Constitutive Lentiviral RNAi Vectors
The laboratories of David Root (Broad Institute), Robert Weinberg (Whitehead Institute), Stephan Kissler (MIT), Luk Van Parijs (MIT), and Didier Trono (EPFL) have deposited lentiviral vectors for shRNA expression.
The pLKO.1 system is used by The RNAi Consortium for their shRNA Library.
| ID | Plasmid | Description |
|---|---|---|
| 10878 | pLKO.1 – TRC cloning vector | Lentiviral vector for shRNA expression. Recommended for cloning new shRNAs (contains a 1.9 kb stuffer region to easily visualize products from an AgeI/EcoRI restriction digest). |
| 10879 | pLKO.1 – TRC control | Negative control vector containing non-hairpin insert. |
| 1864 | pLKO.1 – scramble shRNA | Negative control vector with scrambled shRNA. |
| 11795 | pLL3.7 | Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. This vector is designed for inducing RNA interference in a wide range of cell types, tissues and organisms. It has been used to infect and efficiently silence proteins in hematopoetic stem cells and their progeny, and has been used to infect embryonic stem cells and single cell embryos to create transgenic animals. |
| 11619 | pLB | pLB is a modification of pLL3.7. Genetic elements known to prevent epigenetic silencing were added. |
| 12247 | pLVTHM | Lentiviral vector that expresses shRNA under the H1 promoter. An shRNA can be directly cloned into this vector, or an H1-shRNA cassette from another vector (e.g. pSUPER) can be cloned into this vector. Contains a 5’LTR and should be packaged using the 2nd generation packaging system (e.g. psPAX2). |
More information:
- Packaging Plasmids that can be used with the pLKO, pLL, and pLVTHM systems.
- “Addgene’s pLKO.1” Protocol for using the pLKO.1 vector in your experiments.
- pLL3.7 protocols from the Tyler Jacks laboratory at MIT.
- Trono Lab website or Lentiweb: information and a discussion forum on cloning, packaging, and other protocols.
- Moffat J et. al. 2006. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 124:1283-1298. (PubMed)
- Stewart SA et. al. 2003. Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA 9(4):493-501. (PubMed)
- Kissler et. al. 2006. In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Nature Genetics. 2006 Apr;38(4):479-83. (PubMed)
- Rubinson et. al. 2003. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nature Genetics 2003 Mar;33(3):401-6. (PubMed)
- Related plasmids from the laboratory of Robert Weinberg, Didier Trono, and Inder Verma.