / 4 CALCRb-Tango
Ampicillin
Plate 1 / B / 5 CCR4-Tango
Ampicillin
Plate 1 / B / 6 CHRM2-Tango
Ampicillin
Plate 1 / B / 7 CRHR2-Tango
Ampicillin
Plate 1 / B / 8 CXCR7-Tango
Ampicillin
Plate 1 / B / 9 EDNRA-Tango
Ampicillin
Plate 1 / B / 10 FFA2-Tango
Ampicillin
Plate 1 / B / 11 GAL2-Tango
Ampicillin
Plate 1 / B / 12 GNRHR-Tango
Ampicillin
Plate 1 / C / 1 ADORA2A-Tango
Ampicillin
Plate 1 / C / 2 ADRA2C-Tango
Ampicillin
Plate 1 / C / 3 AVPR1B-Tango
Ampicillin
Plate 1 / C / 4 CALCRL-Tango
Ampicillin
Plate 1 / C / 5 CCR5-Tango
Ampicillin
Plate 1 / C / 6 CHRM3-Tango
Ampicillin
Plate 1 / C / 7 CX3C1-Tango
Ampicillin
Plate 1 / C / 8 CYSLTR1-Tango
Ampicillin
Plate 1 / C / 9 EDNRB-Tango
Ampicillin
Plate 1 / C / 10 FFA3-Tango
Ampicillin
Plate 1 / C / 11 GAL3-Tango
Ampicillin
Plate 1 / C / 12 GPBA-Tango
Ampicillin
Plate 1 / D / 1 ADORA2B-Tango
Ampicillin
Plate 1 / D / 2 ADRB1-Tango
Ampicillin
Plate 1 / D / 3 AVPR2-Tango
Ampicillin
Plate 1 / D / 4 CASR-Tango
Ampicillin
Plate 1 / D / 5 CCR6-Tango
Ampicillin
Plate 1 / D / 6 CHRM4-Tango
Ampicillin
Plate 1 / D / 7 CXCR1-Tango
Ampicillin
Plate 1 / D / 8 CYSLTR2-Tango
Ampicillin
Plate 1 / D / 9 ELTD1-Tango
Ampicillin
Plate 1 / D / 10 FPR1-Tango
Ampicillin
Plate 1 / D / 11 GCGR-Tango
Ampicillin
Plate 1 / D / 12 GPER-Tango
Ampicillin
Plate 1 / E / 1 ADORA3-Tango
Ampicillin
Plate 1 / E / 2 ADRB2-Tango
Ampicillin
Plate 1 / E / 3 BB3-Tango
Ampicillin
Plate 1 / E / 4 CCKAR-Tango
Ampicillin
Plate 1 / E / 5 CCR7-Tango
Ampicillin
Plate 1 / E / 6 CHRM5-Tango
Ampicillin
Plate 1 / E / 7 CXCR2-Tango
Ampicillin
Plate 1 / E / 8 DRD1-Tango
Ampicillin
Plate 1 / E / 9 F2R-Tango
Ampicillin
Plate 1 / E / 10 FPR2-Tango
Ampicillin
Plate 1 / E / 11 GHRHR-Tango
Ampicillin
Plate 1 / E / 12 GPR1-Tango
Ampicillin
Plate 1 / F / 1 ADRA1A-Tango
Ampicillin
Plate 1 / F / 2 ADRB3-Tango
Ampicillin
Plate 1 / F / 3 BDKBR1-Tango
Ampicillin
Plate 1 / F / 4 CCKBR-Tango
Ampicillin
Plate 1 / F / 5 CCR8-Tango
Ampicillin
Plate 1 / F / 6 CMKLR1-Tango
Ampicillin
Plate 1 / F / 7 CXCR3-Tango
Ampicillin
Plate 1 / F / 8 DRD2-Tango
Ampicillin
Plate 1 / F / 9 F2RL1-Tango
Ampicillin
Plate 1 / F / 10 FPR3-Tango
Ampicillin
Plate 1 / F / 11 GHSR-Tango
Ampicillin
Plate 1 / F / 12 GPR101-Tango
Ampicillin
Plate 1 / G / 1 ADRA1B-Tango
Ampicillin
Plate 1 / G / 2 AGTR1-Tango
Ampicillin
Plate 1 / G / 3 BDKBR2-Tango
Ampicillin
Plate 1 / G / 4 CCR10-Tango
Ampicillin
Plate 1 / G / 5 CCRL2-Tango
Ampicillin
Plate 1 / G / 6 CNR1-Tango
Ampicillin
Plate 1 / G / 7 CXCR4-Tango
Ampicillin
Plate 1 / G / 8 DRD3-Tango
Ampicillin
Plate 1 / G / 9 F2RL2-Tango
Ampicillin
Plate 1 / G / 10 FSHR-Tango
Ampicillin
Plate 1 / G / 11 GIPR-Tango
Ampicillin
Plate 1 / G / 12 GPR110-Tango
Ampicillin
Plate 1 / H / 1 ADRA1D-Tango
Ampicillin
Plate 1 / H / 2 AGTR2-Tango
Ampicillin
Plate 1 / H / 3 C3AR1-Tango
Ampicillin
Plate 1 / H / 4 CCR2-Tango
Ampicillin
Plate 1 / H / 5 CD97-Tango
Ampicillin
Plate 1 / H / 6 CNR2-Tango
Ampicillin
Plate 1 / H / 7 CXCR5-Tango
Ampicillin
Plate 1 / H / 8 DRD4-Tango
Ampicillin
Plate 1 / H / 9 F2RL3-Tango
Ampicillin
Plate 1 / H / 10 GABBR1-Tango
Ampicillin
Plate 1 / H / 11 GLP1R-Tango
Ampicillin
Plate 1 / H / 12 GPR111-Tango
Ampicillin
Plate 2 / A / 1 GPR113-Tango
Ampicillin
Plate 2 / A / 2 GPR124-Tango
Ampicillin
Plate 2 / A / 3 GPR143-Tango
Ampicillin
Plate 2 / A / 4 GPR152-Tango
Ampicillin
Plate 2 / A / 5 GPR17-Tango
Ampicillin
Plate 2 / A / 6 GPR20-Tango
Ampicillin
Plate 2 / A / 7 GPR32-Tango
Ampicillin
Plate 2 / A / 8 GPR45-Tango
Ampicillin
Plate 2 / A / 9 GPR63-Tango
Ampicillin
Plate 2 / A / 10 GPR83-Tango
Ampicillin
Plate 2 / A / 11 GPRC5C-Tango
Ampicillin
Plate 2 / A / 12 GRM7-Tango
Ampicillin
Plate 2 / B / 1 GPR114-Tango
Ampicillin
Plate 2 / B / 2 GPR125-Tango
Ampicillin
Plate 2 / B / 3 GPR144-Tango
Ampicillin
Plate 2 / B / 4 GPR153-Tango
Ampicillin
Plate 2 / B / 5 GPR171-Tango
Ampicillin
Plate 2 / B / 6 GPR21-Tango
Ampicillin
Plate 2 / B / 7 GPR34-Tango
Ampicillin
Plate 2 / B / 8 GPR50-Tango
Ampicillin
Plate 2 / B / 9 GPR64-Tango
Ampicillin
Plate 2 / B / 10 GPR84-Tango
Ampicillin
Plate 2 / B / 11 GPRC5D-Tango
Ampicillin
Plate 2 / B / 12 GRM8-Tango
Ampicillin
Plate 2 / C / 1 GPR115-Tango
Ampicillin
Plate 2 / C / 2 GPR126-Tango
Ampicillin
Plate 2 / C / 3 GPR146-Tango
Ampicillin
Plate 2 / C / 4 GPR156-Tango
Ampicillin
Plate 2 / C / 5 GPR173-Tango
Ampicillin
Plate 2 / C / 6 GPR22-Tango
Ampicillin
Plate 2 / C / 7 GPR35-Tango
Ampicillin
Plate 2 / C / 8 GPR52-Tango
Ampicillin
Plate 2 / C / 9 GPR65-Tango
Ampicillin
Plate 2 / C / 10 GPR85-Tango
Ampicillin
Plate 2 / C / 11 GPRC6A-Tango
Ampicillin
Plate 2 / C / 12 GRPR-Tango
Ampicillin
Plate 2 / D / 1 GPR116-Tango
Ampicillin
Plate 2 / D / 2 GPR132-Tango
Ampicillin
Plate 2 / D / 3 GPR148-Tango
Ampicillin
Plate 2 / D / 4 GPR157-Tango
Ampicillin
Plate 2 / D / 5 GPR174-Tango
Ampicillin
Plate 2 / D / 6 GPR25-Tango
Ampicillin
Plate 2 / D / 7 GPR37-Tango
Ampicillin
Plate 2 / D / 8 GPR55-Tango
Ampicillin
Plate 2 / D / 9 GPR68-Tango
Ampicillin
Plate 2 / D / 10 GPR87-Tango
Ampicillin
Plate 2 / D / 11 GRM1-Tango
Ampicillin
Plate 2 / D / 12 HCA1-Tango
Ampicillin
Plate 2 / E / 1 GPR119-Tango
Ampicillin
Plate 2 / E / 2 GPR133-Tango
Ampicillin
Plate 2 / E / 3 GPR149-Tango
Ampicillin
Plate 2 / E / 4 GPR158-Tango
Ampicillin
Plate 2 / E / 5 GPR18-Tango
Ampicillin
Plate 2 / E / 6 GPR26-Tango
Ampicillin
Plate 2 / E / 7 GPR37L1-Tango
Ampicillin
Plate 2 / E / 8 GPR56-Tango
Ampicillin
Plate 2 / E / 9 GPR75-Tango
Ampicillin
Plate 2 / E / 10 GPR88-Tango
Ampicillin
Plate 2 / E / 11 GRM2-Tango
Ampicillin
Plate 2 / E / 12 HCA2-Tango
Ampicillin
Plate 2 / F / 1 GPR12-Tango
Ampicillin
Plate 2 / F / 2 GPR135-Tango
Ampicillin
Plate 2 / F / 3 GPR15-Tango
Ampicillin
Plate 2 / F / 4 GPR160-Tango
Ampicillin
Plate 2 / F / 5 GPR182-Tango
Ampicillin
Plate 2 / F / 6 GPR27-Tango
Ampicillin
Plate 2 / F / 7 GPR39-Tango
Ampicillin
Plate 2 / F / 8 GPR6-Tango
Ampicillin
Plate 2 / F / 9 GPR77-Tango
Ampicillin
Plate 2 / F / 10 GPR97-Tango
Ampicillin
Plate 2 / F / 11 GRM4-Tango
Ampicillin
Plate 2 / F / 12 HCA3-Tango
Ampicillin
Plate 2 / G / 1 GPR120-Tango
Ampicillin
Plate 2 / G / 2 GPR141-Tango
Ampicillin
Plate 2 / G / 3 GPR150-Tango
Ampicillin
Plate 2 / G / 4 GPR161-Tango
Ampicillin
Plate 2 / G / 5 GPR183-Tango
Ampicillin
Plate 2 / G / 6 GPR3-Tango
Ampicillin
Plate 2 / G / 7 GPR4-Tango
Ampicillin
Plate 2 / G / 8 GPR61-Tango
Ampicillin
Plate 2 / G / 9 GPR78-Tango
Ampicillin
Plate 2 / G / 10 GPRC5A-Tango
Ampicillin
Plate 2 / G / 11 GRM5-Tango
Ampicillin
Plate 2 / G / 12 HCTR1-Tango
Ampicillin
Plate 2 / H / 1 GPR123-Tango
Ampicillin
Plate 2 / H / 2 GPR142-Tango
Ampicillin
Plate 2 / H / 3 GPR151-Tango
Ampicillin
Plate 2 / H / 4 GPR162-Tango
Ampicillin
Plate 2 / H / 5 GPR19-Tango
Ampicillin
Plate 2 / H / 6 GPR31-Tango
Ampicillin
Plate 2 / H / 7 GPR44-Tango
Ampicillin
Plate 2 / H / 8 GPR62-Tango
Ampicillin
Plate 2 / H / 9 GPR82-Tango
Ampicillin
Plate 2 / H / 10 GPRC5B-Tango
Ampicillin
Plate 2 / H / 11 GRM6-Tango
Ampicillin
Plate 2 / H / 12 HCTR2-Tango
Ampicillin
Plate 3 / A / 1 HRH1-Tango
Ampicillin
Plate 3 / A / 2 HTR1F-Tango
Ampicillin
Plate 3 / A / 3 KISSPEPTIN-R-Tango
Ampicillin
Plate 3 / A / 4 LTB4R2B-Tango
Ampicillin
Plate 3 / A / 5 MCHR1-Tango
Ampicillin
Plate 3 / A / 6 MRGPRX2-Tango
Ampicillin
Plate 3 / A / 7 NPBW1-Tango
Ampicillin
Plate 3 / A / 8 NPY5R-Tango
Ampicillin
Plate 3 / A / 9 OPRM1-Tango
Ampicillin
Plate 3 / A / 10 P2RY13-Tango
Ampicillin
Plate 3 / A / 11 PRRP-Tango
Ampicillin
Plate 3 / A / 12 PTGIR-Tango
Ampicillin
Plate 3 / B / 1 HRH2-Tango
Ampicillin
Plate 3 / B / 2 HTR2A-Tango
Ampicillin
Plate 3 / B / 3 LHCGR-Tango
Ampicillin
Plate 3 / B / 4 MAS1-Tango
Ampicillin
Plate 3 / B / 5 MCHR2-Tango
Ampicillin
Plate 3 / B / 6 MRGPRX3-Tango
Ampicillin
Plate 3 / B / 7 NPBW2-Tango
Ampicillin
Plate 3 / B / 8 NTSR1-Tango
Ampicillin
Plate 3 / B / 9 OXER1-Tango
Ampicillin
Plate 3 / B / 10 P2RY14-Tango
Ampicillin
Plate 3 / B / 11 PTAFR-Tango
Ampicillin
Plate 3 / B / 12 PTH1R-Tango
Ampicillin
Plate 3 / C / 1 HRH3-Tango
Ampicillin
Plate 3 / C / 2 HTR2B-Tango
Ampicillin
Plate 3 / C / 3 LPAR1-Tango
Ampicillin
Plate 3 / C / 4 MAS1L-Tango
Ampicillin
Plate 3 / C / 5 MLNR-Tango
Ampicillin
Plate 3 / C / 6 MRGPRX4-Tango
Ampicillin
Plate 3 / C / 7 NPFF1-Tango
Ampicillin
Plate 3 / C / 8 NTSR2-Tango
Ampicillin
Plate 3 / C / 9 OXGR1 -Tango
Ampicillin
Plate 3 / C / 10 P2RY2-Tango
Ampicillin
Plate 3 / C / 11 PTGDR-Tango
Ampicillin
Plate 3 / C / 12 PTH2R-Tango
Ampicillin
Plate 3 / D / 1 HRH4-Tango
Ampicillin
Plate 3 / D / 2 HTR2C-Tango
Ampicillin
Plate 3 / D / 3 LPAR2-Tango
Ampicillin
Plate 3 / D / 4 MC1R-Tango
Ampicillin
Plate 3 / D / 5 MRGPRD-Tango
Ampicillin
Plate 3 / D / 6 MTNR1A-Tango
Ampicillin
Plate 3 / D / 7 NPFF2-Tango
Ampicillin
Plate 3 / D / 8 OPN3-Tango
Ampicillin
Plate 3 / D / 9 OXTR-Tango
Ampicillin
Plate 3 / D / 10 P2RY4-Tango
Ampicillin
Plate 3 / D / 11 PTGER1-Tango
Ampicillin
Plate 3 / D / 12 QRFP-Tango
Ampicillin
Plate 3 / E / 1 HTR1A-Tango
Ampicillin
Plate 3 / E / 2 HTR4-Tango
Ampicillin
Plate 3 / E / 3 LPAR4-Tango
Ampicillin
Plate 3 / E / 4 MC2R-Tango
Ampicillin
Plate 3 / E / 5 MRGPRE-Tango
Ampicillin
Plate 3 / E / 6 MTNR1B-Tango
Ampicillin
Plate 3 / E / 7 NPS-Tango
Ampicillin
Plate 3 / E / 8 OPN5-Tango
Ampicillin
Plate 3 / E / 9 P2RY1-Tango
Ampicillin
Plate 3 / E / 10 P2RY6-Tango
Ampicillin
Plate 3 / E / 11 PTGER2-Tango
Ampicillin
Plate 3 / E / 12 RXFP1-Tango
Ampicillin
Plate 3 / F / 1 HTR1B-Tango
Ampicillin
Plate 3 / F / 2 HTR5-Tango
Ampicillin
Plate 3 / F / 3 LPAR5-Tango
Ampicillin
Plate 3 / F / 4 MC3R-Tango
Ampicillin
Plate 3 / F / 5 MRGPRF-Tango
Ampicillin
Plate 3 / F / 6 NMBR-Tango
Ampicillin
Plate 3 / F / 7 NPY1R-Tango
Ampicillin
Plate 3 / F / 8 OPRD1-Tango
Ampicillin
Plate 3 / F / 9 P2RY10-Tango
Ampicillin
Plate 3 / F / 10 P2RY8-Tango
Ampicillin
Plate 3 / F / 11 PTGER3-Tango
Ampicillin
Plate 3 / F / 12 RXFP2-Tango
Ampicillin
Plate 3 / G / 1 HTR1D-Tango
Ampicillin
Plate 3 / G / 2 HTR6-Tango
Ampicillin
Plate 3 / G / 3 LPAR6-Tango
Ampicillin
Plate 3 / G / 4 MC4R-Tango
Ampicillin
Plate 3 / G / 5 MRGPRG-Tango
Ampicillin
Plate 3 / G / 6 NMUR1-Tango
Ampicillin
Plate 3 / G / 7 NPY2R-Tango
Ampicillin
Plate 3 / G / 8 OPRK1-Tango
Ampicillin
Plate 3 / G / 9 P2RY11-Tango
Ampicillin
Plate 3 / G / 10 PK1-Tango
Ampicillin
Plate 3 / G / 11 PTGER4-Tango
Ampicillin
Plate 3 / G / 12 RXFP3-Tango
Ampicillin
Plate 3 / H / 1 HTR1E-Tango
Ampicillin
Plate 3 / H / 2 HTR7-Tango
Ampicillin
Plate 3 / H / 3 LTB4R-Tango
Ampicillin
Plate 3 / H / 4 MC5R-Tango
Ampicillin
Plate 3 / H / 5 MRGPRX1-Tango
Ampicillin
Plate 3 / H / 6 NMUR2-Tango
Ampicillin
Plate 3 / H / 7 NPY4R-Tango
Ampicillin
Plate 3 / H / 8 OPRL1-Tango
Ampicillin
Plate 3 / H / 9 P2RY12-Tango
Ampicillin
Plate 3 / H / 10 PK2-Tango
Ampicillin
Plate 3 / H / 11 PTGFR-Tango
Ampicillin
Plate 3 / H / 12 RXFP4-Tango
Ampicillin
Plate 4 / A / 1 S1PR1-Tango
Ampicillin
Plate 4 / A / 2 SSTR3-Tango
Ampicillin
Plate 4 / A / 3 TAAR8-Tango
Ampicillin
Plate 4 / A / 4 VIPR1-Tango
Ampicillin
Plate 4 / B / 1 S1PR2-Tango
Ampicillin
Plate 4 / B / 2 SSTR4-Tango
Ampicillin
Plate 4 / B / 3 TAAR9-Tango
Ampicillin
Plate 4 / B / 4 VIPR2-Tango
Ampicillin
Plate 4 / C / 1 S1PR3-Tango
Ampicillin
Plate 4 / C / 2 SSTR5-Tango
Ampicillin
Plate 4 / C / 3 TACR1-Tango
Ampicillin
Plate 4 / D / 1 S1PR4-Tango
Ampicillin
Plate 4 / D / 2 SUCNR1-Tango
Ampicillin
Plate 4 / D / 3 TACR2-Tango
Ampicillin
Plate 4 / E / 1 S1PR5-Tango
Ampicillin
Plate 4 / E / 2 TA1-Tango
Ampicillin
Plate 4 / E / 3 TACR3-Tango
Ampicillin
Plate 4 / F / 1 SCTR-Tango
Ampicillin
Plate 4 / F / 2 TAAR2-Tango
Ampicillin
Plate 4 / F / 3 TBXA2R-Tango
Ampicillin
Plate 4 / G / 1 SSTR1-Tango
Ampicillin
Plate 4 / G / 2 TAAR5-Tango
Ampicillin
Plate 4 / G / 3 TSHR-Tango
Ampicillin
Plate 4 / H / 1 SSTR2-Tango
Ampicillin
Plate 4 / H / 2 TAAR6-Tango
Ampicillin
Plate 4 / H / 3 UTS2R-Tango
Ampicillin
Data calculated @ 2017-06-26

Kit Plate Map - #1000000068

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