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MoClo Plant Parts II and Infrastructure Kit
(Kit # 1000000135 )

Depositing Lab:   Johannes Stuttmann

The MoClo Plant Parts II and Infrastructure Kit contains 95 DNA modules compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. Gantner J., Ordon J., Ilse T., Kretschmer C., Gruetzner R., Lofke C., Dagdas Y., Burstenbinder K., Marillonnet S. and Stuttmann J. PLoS ONE (2018), 13(5), e0197185. May 30; DOI: 10.1371/journal.pone.0197185. PubMed PMID: 29847550.

Link to original publication

Description

The MoClo Plant Parts II and Infrastructure Kit contains 95 DNA modules compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs. Most modules are fully or partially compatible with the GoldenBraid standard.

This kit contains approximately 80 Level 0 modules (phytobricks; e.g. promoters, epitope tags, subcellular localization signals, genes). A further set of Level 0 modules is intended for the assembly of Gateway destination vectors by MoClo. A set of shuttle vectors allows the conversion of MoClo modules containing coding sequences into Gateway entry vectors. Furthermore, the kit contains eight vectors for yeast two hybrid assays or type III secretion system-dependent bacterium-to-plant delivery of proteins compatible with the MoClo system (vectors receive CDS1 or CDS1ns modules). The kit also contains a Golden Gate cloning-compatible TRV-RNA2 vector for virus-induced gene silencing.

Please review Supplemental Tables S2 and S3 from the original publication for more details on using plamsids included in this kit.

Addgene_MoClo-Toolkit-scheme.png
  • The Modular Cloning standard allows Golden Gate assembly of multigene constructs from standardized parts (phytobricks). This kit contains new phytobricks, and also vectors to connect Modular Cloning and Gateway Cloning platforms. Furthermore, the kit contains yeast two hybrid and bacterium-to-plant translocation vectors designed to receive CDS1 and CDS1ns modules of the MoClo system.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The MoClo Plant Parts II and Infrastructure Kit was a gift from Johannes Stuttmann (Addgene kit #1000000135).”

For your Reference section:

Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system. Gantner J., Ordon J., Ilse T., Kretschmer C., Gruetzner R., Lofke C., Dagdas Y., Burstenbinder K., Marillonnet S. and Stuttmann J. PLoS ONE (2018), 13, e0197185. 2013 May 30; DOI: 10.1371/journal.pone.0197185. PubMed PMID: 29847550.

MoClo Plant Parts II and Infrastructure Kit - #1000000135

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Chloramphenicol and Kanamycin
Chloramphenicol and Spectinomycin
Ampicillin
Kanamycin
Chloramphenicol and Ampicillin
Chloramphenicol and Gentamycin
Spectinomycin

Inventory

Well Plasmid Resistance
A / 1 pJOG130
Chloramphenicol and Kanamycin
A / 2 pJOG131
Chloramphenicol and Kanamycin
A / 3 pJOG387
Chloramphenicol and Spectinomycin
A / 4 pJOG267
Chloramphenicol and Spectinomycin
A / 5 pJOG562
Chloramphenicol and Kanamycin
A / 6 pJOG417
Ampicillin
A / 7 pJOG418
Kanamycin
A / 8 pCK011
Chloramphenicol and Ampicillin
A / 9 pCK012
Chloramphenicol and Kanamycin
A / 10 pCK013
Chloramphenicol and Gentamycin
A / 11 pCK014
Chloramphenicol and Gentamycin
A / 12 pCK015
Chloramphenicol and Gentamycin
B / 1 pCK016
Chloramphenicol and Gentamycin
B / 2 pTRV2-GG
Spectinomycin
B / 3 pTEI036
Spectinomycin
B / 4 pTEI042
Spectinomycin
B / 5 pJOG644
Spectinomycin
B / 6 pTEI038
Spectinomycin
B / 7 pJOG640
Spectinomycin
B / 8 pJOG684
Spectinomycin
B / 9 pJOG603
Spectinomycin
B / 10 pTEI044
Spectinomycin
B / 11 pJOG301
Spectinomycin
B / 12 pAGM28521
Spectinomycin
C / 1 pJOG299
Spectinomycin
C / 2 pJOG298
Spectinomycin
C / 3 pJOG295
Spectinomycin
C / 4 pJOG300
Spectinomycin
C / 5 pJOG025
Spectinomycin
C / 6 pJOG123
Spectinomycin
C / 7 pJOG026
Spectinomycin
C / 8 pJOG124
Spectinomycin
C / 9 pJOG466
Spectinomycin
C / 10 pJOG467
Spectinomycin
C / 11 pJOG010
Spectinomycin
C / 12 pJOG648
Spectinomycin
D / 1 pTEI067
Spectinomycin
D / 2 pTEI068
Spectinomycin
D / 3 pTEI069
Spectinomycin
D / 4 pTEI070
Spectinomycin
D / 5 pTEI071
Spectinomycin
D / 6 pTEI73
Spectinomycin
D / 7 pCK019
Spectinomycin
D / 8 pCK020
Spectinomycin
D / 9 pCK021
Spectinomycin
D / 10 pAGM1761
Spectinomycin
D / 11 pJOG020
Spectinomycin
D / 12 pJOG575
Spectinomycin
E / 1 pJOG022
Spectinomycin
E / 2 pJOG213
Spectinomycin
E / 3 pJOG004
Spectinomycin
E / 4 pJOG535
Spectinomycin
E / 5 pJOG577
Spectinomycin
E / 6 pJOG638
Spectinomycin
E / 7 pJOG639
Spectinomycin
E / 8 pJOG170
Spectinomycin
E / 9 pJOG176
Spectinomycin
E / 10 pJOG169
Spectinomycin
E / 11 pJOG175
Spectinomycin
E / 12 pJOG168
Spectinomycin
F / 1 pJOG174
Spectinomycin
F / 2 pJOG142
Spectinomycin
F / 3 pJOG143
Spectinomycin
F / 4 pJOG140
Spectinomycin
F / 5 pJOG141
Spectinomycin
F / 6 pJOG824
Spectinomycin
F / 7 pAGM16456
Spectinomycin
F / 8 pAGM16467
Spectinomycin
F / 9 pAGM16418
Spectinomycin
F / 10 pAGM16429
Spectinomycin
F / 11 pJOG642
Spectinomycin
F / 12 pJOG317
Spectinomycin
G / 1 pJOG331
Spectinomycin
G / 2 pJOG658
Spectinomycin
G / 3 pJOG659
Spectinomycin
G / 4 pJOG643
Spectinomycin
G / 5 pCK032
Spectinomycin
G / 6 pTEI037
Spectinomycin
G / 7 pTEI041
Spectinomycin
G / 8 pCK025
Spectinomycin
G / 9 pAGM16841
Spectinomycin
G / 10 pJOG173
Spectinomycin
G / 11 pJOG172
Spectinomycin
G / 12 pJOG171
Spectinomycin
H / 1 pJOG021
Spectinomycin
H / 2 pJOG351
Spectinomycin
H / 3 pJOG031
Spectinomycin
H / 4 pJOG536
Spectinomycin
H / 5 pJOG545
Spectinomycin
H / 6 pCK039
Spectinomycin
H / 7 pCK038
Spectinomycin
H / 8 pJOG641
Spectinomycin
H / 9 pTEI039
Spectinomycin
H / 10 pCK26
Spectinomycin
H / 11 pJOG822
Spectinomycin
H / 12 pJOG587
Spectinomycin
Data calculated @ 2018-12-09

Kit Plate Map - #1000000135

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